A magnetic bead cleaning and extracting mechanism and a magnetic bead cleaning and extracting method

Abstract

The application provides a magnetic bead cleaning and extracting mechanism and a magnetic bead cleaning and extracting method. The mechanism comprises at least a magnetic bead cleaning unit, a magnetic bead extracting unit, a waste liquid collecting unit and a water supply unit. The magnetic bead cleaning unit is connected with the magnetic bead extracting unit, the waste liquid collecting unit and the water supply unit through pipelines. The magnetic bead cleaning unit comprises a magnetic bead cleaning tank, a magnetic component for adsorbing magnetic beads in the cleaning tank and a stirring component for providing stirring in the cleaning tank. The magnetic bead extracting unit is used for collecting and extracting magnetic beads. The waste liquid collecting unit is used for collecting or recycling liquid in the cleaning process. The water supply unit is used for providing cleaning water for the cleaning tank. The technical scheme of the application can automatically complete the cleaning of the magnetic bead mixed liquid and the recycling of the magnetic beads. In the process of producing and preparing the magnetic beads, the efficiency is high and the cost is low.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a magnetic bead cleaning and extraction mechanism and a magnetic bead cleaning and extraction method. Background Technology

[0002] Magnetic beads are a commonly used medium in biological experiments such as nucleic acid extraction, purification, and fragment screening. By mixing magnetic beads with specific nucleic acid binding reagents, the magnetic beads can selectively bind to target nucleic acid molecules. Under appropriate conditions, the nucleic acid bound to the magnetic beads is dissociated and recovered, forming a pure nucleic acid extract.

[0003] Currently, the market demand for nucleic acid extraction reagents continues to grow, resulting in a supply shortage. One of the key reasons for the slow increase in the supply of nucleic acid extraction reagents is the low production efficiency of magnetic beads during the manufacturing process. This is mainly reflected in the need to clean and recover the magnetic beads from the mixed liquid during preparation to obtain clean magnetic beads for market use.

[0004] In existing technologies, the cleaning and recycling of magnetic beads is generally carried out manually. Specifically, small magnets are used to attract the magnetic beads, which are then stirred by an external agitator to clean them. This process is time-consuming and, since the magnetic beads and other materials are placed in an open container during cleaning, the entire process is susceptible to interference and contamination from the external environment or human intervention. This results in low cleaning and recycling efficiency, inconsistent quality of the cleaned magnetic beads, and high costs. Summary of the Invention

[0005] In view of the shortcomings of the prior art described above, the purpose of this invention is to provide a magnetic bead cleaning and extraction mechanism and a magnetic bead cleaning and extraction method to solve the problems in the prior art.

[0006] To achieve the above and other related objectives, the present invention is implemented by including the following technical solutions.

[0007] The present invention provides a first aspect of a magnetic bead cleaning and extraction mechanism, which includes at least a magnetic bead cleaning unit, a magnetic bead extraction unit, a waste liquid collection unit, and a water supply unit, wherein the magnetic bead cleaning unit is connected to the magnetic bead extraction unit, the waste liquid collection unit, and the water supply unit via pipelines.

[0008] The magnetic bead cleaning unit includes a magnetic bead cleaning tank, a magnetic component for adsorbing magnetic beads in the cleaning tank, and a stirring component for providing stirring inside the cleaning tank.

[0009] The magnetic bead extraction unit is used to collect and extract magnetic beads;

[0010] The waste liquid collection unit is used to collect or recycle liquids during the cleaning process;

[0011] The water supply unit is used to provide cleaning water to the cleaning tank.

[0012] In one embodiment, the waste liquid collection unit includes a liquid recovery tank and / or a waste liquid trough.

[0013] In one embodiment, the water supply unit includes a purified water tank, which has an inlet and an outlet. The inlet is connected to a water pipe, and the outlet is connected to a magnetic bead cleaning unit pipe.

[0014] In one embodiment, the magnetic bead cleaning tank is provided with a drain port and a bottom valve at the bottom. The drain port of the magnetic bead cleaning tank is connected to a main drain pipe. The magnetic bead extraction unit and / or waste liquid collection unit are respectively connected to the main drain pipe through drain branch pipes and extraction branch pipes. The drain branch pipes are provided with a seventh valve and an eighth valve, and the extraction branch pipes are provided with a sixth valve. The liquid recovery tank and / or waste liquid tank are respectively connected to the drain branch pipes through secondary branch pipes. The secondary branch pipe corresponding to the liquid recovery tank is provided with a fourth valve, and the secondary branch pipe corresponding to the waste liquid tank is provided with a fifth valve.

[0015] In one embodiment, a nozzle is also connected to the top of the magnetic bead cleaning tank; the nozzle is connected to the outlet pipe of the purified water tank.

[0016] In one embodiment, the bottom of the magnetic bead cleaning tank has a plurality of magnetic sleeves protruding into the tank cavity and closed at the top. The number of magnetic sleeves can be 1, 2, 3, 4, 5, 6, or 7. More preferably, it is 6.

[0017] In one embodiment, the stirring assembly includes a stirring paddle and a stirring motor for acting inside the tank, the stirring paddle and the stirring motor being connected, and the top of the magnetic bead cleaning tank having a through hole through which the stirring paddle passes.

[0018] In one embodiment, the magnetic component includes at least a plurality of magnetic rods and a lifting motor for controlling the raising and lowering of the magnetic rods to provide or eliminate magnetic force on the magnetic beads.

[0019] In one embodiment, the stirring shaft of the impeller is a magnetic sleeve with its lower end closed.

[0020] In one embodiment, magnetic rods and magnetic sleeves are arranged correspondingly. Specifically, the number and position of magnetic rods can correspond one-to-one with magnetic sleeves, and the corresponding magnetic rods and magnetic sleeves are coaxially arranged.

[0021] In one embodiment, the magnetic bead extraction unit includes at least a magnetic barrel for adsorbing and fixing magnetic beads, an extraction feed pipe, and a liquid outlet pipe; the magnetic bead cleaning tank is provided with a cleaning feed inlet and a cleaning discharge outlet.

[0022] The cleaning outlet of the magnetic bead cleaning tank is connected to the magnetic barrel through an extraction feed pipe, and the magnetic barrel is connected to the waste liquid tank through a liquid outlet pipe.

[0023] The extraction feed pipe and the liquid outlet pipe are respectively connected to the extraction branch pipe.

[0024] In one embodiment, the magnetic barrel for adsorbing and fixing magnetic beads includes an inner magnetic field barrel and a magnetic bead extraction bottle, wherein the magnetic bead extraction bottle is fitted inside the inner magnetic field barrel.

[0025] The cleaning outlet of the magnetic bead cleaning tank is connected to the magnetic bead extraction bottle via an extraction feed pipe, and the magnetic bead extraction bottle is connected to the waste liquid tank via a liquid outlet pipe.

[0026] In one embodiment, the magnetic bead cleaning and extraction mechanism further includes a feed tank, the outlet of which is connected to the cleaning inlet pipeline of the magnetic bead cleaning tank, and a first pump body is provided on the pipeline.

[0027] In one embodiment, a level sensor is provided on the top of the purified water tank.

[0028] In one embodiment, a flow meter and a first valve are provided on the pipeline connecting the water outlet and the magnetic bead cleaning unit.

[0029] In one embodiment, the extraction feed pipe is further provided with a second pump body, a second valve, a liquid inlet sensor, and a filter.

[0030] In one embodiment, a third valve is also provided on the liquid outlet pipe.

[0031] In one embodiment, the inlet of the magnetic bead cleaning tank is also connected to the outlet of the purified water tank.

[0032] The present invention provides a second aspect of a method for cleaning and extracting magnetic beads, wherein the magnetic beads are cleaned and extracted using the magnetic bead cleaning and extraction mechanism described above during the production of magnetic beads.

[0033] The beneficial effects of this invention are:

[0034] 1. The magnetic bead cleaning and extraction mechanism of the present invention can automatically complete the cleaning and recycling of magnetic beads throughout the entire process, and realize the one-time large-scale magnetic bead cleaning and extraction, thereby making the production and preparation of magnetic beads highly efficient and low-cost.

[0035] 2. Furthermore, the water supply unit of the magnetic bead cleaning and extraction mechanism of the present invention is connected to other units in the mechanism by pipelines, which avoids the introduction of other cleaning equipment in the prior art and enables the mechanism to clean itself autonomously.

[0036] 3. The container and pipelines of this facility form a closed environment, which can avoid pollution from human or external environment, and the quality of the cleaned and recycled magnetic beads is more stable and uniform. Attached Figure Description

[0037] Figure 1 The diagram shown is a process flow chart of the magnetic bead cleaning method of the present invention.

[0038] Figure 2 The diagram shown is a structural schematic of the magnetic bead cleaning unit in the magnetic bead cleaning mechanism of the present invention.

[0039] Figure 3 The diagram shows the structure of the upper magnetic rod stirring device in the magnetic bead cleaning unit of the present invention.

[0040] Figure 4 The diagram shown is a structural schematic of the magnetic bead cleaning tank in the magnetic bead cleaning unit of the present invention.

[0041] Figure 5 The diagram shown is a structural schematic of the lower magnetic rod device in the magnetic bead cleaning unit of the present invention.

[0042] Figure 6 The diagram shown is a schematic representation of the magnetic bead extraction unit of this invention.

[0043] Figures 1-6 Explanation of the symbols in the attached icons

[0044] 1 Magnetic bead cleaning unit 11 Magnetic components

[0045] 112 First lifting screw 113 First Lifting Bearing Seat 114 First Lifting Motor 115 Lifting Gear Pair

[0046] 116 Agitator Motor

[0047] 117 Stirring Paddle

[0048] 1172 Stirring Shaft

[0049] 1171 Agitator Blade

[0050] 118 Agitator Gear Pair

[0051] 12 Magnetic Bead Cleaning Tank

[0052] 121 Magnetic sleeve

[0053] 123 Tank Bottom Valve

[0054] 124 nozzles

[0055] 125 Clean the feed inlet

[0056] 13 Lower magnetic components

[0057] 131 Second lifting motor 132 Lower Magnetic Rod

[0058] 133 Second lifting screw 134 Second Lifting Bearing Seat 14 Support frame

[0059] 15 Fixing Plate

[0060] 2 Magnetic Bead Extraction Unit 21 Magnetic bead extraction bottle

[0061] 22 Internal Magnetic Field Bucket

[0062] 23 Extraction feed pipe 24. Liquid outlet pipe

[0063] 3 Waste Liquid Collection Unit

[0064] 31 Liquid recovery tank

[0065] 32 Waste liquid tank

[0066] 4 Water supply units

[0067] 5. Feed tank

[0068] 61 Fourth valve

[0069] 62 Fifth Valve

[0070] 63 Flowmeter

[0071] 64 First Valve

[0072] 65 Second pump body

[0073] 66 Second valve

[0074] 67 Filters

[0075] 68 Liquid Inlet Sensor

[0076] 69. Sixth Valve

[0077] 70 Third Valve

[0078] 71 First Pump Body

[0079] 72 Seventh Valve

[0080] 73 Eighth Valve Detailed Implementation

[0081] The following specific examples illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

[0082] Please see Figures 1 to 6 It should be understood that the structures, proportions, sizes, etc., illustrated in the accompanying drawings are merely for illustrative purposes to aid those skilled in the art and to facilitate understanding and reading. They are not intended to limit the scope of the invention and therefore have no substantial technical significance. Any modifications to the structure, changes in proportions, or adjustments to size, without affecting the effectiveness and purpose of the invention, should still fall within the scope of the technical content disclosed in this invention. Furthermore, the terms such as "upper," "lower," "left," "right," "middle," and "one" used in this specification are merely for clarity and not intended to limit the scope of the invention. Changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of the invention's implementation.

[0083] like Figure 1 and Figure 2 As shown, the first aspect of this embodiment provides a specific magnetic bead cleaning and extraction mechanism, which includes at least a magnetic bead cleaning unit 1, a magnetic bead extraction unit 2, a waste liquid collection unit 3, and a water supply unit 4. The magnetic bead cleaning unit 1 is connected to the magnetic bead extraction unit 2, the waste liquid collection unit 3, and the water supply unit 4 through pipelines.

[0084] The magnetic bead cleaning unit 1 includes a magnetic bead cleaning tank 12, a magnetic component for adsorbing magnetic beads in the cleaning tank, and a stirring component for providing stirring in the cleaning tank.

[0085] The magnetic bead extraction unit 2 is used to collect and extract magnetic beads;

[0086] The waste liquid collection unit 3 is used to collect or recycle liquid during the cleaning process;

[0087] The water supply unit 4 is used to provide cleaning water to the cleaning tank.

[0088] In a like Figure 1 In the specific embodiment shown, the waste liquid collection unit 3 includes a liquid recovery tank 31 and a waste liquid tank 32. The liquid recovery tank 31 is used to recover the liquid requiring centralized treatment from the raw material liquid of the magnetic beads to be cleaned and recycled; this liquid is typically ammonia. As an example, in one specific embodiment, the liquid recovery tank 31 is an ammonia recovery tank; the waste liquid tank 32 is used to discharge wastewater from the cleaning process.

[0089] like Figure 1 As shown, in a specific embodiment, the water supply unit 4 includes a purified water tank, which has an inlet and an outlet. The inlet is connected to a water pipe, and the outlet is connected to a pipeline of the magnetic bead cleaning unit 1.

[0090] like Figure 1 and Figure 4 As shown, in a specific embodiment, the magnetic bead cleaning tank 12 is provided with a drain port and a bottom valve 123 at the bottom. The drain port of the magnetic bead cleaning tank 12 is connected to a main drain pipe. The magnetic bead extraction unit 2 and / or the waste liquid collection unit 3 are respectively connected to the main drain pipe through a drain branch pipe and an extraction branch pipe. The drain branch pipe is provided with a seventh valve 72 and an eighth valve 73, and the extraction branch pipe is provided with a sixth valve 69. The liquid recovery tank 31 and / or the waste liquid tank 32 are respectively connected to the drain branch pipe through a secondary branch pipe. The secondary branch pipe corresponding to the liquid recovery tank 31 is provided with a fourth valve 61, and the secondary branch pipe corresponding to the waste liquid tank 32 is provided with a fifth valve 62.

[0091] In actual use, simply add the raw material liquid for cleaning and recycling the magnetic beads to the magnetic bead cleaning unit 1. When the raw material liquid first enters the magnetic bead cleaning unit 1, a magnetic force is applied using a magnetic component, and the magnetic beads are attracted by the magnetic component in the magnetic bead cleaning tank 12. At this time, open the bottom valve 123, the seventh valve 72, the eighth valve 73, and the fourth valve 61, and the liquid in the raw material liquid is recovered and enters the liquid recovery tank 31. Then close the bottom valve 123, the seventh valve 72, the eighth valve 73, and the fourth valve 61. Then, the purified water tank delivers clean water to the magnetic bead cleaning tank 12, removes the magnetic force of the magnetic component, and the magnetic beads detach and fall off. The stirring component in the magnetic bead cleaning tank 12 begins to stir and clean, and the first cleaning of the magnetic beads is completed after a period of time.

[0092] If multiple rounds of cleaning are required, the magnetic component is used again to apply magnetic force. The magnetic beads are attracted by the magnetic component in the magnetic bead cleaning tank 12. The bottom valve 123, the seventh valve 72, the eighth valve 73, and the fifth valve 62 are opened, and the cleaning liquid is recycled into the waste liquid tank 32. The bottom valve 123 and the fifth valve 62 are closed, and the purified water tank supplies clean water to the magnetic bead cleaning tank 12 again. At the same time, the magnetic force of the magnetic component is removed, and the magnetic beads desorb and fall off. The stirring component in the magnetic bead cleaning tank 12 starts stirring to start the next round of cleaning. The above process of magnetic bead adsorption, drainage, addition, desorption, and stirring is repeated many times to complete multiple rounds of cleaning.

[0093] After cleaning, the cleaned magnetic beads mixture is transported to the magnetic bead extraction unit 2 through a pipeline. The cleaned magnetic beads are attracted and collected by magnetic attraction, and the remaining liquid is discharged into the waste liquid tank 32, thus obtaining clean magnetic beads and completing the entire magnetic bead cleaning operation.

[0094] When the magnetic bead cleaning and extraction mechanism needs to be cleaned, close the bottom valve 123 of the tank, open the first pump body 71, and provide the required clean water through the water supply unit 4. The first pump body 71 provides the power to transport water and the flushing force to clean the magnetic bead cleaning and extraction mechanism, thereby realizing the cleaning process of the magnetic bead cleaning and extraction mechanism.

[0095] In one specific embodiment, a level sensor is provided on the top of the purified water tank. For example... Figure 1 As shown, in a specific embodiment, a flow meter 63 and a first valve 64 are provided on the pipeline connecting the outlet of the purified water tank and the magnetic bead cleaning unit 1.

[0096] The water level in the purified water tank is determined by a liquid level sensor to ensure sufficient water supply. The first valve 64 and the flow meter 63 are used to further control whether water enters the magnetic bead cleaning unit 1 and the amount of water entering, based on the actual cleaning situation.

[0097] In a like Figures 2-4 In the specific embodiment shown, the bottom of the magnetic bead cleaning tank 12 has a plurality of magnetic sleeves 121 that protrude into the tank cavity and are closed at the top. As an example, the number of magnetic sleeves 121 can be 1, 2, 3, 4, 5, or 6. More preferably, it is 6.

[0098] In one specific embodiment, the stirring assembly includes a stirring paddle 117 and a stirring motor 116 for acting inside the tank. The stirring paddle 117 and the stirring motor 116 are connected, and the top of the magnetic bead cleaning tank 12 has a through hole through which the stirring paddle 117 passes.

[0099] This application does not specify the connection method between the stirring paddle 117 and the stirring motor 116, as long as it is a method in the prior art that can drive the stirring paddle 117 to rotate through the stirring motor 116.

[0100] As an example, in a... Figure 2 and Figure 3 In the specific embodiment shown, the stirring assembly further includes a stirring gear pair 118; the stirring paddle 117 is meshed with the stirring gear pair 118.

[0101] The stirring motor 116 is connected to the stirring gear pair 118 for transmission. The stirring motor 116 provides power, which drives the stirring paddle 117 to rotate through the stirring gear pair 118 to achieve stirring. In a specific embodiment, the stirring shaft 1172 of the stirring paddle is a magnetic sleeve with its lower end closed.

[0102] In one specific embodiment, the magnetic component includes at least a plurality of magnetic rods and a lifting motor for controlling the raising and lowering of the magnetic rods.

[0103] In one specific embodiment, magnetic rods and magnetic sleeves are correspondingly arranged. Specifically, the number and position of magnetic rods correspond one-to-one with the magnetic sleeves, and the corresponding magnetic rods and magnetic sleeves are coaxially arranged. By controlling the magnetic rods to enter or leave the magnetic sleeves through a lifting motor, the magnetic force of the magnetic beads in the magnetic bead cleaning tank 12 can be provided or eliminated.

[0104] This application does not impose specific limitations on the connection method between the magnetic rod and the motor used to control the lifting and lowering of the magnetic rod, as long as it is a method in the prior art that can drive the magnetic rod to make lifting and lowering movements by means of a motor.

[0105] As an example, the magnetic assembly also includes a lifting screw, a lifting bearing seat, and a lifting gear pair; the lifting screw is connected to the lifting gear pair, and the lifting gear pair is also connected to the lifting motor drive.

[0106] The lifting bearing seat, lifting screw and magnetic rod are connected in sequence, and the lifting screw passes through the lifting bearing seat.

[0107] Specifically, the lifting motor provides power to the lifting gear pair, thereby driving the lifting screw to move up and down through the lifting gear pair 115; the lifting bearing seat ensures that the lifting screw moves in a straight line.

[0108] As an example, in a... Figures 2-5 In the specific embodiment shown, the magnetic assembly can be divided into an upper magnetic assembly 11 and a lower magnetic assembly 13. The magnetic rod in the upper magnetic assembly 11 is coaxially arranged with the stirring shaft 1172, and their shapes and sizes are matched; the magnetic rod in the lower magnetic assembly 13 is coaxially arranged with the magnetic sleeve 121 that is closed at the upper end of the bottom of the tank, and their shapes and sizes are matched.

[0109] More specifically, such as Figure 2 As shown, the upper magnetic assembly 11 includes an upper magnetic rod 111, a first lifting screw 112, a first lifting bearing seat 113, a first lifting motor 114, and a lifting gear pair 115; the first lifting screw 112 is connected to the lifting gear pair 115, and the lifting gear pair is also connected to the first lifting motor 114 in a transmission connection; the first lifting bearing seat 113, the first lifting screw 112, and the upper magnetic rod 111 are connected and arranged sequentially from top to bottom, and the first lifting screw 112 passes through the first lifting bearing seat 113.

[0110] More specifically, such as Figure 5 As shown, the lower magnetic component 13 includes a lower magnetic rod 132, a second lifting screw 133, a second lifting bearing seat 134, and a second lifting motor 131; the lower magnetic rod 132, the second lifting screw 133, and the second straight bearing seat are connected and arranged sequentially from top to bottom, and the second lifting screw 133 passes through the second lifting bearing seat 134.

[0111] The second lifting motor 131 is connected to the second lifting screw 133 through gear transmission, thereby providing power for the up and down movement of the second lifting screw 133 through the second lifting motor 131.

[0112] In actual use, when the raw material liquid for cleaning and recycling magnetic beads enters the magnetic bead cleaning tank 12 in the magnetic bead cleaning unit 1, the magnetic rod in the magnetic component moves up and down via a lifting motor, entering the magnetic sleeve, creating a magnetic attraction in the magnetic bead cleaning tank 12. This attracts the magnetic beads to be cleaned onto the magnetic sleeve 121 and the stirring shaft 1172 at the bottom of the tank. Simultaneously, the liquid in the magnetic bead cleaning tank 12 is discharged through the bottom valve 123. Then, purified water is supplied to the magnetic bead cleaning tank 12 through a purified water tank. The magnetic rod in the magnetic component then moves up and down, leaving the magnetic sleeve, causing the magnetic attraction in the magnetic bead cleaning tank 12 to disappear, and the magnetic beads fall into the tank. The stirring component then begins to stir and clean the magnetic beads. After a period of time, the stirring component stops stirring, completing one cleaning cycle. The adsorption, drainage, addition, desorption, and stirring processes are repeated multiple times until the cleaning of the magnetic beads is complete.

[0113] This application does not specifically limit the connection method of the magnetic bead cleaning tank 12, the magnetic component for cleaning the magnetic beads inside the cleaning tank, and the stirring component for providing agitation inside the cleaning tank. The magnetic component and the stirring component can be integrated on the cleaning tank, or a support device can be provided to achieve a fixed connection between the magnetic bead cleaning tank 12, the magnetic component, and the stirring component.

[0114] As an example, such as Figure 2 As shown, in a specific embodiment, the magnetic bead cleaning unit 1 further includes a support frame 14 and a fixing plate 15, and the magnetic bead cleaning tank 12 is disposed on the support frame 14, thereby providing a space for accommodating and installing the lower magnetic assembly 13;

[0115] The fixing plate 15 is connected and fixed to the support frame 14. The lifting bearing seat of the lower magnetic component 13 is located on the fixing plate 15. The fixing plate 15 is connected and fixed to the support frame 14, thereby fixing the lower magnetic component 13. The lifting bearing seat of the upper magnetic component 11 is located on the top of the magnetic bead cleaning tank 12, thereby fixing the upper magnetic component 11.

[0116] In this application, the number of magnetic rods is several. For example, the number of magnetic rods can be 1, 2, 3, 4, 5, or 6.

[0117] By setting up multiple magnetic rods and magnetic sleeves, it is ensured that all magnetic beads can be adsorbed, thus preventing the magnetic beads from being discharged into the waste liquid collection unit 3.

[0118] In one specific embodiment, the magnetic bead extraction unit 2 includes at least a magnetic bucket for adsorbing and fixing magnetic beads, an extraction feed pipe 23, and a liquid outlet pipe 24; the magnetic bead cleaning tank 12 is provided with a cleaning feed inlet 125 and a cleaning discharge outlet.

[0119] The cleaning outlet of the magnetic bead cleaning tank 12 is connected to the magnetic bucket through the extraction feed pipe 23, and the magnetic bucket is connected to the waste liquid tank 32 through the liquid outlet pipe 24.

[0120] The extraction feed pipe 23 and the liquid outlet pipe 24 are respectively connected to the extraction branch pipe.

[0121] As an example, such as Figure 6 As shown, the magnetic bucket used to adsorb and fix magnetic beads includes an inner magnetic field bucket 22 and a magnetic bead extraction bottle 21, with the magnetic bead extraction bottle 21 fitted inside the inner magnetic field bucket 22; the cleaning outlet of the magnetic bead cleaning tank 12 is connected to the magnetic bead extraction bottle 21 through the extraction feed pipe 23, and the magnetic bead extraction bottle 21 is connected to the waste liquid tank 32 through the liquid outlet pipe 24.

[0122] like Figure 1 As shown, in a specific embodiment, the extraction feed pipe 23 is also provided with a second pump body 65, a second valve 66, a filter 67 and a liquid inlet sensor 68.

[0123] like Figure 1 As shown, in one specific embodiment, a third valve 70 is also provided on the liquid outlet pipe 24.

[0124] After the magnetic beads are cleaned, open the sixth valve 69 and the second valve 66. The second pump 65 will then pump the cleaned magnetic bead mixture into the magnetic bead extraction bottle 21, passing through the inlet sensor 68 and filter 67 before entering. After the magnetic bead mixture enters the magnetic bead extraction bottle 21 through the extraction feed pipe 23, the cleaned magnetic beads are attracted to the inner wall of the bottle due to the magnetic attraction of the inner magnetic field tank 22. At this point, close the sixth valve 69 and open the second pump 65, the third valve 70, and the fifth valve 62 to discharge the remaining liquid into the waste liquid tank 32 through the outlet. The operator can then remove the magnetic bead extraction bottle 21 and retrieve the clean magnetic beads.

[0125] like Figure 1 As shown, in a specific embodiment, the magnetic bead cleaning mechanism further includes a feed tank 5, the outlet of the feed tank 5 is connected to the cleaning inlet 125 of the magnetic bead cleaning tank 12, and a first pump body 71 is provided on the pipeline.

[0126] The first pump body 71 provides power for the feed tank 5 to deliver the mixed liquid of magnetic beads to be cleaned to the magnetic bead cleaning tank 12;

[0127] During the cleaning of the magnetic bead cleaning and extraction mechanism, the water supply unit simultaneously supplies water to and rinses the feed tank 5.

[0128] like Figure 1 As shown, in a specific embodiment, the cleaning inlet 125 of the magnetic bead cleaning tank 12 is also connected to the outlet of the purified water tank.

[0129] like Figure 1 and Figure 4 As shown, in a specific embodiment, a nozzle 124 is also connected to the top of the magnetic bead cleaning tank 12; the nozzle 124 is connected to the outlet pipe of the purified water tank.

[0130] During the cleaning process, when the magnetic rod leaves the magnetic sleeve and the magnetic beads detach, purified water is sprayed through nozzle 124 to wash the magnetic beads off the magnetic sleeve 121, further ensuring that the adsorbed magnetic beads fall off and achieving better stirring and cleaning.

[0131] The present invention provides a second aspect of a method for cleaning and extracting magnetic beads, wherein the magnetic beads are cleaned and extracted using the magnetic bead cleaning and extraction mechanism described above during the production of magnetic beads.

[0132] Specifically, during the cleaning of magnetic beads, after adding the liquid containing the magnetic beads to the feed tank 5, the first pump body 71 is turned on to transport the liquid containing the magnetic beads to the magnetic bead cleaning tank 12. Then, the magnetic rod in the magnetic assembly moves up and down via a lifting motor, entering the magnetic sleeve, creating a magnetic attraction in the magnetic bead cleaning tank 12, thereby attracting the magnetic beads to be cleaned onto the magnetic sleeve 121 and the stirring shaft 1172 at the bottom of the tank. Then, the liquid in the raw material solution of the magnetic bead cleaning tank 12 is discharged through the bottom valve 123. Afterward, the magnetic rod in the magnetic assembly moves up and down, leaving the magnetic sleeve, causing the magnetic attraction in the magnetic bead cleaning tank 12 to disappear, and some magnetic beads fall into the tank. At the same time, purified water is supplied to the magnetic bead cleaning tank 12 through a purified water tank and sprayed through the nozzle 124 until all the magnetic beads fall into the tank. Then, the stirring assembly starts to stir and clean the magnetic beads. After a period of time, the stirring assembly stops stirring, completing one cleaning process for the magnetic beads. The process of adsorption and desorption of magnetic beads, drainage, addition of liquid, desorption and stirring is repeated multiple times until the cleaning of the magnetic beads to be cleaned is completed.

[0133] After cleaning the magnetic beads, open the bottom valve 123, the sixth valve 69, and the second valve 66. The second pump 65 will then pump the cleaned magnetic bead mixture into the magnetic bead extraction bottle 21, passing through the inlet sensor 68 and the filter 67 before entry. Once the magnetic bead mixture enters the extraction bottle 21 via the extraction feed pipe 23, the cleaned magnetic beads are attracted to the inner wall of the bottle due to the magnetic attraction of the inner magnetic field tank 22. At this point, close the sixth valve 69 and open the third valve 70 and the fifth valve 62. The remaining liquid is then pumped through the second pump 65 and discharged into the waste liquid tank 32 through the outlet. The operator can then remove the magnetic bead extraction bottle 21 and retrieve the clean magnetic beads.

[0134] In summary, the magnetic bead cleaning mechanism of the present invention can automatically complete the cleaning of the magnetic bead mixture and the recycling of the magnetic beads throughout the entire process, thereby achieving high efficiency and low cost in the production and preparation of magnetic beads. Moreover, the container and pipeline of the mechanism form a closed environment, which can avoid contamination by human or external environment, and the quality of the cleaned and recycled magnetic beads is more stable and uniform. In addition, the magnetic bead cleaning mechanism itself has a cleaning function, eliminating the need to introduce other cleaning equipment.

[0135] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. A magnetic bead cleaning and extraction mechanism, characterized in that, It includes at least a magnetic bead cleaning unit (1), a magnetic bead extraction unit (2), a waste liquid collection unit (3), and a water supply unit (4). The magnetic bead cleaning unit (1) is connected to the magnetic bead extraction unit (2), the waste liquid collection unit (3), and the water supply unit (4) through pipelines. The magnetic bead cleaning unit (1) includes a magnetic bead cleaning tank (12), a magnetic component for adsorbing magnetic beads in the cleaning tank, and a stirring component for providing stirring in the cleaning tank. The magnetic bead extraction unit (2) is used to collect and extract magnetic beads; The waste liquid collection unit (3) is used to collect or recycle liquid during the cleaning process; The water supply unit (4) is used to provide cleaning water to the cleaning tank.

2. The magnetic bead cleaning and extraction mechanism according to claim 1, characterized in that, The waste liquid collection unit (3) includes a liquid recovery tank (31) and / or a waste liquid tank (32); And / or, the water supply unit (4) includes a purified water tank, which is provided with an inlet and an outlet. The inlet is connected to a water pipe, and the outlet is connected to a pipe of the magnetic bead cleaning unit (1).

3. The magnetic bead cleaning and extraction mechanism according to claim 2, characterized in that, The bottom of the magnetic bead cleaning tank (12) is provided with a drain port and a bottom valve (123). The drain port of the magnetic bead cleaning tank (12) is connected to a main drain pipeline. The magnetic bead extraction unit (2) and / or waste liquid collection unit (3) are respectively connected to the main drain pipeline through the drain branch pipeline and the extraction branch pipeline. The drainage branch pipeline is equipped with a seventh valve (72) and an eighth valve (73), and the extraction branch pipeline is equipped with a sixth valve (69); The liquid recovery tank (31) and / or waste liquid tank (32) are respectively connected to the drain branch pipeline through secondary branch pipelines; a fourth valve (61) is provided on the secondary branch pipeline corresponding to the liquid recovery tank (31); a fifth valve (62) is provided on the secondary branch pipeline corresponding to the waste liquid tank (32); And / or, a nozzle (124) is also connected to the top of the magnetic bead cleaning tank (12); the nozzle (124) is connected to the outlet pipe of the purified water tank.

4. The magnetic bead cleaning and extraction mechanism according to claim 2, characterized in that, The bottom of the magnetic bead cleaning tank (12) has a plurality of magnetic sleeves (121) that protrude into the tank cavity and are closed at the top. And / or, the stirring assembly includes a stirring paddle (117) and a stirring motor (116) for acting inside the tank, the stirring paddle (117) and the stirring motor (116) are connected, and the top of the magnetic bead cleaning tank (12) has a through hole for the stirring paddle (117) to pass through; And / or, the magnetic component includes at least a plurality of magnetic rods and a lifting motor for controlling the raising and lowering of the magnetic rods.

5. The magnetic bead cleaning and extraction mechanism according to claim 4, characterized in that, The stirring shaft (1172) of the stirring paddle (117) is a magnetic sleeve with its lower end closed.

6. The magnetic bead cleaning and extraction mechanism according to claim 5, characterized in that, The magnetic rod and magnetic sleeve are set to correspond.

7. The magnetic bead cleaning and extraction mechanism according to claim 3, characterized in that, The magnetic bead extraction unit (2) includes at least a magnetic barrel for adsorbing and fixing magnetic beads, an extraction feed pipe (23), and a liquid outlet pipe (24); the magnetic bead cleaning tank (12) is provided with a cleaning feed inlet and a cleaning discharge outlet; the cleaning discharge outlet of the magnetic bead cleaning tank (12) is connected to the magnetic barrel through the extraction feed pipe (23), and the magnetic barrel is connected to the waste liquid tank (32) through the liquid outlet pipe (24); The extraction feed pipe (23) and the liquid outlet pipe (24) are respectively connected to the extraction branch pipe.

8. The magnetic bead cleaning and extraction mechanism according to claim 7, characterized in that, The magnetic barrel used for adsorbing and fixing magnetic beads includes an inner magnetic field barrel (22) and a magnetic bead extraction bottle (21), wherein the magnetic bead extraction bottle (21) is fitted inside the inner magnetic field barrel (22); The cleaning outlet of the magnetic bead cleaning tank (12) is connected to the magnetic bead extraction bottle (21) through the extraction feed pipe (23), and the magnetic bead extraction bottle (21) is connected to the waste liquid tank (32) through the liquid outlet pipe (24). And / or, the magnetic bead cleaning mechanism further includes a feed tank (5), the outlet of which is connected to the cleaning inlet pipe of the magnetic bead cleaning tank (12), and a first pump body (71) is provided on the pipe.

9. The magnetic bead cleaning and extraction mechanism according to claim 7, characterized in that, The purified water tank is equipped with a liquid level sensor on its top; And / or, a flow meter (63) and a first valve (64) are provided on the pipeline connecting the outlet of the purified water tank and the magnetic bead cleaning unit (1); And / or, characterized in that, a second pump body (65), a second valve (66), a filter (67) and a liquid inlet sensor (68) are also provided on the extraction feed pipe (23); And / or, a third valve (70) is also provided on the liquid outlet pipe (24); And / or, the cleaning inlet of the magnetic bead cleaning tank (12) is also connected to the outlet of the purified water tank.

10. A method for cleaning and extracting magnetic beads, characterized in that, In the process of producing magnetic beads, the magnetic bead cleaning and extraction mechanism as described in any one of claims 1 to 9 is used to clean and extract the magnetic beads.

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