Method for preparing functional ceanothus goryllus fiber compound feed

Abstract

The application discloses a method for preparing functional caragana fiber compound feed and belongs to the technical field of feed processing. The method comprises the following steps: taking caragana as raw material, performing solid-liquid separation after dilute sulfuric acid pretreatment, preparing cordycepin and lysine through liquid state fermentation of cordyceps militaris and glutamic acid corynebacterium respectively, neutralizing caragana solid fiber and caragana fiber raw material obtained through ammonia treatment, and preparing fiber feed through solid anaerobic fermentation of the compound fermentation strain, and finally, compounding the three to obtain functional caragana fiber compound feed. The method realizes efficient utilization of all components of caragana sugar and fiber resources, and the prepared compound feed is rich in cordycepin, lysine and dietary fiber, the crude protein content of the fiber feed is more than 20%, and the fiber feed is rich in cordycepin and lysine functional components. The method not only provides a new way for resource utilization and high value utilization of caragana, but also provides a new idea for research and development of functional coarse feed, and has good economic, ecological and industrial popularization value.

Description

Technical Field

[0001] This invention belongs to the field of feed processing technology, specifically relating to a method for preparing functional Caragana korshinskii fiber composite feed. Background Technology

[0002] Caragana korshinskii, a deciduous shrub belonging to the genus Caragana in the legume family, is a typical advantageous plant for windbreak, sand fixation, and soil and water conservation in arid and semi-arid regions of northern my country. Its resource reserves are abundant, and the plant is rich in usable components such as cellulose, hemicellulose, and polysaccharides, making it a natural feed ingredient with great development potential. Currently, the feed utilization of Caragana korshinskii mainly focuses on silage, simple crushing and direct addition as roughage, or simple mixing with other feeds. While this can provide a basic supply of roughage and alleviate the shortage of feed ingredients in northern regions, this basic utilization method has significant technical shortcomings: on the one hand, Caragana korshinskii has a high crude fiber content in its original state, resulting in low digestibility and poor palatability for livestock and poultry, leading to a significant reduction in its nutritional conversion rate; on the other hand, the utilization of Caragana korshinskii remains at the level of extensive use of the raw material as a whole, without refined analysis and targeted development of its internal sugars, fiber, and other components, resulting in a waste of Caragana korshinskii resources and failing to achieve full-value utilization, seriously restricting the high-value industrial application of Caragana korshinskii in the feed sector.

[0003] Meanwhile, as the livestock industry develops towards refinement and efficiency, the research and application of functional feeds and feed additives have become the mainstream direction of industry development. Lysine, as an essential limiting amino acid for the growth and development of livestock and poultry, cannot be synthesized by the livestock and poultry bodies themselves and is a core nutrient additive in feed formulations. Its addition amount directly affects the growth performance and breeding efficiency of livestock and poultry. Cordycepin and Cordyceps militaris, with their unique physiological activities, have shown excellent effects in improving the immunity of livestock and poultry, improving the intestinal microecology, and enhancing the production performance and quality of livestock and poultry products. They have become an important direction for the research and development of functional feeds, and the demand for their application in poultry, livestock, and aquaculture continues to grow. However, the traditional preparation processes of cordycepin and lysine currently mostly use pure grain crops such as corn and wheat as the main raw materials. This not only results in high raw material costs but also poses the problem of competing with grain for land, making it difficult to widely apply in the feed industry and limiting the industrialization of functional feeds.

[0004] Furthermore, in the existing feed processing field, there is no technical solution that combines the full utilization of Caragana korshinskii resources with the preparation of functional feed additives. This not only fails to solve the industry pain point of low utilization efficiency of Caragana korshinskii feed, but also makes it difficult to find an effective way to prepare cordycepin and lysine at low cost. As a result, the research and development of functional roughage lacks new raw materials and technical support, and the industry development has reached a bottleneck.

[0005] Based on this, it is necessary to develop a preparation method that enables the full-component, high-value utilization of Caragana korshinskii resources, while simultaneously using Caragana korshinskii as a raw material to prepare functional components such as cordycepin and lysine at low cost, and then scientifically compounding them with Caragana korshinskii fiber feed. This method addresses the technical deficiencies of traditional Caragana korshinskii feed utilization and the cost issues in preparing functional feed additives. It is of great practical significance for promoting the green development of the feed industry, improving the efficiency of livestock farming, and realizing the economic value transformation of Caragana korshinskii ecological resources. It is also a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0006] The technical problem solved by this invention is to provide a method for preparing functional Caragana korshinskii fiber compound feed. This method realizes the full utilization of solid and liquid components of Caragana korshinskii raw materials. Caragana korshinskii hydrolysate is used for fermentation to prepare cordycepin and lysine, and solid fiber is used for fermentation to prepare fiber feed. Finally, a high-nutrition, multifunctional compound feed is obtained by compounding.

[0007] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows:

[0008] A method for preparing functional Caragana korshinskii fiber compound feed includes the following steps:

[0009] 1) Dry, crush, and sieve the lemon twigs, and dry them to constant weight; take lemon twig powder and pre-treat it with dilute sulfuric acid solution to obtain lemon twig acid hydrolysis product; take lemon twig powder and pre-treat it with ammonia solution to obtain lemon twig ammoniated fiber.

[0010] 2) Add ammonia to the acid hydrolysis product of Caragana korshinskii to adjust the pH to neutral, and separate the solid and liquid to obtain Caragana korshinskii hydrolysate and Caragana korshinskii solid fiber; dry the solid fiber for later use, and treat the hydrolysate with activated carbon to remove fermentation inhibitors to obtain purified Caragana korshinskii hydrolysate;

[0011] 3) Cordycepin fermentation broth was prepared by liquid fermentation of Cordyceps militaris using purified Caragana korshinskii hydrolysate as substrate;

[0012] 4) Using purified Ligusticum striatum hydrolysate as a substrate, lysine fermentation broth was prepared by liquid fermentation with Corynebacterium glutamicum;

[0013] 5) Mix acidified and ammonified Caragana korshinskii fiber and then perform solid-state anaerobic fermentation with a compound fermentation strain to obtain fiber protein feed;

[0014] 6) The cordycepin fermentation liquid and lysine fermentation liquid are compounded with fiber protein feed to prepare functional Caragana fiber compound feed.

[0015] Further, in step 1), the acid pretreatment is as follows: add a 1.4% (v / v) dilute sulfuric acid solution at a solid-liquid ratio of 1:10 and pretreat at 128°C for 88 min.

[0016] Further, in step 1), the ammonia pretreatment is as follows: add an ammonia solution with a volume fraction of 4% at a solid-liquid ratio of 1:1, and pretreat at 121℃ for 30 min.

[0017] Further, in step 2), the activated carbon treatment conditions are: add 15% activated carbon, shake at 55°C and 130 rpm, and then filter.

[0018] Furthermore, in step 3), the Cordyceps militaris fermentation uses PDA fermentation medium, with an inoculum of 3% hydrolysate and 7.5% seed liquid, and is cultured aerobically at 25°C for 7 days.

[0019] Further, in step 4), Corynebacterium glutamicum is inoculated onto glucose nutrient agar slant medium for activation. Glucose nutrient agar fermentation medium is used, with hydrolysate added at 35 mL / 50 mL and seed liquid inoculated at 10%. Lysine fermentation broth is obtained by aerobic culture at 28℃ for 2 days.

[0020] Further, in step 5), the compound fermentation strain is activated for 3-5 days at a ratio of strain:water:brown sugar = 1:10:1, the mass ratio of acidified and ammonified Caragana korshinskii fiber is 1:1, the inoculum amount is 10%, the moisture content is 60%, and the fermentation is carried out at 28℃ for 10 days.

[0021] Furthermore, the functional Caragana fiber compound feed has a crude protein content of 20-25% and a neutral detergent fiber content of 55-65%.

[0022] Furthermore, the functional Caragana fiber composite feed prepared by the method described above is a Caragana fiber composite feed.

[0023] Furthermore, the aforementioned functional Caragana fiber compound feed is used as a functional roughage or feed additive for livestock and poultry.

[0024] Compared with the prior art, the present invention has the following advantages:

[0025] (1) This invention realizes the efficient utilization of all components of the raw material of Caragana korshinskii. The Caragana korshinskii hydrolysate and solid fiber after dilute acid pretreatment are fermented in liquid and solid states respectively, without waste of raw materials, greatly improving the feed utilization value of Caragana korshinskii, and providing a new technical route for the resource utilization of Caragana korshinskii.

[0026] (2) The compound feed prepared by the present invention has both nutritional value and functionality. It not only improves the crude protein content of fiber feed and reduces the neutral detergent fiber content through fermentation, thus improving the palatability and digestibility of Caragana feed, but also introduces functional components such as cordycepin and lysine, which makes up for the deficiency of the single nutrition of traditional Caragana feed. Adding it to livestock and poultry feed can improve the physical performance of livestock and poultry.

[0027] (3) This invention uses hydrolysate of Ligusticum striatum to replace traditional pure grain raw materials for fermentation to prepare cordycepin and lysine, which greatly reduces the production cost of functional feed additives and realizes the resource utilization of industrial by-products, which is in line with the green and environmentally friendly industrial development concept.

[0028] (4) The preparation process parameters of the present invention are clear and reproducible. All fermentation conditions have been optimized by single-factor experiments to obtain the best process parameters. The content of fermentation products and feed nutrition indicators are stable and suitable for large-scale industrial production. Attached Figure Description

[0029] Figure 1 This is a roadmap for the preparation method of the functional Caragana fiber compound feed of this application. Detailed Implementation

[0030] The present invention will be further illustrated below with reference to specific embodiments. These embodiments are implemented based on the technical solutions of the present invention, and it should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0031] The Cordyceps militaris used in the following examples was purchased from the China Agricultural Microbial Culture Collection Center (ACCC), number ACCC 50632; Yijiayi straw fermentation agent was purchased from Zhengzhou Yijiayi Biotechnology Co., Ltd.; and Corynebacterium glutamicum was purchased from the China Industrial Microbial Culture Collection Center (CICC), number CICC 20206.

[0032] The HPLC detection conditions for cordycepin in the following examples were as follows: column: Pollshell 120-C18; detector: UV detector (260 nm); column temperature: 30℃; mobile phase: water / methanol = 85:15 (v / v); flow rate: 1 mL / min.

[0033] The HPLC detection conditions for lysine were as follows: column: ChromCore HILIC-Amide (4.6×250 mm); detector: differential refractive index detector (30℃); column temperature: 30℃; mobile phase: acetonitrile / 100 mM ammonium acetate solution = 7:3 (v / v); flow rate: 1 mL / min.

[0034] Crude protein content was determined by the Kjeldahl method, and neutral detergent fiber content was determined according to GB / T 20806-2022 standard.

[0035] Example 1

[0036] A method for preparing functional Caragana korshinskii fiber compound feed includes the following steps:

[0037] (1) Pretreatment of Caragana korshinskii raw materials: Caragana korshinskii purchased from Fengzhen City, Ulanqab was dried at 50℃, pulverized and passed through a 40-mesh sieve, and dried again to constant weight; 50.0g of Caragana korshinskii powder was accurately weighed and added to 500mL of 1.4% (v / v) dilute sulfuric acid solution at a solid-liquid ratio of 1:10, and pretreated at 128℃ for 88min to obtain the acid hydrolysis product of Caragana korshinskii. 40.0g of Caragana korshinskii powder was accurately weighed and added to 4% (v / v) ammonia solution at a solid-liquid ratio of 1:1, and pretreated at 121℃ for 30min to obtain ammoniated Caragana korshinskii fiber.

[0038] (2) Post-treatment of acid hydrolysis products: Ammonia water was added to the acid hydrolysis products of Caragana korshinskii to adjust the pH to 7.0. Solid-liquid separation was performed to obtain Caragana korshinskii hydrolysate and Caragana korshinskii solid fiber. The Caragana korshinskii solid fiber was dried at 65°C for later use. 15% activated carbon was added to the Caragana korshinskii hydrolysate and shaken at 55°C and 130 rpm to remove inhibitors. After filtration, purified Caragana korshinskii hydrolysate was obtained.

[0039] (3) Preparation of cordycepin by liquid fermentation of Ligusticum striatum hydrolysate:

[0040] ① Inoculate Cordyceps militaris onto PDA slant medium and culture at 25℃ for 7 days to obtain second-generation strains. Transfer to PDA plate medium and culture at 25℃ until the mycelium fully colonizes the plate.

[0041] ② Take 3 pieces of 6mm mycelium cake and inoculate them into 50mL of PDA seed culture medium. Incubate at 25℃ and 200r / min for 7 days to obtain the initial seed culture of Cordyceps militaris.

[0042] ③ Prepare PDA fermentation medium (12g / L potato extract powder, purified Ligusticum striatum hydrolysate), inoculate with 3% hydrolysate and 7.5% seed liquid, fill with 50mL / 200mL of liquid, and culture at 25℃ for 7 days to obtain cordycepin fermentation broth. The cordycepin content was determined by HPLC and found to be 0.065g / L.

[0043] (4) Solid-state fermentation of Caragana korshinskii fiber to prepare fiber feed:

[0044] ① Mix Yijiayi compound fermentation bacteria, water, and brown sugar in a ratio of 1:10:1, seal and ferment for 4 days to activate the liquid bacteria;

[0045] ② The dried acidified Caragana korshinskii fiber and ammoniated Caragana korshinskii fiber were mixed evenly in a 1:1 mass ratio. The mixed Caragana korshinskii solid fiber was spread evenly on the fermentation bottle. Activated bacteria were sprinkled on each layer. The inoculum content was controlled at 10% and the material moisture content at 60%. After compaction, the bottle was sealed with nitrogen gas and anaerobic fermented at 28℃ for 10 days to obtain fiber protein feed. The crude protein content was 17.89% and the neutral detergent fiber content was 73.14%.

[0046] (5) Preparation of functional Caragana fiber compound feed: Based on 100.0g of fiber protein feed, add 10% of cordycepin fermentation liquid by mass, mix the two thoroughly, dry at 65℃ for 12h, and granulate to obtain functional Caragana fiber compound feed.

[0047] Testing revealed that the compound feed prepared in this embodiment contains 20.35% crude protein, 0.78g cordycepin equivalent per 50g of Caragana korshinskii, and 63.33% neutral detergent fiber. It has good palatability and can be used as a functional roughage or feed additive for livestock and poultry.

[0048] Example 2

[0049] A method for preparing functional Caragana korshinskii fiber compound feed includes the following steps:

[0050] (1) Pretreatment of Caragana korshinskii raw materials: Caragana korshinskii purchased from Fengzhen City, Ulanqab was dried at 50℃, pulverized and passed through a 40-mesh sieve, and dried again to constant weight; 50.0g of Caragana korshinskii powder was accurately weighed and added to 500mL of 1.4% (v / v) dilute sulfuric acid solution at a solid-liquid ratio of 1:10, and pretreated at 128℃ for 88min to obtain the acid hydrolysis product of Caragana korshinskii. 40.0g of Caragana korshinskii powder was accurately weighed and added to 4% (v / v) ammonia solution at a solid-liquid ratio of 1:1, and pretreated at 121℃ for 30min to obtain ammoniated Caragana korshinskii fiber.

[0051] (2) Post-treatment of acid hydrolysis products: Ammonia water was added to the acid hydrolysis products of Caragana korshinskii to adjust the pH to 7.0. Solid-liquid separation was performed to obtain Caragana korshinskii hydrolysate and Caragana korshinskii solid fiber. The Caragana korshinskii solid fiber was dried at 65°C for later use. 15% activated carbon was added to the Caragana korshinskii hydrolysate and shaken at 55°C and 130 rpm to remove inhibitors. After filtration, purified Caragana korshinskii hydrolysate was obtained.

[0052] (3) Preparation of lysine by liquid fermentation of Ligusticum striatum hydrolysate:

[0053] ① Inoculate Corynebacterium glutamicum onto glucose nutrient agar slant medium and culture at 28℃ for 2 days to obtain second generation strains. Transfer to plate medium and culture at 28℃ for 1 day.

[0054] ② Take 3 pieces of 6mm mycelial cake and inoculate them into 50mL of glucose nutrient agar seed culture medium. Incubate at 28℃ and 180r / min for 2 days to obtain the initial seed culture of Corynebacterium glutamicum.

[0055] ③ Prepare glucose nutrient agar fermentation medium (30 g / L peptone, 5 g / L beef extract, purified Ligusticum striatum hydrolysate, 5 g / L sodium chloride), inoculate with 35 mL / 50 mL hydrolysate and 10% seed liquid, fill with 50 mL / 200 mL of liquid, and culture at 28℃ for 2 days to obtain lysine fermentation broth. The lysine content was determined by HPLC and found to be 16.781 g / L.

[0056] (4) Solid-state fermentation of Caragana korshinskii fiber to prepare fiber feed:

[0057] ① Mix Yijiayi compound fermentation bacteria, water, and brown sugar in a ratio of 1:10:1, seal and ferment for 4 days to activate the liquid bacteria;

[0058] ② The dried acidified Caragana korshinskii fiber and ammoniated Caragana korshinskii fiber were mixed evenly in a 1:1 mass ratio. The mixed Caragana korshinskii solid fiber was spread evenly on the fermentation bottle. Activated bacteria were sprinkled on each layer. The inoculum content was controlled at 10% and the material moisture content was controlled at 60%. After compaction, nitrogen gas was filled and sealed. Anaerobic fermentation was carried out at 28℃ for 10 days to obtain fiber feed. The crude protein content was 17.89% and the neutral detergent fiber content was 73.14%.

[0059] (5) Preparation of functional Caragana fiber compound feed: Based on 100.0g of fiber protein feed, add 15% lysine fermentation liquid by mass, mix the two thoroughly, dry at 65℃ for 12h, and after granulation, obtain functional Caragana fiber compound feed.

[0060] Testing revealed that the compound feed prepared in this embodiment contains 20.57% crude protein, 8.47g of lysine (50g of Caragana korshinskii equivalent), and 63.69% neutral detergent fiber. It exhibits good palatability and can be used as a functional roughage or feed additive for livestock and poultry.

[0061] Example 3

[0062] like Figure 1 As shown in the figure, this embodiment provides a method for preparing functional Caragana korshinskii fiber compound feed, including the following steps:

[0063] (1) Pretreatment of Caragana korshinskii raw materials: Caragana korshinskii purchased from Fengzhen City, Ulanqab was dried at 50℃, pulverized and passed through a 40-mesh sieve, and dried again to constant weight; 50.0g of Caragana korshinskii powder was accurately weighed and added to 500mL of 1.4% (v / v) dilute sulfuric acid solution at a solid-liquid ratio of 1:10, and pretreated at 128℃ for 88min to obtain the acid hydrolysis product of Caragana korshinskii. 40.0g of Caragana korshinskii powder was accurately weighed and added to 4% (v / v) ammonia solution at a solid-liquid ratio of 1:1, and pretreated at 121℃ for 30min to obtain ammoniated Caragana korshinskii fiber.

[0064] (2) Post-treatment of acid hydrolysis products: Ammonia water was added to the acid hydrolysis products of Caragana korshinskii to adjust the pH to 7.0. Solid-liquid separation was performed to obtain Caragana korshinskii hydrolysate and Caragana korshinskii solid fiber. The Caragana korshinskii solid fiber was dried at 65°C for later use. 15% activated carbon was added to the Caragana korshinskii hydrolysate and shaken at 55°C and 130 rpm to remove inhibitors. After filtration, purified Caragana korshinskii hydrolysate was obtained.

[0065] (3) Preparation of cordycepin by liquid fermentation of Ligusticum striatum hydrolysate:

[0066] ① Inoculate Cordyceps militaris onto PDA slant medium and culture at 25℃ for 7 days to obtain second-generation strains. Transfer to PDA plate medium and culture at 25℃ until the mycelium fully colonizes the plate.

[0067] ② Take 3 pieces of 6mm mycelium cake and inoculate them into 50mL of PDA seed culture medium. Incubate at 25℃ and 200r / min for 7 days to obtain the initial seed culture of Cordyceps militaris.

[0068] ③ Prepare PDA fermentation medium (12g / L potato extract powder, purified Ligusticum striatum hydrolysate), inoculate with 3% hydrolysate and 7.5% seed liquid, fill with 50mL / 200mL of liquid, and culture at 25℃ for 7 days to obtain cordycepin fermentation broth. The cordycepin content was determined by HPLC and found to be 0.065g / L.

[0069] (4) Preparation of lysine by liquid fermentation of Ligusticum striatum hydrolysate:

[0070] ① Inoculate Corynebacterium glutamicum onto glucose nutrient agar slant medium and culture at 28℃ for 2 days to obtain second generation strains. Transfer to plate medium and culture at 28℃ for 1 day.

[0071] ② Take 3 pieces of 6mm mycelial cake and inoculate them into 50mL of glucose nutrient agar seed culture medium. Incubate at 28℃ and 180r / min for 2 days to obtain the initial seed culture of Corynebacterium glutamicum.

[0072] ③ Prepare glucose nutrient agar fermentation medium (30 g / L peptone, 5 g / L beef extract, purified Ligusticum striatum hydrolysate, 5 g / L sodium chloride), inoculate with 35 mL / 50 mL hydrolysate and 10% seed liquid, fill with 50 mL / 200 mL of liquid, and culture at 28℃ for 2 days to obtain lysine fermentation broth. The lysine content was determined by HPLC and found to be 16.78 g / L.

[0073] (5) Solid-state fermentation of Caragana korshinskii fiber to prepare fiber feed:

[0074] ① Mix Yijiayi compound fermentation bacteria, water, and brown sugar in a ratio of 1:10:1, seal and ferment for 4 days to activate the liquid bacteria;

[0075] ② The dried acidified Caragana korshinskii fiber and ammoniated Caragana korshinskii fiber were mixed evenly in a 1:1 mass ratio. The mixed Caragana korshinskii solid fiber was spread evenly on the fermentation bottle. Activated bacteria were sprinkled on each layer. The inoculum content was controlled at 10% and the material moisture content was controlled at 60%. After compaction, nitrogen gas was filled and sealed. Anaerobic fermentation was carried out at 28℃ for 10 days to obtain fiber feed. The crude protein content was 17.89% and the neutral detergent fiber content was 73.14%.

[0076] (6) Preparation of functional Caragana fiber compound feed: Based on 100.0g of fiber protein feed, add 10% by mass of cordycepin fermentation liquid and 15% by mass of lysine fermentation liquid, mix the three thoroughly, dry at 65℃ for 12h, and granulate to obtain functional Caragana fiber compound feed.

[0077] Testing revealed that the compound feed prepared in this embodiment contains 23.17% crude protein, 0.78g cordycepin (50g equivalent of Caragana korshinskii), 8.63g lysine (50g equivalent of Caragana korshinskii), and 57.74% neutral detergent fiber. It has good palatability and can be used as a functional roughage or feed additive for livestock and poultry.

[0078] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for preparing functional Caragana korshinskii fiber compound feed, characterized in that: Includes the following steps: 1) Dry, crush, and sieve the lemon twigs, and dry them to constant weight; take lemon twig powder and pre-treat it with dilute sulfuric acid solution to obtain lemon twig acid hydrolysis product; take lemon twig powder and pre-treat it with ammonia solution to obtain lemon twig ammoniated fiber. 2) Add ammonia to the acid hydrolysis product of Caragana korshinskii to adjust the pH to neutral, and separate the solid and liquid to obtain Caragana korshinskii hydrolysate and Caragana korshinskii solid fiber; dry the solid fiber for later use, and treat the hydrolysate with activated carbon to remove fermentation inhibitors to obtain purified Caragana korshinskii hydrolysate; 3) Cordycepin fermentation broth was prepared by liquid fermentation of Cordyceps militaris using purified Caragana korshinskii hydrolysate as substrate; 4) Using purified Ligusticum striatum hydrolysate as a substrate, lysine fermentation broth was prepared by liquid fermentation with Corynebacterium glutamicum; 5) Mix acidified and ammonified Caragana korshinskii fiber and then perform solid-state anaerobic fermentation with a compound fermentation strain to obtain fiber protein feed; 6) The cordycepin fermentation liquid and lysine fermentation liquid are compounded with fiber protein feed to prepare functional Caragana fiber compound feed.

2. The method for preparing functional Caragana fiber composite feed according to claim 1, characterized in that: In step 1), the acid pretreatment is as follows: add a 1.4% (v / v) dilute sulfuric acid solution at a solid-liquid ratio of 1:10 and pretreat at 128°C for 88 min.

3. The method for preparing functional Caragana fiber compound feed according to claim 1, characterized in that: In step 1), the ammonia pretreatment is as follows: add 4% ammonia solution at a solid-liquid ratio of 1:1 and pretreat at 121℃ for 30 min.

4. The method for preparing functional Caragana fiber compound feed according to claim 1, characterized in that: In step 2), the activated carbon treatment conditions are as follows: add 15% activated carbon, shake at 55°C and 130 rpm, and then filter.

5. The method for preparing functional Caragana fiber compound feed according to claim 1, characterized in that: In step 3), Cordyceps militaris fermentation was carried out using PDA fermentation medium, with 3% hydrolysate inoculation and 7.5% seed liquid inoculation, and aerobic culture at 25℃ for 7 days.

6. The method for preparing functional Caragana fiber compound feed according to claim 1, characterized in that: In step 4), Corynebacterium glutamicum was inoculated onto glucose nutrient agar slant medium for activation. Glucose nutrient agar fermentation medium was used, with hydrolysate added at 35 mL / 50 mL and seed liquid inoculated at 10%. Lysine fermentation broth was obtained by aerobic culture at 28℃ for 2 days.

7. The method for preparing functional Caragana fiber compound feed according to claim 1, characterized in that: In step 5), the compound fermentation strain is activated for 3-5 days at a ratio of strain:water:brown sugar = 1:10:1, the mass ratio of acidified and ammonified Caragana korshinskii fiber is 1:1, the inoculum amount is 10%, the moisture content is 60%, and the fermentation is carried out at 28℃ for 10 days.

8. The method for preparing functional Caragana fiber compound feed according to claim 1, characterized in that: The functional Caragana fiber compound feed has a crude protein content of 20-25% and a neutral detergent fiber content of 55-65%.

9. The functional Caragana fiber compound feed prepared by the method for preparing functional Caragana fiber compound feed according to any one of claims 1 to 8.

10. The use of the functional Caragana fiber compound feed according to claim 9 as a functional roughage or feed additive for livestock and poultry.

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