Tobacco leaf surface fertilizer for improving oil content and anti-epidemic ability of tobacco leaves and preparation method thereof

Abstract

The present application belongs to the technical field of tobacco leaf fertilizer, and provides a tobacco leaf fertilizer for improving oil content and anti-epidemic ability of tobacco leaves, which comprises water and active ingredients, wherein the active ingredients comprise the following components in parts by weight: 240-300 parts of sodium humate, 40-60 parts of potassium nitrate, 15-30 parts of magnesium sulfate heptahydrate, 1-2 parts of striga lactone, 5-8 parts of N-succinylated chitosan, and 1000-1400 parts of tobacco stem enzymolysis fermentation product. The leaf fertilizer can significantly improve the oil content of tobacco leaves, and the required content of active ingredients is low when the leaf fertilizer is applied, thereby reducing the application cost. Meanwhile, the leaf fertilizer can also significantly improve the disease control ability of tobacco, and has good popularization and application value.

Description

Technical Field

[0001] This invention belongs to the field of tobacco foliar fertilizer technology, specifically relating to a tobacco foliar fertilizer that improves the oil content and disease resistance of tobacco leaves and its preparation method. Background Technology

[0002] Because chemical fertilizers can easily cause soil compaction, salinization, and water and soil pollution, in recent years, fertilizer research has increasingly focused on organic fertilizers and microbial fertilizers. New types of foliar fertilizers are also a key area of ​​research.

[0003] Currently, research on foliar fertilizers mainly focuses on conventional nitrogen, phosphorus, potassium, and trace elements. While existing technologies have yielded some products with good fertilizer efficacy, very little research has been conducted on increasing the oil content of tobacco leaves. Furthermore, existing foliar fertilizers have numerous components, are complex to formulate, and have limited utilization of tobacco planting byproducts. Therefore, developing novel tobacco foliar fertilizers that address these issues is a worthy research topic. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the purpose of this invention is to provide a tobacco foliar fertilizer that can significantly increase the oil content of tobacco leaves, improve the tobacco's ability to resist disease, and has a simple composition.

[0005] This invention provides the following technical solution:

[0006] A tobacco foliar fertilizer for improving the oil content and disease resistance of tobacco leaves, the tobacco foliar fertilizer comprising water and active ingredients, the active ingredients comprising the following components by weight:

[0007] 240-300 parts sodium humate, 40-60 parts potassium nitrate, 15-30 parts magnesium sulfate heptahydrate, 1-2 parts strigolactone, 5-8 parts N-succinylated chitosan, 1000-1400 parts enzymatic hydrolysis fermentation product of tobacco stems;

[0008] The tobacco stem enzymatic hydrolysis fermentation product is obtained by enzymatically hydrolyzing tobacco stems with a compound enzyme, then fermenting the liquid-phase dried powder with chicken manure and drying it; the compound enzyme is composed of cellulose, hemicellulase and laccase in an enzyme activity ratio of 6:10:2~4.

[0009] Preferably, when using a compound enzyme for enzymatic hydrolysis, the hydrolysis temperature is 28°C and the hydrolysis time is 1 hour.

[0010] Preferably, after the enzymatic hydrolysis, the hydrolysate is freeze-dried, then mixed with chicken manure at a weight ratio of 1:1 to 3, and subjected to natural composting fermentation.

[0011] Preferably, the fermentation time during the natural composting fermentation is 15 days.

[0012] Preferably, during the natural composting fermentation, the initial water content of the fermentation substrate is 30%wt.

[0013] Preferably, when preparing the tobacco stem enzymatic hydrolysate fermentation product, the drying process is carried out at 40°C.

[0014] Preferably, the complex enzyme is composed of cellulose, hemicellulase and laccase in an enzyme activity unit ratio of 6:10:3.

[0015] Preferably, the active ingredient comprises the following components in parts by weight:

[0016] 250 parts sodium humate, 45 parts potassium nitrate, 20 parts magnesium sulfate heptahydrate, 1 part strigolactone, 8 parts N-succinylated chitosan, and 1200 parts enzymatic hydrolysis fermentation product of tobacco stems.

[0017] Preferably, the weight ratio of water to active ingredient is 10000~1000:1.

[0018] A method for preparing a tobacco foliar fertilizer that enhances the oil content and disease resistance of tobacco leaves, wherein the preparation method involves dissolving the active ingredients in the aforementioned tobacco foliar fertilizer in water.

[0019] The beneficial effects of this invention are:

[0020] This invention discovers a synergistic effect between enzymatic hydrolysis and fermentation products of tobacco stems and strigolactones in increasing the oil content of tobacco leaves. Based on this, a foliar fertilizer was developed that significantly increases the oil content of tobacco leaves compared to conventional water-soluble foliar fertilizers. This foliar fertilizer requires less active ingredient and has low application costs. Furthermore, this foliar fertilizer can significantly enhance the tobacco's ability to control diseases, making it valuable for widespread application. Detailed Implementation

[0021] The present invention will be specifically described below through embodiments. It should be noted that the following embodiments are only used to further illustrate the present invention and should not be construed as limiting the scope of protection of the present invention. Some non-essential improvements and adjustments made by those skilled in the art based on the above-described invention are still within the scope of protection of the present invention.

[0022] Example 1

[0023] 1. Raw materials:

[0024] Sodium humate: purchased from Shanxi Jiayou Humic Acid Technology Co., Ltd.;

[0025] Potassium nitrate: purchased from Sichuan Rongzun Yida Agricultural Technology Co., Ltd.

[0026] Magnesium sulfate heptahydrate: purchased from Guangzhou Ruifeng New Chemical Technology Co., Ltd.;

[0027] strigolactone: purchased from Hubei Biaoyue Biotechnology Development Co., Ltd.;

[0028] N-succinylated chitosan: purchased from Xi'an Tianguangyuan Biotechnology Co., Ltd.;

[0029] Chitosan: Purchased from Anhui Xinsheng Biotechnology Co., Ltd.;

[0030] Cellulase, hemicellulase, and laccase: purchased from Jiangsu Ruiduo Bioengineering Co., Ltd.

[0031] Tobacco stems: donated to local tobacco farmers.

[0032] 2. Preparation method

[0033] 2.1 Preparation of enzymatically hydrolyzed tobacco stem powder: The tobacco stems were crushed, passed through a 100-mesh sieve, and immersed in a complex enzyme aqueous solution composed of cellulase, hemicellulase, and laccase in an enzyme activity unit ratio of 6:10:3, wherein the amount of cellulase added was 10 FPU per gram of tobacco stem; after mixing evenly, the mixture was enzymatically hydrolyzed at 28°C for 1 hour; after centrifugation, the enzymatic hydrolysate was collected and then freeze-dried into powder, which was designated as enzymatically hydrolyzed tobacco stem powder.

[0034] 2.2 Preparation of enzymatically hydrolyzed tobacco stem fermentation product: Enzymatically hydrolyzed tobacco stem powder was prepared according to the aforementioned method for preparing enzymatically hydrolyzed tobacco stem. The enzymatically hydrolyzed tobacco stem powder was mixed with chicken manure at a weight ratio of 1:3, and the water content was controlled at 30%wt. The mixture was then naturally composted for 15 days, dried, and ground to obtain powder.

[0035] 2.3 Prepare the following raw materials according to parts by weight:

[0036] 250 parts sodium humate, 45 parts potassium nitrate, 20 parts magnesium sulfate heptahydrate, 1 part strigolactone, 8 parts N-succinylated chitosan, and 1200 parts enzymatic hydrolysis fermentation product of tobacco stems.

[0037] 2.4 Using the raw material composition obtained in 2.3 as the active ingredient, prepare 1000 parts by weight of water and 1 part by weight of the active ingredient, and dissolve the active ingredient in the water to obtain the foliar fertilizer.

[0038] Example 2

[0039] 1. Raw materials:

[0040] Sodium humate: purchased from Shanxi Jiayou Humic Acid Technology Co., Ltd.;

[0041] Potassium nitrate: purchased from Sichuan Rongzun Yida Agricultural Technology Co., Ltd.

[0042] Magnesium sulfate heptahydrate: purchased from Guangzhou Ruifeng New Chemical Technology Co., Ltd.;

[0043] strigolactone: purchased from Hubei Biaoyue Biotechnology Development Co., Ltd.;

[0044] O-succinylated chitosan: purchased from Xi'an Tianguangyuan Biotechnology Co., Ltd.;

[0045] Chitosan: Purchased from Anhui Xinsheng Biotechnology Co., Ltd.;

[0046] Cellulase, hemicellulase, and laccase: purchased from Jiangsu Ruiduo Bioengineering Co., Ltd.

[0047] Tobacco stems: donated to local tobacco farmers.

[0048] 2. Preparation method

[0049] 2.1 Preparation of enzymatically hydrolyzed tobacco stem powder: The tobacco stems were crushed, passed through a 100-mesh sieve, and immersed in a complex enzyme aqueous solution composed of cellulase, hemicellulase, and laccase in an enzyme activity unit ratio of 6:10:4, wherein the amount of cellulase added was 10 FPU per gram of tobacco stem; after mixing evenly, the mixture was enzymatically hydrolyzed at 28°C for 1 hour; after centrifugation, the enzymatic hydrolysate was collected and then freeze-dried into powder, which was designated as enzymatically hydrolyzed tobacco stem powder.

[0050] 2.2 Preparation of enzymatically hydrolyzed tobacco stem fermentation product: Enzymatically hydrolyzed tobacco stem powder was prepared according to the aforementioned method for preparing enzymatically hydrolyzed tobacco stem. The enzymatically hydrolyzed tobacco stem powder was mixed with chicken manure at a weight ratio of 1:3, and the water content was controlled at 30%wt. The mixture was then naturally composted for 15 days, dried, and ground to obtain powder.

[0051] 2.3 Prepare the following raw materials according to parts by weight:

[0052] 300 parts sodium humate, 60 parts potassium nitrate, 30 parts magnesium sulfate heptahydrate, 2 parts strigolactone, 5 parts N-succinylated chitosan, and 1400 parts enzymatic hydrolysis fermentation product of tobacco stems.

[0053] 2.4 Using the raw material composition obtained in 2.3 as the active ingredient, prepare 3000 parts by weight of water and 1 part by weight of the active ingredient, and dissolve the active ingredient in the water to obtain the foliar fertilizer.

[0054] Example 3

[0055] 1. Raw materials:

[0056] Sodium humate: purchased from Shanxi Jiayou Humic Acid Technology Co., Ltd.;

[0057] Potassium nitrate: purchased from Sichuan Rongzun Yida Agricultural Technology Co., Ltd.

[0058] Magnesium sulfate heptahydrate: purchased from Guangzhou Ruifeng New Chemical Technology Co., Ltd.;

[0059] strigolactone: purchased from Hubei Biaoyue Biotechnology Development Co., Ltd.;

[0060] P-succinylated chitosan: purchased from Xi'an Tianguangyuan Biotechnology Co., Ltd.;

[0061] Chitosan: Purchased from Anhui Xinsheng Biotechnology Co., Ltd.;

[0062] Cellulase, hemicellulase, and laccase: purchased from Jiangsu Ruiduo Bioengineering Co., Ltd.

[0063] Tobacco stems: donated to local tobacco farmers.

[0064] 2. Preparation method

[0065] 2.1 Preparation of enzymatically hydrolyzed tobacco stem powder: The tobacco stems were crushed, passed through a 100-mesh sieve, and immersed in a complex enzyme aqueous solution composed of cellulase, hemicellulase, and laccase in an enzyme activity unit ratio of 6:10:2, wherein the amount of cellulase added was 10 FPU per gram of tobacco stem; after mixing evenly, the mixture was enzymatically hydrolyzed at 28°C for 1 hour; after centrifugation, the enzymatic hydrolysate was collected and then freeze-dried into powder, which was designated as enzymatically hydrolyzed tobacco stem powder.

[0066] 2.2 Preparation of enzymatically hydrolyzed tobacco stem fermentation product: Enzymatically hydrolyzed tobacco stem powder was prepared according to the aforementioned method for preparing enzymatically hydrolyzed tobacco stem. The enzymatically hydrolyzed tobacco stem powder was mixed with chicken manure at a weight ratio of 1:3, and the water content was controlled at 30%wt. The mixture was then naturally composted for 15 days, dried, and ground to obtain powder.

[0067] 2.3 Prepare the following raw materials according to parts by weight:

[0068] 240 parts sodium humate, 40 parts potassium nitrate, 15 parts magnesium sulfate heptahydrate, 1.5 parts strigolactone, 6 parts N-succinylated chitosan, and 1000 parts enzymatic hydrolysis fermentation product of tobacco stems.

[0069] 2.4 Using the raw material composition obtained in 2.3 as the active ingredient, prepare 10,000 parts by weight of water and 1 part by weight of the active ingredient, and dissolve the active ingredient in the water to obtain the foliar fertilizer.

[0070] Comparative Example 1

[0071] Compared with Example 1, except that sodium humate was not added, everything else was the same as Example 1.

[0072] Comparative Example 2

[0073] Compared with Example 1, except that N-succinylated chitosan was not added, everything else was the same as Example 1.

[0074] Comparative Example 3

[0075] Compared with Example 1, except that N-succinylated chitosan was replaced with chitosan, everything else was the same as in Example 1.

[0076] Comparative Example 4

[0077] Compared with Example 1, except that the weight of N-succinylated chitosan is 1 part, everything else is the same as in Example 1.

[0078] Comparative Example 5

[0079] Compared with Example 1, except that the weight parts of potassium nitrate are 10 parts and the weight parts of magnesium sulfate heptahydrate are 5 parts, the rest are the same as Example 1.

[0080] Preliminary Experiment Example 1

[0081] Plant hormones are commonly used in crop cultivation as exogenous substances to promote crop growth, mainly including auxins, gibberellins, and cytokinins. Strigolactones, a collective term for strigolactones, are a novel type of plant hormone. Currently, research on their application in tobacco cultivation is relatively limited; existing studies mainly focus on the effects of strigolactones on the growth of tobacco axillary buds. [1-2] To the inventor's knowledge, no one has yet studied the effects of strigolactones on tobacco oil content.

[0082] The inventors considered adding it to conventional tobacco water-soluble foliar fertilizer ("Miliqi" tobacco-specific water-soluble fertilizer, product number: ALD-HGZY, purchased from Jining Alida Biotechnology Co., Ltd.) to observe its effect on tobacco oil content. The experimental site was a tobacco planting base in Qujing City, Yunnan Province, and the variety was Yunyan 87. A control group was sprayed with only conventional tobacco water-soluble foliar fertilizer, while the experimental group was sprayed with conventional tobacco water-soluble foliar fertilizer supplemented with strigolactone (0.001% wt). Spraying was performed once each at the crowning stage, vigorous growth stage, and dome stage, with the criterion being that the leaves were wet but not dripping after spraying. References [3] The oil content of tobacco was determined by ultrasound-assisted Soxhlet extraction, and the results are shown in Table 1. The experimental results indicate that adding strigolactone to conventional water-soluble foliar fertilizer for tobacco has virtually no effect on the oil content of tobacco leaves.

[0083] Table 1

[0084]

[0085] Preliminary Experiment Example 2

[0086] Tobacco stems, a byproduct of the cigarette industry, account for approximately 20-30% of the total weight of tobacco leaves. They are typically treated as waste, with current main treatment methods including fermentation for fertilizer production. [4] It has been found to have good effects on many crops. [5] However, there is relatively little research on preparing recyclable tobacco fertilizer from tobacco stems, and to the inventor's knowledge, no promising research results have been achieved in this area.

[0087] The inventor has long focused on the research and development of tobacco foliar fertilizers, considering using tobacco stems as raw materials and preparing foliar fertilizers suitable for increasing the oil content of tobacco leaves through enzymatic hydrolysis and fermentation.

[0088] Preparation of enzymatically hydrolyzed tobacco stem powder: The tobacco stems were crushed, passed through a 100-mesh sieve, and immersed in a complex enzyme aqueous solution composed of cellulase, hemicellulase, and laccase in an enzyme activity unit ratio of 6:10:3, wherein the amount of cellulase added was 10 FPU per gram of tobacco stem; after mixing evenly, the mixture was enzymatically hydrolyzed at 28°C for 1 hour; after centrifugation, the enzymatic hydrolysate was collected and then freeze-dried into powder, which was designated as enzymatically hydrolyzed tobacco stem powder.

[0089] Preparation of fermented tobacco stem powder: After crushing the tobacco stems through a 100-mesh sieve, mix them thoroughly with chicken manure at a ratio of 1:3, allow them to ferment naturally for 15 days, and then dry and grind them to obtain powder.

[0090] Preparation of enzymatically hydrolyzed tobacco stem fermentation product: Enzymatically hydrolyzed tobacco stem powder was prepared according to the aforementioned method for preparing enzymatically hydrolyzed tobacco stem. The enzymatically hydrolyzed tobacco stem powder was mixed with chicken manure at a weight ratio of 1:3, and the water content was controlled at 30%wt. The mixture was then naturally composted for 15 days, dried, and ground to obtain powder.

[0091] The aforementioned enzymatically hydrolyzed tobacco stem powder, fermented tobacco stem powder, enzymatically hydrolyzed tobacco stem fermentation product, and strigolactone were added as additives to the conventional tobacco water-soluble foliar fertilizer described in Preliminary Experiment Example 1 to investigate its effects on the oil content of tobacco leaves. The tobacco planting method was the same as in Preliminary Experiment Example 1. The experimental groups and results are shown in Tables 2 and 3.

[0092] As the experimental results show, adding enzymatically hydrolyzed tobacco stem powder, fermented tobacco stem powder, or enzymatically hydrolyzed tobacco stem product alone had no significant effect on the oil content of tobacco leaves. However, the simultaneous addition of enzymatically hydrolyzed tobacco stem product and strigolactone significantly increased the oil content of tobacco leaves. This indicates that enzymatically hydrolyzed tobacco stem product and strigolactone have a synergistic effect in increasing the oil content of tobacco leaves. Encouraged by this research, the inventors conducted a series of exploratory studies, aiming to develop a tobacco foliar fertilizer with a proprietary formula based on this discovery, as detailed in the experimental examples.

[0093] Table 2

[0094]

[0095] Note: "-" indicates that the component was not added, and "1%wt" and "0.001%wt" indicate that it was added to a conventional tobacco water-soluble foliar fertilizer at that weight fraction.

[0096] Table 3

[0097]

[0098] Note: The groups in this table correspond to those in Table 2. The control group consists of those who only received conventional tobacco water-soluble foliar fertilizer.

[0099] Experimental Example

[0100] Based on preliminary experimental example 2, the inventors conducted research on their own foliar fertilizer formulation. In the field of crop foliar fertilizers, humic acid or sodium humate are common raw materials, and there are also reports of their use in tobacco cultivation. [6] Potassium and magnesium salts are also common substances in the formulation of water-soluble fertilizers (e.g., Chinese patent ZL.201310350444.3, entitled "Tobacco Foliar Fertilizer"). However, existing technologies generally require the addition of many substances when formulating water-soluble fertilizers, usually more than 10. Based on identifying tobacco stem enzymatic hydrolysis fermentation products and strigolactones as raw materials, the inventors screened existing common raw materials and surprisingly discovered that, in addition to the aforementioned two raw materials, only four more raw materials need to be added—sodium humate, potassium nitrate, magnesium sulfate heptahydrate, and N-succinylated chitosan—to formulate a foliar fertilizer that can significantly increase the oil content of tobacco leaves.

[0101] The experimental method was the same as in Preliminary Experiment Example 1. As shown in Table 4, the formulation of this invention has a significant impact on the effect of foliar fertilizer. Removing or altering the proportions of any component results in substantial differences in the technical effect. Notably, replacing N-succinylated chitosan with ordinary chitosan also produces a significant difference in the technical effect. This indicates that N-succinylated chitosan, as a biostimulant, interacts better with enzymatic fermentation products of tobacco stems and strigolactones compared to regular chitosan.

[0102] Table 4

[0103]

[0104] Note: The control group was treated with only conventional tobacco water-soluble foliar fertilizer.

[0105] During the experiment on the oil content of tobacco leaves, the inventors also investigated the effects of foliar fertilizer on tobacco blight. This experimental case examines the situation of tobacco target spot disease.

[0106] Disease severity grading criteria:

[0107] Level 0: No disease;

[0108] Grade 1: The area of ​​lesions accounts for less than 1% of the total leaf area;

[0109] Grade 3: 1% ≤ lesion area < 10% of total leaf area;

[0110] Level 5: 10% ≤ the area of ​​lesions accounts for less than 30% of the total leaf area;

[0111] Level 7: 30% ≤ the area of ​​lesions accounts for less than 50% of the total leaf area;

[0112] Level 9: 50% or less of the area of ​​lesions is the total area of ​​the leaf.

[0113] Incidence rate (%) = 100 × Number of diseased leaves / Total number of leaves surveyed;

[0114] Efficacy (%) = 100 × (Control incidence rate - Treatment incidence rate) / Control incidence rate.

[0115] The experimental results are shown in Table 5. The results indicate that the foliar fertilizer of this invention exhibits a significant positive correlation in increasing the oil content of tobacco leaves and improving tobacco resistance to target spot disease.

[0116] Table 5

[0117]

[0118] Note: "-" indicates that there is no preventive effect compared to the control group.

[0119] [1] Fan Wuwei. Effects of strigolactone on dormancy and lateral branch growth of tobacco axillary buds and transcriptome study [D]. Guizhou University, 2021. DOI:10.27047 / d.cnki.ggudu.2021.002782.

[0120] [2] Fan Wuwei, Xiao Zhiwen, Pan Zhiyan, et al. Effects of strigolactone and abscisic acid on axillary bud growth and major chemical components of tobacco leaves [J]. Journal of Mountain Agriculture and Biology, 2022, 41(04):49-53. DOI:10.15958 / j.cnki.sdnyswxb.2022.04.007.

[0121] [3] Li Guofu. Research on the material basis of tobacco oil [D]. Zhengzhou: Zhengzhou University, 2015.

[0122] [4] Gao Ming, Guo Lingyan, Xi Yu, et al. Production of organic fertilizer by bio-fermentation of tobacco stems [J]. Tobacco Science and Technology, 2010, (12): 57-60+65. DOI:CNKI:SUN:YCKJ.0.2010-12-013.

[0123] [5] Lu Xian, Lu Rong, Wang Tiyan, et al. Effects of tobacco stalk organic fertilizer on lettuce yield [J]. Modern Agricultural Science and Technology, 2020, (19): 59-62+65. DOI:CNKI:SUN:ANHE.0.2020-19-021.

[0124] [6] Cui Buyun, Yang Haoyu, Wei Fengjie, et al. Effects of foliar fertilizers containing different concentrations of humic acid on tobacco growth and tobacco quality [J]. Jiangsu Agricultural Sciences, 2018, 46(10):86-88+137. DOI:10.15889 / j.issn.1002-1302.2018.10.022.

Claims

1. A tobacco foliar fertilizer that enhances the oil content and disease resistance of tobacco leaves, characterized in that, The tobacco foliar fertilizer comprises water and an active ingredient, wherein the active ingredient comprises the following components by weight: 240-300 parts sodium humate, 40-60 parts potassium nitrate, 15-30 parts magnesium sulfate heptahydrate, 1-2 parts strigolactone, 5-8 parts N-succinylated chitosan, 1000-1400 parts enzymatic hydrolysis fermentation product of tobacco stems; The tobacco stem enzymatic hydrolysis fermentation product is obtained by enzymatically hydrolyzing tobacco stems with a compound enzyme, then fermenting the liquid-phase dried powder with chicken manure and drying it; the compound enzyme is composed of cellulose, hemicellulase and laccase in an enzyme activity ratio of 6:10:2~4.

2. The tobacco foliar fertilizer according to claim 1, characterized in that, When using a compound enzyme for enzymatic hydrolysis, the hydrolysis temperature is 28℃ and the hydrolysis time is 1 hour.

3. The tobacco foliar fertilizer according to claim 1 or 2, characterized in that, After the enzymatic hydrolysis, the hydrolysate is freeze-dried and then mixed with chicken manure at a weight ratio of 1:1 to 3, and then naturally composted.

4. The tobacco foliar fertilizer according to claim 3, characterized in that, The natural composting fermentation process takes 15 days.

5. The tobacco foliar fertilizer according to claim 4, characterized in that, During the natural composting fermentation, the initial water content of the fermentation substrate was 30%wt.

6. The tobacco foliar fertilizer according to claim 1, 2, 4 or 5, characterized in that, When preparing the enzymatic hydrolysate of the tobacco stem, the drying process is carried out at 40°C.

7. The tobacco foliar fertilizer according to claim 6, characterized in that, The complex enzyme is composed of cellulose, hemicellulase, and laccase in an enzyme activity ratio of 6:10:

3.

8. The tobacco foliar fertilizer according to claim 1 or 7, characterized in that, The active ingredient comprises the following components in parts by weight: 250 parts sodium humate, 45 parts potassium nitrate, 20 parts magnesium sulfate heptahydrate, 1 part strigolactone, 8 parts N-succinylated chitosan, and 1200 parts enzymatic hydrolysis fermentation product of tobacco stems.

9. The tobacco foliar fertilizer according to claim 8, characterized in that, The weight ratio of water to active ingredient is 10000~1000:

1.

10. A method for preparing a tobacco foliar fertilizer that enhances the oil content and disease resistance of tobacco leaves, characterized in that, The preparation method involves dissolving the active ingredient in the tobacco foliar fertilizer according to any one of claims 1 to 9 in the water.

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