Engineered adenosine kinase variants

By engineering adenosine kinase and introducing specific amino acid sequence substitutions, the incompatibility problem in the enzyme cascade reaction was solved, the conversion efficiency of nucleoside substrates was improved, and the homeostasis of intracellular nucleotide levels was maintained.

CN122374445APending Publication Date: 2026-07-10CODEXIS INC
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Patent Information

Application Number
CN202480078779.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2024-06-18
Filing Date
2024-10-11
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing adenosine kinases have incompatibility issues in enzyme cascade reactions, affecting reaction efficiency and stability, and making it difficult to effectively maintain nucleotide levels and concentration gradients across cell membranes.

Method used

By engineering adenosine kinase and introducing substitutions or sets of substitutions for specific amino acid sequences, its properties can be improved to meet the requirements of enzyme cascade reactions, thereby enhancing its efficiency in converting nucleoside substrates into nucleoside monophosphates.

Benefits of technology

It improves the properties of adenosine kinase, increases the efficiency of nucleoside substrate conversion to nucleoside monophosphate, solves the incompatibility problem in enzyme cascade reactions, and maintains the homeostasis of intracellular nucleotide levels.

✦ Generated by Eureka AI based on patent content.

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Abstract

This disclosure provides engineered adenosine kinase peptides and recombinant polynucleotides encoding the engineered adenosine kinase. This disclosure also provides methods for using the engineered adenosine kinase.
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Citation Information

Patent Citations

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    US5763594A