Process for efficiently extracting anthocyanin from lycium ruthenicum

By using a compound enzyme system and a gradient fermentation strategy, the problem of low extraction rates of anthocyanins and total alkaloids in black goji berries was solved, and the content of anthocyanins and total alkaloids in the extract was increased simultaneously, meeting the high added value requirements of industrial production.

CN122168700APending Publication Date: 2026-06-09BEIJING GUOHUA XINYE TRADITIONAL CHINESE MEDICINE RES INST CO LTD
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Patent Information

Application Number
CN202610234554.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-02-27
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing technologies have failed to design a specific anthocyanin extraction scheme for black goji berries, and cannot simultaneously increase the content of total alkaloids and anthocyanins in the extract, making it difficult to meet the high added value requirements of industrial production.

Method used

A complex enzyme system (cellulase, pectinase, and β-glucosidase) was used to synergistically break down cell walls. Combined with a gradient fermentation strategy of first aerobic and then anaerobic fermentation, a complex strain of Aspergillus niger, Lactobacillus rhamnosus, and Lactobacillus paracasei was used to simultaneously extract anthocyanins and total alkaloids through enzymatic hydrolysis and fermentation.

Benefits of technology

It significantly increased the content of anthocyanins and total alkaloids in black goji berry extract, thereby enhancing the overall physiological activity of the extract and meeting the high added value requirements of industrial production.

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Abstract

The invention belongs to the technical field of plant effective component extraction, and particularly relates to a process for efficiently extracting anthocyanin from lycium ruthenicum. The process comprises the following steps: step 1, preparing lycium ruthenicum powder; step 2, adding a buffer solution into the lycium ruthenicum powder to obtain a lycium ruthenicum suspension; adding a compound enzyme into the lycium ruthenicum suspension, and carrying out enzymolysis, enzyme deactivation and cooling to obtain an enzymatic hydrolysate; 3, aspergillus niger is added into the enzymatic hydrolysate, fermentation and sterilization are performed, and primary fermentation liquor is obtained; compound bacteria are continuously added, anaerobic fermentation, sterilization and cooling are performed, and final fermentation liquor is obtained; step 4, performing alcohol extraction on the final fermentation liquor, and removing ethanol to obtain concentrated crude extract; and 5, purifying the concentrated crude extract by using AB-8 macroporous adsorption resin, and drying to obtain the lycium ruthenicum extract. According to the process disclosed by the invention, the extraction rates of total alkaloids and anthocyanin in the lycium ruthenicum extract can be synchronously increased.
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