Full-automatic fluorescent staining microscopic image scanning and analyzing system

Abstract

This invention relates to the field of medical device technology and discloses a fully automated fluorescence staining microscopic image scanning and analysis system, including a fully automated fluorescence staining imager. A coverslip compartment is fixedly connected to the top outer wall of the fully automated fluorescence staining imager for storing coverslips. A cover-applying module is provided on the top of the fully automated fluorescence staining imager. Utilizing the curvature formed by the coverslip during slide removal, combined with a rotating pressing action, when the curved coverslip moves above the slide and presses down, the lowest point of its curved surface preferentially forms point contact with the sample to be tested. As the rotating frame continues to rotate, the coverslip is gradually pressed and flattened from the contact point to both sides, allowing the sample to be tested to be evenly spread radially outward from the center, effectively shortening the air escape path and avoiding air bubble residue. Simultaneously, the internal elastic potential energy of the coverslip is released smoothly, preventing the coverslip from breaking due to localized stress concentration, thereby improving the sealing quality.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, specifically to a fully automated fluorescence staining microscopic image scanning and analysis system. Background Technology

[0002] Immunofluorescence detection technology is a core technology in the field of clinical microbiology testing with high sensitivity and specificity. It plays an irreplaceable role in the rapid identification of pathogenic microorganisms, early diagnosis of infectious diseases, and monitoring of treatment efficacy. It has been widely used in the detection and diagnosis of various pathogens such as fungi, vaginal microecology evaluation, Mycobacterium tuberculosis, respiratory pathogens, and viruses, providing key evidence for precise clinical diagnosis and treatment.

[0003] Traditional immunofluorescence assays rely heavily on manual operation. The complete process involves multiple consecutive steps, including sample smearing, fixation, staining, incubation, mounting, and fluorescence microscopy. The operation is cumbersome and time-consuming, requiring a high level of proficiency and standardization from the testing personnel. Furthermore, human differences can easily lead to problems such as uneven staining quality, result interpretation bias, and insufficient experimental repeatability.

[0004] While some automated staining equipment has emerged on the market, most are designed for single detection items or specific pathogens. These devices lack versatility and adaptability to various scenarios, making it difficult to simultaneously meet the immunofluorescence detection needs of different types of organisms, such as fungi, vaginal microecology, and Mycobacterium tuberculosis. Furthermore, the existing equipment has limited automation and integration levels, with gaps remaining in sample transport, reagent addition, incubation temperature control, multi-step washing, waste disposal, and pre-microscope pretreatment. These processes still require significant manual assistance and intervention, making it difficult to meet the high-throughput, standardized, and regulated testing needs of clinical practice.

[0005] In summary, during automated coverslip preparation, the extremely thin and smooth coverslips are prone to sticking together due to static electricity and intermolecular forces when stacked. This causes frequent abnormalities such as multiple coverslips being picked up at once or failure to pick up coverslips during equipment aspiration, leading to equipment downtime or poor sealing. Furthermore, when coverslips are placed on slides containing samples to be tested, air bubbles are easily trapped, interfering with microscopic observation, causing rework of slides, and ultimately affecting the accuracy of pathological diagnosis.

[0006] To address this, we propose a fully automated fluorescence staining microscopic image scanning and analysis system. Summary of the Invention

[0007] To address the shortcomings of existing technologies, this invention provides a fully automated fluorescence staining microscopic image scanning and analysis system. During slide retrieval, after the suction cup adsorbs the top coverslip, an inflatable airbag pushes the middle area of ​​the coverslip, creating a controllable arc-shaped bend. Elastic stress breaks the capillary adhesion between the coverslips, forming tiny gaps. Simultaneously, some gas enters the gas outlet chamber through a tube, forming a high-speed airflow that blows into the gaps. Through the separation force of the airflow and the electrostatic neutralization of ionized air, the lower coverslips are reliably separated, ensuring that only one coverslip is picked up at a time. During slide placement, the coverslip, maintaining a preset arc, is moved above the slide. Rotation and pressing ensure that the lowest point of the arc surface preferentially contacts the sample to be tested. Subsequently, it is gradually flattened from the center outwards. This process allows the sample to extend evenly outwards from the contact point, effectively expelling air and eliminating air bubbles. Simultaneously, it gently releases the internal stress of the coverslip, avoiding the risk of breakage, thereby improving the sealing quality and operational stability, and solving the problems mentioned in the background art.

[0008] To achieve the above objectives, the present invention provides the following technical solution: The fully automated fluorescence staining microscopic image scanning and analysis system includes a fully automated fluorescence staining imager. The fully automated fluorescence staining imager is internally equipped with electrical components, test tube components, reagent components, cleaning components, single-well slide components, double-well slide components, imaging analysis components, and waste slide and waste liquid treatment components. The fully automated fluorescence staining imager is internally equipped with a coverslip compartment for storing coverslips. A cover-applying module is provided on the side of the fully automated fluorescence staining imager near the coverslip compartment.

[0009] In the above technical solution, the cover module includes a fixing frame, which is fixedly connected inside the fully automatic fluorescence staining imaging instrument. A drive motor is fixedly installed on the outer wall of the fixing frame near the cover glass compartment. The output end of the drive motor is fixedly connected to a drive roller. An auxiliary roller is connected to the end of the fixing frame away from the drive roller via a rotating shaft. A belt is wound around the outer surface of the drive roller and the auxiliary roller. A sliding frame is fixedly connected to the outer surface of the belt. A first auxiliary motor is fixedly connected to the top outer wall of the sliding frame. A rotating shaft is fixedly connected to the output end of the first auxiliary motor. An adjustment component for picking up and placing cover glass slides is provided at the end of the rotating shaft.

[0010] Preferably, the adjustment component includes a sliding block, which is slidably sleeved on the outer surface of the rotating shaft. A connecting frame is fixedly connected to the outer surface of the sliding block, and a sliding groove is formed on the outer surface of the connecting frame. A second auxiliary motor is fixedly connected to the outer surface of the connecting frame near the sliding block. The output end of the second auxiliary motor is fixedly connected to the rotating frame. The first auxiliary motor drives the adjustment component to rise and fall vertically through the rotating shaft, thereby adjusting the vertical distance between the adjustment component and the coverslip compartment when picking up the coverslip, and the vertical distance between the adjustment component and the slide when placing the coverslip, so as to meet the height and posture adjustment requirements of the coverslip in different work positions such as picking, transferring, and placing.

[0011] Furthermore, an auxiliary wheel is mounted on the outer surface of the rotating frame near the slide groove via a rotating shaft. The auxiliary wheel is rolled on the inner wall of the slide groove, and the slide groove is used to limit the deflection angle of the rotating frame via the auxiliary wheel.

[0012] Based on the above, a first connecting tube is fixedly connected to the outer wall of the rotating frame away from the auxiliary wheel. The end of the first connecting tube passes through the rotating frame and is fixedly connected to the suction cup. The top of the suction cup is fixedly connected to the rotating frame. When the suction cup picks up the cover glass, the suction cup contacts the upper surface of the cover glass and draws air from the suction cup through the first connecting tube to generate negative pressure to adsorb and fix the cover glass.

[0013] Correspondingly, a fixing tube is also fixedly connected to the outer wall of the rotating frame near the first connecting tube. An air bladder is fixedly connected to the end of the fixing tube. The air bladder is used to push the middle area of ​​the cover glass outward when it is inflated, so that a gap is formed between the adhered cover glass sheets, which facilitates subsequent separation.

[0014] It is worth noting that an elastic rope is fixedly connected inside the airbag. The end of the elastic rope away from the airbag is fixedly connected to the rotating frame. When the airbag deflates, the elastic rope provides a restoring force, causing the airbag to contract and return to its original position towards the bottom of the rotating frame. The elastic rope ensures that the airbag can stably and repeatedly complete the ejection action, avoiding the attenuation of ejection force caused by residual deformation of the airbag during operation.

[0015] Meanwhile, a second connecting tube is fixedly connected to the outer wall of the fixed tube near the airbag. An outlet chamber is fixedly connected to the end of the second connecting tube away from the fixed tube. A one-way valve is installed inside the end of the second connecting tube near the outlet chamber. The one-way valve limits the airflow to flow only from the fixed tube to the outlet chamber, preventing gas backflow. When the fixed tube inflates the airbag, some gas enters the outlet chamber through the second connecting tube and is ejected towards the coverslip. Simultaneously, because the outlet nozzle of the outlet chamber is flat, it is aimed at the gap created by the airbag bending during the coverslip's movement, spraying a high-speed airflow. After the airbag completes its bending action, the side wall of the airbag can seal the outlet of the outlet chamber, preventing interference with the sample on the slide when the coverslip is placed on the slide.

[0016] Based on the above, when the fixed tube injects gas into the airbag, part of the gas enters the air outlet chamber through the second connecting tube, and the air outlet chamber discharges the gas toward the cover glass side. The airflow released by the air outlet chamber blows toward the gap between the two adhered cover glass sheets, and the separation force generated by the airflow separates the cover glass sheet located on the lower side from the adsorbed cover glass sheet, so as to ensure that only one cover glass sheet is picked up at a time.

[0017] Compared with existing technologies, it has the following beneficial effects: 1. In this fully automated fluorescence staining microscopic image scanning and analysis system, the curved arc formed by the coverslip during the slide picking process, combined with the rotation and pressing action, ensures that when the curved coverslip is moved above the slide and pressed down, the lowest point of its curved surface preferentially forms point contact with the sample to be tested. As the rotating frame continues to rotate, the coverslip is gradually pressed and flattened from the contact point to both sides, so that the sample to be tested is evenly spread radially from the center outward, effectively shortening the air venting path and avoiding air bubble residue. At the same time, the elastic potential energy inside the coverslip is released smoothly, which can prevent the coverslip from breaking due to local stress concentration, thereby improving the sealing quality. 2. In this fully automated fluorescence staining microscopic image scanning and analysis system, the expansion of an air bladder causes the top coverslip to bend in a controllable arc, creating a tiny initial gap between adjacent adhered coverslips using elastic stress. Simultaneously, a portion of the gas is introduced into the exhaust chamber via a second connecting tube, forming a directional airflow aligned with this gap. This airflow reliably completes the separation and pickup of individual coverslips without generating stress that could cause the coverslips to break, thus effectively improving the stability and yield of the slide retrieval process. Attached Figure Description

[0018] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings: Figure 1 This is a schematic diagram of the fully automated fluorescence staining imaging system of the present invention; Figure 2 This is a schematic diagram of the internal structure of the fully automated fluorescence staining imaging instrument of the present invention; Figure 3 This is a schematic diagram of the cover module structure of the present invention; Figure 4 This is a schematic diagram of the adjustment component structure of the present invention; Figure 5 This is a schematic diagram of the exploded structure of the regulating component of the present invention; Figure 6 This is a schematic cross-sectional view of the adjustment component of the present invention. Figure 1 ; Figure 7 This is a schematic cross-sectional view of the adjustment component of the present invention. Figure 2 ; Figure 8 This is a schematic diagram of the structure of the adjustment component of the present invention when it adsorbs the cover glass. Figure 9 This is a schematic diagram of the structure of the airbag of the present invention when it compresses the cover glass. Figure 10 This is a schematic diagram of the structure when the cover glass of the present invention is placed.

[0019] The meanings of the labels in the diagram are as follows: In the diagram: 1. Fully automatic fluorescence staining imaging instrument; 2. Covering module; 201. Fixing frame; 202. Drive motor; 2021. Drive roller; 2022. Auxiliary roller; 203. Belt; 204. Sliding frame; 205. First auxiliary motor; 206. Rotating shaft; 207. Adjustment component; 2071. Sliding block; 2072. Connecting frame; 2073. Slide groove; 2074. Second auxiliary motor; 2075. Auxiliary wheel; 2076. Rotating frame; 2 077. First connecting tube; 2078. Suction cup; 2079. Fixing tube; 20710. Airbag; 20711. Second connecting tube; 20712. One-way valve; 20713. Air outlet chamber; 20714. Elastic rope; 3. Coverslip; 4. Electrical assembly; 5. Test tube assembly; 6. Reagent assembly; 7. Cleaning assembly; 8. Single-well slide assembly; 9. Double-well slide assembly; 10. Imaging analysis component; 11. Coverslip compartment; 12. Waste slide and waste liquid treatment component. Detailed Implementation

[0020] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0021] Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the invention, and should not be construed as limiting the invention.

[0022] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0023] In automated cover glass operations, cover glass slides are thin and have smooth surfaces, making them prone to sticking due to static electricity and intermolecular forces when stacked. This can lead to frequent single-slide failures and multiple slides being picked up at once during the equipment's pick-up process, resulting in equipment downtime or defects in the quality of the cover glass.

[0024] Therefore, in view of the above-mentioned problems, the present invention provides a fully automated fluorescence staining microscopic image scanning and analysis system, with reference to... Figure 1 As shown, the fully automated fluorescence staining imaging instrument 1 includes an electrical component 4, a test tube component 5, a reagent component 6, a cleaning component 7, a single-well slide component 8, a double-well slide component 9, an imaging analysis component 10, and a waste slide and waste liquid treatment component 12. The instrument also includes a coverslip compartment 11 for storing coverslips 3. A cover module 2 is located on the side of the instrument near the coverslip compartment 11. When a sample needs to be scanned under a microscope, the fully automated fluorescence staining imaging instrument 1 is started, and the electrical component 4 controls the operation of each component. First, the test tube component 5 transports the test tube containing the sample to be tested to the working position. The swab application component picks up a sample swab from the test tube and, according to the detection type, evenly and thinly applies the sample to the sample area of ​​the slide provided by the single-well slide component 8 or the double-well slide component 9. Subsequently, the single-well slide assembly 8 or the double-well slide assembly 9 transfers the slide to the liquid addition position, and the reagent assembly 6 and the cleaning assembly 7 add the staining solution, destaining solution and cleaning solution according to the preset program to complete the staining treatment.

[0025] After staining, the slide is moved to the vicinity of the coverslip compartment 11, and the capping module 2 begins the sealing operation. After the capping is completed, the slide is sent to the imaging analysis unit 10 for automatic scanning imaging and result analysis. Finally, the waste slide and waste liquid treatment unit 12 recovers the waste slides and waste liquid, and the test tube unit 5 ejects the empty test tube holder, awaiting the next cycle. (Reference) Figure 2-4 As shown, the cover module 2 includes a fixing frame 201, which is fixedly connected inside the fully automatic fluorescence staining imaging instrument 1. A drive motor 202 is fixedly installed on the outer wall of the fixing frame 201 near the cover plate compartment 11. The output end of the drive motor 202 is fixedly connected to the drive roller 2021. An auxiliary roller 2022 is connected to the end of the fixing frame 201 away from the drive roller 2021 through a rotating shaft. A belt 203 is wound around the outer surface of the drive roller 2021 and the auxiliary roller 2022. A sliding frame 204 is fixedly connected to the outer surface of the belt 203. The drive motor 202 drives the sliding frame 204 to reciprocate in the horizontal direction through the belt 203, thereby realizing the transfer of the cover plate 3 between the cover plate compartment 11 and the slide preparation station.

[0026] refer to Figure 5-6 As shown, a first auxiliary motor 205 is fixedly connected to the top outer wall of the sliding frame 204. A rotating shaft 206 is fixedly connected to the output end of the first auxiliary motor 205. An adjustment component 207 for picking up and placing coverslips 3 is provided at the end of the rotating shaft 206. It is worth noting that the adjustment component 207 includes a sliding block 2071, which is slidably sleeved on the outer surface of the rotating shaft 206. A connecting frame 2072 is fixedly connected to the outer surface of the sliding block 2071. A second auxiliary motor 2074 is fixedly connected to the outer surface of the connecting frame 2072 near the sliding block 2071. The output end of the second auxiliary motor 2074 is fixedly connected to the rotating frame 2076. The first auxiliary motor 205 drives the adjustment component 207 to rise and fall vertically as a whole through the rotating shaft 206, thereby adjusting the vertical distance between the adjustment component 207 and the coverslip compartment 11 when picking up the coverslip 3, and the vertical distance between the adjustment component 207 and the slide when placing the coverslip 3, so as to meet the height and posture adjustment requirements of the coverslip 3 in different work positions such as picking up, transferring and placing.

[0027] refer to Figure 3-4 As shown, the outer surface of the connecting frame 2072 is provided with a sliding groove 2073. The outer surface of the rotating frame 2076 near the sliding groove 2073 is equipped with an auxiliary wheel 2075 via a rotating shaft. The auxiliary wheel 2075 is rolled on the inner wall of the sliding groove 2073. The sliding groove 2073 is used to limit the deflection angle of the rotating frame 2076 via the auxiliary wheel 2075.

[0028] A first connecting pipe 2077 is fixedly connected to the outer wall of the rotating frame 2076 away from the auxiliary wheel 2075. The end of the first connecting pipe 2077 passes through the rotating frame 2076 and is fixedly connected to the suction cup 2078. The top of the suction cup 2078 is fixedly connected to the rotating frame 2076. The adjustment component 207 is set above the cover glass compartment 11. The first auxiliary motor 205 drives the adjustment component 207 to descend to the picking height. The suction cup 2078 contacts the uppermost cover glass 3 and forms a negative pressure adsorption.

[0029] refer to Figure 7-8 As shown, a fixing tube 2079 is also fixedly connected to the outer wall of the rotating frame 2076 near the first connecting tube 2077. An airbag 20710 is fixedly connected to the end of the fixing tube 2079. The airbag 20710 is made of highly elastic rubber and possesses good flexibility and fatigue resistance. When the airbag 20710 inflates... Figure 7 As shown in F1), an outward pushing force is applied to the middle area of ​​the coverslip 3, causing the uppermost coverslip 3, which is adsorbed by the suction cup 2078, to form a controllable arc-shaped bending deformation, thus presenting a shape that is low in the middle and high on both sides. When the airbag 20710 is completely pushed out ( Figure 9 As shown in F3), the elastic stress generated by the bending of the coverslip 3 can effectively break the capillary adhesion between the coverslips 3, forming micro gaps between adjacent adhered coverslips 3. At the same time, the bending shape of the coverslip 3 also provides conditions for subsequent placement.

[0030] An elastic rope 20714 is fixedly connected inside the airbag 20710. The end of the elastic rope 20714 away from the airbag 20710 is fixedly connected to the rotating frame 2076. When the airbag 20710 deflates, the elastic rope 20714 provides a restoring force, causing the airbag 20710 to retract and reset towards the bottom of the rotating frame 2076. The elastic rope 20714 ensures that the airbag 20710 can stably and repeatedly complete the ejection action, avoiding the airbag 20710 from experiencing residual deformation during operation, which would cause the ejection force to decrease.

[0031] A second connecting pipe 20711 is fixedly connected to the outer wall of the fixed pipe 2079 near the airbag 20710. An air outlet chamber 20713 is fixedly connected to the end of the second connecting pipe 20711 away from the fixed pipe 2079. A one-way valve 20712 is provided inside the end of the second connecting pipe 20711 near the air outlet chamber 20713. The one-way valve 20712 is used to limit the airflow to flow only from the fixed pipe 2079 to the air outlet chamber 20713 to prevent gas backflow. When the fixed tube 2079 inflates the airbag 20710, some of the gas enters the gas outlet chamber 20713 through the second connecting tube 20711 and is sprayed out towards the coverslip 3. At the same time, since the gas outlet of the gas outlet chamber 20713 is set to a flat shape, it sprays a high-speed airflow into the gap created by the coverslip 3 during the bending process of the airbag 20710. After the airbag 20710 completes the bending action, the side wall of the airbag 20710 can block the gas outlet of the gas outlet chamber 20713, so as to prevent the gas outlet of the gas outlet chamber 20713 from interfering with the sample on the slide when the coverslip 3 is placed on the slide.

[0032] When electrostatic adsorption and capillary adhesion exist between the coverslips 3, the existing technology uses vibration separation. However, vibration separation increases the risk of breakage of the coverslips 3. Therefore, some gas is introduced into the gas outlet chamber 20713 through the second connecting pipe 20711. Figure 7 As shown in F2), the gas ejected from the exhaust chamber 20713 can be ionized air, ordinary compressed air, or inert gas. The ionized air is generated by an ion generator and contains a large number of positive and negative ions. When blown onto the surface of the coverslip 3, it can neutralize the static charge generated by friction and eliminate the electrostatic field, thereby reducing the influence of electrostatic adsorption during subsequent slide retrieval. At the same time, the airflow can remove micro-dust from the surface of the coverslip 3, keeping its lower surface clean and preventing particles from being trapped and causing artificial artifacts, thus improving the quality of slides. The gas is discharged from the exhaust chamber 20713 towards one side of the coverslip 3. The airflow released by the exhaust chamber 20713 blows towards the gap between two adhered coverslips 3. The separation force generated by the airflow separates the lower coverslip 3 from the adsorbed coverslip 3, ensuring that only one coverslip 3 is picked up at a time. The bending deformation creates an initial gap between the adhered coverslips 3, providing a channel for airflow to enter. After the high-speed airflow enters the gap, it can destroy the gas-liquid interface of capillary adhesion and generate axial thrust to assist separation. At the same time, this separation method can avoid the risk of the coverslip 3 breaking due to local stress concentration. The airflow acts directly on the adhesion interface and has the effect of breaking electrostatic adsorption and capillary adhesion.

[0033] refer to Figure 9As shown, when placing the coverslip 3, the airbag 20710 pushes out to maintain the coverslip 3 at a preset curvature. After moving it above the slide, the first auxiliary motor 205 drives the adjustment assembly 207 to descend to the set height, and the second auxiliary motor 2074 drives the rotating frame 2076 to rotate slowly, so that the coverslip 3 contacts the sample to be tested with the lowest point of the curvature. As the rotating frame 2076 continues to rotate ( Figure 10 As shown in D1), the coverslip 3 is gradually pressed flat from the lowest point to both sides until it completely adheres to the slide ( Figure 10 As shown in D2, it is worth noting that the coverslip 3 forms point contact with the slide at its lowest point. The sample to be tested extends evenly radially outward from the contact point, resulting in a shorter and more uniform air expulsion path, which can effectively prevent air bubble residue. Furthermore, during the process of the coverslip 3 being fitted from an arc shape to a flat surface, the contact area gradually expands outward from the center, ensuring that the sample to be tested is spread evenly. The internal stress of the coverslip 3 is released gradually, avoiding breakage due to local stress concentration. At the same time, the bending deformation during slide removal is restored during the slide placement and flattening process, and the elastic potential energy is released gradually and converted into the power for uniform pressing.

[0034] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0035] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A fully automated fluorescence staining microscopic image scanning and analysis system, comprising a fully automated fluorescence staining imager (1), wherein the fully automated fluorescence staining imager (1) is internally provided with an electrical component (4), a test tube component (5), a reagent component (6), a cleaning component (7), a single-well slide component (8), a double-well slide component (9), an imaging analysis component (10), and a waste slide and waste liquid treatment component (12), wherein the fully automated fluorescence staining imager (1) is internally provided with a coverslip compartment (11), the coverslip compartment (11) being used to store coverslips (3), characterized in that, The fully automated fluorescence staining imaging instrument (1) has a cover module (2) on the side near the cover plate compartment (11). The cover module (2) includes: A fixed frame (201) is fixedly connected inside the fully automatic fluorescence staining imaging instrument (1). A drive motor (202) is fixedly installed on the outer wall of the fixed frame (201) near the coverslip compartment (11). The output end of the drive motor (202) is fixedly connected to the drive roller (2021). An auxiliary roller (2022) is connected to the end of the fixed frame (201) away from the drive roller (2021) through a rotating shaft. A belt (203) is wound around the outer surface of the drive roller (2021) and the auxiliary roller (2022). A sliding frame (204) is fixedly connected to the outer surface of the belt (203). A first auxiliary motor (205) is fixedly connected to the top outer wall of the sliding frame (204). A rotating shaft (206) is fixedly connected to the output end of the first auxiliary motor (205). An adjustment component (207) for picking up and placing coverslips (3) is provided at the end of the rotating shaft (206).

2. The fully automated fluorescence staining microscopic image scanning and analysis system according to claim 1, characterized in that: The adjustment component (207) includes a sliding block (2071), which is slidably sleeved on the outer surface of the rotating shaft (206). A connecting frame (2072) is fixedly connected to the outer surface of the sliding block (2071). A sliding groove (2073) is provided on the outer surface of the connecting frame (2072). A second auxiliary motor (2074) is fixedly connected to the outer surface of the connecting frame (2072) near the sliding block (2071). The output end of the second auxiliary motor (2074) is fixedly connected to the rotating frame (2076).

3. The fully automated fluorescence staining microscopic image scanning and analysis system according to claim 2, characterized in that: An auxiliary wheel (2075) is mounted on the outer surface of the rotating frame (2076) near the slide groove (2073) via a rotating shaft. The auxiliary wheel (2075) is rolled on the inner wall of the slide groove (2073). The slide groove (2073) is used to limit the deflection angle of the rotating frame (2076) via the auxiliary wheel (2075).

4. The fully automated fluorescence staining microscopic image scanning and analysis system according to claim 2, characterized in that: A first connecting tube (2077) is fixedly connected to the outer wall of the rotating frame (2076) away from the auxiliary wheel (2075). The end of the first connecting tube (2077) passes through the rotating frame (2076) and is fixedly connected to the suction cup (2078). The top of the suction cup (2078) is fixedly connected to the rotating frame (2076). When the suction cup (2078) picks up the cover glass (3), the suction cup (2078) contacts the upper surface of the cover glass (3) and draws air from the suction cup (2078) through the first connecting tube (2077) to generate negative pressure to adsorb and fix the cover glass (3).

5. The fully automated fluorescence staining microscopic image scanning and analysis system according to claim 2, characterized in that: The rotating frame (2076) is also fixedly connected to a fixing tube (2079) on the outer wall of the side near the first connecting tube (2077). An airbag (20710) is fixedly connected to the end of the fixing tube (2079). The airbag (20710) is used to push the middle area of ​​the cover glass (3) outward when it is inflated, so that a gap is formed between the adhered cover glass (3), which is convenient for subsequent separation.

6. The fully automated fluorescence staining microscopic image scanning and analysis system according to claim 5, characterized in that: An elastic rope (20714) is fixedly connected inside the airbag (20710). The end of the elastic rope (20714) away from the airbag (20710) is fixedly connected to the rotating frame (2076). When the airbag (20710) deflates, the elastic rope (20714) provides a reset force, causing the airbag (20710) to retract and reset towards the bottom of the rotating frame (2076).

7. The fully automated fluorescence staining microscopic image scanning and analysis system according to claim 5, characterized in that: The fixed tube (2079) is fixedly connected to the outer wall of the side near the airbag (20710) by a second connecting tube (20711), and the end of the second connecting tube (20711) away from the fixed tube (2079) is fixedly connected to an air outlet chamber (20713).

8. The fully automated fluorescence staining microscopic image scanning and analysis system according to claim 7, characterized in that: The second connecting pipe (20711) has a one-way valve (20712) inside the end near the air outlet chamber (20713). The one-way valve (20712) is used to restrict the flow direction of airflow in the second connecting pipe (20711).

9. The fully automated fluorescence staining microscopic image scanning and analysis system according to claim 8, characterized in that: When the fixed tube (2079) injects gas into the airbag (20710), part of the gas enters the air outlet chamber (20713) through the second connecting tube (20711), and the air outlet chamber (20713) discharges the gas toward the cover glass (3).

10. The fully automated fluorescence staining microscopic image scanning and analysis system according to claim 9, characterized in that: The airflow released by the air outlet chamber (20713) blows towards the gap between the two adhered cover glass sheets (3), and the separation force generated by the airflow separates the cover glass sheet (3) located on the lower side from the adsorbed cover glass sheet (3), so as to ensure that only one cover glass sheet (3) is picked up at a time.

Images