Colloidal gold test paper card for rapidly detecting canine coronavirus antibody

Abstract

The utility model provides a colloidal gold test paper card for rapidly detecting canine coronavirus antibodies, which relates to the technical field of colloidal gold test paper cards, and comprises a colloidal gold test paper card body, the top of the colloidal gold test paper card body is provided with a sample adding structure, and the sample adding structure can prevent overflow in the sample adding process. A storage structure is further arranged in the colloidal gold test paper card body and close to the back side, the storage structure enables the test paper card to be dry during long-term storage, and the sample adding process is easier through the funnel-shaped design of the large caliber of the sample adding structure; the possibility of inconvenient operation and sample overflow caused by too small sample adding port is reduced, the sample adding is more convenient, the waste of the sample is reduced, the detection error caused by non-uniform or insufficient sample adding is reduced, the filter membrane at the bottom of the sample adding structure can effectively prevent large-particle impurities from entering a chromatography channel, the purity of the detection process is guaranteed, and the detection efficiency is improved. The accuracy of a detection result is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of colloidal gold test strips, in particular to a colloidal gold test strip for rapidly detecting canine coronavirus antibodies. Background Art

[0002] In the field of diagnosing canine diseases, it is crucial to rapidly and accurately detect canine coronavirus antibodies. Traditional detection methods often require complex instrument equipment and professional operation skills, which not only increase the detection cost and time but also limit their application in grass-roots veterinary clinics and self-detection by pet owners. With the continuous development of biotechnology, colloidal gold test strips have emerged as a convenient and rapid detection tool.

[0003] The sample addition port of the existing colloidal gold test strip is too small, resulting in difficult sample addition, easy sample overflow, and affecting the accuracy of the detection result. At the same time, the storage conditions of the colloidal gold test strip have a crucial impact on the accuracy and reliability of the detection result. The detection reagents in the test strip are usually sensitive to environmental factors such as humidity, temperature, and light. Improper long-term storage easily leads to reagent failure, thus affecting the detection accuracy. Moreover, the color development intensity of the test line and the control line is sometimes not ideal. Especially when the antibody concentration in the sample is low, the color development may be weak, making it difficult to accurately identify and judge, and prone to false judgment or missed judgment. Therefore, we propose a colloidal gold test strip for rapidly detecting canine coronavirus antibodies. Summary of the Utility Model

[0004] The purpose of the utility model is to solve the deficiencies existing in the prior art. The sample addition port of the existing colloidal gold test strip is too small, resulting in difficult sample addition, easy sample overflow, and affecting the accuracy of the detection result. At the same time, the storage conditions of the colloidal gold test strip have a crucial impact on the accuracy and reliability of the detection result. The detection reagents in the test strip are usually sensitive to environmental factors such as humidity, temperature, and light. Improper long-term storage easily leads to reagent failure, thus affecting the detection accuracy. Moreover, the color development intensity of the test line and the control line is sometimes not ideal. Especially when the antibody concentration in the sample is low, the color development may be weak, making it difficult to accurately identify and judge, and prone to false judgment or missed judgment.

[0005] To achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A colloidal gold test strip for rapid detection of canine coronavirus antibodies, comprising a colloidal gold test strip body. A sample adding structure is provided at the top of the colloidal gold test strip body, and the sample adding structure can prevent overflow during the sample adding process. A storage structure is further provided inside the colloidal gold test strip body and near the dorsal side, and the storage structure can keep the test strip dry during long-term storage, avoiding dampness. An installation groove is also opened at the bottom inside the colloidal gold test strip body, and a reflective pad is provided inside the installation groove.

[0007] Preferably, a sample absorption pad is provided inside the colloidal gold test strip body and near the front side. A gold-labeled antigen pad is provided at the bottom of the sample absorption pad. A gold-labeled mouse IgG pad is provided at the bottom of the gold-labeled antigen pad. A nitrocellulose NC membrane is provided on the right side of the gold-labeled mouse IgG pad. An absorbent pad is provided on the right side of the nitrocellulose NC membrane.

[0008] Preferably, an observation member is provided at the center of the top of the colloidal gold test strip body. The reflective pad and the observation member are used in cooperation with each other, and the material of the reflective pad is aluminized polyester film.

[0009] Preferably, the sample adding structure includes an expansion port, a connection port is provided inside the expansion port, and a filter membrane is provided inside the connection port.

[0010] Preferably, the expansion port is sleeved outside the connection port, and the expansion port and the connection port are threadedly connected. The filter membrane and the connection port are fixedly connected.

[0011] Preferably, the storage structure includes a partition board, a breathable and waterproof membrane is provided inside the partition board, a placement space is provided between the partition board and the colloidal gold test strip body, and a plurality of desiccant blocks are provided inside the placement space.

[0012] Preferably, the breathable and waterproof membrane is fixedly installed inside the partition board, and the material of the breathable and waterproof membrane is polytetrafluoroethylene (PTFE) membrane.

[0013] Compared with the prior art, the beneficial effects of the present utility model are:

[0014] 1. The large-diameter funnel-shaped design of the sample adding structure makes the sample adding process easier, reduces the possibility of inconvenient operation and sample overflow caused by too small a sample adding port, makes sample adding more convenient, reduces sample waste, and reduces the detection error caused by uneven or insufficient sample adding. The filter membrane at the bottom of the sample adding structure can effectively block large-particle impurities from entering the chromatography channel, ensure the purity of the detection process, and improve the accuracy of the detection result.

[0015] 2. The storage structure extends the shelf life of the test strip card, ensures the stability and effectiveness of the test reagent, and reduces the risk of inaccurate test results caused by moisture.

[0016] 3. The reflective pad arranged below the observation piece further optimizes the color development contrast. This enables the test line and control line of the observation piece to maintain a distinct color development difference under different lighting conditions, including strong light, weak light, or complex ambient light, without being interfered by changes in external light. Whether in the ideal lighting environment of a laboratory or various actual light conditions during on-site testing, it can ensure that the test results are clearly distinguishable. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 Schematic diagram of the main body of a colloidal gold test strip card for rapid detection of canine coronavirus antibodies provided by the present utility model;

[0018] Figure 2 Internal structure schematic diagram of a colloidal gold test strip card for rapid detection of canine coronavirus antibodies provided by the present utility model;

[0019] Figure 3 Expanded schematic diagram of the sample adding structure of a colloidal gold test strip card for rapid detection of canine coronavirus antibodies provided by the present utility model;

[0020] Figure 4 Schematic diagram of the installation positions of the installation groove and the reflective pad of a colloidal gold test strip card for rapid detection of canine coronavirus antibodies provided by the present utility model

[0021] Figure 5 Top view structure schematic diagram of a colloidal gold test strip card for rapid detection of canine coronavirus antibodies provided by the present utility model.

[0022] Legend: 1. Colloidal gold test strip card body; 2. Sample adding structure; 21. Expansion port; 22. Connection port; 23. Filter membrane; 3. Storage structure; 31. Partition board; 32. Breathable and waterproof membrane; 33. Placement room; 34. Desiccant block; 4. Sample absorption pad; 5. Gold-labeled antigen pad; 6. Gold-labeled mouse IgG pad; 7. Nitrocellulose NC membrane; 8. Observation piece; 9. Installation groove; 10. Reflective pad; 11. Absorbent pad. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0024] To facilitate the understanding of the present utility model, the following will provide a more comprehensive description of the present utility model with reference to the relevant content. Several embodiments of the present utility model are given. However, the present utility model can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present utility model more thorough and comprehensive.

[0025] It should be noted that when an element is referred to as being "fixedly provided on" another element, it can be directly on the other element or there may also be an intermediate element. When an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are only for the purpose of illustration.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present utility model belongs. The terms used in the description of the present utility model herein are only for the purpose of describing specific embodiments and are not intended to limit the present utility model. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0027] Embodiment 1

[0028] As Figures 1-5As shown in the figure, the present utility model provides a technical solution: a colloidal gold test strip card for rapid detection of canine coronavirus antibodies, including a colloidal gold test strip card body 1. A sample adding structure 2 is provided at the top of the colloidal gold test strip card body 1, and the sample adding structure 2 can prevent overflow during the sample adding process. A storage structure 3 is further provided inside the colloidal gold test strip card body 1 and near the dorsal side. The storage structure 3 can keep the test strip card dry during long-term storage, avoiding dampness. An installation groove 9 is also opened at the bottom inside the colloidal gold test strip card body 1, and a reflective pad 10 is provided inside the installation groove 9. The larger-diameter funnel-shaped design of the sample adding structure 2 makes the sample adding process easier, reduces the possibility of inconvenient operation and sample overflow caused by too small a sample adding port, makes sample adding more convenient, reduces sample waste, and reduces the detection error caused by uneven or insufficient sample adding. The filter membrane 23 at the bottom of the sample adding structure 2 can effectively block large particle impurities from entering the chromatographic channel, ensuring the purity of the detection process and improving the accuracy of the detection result. The storage structure 3 extends the shelf life of the test strip card, ensures the stability and effectiveness of the detection reagent, and reduces the risk of inaccurate detection results caused by dampness. The reflective pad 10 provided below the observation piece 8 further optimizes the color development contrast, so that the test line and control line of the observation piece 8 can maintain a distinct color development difference under different light conditions, including strong light, weak light or complex ambient light, without being interfered by changes in external light. Whether in the ideal lighting environment of the laboratory or under various actual light conditions of on-site detection, the detection result can be ensured to be clearly distinguishable.

[0029] Embodiment 2

[0030] As Figures 1-5 shown in the figure, the present utility model provides a technical solution: a sample absorption pad 4 is provided inside the colloidal gold test strip card body 1 and near the front side. A gold-labeled antigen pad 5 is provided at the bottom of the sample absorption pad 4. A gold-labeled mouse IgG pad 6 is provided at the bottom of the gold-labeled antigen pad 5. A nitrocellulose NC membrane 7 is provided on the right side of the gold-labeled mouse IgG pad 6. An absorbent pad 11 is provided on the right side of the nitrocellulose NC membrane 7.

[0031] An observation piece 8 is provided at the top and at the center of the colloidal gold test strip card body 1. The reflective pad 10 and the observation piece 8 are used in cooperation with each other. The material of the reflective pad 10 is aluminized polyester film. The reflective pad 10 can reflect light from the outside, further improving the color development contrast and making the detection result easier to observe and judge. In this way, even if the content of canine coronavirus antibodies in the sample is low, it can be more clearly detected through the enhanced color development effect. When long-term storage is required, and the material of the reflective pad 10 is aluminized polyester film, aluminized polyester film is a layer of aluminum plated on the surface of polyester film, which has good reflective performance and relatively low cost.

[0032] The sample adding structure 2 includes an expansion port 21. Inside the expansion port 21, there is a connection port 22. Inside the connection port 22, there is a filter membrane 23. When it is necessary to add a sample, the sample is directly poured onto the expansion port 21. Since the opening of the expansion port 21 is relatively large, the sample will quickly flow through the expansion port 21 and the connection port 22 to the filter membrane 23. The filter membrane 23 can filter the sample. The filter membrane 23 can effectively block large particle impurities from entering the chromatographic channel, ensuring the purity of the detection process and improving the accuracy of the detection result.

[0033] The expansion port 21 is sleeved outside the connection port 22, and the expansion port 21 and the connection port 22 are threadedly connected. The filter membrane 23 and the connection port 22 are fixedly connected.

[0034] The storage structure 3 includes a partition board 31. Inside the partition board 31, there is a breathable and waterproof membrane 32. Between the partition board 31 and the colloidal gold test strip card body 1, there is a placement space 33. Inside the placement space 33, there are several desiccant blocks 34. When long-term storage is required, and the material of the reflective pad 10 is aluminized polyester film. The aluminized polyester film is coated with a layer of aluminum on the surface of the polyester film, which has good reflective performance and relatively low cost. At the same time, due to the setting of the storage structure 3, the desiccant blocks 34 absorb the internal moisture through the breathable and waterproof membrane 32 to maintain a dry environment for the test strip. The material of the breathable and waterproof membrane 32 is polytetrafluoroethylene (PTFE) membrane. The polytetrafluoroethylene (PTFE) membrane has good chemical stability, high and low temperature resistance, and breathability, and can effectively block the passage of moisture at the same time.

[0035] The breathable and waterproof membrane 32 is fixedly installed inside the partition board 31, and the material of the breathable and waterproof membrane 32 is polytetrafluoroethylene (PTFE) membrane.

[0036] The working process of the present utility model: When using a colloidal gold test strip card for rapid detection of canine coronavirus antibodies, first, when it is necessary to add a sample, the sample is directly poured into the expansion port 21. Since the opening of the expansion port 21 is relatively large, the sample will quickly flow through the expansion port 21 and the connection port 22 to the filter membrane 23. The filter membrane 23 can filter the sample. The filter membrane 23 can effectively block large particle impurities from entering the chromatography channel, ensuring the purity of the detection process and improving the accuracy of the detection result. Subsequently, the sample will reach the sample absorption pad 4 and reach the nitrocellulose NC membrane 7 through the sample absorption pad 4, the gold-labeled antigen pad 5, and the gold-labeled mouse IgG pad 6, and will be displayed on the nitrocellulose NC membrane 7. When the sample contains canine coronavirus antibodies and chromatographs on the test strip card, the antibody-gold complex will be captured at the test line. The reflective pad 10 can reflect the light from the outside, further enhancing the color contrast and making the detection result easier to observe and judge. In this way, even if the content of canine coronavirus antibodies in the sample is relatively low, it can be more clearly detected through the enhanced color display effect. When long-term storage is required, and the material of the reflective pad 10 is aluminized polyester film. The aluminized polyester film is coated with a layer of aluminum on the surface of the polyester film, which has good reflective performance and relatively low cost. At the same time, due to the setting of the storage structure 3, the desiccant block 34 absorbs the internal moisture through the breathable waterproof membrane 32 to maintain a dry environment for the test strip card. The material of the breathable waterproof membrane 32 is polytetrafluoroethylene (PTFE) membrane. The polytetrafluoroethylene (PTFE) membrane has good chemical stability, high and low temperature resistance, and breathability, and can effectively block the passage of moisture.

[0037] Although the embodiments of the present utility model have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present utility model. The scope of the present utility model is defined by the appended claims and their equivalents.

Claims

1. A colloidal gold test paper card for rapid detection of canine coronavirus antibodies, comprising a colloidal gold test paper card body (1), characterized in that: The colloidal gold test paper card body (1) is provided with a sample loading structure (2) on the top, and the sample loading structure (2) can prevent overflow during the sample loading process. The colloidal gold test paper card body (1) is also provided with a storage structure (3) near the back side, and the storage structure (3) can make the test paper card dry and avoid moisture during long-term storage. The colloidal gold test paper card body (1) is also provided with a mounting groove (9) at the bottom, and a reflective pad (10) is provided inside the mounting groove (9).

2. A colloidal gold test paper card for rapid detection of canine coronavirus antibodies according to claim 1, characterized in that: A sample absorption pad (4) is provided inside the colloidal gold test paper card body (1) and near the front, a gold-labeled antigen pad (5) is provided at the bottom of the sample absorption pad (4), a gold-labeled mouse IgG pad (6) is provided at the bottom of the gold-labeled antigen pad (5), a nitrocellulose NC membrane (7) is provided on the right side of the gold-labeled mouse IgG pad (6), and a water absorption pad (11) is provided on the right side of the nitrocellulose NC membrane (7).

3. A colloidal gold test paper card for rapid detection of canine coronavirus antibodies according to claim 2, characterized in that: An observation piece (8) is provided at the top and center of the colloidal gold test paper card body (1); the reflective pad (10) and the observation piece (8) are used in conjunction with each other; and the reflective pad (10) is made of aluminum-plated polyester film.

4. A colloidal gold test paper card for rapid detection of canine coronavirus antibodies according to claim 3, characterized in that: The sample loading structure (2) comprises an expansion port (21), a connection port (22) is provided inside the expansion port (21), and a filter membrane (23) is provided inside the connection port (22).

5. A colloidal gold test paper card for rapid detection of canine coronavirus antibodies according to claim 4, characterized in that: The expansion port (21) is sleeved on the outside of the connection port (22), and the expansion port (21) and the connection port (22) are threadedly connected, and the filter membrane (23) and the connection port (22) are fixedly connected.

6. A colloidal gold test paper card for rapid detection of canine coronavirus antibodies according to claim 1, characterized in that: The storage structure (3) comprises a partition plate (31), a breathable waterproof membrane (32) is provided inside the partition plate (31), a placement room (33) is provided between the partition plate (31) and the colloidal gold test paper card body (1), and a plurality of desiccant blocks (34) are provided inside the placement room (33).

7. A colloidal gold test paper card for rapid detection of canine coronavirus antibodies according to claim 6, characterized in that: The breathable waterproof membrane (32) is fixedly installed inside the partition plate (31), and the material of the breathable waterproof membrane (32) is a polytetrafluoroethylene (PTFE) membrane.

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