A composition with ultraviolet radiation protection function and its preparation method and application

Abstract

The present application relates to a kind of compositions with the function of preventing ultraviolet radiation and its preparation method and application, belong to the field of cosmetics, solve the existing low multiples sunscreen product needs to add sufficient sunscreen, resulting in product skin feel thick and heavy, sticky, prone to whitening, kneading mud phenomenon, and because of sunscreen, cannot be realized with the synergism of "light + protection" of essence liquid and other efficacy products, difficult to meet the consumer's use experience demand of "lightweight sunscreen".According to mass percentage content, the composition with the function of preventing ultraviolet radiation of the present application, main active ingredients include trehalose 0.1%-5%, allantoin 0.01%-1%, betaine 0.1%-5%, panthenol 0.1%-1%, nicotinamide 0.1%-5%, glycyrrhizic acid dipotassium 0.01%-1%, 4-hydroxyethyl piperazine ethanesulfonic acid 0.1%-5%, 5D-fullerene 0.05%-5%, ergothioneine pine extract 0.1%-10% and glabridin 0.001%-0.1%.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a composition with anti-ultraviolet radiation function, its preparation method and application. Background Technology

[0002] In the current cosmetics market, although serums, as an important part of skincare products, perform well in moisturizing and nourishing, they generally have significant shortcomings in sun protection. Traditional sunscreens mainly achieve their effect by adding sunscreen agents; however, this method has a series of undeniable drawbacks and limitations.

[0003] For low-SPF sun protection needs such as daily commutes and indoor window areas, traditional sunscreens still need to add sufficient sunscreen agents, resulting in a thick and sticky feel on the skin, and are prone to whitening and pilling. In addition, due to the presence of sunscreen agents, they cannot achieve the synergy of "lightweight + protection" with functional products such as serums, making it difficult to meet consumers' demand for a "lightweight sun protection" experience. Summary of the Invention

[0004] Based on the above analysis, the present invention aims to provide a composition with anti-ultraviolet radiation function, its preparation method and application, in order to solve the problems of existing low-SPF sunscreen products requiring the addition of sufficient sunscreen agents, resulting in a heavy and sticky feel on the skin, easy whitening and pilling, and the inability to achieve the synergistic effect of "lightweight + protection" with functional products such as serums due to the presence of sunscreen agents, thus failing to meet consumers' demand for "lightweight sunscreen" in terms of user experience.

[0005] The objective of this invention is mainly achieved through the following technical solutions: In a first aspect, the present invention provides a composition with anti-ultraviolet radiation function, wherein, by mass percentage, the main active ingredients include trehalose 0.1%-5%, allantoin 0.01%-1%, betaine 0.1%-5%, panthenol 0.1%-1%, nicotinamide 0.1%-5%, dipotassium glycyrrhizate 0.01%-1%, 4-hydroxyethylpiperazine ethanesulfonic acid 0.1%-5%, 5D-fullerene 0.05%-5%, ergothioneine pine mushroom extract 0.1%-10%, and glycyrrhizin 0.001%-0.1%.

[0006] Preferably, the main active ingredients, by mass percentage, include trehalose 0.1%-2%, allantoin 0.05%-0.3%, betaine 0.1%-3%, panthenol 0.3%-0.8%, nicotinamide 1%-5%, dipotassium glycyrrhizate 0.1%-0.3%, 4-hydroxyethylpiperazine ethanesulfonic acid 0.1%-0.7%, 5D-fullerene 1%-4%, ergothioneine pine mushroom extract 0.5%-5%, and glycyrrhizin 0.008%-0.3%.

[0007] Secondly, the present invention provides a fullerene essence comprising the above-mentioned composition, and further comprising, by weight percentage: 0.01%-0.2% EDTA-2Na, 1%-5% glyceryl polyether-26, 0.01%-2% xanthan gum, 0.06%-2% carbomer, 0.5%-10% 1,3-butanediol, 0.1%-0.6% p-hydroxyacetophenone, 0.1%-2% 1,2-hexanediol, 0.1%-2.5% triethanolamine, 0.1%-5% PEG / PPG-17 / 6 copolymer, 0.001%-5% solubilizer, 0.001%-0.5% fragrance, 0.01%-2% β-glucan, with the balance being deionized water.

[0008] Optionally, its average SPF value is 9.39-11.48.

[0009] Thirdly, the present invention provides a method for preparing a fullerene essence, comprising the following steps: Step 1: Weigh each raw material according to the formula, mix the raw materials in groups to obtain the prepared phases A, B, C, D and E; Step 2: Heat, homogenize and / or dissolve the phase obtained in Step 1; Step 3: Mix the phases to obtain fullerene essence.

[0010] Optionally, in step 1, preparing phase A includes preparing phase A1 and phase A2; The preparation of phase A1 includes: weighing xanthan gum, carbomer, and glycerol polyether-26, mixing them to obtain phase A1; The preparation of phase A2 involves mixing deionized water, trehalose, EDTA-2Na, allantoin, and betaine to obtain phase A2.

[0011] Optionally, in step 1, the preparation of phase C includes: weighing 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone, and glycyrrhizin, mixing them, and obtaining phase C.

[0012] Optionally, the preparation of phase D includes: weighing 5D-fullerene, ergothioneine pine mushroom extract, nicotinamide, dipotassium glycyrrhizate, 4-hydroxyethylpiperazine ethanesulfonic acid, panthenol, β-glucan, PEG / PPG-17 / 6 copolymer, and deionized water, and mixing them to obtain phase D.

[0013] Optionally, in step 2, heating, homogenizing, and / or dissolving the phase obtained in step 1 includes the following steps: Step 2-1: Mix phase A1 and phase A2, heat in a water bath until the components dissolve, stir, and obtain the treated phase A; Step 2-2: Heat phase B and phase C separately in a water bath until the components dissolve, to obtain dissolved phase B and phase C.

[0014] Fourthly, the present invention provides the use of the above-described composition in the preparation of a UV-protective serum.

[0015] Compared with the prior art, the present invention can achieve at least one of the following beneficial effects: (1) By optimizing the components and their contents, this invention identified niacinamide and ergothioneine pine mushroom extract as active ingredients that synergize well with fullerene. The synergistic effect of fullerene, niacinamide, and ergothioneine pine mushroom extract enhances the product's UV protection capability. Specifically, the average SPF value of the serum samples from this invention is 9.39-11.48, which is sufficient to meet consumers' demand for "lightweight sun protection" as a low-SPF sunscreen.

[0016] (2) The essence of the present invention can replace chemical sunscreens with safety hazards, thereby reducing the safety hazard factor and obtaining sunscreen products with high sun protection factor, mild formula, low safety hazard or even environmentally friendly.

[0017] (3) The fullerene essence of the present invention can form a strong protective barrier on the skin to resist photodamage. It is a fullerene essence with high UVB protection, gentle and non-irritating properties, easy absorption and good stability. Compared with traditional chemical or physical sunscreen ingredients, the fullerene essence of the present invention has unparalleled advantages in the field of sunscreen skincare products due to its low irritation, high biocompatibility and excellent sun protection effect.

[0018] (4) The preparation method of the present invention can further ensure the synergistic effect of each component in the prepared essence and improve the product’s UV protection capability.

[0019] In this invention, the above-described technical solutions can be combined with each other to achieve more preferred combinations. Other features and advantages of this invention will be set forth in the following description, and some advantages will become apparent from the description or may be learned by practicing the invention. Attached Figure Description

[0020] The accompanying drawings are for illustrative purposes only and are not intended to limit the invention. Throughout the drawings, the same reference numerals denote the same parts.

[0021] Figure 1 This is a photographic record of the skin on the back of a mouse in the in vivo safety study of the product of Example 2 of the present invention; Figure 2 For the in vivo safety study of the product of Example 2 of this invention, the main skin tissue pathological analysis of mice was performed. Figure 3 This is a mouse blood biochemistry study conducted as part of the in vivo safety study of the product in Example 2 of the present invention. Figure 4 This is a routine blood test in mice used in the in vivo safety study of the product of Example 2 of the present invention. Figure 5 For the in vivo safety study of the product of Example 2 of this invention, the pathological analysis of the major organs of mice was performed. Figure 6 The weight change of mice over 21 days in the in vivo safety study of the product of Example 2 of this invention; Figure 7 The evaluation of the UVB radiation protection performance of mouse skin during the in vivo verification of the UVB protection effect of the product in Example 2 of this invention (photo recording); Figure 8 The evaluation of the UVB radiation protection performance of mouse skin in the in vivo verification of the UVB protection effect of the product in Example 2 of the present invention (histopathological analysis, H&E staining). Figure 9 Evaluation of the UVB radiation protection performance of mouse skin in the in vivo verification of the UVB protection effect of the product of this invention (histopathological analysis, Masson staining); Figure 10 The transmittance of ultraviolet light during the in vitro verification of the UVB protection effect of the product in Example 2 of this invention and the products in Comparative Examples 1-5. Detailed Implementation

[0022] Preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings, which constitute a part of the present invention and, together with the embodiments, serve to illustrate the principles of the invention, but are not intended to limit the scope of the invention. Unless otherwise specified, the methods described are conventional methods. Unless otherwise specified, the raw materials are all available from commercially available sources.

[0023] Fullerenes, a special spherical molecular structure composed of carbon atoms, possess unique physical and chemical properties that endow them with excellent antioxidant and photostability. However, in-depth research has revealed that while fullerenes do absorb and scatter ultraviolet (UV) radiation, their absorption and scattering capabilities are limited, rendering them unsuitable for use as sunscreens.

[0024] It should be noted that niacinamide is used as a whitening agent in cosmetics, and ergothioneine pine mushroom extract is used as an antioxidant. This invention, through optimization of components and content, identified niacinamide and ergothioneine pine mushroom extract as active ingredients that synergize well with fullerene. The synergistic effect of fullerene, niacinamide, and ergothioneine pine mushroom extract enhances the product's UV protection capability, making it suitable as a low-SPF sunscreen to meet consumers' demand for "lightweight sunscreen." This invention's product can replace chemical sunscreens with potential safety hazards, thereby reducing the safety risk factor and achieving a sunscreen product with a high SPF, gentle formula, low safety risks, and even environmental friendliness. The fullerene essence of this invention can form a strong protective barrier on the skin to resist photodamage. It is a fullerene essence with high UVB protection, gentle and non-irritating properties, easy absorption, and good stability. Compared to traditional chemical or physical sunscreen ingredients, the fullerene essence of this invention exhibits unparalleled advantages in the field of sunscreen skincare products due to its low irritation, high biocompatibility, and excellent sun protection effect.

[0025] In a first aspect, the present invention provides a composition with anti-ultraviolet radiation function, wherein, by mass percentage, the main active ingredients include trehalose 0.1%-5%, allantoin 0.01%-1%, betaine 0.1%-5%, panthenol 0.1%-1%, nicotinamide 0.1%-5%, dipotassium glycyrrhizate 0.01%-1%, 4-hydroxyethylpiperazine ethanesulfonic acid 0.1%-5%, 5D-fullerene 0.05%-5%, ergothioneine pine mushroom extract 0.1%-10%, and glycyrrhizin 0.001%-0.1%.

[0026] Preferably, the main active ingredients of the above-mentioned composition with anti-ultraviolet radiation function, based on mass percentage, include trehalose 0.1%-2%, allantoin 0.05%-0.3%, betaine 0.1%-3%, panthenol 0.3%-0.8%, nicotinamide 1%-5%, dipotassium glycyrrhizate 0.1%-0.3%, 4-hydroxyethylpiperazine ethanesulfonic acid 0.1%-0.7%, 5D-fullerene 1%-4%, ergothioneine pine mushroom extract 0.5%-5%, and glycyrrhizin 0.008%-0.3%.

[0027] Secondly, the present invention also provides a fullerene essence, comprising the above-mentioned composition, and further comprising, by weight percentage: 0.01%-0.2% EDTA-2Na, 1%-5% glycerol polyether-26, 0.01%-2% xanthan gum, 0.06%-2% carbomer, 0.5%-10% 1,3-butanediol, 0.1%-0.6% p-hydroxyacetophenone, 0.1%-2% 1,2-hexanediol, 0.1%-2.5% triethanolamine, 0.1%-5% PEG / PPG-17 / 6 copolymer, 0.001%-5% solubilizer, 0.001%-0.5% fragrance, 0.01%-2% β-glucan, with the balance being deionized water.

[0028] It should be noted that the 5D-fullerene in this invention is a composite component, comprising, by mass percentage: 0.001%-0.015% sodium hyaluronate cross-linked polymer, 0.1%-0.3% acetylated sodium hyaluronate, 0.6%-1% sodium hyaluronate, 0.1%-0.2% fullerene, 0.05%-0.6% hydrolyzed sodium hyaluronate, 0.1%-0.2% ethylhexylglycerin, 4%-6% 1,2-pentanediol, with the balance being deionized water.

[0029] Ergothioneine and pine mushroom extract are components that, by mass percentage, include ergothioneine ≥0.013%, pine mushroom extract 94.88%-94.89%, 1,2-pentanediol 5.0%-5.5%, and ethylhexylglycerin 0.08%-0.1%.

[0030] The solubilizer, by weight percentage, comprises 34%-38% PEG-40 hydrogenated castor oil, 51%-57% PPG-26 butanol polyether-26, and 9%-11% water.

[0031] Thirdly, the present invention also provides a method for preparing the above-mentioned fullerene essence, comprising the following steps: Step 1: Weigh each raw material according to the formula, mix the raw materials in groups to obtain the prepared phases A, B, C, D and E; Step 2: Heat, homogenize and / or dissolve the phase obtained in Step 1; Step 3: Mix the phases to obtain fullerene essence.

[0032] In step 1, the preparation of phase A includes the preparation of phases A1 and A2. The preparation of phase A1 includes: accurately weighing xanthan gum, carbomer, and glycerol polyether-26 using an electronic balance, and mixing them evenly in a clean, dry container to obtain phase A1.

[0033] In step 1, the preparation of phase A2 includes: placing deionized water, trehalose, EDTA-2Na, allantoin, and betaine in a clean, dry container and mixing them evenly to obtain phase A2.

[0034] In step 1, the preparation of phase B includes: weighing triethanolamine and placing it in a container to obtain phase B.

[0035] The preparation of phase C includes: weighing 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone, and glycyrrhizin extract, mixing them evenly, and placing them in a container to obtain phase C.

[0036] The preparation of phase D includes: weighing 5D-fullerene, ergothioneine pine mushroom extract, nicotinamide, dipotassium glycyrrhizate, 4-hydroxyethylpiperazine ethanesulfonic acid, panthenol, β-glucan, PEG / PPG-17 / 6 copolymer, and deionized water, mixing them thoroughly, placing them in a container to obtain phase D, and heating until dissolved. The heating temperature is 30-50℃, for example, 30℃, 35℃, 40℃, 45℃, and 50℃.

[0037] The preparation of phase E includes: weighing the fragrance, solubilizer, and water, mixing them evenly, placing them in a container to obtain phase E, and heating until dissolved. The heating temperature is 30-50℃, for example, 30℃, 35℃, 40℃, 45℃, or 50℃.

[0038] Step 2, which involves heating, homogenizing, and / or dissolving the phase obtained in Step 1, includes the following steps: Step 2-1: Mix phase A1 and phase A2 evenly, heat in a water bath until all components are fully dissolved, stir, and homogenize at a certain speed to obtain the treated phase A; Step 2-2: Heat phase B and phase C separately in a water bath until all components are fully dissolved to obtain dissolved phase B and phase C.

[0039] In step 2-1, the water bath temperature is 70-100℃, for example, 70℃, 75℃, 80℃, 85℃, 90℃, 95℃, 100℃. The water bath heating time is 10-50 minutes, for example, 10 minutes, 20 minutes, 30 minutes, 35 minutes, 40 minutes, 45 minutes, 50 minutes.

[0040] In step 2-1, the rotation speed is 2000-10000 rpm, for example, 2000 rpm, 4000 rpm, 5000 rpm, 7000 rpm, 9000 rpm, and 10000 rpm. The homogenization time is 0.5-20 minutes, for example, 0.5 minutes, 1 minute, 2 minutes, 3 minutes, 5 minutes, 8 minutes, 10 minutes, 12 minutes, 15 minutes, and 20 minutes. The purpose of homogenization is to improve the fineness and stability of the product.

[0041] In step 2-2, the water bath temperature is 40-80℃, for example, 40℃, 45℃, 50℃, 55℃, 60℃, 65℃, 70℃, 75℃, 80℃.

[0042] Step 3, mixing the phases, includes the following steps: Step 3-1: Cool the treated phase A, slowly add the dissolved phase B while stirring until it is evenly mixed, then add the dissolved phase C while stirring until it is evenly mixed to obtain a mixed phase; Step 3-2: Cool the mixed phase, add the dissolved D phase while stirring until the mixture is uniform, then add the dissolved E phase while stirring until the mixture is uniform, to obtain a composition with UV protection function.

[0043] In step 3-1, phase A is cooled to 55-65℃, for example, 55℃, 60℃, or 65℃.

[0044] In step 3-2, the mixed phase is cooled to 35-45℃, for example, 35℃, 40℃, 45℃.

[0045] Thirdly, the present invention also provides the application of the above-mentioned composition with anti-ultraviolet radiation function in the preparation of sunscreen essence.

[0046] The present invention will now be described in detail with reference to specific embodiments.

[0047] Examples 1-3 of the present invention provide a composition with ultraviolet radiation protection function and a method for preparing the same. The composition of the compositions of Examples 1-3 and Comparative Examples 1-5 is shown in Table 1. The trade names and models of the raw materials are shown in Table 2.

[0048] Example 1 The preparation of the composition with ultraviolet radiation protection function in this embodiment includes the following steps: Step 1: Accurately weigh xanthan gum, carbomer, and glyceryl polyether-26 using an electronic balance, and mix them thoroughly in a clean, dry container to obtain phase A1; place deionized water, trehalose, EDTA-2Na, allantoin, and betaine in a clean, dry container to obtain phase A2; weigh triethanolamine and place it in a container to obtain phase B; weigh 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone, and glycyrrhizin, mix them thoroughly, and place them in a container to obtain phase C; weigh 5D-fullerene, ergothionein pine mushroom extract, nicotinamide, dipotassium glycyrrhizate, 4-hydroxyethylpiperazine ethanesulfonic acid, panthenol, β-glucan, PEG / PPG-17 / 6 copolymer, and deionized water, mix them thoroughly, and place them in a container to obtain phase D, and heat at 30°C until dissolved; prepare phase E by weighing fragrance, solubilizer, and water, mixing them thoroughly, placing them in a container to obtain phase E, and heating at 30°C until dissolved. Step 2: Mix phases A1 and A2 evenly, heat in a water bath (70℃, 50 minutes) until all components are fully dissolved, and homogenize at a certain speed (3000 rpm) to obtain the treated phase A; heat phases B and C separately in a water bath (40℃) until all components are fully dissolved to obtain the dissolved phases B and C. Step 3: Cool the treated phase A to 55°C, slowly add the dissolved phase B while stirring until the mixture is homogeneous, then slowly add the dissolved phase C while stirring until the mixture is homogeneous to obtain a mixed phase; cool the mixed phase to 35°C, slowly add the dissolved phase D while stirring until the mixture is homogeneous, then slowly add the dissolved phase E while stirring until the mixture is homogeneous to obtain a composition with UV protection function.

[0049] Example 2 The preparation of the composition with ultraviolet radiation protection function in this embodiment includes the following steps: Step 1: Accurately weigh xanthan gum, carbomer, and glyceryl polyether-26 using an electronic balance, and mix them thoroughly in a clean, dry container to obtain phase A1; place deionized water, trehalose, EDTA-2Na, allantoin, and betaine in a clean, dry container to obtain phase A2; weigh triethanolamine and place it in a container to obtain phase B; weigh 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone, and glycyrrhizin, mix them thoroughly, and place them in a container to obtain phase C; weigh 5D-fullerene, ergothionein pine mushroom extract, nicotinamide, dipotassium glycyrrhizate, 4-hydroxyethylpiperazine ethanesulfonic acid, panthenol, β-glucan, PEG / PPG-17 / 6 copolymer, and deionized water, mix them thoroughly, and place them in a container to obtain phase D, and heat at 40°C until dissolved; prepare phase E by weighing fragrance, solubilizer, and water, mixing them thoroughly, placing them in a container to obtain phase E, and heating at 40°C until dissolved. Step 2: Mix phases A1 and A2 evenly, heat in a water bath (85℃, 30 minutes) until all components are fully dissolved, and homogenize at a certain speed (5000 rpm) to obtain the treated phase A; heat phases B and C separately in a water bath (60℃) until all components are fully dissolved to obtain the dissolved phases B and C. Step 3: Cool the treated phase A to 60°C, slowly add the dissolved phase B while stirring until the mixture is homogeneous, then slowly add the dissolved phase C while stirring until the mixture is homogeneous to obtain a mixed phase; cool the mixed phase to 40°C, slowly add the dissolved phase D while stirring until the mixture is homogeneous, then slowly add the dissolved phase E while stirring until the mixture is homogeneous to obtain a composition with UV protection function.

[0050] Example 3 The preparation of the composition with ultraviolet radiation protection function in this embodiment includes the following steps: Step 1: Accurately weigh xanthan gum, carbomer, and glyceryl polyether-26 using an electronic balance, and mix them thoroughly in a clean, dry container to obtain phase A1; place deionized water, trehalose, EDTA-2Na, allantoin, and betaine in a clean, dry container to obtain phase A2; weigh triethanolamine and place it in a container to obtain phase B; weigh 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone, and glycyrrhizin, mix them thoroughly, and place them in a container to obtain phase C; weigh 5D-fullerene, ergothionein pine mushroom extract, nicotinamide, dipotassium glycyrrhizate, 4-hydroxyethylpiperazine ethanesulfonic acid, panthenol, β-glucan, PEG / PPG-17 / 6 copolymer, and deionized water, mix them thoroughly, and place them in a container to obtain phase D, and heat at 50°C until dissolved; prepare phase E by weighing fragrance, solubilizer, and water, mixing them thoroughly, placing them in a container to obtain phase E, and heating at 50°C until dissolved. Step 2: Mix phases A1 and A2 evenly, heat in a water bath (100℃, 10 minutes) until all components are fully dissolved, and homogenize at a certain speed (10000 rpm) to obtain the treated phase A; heat phases B and C separately in a water bath (80℃) until all components are fully dissolved to obtain the dissolved phases B and C. Step 3: Cool the treated phase A to 65°C, slowly add the dissolved phase B while stirring until evenly mixed, then slowly add the dissolved phase C while stirring until evenly mixed to obtain a mixed phase; cool the mixed phase to 45°C, slowly add the dissolved phase D while stirring until evenly mixed, then slowly add the dissolved phase E while stirring until evenly mixed to obtain an essence with anti-ultraviolet radiation function.

[0051] Table 1. Composition of the compositions in the examples and comparative examples (in %)

[0052] Table 2. Trade names and models of raw materials

[0053] The present invention also verified the in vivo safety and UVB protection effect of the prepared essence.

[0054] 1) In vivo safety and efficacy verification of fullerene essence 1. Pre-experiment preparation: Purchase 40 female Balb / c mice, 7-8 weeks old. Perform hair removal on the backs of all mice in all groups. The hair removal area is the entire back (approximately 3cm × 5cm; care should be taken to avoid scratching the back during hair removal). Allow a 2-day acclimatization period after hair removal. First, use a razor to remove longer hairs, then treat the remaining areas with hair removal cream to effectively avoid scratching. Fullerene essence should be prepared in advance as a sample.

[0055] 2. Experimental environment: Ensure that the experimental environment meets the living habits and health needs of mice, including the control of environmental factors such as temperature, humidity, light and noise.

[0056] 3. Experimental Methods: Mice were randomly assigned to two groups of 12 each. One group served as the control group (no treatment), while the other group received the fullerene product (serum). For 14 consecutive days, a thin layer of the product was applied to the back of each mouse, completely covering the skin. Approximately 0.6g was used daily. During the 25-day observation period, the condition of the mice's back skin was photographed and recorded (see...). Figure 1 The mice were recorded for weight and other parameters. Three mice from each group were sacrificed at 3 and 11 days later. Blood was collected from the eyeballs for routine blood tests and blood biochemistry tests, and blood samples from the heart, liver, spleen, lungs, and kidneys were collected for histopathological analysis (H&E staining).

[0057] 4. Experimental Procedures: Perform various experimental procedures on the mice according to the experimental requirements, such as hair removal, application of sunscreen, and ultraviolet irradiation. During these procedures, ensure the comfort and safety of the mice, avoiding unnecessary harm and stress. Ensure adequate food and water supply to maintain the mice's good condition.

[0058] Experimental results are as follows Figure 1 As shown, at each time point, no abnormalities such as erythema, edema, or peeling were observed in the skin of any group of mice; by day 20, healthy and shiny hair had grown on the backs of the mice, and there was no significant difference in the number of hair bulbs in the hair follicles among the groups. This indicates that the essence neither affected hair growth nor triggered an acute allergic reaction in the mice's skin. Furthermore, histological observation of mouse skin... Figure 2 Based on the results, the skin treated with the serum showed no abnormal reactions such as redness, swelling, or structural abnormalities. In conclusion, this fully demonstrates the serum's good safety profile.

[0059] Blood samples were taken from the eyeballs of experimental mice for blood testing. Analysis of routine blood tests and blood biochemical indicators revealed no significant differences in liver and kidney function indicators among the groups. No abnormal changes in liver and kidney function indicators caused by fullerene intervention were observed. Figure 3 , Figure 4This result directly indicates that fullerenes did not produce significant systemic toxicity in mice during the experimental period and had no adverse effects on the function of important metabolic organs (liver and kidneys). Combined with histological observation of H&E staining of internal organs such as the heart, liver, spleen, lungs, and kidneys in mice, it shows that... Figure 5 In the serum group, compared with the control group, no significant pathological abnormalities were observed in the tissue morphology of any organs on days 3, 11, and 20. Meanwhile, weight monitoring results showed (…). Figure 6 The weight gain trend of the experimental animals in the serum group was consistent with that of the control group over time, and no abnormal weight gain was observed due to serum intervention. These observations further indicate that the fullerene serum did not adversely affect the normal growth and development of mice.

[0060] 2) In vivo verification of the UVB protection effect of fullerene essence The selection and treatment of mice in the early stages were consistent with the safety evaluation of the aforementioned materials, and the process began after the mice had passed the adaptation period.

[0061] Experimental grouping: (randomly assigned), 12 animals per group.

[0062] (1) Control group (no treatment) (2) UVB group (irradiated with UVB, without applying sample) (3) UVB + essence group (UVB irradiation, sample application) Experimental methods: Mice were placed 25 cm below the light source. In the first phase of the experimental group, skin tolerance was established through irradiation for 4 days, with a total daily dose of approximately 300 mJ / cm². 2 After a 3-day rest period, the mice underwent a second phase of co-irradiation for 3 days, with the same irradiation intensity for 30 minutes daily. Samples were applied 30 minutes before irradiation, with a single application at a concentration of 2 mg / cm³. 2 After irradiation, the same essence was applied to the backs of the mice daily, and changes in the skin on the backs of the mice were observed and photographed daily. The total experimental period was 20 days. The mice were sacrificed on days 3, 11, and 20, and their skin was collected for histological sectioning and staining analysis.

[0063] Precautions: After applying the sample to the mice before UVB irradiation, the mice in the experimental group need to be separated into different cages to prevent the sample from being licked by the mice in the same cage. After the sample is absorbed, they should be put back into the cage for irradiation.

[0064] like Figure 7As shown, there were significant differences in the condition of the skin on the backs of mice at different time points after UVB radiation treatment: On day 3 after radiation, the skin on the backs of mice in the UVB group showed obvious redness and swelling with a darker color, while the skin on the backs of mice in the UVB+ essence group showed no significant damage and was more moisturized and had a finer texture compared to the control group; on day 11 after radiation, the skin on the backs of mice in the UVB group had ulcerated, while the UVB+ essence group only showed a few small red spots without ulceration, swelling, or other serious damage; on day 20 after radiation, the damaged skin on the backs of mice in the UVB+ essence group showed a trend of scab formation and healing, while the UVB group, although scabs formed, had deeper wounds and a relatively slow healing process. In conclusion, the fullerene essence not only has a good protective effect against skin damage caused by UVB radiation, but also exhibits good moisturizing effects during use and is easily absorbed by the skin.

[0065] Experimental results: Analysis based on H&E staining results of mouse dorsal skin tissue ( Figure 8 Compared to the control group, the UVB+ serum group showed a significant positive effect, specifically in successfully inducing the formation of a new epidermal layer. The UVB group, however, still exhibited edema, epidermal hyperplasia, and relatively severe inflammation. Cell infiltration indicates that the skin wounds in this group of mice have not yet fully healed.

[0066] Experimental results: Observation was performed using Masson staining of the skin on the back of mice ( Figure 9 We can clearly see the distribution of collagen fibers in the dermis. These collagen fibers, stained with aniline blue, appear as blue areas. In the UVB+ serum group, the collagen fibers in the dermis exhibit a continuous and orderly arrangement. In contrast, the inflammatory infiltration areas in the UVB group show a sparse distribution of collagen fibers. This comparative result indicates that fullerenes have a significant protective effect in the prevention and treatment of acute radiation dermatitis.

[0067] 3) In vitro verification of the UVB protection effect of fullerene essence Synergistic test of UV protection efficacy: Test principle: The sample is evenly coated onto a standard UV-transmitting substrate (such as a quartz plate / PMMA plate). The UVB transmittance of the "uncoated substrate" and the "coated substrate" are measured separately. The SPF value is calculated using a formula. The higher the SPF value, the stronger the protection against UVB. The formula is as follows:

[0068] Experimental Methods: 3×3 cm polymethyl methacrylate (PMMA) plates were selected, wiped clean with anhydrous ethanol, and placed in a UV spectrophotometer. Following the cosmetic SPF testing standard application amount (2 mg / cm²), four groups of samples were evenly applied to the surface of four quartz plates using sterile cotton swabs. The application process employed a "cross-hatching method" (first applying horizontally, then vertically for additional application) to avoid air bubbles or missed areas. The coated quartz plates were placed in a 37℃ incubator for 30 minutes, and then placed in a UV spectrophotometer to measure the average transmittance within the same wavelength range (290-320 nm). Each group of samples was tested three times, and the average transmittance was used to calculate the SPF value. The results are shown in Table 3.

[0069] Table 3. Average SPF values ​​of the examples and comparative examples

[0070] Combining ultraviolet transmittance testing and sun protection factor (SPF) analysis, significant differences in ultraviolet protection performance were observed among different samples. For example... Figure 10 As shown, Example 2 exhibits excellent UV blocking capability across the entire 290-400 nm wavelength range, especially in the 290-310 nm range (the critical UVB damage band), where the transmittance rapidly increases from near 0 to over 40%. Combined with the SPF value, this confirms that it possesses extremely strong UVB protection synergy, making it the best performing sample in terms of protection among the tested samples.

[0071] As shown in Table 3, the average SPF value of the essence samples of this invention is 9.39-11.48. However, when only niacinamide (Comparative Example 1) or ergothioneine (Comparative Example 3) is added, the transmittance across the entire wavelength range is close to 100%, with no UV protection capability, and the average SPF values ​​are only 2.63 and 2.61, respectively. Comparative Example 2 only adds 5D fullerene, which has only a very weak protective effect, with an average SPF value of only 3.46. When the amount of 5D fullerene added is too small (Comparative Example 4) or too large (Comparative Example 5), it only shows a very weak UV blocking effect in a specific wavelength range, and the overall protection capability is still at a very weak level, with average SPF values ​​of only 4.72 and 5.37, respectively.

[0072] Furthermore, the data in Table 3 also shows that the average SPF value of each embodiment of the present invention is greater than the sum of the average SPF values ​​of comparative examples 1-3. This proves that the present invention significantly improves the ultraviolet protection capability of the product by optimizing the components and content and achieving synergistic effects of multiple components, achieving a 1+1>2 effect. It is significantly superior to each comparative example in terms of sun protection performance, providing a strong reference for the research and development of high-performance sun protection materials.

[0073] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention.

Claims

1. A composition with ultraviolet radiation protection function, characterized in that, Based on mass percentage, the main active ingredients include trehalose 0.1%-5%, allantoin 0.01%-1%, betaine 0.1%-5%, panthenol 0.1%-1%, nicotinamide 0.1%-5%, dipotassium glycyrrhizate 0.01%-1%, 4-hydroxyethylpiperazine ethanesulfonic acid 0.1%-5%, 5D-fullerene 0.05%-5%, ergothioneine pine mushroom extract 0.1%-10%, and glycyrrhizin 0.001%-0.1%.

2. The composition according to claim 1, characterized in that, Based on mass percentage, the main active ingredients include trehalose 0.1%-2%, allantoin 0.05%-0.3%, betaine 0.1%-3%, panthenol 0.3%-0.8%, nicotinamide 1%-5%, dipotassium glycyrrhizate 0.1%-0.3%, 4-hydroxyethylpiperazine ethanesulfonic acid 0.1%-0.7%, 5D-fullerene 1%-4%, ergothioneine pine mushroom extract 0.5%-5%, and glycyrrhizin 0.008%-0.3%.

3. A fullerene essence, characterized in that, The composition comprising, by weight percentage, of the following: 0.01%-0.2% EDTA-2Na, 1%-5% glycerol polyether-26, 0.01%-2% xanthan gum, 0.06%-2% carbomer, 0.5%-10% 1,3-butanediol, 0.1%-0.6% p-hydroxyacetophenone, 0.1%-2% 1,2-hexanediol, 0.1%-2.5% triethanolamine, 0.1%-5% PEG / PPG-17 / 6 copolymer, 0.001%-5% solubilizer, 0.001%-0.5% fragrance, 0.01%-2% β-glucan, with the balance being deionized water.

4. The fullerene essence according to claim 3, characterized in that, Its average SPF value is 9.39-11.

48.

5. A method for preparing a fullerene essence, characterized in that, The preparation of the fullerene essence according to claim 3 or 4 comprises the following steps: Step 1: Weigh each raw material according to the formula, mix the raw materials in groups to obtain the prepared phases A, B, C, D and E; Step 2: Heat, homogenize and / or dissolve the phase obtained in Step 1; Step 3: Mix the phases to obtain fullerene essence.

6. The preparation method according to claim 5, characterized in that, In step 1, preparing phase A includes preparing phases A1 and A2; The preparation of phase A1 includes: weighing xanthan gum, carbomer, and glycerol polyether-26, mixing them to obtain phase A1; The preparation of phase A2 involves mixing deionized water, trehalose, EDTA-2Na, allantoin, and betaine to obtain phase A2.

7. The preparation method according to claim 5, characterized in that, In step 1, the preparation of phase C includes: weighing 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone, and glycyrrhizin, mixing them, and obtaining phase C.

8. The preparation method according to any one of claims 5-7, characterized in that, The preparation of phase D includes: weighing 5D-fullerene, ergothioneine pine mushroom extract, nicotinamide, dipotassium glycyrrhizate, 4-hydroxyethylpiperazine ethanesulfonic acid, panthenol, β-glucan, PEG / PPG-17 / 6 copolymer, and deionized water, and mixing them to obtain phase D.

9. The preparation method according to claim 8, characterized in that, Step 2, which involves heating, homogenizing, and / or dissolving the phase obtained in Step 1, includes the following steps: Step 2-1: Mix phase A1 and phase A2, heat in a water bath until the components dissolve, stir, and obtain the treated phase A; Step 2-2: Heat phase B and phase C separately in a water bath until the components dissolve, to obtain dissolved phase B and phase C.

10. The use of the composition according to claim 1 or 2 in the preparation of a UV-protective serum.

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