A composition of natural origin and its use in the treatment and / or prevention of inflammation of the skin in infants and young children

Abstract

This invention belongs to the technical field of cosmetics and topical skin preparations, specifically relating to a naturally derived composition and its application in treating and / or preventing skin inflammation in infants and young children. The naturally derived composition provided by this invention includes egg yolk oil, flaxseed oil, and borage seed oil; the mass ratio of the egg yolk oil, flaxseed oil, and borage seed oil is (1~50):(0.1~10):(0.1~10). The composition provided by this invention can synergistically act on the key mechanisms and targets that trigger contact dermatitis in infants and young children, effectively preventing and treating contact dermatitis and maintaining healthy skin. The composition provided by this invention is hormone-free, antibiotic-free, naturally derived, highly safe, mild and non-irritating, and will not induce drug resistance; it can be widely used in cosmetics and topical skin preparations.

Description

Technical Field

[0001] This invention belongs to the field of cosmetics and topical skin preparations, specifically relating to a naturally derived composition and its application in the treatment and / or prevention of skin inflammation in infants and young children. Background Technology

[0002] Infants' skin has unique physiological structure and functional characteristics. Their stratum corneum is thin, their skin barrier function is not fully developed, and their tolerance is poor. Their ability to resist various external stimuli is far lower than that of adults. Therefore, skin inflammation problems are extremely common in infants and young children. Among them, infant contact dermatitis is one of the most common skin diseases in clinical practice, which seriously affects the skin health and quality of life of infants and young children, and also brings a lot of trouble to parents.

[0003] Understanding the pathogenesis of contact dermatitis in infants and young children is fundamental to solving this type of skin problem. It differs fundamentally from eczema (atopic dermatitis) in its mechanism and needs to be clearly distinguished to avoid deviations in treatment. Eczema (atopic dermatitis) is a congenital skin barrier defect and dysfunction caused by genetic factors (mainly filaggrin gene mutation). Under the combined action of environmental stimuli, allergens, and microbial colonization, it triggers an immune disorder centered on Th2 immune dominance activation, which in turn releases a large number of inflammatory and pruritus factors, ultimately manifesting as a chronic inflammatory skin disease with symptoms such as dryness, erythema, papules, exudation, thickening, and intense itching. Infantile contact dermatitis, on the other hand, is a skin inflammation caused directly by continuous external physical or chemical stimulation. It is a non-allergic inflammation, and its core triggers include moisture maceration, friction, saliva, urine, feces, and chemicals. These external stimuli directly damage the fragile skin barrier of infants and young children, causing epidermal inflammation. Clinically, it mainly manifests as erythema, dryness, papules, desquamation, erosion, and exudation, and commonly occurs in easily irritated areas such as the perioral area, buttocks, skin folds, face, and hands.

[0004] Contact dermatitis in infants presents in several common forms, with diaper rash (red bottom), perioral erythema (drool rash), and neck / axillary / groin erythema (intertrigo) being the most prevalent. Specifically, diaper rash is caused by prolonged irritation from urine and feces in diaper-covered areas such as the buttocks, perineum, and groin. This, combined with the moist environment and diaper friction, damages the skin barrier and causes inflammation. In severe cases, secondary infections such as Candida and bacteria can occur, manifesting as large areas of erythema, epidermal damage, erosion, and stinging. Perioral erythema is caused by prolonged stimulation of the skin around the infant's mouth by salivary enzymes, the moist maceration from saliva and milk, and further damage from friction such as wiping with tissues, leading to a compromised skin barrier. When the skin is damaged, the resulting moist environment easily breeds pathogenic bacteria such as Staphylococcus aureus and Candida albicans, which in turn aggravates the inflammation. This manifests as erythema, dryness, roughness, dense papules, and desquamation around the mouth and chin. In severe cases, erosion, exudation, and crusting may occur. Intertrigo in skin folds such as the neck, armpits, and groin is caused by the fact that sweat does not evaporate easily in these areas. Long-term stimulation and maceration by sweat and friction between skin leads to damage to the skin barrier and epidermal inflammation, manifesting as epidermal damage, redness, erosion, and significant pain.

[0005] With increasing demand for healthy infant skin, various products targeting contact dermatitis-related skin problems such as diaper rash and drool rash have appeared on the market, but their treatment and prevention effects are unsatisfactory. Furthermore, the current market for topical infant skin products also poses safety risks. Some products, in pursuit of short-term anti-inflammatory and antipruritic effects, illegally add prohibited ingredients such as hormones and antibiotics. While these ingredients can quickly relieve symptoms, long-term use can lead to drug resistance in infants, disrupt the skin's microecological balance, and may even cause systemic adverse reactions through skin absorption, seriously endangering the health of infants. Summary of the Invention

[0006] The purpose of this invention is to provide a naturally derived composition and its application in the treatment and / or prevention of skin inflammation in infants and young children. The naturally derived composition provided by this invention has excellent effects of isolating irritants, repairing barriers, anti-inflammatory and soothing, antibacterial and moisturizing, and has good efficacy in preventing contact dermatitis in infants and young children. It is hormone-free, antibiotic-free, naturally derived, highly safe, mild and non-irritating, and will not produce drug resistance. It can be widely used in cosmetics and topical skin preparations.

[0007] To achieve the above objectives, the present invention provides the following technical solution: The present invention provides a naturally derived composition comprising egg yolk oil, flaxseed oil, and borage seed oil; wherein the mass ratio of the egg yolk oil, flaxseed oil, and borage seed oil is (1~50):(0.1~10):(0.1~10).

[0008] Preferably, the mass ratio of egg yolk oil, flaxseed oil, and borage seed oil is (5~30):(0.5~5):(0.5~5).

[0009] Preferably, the mass ratio of egg yolk oil, flaxseed oil, and borage seed oil is 12:1:1.

[0010] The present invention provides the use of the natural source composition described above in the preparation of products for treating and / or preventing contact dermatitis in infants and young children, wherein the products for treating and / or preventing contact dermatitis in infants and young children include cosmetics or topical skin preparations.

[0011] Preferably, the product for treating and / or preventing contact dermatitis in infants and young children contains the natural source composition at a mass percentage of 1 to 100%.

[0012] Preferably, the product for treating and / or preventing contact dermatitis in infants and young children contains 5-30% by mass of the naturally derived composition.

[0013] This invention provides a cosmetic product comprising the naturally derived composition described in the above technical solution.

[0014] Preferably, the product type of the cosmetic includes any one of cream, lotion, serum, toner, mask, gel, jelly, and ointment; the natural source composition in the cosmetic has a mass percentage of 1 to 100%.

[0015] This invention provides a topical skin preparation comprising the naturally derived composition described in the above technical solution.

[0016] Preferably, the product type of the topical skin preparation includes any one of creams, ointments, gels, patches, and sprays; the natural source composition in the topical skin preparation has a mass percentage of 1 to 100%.

[0017] This invention provides a naturally derived composition comprising egg yolk oil, flaxseed oil, and borage seed oil; wherein the mass ratio of the egg yolk oil, flaxseed oil, and borage seed oil is (1~50):(0.1~10):(0.1~10). The naturally derived composition provided by this invention can synergistically prevent and treat contact dermatitis in infants and young children, and maintain healthy skin. Firstly, targeting the root cause of contact dermatitis in infants and young children—external physical / chemical irritants—the naturally derived composition provided by this invention uses a pure oil formulation. Utilizing the hydrophobic properties of oils, it forms a dense hydrophobic protective film on the surface of the infant's skin, effectively isolating various external irritants such as moisture, friction, saliva, urine, and feces from the source, blocking direct contact between the irritant and the skin, fundamentally reducing the possibility of skin barrier damage, and achieving a preventive effect against contact dermatitis in infants and young children. This is a prerequisite for solving this type of skin problem. Secondly, regarding the core pathological link of skin barrier damage caused by external stimuli, the egg yolk oil, flaxseed oil, and borage seed oil in the natural source composition provided by this invention have excellent barrier repair effects. Egg yolk oil is rich in lecithin and contains small amounts of fat-soluble nutrients such as vitamin A. Lecithin can repair damaged cell membrane structures, and vitamin A can regulate keratinocyte differentiation; the two work synergistically to promote the repair and regeneration of the skin barrier. Borage seed oil is rich in gamma-linolenic acid (GLA), a precursor to ceramides (key lipids in the skin barrier), which can promote the synthesis of ceramides in the stratum corneum, regulate the lipid arrangement of the stratum corneum, enhance skin barrier function, and reduce transepidermal water loss. Flaxseed oil can supplement the skin with the unsaturated fatty acids needed by the skin, further strengthening the integrity of the skin barrier. The synergistic effect of these three ingredients can effectively repair skin barrier damage in infants and young children caused by external stimuli and friction, restore the skin's normal defense function, and provide a basic guarantee for skin health. Thirdly, regarding the epidermal inflammatory response caused by skin barrier damage, the natural source composition provided by this invention has significant anti-inflammatory and soothing effects. Its active ingredients can inhibit the secretion of inflammatory factors such as IL1, IL6, IL8, and TNF-α by keratinocytes, while simultaneously inhibiting the release of inflammatory mediators such as histamine and leukotrienes from mast cells. This blocks the transmission process of the inflammatory response, relieving inflammatory symptoms such as erythema, swelling, stinging, and itching, preventing further aggravation of inflammation, and accelerating the resolution of skin inflammation, thus achieving a therapeutic effect on contact dermatitis in infants and young children. Specifically, the gamma-linolenic acid in borage seed oil can be metabolized into prostaglandin E1 (PGE1), which can inhibit the release of pro-inflammatory mediators, reduce skin inflammatory responses, further enhance the anti-inflammatory and soothing effects, and assist in relieving skin inflammation.Then, addressing the issue of pathogenic bacteria such as Staphylococcus aureus and Candida albicans easily colonizing the skin barrier in humid environments, thus exacerbating inflammation, the natural-derived composition provided by this invention has good antibacterial effects. It can effectively inhibit the growth and reproduction of the aforementioned pathogenic bacteria, disrupt their living environment, reduce the risk of microbial infection, and prevent further deterioration of inflammation due to infection. Simultaneously, it protects the skin's microecological balance, creating favorable conditions for skin barrier repair and forming a synergistic effect of "repair-antibacterial-anti-inflammatory." Finally, addressing the dryness and flaking problems that easily occur after skin barrier damage, the natural oil components in the natural-derived composition provided by this invention have excellent moisturizing effects. They can form a water-locking protective film on the skin surface, reduce skin moisture loss, replenish the skin's required oils and moisture, alleviate symptoms such as dryness, roughness, and flaking, maintain the skin's hydration, further strengthen the skin barrier function, promote skin recovery, and reduce the recurrence rate of skin problems. The natural-derived composition provided by this invention, by controlling the mass ratio of egg yolk oil, flaxseed oil, and borage seed oil at (1~50):(0.1~10):(0.1~10), achieves a synergistic effect among the three components. This optimizes the balance of the five major functions of the natural-derived composition: hydrophobic isolation, barrier repair, anti-inflammatory soothing, antibacterial, and moisturizing, comprehensively covering the entire pathogenesis of contact dermatitis in infants and young children. Furthermore, the natural-derived composition provided by this invention is naturally derived, free of prohibited ingredients such as hormones and antibiotics, ensuring high safety, gentleness, and non-irritation, and preventing drug resistance. It is suitable for the delicate skin of infants and young children and can be widely used in cosmetics and topical skin preparations. This effectively addresses the technical shortcomings of existing products, such as poor efficacy and insufficient safety, providing a novel technical solution for the prevention and treatment of contact dermatitis in infants and young children. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0019] Figure 1 The migration rate distribution of HaCaT cells in samples 1-8 of Example 2 is shown. Figure 2 This is a graph showing the results of samples 1-8 in Example 3 inhibiting TNF-α secretion in HaCaT cells; Figure 3 This is a graph showing the results of samples 1-8 in Example 3 inhibiting IL-8 secretion in HaCaT cells; Figure 4 This is a graph showing the results of samples 1-8 in Example 3 inhibiting IL-1β secretion in HaCaT cells; Figure 5 The graph shows the changes in skin water content and epidermal thickness of samples 1-8 in Example 6; Figure 6 The transdermal water loss rate (TEWL) of samples 1-8 in Example 6 and Graph showing the results of value changes. Detailed Implementation

[0020] The present invention provides a naturally derived composition comprising egg yolk oil, flaxseed oil, and borage seed oil; wherein the mass ratio of the egg yolk oil, flaxseed oil, and borage seed oil is (1~50):(0.1~10):(0.1~10).

[0021] In this invention, unless otherwise specified, all raw materials / components used in the preparation are commercially available products well known to those skilled in the art.

[0022] In this invention, the preferred mass ratio of egg yolk oil, flaxseed oil, and borage seed oil is (2~40):(0.2~8):(0.2~8), more preferably (5~30):(0.5~5):(0.5~5), further preferably (8~20):(0.6~3):(0.6~3), even more preferably (10~15):(0.8~2):(0.8~2), and most preferably 12:1:1.

[0023] In this invention, the egg yolk oil, also known as phoenix oil, contains cholesterol, triglycerides (such as oleic acid), and essential fatty acids (such as linoleic acid), which are important components of the epidermis. These components strengthen the stratum corneum, repair damaged barriers, and provide good barrier repair and moisturizing effects. The egg yolk oil also accelerates skin cell regeneration and promotes healing of minor burns, scalds, chapped skin, or ulcers. The protective film it forms isolates external stimuli and bacterial infections, while providing lipids necessary for tissue repair and shortening healing time. The linoleic acid and phospholipids in the egg yolk oil have good anti-inflammatory effects, reducing skin redness, swelling, itching, and other inflammatory reactions. The egg yolk oil contains abundant caprylic acid and capric acid, two natural medium-chain fatty acids. These acids can penetrate the cell membranes of bacteria or fungi, disrupting their structure and inhibiting pathogen growth. They have a significant inhibitory effect on Candida albicans and Staphylococcus aureus and are often used as an adjunct treatment for fungal infections or skin problems. This invention does not have strict requirements regarding the source of the egg yolk oil; it can be purchased regularly or extracted and prepared independently. The present invention does not impose strict requirements on the preparation method of the egg yolk oil; it can be conventional extraction, supercritical carbon dioxide extraction, or bio-fermentation extraction.

[0024] In this invention, the flaxseed oil contains 25%–30% of the essential fatty acid α-linolenic acid (Omega-3). Omega-3 and its metabolite SDA are important precursors for the synthesis of EPA and DHA in the body. EPA can further generate prostaglandin GE3, which plays an important role in regulating skin inflammatory responses. Oils rich in Omega-3 have a significant effect on improving skin redness. This invention does not have strict requirements on the source of the flaxseed oil; it can be purchased conventionally or extracted and prepared by the user. This invention also does not have strict requirements on the preparation method of the flaxseed oil; it can be obtained through conventional extraction, supercritical carbon dioxide extraction, or bio-fermentation extraction.

[0025] In this invention, the borage seed oil is rich in the essential fatty acid linoleic acid, an important component of the intercellular lipid complex of the stratum corneum, which strengthens the stratum corneum. Furthermore, the borage seed oil of this invention is the natural oil with the highest γ-linolenic acid (GLA) content (approximately 23%) in nature. Breast milk is rich in GLA and is the most important source of GLA for infants. GLA can inhibit pro-inflammatory mediators and reduce leukotriene production through PGE1 and 15-HETrE. The borage seed oil of this invention can improve skin inflammation symptoms, promote rehydration of dry skin, repair skin barrier damage, and reduce transepidermal water loss. This invention does not have strict requirements regarding the source of the borage seed oil; it can be purchased conventionally or extracted and prepared at home. This invention also does not have strict requirements regarding the preparation method of the borage seed oil; it can be extracted conventionally, by supercritical carbon dioxide extraction, or by bio-fermentation.

[0026] Based on the pathogenesis of contact dermatitis, the mechanism of action of the natural source composition provided by this invention mainly includes the following five aspects: First, the natural source composition provided by this invention is a pure oil substance with good hydrophobic isolation properties, which can isolate external stimuli such as moisture, friction, saliva, urine, and feces from the source; Second, the natural source composition provided by this invention has good barrier repair properties, which can effectively repair the skin barrier damage caused by external stimuli and friction in infants; Third, the natural source composition provided by this invention has good anti-inflammatory and soothing properties, which can effectively inhibit the secretion of inflammatory factors such as IL1, IL6, IL8 and TNF-α by keratinocytes, as well as the release of inflammatory mediators such as histamine and leukotrienes by mast cells; Fourth, the natural source composition provided by this invention has good antibacterial properties, which can effectively inhibit the growth of pathogenic bacteria such as Staphylococcus aureus and Candida albicans; Fifth, the natural source composition provided by this invention has good moisturizing properties, which can effectively relieve skin dryness and peeling caused by skin barrier damage. In summary, the natural composition provided by this invention has excellent effects of isolating irritants, repairing barriers, anti-inflammatory and soothing, antibacterial and moisturizing. It is free of hormones and antibiotics, is naturally derived, has high safety, is mild and non-irritating, and will not produce drug resistance. It can be widely used in cosmetics and topical skin preparations.

[0027] This invention provides the application of the natural source composition described in the above technical solution in the preparation of cosmetics.

[0028] The present invention provides the use of the natural source composition described above in the preparation of products for treating and / or preventing contact dermatitis, wherein the products for treating and / or preventing contact dermatitis in infants and young children include cosmetics or topical skin preparations.

[0029] The present invention provides the use of the natural source composition described above in the preparation of products for treating and / or preventing contact dermatitis in infants and young children, wherein the products for treating and / or preventing contact dermatitis in infants and young children include cosmetics or topical skin preparations.

[0030] In this invention, the mass percentage of the natural source composition in the product for treating and / or preventing contact dermatitis in infants is preferably 1 to 100%, more preferably 3 to 80%, further preferably 5 to 30%, and most preferably 10 to 20%.

[0031] This invention provides a cosmetic product comprising the naturally derived composition described in the above technical solution.

[0032] In this invention, the product type of the cosmetic preferably includes any one of creams, lotions, serums, toners, masks, gels, jelly, and ointments. The mass percentage of the naturally derived composition in the cosmetic is preferably 1-100%, more preferably 3-80%, further preferably 5-30%, and most preferably 10-20%. In this invention, the cosmetic can be any one of the following dosage forms: oil-in-water, water-in-oil, water-in-oil-in-water, oil-in-water-in-oil, and pure oil, preferably a pure oil dosage form.

[0033] This invention provides a topical skin preparation comprising the naturally derived composition described in the above technical solution.

[0034] In this invention, the product type of the topical skin preparation preferably includes any one of creams, ointments, gels, patches, and sprays. The mass percentage of the natural-derived composition in the topical skin preparation is preferably 1-100%, more preferably 3-80%, further preferably 5-30%, and most preferably 10-20%. In this invention, the topical skin preparation can be any one of oil-in-water, water-in-oil, water-in-oil-in-water, oil-in-water-in-oil, or pure oil dosage forms, preferably a pure oil dosage form.

[0035] In summary, the natural source composition provided by this invention combines egg yolk oil, flaxseed oil, and borage seed oil in a reasonable ratio, which can achieve a synergistic effect. It can work synergistically on the key mechanisms and targets that cause contact dermatitis in infants and young children, effectively prevent and treat contact dermatitis in infants and young children, and maintain healthy skin.

[0036] To further illustrate the present invention, the technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0037] Example 1: Hydrophobicity Test 1. Test Sample Preparation method: Egg yolk oil, flaxseed oil, and borage seed oil are mixed in the mass ratios shown in Table 1 to obtain compositions 1-8. The specific mass ratios of each component in compositions 1-8 are shown in Table 1.

[0038] Table 1. Mass ratio of each component in the composition

[0039] 2. Testing instruments Dataphysics OAC15EC (Germany) 3. Testing Methods Place suitable artificial pigskin on the instrument, apply composition 1 to 8, test each composition in parallel 3 times, keep the application uniform each time, drop water droplets on the sample surface, and obtain the surface contact angle of each sample by image capture and instrument analysis. The results are shown in Table 2.

[0040] Table 2. Surface contact angle results of compositions 1-8

[0041] Note: A surface contact angle < 90° indicates that the sample is hydrophilic; a surface contact angle > 90° indicates that the sample is hydrophobic. As shown in Table 2, the surface contact angles of compositions 1 to 8 are all >90°, indicating that compositions 1 to 8 are all hydrophobic. The natural source compositions provided by this invention can effectively isolate external stimuli.

[0042] Example 2: Cell Repair Experiment 1. Test Sample Preparation method: Mix 1.4 wt% of the compositions 1-8 in Table 1 with 98.6 wt% DMEM culture medium to obtain test samples 1-8.

[0043] 2. Testing Methods Collect HaCaT cells (human immortalized keratinocytes) in the logarithmic growth phase and adjust the cell density to 5 × 10⁻⁶. 5 Cells were seeded at a density of 1 / mL in 6-well plates and cultured overnight. The culture medium was aspirated from the plates, and a micropipette was used to streak the cells in the central region of the growth zone. The central portion was removed, and the streaked portion was rinsed with PBS. 2 mL of fresh serum-free DMEM medium was added to the blank control group, and 2 mL of test sample was added to the drug-treated group. The cells were incubated for 24 h. The width of the streaks was then observed under a microscope and photographed. Cell migration rate was calculated based on cell migration distance. The results are shown in Table 3. Figure 1 .

[0044] Table 3 Cell migration rates in different treatment groups

[0045] Note: Compared with the blank control group. Compared with sample 1, # P <0.05.

[0046] The total content of active ingredients in samples 1-8 is the same, as shown in Table 3 and Figure 1 It can be seen that, compared with the blank control group, the increase in HaCaT cell migration rate in samples 1-3 of the drug-treated groups was significantly different. This indicates that egg yolk oil, flaxseed oil, and borage seed oil all have good cell repair effects, with egg yolk oil (sample 1) showing the best effect. Furthermore, compared with sample 1, the combinations of samples 4-7 further improved the migration rate of HaCaT cells, and the differences were statistically significant. # P The value <0.05 indicates that the natural-derived composition of the present invention has a better effect on repairing keratinocytes than a single active ingredient. The composition has a synergistic effect and can more effectively improve the healing speed of keratinocytes. Among them, the effect of sample 4 is better than that of samples 5-7, indicating that the cell repair effect is optimal when the mass ratio of egg yolk oil, flaxseed oil, and borage seed oil is 12:1:1, which can effectively repair damage to the keratin barrier.

[0047] Example 3: Cellular Anti-inflammatory Experiment 1. Test Sample Same as Example 2 2. Testing Methods RAW 264.7 cells were planted at a density of 5 × 10⁶ cells per well. 5 Cells were seeded at a density of 1000 cells / well in 24-well plates and cultured at 37°C in a 5% CO2 incubator for 24 h. After culture, the culture medium was aspirated from the cell plates, and the cells were randomly divided into a blank control group, a model group, and a drug treatment group. Except for the blank control group, 1 mg / mL LPS was added to each well to establish a cell inflammation model. The drug treatment group was given the test sample, and the blank control group was given an equal volume of serum-free culture medium. The cells were cultured for another 48 h. After 48 h, the cell supernatant was collected, and the inflammatory factors TNF-α, IL-8, and IL-1β were detected according to the ELISA kit instructions. The contents of TNF-α, IL-8, and IL-1β in the cell supernatant were calculated. The results are shown in Table 4. Figure 2 , Figure 3 and Figure 4 As shown.

[0048] Table 4. Results of inflammatory factor content measurement in different treatment groups

[0049] Note: Compared with the model group. Compared with sample 1, # P <0.05.

[0050] The total content of active ingredients in samples 1-8 is the same, according to Table 4 and Figure 2 , Figure 3 , Figure 4 It can be seen that, compared with the model group, egg yolk oil, flaxseed oil, and borage seed oil alone can significantly inhibit the secretion of TNF-α, IL-8, and IL-1β by HaCaT cells, and the differences are statistically significant. Of these, sample 1 (egg yolk oil) showed the best anti-inflammatory effect; furthermore, compared with sample 1, samples 4-8 showed significantly enhanced inhibitory effects on the secretion of TNF-α, IL-8, and IL-1β by HaCaT cells. # P The value <0.05 indicates that the anti-inflammatory effect of the naturally derived composition of this invention is superior to that of a single active ingredient, exhibiting a synergistic effect and significantly inhibiting the secretion of inflammatory factors. Sample 4 showed better results than samples 5-8, indicating that the anti-inflammatory and soothing effect was optimal when the mass ratio of egg yolk oil, flaxseed oil, and borage seed oil was 12:1:1.

[0051] Example 4: Antibacterial Experiment 1. Test Sample Preparation method: Mix 14wt% of the compositions 1-8 in Table 1 with 86wt% PBS phosphate buffer (0.03 mol / L, pH=7.2-7.4) to obtain test samples 1-8.

[0052] 2. Test strain Staphylococcus aureus (ATCC 6538), Candida albicans (ATCC 10231).

[0053] 3. Culture medium Staphylococcus aureus is cultured on nutrient agar medium, while Candida albicans is cultured on Sabouraud agar medium.

[0054] 4. Testing Methods Take a fresh 24-hour slant culture of the test bacteria, wash it off with PBS, and dilute it with PBS to approximately 5.0 × 10⁻⁶. 5 CFU / mL ~4.5×10 6 Prepare a CFU / mL bacterial suspension. Take a sterile test tube, add 5 mL of the test sample, incubate at 20°C for 5 minutes, then add 0.1 mL of the test bacterial suspension and mix quickly. Inoculate 1 mL of the test bacterial and sample mixture into two Petri dishes, pouring the culture medium over them. Simultaneously, perform a parallel test using PBS instead of the sample as a positive control. The positive control should have a colony count of 1.0 × 10⁻⁶. 4 CFU / mL ~ 9.0×10 4 The concentrations were between CFU / mL. PBS and culture medium from the same batch were used as negative controls. All test and control samples were incubated at 36℃. Staphylococcus aureus was incubated for 48 hours to observe the final results; Candida albicans was incubated for 72 hours to observe the final results. The inhibition rate was calculated, and the experimental results are shown in Table 5. The formula for calculating the inhibition rate is as follows: ; In the formula: X—Antibacterial rate, % A0—Positive control group recovered bacterial count, in CFU / mL; A1—The amount of bacteria recovered in the experimental group, in units of CFU / mL.

[0055] Table 5 Results of Antibacterial Rate Determination of Test Samples

[0056] Note: An inhibition rate of ≥50%~90% indicates antibacterial effect; an inhibition rate of ≥90% indicates strong antibacterial effect.

[0057] Table 5 shows that Sample 1 (egg yolk oil) has a strong antibacterial effect against Staphylococcus aureus and Candida albicans, Sample 2 (flaxseed oil) and Sample 3 (borage seed oil) have antibacterial effects against Staphylococcus aureus and Candida albicans, and the combination of Samples 4-8 has a strong antibacterial effect against Staphylococcus aureus and Candida albicans. This indicates that the antibacterial effect of the combination of egg yolk oil, flaxseed oil, and borage seed oil is better than that of the single components, and has a synergistic effect. Among them, Sample 4 has the highest antibacterial rate, indicating that the antibacterial effect is optimal when the mass ratio of egg yolk oil, flaxseed oil, and borage seed oil is 12:1:1.

[0058] Example 5: Stimulation Test 1. Test Sample Same as Example 1 2. Testing Methods In accordance with the requirements for human skin patch testing in the "Cosmetic Safety Technical Specifications (2015 Edition)," 35 participants were selected based on the inclusion criteria. The participants were aged 18-25 years, including 20 males and 15 females. 20-25 mg of the test sample was placed in a qualified patch applicator and applied to the inside of the participant's arm for 24 hours. Skin reactions were observed and recorded at 30 min, 24 h, and 48 h after removal of the patch applicator, according to the "Skin Reaction Grading Standard for Closed Patch Testing." The skin reaction grading standard for closed patch testing is shown in Table 6, and the experimental results are shown in Table 7.

[0059] Table 6. Grading Criteria for Skin Reactions in Occlusive Patch Tests

[0060] Table 7 Results of human skin patch test

[0061] As shown in Table 7, all 35 subjects showed negative skin reactions to compositions 1-8 at 30 min, 24 h, and 48 h after removal of the spot tester, indicating that the naturally derived compositions of the present invention are mild, non-irritating, and highly safe.

[0062] Example 6: Human Erythema Experiment 1. Test Sample Mix 14 wt% of compositions 1-8 from Table 1 with 86 wt% olive oil to obtain samples 1-8, with sample 0 being olive oil.

[0063] 2. Testing instruments VapoMeter transdermal moisture meter, MoistureMeterSC skin moisture meter, Ultrascan UC22 skin ultrasound analyzer, SkinColorCatch skin color meter.

[0064] 3. Testing Methods Ninety infants aged 0-3 years with diaper rash were selected and divided into 9 groups of 10 each. Samples aged 0-8 were randomly assigned to the 9 groups. Caregivers applied the sample twice daily to the affected area for 7 days. Before and after treatment, various indicators were measured on the same area. Five evenly distributed points were selected in the test area, and the average value was taken. The skin barrier repair effect was characterized by three indicators: skin moisture content change rate measured by a skin moisture meter, transepidermal water loss rate (TEWL) change rate measured by a transepidermal water loss meter, and epidermal thickness change rate measured by a skin ultrasound analyzer. The redness reduction effect was measured using a skin color meter. The value change rate was characterized, and the test results are shown in Table 8. Figure 5 , Figure 6 The formula for the rate of change is: ; In the formula: X — Rate of change, % A0—Initial measurement value; A1 – Measurement value after 7 days.

[0065] Table 8 Human Test Results

[0066] Note: Compared with sample 0. Compared with sample 1, # P <0.05.

[0067] Samples 1-8 had the same total active ingredient content, as shown in Table 8 and Figure 5 , Figure 6 It can be seen that, compared with sample 0 (olive oil), samples 1-3 showed significantly increased skin water content and epidermal thickness, and increased transepidermal water loss rate (TEWL) and... The value decreased significantly, and the difference was statistically significant. This indicates that egg yolk oil, flaxseed oil, and borage seed oil all possess good effects in isolating irritants, repairing the skin barrier, reducing inflammation and redness, and moisturizing. Furthermore, compared to sample 1, samples 4-8 showed further increases in skin water content and epidermal thickness, and their transepidermal water loss rate (TEWL) and... The value decreased further, and the difference was statistically significant. # P The value <0.05 indicates that the natural composition of the present invention is more effective than a single active ingredient in treating contact dermatitis in infants and young children, and has a synergistic effect. Among them, the effect of each indicator of sample 4 is better than that of samples 5-8, indicating that the overall effect is optimal when the mass ratio of egg yolk oil, flaxseed oil, and borage seed oil is 12:1:1.

[0068] As can be seen from the above embodiments, the naturally derived composition provided by the present invention is mild and non-irritating, with high safety. It can synergistically act on the key mechanisms and targets that cause contact dermatitis in infants and young children, effectively preventing and treating contact dermatitis in infants and young children, and maintaining healthy skin. It has excellent effects of isolating irritants, repairing the skin barrier, anti-inflammatory, soothing and reducing redness, antibacterial, and moisturizing. Moreover, it does not contain hormones or antibiotics, is naturally derived, will not produce drug resistance, and can be widely used in cosmetics and topical skin preparations.

[0069] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. Other embodiments can be obtained based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A naturally derived composition, characterized in that, It includes egg yolk oil, flaxseed oil, and borage seed oil; the mass ratio of the egg yolk oil, flaxseed oil, and borage seed oil is (1~50):(0.1~10):(0.1~10).

2. The naturally derived composition according to claim 1, characterized in that, The mass ratio of egg yolk oil, flaxseed oil, and borage seed oil is (5~30):(0.5~5):(0.5~5).

3. The naturally derived composition according to claim 2, characterized in that, The mass ratio of egg yolk oil, flaxseed oil, and borage seed oil is 12:1:

1.

4. The use of the natural source composition according to any one of claims 1 to 3 in the preparation of a product for treating and / or preventing contact dermatitis in infants and young children, wherein the product for treating and / or preventing contact dermatitis in infants and young children includes cosmetics or topical skin preparations.

5. The application according to claim 4, characterized in that, The product for treating and / or preventing contact dermatitis in infants and young children contains the natural source composition at a mass percentage of 1 to 100%.

6. The application according to claim 5, characterized in that, The product for treating and / or preventing contact dermatitis in infants and young children contains the natural source composition at a mass percentage of 5-30%.

7. A cosmetic product, characterized in that, It comprises the natural source composition according to any one of claims 1 to 3.

8. The cosmetic product according to claim 7, characterized in that, The product types of the cosmetics include any one of creams, lotions, serums, toners, masks, gels, jelly, and ointments; the natural source composition in the cosmetics has a mass percentage of 1 to 100%.

9. A topical skin preparation, characterized in that, It comprises the natural source composition according to any one of claims 1 to 3.

10. The topical skin preparation according to claim 9, characterized in that, The product type of the topical skin preparation includes any one of creams, ointments, gels, patches, and sprays; the natural source composition in the topical skin preparation has a mass percentage of 1 to 100%.

Images