A PDX model tumor tissue continuous implantation device

Abstract

This invention provides a continuous implantation device for PDX model tumor tissue, comprising: a main cannula; and a pushing component disposed at one end of the main cannula. The continuous implantation device for PDX model tumor tissue provided by this invention utilizes the cooperation of structures such as the main cannula, the pushing component, the tissue sampler, and the retaining spring. The tissue sampler can be pre-loaded with multiple tumor tissue blocks, the retaining spring enables precise positioning and switching of the tissue sampler, the pushing component can sequentially push the tissue blocks for implantation, and the main cannula can be externally connected to an syringe to achieve simultaneous delivery of gel and tissue blocks. This device enables continuous implantation of tumor tissue blocks without the need for repeated clamping and transfer operations, significantly improving implantation efficiency and avoiding the decrease in activity caused by prolonged exposure of tissue blocks outside the body. Furthermore, it eliminates the need for clamping tissue blocks during transfer, reducing the risk of mechanical damage and ensuring the integrity of the tissue blocks. It also enables simultaneous delivery of tissue blocks, gel, and drugs, improving the experimental adaptability of the device.

Description

Technical Field

[0001] This invention relates to the field of animal model construction technology, and in particular to a PDX model tumor tissue continuous implantation device. Background Technology

[0002] The PDX (patient-derived xenograft) model, a core tool in precision medicine and cancer research, directly implants fresh tumor tissue from patients into immunodeficient mice, preserving the histological characteristics, molecular heterogeneity, and drug responsiveness of the primary tumor. It plays an irreplaceable role in areas such as anticancer drug screening, drug resistance mechanism research, and personalized treatment plan evaluation. A key step in constructing this model is cutting fresh tumor tissue obtained from surgery or biopsy into 1-3 mm³ blocks and precisely and rapidly implanting them subcutaneously into experimental animals under sterile conditions to maximize tumor tissue viability and ensure a high success rate in model construction.

[0003] Currently, laboratories generally use traditional manual implantation tools and methods to complete this crucial step, relying heavily on a combination of surgical instruments (such as tissue forceps, cannulas, and ordinary syringes). Essentially, it's a simplified open surgical procedure requiring step-by-step implantation of the tissue block through "tissue cutting - grasping and transferring - wound dissection - pushing and placing." However, these traditional methods are limited by the design of the tools, resulting in numerous insurmountable technical defects that severely restrict the efficiency and consistency of PDX model construction.

[0004] First, the operation process is cumbersome and inefficient, making continuous implantation impossible. Existing tools are all designed as "single loading - single implantation," lacking continuous material supply and integrated operation mechanisms. After each tissue block implantation, repeated actions such as instrument exchange, tissue block retrieval, and wound positioning are required, resulting in a lengthy and inconsistent operation chain. For the needs of large-scale, high-throughput PDX model construction, this method is time-consuming and labor-intensive, not only severely reducing experimental efficiency but also causing tumor tissue to be exposed to the in vitro environment for too long due to excessive operation time, leading to decreased activity and thus reducing the model success rate.

[0005] Secondly, the risk of tissue block damage is high, and the consistency of model quality is poor. Traditional methods rely on tissue forceps to hold tissue blocks for transfer. The holding force depends entirely on the operator's experience, which can easily cause mechanical damage to the tissue blocks due to excessive force, thus destroying the integrity of tumor cells. At the same time, during manual pushing, tissue blocks are prone to falling, shifting, or adhering to the instrument surface, resulting in implantation position deviation and damage to the integrity of the tissue blocks. Ultimately, this leads to model differences between different experimental individuals, affecting the reliability of research data.

[0006] Third, the technical threshold is high and the margin for error is low. The entire implantation process is highly dependent on the operator's hand stability and skill level. From picking up the tissue block and transferring it to the precise insertion into the target position, each step requires meticulous control. Novices need long-term training to master it. Moreover, manual operation is easily affected by factors such as fatigue and environmental interference, which can lead to operational errors and further increase the experimental and time costs.

[0007] Fourth, it lacks standardized auxiliary functions and has insufficient adaptability. Traditional tools lack precise push distance control and positioning structures, which cannot guarantee the consistency of tissue block implantation depth; at the same time, they cannot achieve synchronous delivery of tumor tissue blocks and auxiliary materials such as biogels and drugs, making it difficult to meet the advanced experimental needs of PDX-based combination therapy research and tissue survival optimization.

[0008] Therefore, it is necessary to provide a PDX model tumor tissue continuous implantation device to solve the above-mentioned technical problems. Summary of the Invention

[0009] This invention provides a continuous implantation device for PDX model tumor tissue, which solves the problems of existing implantation tools being unable to achieve continuous implantation, having a high risk of tissue block damage, being cumbersome to operate, and having insufficient adaptability.

[0010] To address the aforementioned technical problems, this invention provides a continuous implantation device for PDX model tumor tissue, comprising: Main bushing; A pushing component is disposed at one end of the main sleeve; A tissue sampler, wherein the tissue sampler is disposed inside the main sleeve; A retaining ring is disposed on the side of the tissue sampler and located inside the main sleeve.

[0011] Preferably, the main sleeve includes a tube body, the inside of which is provided with a tissue delivery chamber, a tissue propulsion tube is installed on the side of the tube body, the inside of which is provided with a tissue propulsion chamber, and a connecting tube is installed on the side of which.

[0012] Preferably, the pushing assembly includes a pushing rod, one end of which is disposed inside the tissue pushing cavity, and a piston is fixedly installed at one end of the pushing rod, the piston being slidably connected to the inner side of the tissue pushing cavity.

[0013] Preferably, the tissue sampler includes a column, which is disposed inside the tissue sample delivery chamber. The side of the column is provided with a retaining spring groove, and the top of the column is provided with a plurality of tissue compartments evenly distributed inside.

[0014] Preferably, the snap ring is engaged with the inner side of the snap ring groove.

[0015] Preferably, the push rod has a graduated groove on its side and a limiting component is fitted on its outer side.

[0016] Preferably, the limiting component includes a limiting ring, a through groove is provided on one side of the limiting ring, a rotating seat is fixedly installed on one side of the limiting ring, a rotating shaft is fixedly installed inside the rotating seat, a pressing plate is rotatably connected to the outer side of the rotating shaft, and a snap-fit ​​block is fixedly installed at one end of the pressing plate.

[0017] Preferably, the snap-fit ​​block snaps onto the inner side of the scale groove.

[0018] Preferably, a torsion spring is fixedly installed on the outer side of the rotating shaft, and one end of the torsion spring is fixedly installed on the side of the pressing plate.

[0019] To address the above problems, the present invention also provides a method of using the PDX model tumor tissue continuous implantation device described in any of the above claims, comprising the following steps: S1. Device Assembly and Pre-assembly: Assemble the main sleeve, push assembly, tissue sampler, and retaining ring. Seal the syringe containing biogel or liquid drug to the connecting tube of the main sleeve. Under sterile conditions, place the prepared 1-3 mm³ tumor tissue blocks into each tissue compartment of the tissue sampler in sequence. Push the loaded tissue sampler into the tissue delivery chamber of the main sleeve until the retaining ring engages with the retaining ring groove of the tissue sampler, so that the first tissue compartment is precisely aligned with the tissue push chamber, and the device pre-assembly is completed. S2. Pushing tumor tissue: Hold the main sleeve, pull the push rod of the push component backward to the rear stop point, and then push the push rod forward at a constant speed. The piston at the end of the push rod will push the tumor tissue block in the tissue chamber to the outlet end of the connecting tube and the tissue propulsion chamber. S3. Inject and mix the gel: Keep the push rod in place and push the externally connected syringe plunger to inject a predetermined volume of biological gel or liquid drug into the tissue propulsion cavity, so that the gel immediately encapsulates the tumor tissue block in the cavity to form a tissue-gel complex. S4. Complete a single implantation: Insert the outer cannula needle at the front end of the main cannula into the predetermined position under the skin of the experimental animal, continue to push the push rod forward to the front stop point, push the tissue-gel complex out through the outer cannula needle and inoculate it under the skin of the experimental animal, keep the push rod in place, withdraw the outer cannula needle from the experimental animal, and complete a single implantation. S5. Switch to the next tissue compartment: Pull the push rod back to the rear stop point, push the tissue sampler forward with your finger to make it slide one step, and the retaining spring will snap into another retaining spring from the current retaining slot, so as to achieve precise alignment between the next tissue compartment and the tissue delivery chamber (the bottom of the inner side of the tissue delivery chamber is provided with a retaining slot for engaging with the end face of the retaining spring). S6. Continuous implantation cycle and end: Repeat steps S2-S5 to sequentially implant the tumor tissue blocks in the tissue sampler until all tissue blocks in all tissue compartments of the tissue sampler have been implanted, thus completing the entire implantation process. Disassemble the device and sterilize it.

[0020] Compared with related technologies, the PDX model tumor tissue continuous implantation device provided by the present invention has the following beneficial effects: This invention provides a continuous implantation device for PDX model tumor tissue. The device utilizes a main cannula, a pushing component, a tissue sampler, and a retaining spring to work together. The tissue sampler can be pre-loaded with multiple tumor tissue blocks, the retaining spring enables precise positioning and switching of the tissue sampler, the pushing component sequentially pushes the tissue blocks for implantation, and the main cannula can be connected to an external syringe for simultaneous delivery of gel and tissue blocks. This device enables continuous implantation of tumor tissue blocks without the need for repeated clamping and transfer operations, significantly improving implantation efficiency and avoiding the decrease in activity caused by prolonged exposure of tissue blocks outside the body. Furthermore, it eliminates the need for clamping tissue blocks during transfer, reducing the risk of mechanical damage and ensuring the integrity of the tissue blocks. It also enables simultaneous delivery of tissue blocks, gel, and drugs, improving the experimental adaptability of the device and solving the problems of existing implantation tools, such as inability to achieve continuous implantation, high risk of tissue block damage, cumbersome operation, and insufficient adaptability. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of a first embodiment of a PDX model tumor tissue continuous implantation device provided by the present invention; Figure 2 for Figure 1 The diagram shows a cross-sectional structure. Figure 3 for Figure 2 The diagram shows the structure of the tissue sampler. Figure 4 for Figure 3 The diagram shows the side structure. Figure 5 This is a schematic diagram of the structure of a second embodiment of a PDX model tumor tissue continuous implantation device provided by the present invention; Figure 6 for Figure 5 The enlarged schematic diagram of part A is shown.

[0022] The following are the labeling elements in the diagram: 1. Main sleeve, 11. Tube body, 12. Tissue sample delivery chamber, 13. Tissue propulsion tube, 14. Connecting tube, 2. Pushing assembly, 21. Push rod, 22. Piston, 3. Tissue sample feeder, 31. Column, 32. Snap ring groove, 33. Tissue chamber, 4. Snap ring, 5. Limiting assembly, 51. Limiting ring, 52. Through groove, 53. Rotating seat, 54. Rotating shaft, 55. Pressing plate, 56. Torsion spring, 57. Snap-fit ​​block, 6. Scale groove. Detailed Implementation

[0023] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0024] First Embodiment Please refer to the following: Figure 1 , Figure 2 , Figure 3 , Figure 4 ,in, Figure 1 This is a schematic diagram of the structure of a first embodiment of a PDX model tumor tissue continuous implantation device provided by the present invention; Figure 2 for Figure 1 The diagram shows a cross-sectional structure. Figure 3 for Figure 2 The diagram shows the structure of the tissue sampler. Figure 4 for Figure 3 The diagram shows the side structure.

[0025] A PDX model tumor tissue continuous implantation device includes: a main cannula 1; A pushing component 2 is disposed at one end of the main sleeve 1; Tissue sampler 3, wherein the tissue sampler 3 is disposed inside the main sleeve 1; A retaining ring 4 is disposed on the side of the tissue sampler 3 and located inside the main sleeve 1.

[0026] The main sleeve 1 includes a tube body 11, the inside of which is provided a tissue delivery chamber 12. A tissue delivery tube 13 is installed on the side of the tube body 11, the inside of which is provided a tissue delivery chamber, and a connecting tube 14 is installed on the side of the tissue delivery tube 13.

[0027] The pushing assembly 2 includes a pushing rod 21, one end of which is disposed inside the tissue pushing cavity, and a piston 22 is fixedly installed on one end of the pushing rod 21. The piston 22 is slidably connected to the inner side of the tissue pushing cavity.

[0028] The tissue sampler 3 includes a column 31, which is disposed inside the tissue sample delivery chamber 12. A retaining spring groove 32 is provided on the side of the column 31, and multiple tissue compartments 33 are evenly provided inside the top of the column 31.

[0029] The retaining ring 4 is engaged with the inner side of the retaining ring groove 32.

[0030] The tube body 11 is made of medical-grade stainless steel, with a length of 15-20cm and a diameter of 2-3cm. It has a stable structure and is resistant to high-pressure sterilization. The tissue sample delivery chamber 12 is a cylindrical cavity with a diameter that matches the cylinder 31 of the tissue sampler 3, ensuring smooth sliding. The tissue push tube 13 is integrally formed with the tube body 11, and the diameter of the tissue push chamber matches the piston 22, ensuring sealed delivery. The Luer connector of the connecting tube 14 can be sealed and connected to a standard syringe to achieve stable injection of biogels or liquid drugs. The push rod 21 is made of stainless steel, is 20-25cm long, and has a smooth surface for easy operation; the piston 22 is made of medical rubber, which fits tightly against the inner wall of the tissue propulsion chamber, providing good sealing performance and effectively pushing tissue blocks and tissue-gel complexes to prevent leakage. The column 31 is made of medical-grade plastic, with a length of 10-12cm and a diameter slightly smaller than the tissue sample delivery cavity 12, resulting in low sliding resistance. The tissue chamber 33 is a hemispherical groove with a diameter of 1.5-3.5mm and a depth of 1-2mm, which can stably accommodate tumor tissue blocks of 1-3mm³, preventing displacement during delivery. The retaining ring 4 is a U-shaped elastic steel sheet, which is installed on the side of the column 31. When in use, it is adapted to the retaining groove opened at the bottom of the inner side of the tissue sample delivery cavity 12. There are six retaining grooves to realize the positioning of the tissue sample delivery device 3. When switching, applying a pushing force can make the retaining ring 4 disengage from the current retaining groove, slide to the next retaining groove and engage. The operation is convenient and the positioning is accurate.

[0031] The working principle of the PDX model tumor tissue continuous implantation device provided by the present invention is as follows: Device assembly and pre-assembly: Assemble the main sleeve 1, the push assembly 2, the tissue sampler 3, and the retaining ring 4. Seal the syringe containing the biogel or liquid drug to the connecting tube 14 of the main sleeve 1. Under sterile conditions, place the prepared 1-3 mm³ tumor tissue blocks into the tissue compartments 33 of the tissue sampler 3 in sequence. Push the loaded tissue sampler 3 into the tissue delivery chamber 12 of the main sleeve 1 until the retaining ring 4 engages with the retaining ring groove 32 of the tissue sampler 3, so that the first tissue compartment 33 is precisely aligned with the tissue push chamber, thus completing the device pre-assembly. Pushing tumor tissue: Hold the main sleeve 1, pull the push rod 21 of the push assembly 2 backward to the rear stop point, and then push the push rod 21 forward at a constant speed. The tumor tissue block in the tissue chamber 33 is pushed to the connecting tube 14 and the tissue propulsion cavity through the piston 22 at the end of the push rod 21. Injecting and mixing the gel: Keeping the push rod 21 in place, push the externally connected syringe plunger to inject a predetermined volume of biological gel or liquid drug into the tissue propulsion cavity, so that the gel immediately encapsulates the tumor tissue block in the cavity to form a tissue-gel complex; To complete a single implantation: Insert the outer cannula needle at the front end of the main cannula 1 into the predetermined subcutaneous position of the experimental animal, continue to push the push rod 21 forward to the front stop point, push the tissue-gel complex through the outer cannula needle and inoculate it into the subcutaneous tissue of the experimental animal, keep the push rod 21 in place and withdraw the outer cannula needle from the experimental animal to complete a single implantation; Switch to the next tissue compartment: pull the push rod 21 back to the rear stop point, push the tissue sampler 3 forward with your finger to make it slide one step, and the retaining spring 4 will snap into another retaining spring from the current retaining spring, so as to achieve precise alignment between the next tissue compartment 33 and the tissue delivery cavity (the bottom of the inner side of the tissue sample delivery cavity 12 is provided with a retaining spring for engaging with the end face of the retaining spring 4). Continuous implantation cycle and termination: The tumor tissue blocks in the tissue sampler 3 are continuously implanted in sequence until all tumor tissue blocks in all tissue compartments 33 of the tissue sampler 3 are inoculated, the entire implantation process is completed, the device is disassembled and sterilized.

[0032] Compared with related technologies, the PDX model tumor tissue continuous implantation device provided by the present invention has the following beneficial effects: The device utilizes a combination of components including the main sleeve 1, the push assembly 2, the tissue sampler 3, and the retaining spring 4. The tissue sampler 3 can be pre-loaded with multiple tumor tissue blocks, the retaining spring 4 enables precise positioning and switching of the tissue sampler 3, the push assembly 2 pushes the tissue blocks sequentially for implantation, and the main sleeve 1 can be connected to an external syringe to deliver gel and tissue blocks simultaneously. This allows for continuous implantation of tumor tissue blocks without repeated clamping and transfer operations, significantly improving implantation efficiency and avoiding decreased activity due to prolonged exposure of tissue blocks outside the body. Furthermore, it eliminates the need for clamping tissue blocks during transfer, reducing the risk of mechanical damage and ensuring their integrity. It also enables simultaneous delivery of tissue blocks, gel, and drugs, improving the experimental adaptability of the device and addressing the problems of existing implantation tools, such as inability to achieve continuous implantation, high risk of tissue block damage, cumbersome operation, and insufficient adaptability.

[0033] Second Embodiment Please refer to the following: Figure 5 and Figure 6Based on the continuous implantation device for PDX model tumor tissue provided in the first embodiment of this application, the second embodiment of this application proposes another continuous implantation device for PDX model tumor tissue. The second embodiment is merely a preferred embodiment of the first embodiment, and the implementation of the second embodiment will not affect the separate implementation of the first embodiment.

[0034] Specifically, the second embodiment of this application provides a PDX model tumor tissue continuous implantation device in that the push rod 21 has a scale groove 6 on its side and a limiting component 5 is sleeved on the outer side of the push rod 21.

[0035] The limiting component 5 includes a limiting ring 51, a through groove 52 is provided on one side of the limiting ring 51, a rotating seat 53 is fixedly installed on one side of the limiting ring 51, a rotating shaft 54 ​​is fixedly installed inside the rotating seat 53, a pressing plate 55 is rotatably connected to the outer side of the rotating shaft 54, and a snap-fit ​​block 57 is fixedly installed at one end of the pressing plate 55.

[0036] The snap-fit ​​block 57 snaps into the inner side of the scale groove 6.

[0037] A torsion spring 56 is fixedly installed on the outer side of the rotating shaft 54, and one end of the torsion spring 56 is fixedly installed on the side of the pressing plate 55.

[0038] The graduated grooves 6 are distributed along the length of the push rod 21, with each graduation representing a 0.5mm pushing distance, facilitating precise control of the implantation depth. The limiting ring 51 is an annular structure with an inner diameter slightly larger than the outer diameter of the push rod 21, allowing it to slide freely. When the pressing plate 55 is pressed, it compresses the torsion spring 56, causing the locking block 57 to disengage from the graduated grooves 6, thus adjusting the position of the limiting ring 51. After being released, the torsion spring 56 rebounds, and the locking block 57 embeds into the graduated grooves 6, locking the limiting ring 51 and ensuring a precise and consistent pushing distance.

[0039] The working principle of the PDX model tumor tissue continuous implantation device provided by the present invention is as follows: When in use, press the pressing plate 55 according to the preset implantation depth, adjust the limiting ring 51 to the corresponding scale groove 6 position, and release the pressing plate 55 to complete the locking; when pushing the tissue block and the composite, push the pushing rod 21 until the limiting ring 51 abuts against the end face of the tissue pushing tube 13 to stop pushing, ensuring that the implantation depth is consistent each time; if the implantation depth needs to be adjusted, repeat the above steps to reset the position of the limiting ring 51.

[0040] Compared with related technologies, the PDX model tumor tissue continuous implantation device provided by the present invention has the following beneficial effects: The limiting assembly 5 consists of a limiting ring 51, a rotating seat 53, a rotating shaft 54, a pressing plate 55, a torsion spring 56, a locking block 57, and a graduated groove 6. These components work together to allow the locking block 57 to disengage from the graduated groove 6 when the pressing plate 55 is pressed. After the limiting ring 51 slides to the corresponding graduation, it is released, and the torsion spring 56 resets, allowing the locking block 57 to engage with the graduated groove 6 and complete the locking process. This allows for precise control of the pushing distance of the push rod 21, ensuring consistent implantation depth of the tumor tissue block each time and avoiding implantation position deviations caused by manual operation.

[0041] To address the above problems, the present invention also provides a method of using the PDX model tumor tissue continuous implantation device described in any of the above claims, comprising the following steps: S1. Device Assembly and Pre-assembly: Assemble the main sleeve 1, the push assembly 2, the tissue sampler 3, and the retaining ring 4. Seal the syringe containing the biogel or liquid drug to the connecting tube 14 of the main sleeve 1. Under sterile conditions, place the prepared 1-3 mm³ tumor tissue blocks into each tissue compartment 33 of the tissue sampler 3 in sequence. Push the loaded tissue sampler 3 into the tissue delivery chamber 12 of the main sleeve 1 until the retaining ring 4 engages with the retaining ring groove 32 of the tissue sampler 3, so that the first tissue compartment 33 is precisely aligned with the tissue push chamber, thus completing the device pre-assembly. S2. Pushing tumor tissue: Hold the main sleeve 1, pull the push rod 21 of the push assembly 2 backward to the rear stop point, and then push the push rod 21 forward at a constant speed. The tumor tissue block in the tissue chamber 33 is pushed to the connecting tube 14 and the tissue propulsion cavity through the piston 22 at the end of the push rod 21. S3. Inject and mix the gel: Keep the push rod 21 in place and push the externally connected syringe plunger to inject a predetermined volume of biological gel or liquid drug into the tissue propulsion cavity, so that the gel immediately encapsulates the tumor tissue block in the cavity to form a tissue-gel complex. S4. Complete a single implantation: Insert the outer cannula needle at the front end of the main cannula 1 into the predetermined position under the skin of the experimental animal, continue to push the push rod 21 forward to the front stop point, push the tissue-gel complex through the outer cannula needle and inoculate it under the skin of the experimental animal, keep the push rod 21 in place, withdraw the outer cannula needle from the experimental animal, and complete a single implantation. S5. Switch to the next tissue compartment: Pull the push rod 21 back to the rear stop point, push the tissue sampler 3 forward with your finger to make it slide one step, and the retaining spring 4 will be inserted from the current retaining slot into another retaining slot to achieve precise alignment between the next tissue compartment 33 and the tissue delivery cavity (the bottom of the inner side of the tissue sample delivery cavity 12 is provided with a retaining slot for engaging with the end face of the retaining spring 4). S6. Continuous implantation cycle and end: Repeat steps S2-S5 to continuously implant the tumor tissue blocks in the tissue sampler 3 until all tumor tissue blocks in all tissue compartments 33 of the tissue sampler 3 have been implanted, thus completing the entire implantation process. Disassemble the device and sterilize it.

[0042] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A PDX model tumor tissue serial implantation device, characterized by, include: Main bushing; A pushing component is disposed at one end of the main sleeve; A tissue sampler, wherein the tissue sampler is disposed inside the main sleeve; A retaining ring is disposed on the side of the tissue sampler and located inside the main sleeve.

2. The PDX model tumor tissue serial implantation device according to claim 1, wherein, The main sleeve includes a tube body, the inside of which is provided with a tissue delivery chamber. A tissue delivery tube is installed on the side of the tube body, the inside of which is provided with a tissue delivery chamber, and a connecting tube is installed on the side of which is provided.

3. The PDX model tumor tissue serial implantation device according to claim 2, wherein, The pushing assembly includes a pushing rod, one end of which is disposed inside the tissue pushing cavity, and a piston is fixedly installed at one end of the pushing rod, the piston being slidably connected to the inner side of the tissue pushing cavity.

4. The continuous implantation device for PDX model tumor tissue according to claim 3, characterized in that, The tissue sampler includes a column body disposed inside the tissue sample delivery chamber. The side of the column body is provided with a retaining spring groove, and multiple tissue compartments are evenly provided inside the top of the column body.

5. The continuous implantation device for PDX model tumor tissue according to claim 4, characterized in that, The snap ring is engaged with the inner side of the snap ring groove.

6. The PDX model tumor tissue serial implantation device according to claim 3, wherein, The push rod has a graduated groove on its side and a limiting component is fitted on its outer side.

7. The continuous implantation device for PDX model tumor tissue according to claim 6, characterized in that, The limiting component includes a limiting ring, a through groove on one side of the limiting ring, a rotating seat fixedly installed on one side of the limiting ring, a rotating shaft fixedly installed inside the rotating seat, a pressing plate rotatably connected to the outer side of the rotating shaft, and a snap-fit ​​block fixedly installed at one end of the pressing plate.

8. The continuous implantation device for PDX model tumor tissue according to claim 7, characterized in that, The snap-fit ​​block snaps into the inner side of the scale groove.

9. A PDX model tumor tissue continuous implantation device according to claim 8, characterized in that, A torsion spring is fixedly installed on the outer side of the rotating shaft, and one end of the torsion spring is fixedly installed on the side of the pressing plate.

10. A method of using the PDX model tumor tissue continuous implantation device according to any one of claims 1-5, characterized in that, Includes the following steps: S1. Device Assembly and Pre-assembly: Assemble the main sleeve, push assembly, tissue sampler, and retaining ring. Seal the syringe containing biogel or liquid drug to the connecting tube of the main sleeve. Under sterile conditions, place the prepared 1-3 mm³ tumor tissue blocks into each tissue compartment of the tissue sampler in sequence. Push the loaded tissue sampler into the tissue delivery chamber of the main sleeve until the retaining ring engages with the retaining ring groove of the tissue sampler, so that the first tissue compartment is precisely aligned with the tissue push chamber, and the device pre-assembly is completed. S2. Pushing tumor tissue: Hold the main sleeve, pull the push rod of the push component backward to the rear stop point, and then push the push rod forward at a constant speed. The piston at the end of the push rod will push the tumor tissue block in the tissue chamber to the outlet end of the connecting tube and the tissue propulsion chamber. S3. Inject and mix the gel: Keep the push rod in place and push the externally connected syringe plunger to inject a predetermined volume of biological gel or liquid drug into the tissue propulsion cavity, so that the gel immediately encapsulates the tumor tissue block in the cavity to form a tissue-gel complex. S4. Complete a single implantation: Insert the outer cannula needle at the front end of the main cannula into the predetermined position under the skin of the experimental animal, continue to push the push rod forward to the front stop point, push the tissue-gel complex out through the outer cannula needle and inoculate it under the skin of the experimental animal, keep the push rod in place, withdraw the outer cannula needle from the experimental animal, and complete a single implantation. S5. Switch to the next tissue compartment: Pull the push rod back to the rear stop point, push the tissue sampler forward with your finger to make it slide one step, and the retaining spring will snap into another retaining spring from the current retaining slot, so as to achieve precise alignment between the next tissue compartment and the tissue delivery chamber (the bottom of the inner side of the tissue delivery chamber is provided with a retaining slot for engaging with the end face of the retaining spring). S6. Continuous implantation cycle and end: Repeat steps S2-S5 to sequentially implant the tumor tissue blocks in the tissue sampler until all tissue blocks in all tissue compartments of the tissue sampler have been implanted, thus completing the entire implantation process. Disassemble the device and sterilize it.

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