A traditional Chinese medicine composition capable of promoting bone healing

Abstract

The present application relates to the technical field of traditional Chinese medicine, and provides a traditional Chinese medicine composition capable of promoting bone fracture healing, which comprises the following components in parts by weight: Cordyceps militaris 25-35 parts, raspberry 20-25 parts, cucumber seed 15-22 parts, perilla seed 12-18 parts, radish seed 10-15 parts, bovine collagen peptide powder 8-12 parts, angelica 5-10 parts, and safflower 1-5 parts. The technical scheme solves the problem of poor effect of traditional Chinese medicine composition in promoting bone fracture healing.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine technology, specifically to a traditional Chinese medicine composition that can promote bone injury healing. Background Technology

[0002] Bone injuries (such as fractures, cracks, sprains, etc.) are common clinical conditions, mostly caused by external injury, strain or pathological factors that disrupt the integrity of bone structure. The healing process involves key steps such as improving local blood circulation, tissue repair and callus formation. During the healing period, patients often face problems such as local swelling and pain, slow tissue repair, poor callus growth or even delayed healing and nonunion. Poor healing can easily lead to sequelae such as joint dysfunction and chronic pain, which seriously affect the patient's quality of life.

[0003] Current clinical interventions for bone injury healing primarily focus on reduction and fixation, analgesia and anti-inflammation, and calcium or vitamin D supplementation. While these methods provide basic symptomatic treatment, some have limitations, such as insufficient specificity in promoting bone healing and the potential for long-term side effects (e.g., gastrointestinal irritation, drug dependence). In contrast, traditional Chinese medicine (TCM) offers unique advantages in bone injury treatment, including holistic regulation and fewer side effects. Through multi-component, multi-target action, it improves local microcirculation, inhibits inflammatory responses, and promotes osteoblast activity and bone matrix synthesis, thereby accelerating the bone healing process. This aligns with the holistic intervention needs of modern medicine for bone injury healing.

[0004] However, existing traditional Chinese medicine formulas for bone injury healing still have shortcomings, such as poor efficacy stability and slow onset of action, making it difficult to meet the clinical demand for rapid bone injury healing. Therefore, developing a scientifically formulated, safe, and effective traditional Chinese medicine composition that can specifically promote bone injury healing is of great significance for solving problems such as long healing cycles and poor efficacy in clinical bone injury treatment. Summary of the Invention

[0005] This invention proposes a traditional Chinese medicine composition that can promote bone injury healing, solving the problem that traditional Chinese medicine compositions in related technologies have poor effects on promoting bone injury healing.

[0006] The technical solution of the present invention is as follows: This invention proposes a traditional Chinese medicine composition that can promote bone injury healing. The raw materials of the composition include the following components in parts by weight: 25-35 parts of Cordyceps militaris, 20-25 parts of raspberry, 15-22 parts of cucumber seed, 12-18 parts of perilla seed, 10-15 parts of radish seed, 8-12 parts of bovine bone collagen peptide powder, 5-10 parts of angelica, and 1-5 parts of saffron.

[0007] This invention also proposes a method for preparing a traditional Chinese medicine composition that can promote bone injury healing, comprising the following steps: S1. Angelica sinensis powder is obtained by drying, pulverizing and sieving Angelica sinensis as raw material; S2. The Angelica powder and enzyme are added to a solvent and mixed, then enzymatically hydrolyzed and filtered to obtain filter residue and filtrate I; S3. The filter residue is subjected to alcohol extraction and filtration to obtain filtrate II; S4. After combining filtrate I and filtrate II, concentrate and dry to obtain Angelica sinensis extract; S5. After mixing Cordyceps militaris, raspberry, cucumber seeds, perilla seeds, radish seeds, bovine bone collagen peptide powder, and saffron, pulverize them, add Angelica sinensis extract, mix them, and then pulverize and sieve them to obtain a traditional Chinese medicine composition that can promote bone injury healing. In step S2, the enzymatic hydrolysis includes a first enzymatic hydrolysis and a second enzymatic hydrolysis, and the enzymes used in the first enzymatic hydrolysis and the second enzymatic hydrolysis are different.

[0008] Angelica sinensis, a traditional Chinese medicine, has long been recognized for its efficacy in promoting blood circulation, removing blood stasis, nourishing blood, and relieving pain. Modern pharmacological research has further confirmed that its active ingredients, such as ligustilide, can effectively improve local blood circulation, inhibit inflammation, and provide a favorable environment for bone injury healing, thus having significant application value in promoting bone injury healing. However, the extraction process of Angelica sinensis often suffers from low extraction rates and insufficient purity of active ingredients. Therefore, this invention improves the preparation process of Angelica sinensis extract, increasing the content of ligustilide in the extract. The resulting extract can more fully exert its effects of improving local circulation and delivering nutrients in the traditional Chinese medicine composition. Furthermore, its synergistic effect with components such as Cordyceps militaris and bovine bone collagen peptide powder significantly enhances the overall efficacy of the composition in promoting bone injury healing.

[0009] As a further technical solution, the enzyme used for enzymatic hydrolysis is 0.3% of the mass of Angelica sinensis powder.

[0010] As a further technical solution, in step S1, the sieving is performed through a 100-mesh sieve.

[0011] As a further technical solution, in step S2, the mass ratio of the Angelica sinensis powder to the solvent is 1:5.

[0012] As a further technical solution, in step S2, the solvent is an aqueous solution of glacial acetic acid with a pH of 5.

[0013] As a further technical solution, the enzymes used in the first enzymatic hydrolysis consist of cellulase and pectinase, and the enzymes used in the second enzymatic hydrolysis consist of acidic amylase and xylanase.

[0014] This invention employs a two-stage enzymatic hydrolysis process using different enzymes. The first stage utilizes the synergistic action of cellulase and pectinase to effectively disrupt the cell wall structure of Angelica sinensis, promoting cell wall decomposition and facilitating the release of intracellular active ingredients. The second stage employs acidic amylase and xylanase to effectively degrade impurities such as starch and xylan in the system, reducing the encapsulation and interference of impurities with the active ingredients. The synergistic effect of these two enzymatic hydrolysates further improves the yield and purity of the Angelica sinensis extract. As a further technical solution, the mass ratio of cellulase to pectinase in the first enzymatic hydrolysis process is 7:7~9.

[0015] As a further technical solution, the mass ratio of xylanase to acid amylase in the second enzymatic hydrolysis process is 1:2~3.

[0016] As a further technical solution, the specific steps in step S2 are as follows: the angelica powder, cellulase and pectinase are added to a solvent and mixed, and after the first enzymatic hydrolysis, acidic amylase and xylanase are added for the second enzymatic hydrolysis, and then filtered to obtain filter residue and filtrate I.

[0017] As a further technical solution, the first enzymatic hydrolysis is performed at a temperature of 50-60°C for 3 hours; the second enzymatic hydrolysis is performed at a temperature of 55°C for 3 hours.

[0018] As a further technical solution, in step S3, the alcohol extraction is an alcohol reflux extraction, and the solvent used in the alcohol extraction consists of isopropanol and ethanol.

[0019] As a further technical solution, in step S3, the alcohol extraction includes a first alcohol extraction and a second alcohol extraction.

[0020] As a further technical solution, during the first alcohol extraction, the mass ratio of isopropanol to ethanol is 1:3~5, preferably 1:3.

[0021] As a further technical solution, during the second alcohol extraction, the mass ratio of isopropanol to ethanol is 3~5:1, preferably 3:1.

[0022] As a further technical solution, the ethanol is a 75wt% aqueous solution of ethanol.

[0023] As a further technical solution, the isopropanol is a 70% aqueous solution of isopropanol.

[0024] The present invention also proposes a traditional Chinese medicine decoction piece, the preparation method of which includes the following steps: adding sorbitol to the traditional Chinese medicine composition for promoting bone injury healing, adding an aqueous ethanol solution for granulation, drying, sizing, adding magnesium stearate for mixing, and then compressing into tablets to obtain the decoction piece.

[0025] The working principle and beneficial effects of this invention are as follows: This invention provides a traditional Chinese medicine composition that can promote bone injury healing. By rationally combining various traditional Chinese medicine compositions, the effect of promoting bone injury healing is improved. In this invention, bovine bone collagen peptide powder can directly provide small molecule raw materials for bone matrix synthesis, promoting osteoblast proliferation and callus formation; Angelica sinensis extract can invigorate blood circulation and remove blood stasis, improving microcirculation at the bone injury site to eliminate stagnation, and the two together serve as the principal drugs to promote bone repair; Cordyceps militaris can nourish the lungs and kidneys, enhance the body's resistance to avoid delayed healing, saffron strengthens blood circulation and removes blood stasis to relieve pain, cucumber seeds supplement the minerals needed for bone and promote bone calcium deposition, and the three serve as the assistant drugs to assist the principal drugs in enhancing the healing effect; raspberry assists in bone strengthening, perilla seeds and radish seeds regulate qi and bowel movement, improve digestion, and together serve as the adjuvant drugs to harmonize the functions of the internal organs and the excessive properties of the drugs; fructooligosaccharides regulate the intestinal flora to promote the absorption of effective ingredients and improve taste, xylitol further harmonizes the bitter taste, and selenium-enriched rye germ peptide powder supplements selenium and enhances antioxidant capacity, and the three serve as the guiding drugs to harmonize the whole body. Through the reasonable compatibility of each component, the synergistic effect of strengthening bones, promoting qi and blood circulation, supporting the body's resistance, and regulating the internal organs is achieved, thereby effectively promoting bone injury healing. Detailed Implementation

[0026] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0027] In the following embodiments and comparative examples: Cellulase, model: SPE-013, manufacturer: Ningxia Xiasheng Industrial Group Co., Ltd., enzyme activity: 4000μ / g; Pectinase, model: SPE-010, manufacturer: Ningxia Xiasheng Industrial Group Co., Ltd., enzyme activity: 5000u / g; Acid amylase, model: FDY-2218, manufacturer: Ningxia Xiasheng Industrial Group Co., Ltd., enzyme activity: 700,000 u / mL; Xylanase, model: FFG-0652, manufacturer: Ningxia Xiasheng Industrial Group Co., Ltd., enzyme activity: 110,000 u / g; Acidic protease, model: FDY-2205, manufacturer: Ningxia Xiasheng Industrial Group Co., Ltd., enzyme activity 150,000 u / mL; Glacial acetic acid is an aqueous solution of glacial acetic acid with a pH of 5; The ethanol is a 75% (w / w) aqueous solution. The isopropanol is a 70% (w / w) aqueous solution. The following formula is used to calculate the extraction rate of ligustilide: Extraction rate of ligustilide (%) = (mass of Angelica sinensis extract × content of ligustilide in Angelica sinensis extract) / mass of ligustilide in Angelica sinensis * 100.

[0028] Example 1 A method for preparing a traditional Chinese medicine composition that can promote bone injury healing includes the following steps: S1. By weight, 5 parts of Angelica sinensis are dried, pulverized, and passed through a 100-mesh sieve to obtain Angelica sinensis powder; S2. After mixing Angelica powder with glacial acetic acid, add cellulase and pectinase in a mass ratio of 1:1 (the sum of the mass of cellulase and pectinase is 0.3% of the mass of Angelica powder) and mix. Enzymatically hydrolyze at 50℃ for 6 hours, filter, and obtain filter residue and filtrate I, wherein the mass ratio of Angelica powder to glacial acetic acid is 1:5. S3. Add 6 times the mass of isopropanol and ethanol in a mass ratio of 1:3 to the filter residue and reflux for 2 hours. Filter to obtain filtrate II. S4. After combining filtrate I and filtrate II, the extract was concentrated and dried to obtain Angelica sinensis extract, in which the extraction rate of ligustilide was 87.1%. S5. Mix 25 parts of Cordyceps militaris, 20 parts of raspberry, 15 parts of cucumber seed, 12 parts of perilla seed, 10 parts of radish seed, 8 parts of bovine bone collagen peptide powder, and 1 part of saffron. Grind the mixture to 100 mesh, then add Angelica sinensis extract, mix evenly, grind, and pass through a 150-mesh sieve to obtain a traditional Chinese medicine composition that can promote bone injury healing.

[0029] Example 2 A method for preparing a traditional Chinese medicine composition that can promote bone injury healing includes the following steps: S1. By weight, 8 parts of Angelica sinensis are dried, pulverized, and passed through a 100-mesh sieve to obtain Angelica sinensis powder; S2. After mixing Angelica powder with glacial acetic acid, add cellulase and pectinase in a mass ratio of 1:1 (the total mass of cellulase and pectinase is 0.3% of the mass of Angelica powder) and mix. Enzymatically hydrolyze at 55℃ for 6 hours, filter, and obtain filter residue and filtrate I, wherein the mass ratio of Angelica powder to glacial acetic acid is 1:5. S3. Add 7 times the mass of isopropanol and ethanol in a mass ratio of 1:3 to the filter residue and reflux for 2 hours. Filter to obtain filtrate II. S4. After combining filtrate I and filtrate II, the extract was concentrated and dried to obtain Angelica sinensis extract, in which the extraction rate of ligustilide was 87.6%. S5. Mix 30 parts of Cordyceps militaris, 23 parts of raspberry, 18 parts of cucumber seed, 16 parts of perilla seed, 12 parts of radish seed, 10 parts of bovine bone collagen peptide powder, and 3 parts of saffron. Grind the mixture to 100 mesh. Then add 12 parts of Angelica sinensis extract, mix well, grind, and pass through a 150-mesh sieve to obtain a traditional Chinese medicine composition that can promote bone injury healing.

[0030] Example 3 A method for preparing a traditional Chinese medicine composition that can promote bone injury healing includes the following steps: S1. By weight, 10 parts of Angelica sinensis are dried, pulverized, and passed through a 100-mesh sieve to obtain Angelica sinensis powder; S2. After mixing Angelica powder with glacial acetic acid, add cellulase and pectinase in a mass ratio of 1:1 (the sum of the mass of cellulase and pectinase is 0.3% of the mass of Angelica powder) and mix. Enzymatically hydrolyze at 60℃ for 6 hours, filter, and obtain filter residue and filtrate I, wherein the mass ratio of Angelica powder to glacial acetic acid is 1:5. S3. Add 8 times the amount of isopropanol and ethanol in a mass ratio of 1:3 to the filter residue and reflux for 2 hours. Filter to obtain filtrate II. S4. After combining filtrate I and filtrate II, the extract was concentrated and dried to obtain Angelica sinensis extract, in which the extraction rate of ligustilide was 86.5%. S5. Mix 35 parts of Cordyceps militaris, 25 parts of raspberry, 22 parts of cucumber seed, 18 parts of perilla seed, 15 parts of radish seed, 12 parts of bovine bone collagen peptide powder, and 5 parts of saffron, then pulverize to 100 mesh. Add 15 parts of Angelica sinensis extract, mix evenly, pulverize, and pass through a 150-mesh sieve to obtain a traditional Chinese medicine composition that can promote bone injury healing.

[0031] Example 4 The only difference between this embodiment and Embodiment 2 is that, in step S2, cellulase and pectinase are replaced with equal amounts of xylanase and acid amylase in a mass ratio of 1:2. Results: The extraction rate of ligustilide was 84.1%.

[0032] Example 5 The difference between this embodiment and Embodiment 2 is only that in step S2, after adding Angelica sinensis powder to glacial acetic acid and mixing, cellulase and pectinase in a mass ratio of 1:1 (the sum of the mass of cellulase and pectinase is 0.2% of the mass of Angelica sinensis powder) are added and mixed. The mixture is then enzymatically hydrolyzed at 50°C for 3 hours to obtain the first enzymatic hydrolysate. Xylanase and acid amylase in a mass ratio of 1:2 (the sum of the mass of xylanase and acid amylase is 0.1% of the mass of Angelica sinensis powder) are added to the first enzymatic hydrolysate and mixed. The mixture is then enzymatically hydrolyzed at 55°C for 3 hours to obtain the second enzymatic hydrolysate. The mixture is then filtered to obtain filter residue and filtrate I. Results: The extraction rate of ligustilide was 91.2%.

[0033] Example 6 The difference between this embodiment and Embodiment 2 is only that in step S2, after adding Angelica sinensis powder to glacial acetic acid and mixing, xylanase and acid amylase with a mass ratio of 1:2 (the total mass of xylanase and acid amylase is 0.1% of the mass of Angelica sinensis powder) are added and mixed. The mixture is then enzymatically hydrolyzed at 50°C for 3 hours to obtain the first enzymatic hydrolysate. Cellulase and pectinase with a mass ratio of 1:1 (the total mass of cellulase and pectinase is 0.2% of the mass of Angelica sinensis powder) are added to the first enzymatic hydrolysate and mixed. The mixture is then enzymatically hydrolyzed at 55°C for 3 hours to obtain the second enzymatic hydrolysate. The mixture is then filtered to obtain filter residue and filtrate I. Results: The extraction rate of ligustilide was 89.5%.

[0034] Example 7 The only difference between this embodiment and Embodiment 2 is that the cellulase and pectinase in a mass ratio of 1:1 are replaced with equal amounts of xylanase, acid amylase, cellulase and pectinase in a mass ratio of 2:4:3:3.

[0035] Results: The extraction rate of ligustilide was 90.1%.

[0036] Example 8 The only difference between this embodiment and Embodiment 2 is that cellulase is replaced with an equal amount of pectinase. Results: The extraction rate of ligustilide was 83.0%.

[0037] Example 9 The only difference between this embodiment and Embodiment 2 is that pectinase is replaced with an equal amount of cellulase. Results: The extraction rate of ligustilide was 82.7%.

[0038] Example 10 The only difference between this embodiment and Embodiment 2 is that cellulase is replaced with acidic protease.

[0039] Results: The extraction rate of ligustilide was 82.1%.

[0040] Example 11 The difference between this embodiment and embodiment 5 is that in step S3, isopropanol and ethanol in a mass ratio of 1:3 (7 times the mass of the filter residue) are added to the filter residue and refluxed for 1 hour, filtered to obtain the first extract and the first filter residue. Methanol and ethanol in a mass ratio of 3:1 (7 times the mass of the filter residue) are added to the first filter residue and refluxed for 1 hour, filtered to obtain the second extract and the second filter residue. The first extract and the second extract are combined to obtain filtrate II. Results: The extraction rate of ligustilide was 95.2%.

[0041] Example 12 The difference between this embodiment and embodiment 5 is that in step S3, 7 times the amount of isopropanol and ethanol in a mass ratio of 3:1 are added to the filter residue and refluxed for 1 hour, filtered to obtain the first extract and the first filter residue. 7 times the amount of methanol and ethanol in a mass ratio of 1:3 are added to the first filter residue and refluxed for 1 hour, filtered to obtain the second extract and the second filter residue. The first extract and the second extract are combined to obtain filtrate II. Results: The extraction rate of ligustilide was 91.0%.

[0042] Example 13 The only difference between Example 1 and Example 5 is that the methanol and ethanol in a mass ratio of 1:3 are replaced with equal amounts of isopropanol and ethanol in a mass ratio of 3:1.

[0043] Results: The extraction rate of ligustilide was 90.5%.

[0044] The extraction rates of ligustilide in Examples 5-6 were higher than those in Examples 1-4 and Examples 7-10, indicating that the present invention improved the extraction rate of ligustilide through two enzymatic hydrolysis processes. The extraction rate of ligustilide in Example 11 was higher than that in Examples 5 and Examples 12-13, indicating that the present invention first extracted with isopropanol and ethanol at a mass ratio of 1:3, and then further improved the extraction rate of ligustilide by extraction with isopropanol and ethanol at a mass ratio of 3:1.

[0045] Experiment Example 1: Trial use in human population 1.1 Preparation method of therapeutic drugs After mixing 60 parts of the traditional Chinese medicine composition obtained in Example 9, 21.5 parts of sorbitol, 10 parts of microcrystalline cellulose and 8 parts of maltodextrin were added and mixed. Then, 25 parts of 50% ethanol aqueous solution were added to granulate, dry and granulate. After adding 0.5 parts of magnesium stearate, the mixture was compressed into tablets to obtain medicinal slices (each tablet contains 0.8g, and 6 tablets need to be taken daily).

[0046] 120 patients with bone injuries were randomly selected. Among them, there were 60 males and 60 females. The ages of the patients were as follows: 20 patients aged 6 to 15 years, 20 patients aged 16 to 25 years, 20 patients aged 26 to 35 years, 20 patients aged 36 to 45 years, 20 patients aged 46 to 55 years, and 20 patients aged 56 to 65 years.

[0047] Medication Timing: For bone injury patients who do not require surgical incision and fixation, medication should begin after X-ray examination confirms that the bone's repositioning and fixation stability have reached clinically satisfactory standards following splinting or plaster cast immobilization. One course of treatment is 28 days. The results after 28 days are as follows: Group aged 6 to 15 years: 20 cases were effective, 0 cases were significantly effective, and 0 cases were ineffective.

[0048] In the 16-25 age group: 18 cases were effective, 2 cases were significantly effective, and 0 cases were ineffective.

[0049] In the 26-35 age group: 19 cases were effective, 1 case was significantly effective, and 0 cases were ineffective.

[0050] In the 36-45 age group: 17 cases were effective, 2 cases were significantly effective, and 1 case was ineffective.

[0051] In the 46-55 age group: 15 cases were effective, 3 cases were significantly effective, and 2 cases were ineffective.

[0052] In the 56-65 age group: 13 cases were effective, 5 cases were significantly effective, and 2 cases were ineffective.

[0053] Among them, effective is defined as follows: after one course of treatment, imaging shows that the callus has basically formed, the fracture is well aligned, and mild movement is possible, and the swelling and pain of the affected limb have disappeared; markedly effective is defined as following one course of oral administration, imaging shows that the callus has formed continuously, the alignment is well, and mild movement is possible, and the swelling and pain of the affected limb have basically disappeared; ineffective is defined as following one course of oral administration, there is no change in signs and symptoms.

[0054] According to the experimental results, the effective and significant efficacy rate in treating patients with bone injuries reached 95%. The traditional Chinese medicine composition of this invention has a certain effect on promoting bone injury healing.

[0055] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition that can promote bone injury healing, characterized in that, The raw materials of the composition include the following components in parts by weight: 25-35 parts of Cordyceps militaris, 20-25 parts of raspberry, 15-22 parts of cucumber seed, 12-18 parts of perilla seed, 10-15 parts of radish seed, 8-12 parts of bovine bone collagen peptide powder, 5-10 parts of angelica, and 1-5 parts of saffron.

2. A method for preparing a traditional Chinese medicine composition that can promote bone injury healing, used to prepare the traditional Chinese medicine composition that can promote bone injury healing as described in claim 1, characterized in that, Includes the following steps: S1. Angelica sinensis powder is obtained by drying, pulverizing and sieving Angelica sinensis as raw material; S2. The Angelica powder and enzyme are added to a solvent and mixed, then enzymatically hydrolyzed and filtered to obtain filter residue and filtrate I; S3. The filter residue is subjected to alcohol extraction and filtration to obtain filtrate II; S4. After combining filtrate I and filtrate II, concentrate and dry to obtain Angelica sinensis extract; S5. After mixing Cordyceps militaris, raspberry, cucumber seeds, perilla seeds, radish seeds, bovine bone collagen peptide powder, and saffron, pulverize them, add Angelica sinensis extract, mix evenly, and then pulverize and sieve to obtain a traditional Chinese medicine composition that can promote bone injury healing. In step S2, the enzymatic hydrolysis includes a first enzymatic hydrolysis and a second enzymatic hydrolysis, and the enzymes used in the first enzymatic hydrolysis and the second enzymatic hydrolysis are different.

3. The method for preparing a traditional Chinese medicine composition that can promote bone injury healing according to claim 2, characterized in that, In step S1, the sieving is done through a 100-mesh sieve.

4. The method for preparing a traditional Chinese medicine composition that can promote bone injury healing according to claim 2, characterized in that, In step S2, the solvent is an aqueous solution of glacial acetic acid with a pH of 5.

5. A method for preparing a traditional Chinese medicine composition that can promote bone injury healing according to claim 2, characterized in that, The enzymes used in the first enzymatic hydrolysis consist of cellulase and pectinase, while the enzymes used in the second enzymatic hydrolysis consist of acidic amylase and xylanase.

6. A method for preparing a traditional Chinese medicine composition that can promote bone injury healing according to claim 5, characterized in that, The mass ratio of cellulase to pectinase in the first enzymatic hydrolysis process is 7:7~9.

7. A method for preparing a traditional Chinese medicine composition that can promote bone injury healing according to claim 5, characterized in that, In the second enzymatic hydrolysis process, the mass ratio of xylanase to acid amylase is 1:2~3.

8. A method for preparing a traditional Chinese medicine composition that can promote bone injury healing according to claim 5, characterized in that, The specific steps in step S2 are as follows: the angelica powder, cellulase and pectinase are added to a solvent and mixed. After the first enzymatic hydrolysis, acidic amylase and xylanase are added for the second enzymatic hydrolysis. After filtration, filter residue and filtrate I are obtained.

9. A method for preparing a traditional Chinese medicine composition that can promote bone injury healing according to claim 8, characterized in that, The first enzymatic hydrolysis was performed at a temperature of 50-60°C for 3 hours; the second enzymatic hydrolysis was performed at a temperature of 55°C for 3 hours.

10. A method for preparing a traditional Chinese medicine composition that can promote bone injury healing according to claim 2, characterized in that, In step S3, the alcohol extraction is an alcohol reflux extraction, and the solvent used in the alcohol extraction consists of isopropanol and ethanol.