Preparation method and application of radix angelicae pubescentis extract

Abstract

The invention discloses a preparation method and application of a radix angelicae pubescentis extract, and particularly relates to the technical field of agricultural biology. The preparation method comprises the following steps: crushing radix angelicae pubescentis, sieving with a 40-100-mesh sieve, extracting with absolute ethyl alcohol, and concentrating under reduced pressure to obtain a pasty extract; in the aspect of application, the seed soaking agent is applied to rice, wheat or corn planting, for example, seed soaking treatment is performed on rice, wheat or corn, and the concentration is 10-5000 mg / L, preferably 500 mg / L. The extract is natural, safe and environment-friendly, and conforms to the development strategy of green agriculture. The fertilizer can promote rapid growth of rice, wheat or corn in the seedling stage, help to establish ecological competitive advantages, and relieve weed prevention and control pressure; and the resistance of rice, wheat or corn to adversities such as drought, salt stress and the like can be enhanced. Compared with a traditional growth promoting material and a compound preparation, the preparation process is simple and convenient, the cost is low, more farmers can accept the growth promoting material, breakthrough progress is brought to the rice, wheat or corn planting industry, and the development of a planting area is promoted.

Description

Technical Field

[0001] This invention relates to the field of agricultural biotechnology, and in particular to a method for preparing and applying an extract of Angelica pubescens. Background Technology

[0002] Rice is the staple food for more than 65% of my country's population, but the control of weeds in rice paddies currently faces severe challenges:

[0003] 1. The control of weeds in paddy fields has long relied on chemical herbicides, coupled with the widespread adoption of simplified cultivation methods. This has gradually changed the weed composition of paddy fields and accelerated the rapid development of herbicide resistance in weeds. To ensure weed control effectiveness, many farmers have had to increase the amount of herbicides they use.

[0004] 2. The unscientific, unreasonable, and excessive use of pesticides has further triggered ecological problems such as agricultural non-point source pollution, creating a vicious cycle.

[0005] Farmers often say that "water is the best herbicide," and scientific field water management can indeed alleviate the pressure of weed control in the early stages of rice cultivation, while also offering the advantages of being green and environmentally friendly. However, this method requires a high level of planting experience from farmers: if the water is released too early, although it can suppress weeds, it will have a significant negative impact on the rice seedlings; if the water is released too late, rice and weeds often emerge simultaneously, and as a C3 plant, rice is less likely to gain dominance in the early stages of growth compared to weeds, which are mainly C4 plants, thus greatly reducing the weed control effect.

[0006] Currently, although plant-derived materials are used in rice cultivation, they primarily focus on pest and disease control, with a significant shortcoming in enhancing rice's own stress resistance. During its growth, rice frequently faces abiotic stresses such as drought and salt stress, as well as biotic stresses like pests and diseases. These adverse factors severely impact rice growth, development, and yield stability. Especially against the backdrop of intensifying climate change and the continuous expansion of rice cultivation areas, the need to improve rice's stress resistance is becoming increasingly urgent. Summary of the Invention

[0007] The present invention aims to provide a method for preparing and applying Angelica pubescens extract, which solves the problem that existing plant-derived materials cannot enhance the stress resistance of rice.

[0008] To achieve the above objectives, the technical solution of the present invention is as follows: an application of Angelica pubescens extract, wherein the Angelica pubescens extract is used in the cultivation of rice, wheat or corn.

[0009] Furthermore, the Angelica pubescens extract is used to soak rice, wheat, or corn seeds.

[0010] Furthermore, the concentration of the Angelica pubescens extract is 10–5000 mg / L.

[0011] Furthermore, the concentration of the Angelica pubescens extract is 500 mg / L.

[0012] Compared with existing technologies, the beneficial effects of this solution are:

[0013] 1. In rice, wheat, or corn cultivation, traditional growth promoters such as gibberellin (GA3), ethephon, and ferrous dihydroporphyrin are all chemical agents, and their use as agricultural inputs faces numerous restrictions. These chemicals are highly likely to remain in the soil and rice plants during use, causing not only continuous pollution to the ecological environment but also posing a potential threat to human health. In contrast, Angelica pubescens extract, as a plant-derived material, possesses natural, safe, and environmentally friendly characteristics, effectively reducing negative impacts on the ecological environment. This represents a breakthrough for the rice cultivation industry, promoting the growth of rice, wheat, or corn and enhancing the stress resistance of rice.

[0014] 2. Existing plant materials such as Sichuan pepper and Sichuan lovage rhizome, as well as natural extracts such as humic acid, have revealed problems such as insufficient targeting and poor activity stability in practical applications. This makes it difficult for them to fully exert their effects in complex environmental conditions such as saline-alkali land and arid regions, and they cannot meet the growth needs of rice under various adverse conditions. In contrast, Angelica pubescens extract, with its unique mechanism of action, can adapt to different ecological environments and has shown excellent and stable effects in promoting the growth and development of rice, wheat, or corn and enhancing plant resistance to adverse conditions. It provides a reliable guarantee for the normal growth of rice, wheat, or corn under various adverse conditions and has powerfully promoted the expansion of rice, wheat, or corn planting areas.

[0015] 3. Compared to the single Angelica pubescens extract in this solution, the manufacturing process of common compound preparations of plant hormones and plant extracts is more complex and costly. The cumbersome production process not only increases technical difficulty and production cycle but also significantly drives up product prices, increasing the economic burden on farmers. As a single plant-derived extract, Angelica pubescens extract has a relatively simple manufacturing process, effectively reducing production costs, significantly improving product cost-effectiveness, and making it more accessible and usable by more farmers, thus laying a solid foundation for the widespread adoption of this technology.

[0016] 4. Although some plant-derived materials have been applied in rice cultivation, most focus only on pest and disease control, exhibiting significant shortcomings in promoting crop growth and enhancing the stress resistance of rice, wheat, or corn. Angelica pubescens extract, however, possesses multiple benefits. It not only promotes rapid seedling growth in rice, wheat, or corn, helping them establish an ecological competitive advantage during the seedling stage and effectively reducing weed control pressure, but also significantly enhances their resistance to abiotic stresses such as drought and salt stress, as well as some biotic stresses. Particularly noteworthy is the pioneering application of Angelica pubescens extract in promoting the growth of rice, wheat, or corn, a groundbreaking innovation that fills a research gap in this field. Attached Figure Description

[0017] Figure 1 This is a graph showing the growth-promoting effect of soaking rice seeds with different concentration gradients of Angelica pubescens extract in this embodiment.

[0018] Figure 2 This embodiment describes the effect of soaking seeds with Angelica pubescens extract on the germination of rice seeds treated with 0.6% NaCl.

[0019] Figure 3 This embodiment describes the effect of soaking seeds with Angelica pubescens extract on the germination of rice seeds treated with 0.9% NaCl.

[0020] Figure 4 This embodiment describes the effect of soaking seeds with Angelica pubescens extract on the germination of rice seeds treated with 1.2% NaCl.

[0021] Figure 5 This is a graph showing the effect of soaking seeds with Angelica pubescens extract on the growth of rice seedlings treated with 0.6% NaCl in this embodiment.

[0022] Figure 6 This is a graph showing the effect of soaking seeds with Angelica pubescens extract on the growth of rice seedlings treated with 0.9% NaCl in this embodiment.

[0023] Figure 7 This is a graph showing the effect of soaking seeds with Angelica pubescens extract on the growth of rice seedlings treated with 1.2% NaCl in this embodiment.

[0024] Figure 8 This is a graph showing the effect of soaking seeds with Angelica pubescens extract on the germination of rice seeds treated with 10% PEG6000 in this embodiment.

[0025] Figure 9 This is a graph showing the effect of soaking Angelica pubescens extract on the germination of rice seeds treated with 15% PEG6000 in this embodiment.

[0026] Figure 10 This is a graph showing the effect of soaking seeds with Angelica pubescens extract on the growth of rice seedlings treated with 5% PEG6000 in this embodiment.

[0027] Figure 11 This is a graph showing the effect of soaking Angelica pubescens extract on the growth of rice seedlings treated with 10% PEG6000 in this embodiment.

[0028] Figure 12 This is a graph showing the effect of Angelica pubescens extract soaking on the growth of rice seedlings treated with 15% PEG6000 in this embodiment.

[0029] Figure 13This is a graph showing the effect of soaking seeds with Angelica pubescens extract on the growth of wheat seedlings treated with 0.9% NaCl in this embodiment;

[0030] Figure 14 This is a graph showing the effect of soaking seeds with Angelica pubescens extract on the growth of wheat seedlings treated with 15% PEG6000 in this embodiment.

[0031] Figure 15 This is a graph showing the effect of soaking seeds with Angelica pubescens extract on the growth of maize seedlings treated with 0.9% NaCl in this embodiment.

[0032] Figure 16 This is a graph showing the effect of soaking Angelica pubescens extract on the growth of maize seedlings treated with 15% PEG6000 in this embodiment. Detailed Implementation

[0033] The present invention will be further described in detail below through specific embodiments:

[0034] Example

[0035] An application of Angelica pubescens extract is described, which is used in the cultivation of rice, wheat, and corn. When using the extract in this embodiment, the solubility of the extract must first be addressed. 5000 mg of Angelica pubescens extract is weighed, and the paste-like extract is dissolved in 1 mL of DMSO reagent. The solution is stirred with a glass rod, and then 1 mL of Tween 20 is added. A seed soaking solution is prepared using 1000 mL of water to obtain a 5000 mg / L Angelica pubescens extract stock solution.

[0036] Experimental Method 1: Indoor potted plant method.

[0037] Select plump rice seeds and place them in a gauze bag or beaker. Rinse them 2-3 times with running water to remove surface impurities before soaking. Dilute the above-mentioned Angelica pubescens extract stock solution to 500mg / L, 250mg / L, 100mg / L, 50mg / L, 25mg / L, and 10mg / L according to the specified ratio. Soak the seeds in each diluted solution for 24 hours. After soaking, disinfect the rice seeds in a 1% sodium hypochlorite solution for 15 minutes. After soaking, rinse thoroughly with plenty of water. After cleaning, place the rice seeds in a plastic basin or on gauze, keeping them appropriately moist, and germinate them in a greenhouse at 25-28℃. When 70%-80% of the seeds show signs of germination, they can be sown.

[0038] In this experiment, the rice seeds used were Jingliangyou 534 rice seeds. Seeds soaked in water (as the control group) and seeds soaked in different dilutions were sown in 13×8×5cm plastic square boxes (e.g., Figure 1 As shown, Figure 1From left to right, the groups are CK group, 10 mg / L group, 25 mg / L group, 50 mg / L group, 100 mg / L group, 250 mg / L group, and 500 mg / L group, with the same number of seeds in each group. Each plastic container also contains 200g of air-dried, herbicide-free soil that has been sieved through a 3mm sieve and mixed with 100mL of tap water to form a slurry. Twenty seeds are sown per container, with four replicates per treatment. The containers are placed in a controlled-light cultivation room, and the treated plants are transferred to the cultivation room after sowing. Seven days after sowing, the growth of the rice is observed visually, and the effects of Angelica pubescens extract on the germination potential, germination rate, and growth of the rice are investigated.

[0039] Based on the measurement results, the effects of different concentrations of Angelica pubescens extract on rice growth promotion were calculated. All data are expressed as mean ± standard deviation. Statistical analysis was performed using Excel and DPS data analysis software, and one-way ANOVA was used to assess the significance of differences between variables.

[0040] The effects of soaking seeds with different concentration gradients of Angelica pubescens extract on the growth promotion of rice Jingliangyou 534 are shown in Table 1 below.

[0041] Table 1. Growth-promoting effects of different concentration gradients of Angelica pubescens extract on rice Jingliangyou 534.

[0042] Processing group Plant height (cm) CK group 13.75±0.38c 10mg / L group 16.94±0.37b 25mg / L group 18.06±0.67b 50mg / L group 16.94±0.71ab 100mg / L group 17.25±0.49ab 250mg / L group 17.88±0.54ab 500mg / L group 18.81±0.42a

[0043] Note: The data in the table are mean ± standard error. Different lowercase letters after the data in the same column indicate significant differences (P<0.05), and the same applies below.

[0044] pass Figure 1 ( Figure 1 The results of indoor growth measurements (from left to right: 10 mg / L group, 25 mg / L group, 50 mg / L group, 100 mg / L group, 250 mg / L group, 500 mg / L group) and Table 1 show that when the concentration of Angelica pubescens extract is 10–500 mg / L, the rice plant height of the seed soaking treatment is increased by 23.20–36.80% compared with the water soaking treatment (CK group), and the seed soaking of 500 mg / L Angelica pubescens extract increases the height by 36.80%, which shows a significant growth-promoting effect.

[0045] The effects of soaking seeds with Angelica pubescens extract on the germination of rice seeds treated with different NaCl concentrations are shown in Table 2 below.

[0046] Table 2. Effects of Angelica pubescens extract soaking on rice seed germination after different NaCl concentrations.

[0047]

[0048] Combination Figure 2-4(Each figure, from left to right, represents the CK group, the Angelica pubescens soaking group, the NaCl soaking group, and the NaCl + Angelica pubescens soaking group.) As shown in Table 2, for rice seeds not soaked in NaCl, the germination rate increased by 2.22% compared to the water soaking treatment (CK) after soaking in 500 mg / L Angelica pubescens extract. Furthermore, NaCl treatment after soaking the rice seeds in Angelica pubescens extract improved the salt tolerance of the rice. With NaCl treatment alone, the germination rate, shoot length, and root length gradually decreased with increasing NaCl stress concentration. Soaking the NaCl-soaked rice seeds with 500 mg / L Angelica pubescens extract alone significantly improved the germination rate, shoot length, and root length. Among the treatments, soaking seeds in 500 mg / L Angelica pubescens extract plus 0.9% NaCl increased the germination rate by 40% compared to treatment with 0.9% NaCl alone, while increasing shoot length and root length by 92.81% and 10.64%, respectively. Soaking seeds in 500 mg / L Angelica pubescens extract plus 1.2% NaCl increased the germination rate by 55.55% compared to treatment with 1.2% NaCl alone, while increasing shoot length and root length by 202.00% and 138.89%, respectively. These results indicate that soaking seeds in 500 mg / L Angelica pubescens extract promotes rice germination and effectively improves rice's resistance to NaCl stress.

[0049] The effects of soaking seeds with Angelica pubescens extract on the growth of rice seedlings treated with different NaCl concentrations are shown in Table 3 below.

[0050] Table 3. Effects of Angelica pubescens extract soaking on rice seedling growth after different NaCl concentration treatments.

[0051]

[0052]

[0053] pass Figure 5-7(Each figure, from left to right, represents the CK group, the Angelica pubescens soaking group, the NaCl soaking group, and the NaCl + Angelica pubescens soaking group.) As shown in Table 3, for rice seeds without NaCl soaking, the 500 mg / L Angelica pubescens extract soaking treatment increased plant height, root length, and total chlorophyll content by 23.94%, 65.29%, and 42.69%, respectively, compared to the water soaking treatment (CK). Specifically, the 500 mg / L Angelica pubescens extract soaking + 0.6% NaCl treatment increased plant height, root length, and total chlorophyll content by 13.30%, 14.19%, and 23.88%, respectively, compared to the 0.6% NaCl soaking treatment alone. Soaking seeds in 500 mg / L Angelica pubescens extract plus 0.9% NaCl increased plant height, root length, and total chlorophyll content by 15.23%, 0.29%, and 35.52%, respectively, compared to treatment with 0.9% NaCl alone. Soaking seeds in 500 mg / L Angelica pubescens extract plus 1.2% NaCl increased plant height, root length, and total chlorophyll content by 11.63%, 1.06%, and 90.09%, respectively, compared to treatment with 1.2% NaCl alone. These results indicate that soaking seeds in 500 mg / L Angelica pubescens extract can increase the resistance of rice to NaCl stress, thereby increasing plant height, root length, and total chlorophyll content.

[0054] The effects of soaking seeds with Angelica pubescens extract on the germination of rice seeds treated with different PEG6000 concentrations are shown in Table 4 below.

[0055] Table 4. Effects of Angelica pubescens extract soaking on rice seed germination after treatment with different PEG6000 concentrations.

[0056]

[0057]

[0058] pass Figure 8-9(Each figure, from left to right, represents the CK group, the Angelica pubescens soaking group, the PEG6000 soaking group, and the PEG6000 + Angelica pubescens soaking group.) As shown in Table 4, for rice seeds without PEG6000 soaking, the germination rate of the 500 mg / L Angelica pubescens extract soaking treatment was 4.45% higher than that of the water soaking treatment (CK). Furthermore, the 500 mg / L Angelica pubescens extract soaking treatment also improved the drought resistance of rice. When only PEG6000 was used for stress treatment, the germination rate, shoot length, and root length of the seeds gradually decreased with increasing concentration. When only Angelica pubescens extract was used for soaking, the germination rate, shoot length, and root length of the seeds were significantly improved. Among the treatments, soaking seeds in 500 mg / L Angelica pubescens extract + 5% PEG6000 increased the germination rate by 2.22% compared to treatment with 5% PEG6000 alone, while increasing shoot length and root length by 2.24% and 2.17%, respectively; soaking seeds in 500 mg / L Angelica pubescens extract + 10% PEG6000 increased the germination rate by 6.23% compared to treatment with 10% PEG6000 alone, while increasing shoot length and root length by 0.23% and 24.51%, respectively; soaking seeds in 500 mg / L Angelica pubescens extract + 15% PEG6000 increased the germination rate by 2.22% compared to treatment with 15% PEG6000 alone, while increasing shoot length and root length by 10.30% and 4.13%, respectively; soaking seeds in 500 mg / L Angelica pubescens extract + 20% PEG6000... Compared with the treatment with 20% PEG6000 alone, the germination rate of rice treated with PEG6000 increased by 4.44%, while the shoot length and root length increased by 417.65% and 177.78%, respectively. These results indicate that soaking rice seeds in 500 mg / L Angelica pubescens extract promotes rice germination and effectively improves rice's resistance to PEG6000 stress. This demonstrates that soaking rice seeds in Angelica pubescens extract can increase rice's resistance to PEG6000 stress at certain concentrations.

[0059] The effects of soaking seeds with Angelica pubescens extract on the growth of rice seedlings treated with different PEG6000 concentrations are shown in Table 5 below.

[0060] Table 5. Effects of Angelica pubescens extract soaking on the growth of rice seedlings treated with different PEG6000 concentrations.

[0061]

[0062]

[0063] pass Figure 10-12(Each figure, from left to right, represents the CK group, the Angelica pubescens soaking group, the PEG6000 soaking group, and the PEG6000+Angelica pubescens soaking group.) As shown in Table 5, for rice seeds without PEG6000 soaking, the 500 mg / L Angelica pubescens extract soaking treatment increased plant height, root length, and total chlorophyll content by 12.17%, 2.62%, and 26.91%, respectively, compared to the water soaking treatment (CK). Among them, the treatment of soaking seeds in 500 mg / L Angelica pubescens extract + 5% PEG6000 increased plant height, root length, and total chlorophyll content by 24.62%, 2.07%, and 20.70%, respectively, compared with the treatment of 5% PEG6000 alone; the treatment of soaking seeds in 500 mg / L Angelica pubescens extract + 10% PEG6000 increased plant height, root length, and total chlorophyll content by 29.22%, 13.85%, and 75.52%, respectively, compared with the treatment of 10% PEG6000 alone; and the treatment of soaking seeds in 500 mg / L Angelica pubescens extract + 15% PEG6000 increased plant height, root length, and total chlorophyll content by 7.95%, 19.25%, and 82.64%, respectively, compared with the treatment of 15% PEG6000 alone. The above results indicate that soaking rice seeds in 500 mg / L Angelica pubescens extract can increase the rice’s resistance to PEG6000 stress, thereby increasing plant height, root length and total chlorophyll content.

[0064] Test Method 2:

[0065] The difference between this experimental method and experimental method 1 is that this experimental method uses wheat seed Jimai 22.

[0066] The effects of soaking seeds with Angelica pubescens extract on the growth of wheat seedlings treated with 0.9% NaCl are shown in Table 6 below.

[0067] Table 6. Effects of Angelica pubescens extract soaking on wheat seedling growth after treatment with 0.9% NaCl.

[0068]

[0069]

[0070] pass Figure 13(From left to right in the figure are the CK group, Angelica pubescens seed soaking group, NaCl seed soaking group, and NaCl + Angelica pubescens seed soaking group) and Table 6 show that for wheat seeds without NaCl soaking, the 500 mg / L Angelica pubescens extract soaking treatment increased plant height and root length by 15.32% and 12.09% respectively compared to the water soaking treatment (CK). Specifically, the 500 mg / L Angelica pubescens extract soaking + 0.9% NaCl treatment increased plant height and root length by 6.38% and 4.37% respectively compared to the 0.9% NaCl treatment alone. These results indicate that 500 mg / L Angelica pubescens extract soaking can increase wheat's resistance to NaCl stress, thereby increasing plant height and root length.

[0071] The effects of soaking seeds with Angelica pubescens extract on the growth of wheat seedlings treated with 15% PEG6000 are shown in Table 7 below.

[0072] Table 7. Effects of Angelica pubescens extract soaking on wheat seedling growth after treatment with 15% PEG6000 concentration.

[0073]

[0074] pass Figure 14 (From left to right in the figure are the CK group, Angelica pubescens seed soaking group, PEG6000 seed soaking group, and PEG6000 + Angelica pubescens seed soaking group) and Table 7 show that for wheat seeds without NaCl soaking, the 500 mg / L Angelica pubescens extract soaking treatment increased plant height and root length by 15.32% and 12.09% respectively compared to the water soaking treatment (CK). Specifically, the 500 mg / L Angelica pubescens extract soaking + 15% PEG6000 treatment increased plant height and root length by 22.77% and 59.75% respectively compared to the 15% PEG6000 treatment alone. These results indicate that 500 mg / L Angelica pubescens extract soaking can increase wheat's resistance to NaCl stress, thereby increasing plant height and root length.

[0075] Test Method 3:

[0076] The difference between this experimental method and experimental method 1 is that this experimental method uses maize seed Zhengdan 958.

[0077] The effects of soaking seeds with Angelica pubescens extract on the growth of maize seedlings treated with 0.9% NaCl are shown in Table 8 below.

[0078] Table 8. Effects of extract soaking on the growth of maize seedlings treated with 0.9% NaCl.

[0079]

[0080] pass Figure 15(From left to right in the figure are the CK group, Angelica pubescens seed soaking group, NaCl seed soaking group, and NaCl + Angelica pubescens seed soaking group) and Table 8 show that for maize seeds without NaCl soaking, the 500 mg / L Angelica pubescens extract soaking treatment increased plant height and root length by 8.38% and 21.72% respectively compared to the water soaking treatment (CK). Specifically, the 500 mg / L Angelica pubescens extract soaking + 0.9% NaCl treatment increased plant height and root length by 36.76% and 46.88% respectively compared to the 0.9% NaCl treatment alone. These results indicate that 500 mg / L Angelica pubescens extract soaking can increase maize's resistance to NaCl stress, thereby increasing plant height and root length.

[0081] Table 9. Effects of Angelica pubescens extract soaking on the growth of maize seedlings treated with 15% PEG6000 concentration.

[0082]

[0083]

[0084] pass Figure 16 (From left to right in the figure are the CK group, Angelica pubescens seed soaking group, PEG6000 seed soaking group, and PEG6000 + Angelica pubescens seed soaking group) and Table 9 show that for maize seeds without NaCl soaking, the 500 mg / L Angelica pubescens extract soaking treatment increased plant height and root length by 8.38% and 21.72% respectively compared to the water soaking treatment (CK). Specifically, the 500 mg / L Angelica pubescens extract soaking + 15% PEG6000 treatment increased plant height and root length by 11.08% and 1.81% respectively compared to the 15% PEG6000 treatment alone. These results indicate that 500 mg / L Angelica pubescens extract soaking can increase maize's resistance to NaCl stress, thereby increasing plant height and root length.

[0085] In conclusion, seed soaking with 500 mg / L Angelica pubescens extract significantly promotes the growth of rice, wheat, and corn, effectively accelerating seedling growth. This allows seedlings to quickly establish dominant ecological niches in the field, extending the window of opportunity for water management, and also helps alleviate weed control pressure in later stages of rice cultivation. It also reduces the use of chemical pesticides, making it a safer and more environmentally friendly plant-based material. Seed soaking with Angelica pubescens extract also enhances crop stress resistance, providing a practical reference for expanding the planting range of rice, wheat, and corn.

[0086] The above are merely embodiments of the present invention, and common knowledge such as specific structures and / or characteristics in the solutions are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. Use of an extract of Angelica acutiloba, characterized in that: The extract of the acanthopanax is used for rice, wheat or corn planting.

2. The use of a Rehmannia glutinosa extract according to claim 1, characterized in that: The extract of the acanthopanax is used for rice, wheat or corn planting.

3. The use of a Rehmannia glutinosa extract according to claim 1, characterized in that: The concentration of the extract of the acanthopanax is 10-5000 mg / L.

4. The use of a Rehmannia glutinosa extract according to claim 3, characterized in that: The concentration of the extract of the acanthopanax is 500 mg / L.

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