A method for generating biofilm under an applied voltage

Abstract

The present application belongs to the field of wastewater biological treatment and microbial electrochemistry technology, and particularly relates to a method for optimizing the ratio of living cells to dead cells and metabolic activity in the electrode surface biofilm by regulating the applied voltage in an inhibitory environment containing toxic substance 5-hydroxymethylfurfural. The present application significantly improves the ratio of living cells in the biofilm and promotes the secretion of functional extracellular polymeric substance by applying a direct current voltage of 0.8-1.4V in an anaerobic electro-fermentation system, thereby enhancing the system's resistance to toxicity. Experiments show that under a voltage of 1.2V, the ratio of living cells in the biofilm can reach more than 85%, the fluorescence intensity of protein-like and humic acid-like substances in the extracellular polymeric substance is the strongest, and the methane production is the highest and the system startup time is the shortest. The present application optimizes the biofilm structure and metabolic function by voltage regulation, thereby providing a systematic solution for the enhanced treatment and resource recovery of inhibitory wastewater.

Images