Sarcoma fusion gene nanopore sequencing primer group and application thereof

By designing standardized nanopore sequencing primer sets and matching kits for sarcoma fusion genes, the problems of poor primer specificity and low amplification efficiency in existing technologies have been solved, enabling efficient and accurate detection of sarcoma fusion genes and supporting early diagnosis and targeted therapy.

CN122168759APending Publication Date: 2026-06-09SHANGHAI SIXTH PEOPLES HOSPITAL
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Patent Information

Application Number
CN202610553985.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-24
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing methods for detecting sarcoma fusion genes suffer from poor primer specificity, low amplification efficiency, lack of standardized design systems, and a shortage of alternative primers, resulting in high detection costs and insufficient sensitivity, making it difficult to meet diverse clinical needs.

Method used

A set of nanopore sequencing primers for sarcoma fusion genes with high specificity and excellent amplification performance, following the principle of standardization, was designed. It includes 5 pairs of fixed sequence primers with a length of 18-25 bp, a GC content of 40%-60%, and a 3' end that strictly matches the target sequence. The length of the amplified product is adapted to nanopore sequencing, and the matching kit achieves full-process standardization.

Benefits of technology

This method enables the detection of sarcoma fusion genes with high specificity and high amplification efficiency, simplifies the experimental procedure, and improves the accuracy and universality of detection, providing a reliable molecular biological basis for the early diagnosis and targeted therapy of sarcoma.

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Abstract

The invention relates to a sarcoma fusion gene nanopore sequencing primer group and application thereof, the primer group comprises five pairs of specific primers (Primer 1-5) with fixed sequences, each primer follows the design principle that the length is 18-25 bp, the GC content is 40%-60%, and the 3'end is strictly matched with a target sequence, and the length of an amplification product is 300-800 bp to adapt to nanopore sequencing. Through qPCR verification, melting curves of the primer group are all single-peak, the amplification efficiency is close to 100%, the specificity is high, and the amplification performance is excellent.
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