Separation equipment for bone marrow stem cell extraction

Abstract

The utility model relates to the technical field of cell separation, in particular to separation equipment for bone marrow stem cell extraction, which comprises a rotary drum, a stem cell separation bottle arranged on the rotary drum and a driving part for driving the rotary drum to rotate, a hand lifting part is arranged on the outer wall of the upper end of the rotary drum, and the rotary drum is detachably arranged on the driving part through the hand lifting part. The driving part is provided with a circumferential limiting assembly for driving the rotating drum to rotate, the circumferential limiting assembly is provided with a clamping assembly for limiting the axial movement of the rotating drum, the outer circumferential wall of the rotating drum is provided with a sleeving drum, the upper end of the stem cell separation bottle is provided with a quick release assembly, and the stem cell separation bottle is arranged on the sleeving drum in a sleeving mode through the quick release assembly. According to the stem cell separation bottle disclosed by the utility model, the stem cell separation bottle is convenient to disassemble and assemble through the circumferential limiting assembly, the clamping assembly and the quick disassembly assembly, so that the stem cell separation bottle is more tightly mounted.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell separation, and in particular to a separation device for bone marrow stem cell extraction. BACKGROUND

[0002] In the process of bone marrow stem cell extraction, the commonly used separation device is mainly a density gradient centrifugation device, and the most core part is a centrifuge, which is matched with special centrifuge tubes, separation liquid and other consumables to complete the separation work together, so as to extract the multipotent stem cells in the bone marrow. In the process of extracting cells from bone marrow, it is not difficult for long bones such as large animal long bones, but it is not easy to obtain bone marrow in the bone marrow cavity for small bones, especially for small animal bones.

[0003] At present, the Chinese utility model patent application with publication date of November 22, 2019 and publication number of CN 209669236U provides a mesenchymal stem cell separation and extraction device, which comprises a cylinder, a cover and a stem cell separation bottle. The top of the cylinder is provided with the cover, and a rotating motor and a rotating drum are arranged in the cylinder. A plurality of connecting columns are radially arranged on the outer wall of the rotating drum for mounting the stem cell separation bottle, and the free ends of the connecting columns extend obliquely downward. The stem cell separation bottle comprises a bottle cap, a tissue bottle body and a cell bottle body. The two ends of the tissue bottle body are connected with the bottle cap and the cell bottle body respectively. A filter screen is arranged at one end of the tissue bottle body connected with the cell bottle body. The rotating motor drives the rotating drum to rotate, and under the action of centrifugal force, the cells in the small bone tissue placed in the stem cell separation bottle pass through the filter screen and enter the cell bottle body from the tissue bottle body, while the small bone tissue is blocked by the filter screen and remains in the tissue bottle body, so that the cells are quickly and effectively separated from the small bone tissue. The structure is simple, the function is comprehensive, and the practicality is strong.

[0004] In the related art, the mesenchymal stem cell separation and extraction device uses a threaded connection between the bottle cap and the outer wall of the cylinder during use. However, since there is an included angle between the stem cell separation bottle and the cylinder, the included angle may cause unstable connection due to mismatched threads during threaded connection, which may lead to disconnection during centrifugation. Meanwhile, the cylinder and the device are in a non-separable state, which further causes unstable installation of the stem cell separation bottle.

[0005] Therefore, it is necessary to provide a separation device for bone marrow stem cell extraction to solve the above problems. CONTENT OF THE UTILITY MODEL

[0006] The embodiment of the present application provides a separation device for bone marrow stem cell extraction, in order to improve the technical problem that the separation bottle and the cylinder exist an included angle, the outer thread on the bottle cap and the inner thread on the cylinder can not be completely aligned in the screwing process, the cylinder is not detachable on the device, thereby causing the inaccurate cooperation between the threads, the centrifugal force can cause the thread to slip, and finally causing the separation bottle to be separated from the cylinder.

[0007] The embodiment of the present application provides a separation device for bone marrow stem cell extraction, comprising a rotating drum, a stem cell separation bottle arranged on the rotating drum, and a driving member for driving the rotating drum to rotate, a hand carrying member is arranged on the outer wall of the upper end of the rotating drum, the rotating drum is detachably arranged on the driving member through the hand carrying member, a circumferential limiting assembly for driving the rotating drum to rotate is arranged on the driving member, a clamping assembly for limiting the axial movement of the rotating drum is arranged on the circumferential limiting assembly, a sleeve connecting drum is arranged on the outer circumferential wall of the rotating drum, a quick release assembly is arranged on the upper end of the stem cell separation bottle, and the stem cell separation bottle is sleeved on the sleeve connecting drum through the quick release assembly, so that the stem cell separation bottle is convenient to disassemble and assemble.

[0008] The technical scheme described above in the embodiment of the present application has at least the following technical effects: in the process of using the separation device for bone marrow stem cell extraction, the rotating drum is taken off from the driving member through the hand carrying member, the stem cell separation bottle is sleeved on the sleeve connecting drum through the quick release assembly, then the rotating drum is placed on the driving member through the hand carrying member, and the driving member drives the rotating drum to rotate in the process of rotating, and the clamping assembly is used to limit the axial movement of the rotating drum in the process of rotating.

[0009] In the embodiment, the circumferential limiting assembly comprises a rotating disc arranged on the output shaft of the driving member, a connecting column arranged on the rotating disc, at least one limiting plate arranged on the side wall of the connecting column, and at least one limiting groove opened in the inner wall of the rotating drum and matched with the position of the limiting plate.

[0010] Through the technical scheme, when the rotating drum is sleeved on the driving member, the rotating drum is sleeved on the limiting plate through the limiting groove, the rotating drum is driven to rotate by the limiting plate and the limiting groove, the rotating drum abuts against the rotating disc and rotates synchronously, and the asynchronous rotation and slippage are avoided.

[0011] In the embodiment, a first connecting groove is opened at the abutment position of the limiting plate and the inner wall of the rotating drum, the clamping assembly comprises a first spring arranged in the first connecting groove and a first clamping block arranged at the end of the first spring away from the first connecting groove, and a first clamping groove is opened in the outer wall of the rotating drum and matched with the position of the first clamping block, so as to limit the axial movement of the rotating drum.

[0012] Through the technical scheme, in the process that the rotating drum is sleeved with the limiting groove and the limiting plate, the rotating drum compresses the first clamping block on the limiting plate, the first clamping block is compressed into the first connecting groove by the first spring, when the rotating drum abuts against the rotating disc, the first spring abuts against the first clamping block, the first clamping block is clamped in the first clamping groove, and the axial movement of the rotating drum is limited.

[0013] In the embodiment, the first clamping block is a right-angled trapezoid, and one end of the right angle of the first clamping block is close to the driving member.

[0014] Through the technical scheme, the shape of the right-angled trapezoid enables the first clamping block to be gradually clamped in the process of being compressed into the first connecting groove by the first spring, one end of the right angle close to the driving member enables the clamping block to smoothly enter the clamping groove through the inclined surface in the initial stage, and sudden clamping or jamming is avoided.

[0015] In the embodiment, the stem cell separation bottle comprises a bottle cap and a bottle body, at least one second connecting groove is formed in the bottle cap, the quick release assembly comprises a second spring arranged in the second connecting groove and a second clamping block arranged at an end of the second spring away from the second connecting groove, and an abutting groove for clamping the second clamping block is formed in the inner wall of the sleeving cylinder.

[0016] Through the technical scheme, in the process that the stem cell separation bottle is connected with the rotating drum, the bottle cap is sleeved on the sleeving cylinder, the second clamping block compresses the second spring in the sleeving process, the second clamping block is compressed into the second connecting groove, when the second clamping block moves into the abutting groove, the second spring rebounds to abut the second clamping block in the abutting groove, and the stem cell separation bottle and the rotating drum are installed.

[0017] In the embodiment, the second clamping block is a right-angled triangle, the abutting groove comprises a straight surface part and an inclined surface part, and the inclination angle of the inclined surface part is greater than the inclination angle of the inclined surface of the second clamping block.

[0018] Through the technical scheme, the second clamping block is clamped in the abutting groove, the straight surface part has an abutting force vertically upward on the second clamping block, in the process that the second clamping block is taken out of the abutting groove, the stem cell separation bottle is pushed inward, the inclined surface part compresses the second clamping block, the second clamping block is completely compressed into the connecting groove, and the stem cell separation bottle is taken out by rotating the stem cell separation bottle.

[0019] In the embodiment, the driving member is provided with a triangular damping member, the triangular damping member comprises a triangular connecting plate, supporting legs are arranged at the corners of the triangular connecting plate, guide columns are sleeved in the supporting legs, damping springs are sleeved outside the guide columns, one end of the damping spring abuts against the supporting leg, and the other end of the damping spring abuts against the triangular connecting plate.

[0020] Through the technical scheme, the damping spring can effectively absorb the vibration generated in the operation of the equipment through the elastic deformation of the damping spring, reduce the influence of the vibration on other components of the equipment, and improve the stability and reliability of the equipment.

[0021] In the embodiment, the bottle body comprises a tissue bottle body and a cell bottle body, two ends of the tissue bottle body are connected with a bottle cap and the cell bottle body respectively, and a filter screen is further arranged on the end of the tissue bottle body connected with the cell bottle body.

[0022] Through the technical scheme, the cells in the small bone tissue placed in the stem cell separation bottle pass through the filter screen and enter the cell bottle body from the tissue bottle body, and the small bone tissue is blocked by the filter screen and remains in the tissue bottle body, so that the cells are quickly and effectively separated from the small bone tissue.

[0023] In the embodiment, the included angle between the stem cell separation bottle and the rotating drum is not greater than 45°.

[0024] Through the technical scheme, the design that the included angle is not greater than 45° enables the stem cell separation bottle to be subjected to force more uniformly in the centrifugal process, the centrifugal force is reasonably distributed along the axial direction and the radial direction of the separation bottle, and the separation is not complete or the cells are damaged due to uneven force.

[0025] In the embodiment, a shell is arranged outside the device, a numerical control is arranged on the shell, and the driving member is electrically connected with the numerical control.

[0026] Through the technical scheme, the numerical control is arranged on the shell, so that an operator can centrally control various functions of the device through the control panel on the shell, the design of the centralized control simplifies the operation process, reduces the operation steps, and improves the convenience of operation. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 A perspective structural schematic view of the separation device for extracting bone marrow stem cells is provided in the embodiment of the application;

[0028] Figure 2 An exploded structural schematic view of the separation device for extracting bone marrow stem cells is provided in the embodiment of the application;

[0029] Figure 3A cross-sectional structure schematic view of the circumferential limiting assembly provided by the embodiment of the present application is shown in the figure;

[0030] Figure 4 A cross-sectional structure schematic view of the circumferential limiting assembly provided by the embodiment of the present application is shown in the figure; Figure 3 An enlarged view of A in the figure;

[0031] Figure 5 A cross-sectional structure schematic view of the stem cell separation bottle provided by the embodiment of the present application is shown in the figure;

[0032] Figure 6 A three-dimensional structure schematic view of the supporting leg provided by the embodiment of the present application is shown in the figure.

[0033] In the figure, various reference signs are as follows:

[0034] 1, housing; 11, numerical control; 12, rotating drum; 13, driving piece; 14, hand-held piece; 2, stem cell separation bottle; 21, bottle cap; 22, bottle body; 221, tissue bottle body; 222, cell bottle body; 223, filter screen; 3, circumferential limiting assembly; 31, rotating disc; 32, connecting column; 33, limiting plate; 34, limiting groove; 4, clamping assembly; 41, first connecting groove; 42, first spring; 43, first clamping block; 44, first clamping groove; 5, quick release assembly; 51, sleeving cylinder; 52, second connecting groove; 53, second spring; 54, second clamping block; 55, abutting groove; 551, straight face part; 552, inclined face part; 6, triangular damping piece; 61, triangular connecting plate; 62, supporting leg; 63, guide column; 64, damping spring. DETAILED DESCRIPTION

[0035] In the mesenchymal stem cell separation and extraction device in the related art, the connection between the bottle cap and the outer wall of the drum body is used by means of threaded connection, but since there is an included angle between the stem cell separation bottle and the drum body, the existence of the included angle causes the threaded connection to be unstable due to mismatching of the threads during threaded connection, and in the centrifugal process, the stem cell separation bottle can be separated, and the drum body and the device are in a state of inseparability, which further causes instability during installation of the stem cell separation bottle.

[0036] Therefore, in order to solve the technical problem that the outer thread on the bottle cap and the inner thread on the drum body can not be completely aligned during screwing due to the existence of an included angle between the separation bottle and the drum body in the related art, the drum body is not detachable from the device, thereby causing inaccurate cooperation between the threads, the centrifugal force can cause the threads to slip, and finally the separation bottle is separated from the drum body, the embodiments of the present application provide the following solutions.

[0037] Please refer to Figures 1 to 6The embodiment of the present application provides a separation device for bone marrow stem cell extraction, which comprises a rotating drum 12, a stem cell separation bottle 2 arranged on the rotating drum 12, and a driving member 13 for driving the rotating drum 12 to rotate, a hand-held member 14 arranged on the outer wall of the upper end of the rotating drum 12, the rotating drum 12 being detachably arranged on the driving member 13 through the hand-held member 14, a circumferential limiting assembly 3 arranged on the driving member 13 and used for driving the rotating drum 12 to rotate, a clamping assembly 4 arranged on the circumferential limiting assembly 3 and used for limiting the axial movement of the rotating drum 12, a sleeve 51 arranged on the outer circumferential wall of the rotating drum 12, and a quick-release assembly 5 arranged on the upper end of the stem cell separation bottle 2, the stem cell separation bottle 2 being sleeved on the sleeve 51 through the quick-release assembly 5, so that the stem cell separation bottle 2 is convenient to disassemble and assemble.

[0038] In the process of using the separation device for bone marrow stem cell extraction, the rotating drum 12 is taken off from the driving member 13 through the hand-held member 14, the stem cell separation bottle 2 is sleeved on the sleeve 51 through the quick-release assembly 5, and then the rotating drum 12 is placed on the driving member 13 through the hand-held member 14, and the driving member 13 drives the rotating drum 12 to rotate through the circumferential limiting assembly 3, and the clamping assembly 4 is used for limiting the axial movement of the rotating drum 12 in the process of rotation. In this way, the stem cell separation bottle 2 can be quickly sleeved on the sleeve 51 through the quick-release assembly 5, and the installation and disassembly process is simplified. This greatly reduces the time for equipment preparation and cleaning, improves the work efficiency, ensures that the rotating drum 12 can synchronously rotate in the process of rotation of the driving member 13, avoids the slipping or asynchronization between the rotating drum 12 and the driving member 13, limits the axial movement of the rotating drum 12 in the process of rotation through the clamping assembly 4, and further enhances the stability of the connection. The axial movement may cause the vibration of the equipment or the shaking of the separation bottle, affects the separation effect, and even causes a safety hazard.

[0039] In the embodiment, the circumferential limiting assembly 3 comprises a rotating disc 31 arranged on the output shaft of the driving member 13, a connecting column 32 arranged on the rotating disc 31, at least one limiting plate 33 arranged on the side wall of the connecting column 32, and at least one limiting groove 34 opened in the inner wall of the rotating drum 12 and matched with the position of the limiting plate 33.

[0040] In this way, when the rotating drum 12 is sleeved on the driving member 13, the rotating drum 12 is sleeved on the limiting plate 33 through the limiting groove 34, the rotating drum 12 is driven to rotate through the limiting plate 33 and the limiting groove 34, the rotating drum 12 abuts against the rotating disc 31 and synchronously rotates. In this way, the movement track of the rotating drum 12 is strictly limited through the cooperation of the limiting groove 34 and the limiting plate 33, and the rotating drum 12 is ensured to synchronously rotate with the driving member 13. This design avoids the slipping or asynchronization between the rotating drum 12 and the driving member 13, and guarantees the stability and reliability of the equipment in high-speed operation.

[0041] In the embodiment, the limiting plate 33 is provided with a first connecting groove 41 at the abutting position with the inner wall of the rotating drum 12, the clamping assembly 4 comprises a first spring 42 arranged in the first connecting groove 41 and a first clamping block 43 arranged at the end of the first spring 42 away from the first connecting groove 41, and the outer wall of the rotating drum 12 is provided with a first clamping groove 44 corresponding to the position of the first clamping block 43, so as to limit the axial movement of the rotating drum 12.

[0042] In this way, during the sleeving of the rotating drum 12 and the limiting plate 33 through the limiting groove 34, the first clamping block 43 on the limiting plate 33 is compressed by the rotating drum 12, the first clamping block 43 is compressed into the first connecting groove 41 by the first spring 42, when the rotating drum 12 abuts against the rotating disc 31, the first spring 42 abuts against the first clamping block 43, the first clamping block 43 is clamped in the first clamping groove 44, and the axial movement of the rotating drum 12 is limited. In this way, the first clamping block 43 can be accurately clamped in the first clamping groove 44 under the action of the first spring 42, the axial movement of the rotating drum 12 during rotation is prevented, reliable axial limiting is provided, and the stability and accuracy of the equipment during high-speed operation are ensured.

[0043] In the embodiment, the first clamping block 43 is a right-angled trapezoid, and the right angle end of the first clamping block 43 is close to the driving member 13.

[0044] In this way, the shape of the right-angled trapezoid enables the first clamping block 43 to realize gradual clamping during the compression of the first spring 42 and the entering of the first connecting groove 41, and the right angle end close to the driving member 13 enables the clamping block to smoothly enter the clamping groove through the inclined surface in the initial stage, so as to avoid sudden clamping or jamming.

[0045] In the embodiment, the stem cell separation bottle 2 comprises a bottle cap 21 and a bottle body 22, the bottle cap 21 is provided with at least one second connecting groove 52, the quick release assembly 5 comprises a second spring 53 arranged in the second connecting groove 52 and a second clamping block 54 arranged at the end of the second spring 53 away from the second connecting groove 52, and the inner wall of the sleeving cylinder 51 is provided with an abutting groove 55 for clamping the second clamping block 54.

[0046] In this way, during the connection of the stem cell separation bottle 2 and the drum 12, the bottle cap 21 is sleeved on the sleeve sleeve 51, and the second clamping block 54 is compressed to the second connecting groove 52 by the second spring 53 during the sleeving process. When the second clamping block 54 moves to the abutting groove 55, the second spring 53 rebounds to abut the second clamping block 54 in the abutting groove 55, and the stem cell separation bottle 2 and the drum 12 are installed. In this way, the design of the second clamping block 54 and the second spring 53 enables the stem cell separation bottle 2 to be automatically aligned with the sleeve sleeve 51 during the sleeving process, reducing the difficulty and operation time of manual alignment. Due to the automatic rebounding action of the second spring 53, the operator does not need to use additional tools or components for installation, further simplifying the operation process and improving the work efficiency.

[0047] In this embodiment, the second clamping block 54 is a right-angled triangle, and the abutting groove 55 includes a straight face part 551 and an inclined face part 552. The inclination angle of the inclined face part 552 is greater than that of the inclined face of the second clamping block 54.

[0048] In this way, the second clamping block 54 is clamped inside the abutting groove 55, and the straight face part 551 has an upward abutting force on the second clamping block 54. During the process of removing the second clamping block 54 from the abutting groove 55, the stem cell separation bottle 2 is pushed inward, the inclined face part 552 compresses the second clamping block 54, and the second clamping block 54 is completely compressed into the connecting groove. By rotating the stem cell separation bottle 2, the entire stem cell separation bottle 2 is removed. In this way, the entire removal process is divided into two clear steps: first, the separation bottle is pushed inward to compress the clamping block, and then the separation bottle is rotated to complete the removal. During normal operation, the upward abutting force of the straight face part 551 on the second clamping block 54 ensures the stability of the clamping block, preventing it from accidentally falling off during high-speed centrifugation or vibration, and improving the safety of the equipment.

[0049] In this embodiment, the driving member 13 is provided with a triangular damping member 6, which includes a triangular connecting plate 61. The triangular connecting plate 61 is provided with a support foot 62 at the corner thereof. The support foot 62 is sleeved with a guide column 63, and the guide column 63 is sleeved with a damping spring 64. One end of the damping spring 64 abuts against the support foot 62, and the other end of the damping spring 64 abuts against the triangular connecting plate 61.

[0050] In this way, the damping spring 64 can effectively absorb the vibration generated during the operation of the equipment by its elastic deformation, reduce the influence of vibration on other parts of the equipment, and improve the stability and reliability of the equipment. Vibration is one of the important factors that cause equipment parts to wear. Through the damping effect of the damping spring 64, the vibration generated during the operation of the equipment can be significantly reduced, thereby reducing mechanical wear and prolonging the service life of the equipment.

[0051] In the embodiment, the bottle body 22 comprises a tissue bottle body 221 and a cell bottle body 222, two ends of the tissue bottle body 221 are connected with the bottle cap 21 and the cell bottle body 222 respectively, and a filter screen 223 is further arranged on the end of the tissue bottle body 221 connected with the cell bottle body 222.

[0052] In this way, the cells in the small bone tissue placed in the stem cell separation bottle 2 pass through the filter screen 223 and enter the cell bottle body 222 from the tissue bottle body 221, while the small bone tissue is blocked by the filter screen 223 and remains in the tissue bottle body 221, so that the cells are quickly and effectively separated from the small bone tissue. In this way, the design of the filter screen 223 enables the cells to quickly pass through the filter screen 223 and enter the cell bottle body 222, thereby realizing the rapid separation of the cells from the bone tissue. This efficient separation process can significantly shorten the experimental time and improve the work efficiency.

[0053] In the embodiment, the included angle between the stem cell separation bottle 2 and the drum 12 is not greater than 45°.

[0054] In this way, the design of the included angle not greater than 45° enables the stem cell separation bottle 2 to be subjected to force more uniformly during centrifugation, and the centrifugal force is reasonably distributed along the axial and radial directions of the separation bottle, thereby avoiding incomplete separation or cell damage caused by uneven force.

[0055] In the embodiment, the device is externally provided with a shell 1, the shell 1 is provided with a numerical control 11, and the driving member 13 is electrically connected with the numerical control 11.

[0056] In this way, the numerical control 11 is arranged on the shell 1, so that the operator can centrally control various functions of the device through the control panel on the shell 1. This centralized control design simplifies the operation process, reduces the operation steps, and improves the convenience of operation.

[0057] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A separation device for extracting bone marrow stem cells, comprising a rotating drum (12), a stem cell separation bottle (2) arranged on the rotating drum (12), and a driving member (13) for driving the rotating drum (12) to rotate, characterized in that: A hand piece (14) is provided on the outer wall of the upper end of the rotating drum (12), and the rotating drum (12) is detachably provided on the driving member (13) through the hand piece (14). The driving member (13) is provided with a circumferential limiting component (3) for driving the rotating drum (12) to rotate, and the circumferential limiting component (3) is provided with a clamping component (4) for limiting the axial movement of the rotating drum (12). A sleeve tube (51) is provided on the outer peripheral wall of the rotating drum (12), and a quick-release component (5) is provided on the upper end of the stem cell separation bottle (2). The stem cell separation bottle (2) is sleeved on the sleeve tube (51) through the quick-release component (5), so as to facilitate the disassembly and assembly of the stem cell separation bottle (2).

2. The separation device for extracting bone marrow stem cells according to claim 1, characterized in that: The circumferential limiting assembly (3) comprises a rotating disk (31) arranged on the output shaft of the driving member (13), a connecting column (32) arranged on the rotating disk (31), at least one limiting plate (33) arranged on the side wall of the connecting column (32), and at least one limiting groove (34) provided on the inner wall of the rotating drum (12) and matching the position of the limiting plate (33).

3. The separation device for extracting bone marrow stem cells according to claim 2, characterized in that: A first connecting groove (41) is provided at a position where the limiting plate (33) abuts against the inner wall of the rotating drum (12); the clamping assembly (4) comprises a first spring (42) arranged inside the first connecting groove (41), and a first clamping block (43) arranged at an end of the first spring (42) away from the first connecting groove (41); a first clamping groove (44) is provided on the outer wall of the rotating drum (12) at a position corresponding to the position of the first clamping block (43) to limit the axial movement of the rotating drum (12).

4. The separation device for extracting bone marrow stem cells according to claim 3, characterized in that: The first clamping block (43) is a right-angled trapezoid, and one right-angled end of the first clamping block (43) is close to the driving member (13).

5. The separation device for extracting bone marrow stem cells according to claim 1, characterized in that: The stem cell separation bottle (2) comprises a bottle cap (21) and a bottle body (22), wherein the bottle cap (21) is provided with at least one second connecting groove (52), the quick-release assembly (5) comprises a second spring (53) arranged in the second connecting groove (52), and a second clamping block (54) arranged at one end of the second spring (53) away from the second connecting groove (52), and an abutting groove (55) for the second clamping block (54) to be clamped is provided on the inner wall of the sleeve (51).

6. The separation device for extracting bone marrow stem cells according to claim 5, characterized in that: The second clamping block (54) is in the shape of a right-angled triangle, and the abutting groove (55) comprises a straight surface portion (551) and an inclined surface portion (552), wherein the inclination angle of the inclined surface portion (552) is greater than the inclination angle of the inclined surface in the second clamping block (54).

7. A separation device for extracting bone marrow stem cells according to claim 1, 2, 3, 4, 5 or 6, characterized in that: A triangular shock-absorbing member (6) is provided on the driving member (13), and the triangular shock-absorbing member (6) includes a triangular connecting plate (61). The triangular connecting plate (61) is provided with a supporting foot (62) at a corner thereof. A guide column (63) is provided inside the supporting foot (62), and a shock-absorbing spring (64) is provided outside the guide column (63). One end of the shock-absorbing spring (64) abuts against the supporting foot (62), and the other end of the shock-absorbing spring (64) abuts against the triangular connecting plate (61).

8. The separation device for extracting bone marrow stem cells according to claim 5, characterized in that: The bottle body (22) comprises a tissue bottle body (221) and a cell bottle body (222), the two ends of the tissue bottle body (221) being connected to the bottle cap (21) and the cell bottle body (222), respectively, and a filter screen (223) is further provided on the end of the tissue bottle body (221) connected to the cell bottle body (222).

9. The separation device for extracting bone marrow stem cells according to claim 1, characterized in that: The angle between the stem cell separation bottle (2) and the rotating drum (12) is no greater than 45°.

10. A separation device for extracting bone marrow stem cells according to claim 1 or 2 or 3 or 4 or 5 or 6 or 8 or 9, characterized in that: A housing (1) is provided outside the device, a digital control unit (11) is provided on the housing (1), and the driving component (13) is electrically connected to the digital control unit (11).

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