A compound microbial agent with corn and soybean stalk degradation ability under low temperature conditions and a preparation method and application thereof

The compound inoculant of Trichoderma FS5 and Pseudomonas aeruginosa BNT4 was used to achieve efficient degradation of corn and soybean straw under low temperature conditions, solving the problem of straw resource utilization in Northeast China and improving soil fertility and microbial community richness.

CN122405463APending Publication Date: 2026-07-17RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Filing Date
2026-03-20
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing microbial agents are difficult to effectively degrade corn and soybean straw under low temperature conditions, especially corn and soybean straw in Northeast China, which limits the utilization of straw resources. Moreover, existing research focuses on the degradation of single crop straw and lacks microbial agents that can degrade corn and soybean straw with a broad spectrum.

Method used

A compound microbial agent composed of Trichoderma FS5 and Pseudomonas aeruginosa BNT4 is used. The mixture is cultured and mixed in a fermentation medium to form a synergistic system of "fungal attack + bacterial metabolism". This system can break through the structural barrier of corn straw and rapidly decompose soybean straw protein, achieving efficient degradation at low temperature.

Benefits of technology

At a low temperature of 4℃, the compound microbial agent achieved a degradation rate of 33.12% for corn straw and 8.78% for soybean straw. It also improved soil organic matter by adjusting the carbon-nitrogen ratio and promoted the richness of soil microbial communities, thus solving the problem of straw degradation under low temperature conditions.

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Abstract

本发明公开了一种低温条件下兼具玉米与大豆秸秆降解能力的复合菌剂及其制备方法应用。所述的复合菌剂由保藏编号为CGMCC No.42446的木霉菌(Trichoderma sp.)FS5以及保藏编号为CGMCC No.37567的铜绿假单胞菌(Pseudomonas chlororaphis subsp.)BNT4组成。其中木霉菌FS5能将纤维素分解成较短的纤维寡糖或纤维二糖,铜绿假单胞菌BNT4则可以利用这些中间产物作为碳源,从而避免了这些产物积累对木霉菌FS5产生反馈抑制,间接促进了整个降解过程的效率,从而形成一套高效率的秸秆降解“流水线”。本发明提供的复合菌剂为东北地区主粮作物秸秆的绿色有效处理提供了新的解决方案,对于促进土壤固碳固氮,提高土地肥力及实现农业可持续发展具有重要意义。
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