Behavior observation device for zebra fish tumor model

Abstract

The utility model relates to the technical field of zebra fish tumor experiments, in particular to a zebra fish tumor model behavior observation device which comprises a box body and a transplantation body for zebra fish tumor transplantation, and a containing opening for containing the transplantation body is formed in the upper side of the box body. According to the utility model, water can be replenished to other zebrafish waiting for operation in the tumor transplantation process in a mode of promoting water to flow back into the transplantation body again, and the zebrafish does not need to be transferred after being transplanted, so that the water-changing transfer number of the zebrafish is effectively reduced, and the operation efficiency is improved. According to the scheme, the zebra fish is subjected to water change transfer, namely, scare caused by water change transfer of the zebra fish is reduced to a certain extent, and behavior observation can be performed on the zebra fish after tumor transplantation subsequently through the arrangement of the high-precision camera, so that the influence of tumors on the zebra fish can be controlled, and therefore, the scheme not only can assist personnel in performing tumor transplantation on the zebra fish conveniently, but also can improve the survival rate of the zebra fish. The zebra fish behavior observation effect can be achieved after tumor transplantation is completed.

Description

Technical Field

[0001] The utility model relates to the technical field of zebrafish tumor experiments, in particular to a zebrafish tumor model behavior observation device. Background Art

[0002] Zebrafish are often used in tumor experiments, and the existing Chinese patent with application number: 201820864996.4 discloses a special device for zebrafish larvae tumor transplantation, including a box body and a transplant body; the box body and the transplant body are both rectangular concave bodies with baffles arranged on all sides, the transplant body is located in the concave body of the box body, and the inner bottom surface of the transplant body is provided with at least two rectangular grooves, and the bottom of each rectangular groove is provided with at least two circular drainage holes and a ruler, and at least two groups of scale lines are provided on the inner longitudinal wall of each rectangular groove; four rectangular columns are provided at the four corners of the outer bottom surface of the transplant body.

[0003] Although the above technology has the effect of being used for tumor transplantation in zebrafish, it is impossible to replenish water into the transplanted body after the tumor transplantation, so the zebrafish needs to be transferred to other breeding boxes for breeding and behavioral observation after the zebrafish tumor transplantation. Zebrafish are easily frightened after multiple transfers and water changes, which affects the activity of zebrafish. Therefore, in order to solve the above technical problems, a zebrafish tumor model behavior observation device is provided. Utility Model Content

[0004] The utility model aims to solve the shortcomings in the prior art and proposes a zebrafish tumor model behavior observation device.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A zebrafish tumor model behavior observation device comprises a box body and a transplant for zebrafish tumor transplantation, wherein the upper side of the box body is provided with a receiving port for accommodating the transplant, the bottom of the box body is provided with a liquid storage cavity and a matching piston plate is provided in the liquid storage cavity through a linkage rod, the middle part of the box body is provided with a matching flow groove corresponding to the liquid storage cavity, the flow groove is provided with a plurality of branch grooves corresponding to the receiving port, the box body is provided with matching truncation columns corresponding to the branch grooves, the rear side of the box body is provided with a mounting seat, and the upper surface of the mounting seat is provided with a camera.

[0007] Preferably, a plurality of partition protrusions are provided on the inner bottom surface of the accommodating opening, the upper surfaces of the partition protrusions are in contact with the lower surface of the transplant body via sealing strips, and each branch groove is located between adjacent partition protrusions.

[0008] Preferably, one end of the liquid storage cavity penetrates to the outside of the box body, and a mounting seat is provided at one end of the box body located at the opening of the liquid storage cavity, and the connecting rod is connected to the mounting seat through a thread.

[0009] Preferably, a liquid outlet pipe is provided at one end of the box body away from the opening of the liquid storage cavity, one end of the liquid outlet pipe is connected to the liquid storage cavity, and a sealing cap is installed at the other end of the liquid outlet pipe.

[0010] Preferably, the mounting seat is connected to the box body by screws, and the end of the linkage rod is rotationally connected to the piston plate.

[0011] Preferably, the outer annular surface of the truncation column is connected to the box body through a thread, and a hand wheel is provided at one end of the truncation column located outside the box body.

[0012] Preferably, the box body is a transparent body wall, and a matching contact surface is provided on the upper surface of the box body corresponding to the transplant body.

[0013] The utility model proposes a zebrafish tumor model behavior observation device, which has the following beneficial effects:

[0014] 1: The utility model promotes the water to flow back into the transplanted body, which can not only replenish water for the remaining zebrafish waiting for surgery during the tumor transplantation process, but also eliminates the need to change water and transport the zebrafish after transplantation, thereby effectively reducing the number of water changes and transfers of the zebrafish, that is, reducing the fright of the zebrafish caused by water changes and transfers to a certain extent. Moreover, through the setting of a high-precision camera, the behavior of the zebrafish after the tumor transplantation can be observed in the future, so as to control the impact of the tumor on the zebrafish. That is, the present solution can not only facilitate the assistants to transplant the tumor to the zebrafish, but also have the effect of observing the behavior of the zebrafish after the tumor transplantation is completed, so as to facilitate the personnel to record the experimental data, and through the setting of the separation protrusion, the zebrafish in the transplanted body can be replenished with water in a targeted manner.

[0015] 2: The setting of the partition protrusion in the utility model divides the accommodating port into several independent spaces, and the partition protrusion corresponds to the groove in the transplant body, that is, through the setting of this independent space, personnel can replenish water in a targeted manner when replenishing water to the zebrafish; the setting of the mounting seat can not only improve the stability of the connecting rod, but also make it easier for personnel to push the piston plate to move through the connecting rod, thereby facilitating the forward or reverse flow of the water; the setting of the liquid outlet pipe makes it convenient to discharge the excess water through the liquid outlet pipe when there is too much water inside the box body, and this setting can effectively save the time and energy consumed by personnel when disassembling the transplant body, that is, make this solution more convenient to use; the setting of the screws makes it easy to disassemble the mounting seat, that is, to disassemble the connecting rod and the piston plate, so that when the sealing between the piston plate and the liquid storage chamber decreases after long-term use, it can be maintained in time. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1This is a schematic diagram of a one-view perspective of a zebrafish tumor model behavior observation device proposed in the utility model;

[0017] Figure 2 This is a schematic diagram from another perspective of a zebrafish tumor model behavior observation device proposed by the utility model;

[0018] Figure 3 This is a schematic cross-sectional view of a box of a zebrafish tumor model behavior observation device proposed by the utility model;

[0019] Figure 4 A zebrafish tumor model behavior observation device proposed in the utility model Figure 3 Axonometric diagram of

[0020] Figure 5 A schematic diagram of a branch slot of a zebrafish tumor model behavior observation device proposed in the utility model;

[0021] Figure 6 This is a schematic diagram of the coordination of the branch slots and truncation columns of a zebrafish tumor model behavior observation device proposed by the utility model.

[0022] In the figure: 1. transplant body; 2. box body; 3. liquid storage chamber; 4. connecting rod; 5. piston plate; 6. branch groove; 7. truncation column; 8. camera; 9. partition protrusion; 10. mounting seat; 11. liquid outlet tube; 12. contact surface. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0024] Example 1

[0025] Reference Figure 1-6 A zebrafish tumor model behavior observation device comprises a box body 2 and a transplant 1 for zebrafish tumor transplantation, a receiving port for receiving the transplant 1 is arranged on the upper side of the box body 2, a liquid storage chamber 3 is arranged at the bottom of the box body 2, and a matching piston plate 5 is arranged in the liquid storage chamber 3 through a connecting rod 4, a matching flow groove is arranged in the middle of the box body 2 corresponding to the liquid storage chamber 3, a plurality of branch grooves 6 are arranged in the flow groove corresponding to the receiving port, a matching truncation column 7 is installed in the box body 2 corresponding to the branch groove 6, a mounting seat is arranged at the rear side of the box body 2, and a camera 8 is arranged on the upper surface of the mounting seat.

[0026] Example 2

[0027] Reference Figure 1-6 , when other parts are the same as those in Example 1, the difference between this embodiment and Example 1 is that:

[0028] A plurality of partition protrusions 9 are provided on the bottom surface of the receiving port, and the upper surface of the partition protrusions 9 is abutted against the lower surface of the transplant body 1 through a sealing strip, and each branch groove 6 is respectively located between adjacent partition protrusions 9. The arrangement of the partition protrusions 9 divides the receiving port into a plurality of independent spaces, and the partition protrusions 9 correspond to the grooves in the transplant body 1, that is, through the arrangement of the independent spaces, personnel can replenish water in a targeted manner when replenishing water to the zebrafish; one end of the liquid storage chamber 3 penetrates to the outside of the box body 2, and the end of the box body 2 located at the opening of the liquid storage chamber 3 is provided with a mounting seat 10, and the linkage rod 4 is connected to the mounting seat 10 through a thread, and the mounting seat The setting of 10 can not only improve the stability of the connecting rod 4, but also make it easier for personnel to push the piston plate 5 to move through the connecting rod 4, thereby facilitating the forward or reverse flow of the water; a liquid outlet pipe 11 is provided at one end of the box body 2 away from the opening of the liquid storage chamber 3, one end of the liquid outlet pipe 11 is connected to the liquid storage chamber 3, and a sealing cap is installed at the other end of the liquid outlet pipe 11. The setting of the liquid outlet pipe 11 makes it easy to discharge the excess water through the liquid outlet pipe 11 when there is too much water in the box body 2. This setting can effectively save the time and energy consumed by personnel when disassembling the transplant body 1, and make this solution more convenient to use.

[0029] The mounting seat 10 is connected to the box body 2 by screws, and the end of the connecting rod 4 and the piston plate 5 are rotatably connected. The setting of the screws makes it easy to disassemble the mounting seat 10, that is, to disassemble the connecting rod 4 and the piston plate 5, so that when the sealing between the piston plate 5 and the liquid storage chamber 3 decreases after long-term use, it can be maintained in time; the outer annular surface of the truncation column 7 is connected to the box body 2 by a thread, and a handwheel is provided at one end of the truncation column 7 located outside the box body 2. Since the truncation column 7 and the box body 2 are threadedly connected, the truncation column 7 can maintain a stable connection relationship with the branch groove 6; the box body 2 is a transparent body wall, and the upper surface of the box body 2 is provided with a matching contact surface 12 corresponding to the transplant body 1. The setting of the contact surface 12 makes the transplant body 1 and the box body 2 more tightly combined.

[0030] Principle and advantages of use: During the use of the utility model, when the zebrafish needs to undergo tumor transplantation, the transplant body 1 is used to assist the personnel in performing the tumor transplantation. During this process, the water carried by the zebrafish flows into the box body 2 through the transplant body 1, and further water enters the flow groove through the branch groove 6 on the bottom surface of the receiving port, and finally enters the liquid storage chamber 3. During the zebrafish tumor transplantation process, if the personnel are clumsy in operation or other accidents cause the zebrafish tumor transplantation process to take a long time, at this time, the piston plate 5 can be pushed to move by the connecting rod 4, so that the water inside the liquid storage chamber 3 is squeezed into the box body 2 again, and finally flows back into the transplant body 1 to replenish water for the zebrafish, and then the piston plate 5 is pulled by the connecting rod 4 to move toward the opening of the liquid storage chamber 3, so that the water in the transplant body 1 flows back into the box body 2, and the zebrafish can continue the experiment. Therefore, under the above operation, the phenomenon that the zebrafish is dehydrated and its activity is affected by long operation time can be reduced to a certain extent.

[0031] Furthermore, in the above process, after the zebrafish tumor transplantation is completed, the piston plate 5 is pushed to allow the water to flow back into the transplant body 1. By causing the water to flow back into the transplant body 1, not only can the remaining zebrafish waiting for surgery be replenished with water during the tumor transplantation process, but the zebrafish do not need to be transferred by water change after transplantation, thereby effectively reducing the number of water changes and transfers of the zebrafish, that is, reducing the fright of the zebrafish caused by water changes and transfers to a certain extent. In addition, through the setting of the high-precision camera 8, the behavior of the zebrafish after the tumor transplantation can be observed in the future, so as to control the impact of the tumor on the zebrafish.

[0032] It should be further explained that the setting of the partition protrusion 9 divides the accommodating port into a plurality of independent spaces, and the partition protrusion 9 corresponds to the groove in the transplant body 1, that is, through the setting of this independent space, the personnel can replenish water in a targeted manner when replenishing water to the zebrafish; the setting of the mounting seat 10 can not only improve the stability of the linkage rod 4, but also make it easier for the personnel to push the piston plate 5 to move through the linkage rod 4, thereby facilitating the forward or reverse flow of the water; the setting of the liquid outlet pipe 11 makes it easy to discharge the excess water through the liquid outlet pipe 11 when there is too much water in the box body 2 , and this arrangement can effectively save the time and energy consumed by personnel in disassembling the transplant body 1, that is, make this solution more convenient to use; the arrangement of the screws facilitates the disassembly of the mounting seat 10, that is, the disassembly of the linkage rod 4 and the piston plate 5, so that when the sealing between the piston plate 5 and the liquid storage chamber 3 decreases after long-term use, it can be maintained in time; since the truncation column 7 and the box body 2 are threadedly connected, the truncation column 7 can maintain a stable connection relationship with the branch groove 6; the arrangement of the contact surface 12 makes the combination between the transplant body 1 and the box body 2 tighter.

[0033] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A zebrafish tumor model behavior observation device, comprising a box body (2) and a transplant (1) for zebrafish tumor transplantation, It is characterized in that The upper side of the box body (2) is provided with a receiving opening for receiving the transplant body (1); the bottom of the box body (2) is provided with a liquid storage chamber (3) and a matching piston plate (5) is provided in the liquid storage chamber (3) via a linkage rod (4); a matching flow groove is provided in the middle of the box body (2) corresponding to the liquid storage chamber (3); a plurality of branch grooves (6) are provided in the flow groove corresponding to the receiving opening; matching truncation columns (7) are installed in the box body (2) corresponding to the branch grooves (6); a mounting seat is provided at the rear side of the box body (2); a camera (8) is provided on the upper surface of the mounting seat.

2. A zebrafish tumor model behavior observation device according to claim 1, It is characterized in that The inner bottom surface of the receiving opening is provided with a plurality of partition protrusions (9), the upper surfaces of the partition protrusions (9) abut against the lower surface of the transplant body (1) via sealing strips, and each branch groove (6) is located between adjacent partition protrusions (9).

3. A zebrafish tumor model behavior observation device according to claim 1, It is characterized in that One end of the liquid storage chamber (3) penetrates to the outside of the box body (2); one end of the box body (2) located at the opening of the liquid storage chamber (3) is provided with a mounting seat (10); and the linkage rod (4) is connected to the mounting seat (10) via a thread.

4. A zebrafish tumor model behavior observation device according to claim 3, It is characterized in that A liquid outlet pipe (11) is provided at one end of the box body (2) away from the opening of the liquid storage chamber (3); one end of the liquid outlet pipe (11) is connected to the liquid storage chamber (3); and a sealing cap is installed at the other end of the liquid outlet pipe (11).

5. A zebrafish tumor model behavior observation device according to claim 3, It is characterized in that The mounting seat (10) is connected to the box body (2) via screws, and the end of the linkage rod (4) and the piston plate (5) are rotationally connected.

6. The zebrafish tumor model behavior observation device according to claim 1, It is characterized in that The outer annular surface of the truncation column (7) is connected to the box body (2) via a thread, and a hand wheel is provided at one end of the truncation column (7) located outside the box body (2).

7. The zebrafish tumor model behavior observation device according to claim 1, It is characterized in that The box body (2) is a transparent body wall, and a matching contact surface (12) is provided on the upper surface of the box body (2) corresponding to the transplant body (1).

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