A method for isolating and culturing mycoplasma bovigenitalium

By optimizing the culture medium composition and isolation and culture methods, the instability problem of bovine reproductive tract mycoplasma isolation and culture was solved, achieving efficient and stable isolation and culture, improving the growth rate and culture density of bovine reproductive tract mycoplasma, reducing costs and improving experimental efficiency.

CN122405433APending Publication Date: 2026-07-17JINYUBAOLING BIO PHARMA CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JINYUBAOLING BIO PHARMA CO LTD
Filing Date
2026-04-27
Publication Date
2026-07-17

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Abstract

本发明涉及微生物培养技术领域，更具体而言，涉及一种牛生殖道支原体的分离及培养方法。样品采取自牧场奶牛阴道外阴疱疹，采集奶牛阴道拭子；将阴道拭子置于液体培养基中，进行静置培养；培养后进行PCR及16s测序，结果为牛生殖道支原体，同时进行传代及平皿纯化，经过5代培养后进行PCR及16s测序，结果为牛生殖道支原体。制备牛生殖道支原体培养基；接种；静置培养。通过采用包含特定比例的PPLO、MEM、酵母浸粉及丙酮酸钠等成分的优化培养基，为牛生殖道支原体提供了全面且适配的营养环境，使其能够快速适应并启动增殖，显著缩短了生长周期。
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