Environment-sensitive fluorescent probe for mrgx2 and preparation method and application thereof

By designing an environmentally sensitive MrgX2 fluorescent probe, utilizing polyethylene glycol linkers and Nile Red fluorescent chromophores, the problems of low signal-to-noise ratio and non-specific staining in existing probe imaging were solved, achieving stable and specific labeling and high-resolution observation of the MrgX2 receptor.

CN122356458APending Publication Date: 2026-07-10XI AN JIAOTONG UNIV
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Patent Information

Application Number
CN202610489330.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-14
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing MrgX2 fluorescent probes are susceptible to autofluorescence interference in biological samples, resulting in low signal-to-noise ratios in imaging and severe nonspecific membrane staining, making it difficult to achieve stable, specific labeling and high-resolution observation of cell membrane surface receptors.

Method used

A class of environmentally sensitive MrgX2 fluorescent probes was designed, using N-((1s,4s)-4-((4-cyano-3-(trifluoromethyl)phenyl)amino)cyclohexyl)-4-methoxybenzamide as the receptor recognition group, and linking the Nile Red fluorescent chromophore with a polyethylene glycol linker to construct a fluorescent probe with environmentally sensitive properties. The fluorescence emission is located in the red light region and can respond to changes in the microenvironment.

Benefits of technology

Stable and specific fluorescent labeling of the MrgX2 receptor was achieved, reducing background interference, improving the imaging signal-to-noise ratio and detection sensitivity, and enhancing imaging clarity in complex biological systems.

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Abstract

This invention discloses a class of environmentally sensitive MrgX2 fluorescent probes, their preparation methods, and applications, belonging to the field of biomedical and chemical probe technology. Using a small molecule compound with MrgX2 receptor antagonistic activity as a recognition backbone, a long-chain polyethylene glycol linker is introduced by recognizing the active O or N sites on the aromatic ring substituents in the backbone molecule, and then covalently coupled to a Nile Red fluorescent chromophore to obtain the environmentally sensitive MrgX2 fluorescent probe. Compared with existing probes, the fluorescence emission of this probe is located in the red light region, which can reduce the interference of autofluorescence in biological samples and improve the detection signal-to-noise ratio. Simultaneously, the long-chain polyethylene glycol linker improves the probe's hydrophilicity and dispersibility, reducing non-specific membrane staining and background adsorption, which is beneficial for achieving stable and specific fluorescent labeling of the MrgX2 receptor on the cell membrane surface, making it suitable for fluorescent labeling and visualization analysis of the MrgX2 receptor in cell models.
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