A hemp seed protein peptide that enhances immunity and serum protein levels, its preparation method, and its applications.

Abstract

This invention provides a hemp seed protein peptide that enhances immunity and serum protein levels, along with its preparation method and applications, belonging to the field of hemp seed protein peptide technology. The preparation method includes: pulverizing defatted hemp seed meal and sequentially subjecting it to hydrolysis by a complex polysaccharide enzyme, a protease, and an amidase to obtain an enzymatic hydrolysate. This hydrolysate is then processed to obtain the hemp seed protein peptide. The complex polysaccharide enzyme is a combination of cellulase, pectinase, and xylanase; the protease is pepsin; and the amidase is PNGase F. The hemp seed protein peptide of this invention has the following significant effects: 1. Significantly improves serum albumin, immunoglobulin, prealbumin, and total protein levels and immunity in middle-aged and elderly individuals, those with weak constitutions / poor nutritional status, and those recovering from illness / surgery; 2. Rapidly improves fatigue symptoms in these individuals, such as emaciation, weakness, lethargy, and poor sleep, boosting energy and relieving limb heaviness and soreness.

Description

Technical Field

[0001] This invention relates to the field of hemp seed protein peptide technology, specifically to a hemp seed protein peptide that can improve immunity and serum protein levels, its preparation method, and its application. Background Technology

[0002] Hemp seed protein peptides are derived from the dried, mature fruit of the hemp plant—the hemp seed. Hemp seeds themselves are rich in protein, containing approximately 20%-25%. Through techniques such as enzymatic hydrolysis or microbial fermentation, the large protein molecules in hemp seeds can be broken down into smaller peptide molecules that are more easily absorbed by the human body and may possess specific biological activities.

[0003] Chinese patent document CN112899330A, entitled "A Method for Preparing Antioxidant Peptides from Hemp Seed Protein," discloses a method for preparing antioxidant peptides from hemp seed protein, comprising the following steps: using an ultrasonic cell disruptor to treat hemp seeds to obtain hemp seed meal; extracting hemp seed protein using an alkaline method; preparing a Bacillus subtilis liquid fermentation medium; inoculating the hemp seed protein into the fermentation broth for culture; centrifuging the fermentation broth to collect the supernatant; separating and purifying the resulting peptide solution using a gel chromatography column; collecting the second peak of the eluted fraction; separating and identifying the peptides using liquid chromatography-mass spectrometry (LC-MS) to obtain crude hemp seed antioxidant peptides; and further purifying the crude hemp seed antioxidant peptides to obtain pure antioxidant peptides. The beneficial effects are as follows: This preparation method utilizes Bacillus subtilis fermentation, which has the advantages of high enzyme yield and recovery rate, significantly reduces costs by 50-80%, and increases yield by 10-20%. Among the pure antioxidant peptides, hemp seed antioxidant peptides have high purity, and their scavenging rate of DPPH free radicals and hydroxyl free radicals reaches over 90%. They also have thrombolytic and blood pressure-lowering effects, and have high economic value.

[0004] Chinese patent application CN120174047A, entitled "A Hemp Seed Protein Peptide for Lowering Blood Sugar and Regulating Glucose Metabolism, Its Preparation Method and Application," discloses a method for preparing hemp seed protein peptides, comprising the following steps: low-temperature supersonic fluid pulverization of hemp seeds; enzymatic pretreatment of the pulverized hemp seeds; addition of a complex protease for enzymatic hydrolysis; further enzymatic hydrolysis with PNGase F enzyme to obtain an enzymatic hydrolysate; enrichment of the enzymatic hydrolysate supernatant through membrane ultrafiltration; concentration, drying, and sterilization after tangential flow filtration to obtain the hemp seed protein peptides. The novel hemp seed protein peptides of this invention have been experimentally proven to have the following significant effects: significantly reducing fasting blood glucose levels and alleviating discomfort symptoms such as thirst, polydipsia, polyuria, and fatigue caused by hyperglycemia; significantly alleviating glucose intolerance and insulin resistance symptoms caused by hyperglycemia and reducing the risk of type 2 diabetes; and significantly reducing fasting serum insulin and glycated hemoglobin levels while increasing serum GLP-1 levels.

[0005] The above-mentioned technical solutions and the hemp seed protein peptides usually prepared in existing technologies have antioxidant, blood pressure lowering, and blood sugar lowering effects, and more functional hemp seed protein peptides need to be developed. Summary of the Invention

[0006] In view of this, the present invention provides a hemp seed protein peptide, its preparation method, and its application, to achieve the purpose of increasing serum protein and immunoglobulin levels.

[0007] To achieve the above objectives, the present invention provides a hemp seed protein peptide that enhances immunity and serum protein levels, wherein the hemp seed protein peptide comprises the following amino acid sequence: SEQ ID NO.1: FNLDSHSVIY, TTG, CY.

[0008] This invention provides a method for preparing hemp seed protein peptides that can improve immunity and serum protein levels, comprising the following steps: pulverizing defatted hemp seed meal and sequentially subjecting it to hydrolysis by a complex polysaccharide enzyme, a protease, and an amidase to obtain an enzymatic hydrolysate; then, after ultrafiltration, drying, and sterilization, obtaining hemp seed protein peptides; wherein the complex polysaccharide enzyme is a combination of cellulase, pectinase, and xylanase, the protease is pepsin, and the amidase is PNGase F.

[0009] Optionally, the weight ratio of cellulase, pectinase and xylanase is (1~3):(1~2):(1~2); the amount of the compound polysaccharide enzyme added is 0.5~2% of the weight of the defatted hemp seed meal; the pH of the compound polysaccharide enzyme during hydrolysis is 4~5.5, the hydrolysis time is 2~4h, and the temperature is 50~55℃.

[0010] Optionally, the amount of protease added is 1-4% of the weight of the defatted hemp seed meal, and the pH of the protease hydrolysis is 2-2.5, the hydrolysis time is 4-6 hours, and the temperature is 40-55°C.

[0011] Optionally, the amount of amidase added is 0.2-0.5% of the weight of defatted hemp seed meal, and the pH of the amidase hydrolysis is 7-7.5, the hydrolysis time is 2-6 hours, and the temperature is 35-40°C.

[0012] Optionally, the ultrafiltration involves passing the enzymatic hydrolysate through an ultrafiltration membrane and collecting the ultrafiltrate.

[0013] Optionally, the ultrafiltrate to be collected is a component with a molecular weight of less than 3000 Da.

[0014] Optionally, the collected ultrafiltrate is a component with a molecular weight less than 1000 Da.

[0015] The above-mentioned hemp seed protein peptide, which enhances immunity and serum protein levels, is used in the preparation of health products that regulate immunity and serum protein levels.

[0016] The above-mentioned hemp seed protein peptide, which enhances immunity and serum protein levels, is used in the preparation of a pharmaceutical composition for regulating immunity and serum protein levels.

[0017] The above-described technical solution of the present invention has at least the following beneficial effects: 1. The preparation method provided by this invention employs a three-step sequential enzymatic hydrolysis strategy: "complex polysaccharide enzyme - pepsin - PNGase F enzyme". The complex polysaccharide enzyme first degrades cell wall polysaccharides, disrupting the physical barrier and fully exposing the internal proteins; pepsin efficiently hydrolyzes the protein backbone under acidic conditions; and PNGase F enzyme specifically removes the asparagine-linked (N-linked) glycan chains of proteins. This three-step synergistic process achieves refined processing across the entire chain, from macroscopic structural disruption to microscopic molecular modification. The entire process utilizes enzymatic methods, resulting in mild conditions and high efficiency. Furthermore, the introduction of PNGase F enzyme removes glycosylation modifications, reducing the potential allergenicity of the product and making the peptide sequence more "naked," thus enhancing its biological activity.

[0018] 2. The hemp seed protein peptide provided by the present invention has been experimentally verified to significantly increase serum total protein, serum prealbumin and serum albumin, as well as immunoglobulin A and immunoglobulin G. That is, the hemp seed protein peptide provided by the present invention has the effect of increasing serum protein and immunoglobulin levels. Attached Figure Description

[0019] Figure 1 This invention provides an analysis of the differences in total protein levels among subjects in different groups before and after experimental treatment. Figure 2 This invention provides an analysis of the differences in prealbumin levels among subjects in different groups before and after experimental treatment. Figure 3 This invention provides an analysis of the differences in albumin levels among subjects in different groups before and after experimental treatment. Figure 4 This invention provides an analysis of the differences in immunoglobulin A levels among subjects in different groups before and after experimental treatment. Figure 5 This study analyzes the differences in immunoglobulin G levels among subjects in different groups before and after experimental treatment in accordance with the present invention. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the following will be described in conjunction with the accompanying drawings of the embodiments of the present invention. Figures 1-5 The technical solutions of the embodiments of the present invention will be clearly and completely described herein. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. All other embodiments obtained by those skilled in the art based on the described embodiments of the present invention are within the scope of protection of the present invention.

[0021] Example 1 This invention provides a method for preparing hemp seed protein peptides that enhance immunity and serum protein levels, comprising the following steps: Defatted hemp seed meal was pulverized and passed through an 80-mesh sieve to obtain hemp seed meal powder. The hemp seed meal powder was mixed with water at a material-to-liquid ratio of 1:15, and the pH of the system was adjusted to 5. 1.5% (by weight of defatted hemp seed meal) of a compound polysaccharide enzyme was added and hydrolyzed at 50°C for 3 hours. The enzyme was then inactivated to obtain the first enzymatic hydrolysate. The compound polysaccharide enzyme included cellulase, pectinase, and xylanase, with a weight ratio of 2:1:1. The pH of the first enzymatic hydrolysate was adjusted to 2.5, and 2% (by weight of defatted hemp seed meal) of pepsin was added and hydrolyzed at 45°C for 5 hours. The enzyme was then inactivated to obtain the second enzymatic hydrolysate. The pH of the second enzymatic hydrolysate was adjusted to 7.2, and 0.3% (by weight of defatted hemp seed meal) of PNGase F enzyme was added and hydrolyzed at 37°C for 4 hours. The enzyme was then inactivated to obtain the third enzymatic hydrolysate. The third enzymatic hydrolysate was passed through a 3000 Da ultrafiltration membrane to collect the fractions smaller than 3000 Da, which were then vacuum dried and sterilized under ultra-high pressure to obtain hemp seed protein peptides.

[0022] Example 2 This invention provides a method for preparing hemp seed protein peptides that enhance immunity and serum protein levels, comprising the following steps: Defatted hemp seed meal was pulverized and passed through a 100-mesh sieve to obtain hemp seed meal powder. The hemp seed meal powder was mixed with water at a material-to-liquid ratio of 1:12, and the pH of the system was adjusted to 4.5. 1% (by weight) of a compound polysaccharide enzyme (comprising cellulase, pectinase, and xylanase) was added and hydrolyzed at 52°C for 3.5 hours. The enzyme was then inactivated to obtain the first enzymatic hydrolysate. The compound polysaccharide enzyme included cellulase, pectinase, and xylanase in a weight ratio of 3:2:1. The pH of the first enzymatic hydrolysate was adjusted to 2.2, and 3% (by weight) of pepsin (by weight of defatted hemp seed meal) was added and hydrolyzed at 50°C for 4.5 hours. The enzyme was then inactivated to obtain the second enzymatic hydrolysate. The pH of the second enzymatic hydrolysate was adjusted to 7.3, and 0.4% (by weight) of PNGase F (by weight of defatted hemp seed meal) was added and hydrolyzed at 38°C for 5 hours. The enzyme was then inactivated to obtain the third enzymatic hydrolysate. The third enzymatic hydrolysate was passed through a 1000 Da ultrafiltration membrane to collect the fractions smaller than 1000 Da, which were then vacuum dried and sterilized under ultra-high pressure to obtain hemp seed protein peptides.

[0023] Example 3 This invention provides a method for preparing hemp seed protein peptides that enhance immunity and serum protein levels, comprising the following steps: Defatted hemp seed meal was pulverized and passed through a 120-mesh sieve to obtain hemp seed meal powder. The hemp seed meal powder was mixed with water at a material-to-liquid ratio of 1:17, and the pH of the system was adjusted to 5.5. 2% (by weight) of a compound polysaccharide enzyme (comprising cellulase, pectinase, and xylanase) was added and hydrolyzed at 55°C for 2 hours. The enzyme was then inactivated to obtain the first enzymatic hydrolysate. The compound polysaccharide enzyme included cellulase, pectinase, and xylanase in a weight ratio of 1:2:2. The pH of the first enzymatic hydrolysate was adjusted to 2.3, and 1% (by weight) of pepsin (by weight of defatted hemp seed meal) was added and hydrolyzed at 55°C for 6 hours. The enzyme was then inactivated to obtain the second enzymatic hydrolysate. The pH of the second enzymatic hydrolysate was adjusted to 7.5, and 0.5% (by weight) of PNGase F (by weight of defatted hemp seed meal) was added and hydrolyzed at 35°C for 2 hours. The enzyme was then inactivated to obtain the third enzymatic hydrolysate. The third enzymatic hydrolysate was passed through a 1000 Da ultrafiltration membrane to collect the fractions smaller than 1000 Da, which were then vacuum dried and sterilized under ultra-high pressure to obtain hemp seed protein peptides.

[0024] Example 4 This invention provides a method for preparing hemp seed protein peptides that enhance immunity and serum protein levels, comprising the following steps: Defatted hemp seed meal was pulverized and passed through a 110-mesh sieve to obtain hemp seed meal powder. The hemp seed meal powder was mixed with water at a material-to-liquid ratio of 1:16, and the pH of the system was adjusted to 4. 0.5% (by weight of defatted hemp seed meal) of a compound polysaccharide enzyme was added and hydrolyzed at 51°C for 4 hours. The enzyme was then inactivated to obtain the first enzymatic hydrolysate. The compound polysaccharide enzyme included cellulase, pectinase, and xylanase, with a weight ratio of 2:2:1. The pH of the first enzymatic hydrolysate was adjusted to 2, and 4% (by weight of defatted hemp seed meal) of pepsin was added and hydrolyzed at 40°C for 4 hours. The enzyme was then inactivated to obtain the second enzymatic hydrolysate. The pH of the second enzymatic hydrolysate was adjusted to 7, and 0.2% (by weight of defatted hemp seed meal) of PNGase F enzyme was added and hydrolyzed at 40°C for 6 hours. The enzyme was then inactivated to obtain the third enzymatic hydrolysate. The third enzymatic hydrolysate was passed through a 3000 Da ultrafiltration membrane to collect the fractions smaller than 3000 Da, which were then vacuum dried and sterilized under ultra-high pressure to obtain hemp seed protein peptides.

[0025] Analysis of the hemp seed protein peptides prepared in Examples 1-4 yielded the following core peptide sequences: SEQ ID NO.1: FNLDSHSVIY, TTG, CY.

[0026] Experiments were conducted on the hemp seed protein peptides prepared in the examples to test whether they could increase serum protein and immunoglobulin levels.

[0027] I. Experimental Methods: 1. Selection of subjects: The subjects were individuals who underwent surgery (including laparoscopic, open, and benign / malignant tumor surgeries); The patient's surgery lasted between 1 and 4 hours. The main symptoms include: easy fatigue (such as weakness, loss of appetite, poor concentration, depression, tension, anxiety, etc., evaluated according to the fatigue scale), drowsiness / insomnia (evaluated according to the sleep scale), and weakened immunity. Age between 35 and 75 years old, gender not limited; Those who agree to participate in this research.

[0028] 2. Testing Methods Subjects were randomly assigned to two groups: a placebo group (Group A) and an experimental group (Group E). The placebo group (Group A) received a placebo capsule, while the experimental group (Group E) received capsules containing hemp seed protein peptides. The placebo was a residual food additive without hemp seed protein peptides, identical in appearance, shape, odor, and size to the capsules containing hemp seed protein peptides. Dosage: Twice daily, 30 minutes after breakfast and dinner.

[0029] The trial treatment period was 30 days. Subjects were randomly assigned to groups. After enrollment, drug management personnel distributed trial capsules to the subjects, who then consumed the capsules according to the recommended dosage and method. The trial set two monitoring points: day 1 before treatment (T1) and day 30 of treatment (T2). Subjects were followed up and their indicators were measured. During the follow-up visits, nutritional indicators were recorded. The trial product was distributed and collected, and the trial product was taken continuously for 30 days.

[0030] Nutritional indicators include blood protein levels, including total protein, albumin, and prealbumin levels.

[0031] Total protein (TP) is a key indicator for measuring protein levels in an individual's body. It includes albumin and globulins and is an important biochemical parameter reflecting liver synthetic function and nutritional status. Changes in total protein levels are generally related to an individual's nutrient absorption, protein synthesis, and metabolic status. Therefore, studying changes in serum total protein can assess the impact of experimental samples on improving the nutritional status of subjects.

[0032] Albumin's primary function is to maintain plasma colloid osmotic pressure and aid in the transport of various substances (such as hormones, fatty acids, and drugs). Albumin reflects the body's overall protein synthesis capacity and long-term nutritional status. Due to its long-term stability, albumin is often used to assess patients with chronic diseases or long-term malnutrition. Changes in albumin typically indicate changes in the patient's overall health, including liver function and the progression of chronic diseases. Therefore, while both prealbumin and albumin are related to nutritional status, they have different focuses. Prealbumin is suitable for monitoring short-term nutritional changes, while albumin is better suited for assessing long-term nutritional status. Clinically, both are often used in combination for a more comprehensive nutritional and health assessment.

[0033] Prealbumin (PA) and albumin are both important protein indicators for assessing nutritional status, but they differ significantly in function, half-life, and uses. Prealbumin's primary function is to transport thyroid hormones and retinol (vitamin A). Its main physiological role is closely related to nutritional status assessment, and it is one of the rapidly reacting proteins synthesized by the liver. Prealbumin levels can reflect short-term changes in nutrient intake and protein metabolism. In clinical studies, due to its short half-life, prealbumin is often used to monitor rapid changes in a patient's nutritional status during short-term nutritional interventions or treatments, making it particularly suitable for nutritional assessment in acute-phase patients or postoperative recovery.

[0034] Immune function indicators include the levels of immunoglobulin A and immunoglobulin G.

[0035] Immunoglobulins (Ig) are a class of proteins with antibody activity produced by the human immune system. They are secreted by plasma cells that differentiate from B lymphocytes. Their main function is to recognize and neutralize pathogens (such as bacteria and viruses) or foreign harmful substances, and they are the core components of humoral immunity.

[0036] Immunoglobulin A (IgA) is a key component of the human mucosal immune system, widely distributed in the mucous membranes and secretions (such as saliva and breast milk) of the respiratory, digestive, and genitourinary tracts. It is primarily responsible for preventing pathogens from adhering to and invading the body, serving as the "first line of defense" against infection. Its unique structure (such as secretory IgA) and function make it irreplaceable in local immunity.

[0037] Immunoglobulin G (IgG) is the most abundant antibody type in human serum (approximately 75-80%) and a core member of the immunoglobulin family. Produced by B cells, it recognizes and neutralizes pathogens (such as bacteria and viruses), clearing foreign substances by activating the complement system and promoting phagocytosis. II. Experimental Results 1. Nutritional and immune function indicators of subjects before treatment 1.1 Nutritional indicators of subjects before treatment

[0038] Note: There were no significant differences in data before and after treatment among the groups. Total protein, albumin, and prealbumin were measured in units of (g / L) and (mg / L), respectively. Group A, n=38; Group E, n=36 As shown in Table 1, there were no significant differences in serum total protein, albumin, and prealbumin levels among the subjects in each group before treatment.

[0039] 1.2 Immune function indicators of subjects before treatment

[0040] Group A, n=38; Group E, n=36 2. Nutritional and immune function indicators of subjects in each group before and after treatment 2.1 Nutritional indicators of subjects in each group before and after treatment

[0041] Group A, n=38; Group E, n=36 2.1.1 Total protein levels in each group before and after treatment Analysis of differences in total protein levels among subjects before and after treatment is shown in the figure. Figure 1 .

[0042] Depend on Figure 1 As shown in Table 3, compared with the pre-treatment data, the serum total protein, albumin and prealbumin levels of subjects in groups A and E increased significantly, indicating that the serum total protein levels of subjects in each group recovered well within 30 days, and that the intake of the test sample had no negative impact on the nutritional recovery of the subjects.

[0043] 2.1.2 Prealbumin levels in each group before and after treatment Analysis of differences in prealbumin levels among subjects before and after treatment is shown in the figure. Figure 2 .

[0044] Depend on Figure 2 As shown in Table 3, compared with the pre-treatment data, the serum prealbumin levels of subjects in groups A and E increased significantly. This indicates that the serum prealbumin levels of subjects in each group recovered well within 30 days.

[0045] 2.1.3 Albumin levels in each group before and after treatment Analysis of differences in albumin levels among subjects before and after treatment is shown in the figure. Figure 3 .

[0046] Depend on Figure 3 As shown in Table 3, compared with the pre-treatment data, the serum albumin levels of subjects in groups A and E increased significantly. This indicates that the serum albumin levels of subjects in each group recovered well within 30 days.

[0047] 2.2 Immune function indicators of subjects in each group before and after treatment

[0048] Group A, n=38; Group E, n=36 2.2.1 IgA immunoglobulin levels in each group before and after treatment Analysis of differences in IgA immunoglobulin levels among subjects before and after treatment is shown in the figure. Figure 4 .

[0049] Depend on Figure 4 As shown in Table 4, compared with the pre-treatment data, the immunoglobulin A levels in groups A and E were significantly increased. This indicates that the immunoglobulin A synthesis function of the subjects in each group was significantly improved within 30 days.

[0050] 2.2.2 IgG immunoglobulin levels in each group before and after treatment Analysis of differences in IgG immunoglobulin levels among subjects before and after treatment is shown in the figure. Figure 5 .

[0051] Depend on Figure 5 As shown in Table 4, compared with the pre-treatment data, the immunoglobulin G levels in groups A and E were significantly increased. This indicates that the immunoglobulin G synthesis function of the subjects in each group was significantly improved within 30 days.

[0052] 3. Nutritional and immune function indicators of subjects in each group after treatment 3.1 Nutritional indicators of subjects in each group after treatment

[0053] Group A, n=38; Group E, n=36 As shown in Table 5, after 30 days of treatment, compared with group A, the serum total protein level of subjects in group E increased significantly (P<0.05), indicating that compared with natural recovery, the hemp seed protein peptide provided by the present invention can effectively improve the serum total protein level.

[0054] As shown in Table 5, after 30 days of treatment, compared with group A, the serum prealbumin level in group E was significantly increased (P<0.05), reaching 298.59±28.24 mg / L. This indicates that compared with natural recovery, the hemp seed protein peptide provided by this invention can effectively increase serum prealbumin levels.

[0055] As shown in Table 5, after 30 days of treatment, compared with group A, the serum albumin level of subjects in group E increased significantly (P<0.05), indicating that the hemp seed protein peptide provided by the present invention can effectively improve serum albumin level compared with natural recovery.

[0056] The hemp seed protein peptide provided by this invention has a significant effect on increasing serum protein levels.

[0057] 3.2 Immune function indicators of subjects in each group after treatment

[0058] Group A, n=38; Group E, n=36 As shown in Table 6, after 30 days of treatment, compared with group A, group E showed that immunoglobulin A and immunoglobulin G could effectively improve the immune protein synthesis function and increase immunoglobulin levels.

[0059] The above are preferred embodiments of the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A hemp seed protein peptide that enhances immunity and serum protein levels, characterized in that, The hemp seed protein peptide includes the following amino acid sequence: SEQ ID NO.1: FNLDSHSVIY, TTG, CY.

2. A method for preparing hemp seed protein peptides as described in claim 1, characterized in that, Includes the following steps: Defatted hemp seed meal was pulverized and then subjected to hydrolysis by a compound polysaccharide enzyme, a protease, and an amidase to obtain an enzymatic hydrolysate. After ultrafiltration, drying, and sterilization, hemp seed protein peptides were obtained. The compound polysaccharide enzyme was obtained by combining cellulase, pectinase, and xylanase, the protease was pepsin, and the amidase was PNGase F.

3. The method for preparing hemp seed protein peptides that enhance immunity and serum protein levels according to claim 2, characterized in that, The weight ratio of cellulase, pectinase and xylanase is (1~3):(1~2):(1~2); the amount of compound polysaccharide enzyme added is 0.5~2% of the weight of defatted hemp seed meal; the pH of the compound polysaccharide enzyme hydrolysis is 4~5.5, the hydrolysis time is 2~4h, and the temperature is 50~55℃.

4. The method for preparing hemp seed protein peptides that enhance immunity and serum protein levels according to claim 2, characterized in that, The amount of protease added is 1-4% of the weight of the defatted hemp seed meal. The protease is hydrolyzed at a pH of 2-2.5, a hydrolysis time of 4-6 hours, and a temperature of 40-55°C.

5. The method for preparing hemp seed protein peptides that enhance immunity and serum protein levels according to claim 2, characterized in that, The amount of amidase added is 0.2-0.5% of the weight of defatted hemp seed meal. The pH of the amidase hydrolysis is 7-7.5, the hydrolysis time is 2-6 hours, and the temperature is 35-40℃.

6. The method for preparing hemp seed protein peptides that enhance immunity and serum protein levels according to claim 2, characterized in that, The ultrafiltration process involves passing the enzymatic hydrolysate through an ultrafiltration membrane and collecting the ultrafiltrate.

7. The method for preparing hemp seed protein peptides with the effect of improving immunity and serum protein levels according to claim 6, characterized in that, The ultrafiltrate to be collected consists of components with a molecular weight less than 3000 Da.

8. The method for preparing hemp seed protein peptides with the effect of improving immunity and serum protein levels according to claim 6, characterized in that, The collected ultrafiltrate consists of components with a molecular weight less than 1000 Da.

9. The hemp seed protein peptide as described in claim 1, which enhances immunity and serum protein levels, is used in the preparation of a health product for regulating immunity and serum protein levels.

10. The preparation of a pharmaceutical composition for regulating immunity and serum protein levels using the hemp seed protein peptide as described in claim 1.

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