Portable testicular tissue sampling and freezing device

Abstract

The utility model provides a portable testicular tissue sampling and freezing device, and belongs to the field of medical instruments. The sampling and freezing device comprises a sampling component, a freezing component and a freezing component, wherein the sampling component is used for sampling testicular tissues to be treated; the processing assembly is used for washing and fixing the testicular tissues sampled by the sampling assembly; the cooling assembly is used for cooling the testicular tissue treated by the treatment assembly; the freezing assembly is used for cryopreserving the testicular tissue cooled by the cooling assembly, and the sampling assembly, the processing assembly, the cooling assembly and the freezing assembly are all stored in a portable transfer box. According to the portable testicular tissue sampling and freezing device, the problem of emergency fertility preservation of soldiers can be solved, the device is portable, testicular tissue samples of rescued objects can be rapidly collected in time, and the testicular tissues can be rapidly frozen and stored for fertility preservation.

Description

Technical Field

[0001] The utility model belongs to the field of medical devices, and particularly relates to a portable testicular tissue sampling and freezing device. Background Art

[0002] The testis is one of the most important organs in the male reproductive system. Testicular tissue contains mature sperm, immature sperm at all levels, and other germ cells. In reproductive medicine, doctors can obtain testicular tissue through methods such as testicular puncture and microsurgery, select available sperm from it, and enable some infertile men to have offspring through the ICSI technique. This technique is currently widely used in many reproductive medicine centers around the world and is a safe and mature assisted reproductive medicine technique. However, the battlefield environment is complex, and the operability of conventional puncture and surgery in such an environment is low. Moreover, the collection time, treatment methods, treatment means, and preservation methods of testicular tissue from injured or sacrificed officers and soldiers will greatly affect the success rate of subsequent treatment. Therefore, rapid sampling and cryopreservation of testicular tissue are one of the most critical factors.

[0003] Existing testicular tissue sampling techniques mainly include puncture sampling or surgical incision sampling, and cryopreservation techniques are mainly slow-cooling freezing techniques. The above operations need to be carried out in the operating room and laboratory, and the whole process involves consumables and large equipment such as surgical packs, programmable freezers, and liquid nitrogen tanks. Therefore, for the emergency fertility preservation of wounded soldiers, there are problems of timeliness and site equipment. Summary of the Utility Model

[0004] The present utility model is made to solve the above problems, and aims to provide a portable testicular tissue sampling and freezing device.

[0005] The present utility model provides a portable testicular tissue sampling and freezing device, having the following features: including a sampling component for sampling the testicular tissue to be processed; a processing component for washing and fixing the testicular tissue taken by the sampling component; a cooling component for cooling the testicular tissue processed by the processing component; and a freezing component for freezing the testicular tissue cooled by the cooling component. Among them, the sampling component, the processing component, the cooling component, and the freezing component are all stored in a portable transport box.

[0006] In the portable testicular tissue sampling and freezing device provided by the present utility model, it may also have the following features: wherein, the transport box includes: a transport main body, which is a box-shaped structure with an openable cover on one side; a handle, which is stretchably arranged on the transport main body; a plurality of pulleys, which are arranged at the bottom of the transport main body; and 4 storage cabinets, which are arranged inside the transport main body and are respectively used for correspondingly placing the sampling component, the processing component, the cooling component, and the freezing component.

[0007] In the portable testicular tissue sampling and freezing device provided by the present utility model, it may further have the following features: Among them, the sampling assembly includes a sterile ophthalmic scissors, forceps, a puncture needle package, povidone iodine, sterile gauze, and a syringe, and the five are placed separately.

[0008] In the portable testicular tissue sampling and freezing device provided by the present utility model, it may further have the following features: Among them, the processing assembly includes: a multi-well culture plate and a physiological saline storage bottle, which are used by the operator to wash the testicular tissue taken by the sampling assembly; and a freezing tube containing a cell cryoprotectant solution and a multi-well tube rack matching the freezing tube, which are used as the storage environment for the washed testicular tissue.

[0009] In the portable testicular tissue sampling and freezing device provided by the present utility model, it may further have the following features: Among them, the temperature reduction assembly includes: a temperature reduction cup, which is a cup-shaped structure with an upward opening and is used to hold a low-temperature liquid; a temperature reduction cover, which is detachably arranged at the cup mouth of the temperature reduction cup; a first connecting rod, which is columnar and is arranged vertically on the lower bottom surface of the temperature reduction cover, and when the temperature reduction cover is fixed at the cup mouth of the temperature reduction cup, the first connecting rod is located inside the cup body of the temperature reduction cup; and a fixing clamp, which is arranged on the first connecting rod, and the fixing clamp is a clamp-shaped structure with shrinkage elasticity and can clamp the freezing tube in a matching manner.

[0010] In the portable testicular tissue sampling and freezing device provided by the present utility model, it may further have the following features: Among them, the temperature reduction assembly further includes an anti-falling element, and the anti-falling element is a filter screen or a perforated plate matching the inner diameter of the temperature reduction cup. The anti-falling element is arranged in the middle section inside the cup body of the temperature reduction cup, and the liquid level of the low-temperature liquid in the temperature reduction cup is lower than the position where the anti-falling element is located. The positions of the first connecting rod, the fixing clamp, and the freezing tube fixed on the fixing clamp are all higher than the position where the anti-falling element is located.

[0011] In the portable testicular tissue sampling and freezing device provided by the present utility model, it may further have the following features: Among them, the number of fixing clamps is several, and several fixing clamps are arranged in a circle around the axial circumference of the first connecting rod and / or several fixing clamps are arranged vertically on the first connecting rod.

[0012] In the portable testicular tissue sampling and freezing device provided by the present utility model, it may further have the following features: Among them, the freezing assembly includes: a cryopreservation cup, which is a cup-shaped structure with an upward opening and is used to hold a low-temperature liquid; a cryopreservation cover, which is detachably arranged at the cup mouth of the cryopreservation cup; a second connecting rod, which is columnar and is arranged vertically on the lower bottom surface of the cryopreservation cover; and a cryopreservation box, which is detachably arranged on the second connecting rod and is used to store the freezing tube, and when the cryopreservation cover is fixed at the cup mouth of the cryopreservation cup, the cryopreservation box arranged on the second connecting rod is submerged by the low-temperature liquid in the cryopreservation cup.

[0013] In the portable testicular tissue sampling and freezing device provided by the present utility model, it may further have the following feature: wherein, the number of freezing boxes is several, and the several freezing boxes are detachably connected to the second connecting rod directly and / or indirectly in an up-and-down arrangement.

[0014] In the portable testicular tissue sampling and freezing device provided by the present utility model, it may further have the following feature: wherein, the freezing box includes: a box cover, which is a thick cover plate, and a female threaded hole with an upward opening is recessed in the center of its upper surface; a box body, which is a box-shaped structure with an upward opening, and its top is detachably connected to the bottom of the box cover; a fixing groove, which is arranged inside the box body and has an upward opening, and the fixing groove can be matched and embedded with a freezing tube; and a third connecting rod, which protrudes downward in the vertical direction from the center of the bottom of the box body, and both the bottom circumferences of the second connecting rod and the third connecting rod have external threads of the same specification and are all matched with the female threaded hole.

[0015] Functions and effects of the utility model

[0016] According to a portable testicular tissue sampling and freezing device involved in the present utility model, because it includes: a sampling component for sampling the testicular tissue to be processed; a processing component for washing and fixing the testicular tissue taken by the sampling component; a cooling component for cooling the testicular tissue processed by the processing component; and a freezing component for freezing the testicular tissue cooled by the cooling component, wherein the sampling component, the processing component, the cooling component, and the freezing component are all stored in a portable transport box.

[0017] Therefore, the portable testicular tissue sampling and freezing device of the present utility model can quickly obtain the testicular tissue of the treatment object and can also be quickly frozen, and at the same time is convenient for transportation and carrying. The portable testicular tissue sampling and freezing device of the present utility model can solve the problem of emergency fertility preservation for soldiers, making the equipment portable, being able to quickly collect the testicular tissue samples of the rescue object in a timely manner, and quickly freezing the testicular tissue for fertility preservation. Description of the drawings

[0018] Figure 1 is a perspective view of the portable testicular tissue sampling and freezing device in the embodiment of the present utility model;

[0019] Figure 2 is a perspective view of the storage cabinet when the transport main body is opened in the embodiment of the present utility model, wherein the first storage cabinet and the third storage cabinet are opened, and the second storage cabinet and the fourth storage cabinet are closed;

[0020] Figure 3 is a perspective view of the storage cabinet when the transport main body is opened in the embodiment of the present utility model, wherein the first storage cabinet and the third storage cabinet are closed, and the second storage cabinet and the fourth storage cabinet are opened;

[0021] Figure 4 It is a three-dimensional view of the physiological saline storage bottle in the embodiment of the present utility model;

[0022] Figure 5 It is a three-dimensional view of the porous culture plate in the embodiment of the present utility model;

[0023] Figure 6 It is a three-dimensional view of the porous tube rack in the embodiment of the present utility model;

[0024] Figure 7 It is a three-dimensional view of the cryotube in the embodiment of the present utility model;

[0025] Figure 8 It is a three-dimensional view of the cooling cup in the embodiment of the present utility model;

[0026] Figure 9 It is Figure 8 the corresponding cross-sectional view;

[0027] Figure 10 It is Figure 9 the enlarged view of area A in

[0028] Figure 11 It is a three-dimensional view of the cooling cover, the first connecting rod and the fixing pliers in the embodiment of the present utility model;

[0029] Figure 12 It is Figure 11 the corresponding three-dimensional view when the fixing pliers fix the cryotube;

[0030] Figure 13 It is a three-dimensional view of the cryopreservation cup in the embodiment of the present utility model;

[0031] Figure 14 It is a three-dimensional view of the cryopreservation cover, the second connecting rod and the cryopreservation box in the embodiment of the present utility model;

[0032] Figure 15 It is Figure 14 the corresponding front view;

[0033] Figure 16 It is the structural explosion view of the cryopreservation box in the embodiment of the present utility model. Detailed implementation manners

[0034] In order to make the technical means, creative features, achieved purposes and effects realized by the present utility model easy to understand, the following embodiments will specifically describe a portable testicular tissue sampling and freezing device of the present utility model in conjunction with the accompanying drawings.

[0035] <Embodiment>

[0036] Figure 1 It is a three-dimensional view of the portable testicular tissue sampling and freezing device in the embodiment of the present utility model;Figure 2 Fig. Figure 2 is a perspective view of the storage cabinet when the transfer body is opened in the embodiment of the present utility model. Among them, the first storage cabinet and the third storage cabinet are opened, and the second storage cabinet and the fourth storage cabinet are closed; Figure 3 Fig. Figure 3 is a perspective view of the storage cabinet when the transfer body is opened in the embodiment of the present utility model. Among them, the first storage cabinet and the third storage cabinet are closed, and the second storage cabinet and the fourth storage cabinet are opened.

[0037] As Figures 1 - 3 shown in Fig. Figures 1 - 3 , this embodiment provides a portable testicular tissue sampling and freezing device 100, which includes a transfer box 10, a sampling assembly 20, a processing assembly 30, a temperature reduction assembly 40, and a freezing assembly 50.

[0038] The transfer box 10 includes a transfer body 11, a handle 12, pulleys 13, and a storage cabinet 14.

[0039] The transfer body 11 is a box-shaped structure with an openable cover on one side and is a carbon fiber structure with a metal coating. Specifically, in this embodiment, the height of the transfer body 11 is 85 cm, the width is 45 cm, and the depth is 35 cm.

[0040] The handle 12 is stretchably arranged on the transfer body 11.

[0041] The number of pulleys 13 is 4, and they are arranged at the four corners of the bottom of the transfer body 11.

[0042] The storage cabinet 14 includes a first storage cabinet 141, a second storage cabinet 142, a third storage cabinet 143, and a fourth storage cabinet 144. All four are openable cabinets and are arranged inside the transfer body 11.

[0043] The first storage cabinet 141 has several storage compartments 1 inside, and the number is at least 5. Specifically, the number of storage compartments 1 in this embodiment is 6, and the 6 storage compartments 1 are evenly distributed.

[0044] The second storage cabinet 142 has several soft embedding grooves 2 with different shapes inside.

[0045] The inner wall of the third storage cabinet 143 is provided with a 1-cm-thick foam plastic as a heat insulation layer. The third storage cabinet 143 has 2 upward-opening circular grooves inside, denoted as the first matching grooves 3.

[0046] The inner wall of the fourth storage cabinet 144 is provided with a 1-cm-thick foam plastic as a heat insulation layer. The fourth storage cabinet 144 has 2 upward-opening circular grooves inside, denoted as the second matching grooves 4.

[0047] The sampling assembly 20 includes a sterile ophthalmic scissors and forceps, a puncture needle package, povidone iodine, sterile gauze, and a syringe. The above five items are separately placed in 5 different storage compartments 1 for the surgeon to take out and operate on when using to sample the patient's testicular tissue.

[0048] Figure 4 is the three-dimensional view of the physiological saline storage bottle in the embodiment of the present utility model; Figure 5 is the three-dimensional view of the multi-well culture plate in the embodiment of the present utility model; Figure 6 is the three-dimensional view of the multi-well tube rack in the embodiment of the present utility model; Figure 7 is the three-dimensional view of the cryotube in the embodiment of the present utility model.

[0049] As Figures 1 - 7 shown, the processing assembly 30 includes a physiological saline storage bottle 31, a multi-well culture plate 32, a multi-well tube rack 33, and a cryotube 34 containing cell cryoprotectant.

[0050] The physiological saline storage bottle 31 is embedded in one of the soft embedding grooves 2 that match its shape, and it stores physiological saline containing 1% antibiotic inside.

[0051] The multi-well culture plate 32 is embedded in one of the soft embedding grooves 2 that match its shape. Specifically, in this embodiment, the multi-well culture plate 32 is a six-well culture plate, and it has a total of 6 upward-opening circular groove-shaped culture dishes a for culturing testicular tissue. The multi-well culture plate 32 and the physiological saline storage bottle 31 cooperate with each other to wash the testicular tissue of the patient taken by the sampling assembly 20.

[0052] The multi-well tube rack 33 is a thick plate structure. Specifically, in this embodiment, the multi-well tube rack 33 is a 48-well cryotube rack, and it is provided with 48 upward-opening cylindrical fixing grooves b.

[0053] The number of cryotubes 34 is several, and they are correspondingly and fittingly embedded and stored in the fixing grooves b. The several cryotubes 34 and the multi-well tube rack 33 cooperate with each other to provide a fixing environment for the washed testicular tissue of the patient. Specifically, in this embodiment, the cryotubes 34 are 2 mL-sized cryotubes.

[0054] The cooling assembly 40 includes a cooling cup 41, an anti-falling element 42, a cooling cover 43, a first connecting rod 44, and a fixing clamp 45.

[0055] Figure 8 is the three-dimensional view of the cooling cup in the embodiment of the present utility model.

[0056] As Figures 1 - 8As shown, the cooling cup 41 is a bottomed cylindrical cup-shaped structure with an upward opening, used for holding low-temperature liquid (in this embodiment, the low-temperature liquid is liquid nitrogen). Specifically, in this embodiment, the height of the cup body of the cooling cup 41 is 28 cm, and the inner diameter of the cup body is 8 cm; the number of cooling cups 41 is 2, which can be respectively and fittingly embedded in 2 first matching grooves 3; an internal thread is provided around the inner wall of the top opening of the cooling cup 41, denoted as the first internal thread 5-1.

[0057] Figure 9 is Figure 8 the corresponding cross-sectional view; Figure 10 is Figure 9 the enlarged view of area A in

[0058] As Figures 1 - 9 shown, the anti-falling element 42 includes a circular plate 421 and a through hole 422.

[0059] The circular plate 421 is a circular plate-shaped structure matching the inner diameter of the cooling cup 41, and is arranged in the middle section of the cup body of the cooling cup 41. Specifically, in this embodiment, the circular plate 421 is 20 cm away from the bottom of the cup body of the cooling cup 41 and 8 cm away from the top of the cup body of the cooling cup 41.

[0060] In this embodiment, the liquid nitrogen poured into the cooling cup 41 does not exceed the plane where the circular plate 421 is located.

[0061] The number of through holes 422 is several, passing through the circular plate 421 and distributed in an array. Specifically, in this embodiment, the inner diameter of the through hole 422 is 0.5 cm.

[0062] Figure 11 is the three-dimensional view of the cooling cover, the first connecting rod and the fixing pliers in the embodiment of the present utility model; Figure 12 is Figure 11 the corresponding three-dimensional view when the fixing pliers fix the freezing tube.

[0063] As Figures 1 - 12 shown, the cooling cover 43 is a 2-cm thick circular plate structure, with a coaxial cylindrical protrusion convexly provided at the bottom, the diameter of the protrusion is smaller than the diameter of the cooling cover 43 and an external thread matching the first internal thread 5-1 is provided around it, denoted as the first external thread 5-2. Through the matching screw connection of the first external thread 5-2 and the first internal thread 5-1, the cooling cover 43 is detachably fixed on the top opening of the cooling cup 41.

[0064] The first connecting rod 44 is in the shape of a cylindrical rod and is vertically fixed below the cylindrical protrusion on the lower bottom surface of the cooling cover 43. When the cooling cover 43 is screwed and fixed to the cup mouth of the cooling cup 41, the first connecting rod 44 is located inside the cup body of the cooling cup 41 and completely above the circular plate 421. Specifically, in this embodiment, when the cooling cover 43 is screwed and fixed to the cup mouth of the cooling cup 41, the bottom of the first connecting rod 44 abuts against the upper surface of the circular plate 421.

[0065] The fixing clamp 45 is a clamp-shaped structure with shrinkable elasticity and can clamp the freezing tube 34 in a matching manner. In this embodiment, 4 fixing clamps 45 are evenly arranged in a circle around the axial circumference of the first connecting rod 44 to form a group, which can be used to clamp 4 freezing tubes 34. Two groups of a total of 8 fixing clamps 45 are arranged one above the other, and a total of 8 freezing tubes 34 can be clamped. In this embodiment, when the cooling cover 43 is screwed and fixed to the cup mouth of the cooling cup 41, the fixing clamp 45 and the freezing tube 34 clamped thereon are both located inside the cup body of the cooling cup 41 and completely above the circular plate 421.

[0066] The freezing assembly 50 includes a freezing cup 51, a freezing cover 52, a second connecting rod 53, and a freezing box 54.

[0067] Figure 13 It is a perspective view of the freezing cup in the embodiment of the present utility model.

[0068] As Figures 1 - 13 shown, the freezing cup 51 is a bottomed cylindrical cup-shaped structure with an upward opening and a height of 28 cm, and its inner diameter is 8 cm, which is used to hold a low-temperature liquid (in this embodiment, the low-temperature liquid is liquid nitrogen); the number of freezing cups 51 is 2, and they can be respectively embedded in 2 second matching grooves 4 in a matching manner; an internal thread is arranged in a circle around the inner wall of the top opening of the freezing cup 51, denoted as the second internal thread 6-1.

[0069] Figure 14 It is a perspective view of the freezing cover, the second connecting rod, and the freezing box in the embodiment of the present utility model; Figure 15 It is Figure 14 the corresponding front view.

[0070] As Figures 1 - 15 shown, the freezing cover 52 is a circular plate structure with a thickness of 2 cm, and a coaxial cylindrical protrusion protrudes from its bottom. The diameter of the protrusion is smaller than the diameter of the freezing cover 52, and an external thread that matches the second internal thread 6-1 is arranged in a circle around it, denoted as the second external thread 6-2. Through the matching screw connection of the second internal thread 6-1 and the second external thread 6-2, the freezing cover 52 is detachably fixed to the top opening of the freezing cup 51.

[0071] The second connecting rod 53 is a cylindrical rod, vertically fixed below the cylindrical protrusion on the lower bottom surface of the freezing cover 52, and when the freezing cover 52 is screwed onto the cup mouth of the freezing cup 51, the second connecting rod 53 is located inside the body of the freezing cup 51; an external thread is provided on the circumferential side of the bottom of the cylindrical second connecting rod 53, denoted as the third external thread (not shown in the figure).

[0072] Figure 16 It is an exploded view of the structure of the freezing box in the embodiment of the present utility model.

[0073] As Figures 1 - 16 shown, the freezing box 54 includes a box cover 541, an internal thread hole 542, a box body 543, a fixing groove 544, and a third connecting rod 545.

[0074] The box cover 541 is a circular thick cover plate, with a coaxial cylindrical protrusion protruding from its bottom. The diameter of the protrusion is smaller than the diameter of the box cover 541, and an external thread is provided around it, denoted as the fourth external thread 8-1.

[0075] The internal thread hole 542 is provided at the center of the upper surface of the box cover 541 and opens upward. The internal thread hole 542 matches the third external thread 7, so that the bottom of the second connecting rod 53 can be screwed and fixed in the internal thread hole 542.

[0076] The box body 543 is a bottomed cylindrical box-like structure with an upward opening. An internal thread is provided around the inner wall of the top opening, denoted as the fourth internal thread 8-2. Among them, the fourth internal thread 8-2 matches the fourth external thread 8-1, so that the box cover 541 can be screwed and fixed to the top opening of the box body 543.

[0077] In a variant of this embodiment, the box cover 541 is a rectangular thick cover plate, and the box body 543 is a bottomed rectangular structure with an upward opening. The two match each other to form a metal freezing box of 4×4×3.5 cm.

[0078] The number of fixing grooves 544 is 4, recessed in the box body 543 and evenly distributed with upward openings. The fixing grooves 544 match the freezing tubes 34 so that they can be embedded and stored.

[0079] The third connecting rod 545 is a cylindrical rod. One end is vertically fixed at the center of the bottom of the box body 543, and the other end has an external thread, denoted as the fifth external thread 7. Among them, the fifth external thread 7 has the same specification as the third external thread, so that the bottom of the third connecting rod 545 can be screwed and fixed to match the internal thread hole 542 through the fifth external thread 7.

[0080] In this embodiment, the number of cryopreservation boxes 54 is 4. One of the cryopreservation boxes 54 is screwed and fixed to the second connecting rod 53 through the matching of the internal threaded hole 542 and the third external thread. The remaining three cryopreservation boxes 54 are respectively screwed and fixed end to end through their respective internal threaded holes 542 and the fifth external thread 7 on the third connecting rod 545 of another one, so that the four cryopreservation boxes 54 are connected end to end in a vertical row, and the overall height formed by the four cryopreservation boxes 54 is 16 cm. When the cryopreservation cover 52 is screwed and fixed to the cup mouth of the cryopreservation cup 51, the four cryopreservation boxes 54 connected and fixed below the cryopreservation cover 52 are completely submerged by the liquid nitrogen in the cryopreservation cup 51.

[0081] The use process of the portable testicular tissue sampling and freezing device 100:

[0082] S10, open the transport body 11, and use the iodine tincture and sterile gauze stored in different storage compartments 1 in the first storage cabinet 141 to disinfect the patient's perineum with iodine tincture gauze;

[0083] S20, using the puncture needle packs and syringes stored in different storage compartments 1 in the first storage cabinet 141 to quickly puncture and collect the patient's testicular tissue;

[0084] S30, using sterile ophthalmic scissors and forceps stored in different storage compartments 1 in the first storage cabinet 141 to transfer the collected testicular tissue to the multi-well culture plate 32 in the second storage cabinet 142, and washing it with physiological saline containing 1% antibiotics in the physiological saline storage bottle 31 in the second storage cabinet 142;

[0085] S40, using forceps to transfer the washed testicular tissue into a freezing tube 34 set on a porous tube rack 33 and wait for 10 to 20 minutes;

[0086] S50, a plurality of cryotubes 34 containing testicular tissues are clamped and fixed to the fixing clamp 45 of the cooling assembly 40 in the third storage cabinet 143, and then the cooling cover 43 is screwed and fixed to the cup mouth of the cooling cup 41, and the liquid nitrogen in the cooling cup 41 is used to fumigate and cool the plurality of cryotubes 34 through the anti-falling element 42 for at least 30 minutes;

[0087] S60, the cooled cryotube 34 is placed into the freezing box 54 of the freezing assembly 50 stored in the fourth storage cabinet 144, and the freezing cover 52 is screwed and fixed to the mouth of the freezing cup 51, so that the liquid nitrogen in the freezing cup 51 completely submerges the four freezing boxes 54 in a row. Finally, the freezing cup 51 is placed back into the second matching slot 4.

[0088] S70. Organize each surgical instrument, close the first storage cabinet 141, the second storage cabinet 142, the third storage cabinet 143, the fourth storage cabinet 144, and the transfer main body 11, and then the entire portable testicular tissue sampling and freezing device 100 can be moved.

[0089] Functions and effects of the embodiment

[0090] A portable testicular tissue sampling and freezing device provided according to this embodiment includes: a sampling component for sampling the testicular tissue to be processed; a processing component for washing and fixing the testicular tissue taken by the sampling component; a cooling component for cooling the testicular tissue processed by the processing component; and a freezing component for freezing the testicular tissue cooled by the cooling component. Among them, the sampling component, the processing component, the cooling component, and the freezing component are all stored in a portable transfer box.

[0091] Therefore, the portable testicular tissue sampling and freezing device of this embodiment can quickly obtain the testicular tissue of the treatment object, can also be quickly frozen, and is convenient for transportation and carrying at the same time. The portable testicular tissue sampling and freezing device of the present invention can solve the problem of emergency fertility preservation for soldiers, making the device portable, capable of quickly collecting the testicular tissue sample of the rescue object in a timely manner, and quickly freezing the testicular tissue for fertility preservation.

[0092] Furthermore, the transfer box includes: a transfer main body, which is a box-shaped structure with an openable cover on one side; a handle, stretchably arranged on the transfer main body; a plurality of pulleys, arranged at the bottom of the transfer main body; and 4 storage cabinets, arranged inside the transfer main body, respectively used for correspondingly placing the sampling component, the processing component, the cooling component, and the freezing component. Such a setting makes the entire portable testicular tissue sampling and freezing device easy to carry at any time.

[0093] Furthermore, the sampling component includes a sterile ophthalmic scissors and forceps, a puncture needle package, iodine, sterile gauze, and a syringe, which are separated and placed. The processing component includes: a multi-well culture plate and a physiological saline storage bottle, for the surgeon to use to wash the testicular tissue taken by the sampling component; and a freezing tube containing cell cryoprotectant and a multi-well tube rack matching the freezing tube, used as the storage environment for the washed testicular tissue. Such a setting enables the surgeon to quickly perform perineal cleaning, disinfection, and testicular sampling operations on the patient using the portable testicular tissue sampling and freezing device.

[0094] Further, the temperature reduction assembly includes: a temperature reduction cup, which is a cup-shaped structure with an upward opening and is used for containing a low-temperature liquid; a temperature reduction cover, which is detachably arranged at the cup mouth of the temperature reduction cup; a first connecting rod, which is columnar and is arranged vertically on the lower bottom surface of the temperature reduction cover, and when the temperature reduction cover is fixed at the cup mouth of the temperature reduction cup, the first connecting rod is located inside the cup body of the temperature reduction cup; and a fixing clamp, which is arranged on the first connecting rod, and the fixing clamp is a clamp-shaped structure with shrinkage elasticity and can be matched to clamp the freezing tube. With such a setting, the sampled testicular tissue can be conveniently cooled before cryopreservation.

[0095] Further, the temperature reduction assembly further includes an anti-falling element, and the anti-falling element is a filter screen or a perforated plate that matches the inner diameter of the temperature reduction cup. The anti-falling element is arranged in the middle section inside the cup body of the temperature reduction cup. The liquid level of the low-temperature liquid in the temperature reduction cup is lower than the position where the anti-falling element is located, and the positions of the first connecting rod, the fixing clamp, and the freezing tube fixed on the fixing clamp are all higher than the position where the anti-falling element is located. With such a setting, the freezing tube will not fall into the liquid nitrogen during the temperature reduction process, making it more difficult to take out.

[0096] Further, the number of fixing clamps is several, and several fixing clamps are arranged around the axial circumference of the first connecting rod, and several fixing clamps are arranged vertically on the first connecting rod. With such a setting, several freezing tubes can be evenly arranged and fixed on the first connecting rod through several fixing clamps as much as possible.

[0097] Further, the freezing assembly includes: a freezing cup, which is a cup-shaped structure with an upward opening and is used for containing a low-temperature liquid; a freezing cover, which is detachably arranged at the cup mouth of the freezing cup; a second connecting rod, which is columnar and is arranged vertically on the lower bottom surface of the freezing cover; and a freezing box, which is detachably arranged on the second connecting rod and is used for storing the freezing tube, and when the freezing cover is fixed at the cup mouth of the freezing cup, the freezing box arranged on the second connecting rod is submerged by the low-temperature liquid in the freezing cup. With such a setting, the sampled testicular tissue stored in the freezing tube can be quickly and conveniently cryopreserved.

[0098] Further, the number of freezing boxes is several, and several freezing boxes are directly and / or indirectly detachably connected to the second connecting rod in a vertical arrangement. With such a setting, several freezing boxes are arranged vertically in sequence, which is convenient to be smoothly placed in the freezing cup through the freezing cover.

[0099] Further, the cryopreservation box includes: a box cover, which is a thick cover plate, and a threaded hole with an upward opening is recessed in the center of its upper surface; a box body, which is a box-shaped structure with an upward opening, and its top is detachably connected to the bottom of the box cover; a fixing groove, which is arranged inside the box body and has an upward opening, and the fixing groove can be matched and embedded with a cryotube; and a third connecting rod, which protrudes downward in the vertical direction from the center of the bottom of the box body. The bottom circumferences of the second connecting rod and the third connecting rod both have the same specifications and external threads that are all matched with the threaded hole. With such a setting, the same cryopreservation box can evenly fix multiple cryotubes, so that the same freezing assembly can store as many cryotubes as possible.

[0100] Those skilled in the art should understand that the present utility model is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principle of the present utility model. Without departing from the spirit and scope of the present utility model, the present utility model will have various changes and improvements, and these changes and improvements all fall within the scope of the present utility model claimed. The scope of protection claimed by the present utility model is defined by the appended claims and their equivalents.

Claims

1. A portable testicular tissue sampling and freezing device, characterized in that: include: A sampling assembly, used for sampling the testicular tissue to be processed; A processing component, used for washing and fixing the testicular tissue collected by the sampling component; A cooling component, used for cooling the testicular tissue after being processed by the processing component; as well as A freezing component is used to freeze the testicular tissue after the cooling component has cooled down. Wherein, the sampling component, the processing component, the cooling component and the freezing component are all stored in a portable transfer box.

2. The portable testicular tissue sampling and freezing device according to claim 1, Features: Wherein, the transfer box comprises: The transfer body is a box-shaped structure with an openable cover on one side; A handle, stretchably disposed on the transport body; Pulleys, a number of which are arranged at the bottom of the transport body; Storage cabinets, 4 in number, are arranged inside the transport body and are used to place the sampling component, the processing component, the cooling component and the freezing component respectively.

3. The portable testicular tissue sampling and freezing device according to claim 1, characterized in that: in, The sampling components include sterile ophthalmic scissors and tweezers, a puncture needle pack, iodine tincture, sterile gauze and a syringe, which are placed separately.

4. The portable testicular tissue sampling and freezing device according to claim 1, Features: Wherein, the processing component includes: A multi-well culture plate and a physiological saline storage bottle, for use by a surgeon to wash the testicular tissue collected by the sampling assembly; and The freezing tube containing the cell freezing solution and the multi-hole tube rack matched with the freezing tube are used as the storage environment of the washed testicular tissue.

5. The portable testicular tissue sampling and freezing device according to claim 4, Features: Wherein, the cooling component comprises: The cooling cup is a cup-shaped structure with an upward opening, used to hold cryogenic liquid; A cooling cover is detachably arranged on the cup mouth of the cooling cup; a first connecting rod, which is columnar and vertically disposed on the lower bottom surface of the cooling cover, and when the cooling cover is fixed to the cup mouth of the cooling cup, the first connecting rod is located inside the cup body of the cooling cup; and The fixing clamp is arranged on the first connecting rod, and the fixing clamp is a clamp-shaped structure with contraction elasticity, which can clamp the freezing pipe in a matching manner.

6. The portable testicular tissue sampling and freezing device according to claim 5, characterized in that: in, The cooling assembly also includes a fall prevention element, The anti-falling element is a filter screen or a perforated plate that matches the inner diameter of the cooling cup. The anti-falling element is arranged in the middle section of the inner body of the cooling cup. The liquid level of the low-temperature liquid in the cooling cup is lower than the position of the anti-falling element, and the positions of the first connecting rod, the fixing clamp and the freezing pipe fixed on the fixing clamp are all higher than the position of the anti-falling element.

7. The portable testicular tissue sampling and freezing device according to claim 5, characterized in that: in, The number of the fixed clamps is several, A plurality of the fixing clamps are arranged around the axial circumference of the first connecting rod, and / or a plurality of the fixing clamps are arranged up and down on the first connecting rod.

8. The portable testicular tissue sampling and freezing device according to claim 4, Features: Wherein, the freezing component comprises: The cryo-storage cup is a cup-shaped structure with an upward opening, used to hold cryogenic liquids; A freezing cover is detachably arranged on the mouth of the freezing cup; A second connecting rod is columnar and vertically disposed on the lower surface of the freezing cover; and A freezing box is detachably mounted on the second connecting rod and is used to store the freezing tube. When the freezing cover is fixed to the mouth of the freezing cup, the freezing box mounted on the second connecting rod is submerged in the low-temperature liquid in the freezing cup.

9. The portable testicular tissue sampling and freezing device according to claim 8, characterized in that: in, The number of the freezing boxes is several, A plurality of the freezing boxes are arranged vertically and directly and / or indirectly detachably connected to the second connecting rod.

10. The portable testicular tissue sampling and freezing device according to claim 9, Features: Wherein, the freezing box comprises: The box cover is a thick cover plate, and a recessed inner threaded hole with an opening facing upward is arranged at the center of the upper surface; The box body is a box-shaped structure with an upward opening, the top of which is detachably connected to the bottom of the box cover; A fixing groove is arranged inside the box body and has an opening facing upward, and the fixing groove can be matched and embedded with the freezing tube; and The third connecting rod is vertically protruding downward at the center of the bottom of the box body. The bottom circumferences of the second connecting rod and the third connecting rod are both provided with external threads of the same specification and matching with the internal threaded hole.

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