Corn embryo extraction method for optimizing collection efficiency

Abstract

The invention relates to the field of embryo extract extraction, in particular to a corn embryo extract extraction method for optimizing collection efficiency, which comprises the following steps: adjusting the water content of corn, and crushing by using a fluted disc crusher; the crushing qualification is judged according to the crushing comprehensive efficiency index; determining an unqualified reason according to the corn crushing rate under the condition of unqualified crushing; separating germs from the qualified crushed corn by adopting a cyclone separator; judging the qualification of germ separation according to the germ purity characteristic value; under the condition of unqualified separation, determining the reason of unqualified separation according to the proportion of the abnormal germs; cleaning and drying the qualified separated germs, and adding a compound enzyme for enzymolysis; performing ultrasonic treatment on the mixture subjected to enzymolysis, and performing centrifugal separation to obtain supernatant liquid; adsorbing the supernatant liquid through macroporous adsorption resin, and carrying out gradient elution by adopting ethanol; and carrying out vacuum concentration on the eluent, and freeze-drying to obtain the corn embryo extract. According to the invention, the collection efficiency of the corn embryo extract is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of embryo extract, and particularly relates to a corn embryo extract method for optimizing collection efficiency. BACKGROUND

[0002] Corn embryo extract is a natural ingredient with important nutritional value and biological activity extracted from corn germ, which mainly contains unsaturated fatty acids (linoleic acid and oleic acid content of 50-60%), vitamin E (mainly alpha-tocopherol, content of about 0.1-0.3%), phytosterols (beta-sitosterol, stigmasterol, etc.) and various antioxidants. These active ingredients endow corn embryo extract with significant physiological functions such as antioxidant, hypolipidemic, anti-inflammatory, etc., making it have wide application prospects in functional foods, pharmaceutical preparations and high-end cosmetics, etc. fields. The traditional germ separation technology has problems of low separation efficiency and poor germ integrity, resulting in high impurity content in the subsequent extract.

[0003] Chinese patent publication No. CN106820143B discloses a plant embryo extract extraction method, comprising the following steps: (1) obtaining of germ: selecting high-quality seeds, washing 3-10 times, then soaking in hot water for 3-10 min, quickly changing cold water for 4-10 h, taking out the seeds, germinating on wet gauze; after germination, low-temperature treatment for 4-15 h, continue to germinate to obtain germ raw material; (2) raw material pretreatment: taking 5.0 g of plant embryo, washing 2-4 times with water, adding 20-50 mL of water, and crushing; (3) ultrasonic-assisted water extraction: placing the crushed liquid raw material in an ultrasonic experimental device for extraction; (4) filtration separation and purification: taking the clear liquid first filters out the solid phase with a filter screen, then filters with a fiber ultrafiltration membrane after centrifugation, and the obtained filtrate contains plant embryo extract. The plant embryo extract extraction method of the present application is simple, fast, efficient, inexpensive and highly applicable, and the whole process is mild extraction without high temperature and high pressure conditions, which can well preserve the biological activity of the plant embryo extract.

[0004] However, the prior art has the following problems: the prior art lacks detection and regulation during the separation process of raw materials, resulting in low germ separation efficiency and poor integrity. SUMMARY

[0005] Therefore, the present application provides a corn embryo extract method for optimizing collection efficiency to overcome the problem of low germ separation efficiency and poor quality caused by the lack of detection and regulation in the separation process in the prior art.

[0006] To achieve the above-mentioned purpose, the present application provides a corn embryo extract method for optimizing collection efficiency, comprising:

[0007] The corn after impurity removal is adjusted for water content by a wetting system and then crushed by a toothed disc crusher;

[0008] According to the crushing comprehensive performance index obtained by sampling detection, the crushing qualification is determined, and under the condition of unqualified crushing, the tooth disc spacing is increased, or according to the corn crushing rate, the unqualified reason is determined and the water content of corn is reduced or the rotating speed of the tooth disc crusher is reduced;

[0009] The germ is separated from the qualified corn crushing by adopting a cyclone separator;

[0010] According to the germ purity characteristic value obtained by image detection, the germ separation qualification is determined, and under the condition of unqualified separation, the inlet pressure of the cyclone separator is increased, or according to the proportion of abnormal germs, the unqualified separation reason is determined and the abnormal germs are screened out or the rotating speed of the cyclone separator is increased;

[0011] The separated germ is washed and dried, and then added to a composite enzyme for enzymolysis;

[0012] The mixture after enzymolysis is treated by ultrasonic wave and then centrifuged to separate the supernatant;

[0013] The supernatant is adsorbed by a macroporous adsorption resin, and then gradient eluted by ethanol;

[0014] The eluate is reduced pressure concentrated and then freeze-dried to obtain the corn embryo extract.

[0015] Further, the crushing comprehensive performance index is determined by the germ integrity and the crushing uniformity.

[0016] Further, according to the crushing comprehensive performance index, the crushing qualification is determined, wherein if the crushing comprehensive performance index is less than a first preset index, the crushing is determined to be unqualified, and the tooth disc spacing of the tooth disc crusher is increased according to the difference between the first preset index and the crushing comprehensive performance index.

[0017] If the crushing comprehensive performance index is greater than or equal to the first preset index and less than a second preset index, the crushing is determined to be unqualified, and the unqualified reason is determined according to the corn crushing rate.

[0018] If the crushing comprehensive performance index is greater than or equal to the second preset index, the crushing is determined to be qualified, and the germ is separated by adopting a cyclone separator.

[0019] Further, the tooth disc spacing is positively correlated with the performance index difference, wherein the performance index difference is the difference between the first preset index and the crushing comprehensive performance index.

[0020] Further, the reason for the unqualified crushing is determined according to the corn crushing rate, wherein if the corn crushing rate is less than a preset crushing rate, it is determined that the unqualified reason is that the corn moisture is too high, and the water content of the corn is reduced according to the difference between the preset crushing rate and the corn crushing rate.

[0021] If the corn crushing rate is greater than or equal to the preset crushing rate, it is determined that the unqualified reason is that the tooth disc crusher rotation speed is too fast, and the rotation speed of the tooth disc crusher is reduced according to the difference between the corn crushing rate and the preset crushing rate.

[0022] Further, there are several adjustment modes for the rotation speed of the tooth disc crusher, and each adjustment mode has a different adjustment range for the rotation speed.

[0023] Further, the quality of the germ separation is determined according to the germ purity characteristic value, wherein if the germ purity characteristic value is less than a first preset characteristic value, it is determined that the germ separation is unqualified, and the inlet pressure of the cyclone separator is increased according to the difference between the first preset characteristic value and the germ purity characteristic value.

[0024] If the germ purity characteristic value is greater than or equal to the first preset characteristic value and less than a second preset characteristic value, it is determined that the germ separation is unqualified, and the reason for the unqualified separation is determined according to the abnormal germ proportion.

[0025] If the germ purity characteristic value is greater than or equal to the second preset characteristic value, it is determined that the germ separation is qualified, and the germ is cleaned and dried before being enzymatically hydrolyzed.

[0026] Further, the germ purity characteristic value is determined by the germ color uniformity and the germ contour integrity.

[0027] Further, the inlet pressure of the cyclone separator is positively correlated with the germ purity difference, and the germ purity difference is the difference between the first preset characteristic value and the germ purity characteristic value.

[0028] Further, the reason for the unqualified separation is determined according to the abnormal germ proportion, wherein if the abnormal germ proportion is less than a preset germ proportion, it is determined that the unqualified reason is that the quality of the corn raw material is problematic, and the abnormal germ is screened out by an automatic screening device.

[0029] If the abnormal germ proportion is greater than or equal to the preset germ proportion, it is determined that the unqualified reason for the separation is that the rotation speed of the cyclone separator is not up to standard, and the rotation speed of the cyclone separator is increased according to the difference between the abnormal germ proportion and the preset germ proportion.

[0030] Compared with the prior art, the present application has the beneficial effects that the present application obtains a crushing comprehensive performance index through sampling detection, which is determined by the embryo integrity and crushing uniformity, and is used to determine the qualification of crushing, when the crushing is unqualified, the tooth disc spacing of the tooth disc crusher is increased, or the unqualified reason is determined according to the corn crushing rate, the detection precision is improved by combining the crushing quality detection with the dynamic parameter adjustment, and the integrity of embryo separation is ensured.

[0031] Further, the present application determines the unqualified reason according to the corn crushing rate, including that the corn moisture is too much or the tooth disc crusher rotating speed is too fast, the existing problems are solved by reducing the water content of the corn or reducing the rotating speed of the tooth disc crusher, and the efficiency and quality of corn crushing are improved.

[0032] Further, the present application determines the unqualified reason according to the corn crushing rate, including that the corn moisture is too much or the tooth disc crusher rotating speed is too fast, the existing problems are solved by reducing the water content of the corn or reducing the rotating speed of the tooth disc crusher, and the efficiency and quality of corn crushing are improved.

[0033] Further, the present application determines the unqualified reason according to the corn crushing rate, including that the corn moisture is too much or the tooth disc crusher rotating speed is too fast, the existing problems are solved by reducing the water content of the corn or reducing the rotating speed of the tooth disc crusher, and the efficiency and quality of corn crushing are improved. BRIEF DESCRIPTION OF DRAWINGS

[0034] Fig. 1 The flow chart of the corn embryo element extraction method for optimizing the collection efficiency of the embodiment of the present application;

[0035] Fig. 2 The flow chart of the embodiment of the present application for determining the qualification of crushing according to the crushing comprehensive performance index;

[0036] Fig. 3 The flow chart of the embodiment of the present application for determining the unqualified reason of crushing according to the corn crushing rate;

[0037] Fig. 4 The flow chart of the embodiment of the present application for determining the qualification of embryo separation according to the embryo purity characteristic value. DETAILED DESCRIPTION

[0038] In order to make the purpose and advantages of the present application more clear and explicit, the present application is further described below in combination with embodiments; it should be understood that the specific embodiments described herein are only used to explain the present application, and do not limit the present application.

[0039] The preferred embodiments of the present application are described below with reference to the accompanying drawings. Those skilled in the art will understand that the embodiments are only used to explain the technical principles of the present application and are not intended to limit the protection scope of the present application.

[0040] It should be noted that the data in the present embodiment are obtained by comprehensive analysis and evaluation of historical detection data and corresponding historical detection results in the three months before the present detection according to the present application. Those skilled in the art can understand that the determination method of the present application for a single parameter can be to select the value with the highest proportion as the preset standard parameter according to the data distribution, to use weighted summation to obtain the value as the preset standard parameter, to substitute each historical data into a specific formula and to obtain the value by using the formula as the preset standard parameter, or other selection methods, as long as the present application can clearly define different specific conditions in the single determination process by the obtained value.

[0041] Please refer to Figs. 1 to 4 respectively, the flowchart of the corn embryo element extraction method for optimizing collection efficiency according to the embodiments of the present application; the flowchart of determining the qualification of crushing according to the crushing comprehensive performance index according to the embodiments of the present application; the flowchart of determining the reason for unqualified crushing according to the corn crushing rate according to the embodiments of the present application; and the flowchart of determining the qualification of embryo separation according to the embryo purity characteristic value according to the embodiments of the present application.

[0042] The corn embryo element extraction method for optimizing collection efficiency according to the embodiments of the present application comprises:

[0043] Step S1, the impurity-removed corn is adjusted by a wetting system to adjust the water content, and then is crushed by a toothed disc crusher;

[0044] Step S2, the qualification of crushing is determined according to the crushing comprehensive performance index obtained by sampling detection, the toothed disc spacing is increased under the condition of unqualified crushing, or the reason for unqualified crushing is determined according to the corn crushing rate, and the water content of the corn or the rotating speed of the toothed disc crusher is reduced;

[0045] Step S3, the embryo is separated from the qualified corn by a cyclone separator;

[0046] Step S4, the qualification of embryo separation is determined according to the embryo purity characteristic value obtained by image detection, the inlet pressure of the cyclone separator is increased under the condition of unqualified separation, or the reason for unqualified separation is determined according to the proportion of abnormal embryos, and the abnormal embryos are screened out or the rotating speed of the cyclone separator is increased;

[0047] Step S5, the separated and qualified embryo is washed and dried, and then is added with a composite enzyme for enzymolysis;

[0048] Step S6, the mixture after enzymolysis is treated by ultrasonic waves, and then is centrifuged to separate the supernatant.

[0049] Step S7, after the supernatant is adsorbed by macroporous adsorption resin, gradient elution is performed using ethanol;

[0050] Step S8, after the eluate is concentrated under reduced pressure, freeze-drying is performed to obtain zeatin.

[0051] Specifically, the wetting system is composed of a spraying device, a humidity sensor, a circulating fan and a PLC control system. The moisture content of the corn is dynamically adjusted by uniformly spraying water mist or steam, and the initial moisture content of the corn is set to 18%.

[0052] Specifically, the initial tooth disc spacing of the tooth disc crusher is set to 1 mm, and the initial rotating speed is set to 600 rpm.

[0053] Specifically, the sampling detection is to randomly take 100g sample from the crushed corn mixture, and the comparison between the embryo obtained after crushing and the embryo in the uncrushed corn is carried out by image detection combined with AI algorithm. The crushed embryo with similarity greater than 90% after comparison is recorded as complete embryo, and the ratio of complete embryo to all embryos obtained after crushing is recorded as embryo integrity. The crushing uniformity is the ratio of the number of particles in the target interval (0.5-2mm) to the total number of particles.

[0054] Specifically, the initial inlet pressure of the cyclone separator is set to 0.2MPa, and the initial rotating speed is set to 3000rpm.

[0055] Specifically, the crushing comprehensive performance index is determined by embryo integrity and crushing uniformity, and is calculated by the following formula, In the formula, E is the crushing comprehensive performance index, α is the first weight coefficient, α is set to 0.6, C is the embryo integrity, Cy is the embryo integrity threshold, β is the second weight coefficient, β is set to 0.4, U is the crushing uniformity, Uy is the crushing uniformity threshold, and Uy is set to 85%.

[0056] Specifically, the crushing is determined to be qualified according to the crushing comprehensive performance index. If the crushing comprehensive performance index is less than the first preset index 0.75, the crushing is determined to be unqualified, and the tooth disc spacing of the tooth disc crusher is increased according to the difference between the first preset index and the crushing comprehensive performance index.

[0057] If the crushing comprehensive performance index is greater than or equal to the first preset index and less than the second preset index 0.86, the crushing is determined to be unqualified, and the reason for unqualification is determined according to the corn crushing rate.

[0058] If the crushing comprehensive performance index is greater than or equal to the second preset index, the crushing is determined to be qualified, and the embryo is separated by the cyclone separator.

[0059] In this embodiment of the invention, the first preset index is 0.75 and the second preset index is 0.86. However, the above values ​​are not limited to these values, and those skilled in the art can adjust the above values ​​according to actual needs.

[0060] Specifically, the gear spacing is positively correlated with the performance index difference. If the performance index difference is less than the first preset index difference of 0.04, the gear spacing is increased to the corresponding value using the first spacing adjustment coefficient of 1.2.

[0061] If the performance index difference is greater than or equal to the first preset index difference and less than the second preset index difference of 0.09, then the toothed disc pitch is increased to the corresponding value using the second pitch adjustment coefficient of 1.5.

[0062] If the performance index difference is greater than or equal to the second preset index difference, then the toothed disc pitch is increased to the corresponding value using the third pitch adjustment coefficient 2.0;

[0063] The efficiency index difference is the difference between the first preset index and the comprehensive crushing efficiency index.

[0064] In this embodiment of the invention, the first preset index difference is 0.04 and the second preset index difference is 0.09. However, the above values ​​are not limited to these values, and those skilled in the art can adjust the above values ​​according to actual needs.

[0065] Specifically, the reason for the failure to meet the breakage standard is determined based on the corn breakage rate. If the corn breakage rate is less than the preset breakage rate, the reason for the failure is determined to be excessive moisture in the corn, and the moisture content of the corn is reduced based on the difference between the preset breakage rate and the corn breakage rate.

[0066] If the corn breakage rate is greater than or equal to the preset breakage rate, the reason for non-compliance is determined to be that the toothed disc crusher rotates too fast, and the rotation speed of the toothed disc crusher is reduced according to the difference between the corn breakage rate and the preset breakage rate.

[0067] Specifically, the moisture content of corn is negatively correlated with the first breakage rate difference, where the first breakage rate difference is the difference between the preset breakage rate and the corn breakage rate, and the corn moisture content is adjusted within the range of 12-18%.

[0068] Specifically, there are several adjustment methods for the rotational speed of the toothed disc crusher, and each adjustment method has a different adjustment range for the rotational speed. The rotational speed of the toothed disc crusher is negatively correlated with the second crushing rate difference. The rotational speed adjustment range of the toothed disc crusher is 400-600 rpm. The second crushing rate difference is the difference between the corn crushing rate and the preset crushing rate.

[0069] Specifically, the qualification of germ separation is determined based on the germ purity characteristic value. If the germ purity characteristic value is less than the first preset characteristic value of 0.80, the germ separation is deemed unqualified, and the inlet pressure of the cyclone separator is increased based on the difference between the first preset characteristic value and the germ purity characteristic value.

[0070] If the germ purity characteristic value is greater than or equal to the first preset characteristic value and less than the second preset characteristic value of 0.92, the germ separation is determined to be unqualified, and the reason for the unqualified separation is determined according to the proportion of abnormal germs.

[0071] If the germ purity characteristic value is greater than or equal to the second preset characteristic value, the germ separation is deemed qualified, and the germ is washed, dried, and then enzymatically hydrolyzed.

[0072] In this embodiment of the invention, the first preset feature value is 0.80 and the second preset feature value is 0.92. However, the above values ​​are not limited to these values, and those skilled in the art can adjust the above values ​​according to actual needs.

[0073] Specifically, the germ purity characteristic value is determined by both germ color uniformity and germ outline integrity, and is calculated using the following formula: In the formula, P is the germ purity feature value, ω1 is the third weighting coefficient, R is the germ color uniformity, Ry is the germ color uniformity threshold, set Ry = 0.9, ω2 is the fourth weighting coefficient, set ω1 = ω2 = 0.5, S is the germ outline integrity, Sy is the germ outline integrity threshold, set Sy = 0.8.

[0074] Specifically, the uniformity of germ color is obtained by taking pictures of germ samples with a high-resolution RGB camera, obtaining the proportion of abnormal color pixels, and then comparing it with the total germ pixels. The uniformity of germ color is calculated as 1 - the proportion of abnormal color pixels / the total germ pixels. The integrity of germ outline is obtained by segmenting the germ outline using the Canny algorithm.

[0075] Specifically, the inlet pressure of the cyclone separator is positively correlated with the difference in germ purity. The adjustment range of the inlet pressure is 0.2-0.5 MPa, and the difference in germ purity is the difference between the first preset characteristic value and the germ purity characteristic value.

[0076] Specifically, the reason for the separation failure is determined based on the percentage of abnormal germs. If the percentage of abnormal germs is less than 5% of the preset percentage of germs, the reason for the separation failure is determined to be a quality problem with the corn raw material, and the abnormal germs are removed by automated screening equipment.

[0077] If the percentage of abnormal germs is greater than or equal to the preset percentage of germs, the reason for the separation failure is determined to be that the speed of the hydrocyclone separator is not up to standard, and the speed of the hydrocyclone separator is increased according to the difference between the percentage of abnormal germs and the preset percentage of germs.

[0078] Specifically, abnormal embryos include abnormal color, incomplete embryo structure, and shriveled texture, etc., without being limited to any specific type. The percentage of abnormal embryos is obtained through image detection.

[0079] In this embodiment of the invention, the preset embryo ratio is 5%, but the above value is not limited to this. Those skilled in the art can adjust the above value according to actual needs.

[0080] Specifically, the rotational speed of the hydrocyclone separator is positively correlated with the difference in the proportion of germs, wherein the difference in the proportion of germs is the difference between the abnormal germ proportion and the preset germ proportion, and the rotational speed of the hydrocyclone separator is adjusted within the range of 3000-5000 rpm.

[0081] The technical solution of the present invention has been described above with reference to the preferred embodiments shown in the accompanying drawings. However, it will be readily understood by those skilled in the art that the scope of protection of the present invention is obviously not limited to these specific embodiments. Without departing from the principles of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will all fall within the scope of protection of the present invention.

[0082] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for extracting corn embryonic stem cells to optimize collection efficiency, characterized in that, include: After removing impurities, the corn is conditioned by a wetting system and then crushed by a toothed disc crusher. The passability of crushing is determined by the comprehensive crushing efficiency index obtained from sampling inspection. If the crushing is not qualified, the tooth disc spacing is increased, or the cause of the failure is determined by the corn crushing rate and the moisture content of the corn is reduced or the speed of the tooth disc crusher is reduced. The germ is separated from crushed and qualified corn using a hydrocyclone separator; The qualification of germ separation is determined by the germ purity characteristic value obtained by image detection. If the separation is unqualified, the inlet pressure of the hydrocyclone is increased, or the reason for the unqualified separation is determined by the proportion of abnormal germs and abnormal germs are screened out or the speed of the hydrocyclone is increased. After the successfully separated embryos are cleaned and dried, they are added to a compound enzyme for enzymatic hydrolysis. The mixture after enzymatic hydrolysis was treated with ultrasound and then centrifuged to separate the supernatant. After the supernatant was adsorbed through a macroporous adsorption resin, it was eluted with ethanol in a gradient. The eluent was concentrated under reduced pressure and then freeze-dried to obtain corn embryonic extract.

2. The method for extracting maize embryonic stem cells to optimize collection efficiency according to claim 1, characterized in that, The overall crushing efficiency index is determined by both germ integrity and crushing uniformity.

3. The method for extracting maize embryonic stem cells to optimize collection efficiency according to claim 1, characterized in that, The passability of crushing is determined based on the comprehensive crushing efficiency index. If the comprehensive crushing efficiency index is less than a first preset index, the crushing is deemed unqualified, and the tooth spacing of the toothed disc crusher is increased based on the difference between the first preset index and the comprehensive crushing efficiency index. If the comprehensive crushing efficiency index is greater than or equal to the first preset index and less than the second preset index, the crushing is deemed unqualified, and the reason for the unqualification is determined based on the corn crushing rate. If the overall crushing efficiency index is greater than or equal to the second preset index, the crushing is deemed qualified, and the germ is separated using a cyclone separator.

4. The method for extracting corn embryonic stem cells to optimize collection efficiency according to claim 3, characterized in that, The tooth disc spacing is positively correlated with the efficiency index difference, wherein the efficiency index difference is the difference between the first preset index and the crushing comprehensive efficiency index.

5. The method for extracting corn embryonic stem cells to optimize collection efficiency according to claim 3, characterized in that, The reason for the failure to meet the breakage standard is determined based on the corn breakage rate. If the corn breakage rate is less than the preset breakage rate, the reason for the failure is determined to be that the corn has too much moisture. The moisture content of the corn is then reduced based on the difference between the preset breakage rate and the corn breakage rate. If the corn breakage rate is greater than or equal to the preset breakage rate, the reason for non-compliance is determined to be that the toothed disc crusher rotates too fast, and the rotation speed of the toothed disc crusher is reduced according to the difference between the corn breakage rate and the preset breakage rate.

6. The method for extracting corn embryonic stem cells for optimizing collection efficiency according to claim 5, characterized in that, There are several adjustment methods for the rotational speed of the toothed disc crusher, and each adjustment method has a different adjustment range for the rotational speed.

7. The method for extracting maize embryonic stem cells to optimize collection efficiency according to claim 1, characterized in that, The qualification of germ separation is determined based on the germ purity characteristic value. If the germ purity characteristic value is less than a first preset characteristic value, the germ separation is deemed unqualified, and the inlet pressure of the cyclone separator is increased according to the difference between the first preset characteristic value and the germ purity characteristic value. If the germ purity characteristic value is greater than or equal to the first preset characteristic value and less than the second preset characteristic value, then the germ separation is determined to be unqualified, and the reason for the unqualified separation is determined according to the proportion of abnormal germs. If the germ purity characteristic value is greater than or equal to the second preset characteristic value, the germ separation is deemed qualified, and the germ is washed, dried, and then enzymatically hydrolyzed.

8. The method for extracting maize embryonic stem cells for optimizing collection efficiency according to claim 7, characterized in that, The germ purity characteristic value is determined by both the uniformity of germ color and the integrity of germ outline.

9. The method for extracting maize embryonic stem cells for optimizing collection efficiency according to claim 7, characterized in that, The inlet pressure of the hydrocyclone separator is positively correlated with the difference in germ purity, which is the difference between the first preset characteristic value and the germ purity characteristic value.

10. The method for extracting maize embryonic stem cells for optimizing collection efficiency according to claim 7, characterized in that, The reason for the separation failure is determined based on the percentage of abnormal germs. If the percentage of abnormal germs is less than the preset percentage of germs, the reason for the separation failure is determined to be a quality problem with the corn raw material, and the abnormal germs are removed by automated screening equipment. If the percentage of abnormal germs is greater than or equal to the preset percentage of germs, the reason for the separation failure is determined to be that the speed of the hydrocyclone separator is not up to standard, and the speed of the hydrocyclone separator is increased according to the difference between the percentage of abnormal germs and the preset percentage of germs.

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