A moisturizing and soothing plant composition, and a preparation method and application thereof

Abstract

The present application relates to the field of cosmetics, in particular to a moisturizing and soothing plant composition, and a preparation method and application thereof. The present application develops a moisturizing and soothing plant composition by compounding Lycium barbarum extract, Paeonia lactiflora root extract, Sophora flavescens root extract, Jingnan Chrysanthemum extract and Rhodiola rosea extract. The moisturizing and soothing plant composition combines the "principle of monarch, minister and assistant" in traditional Chinese medicine theory, uses Paeonia lactiflora as monarch medicine, Sophora flavescens root as minister medicine, Jingnan Chrysanthemum extract as assistant medicine, and Lycium barbarum and Rhodiola rosea as minister medicine, which can synergize and combine with each other to strengthen the efficacy of plants in soothing and moisturizing. The moisturizing and soothing plant composition prepared by the present application has high safety and remarkable soothing and moisturizing effects when applied to cosmetics.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, and more particularly to a moisturizing and soothing plant composition, its preparation method, and its application. Background Technology

[0002] The skin is divided into the epidermis and dermis. The epidermal structure, from the outside in, consists of the stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum, and stratum basale. The skin barrier can be divided into three layers in terms of cells and structure: the air-liquid interface barrier, the fluid-liquid interface barrier, and the immune barrier. The air-liquid interface barrier is the stratum corneum, which is the main executor of the skin's physical barrier function. The stratum corneum is composed of flattened, anucleate keratinocytes stacked on top of each other. The spaces between the keratinocytes in each layer are filled with a lipid matrix, composed of FLG (keratinized intermediate filament polymer), water-soluble lipids, proteins (enzymes), and natural moisturizing factor (NMF). FLG is an important protein for maintaining the integrity of the epidermal barrier function, and NMF is an important molecule affecting skin hydration. A reduction or absence of NMF weakens the skin barrier function, leading to various skin diseases.

[0003] Normal skin can develop various skin problems due to a variety of internal and external factors. External factors include climate conditions; low temperatures cause blood vessels in the skin to constrict, reducing the secretion of sebaceous and sweat glands, leading to dry skin, flaking, and itching. For example, in winter, low temperatures often cause varying degrees of itching and flaking on the lower legs. Secondly, low air humidity causes excessive moisture loss from the skin, reducing the water content of the stratum corneum, impairing the skin barrier function, and making it prone to cracking. In high temperatures, blood vessels in the skin dilate, exacerbating inflammatory reactions; sensitive skin may experience redness and burning sensations in high temperatures. Environmental pollution, with pollutants in the air (such as dust, heavy metals, and chemicals) adhering to the skin surface, can cause varying degrees of allergic reactions with prolonged exposure, such as intense itching and persistent redness. Improper use of cosmetics or skincare products, or excessive cleansing, can thin the skin and damage the stratum corneum, leading to dryness, sensitivity, and increased susceptibility to skin inflammation.

[0004] Currently, common moisturizers in skincare products include glycerin, butylene glycol, propylene glycol, and polyethylene glycol. Some moisturizers, such as glycerin, propylene glycol, and polyethylene glycol, may cause a sticky feeling when used at high concentrations. Propylene glycol and PEG can irritate the skin and may cause sensitivity. In low-humidity environments, these moisturizers may draw moisture from deeper layers of the skin, leading to dryness. To facilitate use by engineers, many cosmetic ingredients on the market are primarily water-soluble or alcohol-soluble, but these are difficult to integrate into oil-based or oil-soluble products such as essential oils and makeup removers. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the existing technology and provide a moisturizing and soothing plant composition, its preparation method and application.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a moisturizing and soothing plant composition comprising the following components in parts by weight: 1-10 parts of Ningxia wolfberry extract, 1-5 parts of peony root extract, 0.5-7.5 parts of sophora root extract, 0.5-5 parts of Shennong chrysanthemum extract, and 0.05-0.5 parts of rhodiola rosea extract.

[0007] To address the issue of irritation from some skincare product ingredients, this invention develops a moisturizing and soothing plant-based composition by compounding extracts of Ningxia wolfberry, peony root, sophora root, chrysanthemum, and rhodiola. Among the ingredients, Ningxia wolfberry is the only wolfberry variety listed in the Chinese Pharmacopoeia, rich in plant polysaccharides, proteins, vitamins, and other nutrients. Studies have shown that it plays an important role in anti-tumor activity, lipid regulation, and blood sugar reduction. Peony root contains polysaccharides, paeoniflorin, tannins, flavonoids, and is rich in volatile components, giving it various pharmacological effects such as antibacterial, neuroprotective, anti-tumor, anti-inflammatory, antithrombotic, and antioxidant properties. Sophora flavescens contains alkaloids, flavonoids, and saponins. Modern pharmacological studies have shown that it has certain anti-inflammatory and antibacterial effects. The Shennong Xiangju extract in this combination uses Shennong Xiangju, a variety of the Chrysanthemum genus in the Shennongjia region, as its main chemical components. Shennong Xiangju has flavonoids and terpenoids, exhibiting good free radical scavenging ability and tissue protection. Rhodiola rosea is a perennial herbaceous plant of the Crassulaceae family and a precious natural medicinal material. It is rich in flavonoids and polyphenols, with rhodioloside being a key component. Rhodiola rosea has certain antibacterial and anti-inflammatory effects, can scavenge free radicals, resist ultraviolet radiation, and delay aging. This invention combines the above plant extracts within a specific range. Multiple experiments have demonstrated that the resulting composition is safe and also possesses good moisturizing and soothing effects.

[0008] Specifically, ordinary water extraction methods can be used to extract Ningxia wolfberry, Shennong chrysanthemum, and Rhodiola rosea to obtain Ningxia wolfberry extract, Shennong chrysanthemum extract, and Rhodiola rosea extract, respectively; ordinary alcohol extraction methods can be used to extract peony root and sophora flavescens root to obtain peony root extract and sophora flavescens root extract, respectively. In the peony root extraction process, ultrasound-assisted extraction is used to target and enrich its active ingredients; in the sophora flavescens root extraction process, the pH is appropriately adjusted to acidic conditions to convert it into salts, increasing its solubility. Extensive research has shown that the extraction methods of the above-mentioned plant extracts do not affect the efficacy of the plant composition of this invention.

[0009] Preferably, the moisturizing and soothing plant composition comprises the following components in parts by weight: 5-10 parts of Ningxia wolfberry extract, 3.5-5 parts of peony root extract, 7-7.5 parts of sophora root extract, 1.5-5 parts of Shennong chrysanthemum extract, and 0.2-0.5 parts of rhodiola rosea extract.

[0010] Preferably, the mass ratio of the peony root extract to the sophora root extract is peony root extract: sophora root extract = 1: (1.5-2.5).

[0011] Through experimental research, this invention has discovered that, in accordance with the principle of "principal, assistant, adjuvant, and guide" in traditional Chinese medicine, using peony as the principal herb, sophora flavescens root as the assistant herb, chrysanthemum extract as the adjuvant herb, and wolfberry and rhodiola rosea as the guide herb, the above plant extracts are compounded in a specific mass ratio to obtain a moisturizing and soothing plant composition. The synergistic and combined effects of these ingredients can enhance their soothing and moisturizing efficacy.

[0012] Preferably, the moisturizing and soothing plant composition further includes 20-25 parts butylene glycol, 12-15 parts glycerin, 0.2-0.4 parts p-hydroxyacetophenone, 0.5-1 parts 1,2-hexanediol, and water to a total of 100 parts.

[0013] Preferably, the preparation method of the moisturizing and soothing plant composition includes the following steps: The extracts of Ningxia wolfberry, peony root extract, sophora root extract, chrysanthemum extract, rhodiola rosea extract, butylene glycol, glycerin, p-hydroxyacetophenone, 1,2-hexanediol, and water were stirred and mixed evenly to obtain the moisturizing and soothing plant composition.

[0014] Secondly, the present invention provides the application of the aforementioned moisturizing and soothing plant composition in the preparation of moisturizing and soothing cosmetics.

[0015] Preferably, the dosage form of the cosmetic includes water, lotion, and cream.

[0016] Thirdly, the present invention provides a moisturizing and soothing cosmetic, comprising the aforementioned moisturizing and soothing plant composition and excipients.

[0017] Preferably, the excipients include at least one of a humectant, a thickener, a chelating agent, a preservative, and water.

[0018] Preferably, the moisturizing and soothing cosmetic comprises the following components by weight percentage: 1-38.5% moisturizing and soothing plant composition, 1.1-36% humectant, 0.01-0.05% thickener, 0.01-1% chelating agent, 0.1-1% preservative, and the balance being deionized water.

[0019] Preferably, the moisturizer includes at least one of panthenol, betaine, erythritol, sodium hyaluronate, butylene glycol, glycerin, propylene glycol, polyglycerol-10, and dipropylene glycol.

[0020] Preferably, the thickener includes at least one of xanthan gum, hydroxyethyl cellulose, and β-glucan.

[0021] Preferably, the chelating agent comprises disodium EDTA.

[0022] Preferably, the preservative includes at least one selected from p-hydroxyacetophenone, methyl propylene glycol, capryloyl hydroxamic acid, glyceryl caprylate, 1,2-hexanediol, phenoxyethanol, and ethylhexylglycerin.

[0023] The beneficial effects of this invention are as follows: In the moisturizing and soothing plant composition of the present invention, wolfberry, peony, and sophora root are common plant resources in China, which are natural, safe, and have a pleasant fragrance. As skin care ingredients, they have the characteristics of high safety.

[0024] The moisturizing and soothing plant composition of the present invention combines the "principal, assistant, adjuvant, and guide" principle in traditional Chinese medicine theory, using peony as the principal herb, sophora flavescens root as the assistant herb, chrysanthemum extract as the adjuvant herb, and wolfberry and rhodiola rosea as the guide herb, so that they work together to exert a soothing and moisturizing effect.

[0025] This invention uses a moisturizing and soothing plant composition in cosmetics to prepare a moisturizing and soothing spray. This product is easy to carry and use, and can relieve symptoms such as dryness or itching of the skin. Attached Figure Description

[0026] Figure 1 The results of the allantoic membrane vascular experiment on chicken embryos in 0.9% saline solution.

[0027] Figure 2 The results of the allantoic membrane vascular experiment in chicken embryos with 0.05% K12 are shown in the figure.

[0028] Figure 3 The image shows the results of the chicken embryo allantoic membrane vascular test of the moisturizing and soothing plant composition in Example 2.

[0029] Figure 4 The image shows the results of the chicken embryo allantoic membrane vascular test of the moisturizing and soothing plant composition in Comparative Example 2.

[0030] Figure 5 Figure 7 shows the results of the chicken embryo allantoic membrane vascular test of the moisturizing and soothing plant composition used in Comparative Example 7.

[0031] Figure 6 The changes in the face of volunteers after using the moisturizing and soothing spray of Application Example 1.

[0032] Figure 7 The changes in the faces of volunteers after using the moisturizing and soothing spray in Comparative Example 1. Detailed Implementation

[0033] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0034] The extracts of Ningxia wolfberry, peony root, sophora root, chrysanthemum and rhodiola used in this invention are all from Natural (Guangzhou) New Materials Research and Development Co., Ltd.

[0035] Examples 1-7: Examples of the moisturizing and soothing plant compositions of the present invention; the components and formulations of the moisturizing and soothing plant compositions described in Examples 1-7 are shown in Table 1.

[0036] Table 1 The preparation method of the moisturizing and soothing plant composition includes the following steps: (1) Mix Ningxia wolfberry extract, peony root extract, sophora root extract, chrysanthemum extract, rhodiola rosea extract, water, butylene glycol and glycerin evenly, and heat at 80-85℃ until completely dissolved to obtain a mixture; (2) When the temperature drops to 40-45℃, add p-hydroxyacetophenone and 1,2-hexanediol to the mixture obtained in step (1), stir evenly, and then cool down to 35-38℃ to obtain the moisturizing and soothing plant composition.

[0037] Comparative Examples 1-7: Comparative examples of the moisturizing and soothing plant compositions of the present invention; the components and formulations of the moisturizing and soothing plant compositions of Comparative Examples 1-7 are shown in Table 2.

[0038] Table 2 The only difference between Comparative Example 1 and Example 3 is that the Shennong Xiangju extract in Comparative Example 1 was replaced with an equal amount of purslane extract, while the rest was the same as in Example 3.

[0039] The only difference between Comparative Examples 3-7 and Example 3 is that: Comparative Example 3 does not contain Ningxia wolfberry extract; Comparative Example 4 does not contain peony root extract; Comparative Example 5 does not contain sophora root extract; Comparative Example 6 does not contain Shennong chrysanthemum extract; Comparative Example 7 does not contain rhodiola rosea extract; and missing components are made up with an equal part by weight of water.

[0040] The preparation method of the moisturizing and soothing plant composition is the same as in the examples.

[0041] Application Example 1: An application embodiment of the moisturizing and soothing cosmetic of the present invention; specifically, the moisturizing and soothing cosmetic is a moisturizing and soothing spray.

[0042] The formulation of the moisturizing and soothing spray described in Application Example 1 is shown in Table 3.

[0043] Table 3: Moisturizing and Soothing Spray Formula The preparation method of the moisturizing and soothing spray includes the following steps: (1) Mix water, butanediol, p-hydroxyacetophenone, EDTA-2Na, xanthan gum and sodium hyaluronate, heat to 65-70℃, and stir until homogeneous to obtain mixture A; (2) When mixture A is cooled to 55-60℃, add 1,2-hexanediol, methylpropanediol, capryloyl hydroxamic acid and glyceryl caprylate and mix evenly. Then add the moisturizing and soothing plant composition and continue stirring and cooling to obtain mixture B. (3) When the mixture B cools down to below 45°C, take a sample for testing. After the physicochemical indicators are qualified, filter the material with a sterilized 200-mesh clean filter cloth; introduce the material into a spray bottle through a sterile pipe, seal it and label it; and obtain the soothing and moisturizing spray.

[0044] Application Comparative Example 1: Comparative examples of the application of the moisturizing and soothing cosmetic of the present invention; the moisturizing and soothing cosmetic is specifically a moisturizing and soothing spray.

[0045] The only difference between the formulation of Comparative Example 1 and Application Example 1 is that 5% of the moisturizing and soothing plant composition of Example 3 in Application Example 1 is replaced with 5% of the moisturizing and soothing plant composition of Comparative Example 5, and the remaining components are the same as those in Application Example 1.

[0046] The preparation method of the moisturizing and soothing spray described in Comparative Example 1 is the same as that in Application Example 1.

[0047] Experiment 1: Soothing Effect Test - Hyaluronidase Inhibition Test The soothing effect was assessed using a hyaluronidase activity inhibition assay. The principle is as follows: Hyaluronidase is an enzyme that degrades hyaluronic acid (HA). Hyaluronic acid is an important component of the extracellular matrix and plays a role in regulating the viscosity and permeability of the intercellular matrix. During inflammation, inflammatory cells (such as macrophages and neutrophils) are activated, releasing various inflammatory factors and enzymes, including hyaluronidase. Increased hyaluronidase activity leads to the degradation of hyaluronic acid, producing low molecular weight hyaluronic acid fragments, which have pro-inflammatory effects. Inhibiting hyaluronidase helps reduce the inflammatory response. A higher hyaluronidase activity inhibition rate reflects a stronger anti-allergic effect, and vice versa. Therefore, inhibition of hyaluronidase activity can serve as an indicator of the anti-inflammatory and anti-allergic response of a sample.

[0048] Test samples: Moisturizing and soothing plant compositions prepared in Examples 1-7 and Comparative Examples 1-7.

[0049] The testing method is as follows: Take 0.5 mL of sample solution and 0.5 mL of hyaluronidase solution, and incubate at 37℃ for 20 min; add 0.1 mL of 2.5 mol / L CaCl2, and incubate at 37℃ for 20 min; add 0.5 mL of sodium hyaluronate solution and incubate at 37℃ for 40 min; place at room temperature for 10 min, add 0.5 mL of distilled water, 0.1 mL of 5 mol / L NaOH solution, and 0.5 mL of acetylacetone solution, and heat in a boiling water bath for 15 min, then immediately cool with ice water for 10 min, place at room temperature for 10 min, add 1.0 mL of LEhrlich reagent, and dilute with 4.3 mL of anhydrous ethanol, place at room temperature for 30 min for color development, and measure the absorbance at 530 nm. Calculate the hyaluronidase inhibition rate according to formula (1): Hyaluronidase inhibition rate (%) = [(AB)-(CD)] / (AB)*100% — Equation (1) In the above formula: A is the absorbance of the control solution (using acetate buffer instead of the sample solution); B represents the absorbance of the control blank solution (using acetate buffer instead of the sample solution and enzyme solution). C represents the absorbance of the sample solution; D is the absorbance of the blank sample solution (using acetate buffer instead of enzyme solution).

[0050] Table 4: Hyaluronidase Inhibition Effect The results are shown in Table 4. The extracts of Ningxia wolfberry, peony root, sophora root, chrysanthemum, and rhodiola rosea in the moisturizing and soothing plant compositions prepared in Examples 1-7 of this invention work together to have a good inhibitory effect on hyaluronidase, with a significant inhibition rate of over 30%, and have a certain soothing effect on symptoms such as itching and discomfort of the skin.

[0051] Overactivation of hyaluronidase can lead to excessive degradation of hyaluronic acid into low molecular weight fragments, affecting the skin's moisturizing ability and skin barrier function, thereby exacerbating inflammation. The moisturizing and soothing plant composition prepared in this embodiment of the invention can inhibit the overactivation of hyaluronidase, reduce the metabolism of hyaluronic acid, promote the repair of damaged components and reduce moisture loss, thereby alleviating the inflammatory response.

[0052] Compared with Examples 5, 6, and 7, Example 2, with its specific mass ratio of peony root extract and sophora root extract, showed superior effects and better synergistic effects. This is because the moisturizing and soothing plant composition of Example 2 uses peony as the principal ingredient and sophora root as the secondary ingredient. Paeoniflorin in peony root binds to the enzyme's active site, blocking its degradation of hyaluronic acid (HA), allowing HA to be retained in the skin and thus enhancing the moisturizing effect. Matrine in sophora root inhibits the NF-κB signaling pathway, reducing the expression of inflammatory factors (such as IL-1β and TNF-α), which are key mediators in inducing hyaluronidase activity. Polyphenols in peony root (such as oxidized paeoniflorin) and flavonoids in sophora root (such as sophora flavonoids) work together to scavenge free radicals and reduce oxidative stress damage to blood vessels. When peony root extract and sophora root extract are combined in a specific ratio, the water solubility of paeoniflorin and the lipid solubility of matrine are optimally balanced, which can promote the penetration and action of both in the skin. If the ratio is exceeded, it will affect the binding target and reduce the synergistic effect.

[0053] The moisturizing and soothing plant composition of this invention is not a simple combination of plant extracts. In the plant extract raw materials used in this invention, paeoniflorin (the principal ingredient) and matrine (the assistant ingredient) inhibit hyaluronidase at different sites, while chrysanthemum volatile oil (the adjuvant ingredient) promotes penetration, resulting in an inhibition rate far exceeding that of single-drug superposition. Peony (NF-κB pathway) + matrine (histamine release) + chrysanthemum (COX-2 / 5-LOX) block the inflammatory cascade reaction at multiple targets, forming a "three-dimensional anti-inflammatory" effect. Lycium barbarum polysaccharide (the adjuvant ingredient) enhances hydration, and rhodioloside (the adjuvant ingredient) improves blood flow. Together with the enzyme-inhibiting effects of the principal and assistant ingredients, they construct a three-dimensional moisturizing system that "reduces loss + promotes absorption + repairs the skin barrier." Compared with Comparative Examples 1-2, in Example 3, the proportions of Ningxia wolfberry extract, peony root extract, sophora root extract, chrysanthemum extract, and rhodiola rosea extract in the moisturizing and soothing plant compositions prepared in Comparative Examples 1-2 exceeded the scope of this invention, or any plant extract was replaced with a similar plant extract, resulting in a significant decrease in the hyaluronidase inhibitory effect. This indicates that the five specific plant extracts need to be combined in a certain proportion to meet the principle of principal, assistant, adjuvant, and guide to achieve the best effect of "harmony" and "balance".

[0054] Traditional Chinese medicine theory emphasizes the importance of "harmony" and "balance." The principal, assistant, adjuvant, and guide herbs are all indispensable. Although the adjuvant and guide herbs are used in small quantities, they harmonize the properties of the herbs and assist the principal and assistant herbs in exerting their effects. Peony root, as the principal herb, is responsible for the core effect of "anti-inflammatory and soothing." Sophora flavescens root, as the assistant herb, assists the principal herb in enhancing its anti-inflammatory and antibacterial effects. Matrine can inhibit histamine release and reduce capillary permeability, forming a synergistic effect with paeoniflorin. The mass ratio of the principal and assistant herbs conforms to the optimal synergistic range of "mutual reinforcement." Chrysanthemum morifolium, as the adjuvant herb, has both "assisting" and "regulating" functions. Its flavonoids and volatile oils can disperse stagnant heat, promote transdermal absorption, and alleviate the bitter and cold nature of Sophora flavescens, enhancing its moisturizing effect. Extracts of Ningxia wolfberry and Rhodiola rosea, as guide herbs, on the one hand, guide the other herbs to the skin target points, and on the other hand, stabilize the overall medicinal properties of the compound through the "yin-yang harmonization" of polysaccharide-glycoside components. Although added in low amounts, Rhodiola rosea extract contains rhodioloside and tyrosol, which, in Traditional Chinese Medicine, are considered "qi-tonifying and blood-activating" herbs that can improve local microcirculation and promote the penetration and absorption of other components. Its complementary polarity (lipid-soluble rhodioloside + water-soluble Lycium barbarum polysaccharides) and harmonizing medicinal properties are irreplaceable. The absence of any one of these plant extracts disrupts the overall balance of the formula, leading to decreased transdermal absorption and metabolic efficiency. As stated in the *Shennong Bencao Jing* (Shennong's Classic of Materia Medica), "Medicines have principal, assistant, adjuvant, and guide herbs to mutually promote and regulate their effects." The efficacy of a compound formula is the combined result of "chemical synergy of components" and "ecological balance of medicinal properties." The absence of any one plant extract will disrupt this dynamic balance, leading to a diminished effect that has a ripple effect throughout the entire formula. Therefore, compared with Comparative Examples 3-7, the moisturizing and soothing plant compositions prepared in Comparative Examples 3-7 lacked any of the following components: Ningxia wolfberry extract, peony root extract, sophora root extract, chrysanthemum extract, and rhodiola rosea extract. This caused an imbalance in the composition, which significantly affected its soothing effect. The hyaluronidase inhibition rate was significantly lower than that in Example 3.

[0055] Experimental Example 2: Soothing Effect Test - Chicken Embryo Allantoic Membrane Vascular Test The chicken embryo chorioallantoic membrane (CAM) test is an early and widely used in vitro method for assessing eye irritation. The chorioallantoic membrane (CAM) is a respiratory membrane surrounding the chicken embryo. Utilizing the intact, clear, and transparent vascular system of the mid-stage CAM in hatched chicken embryos, a certain amount of the test substance is directly contacted with the CAM. After a period of time, changes in CAM toxicity indicators (such as hemorrhage, coagulation, and vascularization) are observed. These indicators reflect changes in the morphology, color, and permeability of blood vessels and vascular networks, as well as phenomena such as CAM protein denaturation and the degree of damage. These indicators are then combined to obtain a score used to assess the eye irritation of the test substance. In this experiment, K12 (sodium lauryl sulfate) was used as an irritant to co-treat the CAM with the test sample. Changes in toxicity indicators were observed to obtain an irritation score, which was then used to evaluate the soothing efficacy of the sample.

[0056] Test samples: The moisturizing and soothing plant compositions prepared in Examples 1-7 and Comparative Examples 1-7 were prepared with physiological saline to a concentration of 3% for testing.

[0057] The testing method is as follows: (1) Preparation of CAM: CAM is prepared using chicken embryos incubated for 9 days; (2) Establishment of K12 stimulation model: 0.3 mL of SDS solution with a mass concentration of 0.05% was added to the surface of chicken embryo chorioallantoic membrane (CAM). The time when bleeding, vascular lysis and coagulation of CAM began to appear within 5 min was recorded. During this period, continuous observation and photography were taken. Each test sample was tested in 6 parallel experiments.

[0058] (3) Test on the soothing effect of raw materials against SDS stimulation: Sample preparation: Each group of samples was prepared at a concentration of 3% for testing, with a K12 concentration of 0.05%. The soothing and irritation-relieving efficacy of each ingredient was tested within the safe concentration range for each ingredient. SDS solution was mixed with different concentrations of soothing ingredients to prepare test samples. 0.3 mL of the solution was dropped onto the CAM surface, and the time it took for bleeding, vascular dissolution, and clotting to begin within 5 minutes was recorded. Continuous observation and photography were conducted throughout the process. Each test solution was tested in six parallel trials.

[0059] (4) Observation of results Bleeding: refers to the outflow of blood from the blood vessels or capillaries of the CAM. Bleeding can manifest in various forms, such as cauliflower-like, smooth, diffuse gauze-like, or punctate bleeding.

[0060] Coagulation: refers to the denaturation of proteins inside and outside blood vessels, usually only seen in large and medium-sized blood vessels, excluding changes in capillaries.

[0061] Vascular dissolution: refers to the disappearance of blood vessels on the CAM membrane, which may be caused by changes in multiple factors such as hemorrhage and changes in vascular wall tension.

[0062] (5) Scoring criteria Experiments conducted using the reaction time method were performed, and stimulus scores were calculated according to equation (2): IS = (301 - t) secH )×5 / 300+(301-t secL )×7 / 300+(301-t secC )×9 / 300——Equation (2) In the above formula: t secH The mean time, in seconds, was observed on the CAM to begin bleeding. t secL The mean time, in seconds, was observed on CAM to initiate vascular dissolution. t secC The mean time, in seconds, is the time observed on CAM for the onset of clotting.

[0063] Stimulation rating results: IS < 1 indicates no irritation; 1 ≤ IS < 5 indicates mild irritation; 5 ≤ IS < 10 indicates moderate irritation; IS ≥ 10 indicates strong irritation / corrosiveness.

[0064] Table 5: Results of Chicken Embryo Allantoic Membrane Vascular Test The results are shown in Table 5 and Figure 1-5 As shown. In the control group, the irritant effect of 0.9% saline solution showed no significant change. Figure 1 The irritation level of 0.05% K12 is rated as moderate. Figure 2 ).

[0065] The Ningxia wolfberry extract, peony root extract, sophora root extract, chrysanthemum extract, and rhodiola rosea extract in the moisturizing and soothing plant compositions prepared in Examples 1-7 of this invention work synergistically to significantly reduce the IS score from >5 to <5. Figure 3 The IS score ranged from 0.9 to 3.52, indicating that the stimulation of the blood vessels in the allantoic membrane of the chicken embryo was significantly reduced to mild irritation.

[0066] Compared with Comparative Examples 1-7, in the moisturizing and soothing plant compositions prepared in Comparative Examples 1-2, the proportions of Ningxia wolfberry extract, peony root extract, sophora root extract, chrysanthemum extract, and rhodiola rosea extract exceeded the scope of this invention, or any plant extract was replaced with a similar plant extract. Consequently, the components could not achieve a good synergistic effect, and the IS scores were all greater than 4 ( ). Figure 4 (This is an increase compared to the previous embodiment.)

[0067] When any of the following components were missing from the moisturizing and soothing plant compositions prepared in Comparative Examples 3-7—including Ningxia wolfberry extract, peony root extract, sophora root extract, chrysanthemum extract, and rhodiola rosea extract—the composition was unbalanced, affecting its soothing effect. Figure 5 From the IS score, all scores are greater than those of the examples.

[0068] In summary, when the skin is stimulated by external factors or surfactants, the moisturizing and soothing plant composition prepared in this invention can have a certain soothing effect on the irritation caused by surfactants.

[0069] Experiment Example 3: Human Body Moisturizing and Soothing Efficacy Test (Histamine) Sensitive skin (SS) specifically refers to a hyperresponsive state of the skin under physiological or pathological conditions. Clinically, it manifests as subjective symptoms such as burning, stinging, itching, and tightness when stimulated by physical, chemical, or psychological factors, with or without objective signs such as erythema, scaling, and telangiectasia. Current research suggests that the development of sensitive skin is a complex process involving the skin barrier, neurovascular system, and immune inflammation. Under the interaction of internal and external factors, the skin barrier function is impaired, leading to enhanced sensory nerve afferent signals, resulting in increased skin reactivity to external stimuli and triggering an immune response. Currently, the commonly used human clinical trial methods for evaluating the efficacy of soothing skincare products include: skin patch testing, lactic acid stinging test, or histamine stimulation test.

[0070] This experiment used a histamine stimulation test to test the soothing and antipruritic effects of the moisturizing and soothing plant compositions of Examples 3 and 4 and Comparative Examples 2, 3 and 5.

[0071] Number of participants and selection criteria: Healthy volunteers were randomly assigned to each test group of 5 people.

[0072] Methods and Procedures: For 2-3 days prior to testing, no products (cosmetics or topical medications) should be used on the test site, and the site should not come into contact with water for 1-3 hours. Three 2 cm × 2 cm areas of skin were randomly marked on the inner forearm of the subject as test areas, designated as a control group and four experimental groups, with at least 1.5 cm between each group. Photographs of the inner forearm of the subject were taken as baseline values ​​before modeling. The initial values ​​of the marked areas were then measured using three probes: the Antsci Skin-SP moisture probe, the Transdermal Water Loss (TEWL) probe, and the Skin-Colorimeter CL400 water skin color test probe. Each area was tested three times, and the average value was taken as the final test value. A 2 cm × 2 cm piece of non-woven fabric was soaked in a 20 mg / mL histamine solution (2% histamine solution). After complete saturation, it was applied to the three areas and left for 3-5 minutes before removal. Subsequent testing began after the surface was dry and free of solution residue. No treatment was performed on the control group. All skin parameters in each area were reassessed as baseline values ​​after modeling. If no reaction is observed in volunteers after 5 minutes of application, the application time can be appropriately increased. Deionized water was applied to the control group, while the test samples (the moisturizing and soothing plant compositions of Examples 3, 4, and Comparative Examples 2, 3, and 5 were all diluted with deionized water to a 5% aqueous solution) were used in the test areas of the experimental groups. The sample usage was 2 mg / cm³. 2 Three probe tests were performed on the subjects 15 minutes after histamine stimulation (before sample use), and 10, 30, and 60 minutes after sample use, with corresponding observations and photographs taken. The volunteers' redness reduction and itch relief were monitored at any time.

[0073] Table 6: Results of Human Efficacy Evaluation Table 7 The results are shown in Tables 6 and 7. Comparing the results of Examples 3 and 4 with those of Comparative Examples 2, 3, and 5, after skin stimulation with histamine, the skin moisture content increased slightly and transepidermal water loss increased significantly. However, after using the moisturizing and soothing plant composition samples of Examples 3 and 4, the moisture content increased significantly after 10 min, 30 min, and 60 min, the transepidermal water loss decreased, and the skin erythema was alleviated.

[0074] In the moisturizing and soothing plant compositions of Comparative Examples 2, 3, and 5, the presence of extracts of Ningxia wolfberry, peony root, sophora root, chrysanthemum, and rhodiola rosea, exceeding the limits of this invention, or lacking any of these components, resulted in an increase in skin moisture levels within 10 minutes. However, after 30 and 60 minutes, skin moisture essentially returned to its original value. Furthermore, the samples in Comparative Examples 2, 3, and 5 did not show significant improvement in transdermal water loss or skin erythema. Therefore, the extracts of Ningxia wolfberry, peony root, sophora root, chrysanthemum, and rhodiola rosea only possess the ability to reduce moisture loss and promote the repair of damaged tissues when used in combination within the component ratios defined in this invention. The plant extracts of this invention synergistically enhance the soothing and moisturizing effects.

[0075] Experiment Example 4: Human Body Moisturizing and Soothing Efficacy Test Test samples: Moisturizing and soothing sprays prepared using Example 1 (containing the moisturizing and soothing plant composition of Example 3) and Comparative Example 1 (containing the moisturizing and soothing plant composition of Comparative Example 5).

[0076] Eight volunteers who identified as having sensitive skin or experiencing skin discomfort within the past two weeks were randomly divided into two groups of four. Each group used the moisturizing and soothing spray from Application Example 1 and Comparative Example 1, respectively, for three days. Skin samples were collected from the volunteers before and after product use. The samples were taken using VISIA cameras to photograph the volunteers' faces and observe changes in the redness areas of the skin. Facial changes are shown below. Figure 6-7 As shown.

[0077] The above-mentioned 3-day test results show that after using the moisturizing and soothing spray of Application Example 1 (including the moisturizing and soothing plant composition of Example 3), ( Figure 6The volunteers' facial redness and inflammation improved, especially on the cheeks. Volunteers reported no irritation or stinging on broken skin and had a pleasant fragrance. However, after using the moisturizing and soothing spray (comparative example 1, containing the moisturizing and soothing plant composition of comparative example 5), (…) Figure 7 The volunteers' facial inflammation did not show significant improvement. This indicates that when Ningxia wolfberry extract, peony root extract, sophora root extract, chrysanthemum extract, and rhodiola rosea extract are used in combination within the component ratios specified in this invention, they have a better soothing effect on skin discomfort.

[0078] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A moisturizing and soothing plant composition, characterized in that, It includes the following components in parts by weight: 1-10 parts of Ningxia wolfberry extract, 1-5 parts of peony root extract, 0.5-7.5 parts of sophora root extract, 0.5-5 parts of Shennong chrysanthemum extract, and 0.05-0.5 parts of rhodiola rosea extract.

2. The moisturizing and soothing plant composition as described in claim 1, characterized in that, It includes the following components in parts by weight: 5-10 parts of Ningxia wolfberry extract, 3.5-5 parts of peony root extract, 7-7.5 parts of sophora root extract, 1.5-5 parts of Shennong chrysanthemum extract, and 0.2-0.5 parts of rhodiola rosea extract.

3. The moisturizing and soothing plant composition as described in claim 1, characterized in that, The mass ratio of the peony root extract and the sophora root extract is peony root extract: sophora root extract = 1: (1.5-2.5).

4. The moisturizing and soothing plant composition according to any one of claims 1-3, characterized in that, It also includes the following components in parts by weight: 20-25 parts butanediol, 12-15 parts glycerol, 0.2-0.4 parts p-hydroxyacetophenone, 0.5-1 part 1,2-hexanediol, and water to make up to 100 parts.

5. The use of the moisturizing and soothing plant composition according to any one of claims 1-4 in the preparation of moisturizing and soothing cosmetics.

6. The application as described in claim 5, characterized in that, The dosage forms of the cosmetics include water, lotion, and cream.

7. A moisturizing and soothing cosmetic product, characterized in that, Includes the moisturizing and soothing plant composition and excipients as described in any one of claims 1-4.

8. The moisturizing and soothing cosmetic product as described in claim 7, characterized in that, The excipients include at least one of the following: humectant, thickener, chelating agent, preservative, and water.

9. The moisturizing and soothing cosmetic product as described in claim 8, characterized in that, It includes the following components by weight percentage: 1-38.5% moisturizing and soothing plant composition, 1.1-36% humectant, 0.01-0.05% thickener, 0.01-1% chelating agent, 0.1-1% preservative, and the balance being deionized water.

10. The moisturizing and soothing cosmetic product as described in claim 8, characterized in that, The moisturizer includes at least one of panthenol, betaine, erythritol, sodium hyaluronate, butylene glycol, glycerin, propylene glycol, polyglycerol-10, and dipropylene glycol; And / or, the thickener includes at least one of xanthan gum, hydroxyethyl cellulose, and β-glucan; And / or, the chelating agent includes disodium EDTA; And / or, the preservative includes at least one of p-hydroxyacetophenone, methyl propylene glycol, capryloyl hydroxamic acid, glyceryl caprylate, 1,2-hexanediol, phenoxyethanol, and ethylhexylglycerin.

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