An ointment for treating scalds and a method for preparing the same

Abstract

This invention belongs to the field of pharmaceutical preparation technology. This invention provides an ointment for treating burns and its preparation method. The method comprises the following steps: 57-60g of sulfur powder and 18-20g of realgar powder are added to 880-890g of tung oil, heated and stirred, then cooled; 2-2.2g of gamboge powder and 1.8-2g of pearl powder are added, stirred, filtered, sterilized, and cooled; 3.2-3.5g of a compound extract is added, mixed well, and cooled to obtain the final product. The preparation method of the compound extract is as follows: A1. By weight, 8-8.5 parts of rhubarb, 6-6.5 parts of artemisia argyi, and 3.2-3.5 parts of scutellaria baicalensis are mixed and pulverized, heated under reflux in an ethanol aqueous solution, and filtered to obtain extract one; A2. 2.3-2.6 parts of coptis chinensis, 2-2.2 parts of gardenia, and 1.5-1.7 parts of dandelion are mixed and pulverized, heated under reflux in an ethanol aqueous solution, and filtered to obtain extract two; A3. The two extracts are concentrated, mixed, and spray-dried to obtain the final product. This invention effectively improves the antibacterial ability of ointments while enhancing their speed and effectiveness in promoting wound healing.

Description

Technical Field

[0001] This invention belongs to the field of pharmaceutical preparation technology, and in particular relates to an ointment for treating burns and its preparation method. Background Technology

[0002] Burns are tissue damage caused by contact with high-temperature substances (such as hot water, steam, hot oil, flames, etc.) on the skin or mucous membranes. They are a common type of physical injury, and their severity depends on the temperature, duration of contact, and location of the injury. Proper identification and treatment are crucial for treatment effectiveness and reducing complications. Burn ointments are topical medications used to treat burns and scalds caused by various reasons. They have antibacterial, analgesic, anti-inflammatory, and wound-healing properties. Currently available burn ointments mainly include two types: synthetic Western medicine and traditional Chinese medicine.

[0003] Among them, representative products of synthetic Western medicines include silver sulfadiazine cream (Silvadene) and mupirocin ointment (Bactroban); their advantages are clear antibacterial effects and stable dosage forms; their limitations are that sulfonamide drugs may cause allergic reactions or leukopenia, long-term use can easily lead to bacterial resistance, and they do not provide sufficient support for wound moisturizing and repair.

[0004] The advantage of traditional Chinese medicine is that it contains natural ingredients and has no toxic side effects; however, its antibacterial / bacteriostatic ability is relatively weak, and its effectiveness is relatively slow, so the speed and effect of promoting wound healing need to be improved. Summary of the Invention

[0005] To address the aforementioned problems, this invention proposes an ointment for treating burns and its preparation method, which can effectively improve the antibacterial ability of the prepared ointment while also enhancing its speed and effectiveness in promoting wound healing.

[0006] To achieve the above objectives, in a first aspect, the present invention provides a method for preparing an ointment for treating burns, comprising the following steps: adding 57-60g of sulfur powder and 18-20g of realgar powder to 880-890g of tung oil, heating to 270±2℃ and stirring continuously for 30-35min, then cooling to 155-160℃; adding 2-2.2g of gamboge powder and 1.8-2g of pearl powder, continuing to stir for 30-35min, filtering, sterilizing, and cooling to 55-60℃; adding 3.2-3.5g of a compound extract, mixing well, and cooling to room temperature to obtain the ointment for treating burns;

[0007] The preparation method of the composite extract is as follows:

[0008] A1. By weight, mix 8-8.5 parts of rhubarb, 6-6.5 parts of mugwort and 3.2-3.5 parts of scutellaria, crush them, add them to an ethanol aqueous solution with a weight of 7-9 times, heat under reflux, filter, and obtain extract one.

[0009] A2. By weight, mix 2.3-2.6 parts of Coptis chinensis, 2-2.2 parts of Gardenia jasminoides and 1.5-1.7 parts of Taraxacum mongolicum, crush them, add them to an ethanol aqueous solution with a weight of 8-10 times, heat under reflux, filter, and obtain extract two.

[0010] A3. Concentrate the first extract and the second extract to a relative density of 1.1 to 1.15 (measured at 60°C), then mix them and spray dry to obtain the composite extract.

[0011] Furthermore, in A1, the volume fraction of the ethanol aqueous solution is 90-92%.

[0012] Furthermore, in A2, the volume fraction of the ethanol-water solution is 70-75%.

[0013] Furthermore, in both A1 and A2, the heating and reflux conditions are: heating to 72–82°C and refluxing for 2.5–3 hours.

[0014] Furthermore, in both A1 and A2, the particles are pulverized to a particle size of 40–60 mesh.

[0015] Furthermore, in A3, vacuum rotary evaporation is used for concentration, with a vacuum degree of -0.08 MPa and a rotary evaporation temperature of 60°C.

[0016] Furthermore, in A3, the spray drying inlet air temperature is 110–120°C, and the outlet air temperature is 60–75°C.

[0017] Furthermore, the sulfur powder, realgar powder, gamboge powder, and pearl powder are all passed through a 180-mesh sieve.

[0018] Furthermore, the specific sterilization operation is as follows: keep warm at 155-160℃ for 40-60 minutes.

[0019] Secondly, the present invention provides an ointment for treating burns, prepared using the above-described preparation method.

[0020] The relevant efficacy of the Chinese herbal raw materials involved are as follows:

[0021] Sulfur: It has a sour taste and is warm but not drying in nature. It is suitable for treating sores and other diseases; it is also suitable for secondary infections or eczema-like changes after burns; it is often used in combination with other heat-clearing and detoxifying drugs to reduce heat stimulation.

[0022] Realgar: It has a pungent taste and warm properties. It has the effects of drying dampness and eliminating phlegm. It is suitable for diseases such as carbuncles and boils. It is also suitable for burns with local redness, swelling, oozing or mild infection.

[0023] Gamboge: It is cold in nature and has a sour, pungent, and astringent taste. It has the effects of breaking up blood stasis, detoxifying, and stopping bleeding. Modern research shows that it has the effects of clearing heat and detoxifying, reducing swelling and relieving pain. Due to its strong toxicity, it is used externally after processing.

[0024] Pearl: It has detoxifying, anti-corrosion, and tissue-regenerating effects, as well as anti-inflammatory properties and the ability to enhance the repair and regeneration of injured tissues. Pearl powder can accelerate epidermal cell regeneration, promote granulation tissue formation, and reduce scar formation. It is widely used in the later stages of burn and scald repair and also has anti-inflammatory and antioxidant effects.

[0025] Rhubarb: Its main components include anthraquinones (chrysophanic acid, emodin, chrysophanol, etc.), tannins and related compounds, as well as free gallic acid, cinnamic acid and their esters.

[0026] Artemisia argyi: Its main components include volatile oils, flavonoids, eucalyptol, and triterpenoids; it has antibacterial effects and enhances the phagocytic function of reticuloendothelial cells. It helps prevent wound infection and improves microcirculation.

[0027] Scutellaria baicalensis: Its main components include baicalin and baicalin, which have strong anti-inflammatory, antioxidant and matrix metalloproteinase inhibitory effects.

[0028] Coptis chinensis: Its main components include berberine and coptisine; it can inhibit bacteria and viruses, suppress the release of various inflammatory mediators, and quickly relieve redness, swelling, heat and pain. It is a key medicinal material for treating burns complicated with infection.

[0029] Gardenia: Its main components include genipin; it can regulate inflammatory pathways (such as NF-κB), reduce tissue inflammation damage caused by high temperature, and relieve pain. It is often used as an adjunct treatment for moderate to severe burns.

[0030] Dandelion: Clears heat and detoxifies, reduces swelling and dissipates nodules; rich in flavonoids, it can scavenge oxygen free radicals and reduce oxidative stress damage; dandelion sterols have certain anti-inflammatory and analgesic effects, suitable for the early redness and swelling stage of burns, helping to control the development of local inflammation.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The present invention introduces a compound extract in the preparation of an ointment for treating burns, which is prepared by extract one and extract two. Extract one is obtained by reflux extraction of rhubarb, artemisia argyi and scutellaria baicalensis with a very high concentration of ethanol aqueous solution (volume fraction 90-92%), and is the "antibacterial and healing-promoting component". Extract two is obtained by reflux extraction of coptis chinensis, gardenia and dandelion with a medium concentration of ethanol aqueous solution (volume fraction 70-75%), and is the "analgesic component".

[0033] The "antibacterial and healing-promoting component" is extracted from multiple raw materials in an ultra-high concentration ethanol aqueous solution (volume fraction 90-92%). On the one hand, it has a positive effect: the ultra-high concentration ethanol aqueous solution (volume fraction 90-92%) facilitates the extraction of more fat-soluble components (such as eucalyptol from Artemisia argyi and volatile oil from Angelica sinensis), thereby enhancing the antibacterial effect. On the other hand, it also has a negative effect: over-extraction leads to an increase in the dissolution of impurities (such as waxes), which interferes with the stability of other related active ingredients, resulting in a decrease in the healing-promoting effect.

[0034] Meanwhile, the "antibacterial and wound-healing component" contains Scutellaria baicalensis among its many raw materials. Baicalein in Scutellaria baicalensis exhibits surfactant-like properties, helping to reduce the aggregation of waxy impurities produced by high-concentration ethanol aqueous solution extraction, reducing the impurity particle size to the submicron level. This weakens / countersacts the negative impact of high-concentration ethanol aqueous solution reflux extraction on the wound-healing effect. Furthermore, the phenolic hydroxyl groups of baicalein in Scutellaria baicalensis can undergo esterification with free gallic acid in rhubarb to generate novel ester derivatives (such as galloyl baicalein), enhancing antibacterial activity / effect. This synergistically improves the antibacterial properties and wound-healing properties of the prepared ointment. Attached Figure Description

[0035] Figure 1 This is a comparative trend graph showing the change in wound healing rate of scalded rats treated with ointments prepared in Example 1 and Comparative Examples 1 to 4 of the present invention as a function of administration time.

[0036] Figure 2 This is a trend chart comparing the MIC and MBC of the ointments prepared in Examples 1 to 3 and Comparative Examples 1 to 3 of the present invention against Staphylococcus aureus.

[0037] Figure 3 This is a trend chart comparing the MIC and MBC of the ointments prepared in Examples 1 to 3 and Comparative Examples 1 to 3 of the present invention against Escherichia coli. Detailed Implementation

[0038] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0039] Unless otherwise specified, the raw materials used in the embodiments and comparative examples of this application are all commercially available.

[0040] The preparation methods of the ointment for treating burns in Examples 1 to 3 and Comparative Examples 1 to 3 below include the following steps: 58g of sulfur powder and 19g of realgar powder are added to 885g of tung oil, heated to 270℃ and continuously stirred at 270rpm for 32min, then cooled to 158℃; 2.1g of gamboge powder and 1.9g of pearl powder are added, and stirring is continued for 32min, followed by filtration, sterilization at 158℃ for 50min, and cooling to 58℃; 3.4g of compound extract is added, mixed well, and cooled to room temperature to obtain the ointment for treating burns. The sulfur powder, realgar powder, gamboge powder, and pearl powder are all extremely fine powders, passed through an 180-mesh sieve.

[0041] Example 1: The preparation method of the compound extract is as follows:

[0042] A1. By weight, mix 8.2 parts of rhubarb, 6.3 parts of mugwort and 3.3 parts of scutellaria, grind them (40-60 mesh), add them to an ethanol aqueous solution (volume fraction of 91%) that is 8 times their weight, heat to reflux, specifically heat to 73°C, reflux for 2.7 h, filter, and obtain extract one.

[0043] A2. By weight, mix 2.4 parts of Coptis chinensis, 2.1 parts of Gardenia jasminoides and 1.6 parts of Taraxacum mongolicum, pulverize (40-60 mesh), add to 9 times their weight of ethanol aqueous solution (volume fraction of 73%), heat to reflux, specifically heat to 76℃, reflux for 2.7h, filter, and obtain extract two.

[0044] A3. Extract 1 and Extract 2 were concentrated separately under vacuum rotary evaporation to a relative density of 1.12 (measured at 60℃). Specifically, the concentration was achieved under a vacuum of -0.08 MPa and rotary evaporation at 60℃. The two extracts were then mixed to obtain a mixture. 0.5% by mass of a process aid (pharmaceutical grade silica powder AEROSIL® 200Pharma) was added to this mixture and mixed thoroughly. The mixture was then spray-dried using a centrifugal atomizing disc at a speed of 18000 rpm, a particle size D50 of 80–100 μm, an inlet air temperature of 114℃, and an outlet air temperature of 66℃ to obtain the composite extract.

[0045] Example 2: The preparation method of the compound extract is as follows:

[0046] A1. By weight, mix 8 parts rhubarb, 6 parts mugwort, and 3.2 parts scutellaria, grind them (40-60 mesh), add them to an ethanol aqueous solution (90% by volume) that is 8 times their weight, heat to reflux, specifically heat to 73°C, reflux for 2.7 h, filter, and obtain extract one.

[0047] A2. By weight, mix 2.3 parts of Coptis chinensis, 2 parts of Gardenia jasminoides and 1.5 parts of Taraxacum mongolicum, grind them (40-60 mesh), add them to an ethanol aqueous solution (volume fraction of 73%) that is 9 times their weight, heat to reflux, specifically heat to 76°C, reflux for 2.7 h, filter, and obtain extract two.

[0048] A3. Extract 1 and Extract 2 were concentrated to a relative density of 1.12 (measured at 60℃) by vacuum rotary evaporation. Specifically, the extraction was carried out at 60℃ under a vacuum of -0.08MPa. The two were then mixed and spray-dried at an inlet air temperature of 115℃ and an outlet air temperature of 68℃ to obtain the composite extract.

[0049] Example 3: The preparation method of the compound extract is as follows:

[0050] A1. By weight, mix 8.5 parts of rhubarb, 6.5 parts of mugwort and 3.5 parts of scutellaria, grind them (40-60 mesh), add them to an ethanol aqueous solution (volume fraction of 92%) that is 8 times their weight, heat to reflux, specifically heat to 73°C, reflux for 2.7 h, filter, and obtain extract one.

[0051] A2. By weight, mix 2.6 parts of Coptis chinensis, 2.2 parts of Gardenia jasminoides and 1.7 parts of Taraxacum mongolicum, pulverize (40-60 mesh), add to 9 times their weight of ethanol aqueous solution (volume fraction of 75%), heat to reflux, specifically heat to 76℃, reflux for 2.7h, filter, and obtain extract two.

[0052] A3. Extract 1 and Extract 2 were concentrated to a relative density of 1.13 (measured at 60℃) by vacuum rotary evaporation. Specifically, the extraction was carried out at 60℃ under a vacuum of -0.08MPa. The two were then mixed and spray-dried at an inlet air temperature of 115℃ and an outlet air temperature of 66℃ to obtain the composite extract.

[0053] Comparative Example 1: The only difference between this comparative example and Example 1 is that in the preparation of the compound extract, the raw material for the preparation of extract one, Scutellaria baicalensis, is changed to the raw material for the preparation of extract two; and the volume fraction of the ethanol aqueous solution used in the preparation of extract one is reduced to 80%.

[0054] Specifically, the preparation method of the compound extract is as follows:

[0055] A1. By weight, mix 8.2 parts of rhubarb and 6.3 parts of mugwort, pulverize (40-60 mesh), add to an ethanol aqueous solution (80% by volume) with 8 times their weight, heat under reflux, filter, and obtain extract one.

[0056] A2. By weight, mix 2.4 parts of Coptis chinensis, 2.1 parts of Gardenia jasminoides, 1.6 parts of Taraxacum mongolicum and 3.3 parts of Scutellaria baicalensis, pulverize (40-60 mesh), add to 9 times their weight of ethanol aqueous solution (volume fraction of 73%), heat under reflux, filter, and obtain extract two.

[0057] A3. Extract 1 and Extract 2 were concentrated to a relative density of 1.12 (measured at 60℃) by vacuum rotary evaporation, then the two were mixed and spray-dried to obtain the composite extract.

[0058] Comparative Example 2: The only difference between this comparative example and Example 1 is that in the preparation of the compound extract, the raw material for preparing extract one, Scutellaria baicalensis, is replaced with the raw material for preparing extract two.

[0059] Specifically, the preparation method of the compound extract is as follows:

[0060] A1. By weight, mix 8.2 parts of rhubarb and 6.3 parts of mugwort, pulverize (40-60 mesh), add to 8 times their weight of ethanol aqueous solution (volume fraction of 91%), heat under reflux, filter, and obtain extract one.

[0061] A2. By weight, mix 2.4 parts of Coptis chinensis, 2.1 parts of Gardenia jasminoides, 1.6 parts of Taraxacum mongolicum and 3.3 parts of Scutellaria baicalensis, pulverize (40-60 mesh), add to 9 times their weight of ethanol aqueous solution (volume fraction of 73%), heat under reflux, filter, and obtain extract two.

[0062] A3. Extract 1 and Extract 2 were concentrated to a relative density of 1.12 (measured at 60℃) by vacuum rotary evaporation, then the two were mixed and spray-dried to obtain the composite extract.

[0063] Comparative Example 3: The only difference between this comparative example and Example 1 is that the volume fraction of the ethanol aqueous solution used in the preparation of the compound extract is reduced to 80%.

[0064] Specifically, the preparation method of the compound extract is as follows:

[0065] A1. By weight, mix 8.2 parts of rhubarb, 6.3 parts of mugwort and 3.3 parts of scutellaria, grind them (40-60 mesh), add them to an ethanol aqueous solution (80% by volume) with 8 times their weight, heat under reflux, filter, and obtain extract one.

[0066] A2. By weight, mix 2.4 parts of Coptis chinensis, 2.1 parts of Gardenia jasminoides and 1.6 parts of Taraxacum mongolicum, pulverize (40-60 mesh), add to 9 times their weight of ethanol aqueous solution (volume fraction of 73%), heat under reflux, filter, and obtain extract two.

[0067] A3. Extract 1 and Extract 2 were concentrated to a relative density of 1.12 (measured at 60℃) by vacuum rotary evaporation, then the two were mixed and spray-dried to obtain the composite extract.

[0068] Comparative Example 4: The only difference between this comparative example and Example 1 is that artificial musk is added to the preparation of the ointment for treating burns.

[0069] Specifically, the preparation method of the ointment for treating burns includes the following steps: 58g of sulfur powder and 19g of realgar powder are added to 885g of tung oil, heated to 270℃ and continuously stirred at 270rpm for 32min, then cooled to 158℃; 2.1g of gamboge powder and 1.9g of pearl powder are added, and stirring is continued for 32min, then filtered, sterilized by keeping warm at 158℃ for 50min, and cooled to 58℃; 1.4g of artificial musk and 3.4g of compound extract are added, mixed well, and cooled to room temperature to obtain the ointment for treating burns.

[0070] Experimental Example 1: Test subjects: Ointments prepared in Examples 1 to 3 and Comparative Examples 1 to 4.

[0071] Experimental items and methods: Rat burn treatment (wound healing promotion) experiment.

[0072] Sixty-three SD rats weighing 200–220g were used, and their backs were shaved to a length of approximately 36cm using 8% sodium sulfide. 2 Under ether anesthesia, rats were fixed supine on a perforated wooden board, exposing their hairless skin through the holes. The board was then placed in a heated water bath, immersing the exposed back skin in 78°C hot water for 15 seconds. The rats were then removed and dried with clean gauze, thus creating superficial second-degree burns. Seven burned rats were designated as the model group; seven burned rats were designated as the Sanhuang Pearl Ointment group; and the remaining 49 burned rats were designated as the experimental groups (Examples 1-3 and Comparative Examples 1-4), with seven rats in each group. The model group rats were given excipient (tung oil) 0.3g / time, twice daily (superficial second-degree burns heal spontaneously, and tung oil itself promotes healing); the Sanhuang Pearl Ointment group rats were given Sanhuang Pearl Ointment (Lingrui) 0.3g / time, twice daily; and the experimental groups rats were given the corresponding ointment 0.3g / time, twice daily. Apply the medication to the skin twice daily, bandage with clean gauze, and house the rats individually. Administer the medication for 24 consecutive days, recording daily the wound's scab formation, shedding, edema, and infection status. On days 3, 6, 12, 18, and 24, record the average wound area per rat in each group. Trace the burn area onto transparent tracing paper, weigh the tracing, convert the weight to area, and calculate the wound healing rate. Wound healing rate = (initial burn area - area after medication) / original burn area × 100%.

[0073] Experimental results: see Table 1.

[0074] Table 1. Wound healing rate in scalded rats of each group

[0075]

[0076] Experimental Example 2: Test subjects: Ointments prepared in Examples 1 to 3 and Comparative Examples 1 to 3.

[0077] Test items and methods: In vitro antibacterial (Staphylococcus aureus, Escherichia coli) test.

[0078] Referring to the "Principles of In Vitro Antibacterial Testing of Antimicrobial Drugs" in the "Guidelines for Research on New Traditional Chinese Medicines" and the "Handbook for Bacterial Drug Susceptibility Testing," the minimum inhibitory concentration (MIC) was determined using the test tube twofold dilution method, and the minimum bactericidal concentration (MBC) was determined using the agar plate transfection method. The lower the concentration value, the stronger the corresponding in vitro antibacterial ability.

[0079] Experimental results: see Table 2.

[0080] Table 2. MIC and MBC of each test subject against Staphylococcus aureus and Escherichia coli

[0081]

[0082] Results Analysis: Combining the data in Table 1 and Table 2, and Figures 1-3 Analysis of Examples 1 to 3 shows that the ointment prepared by the present invention (Examples 1 to 3) achieved a wound healing rate of over 97.8% (on day 24) in rats with superficial second-degree burns; it also exhibits excellent antibacterial properties, with a MIC as low as 0.03 mg / mL and an MBC as low as 0.06 mg / mL against Staphylococcus aureus; and a MIC as low as 1.95 mg / mL and an MBC as low as 7.81 mg / mL against Escherichia coli.

[0083] Combining the data in Table 1 and Table 2 and Figures 1-3 Analysis was performed on Example 1 and Comparative Examples 1 to 4:

[0084] Specifically, by comparing Comparative Example 1 and Comparative Example 2, it can be seen that in the preparation of the compound extract, the volume fraction of the ethanol aqueous solution used in the preparation of extract one (raw materials are rhubarb and mugwort) increased from a medium-high concentration of 80% (Comparative Example 1) to an ultra-high concentration of 91% (Comparative Example 2). As a result, the MIC and MBC of the prepared ointment against Staphylococcus aureus and against Escherichia coli both decreased, indicating enhanced antibacterial activity. However, the wound healing rate in rats with superficial second-degree burns decreased significantly, indicating reduced wound healing performance.

[0085] This is mainly because when extracting with a medium-to-high concentration ethanol aqueous solution (e.g., 80±2%) under reflux, the polarity is suitable, balancing the extraction of both hydrophilic and lipophilic components. Anthraquinones in rhubarb (such as rhein and emodin) and Artemisia argyi volatile oil (the main antibacterial and healing promoters) can be efficiently dissolved, forming a multi-target synergistic effect. However, when switching to ultra-high concentration ethanol aqueous solution (e.g., >90%) under reflux, on the one hand, there are positive effects: more lipid-soluble components (such as Artemisia argyi eucalyptol and Angelica sinensis volatile oil) are extracted, thus enhancing antibacterial properties; on the other hand, there are negative effects: over-extraction leads to increased dissolution of impurities (such as waxes), interfering with the stability of related active ingredients, and consequently reducing the wound-healing effect.

[0086] Specifically, by comparing Comparative Example 1 and Comparative Example 3, it can be seen that, compared to Comparative Example 1, Comparative Example 3 changed the raw material Scutellaria baicalensis of Extract 2 to be extracted together with Rheum palmatum and Artemisia argyi of Extract 1. As a result, the antibacterial properties of the ointment were enhanced; the wound healing ability did not change significantly.

[0087] In comparison with Example 1, it can be seen that by changing the raw material Scutellaria baicalensis in Extract 2 to extract it together with the raw materials Rheum palmatum and Artemisia argyi in Extract 1, and by increasing the volume fraction of the ethanol aqueous solution used for reflux extraction from a medium-high concentration of 80% to an ultra-high concentration of 91%, the two can produce a synergistic effect, which can synergistically improve the antibacterial and wound healing properties of the prepared ointment, ensuring that the prepared ointment has both excellent antibacterial properties and wound healing promotion effect.

[0088] Comparing Example 1 and Comparative Example 4, it can be seen that compared with Example 1, Comparative Example 4 introduced artificial musk. As a result, the wound healing rate of rats with superficial second-degree burns was significantly reduced in the early stage (6 days after administration). However, the wound healing rate did not change significantly in the final stage (24 days after administration).

[0089] This is mainly because artificial musk has a bitter taste and is slightly cold in nature. Although it has the effect of clearing heat and relieving pain, in the early stage of burns (generally within 48 hours), the focus should be on cooling down and reducing exudation. When artificial musk is used together with other components in this invention, its "blood-activating" properties may aggravate local congestion and edema, delaying the early / initial healing process of burns.

[0090] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for preparing an ointment for treating burns, characterized in that, The process includes the following steps: 57-60g of sulfur powder and 18-20g of realgar powder are added to 880-890g of tung oil. The mixture is heated to 270±2℃ and stirred continuously for 30-35 minutes, then cooled to 155-160℃. 2-2.2g of gamboge powder and 1.8-2g of pearl powder are added, and the mixture is stirred for another 30-35 minutes. The mixture is then filtered, sterilized, and cooled to 55-60℃. 3.2-3.5g of a compound extract is added, mixed well, and cooled to room temperature to obtain the ointment for treating burns. The preparation method of the composite extract is as follows: A1. By weight, mix 8-8.5 parts of rhubarb, 6-6.5 parts of mugwort and 3.2-3.5 parts of scutellaria, crush them, add them to an ethanol aqueous solution with a weight of 7-9 times, heat under reflux, filter, and obtain extract one. A2. By weight, mix 2.3-2.6 parts of Coptis chinensis, 2-2.2 parts of Gardenia jasminoides and 1.5-1.7 parts of Taraxacum mongolicum, crush them, add them to an ethanol aqueous solution with a weight of 8-10 times, heat under reflux, filter, and obtain extract two. A3. Concentrate the first extract and the second extract to a relative density of 1.1 to 1.15 respectively, mix them, and spray dry to obtain the composite extract. In A1, the volume fraction of the ethanol-water solution is 90-92%; In A2, the volume fraction of the ethanol aqueous solution is 70-75%.

2. The method for preparing the ointment for treating burns according to claim 1, characterized in that, In both A1 and A2, the heating and reflux conditions are: heating to 72–82°C and refluxing for 2.5–3 hours.

3. The method for preparing the ointment for treating burns according to claim 1, characterized in that, In both A1 and A2, the particles are pulverized to a size of 40–60 mesh.

4. The method for preparing the ointment for treating burns according to claim 1, characterized in that, In A3, vacuum rotary evaporation is used for concentration.

5. The method for preparing the ointment for treating burns according to claim 1, characterized in that, In A3, the inlet air temperature of the spray dryer is 110-120°C, and the outlet air temperature is 60-75°C.

6. The method for preparing the ointment for treating burns according to claim 1, characterized in that, The sulfur powder, realgar powder, gamboge powder, and pearl powder all passed through a 180-mesh sieve.

7. The method for preparing the ointment for treating burns according to claim 1, characterized in that, The specific sterilization procedure is as follows: keep warm at 155-160℃ for 40-60 minutes.

8. An ointment for treating burns, characterized in that, It is prepared by the preparation method according to any one of claims 1 to 7.

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