Carboxypeptidase B protein monoclonal antibody, kit based on carboxypeptidase B protein monoclonal antibody and application of carboxypeptidase B protein monoclonal antibody
By using a double-antibody sandwich ELISA kit designed with highly specific monoclonal antibodies BE-1E12B8 and BE4-2B6A6, the sensitivity and specificity issues in the detection of recombinant carboxypeptidase B protein were resolved, enabling rapid and accurate quantitative detection.
CN121495873APending Publication Date: 2026-02-10SHANGHAI YAXIN BIOTECHNOLOGY LTD CO
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Patent Information
- Application Number
- CN202610017819.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-08
- Publication Date
- 2026-02-10
AI Technical Summary
Technical Problem
Existing methods for detecting recombinant carboxypeptidase B protein have low sensitivity, poor specificity, and are complex to operate, making it difficult to achieve efficient quantitative detection.
Method used
A double-antibody sandwich ELISA kit was designed using two monoclonal antibodies, BE-1E12B8 and BE4-2B6A6, which have high specificity and sensitivity for secreting carboxypeptidase B. The kit was used to detect different epitopes of carboxypeptidase B protein.
Benefits of technology
This method enables highly sensitive, rapid, and accurate quantitative detection of recombinant carboxypeptidase B protein, simplifies the operation process, and improves the specificity and precision of the detection.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a carboxypeptidase B protein monoclonal antibody, a kit based on the monoclonal antibody and application, and belongs to the technical field of antibody preparation and biological detection. Specifically, two monoclonal antibodies, namely, BE-1E12B8 and BE4-2B6A6, which have binding characteristics with carboxypeptidase B are obtained through screening, the BE-1E12B8 antibody has high titer, and the BE4-2B6A6 antibody has high titer. The BE-1E12B8 and the BE4-2B6A6 are matched for use and are used for sandwich ELISA (enzyme-linked immunosorbent assay), and the carboxypeptidase B can be efficiently detected. Therefore, a double-antibody sandwich ELISA method is established by taking the two monoclonal antibodies as a capture antibody and a detection antibody respectively, the monoclonal antibodies can be bound to different epitopes of carboxypeptidase B respectively, the binding positions are proper, so that the monoclonal antibodies can be jointly used for realizing the detection of the double-antibody sandwich method, and the monoclonal antibodies have better sensitivity and specificity compared with the existing detection method; the method has the advantages of high repeatability and high flux.
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