Preparation method of high-activity stephania tetrandra extract

Abstract

The invention discloses a preparation method of a high-activity stephania tetrandra extract, and aims to provide a preparation method of a stephania tetrandra extract rich in alkaloid and flavone. The method sequentially comprises the following steps: 1) crushing and sieving a stephania tetrandra medicinal material, adding an ethanol water solution according to a solid-to-liquid ratio of 1: (20-30), soaking for 10-20 minutes, adding salt which accounts for 15-35% of the weight of the solution, and uniformly stirring; (2) carrying out high-pressure homogenization wall breaking under the pressure of 400-1000bar, and starting condensation circulation for 2-4 times; 3) taking the mixed solution obtained in the step 2), adding activated clay accounting for 1-5% of the weight of the mixed solution at the temperature of 60-80 DEG C, stirring for 0.5-2 hours, standing overnight, and taking an ethanol layer; 4) adding 0.5-3% by weight of neutral alumina into the ethanol layer, and stirring for 0.5-1.0 h at the temperature of 25-60 DEG C, and 5) filtering by using a 0.2 [mu] m laminated filter, collecting the filtrate, concentrating the filtrate into an extract, adding water for redissolving, and then carrying out spray drying to obtain the stephania tetrandra extract, belonging to the technical field of cosmetics.

Description

TECHNICAL FIELD

[0001] The present application relates to a preparation method of a plant extract, in particular, to a preparation method of a high-activity extract of Stephania tetrandra S. Moore, and belongs to the technical field of cosmetics. BACKGROUND

[0002] With the deterioration of the environment and the intensification of the overuse of cosmetics, the incidence of skin problems such as skin sensitivity and acne is rising. These problems not only affect people's quality of daily life, but also can lead to more serious health problems. At present, the acne-removing skin care products on the market are mainly divided into two categories: one is the drug type mainly using antibiotics and retinoids, which has a quick effect, but is easy to produce drug resistance and may cause irritation and adverse reactions when used in high concentration; the other is the product mainly added with traditional Chinese medicine, which has a good repair effect, but the effect is relatively slow, and some products have a rough appearance and easily cause skin pigmentation.

[0003] In recent years, natural plant extracts have become a hot topic in the development of personal care products due to their safety and traditional Chinese medicine heritage. However, the stability of the plant extracts currently used in skin care products is low, which easily leads to a decrease in efficacy during the shelf life. In addition, some plant extracts such as the effective components in Stephania tetrandra S. Moore are not only difficult to extract completely, but also have the problem of low bioavailability. Therefore, how to effectively extract and utilize the active ingredients in natural plants while ensuring their stability and bioavailability has become a problem to be solved.

[0004] For the important traditional Chinese medicinal material Stephania tetrandra S. Moore, the existing extraction methods have some limitations. The traditional extraction method may produce a large amount of wastewater, polluting the environment, and the alkaloids are difficult to precipitate completely, reducing the extraction rate. Although the method of using ethanol reflux extraction can extract some active ingredients, the solvent loss is large and the cost is high. Therefore, it is of great significance to develop a method that can efficiently extract alkaloids and flavonoids from Stephania tetrandra S. Moore while avoiding the use of acid and alkali reagents, shortening the preparation time and improving the utilization of active substances. SUMMARY

[0005] In view of the above shortcomings, the purpose of the present application is to provide a preparation method of a Stephania tetrandra S. Moore extract rich in alkaloids and flavonoids.

[0006] To this end, the technical solution provided by the present application is as follows:

[0007] A preparation method of a high-activity extract of Stephania tetrandra S. Moore, comprising the following steps in sequence:

[0008] 1) The medicinal material of Stephania tetrandra S. Moore is crushed and sieved, and an ethanol aqueous solution is added according to a solid-liquid ratio of 1:20-30, and after soaking for 10-20 min, 15-35% of the weight of the solution is added, and the mixture is stirred uniformly;

[0009] 2) Start the condensation cycle device, control the temperature at 8-10 DEG C, and perform high-pressure homogenization under the pressure of 400-1000 bar;

[0010] 3) Take the mixed solution obtained in step 2), add 1-5% of activated white clay by weight of the mixed solution at the temperature of 60-80 DEG C, stir for 0.5-2 h, and then stand overnight, take the ethanol layer, and remove the water phase layer;

[0011] 4) Add 0.5-3% of neutral alumina by weight to the ethanol layer, and stir at the temperature of 25-60 DEG C for 0.5-1.0 h;

[0012] 5) 0.2 mu layer filter, concentrate the filtrate into extract, and then spray dry after redissolving with water to obtain light yellow-light brown powder of the extract of Stephania tetrandra.

[0013] Further, in the preparation method of the high-activity powder of Stephania tetrandra extract, the salt is one or a combination of phosphate and sulfate.

[0014] Further, in the preparation method of the high-activity powder of Stephania tetrandra extract, the sulfate is ammonium sulfate or potassium sulfate.

[0015] Further, in the preparation method of the high-activity powder of Stephania tetrandra extract, the ethanol aqueous solution is an ethanol aqueous solution with the volume fraction of 10-50% of ethanol.

[0016] Further, in the preparation method of the high-activity powder of Stephania tetrandra extract, step 2) is specifically: start the condensation cycle device, control the temperature at 10 DEG C, perform high-pressure homogenization under the pressure of 400-1000 bar for 2-3 min, and perform the high-pressure homogenization operation for 2-4 times.

[0017] Further, in the preparation method of the high-activity powder of Stephania tetrandra extract, the pore size of the filter membrane used in the filtration is 0.2 mu.

[0018] Compared with the prior art, the present application has the following beneficial effects:

[0019] 1. The present application adopts the high-pressure homogenization technology, cooperates with the ethanol-salt system extraction method, significantly improves the extraction rate of the active material of Stephania tetrandra, improves the bioavailability of the active material, solves the problem that the alkaloid is difficult to completely precipitate in the traditional extraction method, and effectively overcomes the disadvantage of large environmental pollution;

[0020] 2. The present application extracts by adding the ethanol aqueous solution and the salt, avoids using acid and alkali reagents, realizes the green environmental protection goal, reduces the solvent loss, reduces the production cost, and improves the purity and safety of the product;

[0021] 3. This invention employs a stepwise extraction process, including high-pressure homogenization and cell wall disruption, activated clay stirring, filtration and concentration, etc., which simplifies the preparation process, improves production efficiency, and is easy to implement for industrial production;

[0022] 4. This invention further improves the stability of the extract by adding neutral alumina for post-treatment, solving the problem of low stability of plant extracts in skin care products in the prior art and extending the shelf life of the product;

[0023] 5. This invention employs a 0.2μm stacked filter technology combined with a spray drying process to obtain high-purity Stephania tetrandra extract, ensuring product quality and efficacy, and exhibiting excellent powder properties;

[0024] 6. The Stephania tetrandra extract prepared by this invention has significant anti-acne and soothing effects, and can be used as a highly effective skin care additive, which has important application value. Attached Figure Description

[0025] Figure 1 The standard curve for tetrandrine;

[0026] Figure 2 The standard curve for tetrandrine;

[0027] Figure 3 This is the standard curve of total flavonoids (calculated as rutin);

[0028] Figure 4 This is a graph showing the changes in blood vessels in chicken embryos after 5 minutes of destimulation experiment using 0.05% SDS;

[0029] Figure 5 This is a graph showing the changes in blood vessels in chicken embryos after 5 minutes of an experiment using the Stephania tetrandra extract provided in Example 1 to reduce irritation.

[0030] Figure 6 This is a graph showing the inhibitory effect of Stephania tetrandra extract on the cellular inflammatory factor NO. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of the present invention clearer, preferred embodiments are selected below to further describe the present invention in detail. However, the scope of protection of the present invention is not limited to the scope shown in the embodiments.

[0032] Unless otherwise specified, all percentages below refer to mass percentages.

[0033] Example 1

[0034] (1) Take 500g of Fangji medicinal powder, add 30% ethanol aqueous solution according to the material-liquid ratio of 1:30, soak for 15 minutes, then add 25% ammonium sulfate by weight of the solution and stir evenly.

[0035] (2) Turn on the external condensation circulation device, control the temperature at 10℃, and perform high pressure homogenization and cell disruption at 800 bar for 3 minutes. Repeat the high pressure homogenization and cell disruption operation 3 times.

[0036] (3) Take the mixed solution from step (2), add 2% of the solution weight of activated clay at 70°C, stir for 1.0 h, let stand overnight, take the ethanol layer, and remove the aqueous phase layer.

[0037] (4) Add 2% neutral alumina by weight of ethanol layer to ethanol layer, stir at 50℃ for 1.0h, filter with 0.2μm CT-300 stacked filter, collect filtrate, concentrate the collected filtrate under reduced pressure to paste, vacuum degree 0.08MPa, concentration temperature 65℃, add 4 times the weight of water of the concentrate to reconstitute, spray dry to obtain light yellow powder Stephania tetrandra extract.

[0038] Example 2

[0039] (1) Take 500g of Fangji medicinal powder, add 25% ethanol aqueous solution at a material-to-liquid ratio of 1:25, soak for 15 minutes, then add 20% dipotassium hydrogen phosphate by weight of the solution and stir evenly.

[0040] (2) Turn on the external condensation circulation device, control the temperature at 10℃, perform high pressure homogenization and cell disruption at 900 bar for 3 minutes, and repeat the high pressure homogenization and cell disruption operation 4 times.

[0041] (3) Take the mixed solution from step (2), add 3% of the solution weight of activated clay at 60°C, stir for 1.0 h, let stand overnight, take the ethanol layer, and remove the aqueous phase layer.

[0042] (4) Add 1.5% of neutral alumina by weight of ethanol layer to ethanol layer, stir at 60℃ for 0.5h, filter with 0.2μm CT-300 stacked filter, collect filtrate, concentrate the collected filtrate under reduced pressure to paste, vacuum degree 0.08MPa, concentration temperature 65℃, add 4 times the weight of water of the concentrate to redissolve, spray dry to obtain light yellow powdered Stephania tetrandra extract.

[0043] Example 3

[0044] (1) Take 500g of Fangji medicinal powder, add 15% ethanol aqueous solution at a material-to-liquid ratio of 1:30, soak for 15 minutes, then add 30% potassium sulfate by weight of the solution and stir evenly.

[0045] (2) Turn on the external condensation circulation device, control the temperature at 10℃, perform high pressure homogenization and cell disruption at 500 bar for 3 minutes, and repeat the high pressure homogenization and cell disruption operation 3 times.

[0046] (3) Take the mixed solution from step (2), add 3% of the solution weight of activated clay at 70°C, stir for 2 hours, let stand overnight, take the ethanol layer, and remove the aqueous phase layer.

[0047] (4) Add 0.5% of neutral alumina by weight of ethanol layer to ethanol layer, stir at 60℃ for 1.0h, filter with 0.2μm CT-300 stacked filter, collect filtrate, concentrate to paste, redissolve with water, spray dry to obtain light yellow powdered Stephania tetrandra extract.

[0048] Comparative Example 1

[0049] (1) Take 500g of Fangji medicinal powder, add 24% ethanol aqueous solution at a material-to-liquid ratio of 1:30, soak for 15 minutes, then add 20% potassium sulfate by weight of the solution and stir evenly.

[0050] (2) At 80℃, add 3% of the solution weight of activated clay, stir for 0.5h, let stand overnight, take the ethanol layer and remove the aqueous phase layer.

[0051] (3) Add 3% by weight of neutral alumina to the ethanol layer, stir at 30°C for 0.5 h, filter with a 0.2 μm CT-300 stacked filter, collect the filtrate, concentrate the collected filtrate under reduced pressure to a paste state, vacuum degree 0.08 MPa, concentration temperature 65°C, add 4 times the weight of water to redissolve, spray dry to obtain brown powder Stephania tetrandra extract.

[0052] Comparative Example 2

[0053] (1) Take 500g of Fangji medicinal powder, add 50% ethanol aqueous solution according to the material-liquid ratio of 1:25, stir evenly, and soak for 15min.

[0054] (2) Turn on the external condensation circulation device, control the temperature at 10℃, and perform high-pressure homogenization and cell disruption at 600 bar. Repeat the high-pressure homogenization and cell disruption operation 4 times.

[0055] (3) Take the mixed solution from step (2), add 3% of the solution weight of activated clay at 60℃, stir for 1.0 h, centrifuge, and take the supernatant.

[0056] (4) Add 1.5% neutral alumina by weight of ethanol layer to ethanol layer, stir at 60℃ for 0.5h, filter with 0.2μm CT-300 stacked filter, collect filtrate, concentrate the collected filtrate under reduced pressure to paste, vacuum degree 0.08MPa, concentration temperature 65℃, add 4 times the weight of water of the concentrate to reconstitute, spray dry to obtain brown powder Stephania tetrandra extract.

[0057] Comparative Example 3

[0058] (1) Take 500g of Fangji medicinal powder, add 30% ethanol aqueous solution at a material-to-liquid ratio of 1:30, soak for 15 minutes, then add 25% disodium hydrogen phosphate by weight of the solution, and stir evenly.

[0059] (2) Turn on the external condensation circulation device, control the temperature at 10°C, and perform high-pressure homogenization and cell disruption at 800 bar. Repeat the high-pressure homogenization and cell disruption operation 3 times.

[0060] (3) Take the mixed solution from step (2), stir it at 70°C for 1.0 h, let it stand overnight, take the ethanol layer, and remove the aqueous phase layer.

[0061] (4) Add 2% neutral alumina by weight of ethanol layer to ethanol layer, stir at 50℃ for 1.0h, filter with 0.2μm CT-300 stacked filter, collect filtrate, concentrate the collected filtrate under reduced pressure to paste, vacuum degree 0.08MPa, concentration temperature 65℃, add 4 times the weight of water of the concentrate to reconstitute, spray dry to obtain light brown powdered Stephania tetrandra extract.

[0062] Comparative Example 4

[0063] (1) Take 500g of Fangji medicinal powder, add 30% ethanol aqueous solution at a material-to-liquid ratio of 1:30, soak for 15 minutes, then add 25% potassium dihydrogen phosphate by weight of the solution, and stir evenly.

[0064] (2) Turn on the external condensation circulation device, control the temperature at 10°C, and perform high-pressure homogenization and cell disruption at 800 bar. Repeat the high-pressure homogenization and cell disruption operation 3 times.

[0065] (3) Take the mixed solution from step (2), add 2% activated clay by weight of the solution at 70°C, stir for 1.0 h, let stand overnight, take the ethanol layer, and remove the aqueous phase layer.

[0066] (4) The filtrate was filtered using a 0.2 μm CT-300 stacked filter, and the collected filtrate was concentrated under reduced pressure to a paste state at a vacuum degree of 0.08 MPa and a concentration temperature of 65°C. The filtrate was then redissolved with 4 times the weight of water and spray-dried to obtain a light brown powdery Stephania tetrandra extract.

[0067] Comparative Example 5

[0068] (1) Take 500g of Fangji medicinal powder, add 30% ethanol aqueous solution at a material-to-liquid ratio of 1:30, soak for 15 minutes, then add 25% ammonium sulfate by weight of the solution and stir evenly.

[0069] (2) Turn on the external condensation circulation device, control the temperature at 10°C, and perform high-pressure homogenization and cell disruption at 800 bar. Repeat the high-pressure homogenization and cell disruption operation 3 times.

[0070] (3) Take the mixed solution from step 2, stir it at 70°C for 1.0 h, let it stand overnight, take the ethanol layer, and remove the aqueous phase layer.

[0071] (4) The filtrate was filtered using a 0.2 μm CT-300 stacked filter, and the collected filtrate was concentrated under reduced pressure to a paste state at a vacuum degree of 0.08 MPa and a concentration temperature of 65°C. The filtrate was then redissolved with 4 times the weight of water and spray-dried to obtain a light brown powdery Stephania tetrandra extract.

[0072] To verify the activity of the Stephania tetrandra extract provided in this application, the contents of each component of the Stephania tetrandra extract are given below.

[0073] I. Determination of total tetrandrine content in different samples using high performance liquid chromatography (HPLC)

[0074] Specific method: High-performance liquid chromatography (HPLC) was used to determine the content of tetrandrine and tebufenozide (collectively referred to as tetrandrine in this invention) in different processes. A standard sample was placed in a 10 ml volumetric flask, dissolved in methanol, sonicated for 10 min, and diluted to volume to prepare a mother liquor (tebufenozide 1 mg / ml, tebufenozide 0.5 mg / mL). The mobile phase was methanol:0.05% triethylamine aqueous solution (85:15). A C18 column was used with a column temperature of 40℃, a flow rate of 0.8 ml / min, an injection volume of 1 ml, and a detection wavelength of 282 nm. A standard curve was plotted, as shown below. Figures 1-2 The sample peak area was substituted into the formula for calculation, and the results are shown in Table 1.

[0075] Table 1

[0076]

[0077] As shown in Table 1, Examples 1-3 of this application, employing high-pressure homogenization and salting-out extraction techniques, significantly improved the extraction efficiency of tetrandrine, with its content exceeding 62%, significantly superior to the comparative examples. In particular, Comparative Example 1, without high-pressure homogenization, showed a tetrandrine content of 16.8%, and Comparative Example 2, without salting-out, showed a tetrandrine content of 14.3%. Comparative Example 3, using disodium hydrogen phosphate, achieved a tetrandrine content of 45.6%, lower than the examples but higher than the other comparative examples, indicating that the type of salting-out agent significantly affects the extraction effect. Comparative Example 4, using potassium dihydrogen phosphate and without post-treatment with neutral alumina, had a tetrandrine content of only 36.5%, demonstrating the crucial role of post-treatment in extract purity. Comparative Example 5, while employing high-pressure homogenization and ammonium sulfate salting-out, did not use activated clay and neutral alumina for post-treatment, resulting in a tetrandrine content of only 25.3%, further confirming the importance of post-treatment for enriching the active ingredient.

[0078] In summary, the technical solution provided in this application alters the material distribution of the extract through high-pressure homogenization and salting-out extraction, thereby enhancing the removal of impurities such as chlorophyll from the extract by activated clay. Simultaneously, the combination with neutral alumina can chelate polar impurities, thus achieving the retention and purification of the target substance.

[0079] II. Determination of Flavonoid Content by Ultraviolet Spectrophotometry

[0080] Principle: The flavonoid nucleus contains basic oxygen atoms, which generally carry phenolic hydroxyl groups. These atoms can form yellow complexes with aluminum ions. The addition of sodium nitrite and sodium hydroxide makes the solution red in alkaline solution. The solution has maximum absorption at 510 nm. Using rutin as a reference, the absorbance is plotted as linearly with the concentration of rutin. The total flavonoid content is determined by spectrophotometry.

[0081] Procedure: Transfer 0 mL, 1 mL, 2 mL, 3 mL, 4 mL, 5 mL, and 6 mL (0.5 mg / mL) of the standard stock solution into 25 mL volumetric flasks, respectively. Add 6 mL, 5 mL, 4 mL, 3 mL, 2 mL, 1 mL, and 0 mL of water, respectively. Then add 1 mL of 5% NaNO₂ solution to each flask, shake to mix, and let stand for 6 min. Next, add 1 mL of 10% Al(NO₃)₃ solution, shake to mix, and let stand for 6 min. Then add 10 mL of 4% NaOH solution, and dilute to 25 mL with water, shake to mix, and let stand for 15 min. Zero the volume with the corresponding solvent blank, and measure the absorbance at 510 nm. Plot a standard curve with rutin concentration on the x-axis and absorbance at 510 nm on the y-axis, as shown below. Figure 3 The results are shown in Table 2.

[0082] Table 2

[0083]

[0084] Conclusion: Among different sample processing methods, high-pressure homogenization and salt extraction yielded the best results. Comparative Example 3 shows that both methods need to be used in combination to significantly improve the effect; neither can be omitted. High-pressure homogenization and salt extraction alter the material distribution of the extract, causing free proteins to denature and precipitate. Activated clay enhances the removal of impurities such as chlorophyll from the extract. Simultaneously, neutral alumina adsorbs highly polar impurities, thereby achieving the retention and purification of the target analyte.

[0085] III. Chicken Embryo De-stimulation Model

[0086] A 0.05wt% SDS (sodium dodecyl sulfate) stimulation model was established. The IS value and irritation results of SDS at a concentration of 0.05wt% were observed. The changes after mixing with the sample and applying to the CAM for 5 minutes were also recorded. Using SDS (sodium dodecyl sulfate) to establish a stimulation model, the soothing material was mixed with SDS and added to the CAM. The effect of the material antagonizing SDS stimulation was evaluated by observing changes in blood vessels. This was used to evaluate the soothing stimulation efficacy of the 1wt% added sample. The results are shown in Table 3.

[0087] Table 3

[0088]

[0089] Conclusion: The technology of this invention can enhance the de-irritation and soothing effects of Stephania tetrandra extract on a chicken embryo stimulation model. In particular, high-pressure homogenization and salting-out extraction can improve the de-irritation ability and exhibit good repair and antagonistic effects against the ablation and rupture of small blood vessels caused by 0.05% SDS. Changes in blood vessels after 5 minutes in the chicken embryo de-irritation experiment with 0.05% SDS are shown below. Figure 4 Example 1 shows the changes in blood vessels 5 minutes after a chicken embryo destimulation experiment, such as... Figure 5 It is evident that Stephania tetrandra extract can effectively counteract the irritating effect of SDS on chicken embryos.

[0090] IV. Anti-inflammatory experiments using cellular inflammatory factors

[0091] A pro-inflammatory cytokine NO assay was used to establish an inflammation model in RAW264.7 cells induced by lipopolysaccharide (LPS). Well-grown RAW264.7 cells were stimulated with PPS (1 μg / mL) to establish the cellular inflammation model. Changes in NO (nitric oxide) in the cell supernatant were detected by enzyme-linked immunosorbent assay (ELISA). The sample concentration was 0.01 wt%.

[0092] See results Figure 6This invention can enhance the inhibitory rate of Stephania tetrandra extract on the inflammatory factor NO. NO is a key mediator in the acne inflammation process, playing a positive role in antibacterial and immunomodulatory processes in the early stages of inflammation. During periods of uncontrolled inflammation, excessive NO can exacerbate inflammation, leading to tissue damage, pimple redness, pain, and scarring. Regulating NO levels to control the inflammatory response within a moderate and beneficial range can reduce inflammation and decrease acne formation. In this invention, high-pressure homogenization and salting-out extraction can enhance the inhibitory capacity against lipopolysaccharide (LPS)-induced NO in RAW264.7 cells. Especially in Example 1, the process combining adsorbent and decolorizing agent can increase the inhibitory capacity by approximately four times.

[0093] V. Acne-removing efficacy (for trial use by a small group of people)

[0094] A healthy population aged 18-40 with oily skin, more than 3 blackheads on the face, a total number of pimples greater than 3, and no dense acne, acne marks, or acne pits (N=28) was selected. A serum containing 0.5wt% of the Stephania tetrandra extract from Example 1 (without other active ingredients) was used. The serum was applied by spotting three times a day. Skin condition was reviewed on days 0, 1, 3, and 7. The results were obtained by counting skin lesions and scoring using ISGA. The results are shown in Table 4.

[0095] Table 4

[0096]

[0097] Conclusion: As shown in Table 4, the serum containing 0.5 wt% of the Stephania tetrandra extract from Example 1 had a significant acne-reducing effect on day 7 of the experiment. Specifically, the number of facial comedones decreased by 56.25% compared to baseline (D0), the number of facial papules and pustules decreased by 42.90%, and the total number of facial lesions (comedones + papules + pustules + nodules + cysts) decreased by 58.66%. Simultaneously, the facial ISGA score, reflecting the overall improvement in lesions, also decreased by 45.38%. Notably, the improvement in the total number of comedones and lesions was highly statistically significant from day 3 (**p<0.01), and the significant improvements in the number of papules and pustules and the ISGA score also reached statistical significance on days 3 and 7, respectively (*p<0.05 and **p<0.01). This indicates that the Stephania tetrandra extract can effectively improve acne-related non-inflammatory (comedones) and inflammatory (papules, pustules) skin lesions, and the effect tends to increase with the time of use. This proves that the highly active Stephania tetrandra extract provided in this application can significantly and effectively improve the number of facial comedones, facial papules, pustules and other symptoms, and significantly improve the overall skin condition.

Claims

1. A method for preparing a high-activity powder extract of Stephania tetrandra, characterized in that, The method comprises the following steps: 1) crushing and sieving the powder of Stephania tetrandra S. Moore, adding ethanol aqueous solution according to the solid-liquid ratio of 1:20-30, soaking for 10-20 min, and then adding salt accounting for 15-35% of the weight of the solution and stirring uniformly; 2) starting the condensation circulation device, controlling the temperature at 8-10 ℃, and performing high-pressure homogenization under the pressure of 400-1000 bar; 3) taking the mixed solution obtained in step 2), adding activated bleaching earth accounting for 1-5% of the weight of the mixed solution at the temperature of 60-80 ℃, stirring for 0.5-2 h, and then standing overnight, taking the ethanol layer, and removing the water phase layer; 4) adding neutral alumina accounting for 0.5-3% of the weight of the ethanol layer at the temperature of 25-60 ℃, and stirring for 0.5-1.0 h; 5) using a 0.2 μm stacked filter to concentrate the filtrate to extract, redissolving with water, and then performing spray drying to obtain a light yellow-light brown powder of Stephania tetrandra S. Moore extract.

2. The method for preparing the highly active powdered Stephania tetrandra extract according to claim 1, characterized in that, The salt is one or a combination of phosphoric acid salt and sulfuric acid salt.

3. The method for preparing the highly active powdered Stephania tetrandra extract according to claim 1, characterized in that, The sulfuric acid salt is ammonium sulfate or potassium sulfate.

4. The method for preparing the highly active powdered Stephania tetrandra extract according to claim 1, characterized in that, The ethanol aqueous solution is an ethanol aqueous solution with the volume fraction of ethanol of 10-50%.

5. The method for preparing the highly active powdered Stephania tetrandra extract according to claim 1, characterized in that, The high-pressure homogenization time is 2-3 min.

6. The method for preparing the highly active powdered Stephania tetrandra extract according to claim 1, characterized in that, The high-pressure homogenization is repeated for 2-4 times.

7. The method for preparing the highly active powdered Stephania tetrandra extract according to claim 1, characterized in that, The pore size of the filter membrane used in the filtration is 0.2 μm.

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