Endophytic bacillus velezensis and application of fermentation liquor of endophytic bacillus velezensis in prevention and treatment of bemisia tabaci

The use of o-carboxybenzaldehyde, tebufenozide, and succinylcholine in the fermentation broth of Bacillus vesiculosus NM153 solved the problem of whitefly damage to tobacco leaves, achieving highly efficient control and demonstrating the potential for application as a biological pesticide.

CN121320142APending Publication Date: 2026-01-13HUNAN INST OF MICROBIOLOGY
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Patent Information

Application Number
CN202511387369.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-26
Publication Date
2026-01-13

AI Technical Summary

Technical Problem

Whiteflies cause serious damage to tobacco leaves, including feeding on sap, inducing sooty mold, and spreading viruses, and current technologies are insufficient to effectively control them.

Method used

Bacillus belysin NM153 and its fermentation broth, containing o-carboxybenzaldehyde, tebufenozide and succinylcholine, were prepared by shaking activation and fermentation. The mass ratio of these substances in the fermentation broth was 1:1-5:2-6, and the total mass fraction was greater than or equal to 3%. This broth was used to control whiteflies.

Benefits of technology

The fermentation broth of Bacillus belye NM153 significantly improved the mortality rate of whiteflies, especially the supernatant treatment, which was more effective than the bacterial suspension. It has the potential to quickly kill insects and effectively suppress whiteflies, and has the potential to be developed into a biological pesticide.

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Abstract

The invention belongs to the technical field of microbial fermentation and insect pest prevention and control, and particularly relates to endogenous bacillus velezensis and application of fermentation liquor of the endogenous bacillus velezensis in prevention and control of bemisia tabaci. The invention discloses bacillus velezensis NM153 which is preserved in the China Center for Type Culture Collection on March 27, 2025, and the preservation number of the bacillus velezensis NM153 is CCTCC (China Center for Type Culture Collection) M 2025605. A stock solution of the bacillus velezensis NM153 supernate and the bacterial suspension is diluted by 300 times and 500 times to treat the bemisia tabaci, and the result shows that the death rate of the bemisia tabaci treated by the bacillus velezensis NM153 supernate is obviously higher than that of a bacterial suspension treatment group, and the death rate of the bemisia tabaci treated by the bacterial suspension treatment group is obviously higher than that of the bemisia tabaci treated by the bacillus velezensis NM153. In addition, the bacillus velezensis NM153 metabolite is further verified and explained to have a remarkable technical effect on preventing and treating bemisia tabaci in o-carboxybenzaldehyde, tenuitoxin and micronomicin in the bacillus velezensis NM153 metabolite.
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Citation Information

Cited By

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