A traditional Chinese medicine composition for treating scald and a preparation method thereof

Abstract

The present application relates to the technical field of traditional Chinese medicine composition, and particularly to a traditional Chinese medicine composition for treating scald and a preparation method thereof, and provides a traditional Chinese medicine composition for treating scald, which comprises: honeysuckle stem, lotus leaf, kadsura coccinea, one mouth clock, rehmannia glutinosa, turmeric, phellodendron, raw sanguis draconis, angelica tail, radix violae, ganoderma lucidum, plum slice, natural white beeswax and camellia oil. The scald treatment medicine prepared from the traditional Chinese medicine composition has the advantages of rapid analgesia, no adhesion after dressing change, promotion of wound healing, short healing period, scar repair function and stable quality.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine composition technology, specifically to a traditional Chinese medicine composition for treating burns and its preparation method. Background Technology

[0002] In the field of burn treatment, pain relief, adhesion prevention, rapid healing, and scar repair are key technical concerns in clinical practice, directly impacting patient experience and healing outcomes. While various burn treatment medications and related protocols exist on the market, numerous technical shortcomings remain in practical application, failing to meet treatment needs.

[0003] Existing burn ointments offer slow-acting analgesia, resulting in prolonged patient suffering. Adhesions during dressing changes pose a risk of secondary wound damage. Some formulations are poorly designed, leading to adhesions between the drug carrier or dressing and the wound during dressing changes. Subsequent removal of these adhesive materials can easily cause secondary mechanical damage to the healing wound, not only exacerbating patient pain during dressing changes but also potentially delaying the healing process. Low wound healing efficiency and insufficient early protection limit the effectiveness of these ointments in promoting wound healing, resulting in a prolonged healing period. Furthermore, existing burn ointments lack effective early intervention and protection; even timely application after a burn is insufficient to prevent blister formation and further wound deterioration, hindering subsequent healing. Poor post-healing skin quality hinders scar tissue repair and the restoration of original skin elasticity, leading to scarring that affects the appearance and functional integrity of the skin.

[0004] Given the numerous shortcomings of existing technologies, developing a burn treatment drug that provides rapid pain relief, prevents adhesion during dressing changes, shortens the healing period, and also has scar repair functions while maintaining stable quality has become a pressing technical problem in this field. This invention addresses these technical pain points by optimizing the formula and designing the dosage form to provide a highly effective and safe burn treatment ointment, thus overcoming the deficiencies of existing technologies. Summary of the Invention

[0005] The purpose of this invention is to provide a traditional Chinese medicine composition for treating burns and its preparation method, thereby solving the above-mentioned problems.

[0006] The technical solution of this invention is:

[0007] A traditional Chinese medicine composition for treating burns, comprising the following parts by weight: 10-40 parts of honeysuckle vine, 10-40 parts of hibiscus leaf, 30-60 parts of angelica sinensis, 10-40 parts of angelica dahurica, 30-60 parts of rehmannia glutinosa, 10-40 parts of turmeric, 30-60 parts of phellodendron chinense, 30-60 parts of sanguisorba officinalis, 30-60 parts of angelica sinensis tail, 10-40 parts of ganoderma lucidum, 5-10 parts of borneol, 60-90 parts of natural white beeswax, and 500-600 parts of camellia oil.

[0008] Preferably, the traditional Chinese medicine composition consists of the following parts by weight: 40 parts of honeysuckle vine, 40 parts of hibiscus leaf, 40 parts of angelica sinensis, 40 parts of angelica dahurica, 30 parts of rehmannia glutinosa, 10 parts of turmeric, 30 parts of phellodendron chinense, 30 parts of sanguisorba officinalis, 30 parts of angelica sinensis tail, 10 parts of gromwell root, 10 parts of lingzhi mushroom, 5 parts of borneol, 60 parts of natural white beeswax, and 600 parts of camellia oil.

[0009] A method for preparing a traditional Chinese medicine composition for treating burns includes the following steps:

[0010] 1) Wash, air-dry, and chop the honeysuckle vine, angelica sinensis, angelica dahurica, rehmannia glutinosa, turmeric, phellodendron chinense, sanguisorba officinalis, angelica sinensis tail, and ganoderma lucidum separately and set aside.

[0011] 2) Wash and air-dry the hibiscus leaves and gromwell root separately, then cut them into small pieces and set aside.

[0012] 3) Soak the honeysuckle vine, angelica sinensis, angelica dahurica, rehmannia glutinosa, turmeric, phellodendron chinense, sanguisorba officinalis, angelica sinensis tail, and ganoderma lucidum, which have been chopped in step 1), in two-thirds of their weight of camellia oil for 7-10 days.

[0013] 4) Soak the hibiscus leaves and alkanet root that have been chopped in step 2) in one-third of their weight of camellia oil for 7-10 days;

[0014] 5) Take the honeysuckle vine, angelica sinensis, angelica dahurica, rehmannia glutinosa, turmeric, phellodendron chinense, sanguisorba officinalis, angelica sinensis tail and ganoderma lucidum that have been soaked in camellia oil in step 3), boil them over high heat, then simmer over low heat for 30-40 minutes to obtain medicinal oil A.

[0015] 6) Take the hibiscus leaves and gromwell root that have been soaked in camellia oil in step 4), bring them to a boil over high heat, then simmer over low heat for 10-15 minutes to obtain medicinal oil B.

[0016] 7) Filter the medicinal oil A obtained in step 5) to remove solid impurities, and then let it stand and cool.

[0017] 8) Filter the medicinal oil B obtained in step 6) to remove solid impurities, and then let it stand and cool.

[0018] 9) Dispense the medicinal oil A obtained in step 7) after removing solid impurities into sterile test tubes and seal them.

[0019] 10) Dispense the medicinal oil B obtained in step 8) after removing solid impurities into sterile test tubes and seal them.

[0020] 11) Mix the medicinal oil obtained in step 9) and the medicinal oil obtained in step 10) in a ratio of 2:1 to obtain medicinal oil C. Place it in a sterile glass beaker, stir well and set aside.

[0021] 12) Grind the borneol into a fine powder and set aside;

[0022] 13) Place the glass beaker from step 11) in a water bath and heat it. When the temperature of the medicinal oil C reaches 100°C, add the natural white beeswax and stir well until the natural white beeswax is completely melted.

[0023] 14) Add the borneol powder obtained in step 12) to the medicinal oil C containing natural white beeswax in step 13), and stir well. The resulting liquid ointment is then packaged and sealed for later use.

[0024] 15) Seal the sterile test tube containing the liquid ointment obtained in step 14) and sterilize it using a high-pressure steam sterilizer. After sterilization, wait for it to cool to room temperature to obtain the finished burn ointment.

[0025] Preferably, in steps 9) and 10), the sealed sterile test tubes are stored in a 4°C refrigerator for 3-7 days to remove any residual heat.

[0026] Preferably, in step 14), the obtained liquid ointment is dispensed into sterile test tubes and sealed for later use.

[0027] Preferably, in step 15), the high-pressure sterilization temperature is between 121℃ and 126℃, the pressure is 0.103-0.142MPa, and the sterilization time is 30 minutes.

[0028] The design concepts of the various components in this invention are as follows:

[0029] Polygonum aviculare has a sour taste and slightly cold properties; it enters the lung and bladder meridians; it can clear heat and detoxify, disperse blood stasis and promote blood circulation, soften hardness and reduce swelling, and remove dampness and astringe; it is mainly used to treat all kinds of carbuncles, boils, erysipelas, pemphigus, herpes zoster, eczema, snake bites, burns, bruises, traumatic injuries with blood stasis and swelling, etc.; when used to treat burns, its effects are mainly reflected in relieving pain and promoting the healing of skin damage; Polygonum aviculare contains a variety of active ingredients, which help promote the regeneration and repair of skin tissue, reduce the risk of infection and scar formation.

[0030] Phellodendron bark, bitter in taste and cold in nature; enters the kidney and bladder meridians; it can clear heat and dry dampness, purge fire and eliminate steaming heat, detoxify and heal sores; it is used for damp-heat diarrhea, jaundice with red urine, leukorrhea and vulvar itching, painful urination due to heat, beriberi with weakness and paralysis, bone steaming fever, night sweats, seminal emission, sores and carbuncles, eczema and damp sores; after a burn, local heat toxins may accumulate, and Phellodendron bark can reduce redness, swelling and pain at the burn site and promote wound healing through its heat-clearing and detoxifying effects; at the same time, Phellodendron bark also has certain antibacterial effects and can help prevent infection at the burn site.

[0031] Raw Sanguisorba officinalis has a bitter, sour, and astringent taste, and is slightly cold in nature. It enters the liver and large intestine meridians. It can cool the blood and stop bleeding, detoxify and astringe sores. It is used for hematochezia, hemorrhoidal bleeding, bloody dysentery, metrorrhagia, burns, carbuncles and boils. The tannins and flavonoids in Sanguisorba officinalis have anti-inflammatory, hemostatic, and wound-healing effects. Its sour and astringent properties can astringe sores, reduce exudation, promote granulation tissue growth, accelerate wound healing, and relieve pain and swelling after burns.

[0032] Rehmannia glutinosa, sweet in taste and cold in nature, enters the heart, liver, and kidney meridians. It can clear heat and cool the blood, nourish yin and generate fluids. It is used for heat entering the blood, febrile rashes, hematemesis and epistaxis, yin deficiency due to febrile diseases, red tongue with thirst, constipation due to fluid depletion, yin deficiency fever, bone steaming fever, and internal heat with thirst. In traditional Chinese medicine clinical practice, it is often used as an adjunct treatment for burns. After a burn, the local tissue is damaged, and it is easy to have intense heat and yin fluid depletion. Rehmannia glutinosa can reduce local redness, swelling, heat and pain by clearing heat and cooling the blood, while nourishing yin and generating fluids to promote tissue repair.

[0033] Turmeric has a pungent and bitter taste and is warm in nature; it enters the spleen and liver meridians; it can promote blood circulation and relieve pain; curcumin in turmeric has anti-inflammatory, antioxidant and tissue repair promoting effects; after a burn, a local inflammatory reaction will occur, and curcumin can reduce inflammation, relieve redness, swelling and pain, and promote cell repair, which helps wound healing.

[0034] Angelica tail, the tail part of the Chinese herb Angelica sinensis, has the effect of promoting blood circulation and removing blood stasis. It is often used for injuries from falls and blows, swelling and pain due to blood stasis. When used for burns, it can improve blood supply to the burned area, provide sufficient nutrition for tissue repair, and help wound healing. Its anti-inflammatory properties can reduce the inflammatory response after burns and relieve symptoms such as redness, swelling and pain. For pigmentation or mild scar hyperplasia that may occur after burns, Angelica tail can play a certain auxiliary role in improving the condition by nourishing the skin and promoting the replacement of old and new tissues.

[0035] Natural white beeswax is a white or pale yellow solid, matte and non-crystalline. It possesses detoxifying, astringent, tissue-regenerating, and analgesic properties. Externally, it is used for non-healing ulcers, leg ulcers, external wounds, burns, and scalds. Its main components, such as esters and fatty acids, form a protective film, isolating the wound from air and reducing the risk of infection, while simultaneously promoting wound healing and relieving pain. Compared to ordinary yellow beeswax, natural white beeswax is softer, has better extensibility and plasticity, lower viscosity, and higher purity. Natural white beeswax has natural moisture-proof and water-resistant properties, allowing the ointment to remain stable in humid environments and preventing the medication from deteriorating due to moisture. In addition to its medicinal value, natural white beeswax can adjust the viscosity of the ointment, making it suitable for local application. It also reduces skin irritation, making the ointment gentler and suitable for sensitive skin. Furthermore, some components in natural white beeswax have antibacterial and antioxidant properties, which help improve the antibacterial ability of the ointment, extend its shelf life, and reduce oxidative deterioration during storage.

[0036] Honeysuckle vine, sweet in taste and cold in nature; enters the lung and stomach meridians; can clear heat and detoxify, dispel wind and unblock the meridians; often used for febrile diseases, dysentery due to heat toxicity, carbuncles and sores, rheumatic fever, and red, swollen, hot and painful joints; after burns, local heat toxicity will appear, honeysuckle vine can help clear heat toxicity, reduce local redness, swelling, heat and pain and promote wound healing; when used in synergy with other drugs, it can enhance the effects of clearing heat and detoxifying, reducing swelling and relieving pain, and astringing and promoting tissue regeneration.

[0037] Hibiscus leaves are pungent and cool in nature; they enter the lung and liver meridians; they can cool the blood and detoxify, reduce swelling and relieve pain; they are often used to treat carbuncles, boils, shingles, red and swollen eyes, and traumatic injuries; they clear heat and disperse toxins, are effective in treating all kinds of sores, and are good for both topical application and oral administration; hibiscus leaves are cool in nature and can clear heat and cool the blood, reduce local heat toxicity after burns, and relieve redness, swelling, heat and pain; they can promote local blood circulation, reduce inflammatory response, relieve pain, and prevent blister formation and infection; their active ingredients help accelerate granulation tissue growth, promote wound healing, and reduce scar formation.

[0038] Lithospermum erythrorhizon, sweet and salty in taste, and cold in nature; it enters the heart and liver meridians; it can clear heat and cool blood, invigorate blood and detoxify, and promote rash eruption and eliminate spots; it is used for blood heat and toxicity, purplish-black rashes, incomplete measles eruption, sores, eczema, and burns; the shikonin and other components in lithospermum erythrorhizon can inhibit the release of inflammatory factors, reduce redness, swelling, pain and other inflammatory reactions at the burn site, and help relieve discomfort; at the same time, it can inhibit a variety of pathogenic bacteria, such as Staphylococcus aureus, reduce the risk of burn wound infection, and promote wound healing; in addition, shikonin can accelerate wound healing, promote granulation tissue growth, and help repair damaged skin tissue.

[0039] One-Bell Bodhi, also known as Golden Bell Bodhi, is a traditional Chinese folk herbal medicine with the effects of relieving wind and heat, inhibiting bacteria and reducing inflammation, and preventing decay and relieving itching. It can be used externally to treat burns, scalds, cellulitis, etc. Its natural ingredients may help reduce the inflammatory response after burns, promote wound healing, and prevent infection to some extent.

[0040] Reishi mushroom, sweet in taste and neutral in nature, can enter the five internal organs. Externally, it has anti-inflammatory, antipruritic, wound-healing, skin-texturing, and rash-relieving effects. The triterpenoids in reishi mushroom have significant anti-inflammatory activity, inhibiting inflammatory responses and immune responses, and can be used to reduce redness, swelling, heat, and pain. Simultaneously, it contains various alkaloids and trace elements, which help stimulate the growth of new granulation tissue and accelerate the wound repair process. Furthermore, reishi mushroom has a certain inhibitory effect on various pathogenic fungi such as Staphylococcus aureus and Candida albicans, thus playing a certain anti-infective role.

[0041] Borneol, extracted from the trunk of the borneol tree (also known as the borneol tree), a plant of the Lauraceae family, is a natural crystalline compound. It has the functions of clearing heat and detoxifying, preventing decay and promoting tissue regeneration, and relieving pain. Borneol is often combined with other traditional Chinese medicines to make ointments, which are then applied to burns to relieve pain, promote wound healing, and reduce scar formation.

[0042] Camellia oil possesses moisturizing, anti-inflammatory, and analgesic properties. Rich in unsaturated fatty acids and vitamin E, it deeply moisturizes the skin, locks in moisture, and alleviates dryness and peeling. Its small molecular structure allows for easy absorption by the skin, repairing damaged skin barriers, enhancing skin elasticity, and reducing fine lines and wrinkles. For minor skin inflammations (such as acne, eczema, and dermatitis) and insect bites, the tea polyphenols and squalene in camellia oil have antibacterial and anti-inflammatory effects, relieving redness, swelling, and itching, and promoting wound healing. Camellia oil is rich in monounsaturated fatty acids (oleic acid content approximately 80%-90%), a fatty acid structure that makes it more stable at high temperatures and less prone to oxidation and decomposition into harmful substances. Studies show that camellia oil maintains good stability at oil temperatures of 180℃-220℃, minimizing the production of large amounts of fumes and harmful substances, making it ideal as a base for burn ointments due to its excellent skin affinity.

[0043] Principles of compound formulation:

[0044] Principal herbs: Polygonum aviculare, Phellodendron chinense, and Sanguisorba officinalis;

[0045] The main ingredients are: Rehmannia glutinosa, Curcuma longa, Angelica sinensis tail, and natural white beeswax.

[0046] Medicinal herbs: honeysuckle vine, hibiscus leaf, gromwell root, and a small bell;

[0047] Used medicinal ingredients: borneol, Ganoderma lucidum.

[0048] Solution:

[0049] The principal herbs are Phellodendron bark and Sanguisorba officinalis, which complement each other. Phellodendron bark is bitter and cold, and is good at clearing heat and drying dampness, purging fire and detoxifying, especially for damp-heat. Sanguisorba officinalis is bitter, cold and astringent, and can clear heat, cool blood, detoxify and astringe sores. The combination of the two can enhance the heat-clearing and detoxifying power, and is used for heat-toxin sores, burns and scalds. The astringent nature of Sanguisorba officinalis gives it the effects of astringing and stopping bleeding, and astringing dampness and healing sores. Phellodendron bark clears heat and dries dampness, and can promote the healing of sores. The combination of the two can promote the repair of damaged skin and mucous membranes, reduce exudation and accelerate healing. Polygonum multiflorum has the effects of clearing heat and detoxifying, and promoting diuresis and reducing swelling. When used to treat burns, it can relieve pain, reduce wound edema, help promote the regeneration and repair of skin tissue, and reduce the risk of infection and scar formation. These three Chinese medicines, as the principal herbs, work together to clear heat and cool blood, detoxify and astringe sores, and reduce swelling and relieve pain.

[0050] The formula combines four herbs: Rehmannia glutinosa, Curcuma longa, Angelica sinensis tail, and natural white beeswax. Rehmannia glutinosa is sweet and cold in nature, and can clear heat, cool the blood, nourish yin, and generate fluids. Curcuma longa is bitter and warm in nature, and can promote blood circulation and relieve pain. After a burn, the local tissue is damaged, and there is a tendency for excessive heat and toxicity and depletion of yin fluids. The combination of these two herbs, with their sweet and bitter properties and their warm and cold properties, can clear heat, cool the blood, nourish yin, generate fluids, promote qi circulation, and relieve pain, thereby reducing local redness, swelling, heat, and pain. After a burn, there is often local bruising. Angelica sinensis tail has the effect of promoting blood circulation and removing blood stasis, nourishing blood and promoting blood circulation without leaving stasis, which can reduce pigmentation and scar hyperplasia after a burn and promote the replacement of old and new tissues. Natural white beeswax can astringe sores, promote tissue regeneration, and relieve pain. Combined with the other herbs, it can promote wound healing and relieve pain. At the same time, natural white beeswax also has antibacterial and preservative properties, which can extend the shelf life of the medicine.

[0051] The adjuvant herbs consist of four ingredients: honeysuckle vine, hibiscus leaf, gromwell root, and a small bell. Honeysuckle vine, hibiscus leaf, and gromwell root can all clear heat, cool the blood, and detoxify. Together, these three herbs help the principal and assistant herbs clear heat and toxins, reducing local redness, swelling, heat, and pain. The small bell can dispel wind and heat, inhibit bacteria, and relieve itching. Combined with gromwell root, it can improve skin itching during the burn healing process, help reduce inflammation, accelerate wound healing, promote granulation tissue growth, and repair damaged skin tissue. The adjuvant herbs can assist the principal and assistant herbs in enhancing the therapeutic effect, while also improving symptoms such as skin itching.

[0052] The main ingredients are borneol and Ganoderma lucidum, which are the finishing touches. Borneol is pungent and has a strong penetrating ability, which can help other drugs penetrate the skin and mucous membranes, "paving the way" for the drug's efficacy and guiding the main drug directly to the disease site. Ganoderma lucidum enters the five internal organs, balances yin and yang, and can harmonize the various drugs, making the overall medicine more mild.

[0053] Beneficial effects:

[0054] 1. The burn ointment prepared from the traditional Chinese medicine composition of this invention has a rapid analgesic effect, and can quickly relieve pain within 10-15 minutes after being applied to the wound.

[0055] 2. No adhesion during wound dressing changes: Because this preparation is an ointment, the material will not adhere to the wound surface during dressing changes, and will not cause further damage due to dressing changes.

[0056] 3. Short wound healing time: The burn ointment prepared from the herbal composition of this invention has a significant healing-promoting effect, effectively accelerating the wound repair process and significantly shortening the wound healing cycle. Applying it immediately after a burn can effectively prevent blistering.

[0057] 4. Can repair scars: It can promote the formation of type I and type III collagen fibers, promote wound healing, and restore skin elasticity. Attached Figure Description

[0058] Figure 1Comparison of the appearance of burn wounds in mice in the burn model group and the positive drug control group on days 3, 7, and 11 after drug administration;

[0059] Figure 2 Comparison of the appearance of the wounds in mice in the low-dose, medium-dose, and high-dose groups of the burn medication treatment group at 3, 7, and 11 days after medication.

[0060] Figure 3 HE staining and Masson staining sections of the scald model group at 3, 7 and 11 days after medication;

[0061] Figure 4 HE-stained and Masson-stained sections of the positive drug control group at 3, 7 and 11 days of drug administration;

[0062] Figure 5 HE-stained and Masson-stained sections of the low-dose group treated with burn medication on days 3, 7, and 11 of medication.

[0063] Figure 6 HE-stained and Masson-stained sections of the burn treatment group (medium dose) at 3, 7 and 11 days after medication.

[0064] Figure 7 HE-stained and Masson-stained sections of the high-dose group treated with burn medication on days 3, 7, and 11 of medication.

[0065] Figure 8 Sirius red staining results of the burn model group and the positive drug control group on days 3, 7 and 11 after drug administration;

[0066] Figure 9 Sirius red staining results of the low-dose, medium-dose, and high-dose groups of burn medication at 3, 7, and 11 days of medication use.

[0067] Figure 10 Photos showing the appearance of a typical burn case before and after medication; Detailed Implementation

[0068] To make the technical problems, technical solutions, and beneficial effects of the present invention clearer, the present invention will be further described in detail with reference to the embodiments and accompanying drawings. It should be understood that the specific embodiments described herein are merely for explaining the present invention and are not intended to limit the present invention. The technical solutions of the present invention will be described in detail below with reference to the embodiments, but the scope of protection is not limited thereto.

[0069] Example 1:

[0070] The proportions of each component in the low-dose group are as follows: 10 parts honeysuckle vine, 10 parts hibiscus leaf, 30 parts angelica sinensis, 10 parts bupleurum, 30 parts rehmannia root, 10 parts turmeric, 30 parts phellodendron bark, 30 parts sanguisorba officinalis, 30 parts angelica sinensis tail, 10 parts gromwell root, 10 parts borneol, 5 parts natural white beeswax, and 500 parts camellia oil.

[0071] According to the above dosage, wash, air-dry, and chop the honeysuckle vine, angelica sinensis, angelica dahurica, rehmannia glutinosa, turmeric, phellodendron chinense, sanguisorba officinalis, angelica sinensis tail, and ganoderma lucidum separately and set aside.

[0072] Wash and air-dry the hibiscus leaves and gromwell root separately, then cut them into small pieces and set aside.

[0073] Soak the above-mentioned shredded honeysuckle vine, angelica sinensis, angelica dahurica, rehmannia glutinosa, turmeric, phellodendron chinense, sanguisorba officinalis, angelica sinensis tail, and ganoderma lucidum in two-thirds of their weight of camellia oil for 7-10 days.

[0074] Soak the above-mentioned shredded hibiscus leaves and alkanet root in one-third of their weight of camellia oil for 7-10 days.

[0075] The above-mentioned honeysuckle vine, angelica sinensis, angelica dahurica, rehmannia glutinosa, turmeric, phellodendron chinense, sanguisorba officinalis, angelica sinensis tail and lingzhi mushroom, which have been soaked in camellia oil, are boiled over high heat until boiling, then simmered over low heat for 30-40 minutes to obtain medicinal oil A.

[0076] The above-mentioned hibiscus leaves and gromwell root soaked in camellia oil are boiled over high heat, then simmered over low heat for 10-15 minutes to obtain medicinal oil B. The two medicinal oils A and B obtained above are filtered to remove solid impurities and then allowed to stand and cool.

[0077] The two medicinal oils A and B obtained above after removing solid impurities were separately dispensed into sterile test tubes and sealed. The sealed sterile test tubes were stored in a 4°C refrigerator for 3-7 days to remove the fire toxicity.

[0078] The two medicinal oils A and B obtained above are mixed in a ratio of 2:1 to obtain medicinal oil C, which is placed in a sterile glass beaker, stirred evenly and set aside for later use.

[0079] Grind the borneol into a fine powder and set aside.

[0080] Place the glass beaker in a water bath and heat it. When the temperature of the medicinal oil C reaches 100°C, add the natural white beeswax and stir well until the natural white beeswax is completely melted.

[0081] Add the obtained borneol powder to the medicinal oil C containing natural white beeswax and stir well. Dispense the resulting liquid ointment into sterile test tubes and seal for later use.

[0082] Seal the sterile test tube containing the liquid ointment obtained above, and sterilize it using a high-pressure steam sterilizer. The high-pressure sterilization temperature is between 121℃ and 126℃, the pressure is 0.103-0.142MPa, and the sterilization time is 30 minutes. After sterilization, let it cool to room temperature to obtain the finished burn ointment.

[0083] Example 2:

[0084] The proportions of each component in the medium-dose group are as follows: 25 parts honeysuckle vine, 25 parts hibiscus leaf, 45 parts angelica sinensis, 25 parts bupleurum, 45 parts rehmannia root, 25 parts turmeric, 45 parts phellodendron bark, 45 parts sanguisorba officinalis, 45 parts angelica sinensis tail, 25 parts gromwell root, 25 parts borneol, 7.5 parts natural white beeswax, and 550 parts camellia oil; the burn ointment was prepared according to the method in Example 1.

[0085] Example 3:

[0086] The high-dose group was prepared with the following proportions: 40 parts honeysuckle vine, 40 parts hibiscus leaf, 60 parts angelica sinensis, 40 parts bupleurum, 60 parts rehmannia root, 40 parts turmeric, 60 parts phellodendron bark, 60 parts sanguisorba officinalis, 60 parts angelica sinensis tail, 40 parts gromwell root, 40 parts borneol, 10 parts borneol, 90 parts natural white beeswax, and 600 parts camellia oil. The burn ointment was prepared according to the method in Example 1.

[0087] Animal experiments:

[0088] Experimental animals: female BALB / c mice, 6-8 weeks old, weighing approximately 20g.

[0089] Preparation: General anesthesia with isoflurane, then shave the hair on the back. The shaved area should be larger than 1cm in diameter to facilitate modeling. Wipe the skin with sterile wet wipes.

[0090] Modeling: Prepare 80℃ hot water, immerse a 10g metal weight (1cm in diameter) in the hot water, then remove it and burn the shaved area on the back of the mouse for 6 seconds, with the wound area about 1cm in diameter, resulting in a grade II burn. After that, keep the mice in individual cages.

[0091] Grouping and administration: The burn model group was divided into 5 groups: a burn model group (no treatment after burn), a positive drug control group (common Mebo burn ointment, twice a day, administered immediately after burn), a low-dose burn treatment group (using the ointment prepared in Example 1 twice a day, administered immediately after burn), a medium-dose burn treatment group (using the ointment prepared in Example 2 twice a day, administered immediately after burn), and a high-dose burn treatment group (using the ointment prepared in Example 3 twice a day, administered immediately after burn).

[0092] Measurement indicators: Five sets of photographs were taken to observe the wound healing status (3, 7, 11 days). Local skin tissue was taken from the burn area and sectioned (3, 7, 11 days). HE staining was used to observe the skin tissue morphology, Sirius red staining was used to observe the proportion of different collagen fibers, and Masson staining was used to observe the distribution of collagen fibers. The results comprehensively reflect the burn healing status.

[0093] Samples were taken from five groups on days 3, 7, and 11. Scalded mice were euthanized by cervical dislocation. Animal ethics protection was strictly followed during the procedure. Mice were disinfected in 75% ethanol solution for 60 seconds and then transferred to a clean bench. The scalded wounds on the back of the mice were separated using microsurgical scissors, about 0.3 cm from the scalded wound edge. The free wound edge skin grafts were transferred to a surgical dissection microscope and trimmed to be smooth and regular. They were fixed with paraformaldehyde for 24 hours, dehydrated with ethanol of varying concentrations, and then embedded in paraffin to form tissue blocks.

[0094] HE staining:

[0095] Paraffin sections were baked in an oven at 60°C for 1 hour. Five groups of sections were then placed in xylene I for 20 min, xylene II for 20 min, anhydrous ethanol I for 5 min, anhydrous ethanol II for 5 min, and 75% ethanol for 5 min. After washing with tap water for 10 s, the sections were stained with hematoxylin for 4 min, washed with tap water for 10 s, differentiated with differentiation solution for 5 s, washed with tap water for 10 s, and then blued with a blueing solution. After rinsing with running water for 10 s, the sections were then placed in a series of 85% and 95% ethanol solutions for 5 min each, stained with eosin for 5 min, and then placed in anhydrous ethanol I for 5 min, anhydrous ethanol II for 5 min, anhydrous ethanol III for 5 min, xylene I for 5 min, and xylene II for 5 min for clearing. The sections were then mounted with neutral resin.

[0096] Sirius Red dyeing:

[0097] Five groups of slides were placed in xylene I for 20 min, xylene II for 20 min, anhydrous ethanol I for 5 min, anhydrous ethanol II for 5 min, and 75% ethanol for 5 min. After washing with tap water, the slides were stained with Sirius red for 8 min, dehydrated with anhydrous ethanol, cleared in xylene for 5 min, mounted with neutral resin, examined under a microscope, and the images were acquired and analyzed.

[0098] Masson staining:

[0099] 5 sets of experimental steps:

[0100] Dewaxing paraffin sections to water: Immerse the sections sequentially in xylene I for 20 minutes, xylene II for 20 minutes, anhydrous ethanol I for 10 minutes, anhydrous ethanol II for 10 minutes, 95% ethanol for 5 minutes, 90% ethanol for 5 minutes, 80% ethanol for 5 minutes, and 70% ethanol for 5 minutes, and finally wash with distilled water.

[0101] Staining cell nuclei with iron hematoxylin: Immerse the sections in iron hematoxylin staining solution for 3-8 minutes, then rinse with running water.

[0102] Staining with Ponceau S-Acid Fuchsin: Place the slides in Ponceau S-Acid Fuchsin staining solution for 5 minutes, then rinse with 0.2% glacial acetic acid.

[0103] Phosphomolybdic acid differentiation: The slices were placed in phosphomolybdic acid for differentiation for a few seconds, and then washed with water.

[0104] Aniline blue staining: Immerse the slides in aniline blue staining solution for 5 minutes, then rinse with 0.2% glacial acetic acid.

[0105] Rapid dehydration and clearing: Dehydrate and clear the sections in the following steps: Immerse the sections in 95% alcohol I for 5 minutes, 95% alcohol II for 5 minutes, anhydrous ethanol I for 5 minutes, anhydrous ethanol II for 5 minutes, xylene I for 5 minutes, and xylene II for 5 minutes. Then remove the sections from the xylene and let them air dry slightly. Finally, mount the sections with neutral resin.

[0106] Microscopic examination, image acquisition, and analysis.

[0107] Overall Results:

[0108] On the third day after the scald model was established in mice, all mice were in good general condition and active. A circular wound was visible on the back of each mouse; the wound was grayish-white, containing hard, white, coagulative necrotic tissue with poor mobility. The mice reacted violently to touch, and the local skin elasticity of the scalded wound was significantly reduced. Compared with the scald model group, the wound area of ​​mice in the low-dose, medium-dose, and high-dose scald medication treatment groups and the positive control group was slightly smaller.

[0109] On day 7 after the establishment of the scald model in mice, all mice in all groups were in good general condition and active. All wounds had scabbed over, the scabs were basically intact, rough, hard, and adhered to the scald wounds with limited mobility, and the wound edges showed a circular white boundary. The wounds were yellowish-white and locally dry. The scald wounds showed the most significant shrinkage in the low-dose, medium-dose, and high-dose groups of the scald medication treatment group.

[0110] On day 11 after the scald model was established in mice, all groups of mice had normal diet and activity. In the scald model group, some scabs fell off the wounds, hair was sparse in the hairless areas, and the wound size did not shrink significantly. In the positive drug control group, hair appeared in the hairless areas, and some scabs remained on the wounds. Compared with the scald model group, the wounds showed improvement, and compared with the same group of mice on day 7, the scald wounds were slightly smaller. In the scald drug treatment groups (low-dose, medium-dose, and high-dose), most of the wounds and scabs fell off naturally, leaving a few scabs, exposing the newly formed pink skin tissue after the scald. Hair appeared in the hairless areas, and the skin elasticity was improved, showing significant improvement.

[0111] On day 3 of HE staining, in the burn model group, tissue necrosis was observed in the epidermal layer of the skin, with a large number of inflammatory cells infiltrating the necrotic tissue, and a large number of inflammatory cells were also observed in the dermal layer.

[0112] On day 3 of Masson staining, in the burn model group, collagen fibers were found to be broken and missing, and the broken areas were necrotic tissue that stained red.

[0113] On day 3 of HE staining, in the positive drug control group, a small amount of tissue loss in the epidermis and inflammatory cell infiltration in the dermis were observed. The degree of inflammatory cell infiltration in the dermis of the skin in the positive drug control group was slightly higher than that in the treatment group.

[0114] On day 3 of Masson staining, in the positive drug control group, the collagen fibers in the epidermal layer of the skin were basically continuously distributed, and the skin collagen fibers were basically healed, with the degree of healing being comparable to that in the treatment group.

[0115] On day 3 of HE staining, in the low-dose, medium-dose, and high-dose groups of the burn medication treatment, fewer chronic inflammatory cells were observed in the epidermis. Compared with the burn model group, the degree of inflammatory cell infiltration in the epidermis was significantly reduced, the epidermal tissue was more intact, and it was covered with a scab. Taking the medium-dose group as an example, a significant reduction in inflammatory cell infiltration in the dermis was observed. Compared with the burn model group, the treatment groups on day 3 effectively reduced the degree of inflammation in the epidermis and dermis of the burned skin tissue and reduced the phenomenon of inflammatory cell infiltration.

[0116] On day 3 of Masson staining, continuous distribution of collagen fibers in the epidermal layer of the burn treatment group (low-dose, medium-dose, and high-dose groups) was observed. No obvious breakage or loss of collagen fibers was seen in the burn site, suggesting that the treatment group effectively promoted wound healing and increased the collagen fiber content in the wound, thereby covering the missing areas of the wound.

[0117] On day 7 of HE staining, the burn model group showed skin loss wounds in the burn tissue, inflammatory cell infiltration within the wounds, and a large number of inflammatory cells infiltrating the dermal tissue.

[0118] In the burn model group on day 7 of Masson staining, broken and missing collagen tissue was visible in the necrotic epidermal layer of the skin. The collagen fibers on the left side were necrotic and unstained, while the collagen fibers on the right side were stained blue.

[0119] On day 7 of HE staining, in the positive drug control group, necrosis of the epidermal tissue and a large number of inflammatory cells infiltrated therein were observed, and a large number of inflammatory cells were observed in the dermis. The degree of inflammation was increased compared with the treatment group and decreased compared with the burn model group.

[0120] On day 7 of Masson staining, in the positive drug control group, less collagen fiber distribution was observed in the dermis. Compared with the treatment group, the number of collagen fibers was less and the continuity of distribution was reduced.

[0121] On day 7 of HE staining, in the low-dose, medium-dose, and high-dose groups of the burn treatment drug, there was basically no obvious tissue loss in the epidermis. Taking the medium-dose group as an example, moderate inflammatory cell infiltration was observed in the dermis.

[0122] On day 7 of Masson staining, in the low-dose, medium-dose, and high-dose groups of the burn medication treatment, a greater amount of collagen fibers was observed in the dermis, and the overall distribution of collagen fibers in the skin increased, effectively promoting wound healing.

[0123] On day 11 of HE staining, in the burn model group, small tissue loss was observed in the epidermal layer of the skin, with inflammatory cell infiltration within it; and a large number of inflammatory cells were observed in the dermal layer below the wound.

[0124] Masson staining on day 11 showed disordered collagen fiber tissue components in the dermis of the scald model group, with poor continuity in collagen fiber distribution.

[0125] On day 11 of HE staining, the positive drug control group showed a relatively continuous epidermal layer, and inflammatory cell infiltration was visible in the dermis.

[0126] On day 11 of Masson staining, the positive drug control group showed a more disordered distribution of collagen fibers in the dermis, with less integrity, continuity, and quantity compared to the burn treatment group.

[0127] On day 11 of HE staining, continuous distribution of inflammatory cells was observed in the epidermal layer of the low-dose, medium-dose, and high-dose scald medication treatment groups, with no obvious inflammatory cell infiltration. The healing degree was better than that of the scald model group. Taking the medium-dose group as an example, the degree of inflammatory cell infiltration in the dermis was significantly reduced compared with the scald model group, effectively promoting wound healing and reducing inflammatory cell infiltration in the tissue.

[0128] On day 11 of Masson staining, the low-dose, medium-dose, and high-dose groups of the burn treatment drug showed that the distribution of collagen fibers in the dermis was richer than that in the model group, with a greater number of collagen fibers and increased continuity of collagen fibers, effectively promoting the healing of burn wounds.

[0129] Observation under a polarizing microscope showed the following results:

[0130] On day 3, the content of type I and type III collagen fibers in the low-dose, medium-dose, and high-dose groups of the burn medication treatment group was significantly increased compared with the burn model group, and was comparable to that in the positive drug control group.

[0131] On day 7, the content of type I and type III collagen fibers in the low-dose, medium-dose, and high-dose groups of the burn medication treatment group was significantly increased compared to the burn model group, and also higher than that in the positive drug control group.

[0132] On day 11, the content of type I and type III collagen fibers in the low-dose, medium-dose, and high-dose groups of the burn medication treatment group was significantly increased compared to the burn model group, and also higher than that in the positive drug control group.

[0133] The burn ointment of this invention can promote the formation of type I and type III collagen fibers, promote wound healing, and restore skin elasticity.

[0134] Typical case:

[0135] scald:

[0136] Mr. Wang, male, 60 years old, suffered a scald to his left lower extremity 12 hours prior. He accidentally scalded his left lower extremity by knocking over a thermos flask 12 hours earlier, experiencing immediate and severe pain. Blisters gradually formed on his left lower extremity, with swelling around the wound. No specific treatment was given. He had no prior medical history. Physical examination revealed normal development and moderate nutrition. He appeared distressed but cooperated with the examination. No lymphadenopathy was observed. The scalded area showed skin abrasion, a bright red base, pinpoint bleeding, and significant exudate. He also presented with a red tongue with a yellow coating, dry mouth, bitter taste, yellow urine, constipation, and a wiry, tense pulse. He was diagnosed with a second-degree burn to his left lower extremity. He was treated with saline solution irrigation, iodine disinfection, and the herbal ointment prepared according to Example 2 of this invention was applied. The patient reported significant pain relief within 10-15 minutes of application. He was instructed to apply the ointment 1-2 times daily. A scab formed after 4 days, and gradually fell off after 10 days. He returned for a follow-up visit after 2 weeks of treatment, and the skin wound had healed. Figure 10 As shown.

[0137] The above description is a further detailed explanation of the present invention in conjunction with specific preferred embodiments. For those skilled in the art, several simple deductions or substitutions can be made without departing from the present invention, and all such deductions or substitutions should be considered as falling within the scope of patent protection determined by the submitted claims.

Claims

1. A traditional Chinese medicine composition for treating burns, characterized in that, It is composed of the following parts by weight: 10-40 parts honeysuckle vine, 10-40 parts hibiscus leaves, 30-60 parts angelica sinensis, 10-40 parts angelica dahurica, 30-60 parts rehmannia glutinosa, 10-40 parts turmeric, 30-60 parts phellodendron chinense, 30-60 parts sanguisorba officinalis, 30-60 parts angelica sinensis tail, 10-40 parts gromwell root, 10-40 parts borneol, 5-10 parts borneol, 60-90 parts natural white beeswax, and 500-600 parts camellia oil.

2. The traditional Chinese medicine composition for treating burns according to claim 1, characterized in that: It is composed of the following parts by weight: 40 parts honeysuckle vine, 40 parts hibiscus leaf, 40 parts angelica sinensis, 40 parts angelica dahurica, 30 parts rehmannia glutinosa, 10 parts turmeric, 30 parts phellodendron chinense, 30 parts sanguisorba officinalis, 30 parts angelica sinensis tail, 10 parts gromwell root, 10 parts lingzhi mushroom, 5 parts borneol, 60 parts natural white beeswax, and 600 parts camellia oil.

3. The method for preparing a traditional Chinese medicine composition for treating burns according to claim 1, characterized in that, Includes the following steps: 1) Wash, air-dry, and chop the honeysuckle vine, angelica sinensis, angelica dahurica, rehmannia glutinosa, turmeric, phellodendron chinense, sanguisorba officinalis, angelica sinensis tail, and ganoderma lucidum separately and set aside. 2) Wash and air-dry the hibiscus leaves and gromwell root separately, then cut them into small pieces and set aside. 3) Soak the honeysuckle vine, angelica sinensis, angelica dahurica, rehmannia glutinosa, turmeric, phellodendron chinense, sanguisorba officinalis, angelica sinensis tail, and ganoderma lucidum, which have been chopped in step 1), in two-thirds of their weight of camellia oil for 7-10 days. 4) Soak the hibiscus leaves and alkanet root that have been chopped in step 2) in one-third of their weight of camellia oil for 7-10 days; 5) Take the honeysuckle vine, angelica sinensis, angelica dahurica, rehmannia glutinosa, turmeric, phellodendron chinense, sanguisorba officinalis, angelica sinensis tail and ganoderma lucidum that have been soaked in camellia oil in step 3), boil them over high heat, then simmer over low heat for 30-40 minutes to obtain medicinal oil A. 6) Take the hibiscus leaves and gromwell root that have been soaked in camellia oil in step 4), bring them to a boil over high heat, then simmer over low heat for 10-15 minutes to obtain medicinal oil B. 7) Filter the medicinal oil A obtained in step 5) to remove solid impurities, and then let it stand and cool. 8) Filter the medicinal oil B obtained in step 6) to remove solid impurities, and then let it stand and cool. 9) Dispense the medicinal oil A obtained in step 7) after removing solid impurities into sterile test tubes and seal them. 10) Dispense the medicinal oil B obtained in step 8) after removing solid impurities into sterile test tubes and seal them. 11) Mix the medicinal oil A obtained in step 9) and the medicinal oil B obtained in step 10) in a ratio of 2:1 to obtain medicinal oil C. Place it in a sterile glass beaker, stir well and set aside. 12) Grind the borneol into a fine powder and set aside; 13) Place the glass beaker from step 11) in a water bath and heat it. When the temperature of the medicinal oil C reaches 100°C, add the natural white beeswax and stir well until the natural white beeswax is completely melted. 14) Add the borneol powder obtained in step 12) to the medicinal oil C containing natural white beeswax in step 13), and stir well. The resulting liquid ointment is then packaged and sealed for later use. 15) Seal the sterile test tube containing the liquid ointment obtained in step 14) and sterilize it using a high-pressure steam sterilizer. After sterilization, wait for it to cool to room temperature to obtain the finished burn ointment.

4. The preparation method according to claim 3, characterized in that, In steps 9) and 10), the sealed sterile test tubes are stored in a 4°C refrigerator for 3-7 days to remove any residual heat.

5. The preparation method according to claim 3, characterized in that, In step 14), the obtained liquid ointment is dispensed into sterile test tubes and sealed for later use.

6. The preparation method according to claim 3, characterized in that, In step 15), the high-pressure sterilization temperature is between 121℃ and 126℃, the pressure is 0.103-0.142MPa, and the sterilization time is 30 minutes.

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