A pretreatment device for detecting escherichia coli

Abstract

The present application relates to the technical field of microbial detection automation equipment, and discloses a pretreatment equipment for escherichia coli detection, which solves the problems proposed in the background art, and comprises a base, a filling station and a rotary driving assembly arranged on the base. The driving assembly drives the L-shaped workbench to rotate between the initial station and the processing station through the sleeve. The sleeve is provided with a bag opening clamping mechanism on the side, and the elastic adaptive module is used to fix the bag opening, which can effectively buffer the tension when the bag opening is folded. The horizontal part of the L-shaped workbench is provided with a bag body processing mechanism, which comprises a sealing clamp driven by a bidirectional screw and provided with a sealing convex rib and a groove, and an inertia beating assembly driven by a cylinder and capable of reciprocating. The beating assembly realizes soft beating through the inertia of the flexible beating plate. The present application simulates the "holding bag" and "beating" actions of human hands through the automatic mechanism, realizes the automatic filling, sealing and efficient beating mixing of the homogeneous bag, and improves the automation degree and processing efficiency of the pretreatment.

Description

Technical Field

[0001] This invention relates to the field of automated equipment for microbial detection, specifically a pretreatment device for detecting Escherichia coli. Background Technology

[0002] In the field of microbial detection, especially for the detection of Escherichia coli, sample pretreatment is a crucial step in determining the accuracy and reliability of test results. This process typically involves placing the sample to be tested with sterile diluent or culture medium in a dedicated homogenization bag for thorough mixing and homogenization, ensuring that the microorganisms are evenly distributed in the liquid.

[0003] Currently, this pretreatment process is highly dependent on manual operation: after opening the homogenization bag, the operator pours in the sample and liquid, then manually seals or clamps the bag opening, and then repeatedly pats, kneads or shakes the homogenization bag to achieve sample homogenization. This manual operation mode is inefficient, takes a long time to process a single sample, and is difficult to meet the growing high-throughput demands of modern testing laboratories. Summary of the Invention

[0004] To address the aforementioned problems, the present invention provides the following technical solution: a pretreatment device for Escherichia coli detection, comprising a base, on which a filling station is provided. A rotary drive assembly is mounted on the base, and a sleeve is fixedly connected to the output end of the rotary drive assembly. The sleeve is fixedly connected to the vertical part of an L-shaped worktable, and the rotary drive assembly is capable of driving the L-shaped worktable to rotate between an initial station and a processing station.

[0005] A bag mouth clamping mechanism is connected to one side of the sleeve. The bag mouth clamping mechanism is located above the vertical part of the L-shaped workbench and is used to fix the bag mouth of the homogenizing bag at the initial work position.

[0006] A bag body processing mechanism is provided on the horizontal part of the L-shaped workbench. The bag body processing mechanism includes a sealing clamp for pressing the bag body and an inertial beating component for beating the bag body.

[0007] The device also includes a control unit, which is electrically connected to the rotary drive assembly, the filling station, and the bag handling mechanism.

[0008] When the L-shaped workbench rotates from the initial station to the processing station, the filling station can fill liquid into the homogeneous bag fixed by the bag mouth clamping mechanism. Subsequently, the sealing clamp can press the bag body, and the inertial tapping component can tap the bag body.

[0009] Furthermore, the bag opening clamping mechanism includes a crossbeam fixed to one side of the sleeve and clamping arm assemblies symmetrically arranged at both ends of the crossbeam. The clamping arm assembly includes clamping arms, a bag opening clamp, and an elastic adaptive module. The clamping arm consists of a horizontal section and a downwardly inclined section. The elastic adaptive module is connected between the bag opening clamp and the end of the inclined section of the clamping arm. When the sealing clamp presses against the bag body, causing the bag opening to close, the elastic adaptive module can buffer the pulling force on the bag opening through its own deformation or displacement, preventing the bag opening from being torn or excessively stretched.

[0010] Furthermore, one specific implementation of the elastic adaptive module is as follows: it includes a T-shaped groove horizontally opened on the side of the inclined section of the clamping arm, a slidable T-shaped slider is adaptedly installed in the T-shaped groove, a connecting ring is fixedly connected to the T-shaped slider, and the connecting ring is connected to the bag opening clip through a tension spring.

[0011] Furthermore, the sealing clamp includes a bidirectional lead screw rotatably mounted on the L-shaped worktable, which is driven by a second drive motor. Clamping jaws are respectively engaged on the two reverse threads of the bidirectional lead screw. Sealing ribs made of elastic material and sealing grooves adapted to the sealing ribs are respectively provided on the opposing clamping surfaces of the two clamping jaws to achieve reliable sealing and clamping of the bag body.

[0012] Furthermore, a sliding block is provided on the clamping jaw, and a rotating shaft is fixedly connected to the clamping jaw. A torsion spring is provided outside the rotating shaft, and the rotating shaft is rotatably mounted on the sliding block. When the torsion spring is in a free state, the two clamping jaws are arranged in a pattern where one end is close to each other and the other end is far apart. The sliding block is threadedly engaged with the bidirectional lead screw. A pressure block is fixedly connected to the clamping jaw, and the pressure block can engage with the bag opening clamp so that the bag opening clamp bites at one end of the homogenized bag. Mounting grooves are provided on the opposite sides of the two clamping jaws. A movable block is movably mounted in the mounting groove. The movable block is connected to the inner wall of the mounting groove by a spring. A transmission gear is rotatably mounted in the clamping jaw. A first rack is fixedly connected to the movable block, and a second rack is fixedly connected to the pressure block. The first rack and the transmission gear, and the second rack and the transmission gear are all meshed with each other so that when the movable block is pressed, the pressure block can move upward.

[0013] Furthermore, the inertial striking assembly includes a linear guide rail fixed to the horizontal part of the L-shaped worktable, and a sliding seat slidably fitted on the linear guide rail. The sliding seat is driven to reciprocate by a reciprocating drive cylinder fixed to the L-shaped worktable. A flexible striking plate is mounted on the sliding seat via a swing connection structure.

[0014] Furthermore, a preferred structure of the flexible clapper includes a mounting rod with its bottom fixed to the sliding seat, a flexible connecting rod connected to the top of the mounting rod, and a clapper body made of flexible material connected to the end of the flexible connecting rod. This structure can simulate the gentle clapping motion of a human hand, avoiding mechanical damage to the homogenizing bag.

[0015] Furthermore, a position sensor for detecting the position of the sliding seat is provided at one end of the linear guide rail to achieve precise control of the reciprocating motion.

[0016] Furthermore, support pads are provided on the machine base at positions corresponding to the initial work station and the processing work station to support the horizontal part of the L-shaped worktable, so as to enhance the structural rigidity and stability of the equipment under the tapping condition.

[0017] Furthermore, the rotary drive assembly includes a first servo motor fixed to the base, the output shaft of which is coaxially connected to the bottom end of the sleeve via a coupling. An angle sensor electrically connected to the control unit is also provided on the base to detect the rotation angle of the L-shaped worktable, thereby achieving precise positioning of the workstation.

[0018] Furthermore, the control unit is a programmable logic controller, which is connected to the first servo motor in the rotary drive assembly, the metering pump in the filling station, the second drive motor, the reciprocating drive cylinder, the position sensor, and the angle sensor to coordinate the automatic operation of each component according to a preset program.

[0019] Furthermore, the filling station includes a bracket fixed to the machine base, a liquid storage tank mounted on the bracket, a metering pump communicating with the liquid storage tank, and a filling tube connected to the outlet of the metering pump. The outlet end of the filling tube corresponds to the opening position of the homogenized bag fixed by the bag opening clamping mechanism at the processing station. The metering pump is electrically connected to the control unit to achieve precise and automatic liquid filling.

[0020] Compared with the prior art, the beneficial effects of the present invention are:

[0021] Through the coordination of the control unit, the entire process from homogenizing bag rotation and positioning, liquid filling, bag sealing to inertial tapping is fully automated, significantly improving pretreatment efficiency and freeing up manpower. Operators only need to clamp the empty homogenizing bag on the bag mouth clamping mechanism at the initial station, and the subsequent rotation, filling, sealing, tapping, and resetting processes are all automatically controlled by the control unit. The rotating design of the L-shaped worktable separates the loading and unloading station from the processing station, forming a simple production line. This allows operators to prepare the next sample while the equipment processes one sample, greatly reducing human-machine waiting time and improving overall processing efficiency. It is particularly suitable for batch sample testing. Attached Figure Description

[0022] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings:

[0023] Figure 1 This is a three-dimensional schematic diagram of the overall structure of the present invention (at the processing station).

[0024] Figure 2 This is a side view of the structure of the present invention;

[0025] Figure 3 For the present invention Figure 2 A magnified view of a section at point A in the middle;

[0026] Figure 4 This is a schematic diagram of the sealing clamp and inertial tapping assembly of the present invention;

[0027] Figure 5 This is a schematic diagram of the mating structure of the sealing rib and sealing groove of the present invention;

[0028] Figure 6 This is a schematic diagram illustrating the combination of the bag opening clip and the homogenizing bag according to the present invention.

[0029] Figure 7 This is a schematic diagram of the mating structure of the two bag opening clips of the present invention;

[0030] Figure 8 This is a top view schematic diagram of the two clamping jaws engaging in the present invention;

[0031] Figure 9 This is a schematic diagram of the internal structure of the clamp claw of the present invention.

[0032] In the diagram: 1. Base; 2. Sleeve; 3. L-shaped worktable; 4. Sealing clamp; 5. Inertial tapping assembly; 6. Crossbeam; 7. Clamping arm; 8. Bag opening clamp; 9. T-shaped slide rail; 10. T-shaped slider; 11. Connecting ring; 12. Tension spring; 13. Bidirectional lead screw; 14. Second drive motor; 15. Clamping jaw; 16. Sealing rib; 17. Sealing groove; 18. Linear guide rail; 19. Sliding seat; 20. Reciprocating mechanism. 21. Drive cylinder; 22. Mounting rod; 23. Flexible connecting rod; 24. Paddle body; 25. Support pad; 26. First servo motor; 27. Angle sensor; 28. Bracket; 29. ​​Liquid storage tank; 30. Metering pump; 31. Filling tube; 32. Sliding block; 33. Rotating shaft; 34. Pressing block; 35. Mounting groove; 36. Movable block; 37. First rack; 38. Spring; 39. Transmission gear; 30. Second rack. Detailed Implementation

[0033] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0034] like Figure 1-9 As shown, the pretreatment equipment for E. coli detection in this embodiment includes a base 1, on which a filling station and a rotary drive assembly are provided. A sleeve 2 is fixedly connected to the output end of the rotary drive assembly. The sleeve 2 is fixedly connected to the vertical part of an L-shaped workbench 3 and drives the L-shaped workbench 3 to rotate between the initial station and the processing station. A bag mouth clamping mechanism is connected to one side of the sleeve 2 for fixing the bag mouth of the homogenizing bag at the initial station. A bag body processing mechanism is provided on the horizontal part of the L-shaped workbench 3. The bag body processing mechanism includes a sealing clamp 4 for pressing the bag body and an inertial beating assembly 5 for beating the bag body. A control unit is also provided. The control unit is electrically connected to the rotary drive assembly, the filling station and the bag body processing mechanism. When the L-shaped workbench 3 rotates to the processing station, the filling station fills the homogenizing bag with liquid. Then the sealing clamp 4 presses the bag body and the inertial beating assembly 5 beats the bag body.

[0035] The bag opening clamping mechanism includes a crossbeam 6 fixed to one side of the sleeve 2 and clamping arm assemblies symmetrically arranged at both ends of the crossbeam 6; the clamping arm assembly includes a clamping arm 7, a bag opening clamp 8 and an elastic adaptive module; the clamping arm 7 is composed of a horizontal section and an inclined section; the elastic adaptive module is connected between the bag opening clamp 8 and the end of the inclined section of the clamping arm 7, and is used to buffer the pulling force on the bag opening when the bag opening is closed.

[0036] The elastic adaptive module includes a T-shaped groove 9 horizontally opened on the side of the inclined section of the clamp arm 7. A slidable T-shaped slider 10 is adaptedly installed in the T-shaped groove 9. A connecting ring 11 is fixedly connected to the T-shaped slider 10. The connecting ring 11 is connected to the bag opening clip 8 through a tension spring 12.

[0037] The sealing clamp 4 includes a bidirectional lead screw 13 rotatably mounted on an L-shaped worktable 3. The bidirectional lead screw 13 is driven by a second drive motor 14. Clamping jaws 15 are respectively engaged on the two reverse threads of the bidirectional lead screw 13. The relative clamping surfaces of the two clamping jaws 15 are respectively provided with sealing ribs 16 made of elastic material and sealing grooves 17 adapted to the sealing ribs 16.

[0038] In some embodiments, to facilitate closing the opening of the homogenizing bag, the clamping jaws 15 are not parallel to each other. A sliding block 31 is provided on the clamping jaw 15, and a rotating shaft 32 is fixedly connected to the clamping jaw 15. A torsion spring is provided outside the rotating shaft 32. The rotating shaft 32 is rotatably mounted on the sliding block 31. When the torsion spring is in a free state, the two clamping jaws 15 are arranged in a pattern where one end is close to each other and the other end is far apart. The sliding block 31 is threadedly engaged with the bidirectional lead screw 13. A pressure block 33 is fixedly connected to the clamping jaw 15. The pressure block 33 can cooperate with the bag opening clamp 8 so that the bag opening clamp 8 is engaged. One end of the homogenizing bag is open, and mounting grooves 34 are provided on the opposite sides of the two clamping claws 15. A movable block 35 is movably mounted in the mounting groove 34. The movable block 35 is connected to the inner wall of the mounting groove 34 by a spring 37. A transmission gear 38 is rotatably mounted in the clamping claw 15. A first rack 36 is fixed to the movable block 35, and a second rack 39 is fixed to the pressure block 33. The first rack 36 and the transmission gear 38, and the second rack 39 and the transmission gear 38 are all meshed with each other, so that when the movable block 35 is pressed, the pressure block 33 can move upward.

[0039] Most homogenizing bags have a sealing strip at the bag opening. When the sealing strip is pressed and sealed, it is pushed slowly from one end to the other, making it easier to press and seal. Based on this characteristic, the above-mentioned technical solution is designed.

[0040] Furthermore, the bag opening clip 8 has a rotating shaft in its middle, and a return torsion spring is sleeved on the rotating shaft to ensure that the lower ends of the bag opening clip 8 can be tightly clamped together. The position of the bag opening clip 8 clamping the homogenizing bag is shown in the figure, thereby opening the homogenizing bag.

[0041] When the bag opening clamp 8 is clamped onto the homogenizing bag, the bag opening clamp 8 can open the homogenizing bag to facilitate the addition of the pre-prepared liquid. And when it is necessary to seal the homogenizing bag, the two clamp claws 15 are brought closer together to press and close the sealing strip on the homogenizing bag.

[0042] like Figure 8 As shown, when the two clamping jaws 15 approach each other, one end of each jaw 15 contacts first, pressing one end of the sealing strip on the homogenizing bag. Subsequently, the two sliding blocks 31 gradually approach each other, and the two clamping jaws 15 are gradually pressed together, preventing misalignment of the sealing strip. The function of the return torsion spring is that when the two clamping jaws 15 move away from each other and return to their initial position, the return torsion spring allows the two clamping jaws 15 to return to their original position. Figure 8 The style shown.

[0043] As the two clamping claws 15 approach each other to press the sealing strip of the homogenizing bag, the two bag opening clamps 8 also gradually approach each other as the bag opening gradually tightens. Then, the pressing block 33 can press the bag opening clamps 8 together and press the upper half of the two bag opening clamps 8, thereby causing the lower half of the bag opening clamps 8 (the part clamped in the homogenizing bag) to open, so as to achieve the purpose of separating the bag opening clamps 8 from the homogenizing bag.

[0044] As the clamping jaws 15 continue to close, the movable block 35 on them can be pressed. At this time, the first rack 36 on the movable block 35 engages with the transmission gear 38, causing the transmission gear 38 to rotate. The transmission gear 38 can drive the second rack 39, thereby causing the pressure block 33 to move upward. The friction between the pressure block 33 and the bag opening clamp 8 allows the pressure block 33 to drive the bag opening clamp 8 upward, thus preventing the bag opening clamp from clamping the homogenizing bag opening again when the clamping jaws 15 reset. Furthermore, when the clamping jaws 15 reset, the opening of the homogenizing bag can move downward, and the homogenizing bag is located between the two flexible connecting rods 22 to prevent the homogenizing bag from falling and to facilitate the user's removal of it.

[0045] When the spring 37 is in the free state, the movable block 35 extends out of the clamping jaw 15.

[0046] The inertial striking assembly 5 includes a linear guide rail 18 fixed on the horizontal part of the L-shaped worktable 3, a sliding seat 19 slidably fitted on the linear guide rail 18, and the sliding seat 19 is driven by a reciprocating drive cylinder 20 fixed on the L-shaped worktable 3 to perform reciprocating motion; a flexible striking plate is installed on the sliding seat 19 through a swing connection structure.

[0047] The flexible racket includes a mounting rod 21 with its bottom fixed to a sliding seat 19, a flexible connecting rod 22 connected to the top of the mounting rod 21, and a racket body 23 made of flexible material and connected to the end of the flexible connecting rod 22.

[0048] A position sensor for detecting the position of the slide block 19 is provided at one end of the linear guide rail 18.

[0049] Support pads 24 for supporting the horizontal part of the L-shaped worktable 3 are provided on the base 1 at the positions corresponding to the initial station and the processing station.

[0050] The rotary drive assembly includes a first servo motor 25 fixed on the base 1, and the output shaft of the first servo motor 25 is coaxially connected to the bottom end of the sleeve 2 via a coupling; an angle sensor 26 electrically connected to the control unit is also provided on the base 1 to detect the rotation angle of the L-shaped worktable 3.

[0051] The filling station includes a bracket 27 fixed on the base 1, a liquid storage tank 28 set on the bracket 27, a metering pump 29 connected to the liquid storage tank 28, and a filling tube 30 connected to the outlet of the metering pump 29; the outlet end of the filling tube 30 corresponds to the position of the homogenized bag opening fixed by the bag opening clamping mechanism on the processing station; the metering pump 29 is electrically connected to the control unit.

[0052] Embodiments of the present invention:

[0053] The base 1 serves as the overall support, on which a first servo motor 25 is mounted. A sleeve 2 is fixedly fitted on the output shaft of the first servo motor 25. The sleeve 2 serves as the core drive connector of the entire motion platform. The vertical part of an L-shaped worktable 3 is fixedly connected to the sleeve 2 and can rotate with the sleeve 2. The first servo motor 25 drives the L-shaped worktable 3 to precisely switch between the "initial position" (for easy loading and unloading by the operator) and the "processing position" (for filling and tapping) by controlling the rotation of the sleeve 2. Angle sensor 26 ensures accurate angle rotation. To ensure that the L-shaped worktable 3 can stably bear the load after it is in place, support pads 24 are provided on the base 1 at the positions corresponding to these two positions to support the horizontal part of the L-shaped worktable 3.

[0054] On one side of the sleeve 2, directly above the L-shaped workbench 3, a crossbeam 6 is fixed. A pair of clamping arm mechanisms are symmetrically arranged at both ends of the crossbeam 6. Each clamping arm 7 is composed of a horizontal section and a downwardly inclined section, and the end face of the inclined section is a horizontal plane. The key innovation of the bag opening fixation lies in its elastic adaptive design. A T-shaped groove 9 is horizontally opened on the side of the horizontal end of the inclined section of each clamping arm 7. A T-shaped slider 10 is fitted in the T-shaped groove 9 and can slide horizontally along the groove. A connecting ring 11 is fixedly connected to the T-shaped slider 10 for directly clamping the bag opening clamp 8 of the homogenized bag. It is connected to the connecting ring 11 through a flexible tension spring 12.

[0055] After the homogenizing bag is fixed by the bag opening clamp 8, as the L-shaped worktable 3 rotates to the processing station, and the subsequent sealing clamp 4 presses the bag opening from the middle of the bag body (the sealing clamp 4 presses and seals the sealing strip on the homogenizing bag, thereby causing the bag openings of the homogenizing bag to come closer together and seal), the bag opening will tend to close. At this time, the traditional rigid clamp will directly pull the bag opening, which poses a risk of damage. In this design, the closing force of the bag opening will be converted into a pulling force on the bag opening clamp 8. This pulling force is buffered by the tension spring 12 and transmitted to the T-shaped slider 10, causing the T-shaped slider 10 to produce a small adaptive sliding within the T-shaped groove 9 and move closer together. This process effectively absorbs the displacement of the bag opening, ensuring that the bag opening remains open throughout the entire processing process and is not excessively pulled, simulating the action of a person gently holding the bag opening.

[0056] At the processing station, a filling mechanism is installed, which includes a support 27, a liquid storage tank 28, a metering pump 29, and a filling tube 30. The outlet of the filling tube 30 is directly opposite the mouth of the homogenized bag fixed by the clamping mechanism. The metering pump 29 is controlled by the control unit to precisely inject a predetermined volume of liquid into the bag. The entire bag body processing mechanism is integrated on the horizontal part of the L-shaped workbench 3, including a sealing clamp 4 and an inertial tapping assembly 5. The sealing clamp 4 is located below the bag mouth clamp 8 and above the tapping area. It is driven by a second drive motor 14 to a bidirectional lead screw 13, causing the two clamping jaws 15 to move towards or away from each other. One clamping jaw 15 has a rubber sealing rib 16 on its clamping surface, and the other has a matching rubber sealing groove 17. When they are closed, they can tightly press and seal the area near the bag mouth from the outside of the bag body to prevent liquid from splashing out. The inertial tapping assembly 5 is used to simulate human hand tapping. It includes a linear guide rail 18 fixed on the horizontal platform, a sliding seat 19, and a drive for the sliding seat 19 to perform tapping. The high-speed reciprocating drive cylinder 20 has a flexible clapper mounted on the sliding seat 19. The clapper consists of a mounting rod 21 fixed at the bottom, a flexible connecting rod 22 (such as a spring steel or rubber rod) connected to the top of the mounting rod 21, and a clapper body 23 made of flexible materials such as soft silicone. When the reciprocating drive cylinder 20 pushes the sliding seat 19 to reciprocate, the clapper body 23 will gently strike both sides of the homogenized bag like a pendulum due to inertia. The force is moderate and the amplitude is controllable. To accurately control the clapping stroke, a laser rangefinder is set at one end of the linear guide rail 18 to detect the position of the sliding seat 19 and feed it back to the control unit. The equipment is equipped with a control unit with PLC as the core, which is set on the operating table. The control unit is electrically connected to the first servo motor 25, the second drive motor 14, the reciprocating drive cylinder 20, the laser rangefinder, the metering pump 29, and the start / stop button on the operating table, so as to coordinate and realize a fully automatic process from "feeding, rotation, filling, sealing, clapping, and resetting".

[0057] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0058] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A pretreatment device for Escherichia coli detection, comprising a base, characterized in that, A filling station and a rotary drive assembly are mounted on the base. A sleeve is fixedly connected to the output end of the rotary drive assembly. The sleeve is fixedly connected to the vertical part of the L-shaped worktable and drives the L-shaped worktable to rotate between the initial station and the processing station. A bag mouth clamping mechanism is connected to one side of the sleeve to fix the bag mouth of the homogenizing bag at the initial station. A bag body processing mechanism is mounted on the horizontal part of the L-shaped worktable. The bag body processing mechanism includes a sealing clamp for pressing the bag body and an inertial beating assembly for beating the bag body. A control unit is also provided. The control unit is electrically connected to the rotary drive assembly, the filling station, and the bag body processing mechanism. When the L-shaped worktable rotates to the processing station, the filling station fills the homogenizing bag with liquid. Then, the sealing clamp presses the bag body, and the inertial beating assembly beats the bag body. The bag opening clamping mechanism includes a crossbeam fixed to one side of the sleeve and clamping arm assemblies symmetrically arranged at both ends of the crossbeam; the clamping arm assembly includes a clamping arm, a bag opening clamp, and an elastic adaptive module; the clamping arm consists of a horizontal section and an inclined section; the elastic adaptive module is connected between the bag opening clamp and the end of the inclined section of the clamping arm, and is used to buffer the pulling force on the bag opening when the bag opening is closed. The elastic adaptive module includes a T-shaped groove horizontally opened on the side of the inclined section of the clamping arm, a slidable T-shaped slider is adaptedly installed in the T-shaped groove, a connecting ring is fixedly connected to the T-shaped slider, and the connecting ring is connected to the bag mouth clip through a tension spring. The sealing fixture includes a bidirectional lead screw rotatably mounted on an L-shaped worktable. The bidirectional lead screw is driven by a second drive motor. Clamping jaws are respectively engaged on the two reverse threads of the bidirectional lead screw. The relative clamping surfaces of the two clamping jaws are respectively provided with sealing ribs made of elastic material and sealing grooves adapted to the sealing ribs. A sliding block is provided on the clamping jaw, and a rotating shaft is fixedly connected to the clamping jaw. A torsion spring is provided outside the rotating shaft. The rotating shaft is rotatably mounted on the sliding block. When the torsion spring is in a free state, the two clamping jaws are arranged in a pattern where one end is close to each other and the other end is far apart. The sliding block is threadedly engaged with a double-acting screw. A pressure block is fixedly connected to the clamping jaw. The pressure block can engage with a bag opening clamp so that the bag opening clamp bites at one end of the homogenized bag. There are mounting grooves on the opposite sides of the two clamping jaws. A movable block is movably mounted in the mounting groove. The movable block is connected to the inner wall of the mounting groove by a spring. A transmission gear is rotatably mounted in the clamping jaw. A first rack is fixedly connected to the movable block, and a second rack is fixedly connected to the pressure block. The first rack and the transmission gear, and the second rack and the transmission gear are all meshed with each other so that when the movable block is pressed, the pressure block can move upward.

2. The pretreatment device for Escherichia coli detection according to claim 1, characterized in that: The inertial striking assembly includes a linear guide rail fixed to the horizontal part of an L-shaped worktable, a sliding seat slidably fitted on the linear guide rail, and the sliding seat being driven to reciprocate by a reciprocating drive cylinder fixed to the L-shaped worktable. A flexible striking plate is mounted on the sliding seat via a swing connection structure. The flexible striking plate includes a mounting rod fixed at the bottom to the sliding seat, a flexible connecting rod connected to the top of the mounting rod, and a striking body made of flexible material connected to the end of the flexible connecting rod.

3. A pretreatment device for Escherichia coli detection according to claim 2, characterized in that: A position sensor for detecting the position of the sliding block is installed at one end of the linear guide rail.

4. A pretreatment device for Escherichia coli detection according to claim 1, characterized in that: Support pads are provided on the machine base at the positions corresponding to the initial station and the processing station to support the horizontal part of the L-shaped worktable.

5. A pretreatment device for Escherichia coli detection according to claim 1, characterized in that: The rotary drive assembly includes a first servo motor fixed on the base, the output shaft of which is coaxially connected to the bottom end of the sleeve via a coupling; an angle sensor electrically connected to the control unit is also provided on the base for detecting the rotation angle of the L-shaped worktable.

6. A pretreatment device for Escherichia coli detection according to claim 1, characterized in that: The filling station includes a bracket fixed on the machine base, a liquid storage tank set on the bracket, a metering pump connected to the liquid storage tank, and a filling tube connected to the outlet of the metering pump; the outlet end of the filling tube corresponds to the position of the homogenized bag opening fixed by the bag opening clamping mechanism at the processing station; the metering pump is electrically connected to the control unit.

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