White color cultivable bamboo bird's nest and its application

Abstract

This invention discloses a creamy-white culturable bamboo bird's nest and its applications, belonging to the field of biotechnology. The culturable bamboo bird's nest of this invention is named Wenxue (BBN2) and was deposited at the China General Microbiological Culture Collection Center (CGMCC) on September 10, 2025, with the accession number CGMCC NO.42098. This invention is based on a culturable bamboo bird's nest strain, “Green Coral” (BBN1). During artificial subculturing, the fruiting body tissues with variant traits were isolated multiple times, ultimately yielding the culturable creamy-white bamboo bird's nest strain of this invention. Compared to wild bamboo bird's nest, the culturable bamboo bird's nest of this invention is creamy-white in color, with transparent fruiting body branches, no black or other discoloration at the base, and a wider growth temperature range than wild bamboo bird's nest. The culturable creamy-white bamboo bird's nest of this invention can be produced on a large scale artificially, and also exhibits high disease resistance, showing better market prospects.

Description

[0001] This application is a divisional application of patent application No. 202511655260.7, filed on November 12, 2025. Technical Field

[0002] This invention relates to the field of biotechnology, specifically to a creamy-white cultivable bamboo bird's nest and its applications. Background Technology

[0003] Bamboo bird's nest, scientifically known as *Scorias spongiosa*, also called bamboo fungus, bamboo flower, or bamboo mushroom, is a valuable fungal food. It originates from the secretions left on bamboo by the bamboo aphid after it feeds on the sap of young bamboo. Under suitable temperature and humidity, these secretions are utilized by microorganisms, forming a natural fungal-microbial symbiosis. Because its appearance and taste are similar to bird's nest, with a fresh, smooth texture, it was named bamboo bird's nest. Bamboo bird's nest contains various essential and non-essential amino acids, proteins, vitamins, and polysaccharides, among other active substances. It possesses antibacterial, anti-inflammatory, and antioxidant properties, and is therefore widely used in the production and processing of nutritional and health products. As a novel resource, bamboo bird's nest has enormous economic value and development potential. However, current research indicates that bamboo bird's nest cannot form large fruiting bodies, and the methods for obtaining it on the market rely entirely on wild collection, making large-scale artificial production and cultivation difficult. Therefore, cultivating a type of cultivable bamboo bird's nest that is both aesthetically pleasing and disease-resistant is beneficial for opening up new bamboo bird's nest markets, thereby realizing the commercial development of bamboo bird's nest products. Summary of the Invention

[0004] In order to overcome the above-mentioned shortcomings of the existing technology, the purpose of this invention is to provide a creamy white culturable bamboo bird's nest and its application, so as to provide a culturable bamboo bird's nest with stronger disease resistance, stable inheritance and use for commercial cultivation and breeding of bamboo bird's nest, and solve the problems of weak disease resistance and narrow growth temperature range of existing bamboo bird's nest.

[0005] The technical solution of this invention to solve the above-mentioned technical problems is as follows: A creamy-white cultivable bamboo bird's nest is provided. This cultivable bamboo bird's nest is named "Wenxue" and was deposited on September 10, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. Its accession number is CGMCC NO.42098, and its classification name is *Spongeia spongioides* (CGMCC genus). Scorias spongiosa ).

[0006] This invention provides the application of the above-mentioned off-white culturable bamboo bird's nest in the preparation of food or health products.

[0007] The present invention provides a food product, including the above-mentioned off-white cultivable bamboo bird's nest.

[0008] This invention provides a health product, including the above-mentioned off-white cultivable bamboo bird's nest.

[0009] This invention provides an application of the above-mentioned off-white cultivable bamboo bird's nest in the agricultural cultivation of bamboo bird's nest.

[0010] This invention provides a method for cultivating bamboo bird's nest in agriculture, comprising the following steps: placing the young fruiting bodies of the cultivable bamboo bird's nest strain on a raised, arc-shaped plastic culture rack, controlling the ambient temperature at 20-23℃, the air humidity at 90-95%, the carbon dioxide concentration at less than 1000ppm, and cultivating in the dark, spraying nutrient solution every 3 hours, and cultivating for 13-15 days.

[0011] Furthermore, the nutrient solution includes the following components: glucose, honey, sugar, and water.

[0012] Furthermore, the mass ratio of glucose, honey, white sugar, and water is 15-20:1-5:1-5:70-75.

[0013] Furthermore, the mass ratio of glucose, honey, white sugar, and water is 20:2:3:75.

[0014] This invention offers the following advantages: Based on a culturable bamboo bird's nest strain "Wenxue" (BBN1), the invention, through multiple isolations of fruiting body tissues exhibiting variant traits during artificial subculturing, ultimately yielded the culturable off-white bamboo bird's nest strain of this invention. Compared to wild bamboo bird's nest, the culturable bamboo bird's nest of this invention is off-white in color, with a crystal-clear cell structure, and exhibits a wider growth temperature range (10-25℃). Furthermore, the culturable off-white bamboo bird's nest of this invention possesses high disease resistance, giving it a better market prospect. Attached Figure Description

[0015] Figure 1 Image of the off-white culturable bamboo bird's nest "Wenxue" of this invention;

[0016] Figure 2 Image showing the disease resistance of bamboo bird's nest with "snow-covered" patterns. Detailed Implementation

[0017] The examples given below are for illustrative purposes only and are not intended to limit the scope of the invention. Unless otherwise specified, conditions in the examples are performed under standard conditions or as recommended by the manufacturer. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0018] Example

[0019] A method for isolating and artificially cultivating off-white cultivable bamboo bird's nests, specifically including the following steps:

[0020] (1) Separation of off-white cultivated bamboo bird's nest

[0021] 1. Preparation of PDA plate culture medium: Weigh 200g of peeled potatoes and cut them into strips. Put them in a pot, add 1L of deionized water, and then cook the potato strips until soft. After filtering, pour the filtrate into a beaker, add deionized water to make up the volume of the filtrate to 1L, add 20g of glucose and 18g of agar powder, heat until completely dissolved, and pour the culture medium into a petri dish (15mL of culture medium) while hot. Let it cool and solidify to obtain the culture medium.

[0022] 2. During the artificial subculture process, the fruiting body tissue of the bamboo bird's nest "Green Coral" (BBN1, a variety isolated by the inventor from wild bamboo bird's nest in the early stage, which was deposited at the China General Microbiological Culture Collection Center on September 10, 2025, with the accession number CGMCC NO.42097) with color variation was cut with a sterile scalpel and inoculated into the PDA plate medium prepared in step 1. It was then placed in a constant temperature incubator at 25℃ for 5 days. After the fruiting body formed, the stability of the variation was observed. The color variation tissue was cut again until a stable off-white bamboo bird's nest strain with stable genetic traits was obtained.

[0023] (2) Artificial cultivation of off-white bamboo bird's nest

[0024] The young fruiting bodies of the off-white bamboo bird's nest strain obtained in step (1) were placed on a raised arc-shaped plastic culture rack. The temperature was controlled at 23°C, the air humidity at 95%, the carbon dioxide concentration at 500ppm, and the environment was kept dark. A specially prepared nutrient solution was sprayed every 3 hours, and the culture was carried out for 13-15 days.

[0025] By comparing the growth rate and color of the fruiting bodies, a milky-white bamboo bird's nest strain named "Wenxue" (see [link to strain name]) was selected. This strain exhibits excellent comprehensive traits, including rapid growth, high disease resistance, and no discoloration at the base, and possesses stable heritable characteristics. Figure 1 ).

[0026] The culturable off-white bamboo bird's nest strain of this invention, "Wenxue," has an artificial cultivation growth cycle of 13-15 days. Compared to wild bamboo bird's nests, which grow only once a year depending on the season and coexist with bamboo and aphids, the bamboo bird's nest strain of this invention can achieve artificial cultivation growth about 24 times a year. Under the same cultivation environment, its growth cycle is reduced by at least 3 days compared to the wild domesticated strain "Green Coral," resulting in faster growth and lower cultivation costs.

[0027] The cultivable off-white bamboo bird's nest of this invention, "Wenxue," is entirely off-white without any other colors, while wild bamboo bird's nests are mostly pale yellow. The white color of the fungus is unique and stable, possessing high value for genetic research and variety breeding.

[0028] The culturable off-white bamboo bird's nest variety "Wenxue" of this invention exhibits disease resistance during its growth process. Figure 2As shown. During the artificial cultivation of bamboo bird's nests, high humidity and high nutrient conditions easily lead to black rot at the base of the fruiting body. However, under the same storage conditions and growth conditions, the "Snowflake" bamboo bird's nest variety basically does not suffer from this disease, while the incidence rate of wild-caught domesticated bamboo bird's nest variety "Green Coral" can reach 30%. Figure 2 A represents the off-white, cultivable bamboo bird's nest of this invention. Figure 2 B represents the state of wild-caught, domesticated bamboo bird's nest, known as "green coral." Figure 2 It is known that the base of the cultivable off-white bamboo bird's nest of the present invention does not develop black lesions during the growth process, therefore the cultivable bamboo bird's nest of the present invention has good disease resistance.

[0029] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for cultivating bamboo bird's nests in agriculture, characterized in that, The process includes the following steps: inoculate the fruiting bodies of the cultivable bamboo bird's nest strain onto a culture rack, control the temperature at 20-23℃, the air humidity at 90-95%, the carbon dioxide concentration at less than 1000ppm, culture in the dark, spray nutrient solution every 3 hours, and culture for 13-15 days.

2. The method for cultivating bamboo bird's nests according to claim 1, characterized in that, The off-white culturable bamboo bird's nest was named Wenxue and was deposited at the China General Microbiological Culture Collection Center on September 10, 2025, with the accession number CGMCC NO.42098.

3. The method for cultivating bamboo bird's nests according to any one of claims 1-2, characterized in that, The nutrient solution comprises the following components: glucose, honey, sugar, and water.

4. The method for cultivating bamboo bird's nest according to any one of claims 1-3, characterized in that, The mass ratio of glucose, honey, white sugar and water is 15-20:1-5:1-5:70-75.

5. The method for cultivating bamboo avian nests according to any one of claims 1-4, characterized in that, The mass ratio of glucose, honey, white sugar and water is 20:2:3:75.

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