A composition and method for increasing energy conversion efficiency of rhodamine b in pure water

Abstract

The present application belongs to the field of laser devices and technology, and particularly relates to a composition and method for improving energy conversion efficiency of rhodamine B in pure water, which comprises rhodamine B, sulfobutyl-beta-cyclodextrin, and the molar ratio of rhodamine B to sulfobutyl-beta-cyclodextrin is 1:0.9-1.1. According to the molecular structure of sulfobutyl-beta-cyclodextrin, a hydrophobic cavity and a ring-mounted main body with good water solubility are constructed, and the inclusion complexation and molecular recognition mechanism are utilized to accommodate rhodamine B dye molecules, effectively solving the aggregation effect of fluorescent dye molecules in pure water medium, and overcoming the problem of introduction of organic solvents (hazardous chemicals) in the prior art. The method can realize high-efficiency energy conversion output of rhodamine B dye in pure water medium.

Description

Technical Field

[0001] This invention belongs to the field of laser devices and technology, specifically relating to a composition and method for improving the energy conversion efficiency of Rhodamine B in pure water. Background Technology

[0002] Rhodamine B dye is an important optical material and is widely used in many fields, including dye oscillator amplifiers, quantum counters, photosensitizers, spectral calibration, single-molecule detection, bioimaging, and scanning confocal microscopy.

[0003] However, because Rhodamine B dye exhibits a strong tendency to aggregate in pure water, resulting in low fluorescence output and low energy conversion efficiency, it usually needs to be dissolved in organic solvents during its application. However, as a hazardous chemical, the introduction of organic reagents will bring certain safety risks and additional costs to its application process, especially in various stages of industrial production, leading to significant environmental impact and high safety risks.

[0004] Therefore, there is an urgent need to develop a method to improve the energy conversion efficiency of fluorescein B dye in pure water media. Summary of the Invention

[0005] The purpose of this invention is to provide a composition and method for improving the energy conversion efficiency of Rhodamine B in pure water. Based on the sulfobutyl-β-cyclodextrin molecule, a hydrophobic, porous, and water-soluble ring-based macromolecular structure is constructed. Utilizing its inclusion complexation and molecular recognition mechanisms, Rhodamine B dye molecules are accommodated within this structure, effectively solving the aggregation effect of fluorescent dye molecules in pure water and overcoming the problem of introducing organic solvents (hazardous chemicals) in existing technologies. Applying the method of this invention enables high-efficiency energy conversion output of Rhodamine B dye in pure water.

[0006] Technical solution to achieve the purpose of this invention: A composition for improving the energy conversion efficiency of Rhodamine B in pure water, comprising Rhodamine B and sulfobutyl-β-cyclodextrin, wherein the molar ratio of Rhodamine B to sulfobutyl-β-cyclodextrin is 1:0.9-1.1.

[0007] A method for improving the energy conversion efficiency of Rhodamine B in pure water includes: S1: Add sulfobutyl-β-cyclodextrin to pure water and dissolve it to obtain a pure aqueous solution containing sulfobutyl-β-cyclodextrin; S2: Add Rhodamine B to a pure aqueous solution containing sulfobutyl-β-cyclodextrin and continue to dissolve to obtain a pure aqueous solution containing sulfobutyl-β-cyclodextrin-Rhodamine B; S3: Place a pure aqueous solution containing sulfobutyl-β-cyclodextrin-rhodamine B into the optical system and measure the energy conversion efficiency.

[0008] Furthermore, the molar ratio of sulfobutyl-β-cyclodextrin added in S1 to rhodamine B added in S2 is: rhodamine B: sulfobutyl-β-cyclodextrin = 1: 0.9-1.1.

[0009] Furthermore, the dissolution temperature in S1 is 20℃-30℃, and the time is 2-3 h.

[0010] Furthermore, the dissolution method in S1 is ultrasonic oscillation or cyclic stirring.

[0011] Furthermore, the dissolution temperature in S2 is 20℃-30℃, and the time is 2-3 h.

[0012] Furthermore, the dissolution method in S2 is ultrasonic oscillation or cyclic stirring.

[0013] The beneficial technical effects of this invention are as follows: This invention utilizes sulfobutyl-β-cyclodextrin cyclic macromolecules to construct a water-soluble cyclic host system, forming a supramolecular non-covalent complex between Rhodamine B and the cyclic macromolecules. This promotes the depolymerization of Rhodamine B dye molecules in pure water, thereby significantly improving the energy conversion efficiency of Rhodamine B dye molecules in pure water. This avoids the introduction of hazardous organic chemicals during the use of fluorescent dyes, improves the economic benefits of use, and reduces safety risks during use. Attached Figure Description

[0014] Figure 1 This is a schematic diagram illustrating the binding energy and binding mode of Rhodamine B molecule (fluorescent dye) and sulfobutyl-β-cyclodextrin molecule (cyclic host molecule) provided in Example 1 of the present invention. Figure 1 In the diagram, A and B are simulated phase diagrams of the composition, and B is the phase diagram of A after rotating it by 180°. Figure 2 The fluorescence spectra of Rhodamine B aqueous solution before and after the addition of sulfobutyl-β-cyclodextrin provided in Example 1 of this invention are shown. Detailed Implementation

[0015] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments.

[0016] This invention provides a composition for improving the energy conversion efficiency of Rhodamine B in pure water, comprising Rhodamine B and sulfobutyl-β-cyclodextrin, wherein the molar ratio of Rhodamine B to sulfobutyl-β-cyclodextrin is 1:0.9-1.1.

[0017] This invention also provides a method for improving the energy conversion efficiency of Rhodamine B in pure water, specifically including the following steps: S1: Add sulfobutyl-β-cyclodextrin to pure water and dissolve it by ultrasonic oscillation or cyclic stirring to obtain a pure aqueous solution containing sulfobutyl-β-cyclodextrin. Based on the pure water volume of the storage device and the concentration of the fluorescent dye used in the application scenario of Rhodamine B dye molecules, the required mass of the fluorescent dye is calculated. Sulfobutyl-β-cyclodextrin cyclic macromolecular dye additive with a molar ratio of Rhodamine B to sulfobutyl-β-cyclodextrin of 1:0.9-1.1 is added to pure water, and the mixture is dissolved by ultrasonic oscillation or cyclic stirring at 20℃-30℃ for 2-3 h to obtain a pure aqueous solution containing sulfobutyl-β-cyclodextrin. S2: Add Rhodamine B to a pure aqueous solution containing sulfobutyl-β-cyclodextrin, and continue ultrasonic oscillation or cyclic stirring to dissolve, thereby obtaining a pure aqueous solution containing sulfobutyl-β-cyclodextrin-Rhodamine B. Add the required mass of Rhodamine B dye to the pure aqueous solution containing the above-mentioned sulfobutyl-β-cyclodextrin cyclic macromolecular dye additive, and continue to dissolve it by ultrasonic oscillation or cyclic stirring at 20℃-30℃ for 2-3 hours. S3: Place a pure aqueous solution containing sulfobutyl-β-cyclodextrin-rhodamine B into the optical system and measure the energy conversion efficiency. Place the pure aqueous solution containing the sulfobutyl-β-cyclodextrin-rhodamine B dye molecular complex system into the optical system, and start the system operation according to the system working mode.

[0018] Example 1 This embodiment provides a method to improve the energy conversion efficiency of Rhodamine B in pure water, using a molar ratio of Rhodamine B to sulfobutyl-β-cyclodextrin of 1:1. The method specifically includes the following steps: S1: Add 2.54g of sulfobutyl-β-cyclodextrin (average molecular weight 1217g / mol) to a wide-mouth bottle containing 10L of pure water. Place the wide-mouth bottle into an ultrasonic cleaner and dissolve it by ultrasonic vibration at 30℃ for 2 hours. S2: Add 1g of Rhodamine B fluorescent dye (molecular weight 479g / mol) to the wide-mouth bottle and continue to dissolve it by ultrasonic vibration at 30℃ for 2h. S3: Place the dissolved (sulfobutyl-β-cyclodextrin)-rhodamine B complex aqueous solution from the wide-mouth bottle into the circulation pump of the fifth stage optical amplifier of the MOPA oscillating amplifier chain, and start the system according to the system's operating requirements.

[0019] S4: Measure and calculate the energy conversion efficiency of the amplification stage, which is 20.62%. The energy conversion efficiency of the amplification stage without step S1 is only 11.5%.

[0020] S5: Using the BSSE (basis set overlap error) method with the def2-TZVP basis set and applying D3 empirical dispersion correction (with Becke–Johnson damping), the structure and energy of the rhodamine B and sulfobutyl-β-cyclodextrin complex system were calculated. The interactions of the system were further analyzed using the IGMH (independent gradient model based on HirshMnld segmentation) in the Multiwfn software package. The binding energy and binding mode of the rhodamine B and sulfobutyl-β-cyclodextrin molecules were calculated. The results are as follows: Figure 1 As shown.

[0021] Figure 1 The results showed that the carboxyl group of the Rhodamine B molecule can form hydrogen bonds with the two hydroxyl groups of the sugar ring of the sulfobutyl-β-cyclodextrin molecule; the benzene ring of the Rhodamine B molecule penetrates deep into the cavity of the sulfobutyl-β-cyclodextrin molecule and forms a hydrophobic interaction with the cavity of the sugar ring; the two N,N-diethylamino groups of the Rhodamine B molecule, which are positively charged, form salt bridges with the negatively charged sulfonic acid groups at the end of the side chain of the sulfobutyl-β-cyclodextrin molecule; the overall binding energy is -32.70 Kcal / mol.

[0022] S6: Measure the fluorescence spectra of Rhodamine B aqueous solution before and after the addition of sulfobutyl-β-cyclodextrin. The results are as follows: Figure 2 As shown, after adding sulfobutyl-β-cyclodextrin, the central wavelength of the solution was 594.20 nm, a significant red shift compared to 591.52 nm for the pure Rhodamine B aqueous solution. This red shift in the emission spectrum also indicates, to some extent, the formation of an ion-coupled interaction between the Rhodamine B molecule and the sulfonate groups of the sulfobutyl-β-cyclodextrin matrix. Furthermore, the fluorescence intensity of the Rhodamine B aqueous solution increased from 0.87 to 0.93 after the addition of sulfobutyl-β-cyclodextrin, indicating a certain degree of improvement in the luminescent performance of the Rhodamine B fluorescent dye.

[0023] Example 2 This embodiment provides a method for improving the energy conversion efficiency of Rhodamine B in pure water, using a molar ratio of Rhodamine B to sulfobutyl-β-cyclodextrin of 1:0.95. The method specifically includes the following steps: S1: Fill the third stage of the MOPA resonant chain with 20L of pure water, then add 4.85g of sulfobutyl-β-cyclodextrin (average molecular weight 1217g / mol), turn on the circulation pump, and circulate and stir to dissolve for 3h at 20℃. S2: Continue to add 2g of Rhodamine B fluorescent dye (molecular weight 479g / mol) into the circulating pump and continue to circulate and stir to dissolve for 3h at 20℃; S3: Start the system operation of this amplification stage according to the system's operating requirements; S4: The energy conversion efficiency of this amplification stage was measured and calculated to be 22.12%, compared to only 14.04% without the addition of sulfobutyl-β-cyclodextrin.

[0024] The present invention has been described in detail above with reference to the accompanying drawings and embodiments. However, the present invention is not limited to the above embodiments, and various changes can be made within the scope of knowledge possessed by those skilled in the art without departing from the spirit of the present invention. All contents not described in detail in the present invention can be derived from existing technologies.

Claims

1. A composition for improving the energy conversion efficiency of Rhodamine B in pure water, characterized in that, It includes rhodamine B and sulfobutyl-β-cyclodextrin, with a molar ratio of rhodamine B to sulfobutyl-β-cyclodextrin of 1:0.9-1.

1.

2. A method for improving the energy conversion efficiency of Rhodamine B in pure water, characterized in that, include: S1: Add sulfobutyl-β-cyclodextrin to pure water and dissolve it to obtain a pure aqueous solution containing sulfobutyl-β-cyclodextrin; S2: Add Rhodamine B to a pure aqueous solution containing sulfobutyl-β-cyclodextrin and continue to dissolve to obtain a pure aqueous solution containing sulfobutyl-β-cyclodextrin-Rhodamine B; S3: Place a pure aqueous solution containing sulfobutyl-β-cyclodextrin-rhodamine B into the optical system and measure the energy conversion efficiency.

3. The method for improving the energy conversion efficiency of Rhodamine B in pure water according to claim 2, characterized in that, The molar ratio of sulfobutyl-β-cyclodextrin added in S1 to rhodamine B added in S2 is: rhodamine B: sulfobutyl-β-cyclodextrin = 1: 0.9-1.

1.

4. The method for improving the energy conversion efficiency of Rhodamine B in pure water according to claim 2, characterized in that, The dissolution temperature in S1 is 20℃-30℃, and the time is 2-3 hours.

5. The method for improving the energy conversion efficiency of Rhodamine B in pure water according to claim 2, characterized in that, The dissolution method in S1 is ultrasonic oscillation or cyclic stirring.

6. The method for improving the energy conversion efficiency of Rhodamine B in pure water according to claim 2, characterized in that, The dissolution temperature of S2 is 20℃-30℃, and the time is 2-3 h.

7. A method for improving the energy conversion efficiency of Rhodamine B in pure water according to claim 2, characterized in that, The dissolution method in S2 is ultrasonic oscillation or cyclic stirring.

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