A micro-PCR reaction auxiliary device
By designing a micro-PCR reaction auxiliary device, the problems of prolonged reaction time and poor uniformity caused by large PCR reaction tube capacity are solved, realizing rapid and efficient PCR reaction, compatible with ordinary PCR instruments, saving reagent consumption and reducing costs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHANGHAI JIENUO BIOLOGICAL TECH CO LTD
- Filing Date
- 2025-06-06
- Publication Date
- 2026-06-26
AI Technical Summary
Existing PCR reaction tubes have large capacities, which leads to prolonged reaction times and poor uniformity. Furthermore, ordinary PCR instruments cannot be directly adapted to micro-scale reaction systems.
Design a micro-PCR reaction auxiliary device, including a conical core, a partition, a connecting rod, a threaded connector, a screw structure, a pressing part, a fixing plate, and a contact rod. By using these components in combination, the PCR reaction system liquid is distributed near the tube wall, improving heat conduction efficiency and fixing it inside the PCR reaction tube, and is compatible with ordinary PCR instruments.
It improves the speed and uniformity of PCR reactions, reduces the amount of reaction reagents used, eliminates the need for additional equipment purchases, lowers costs, and is compatible with ordinary PCR reaction tubes.
Smart Images
Figure CN224411755U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of medical testing device technology, specifically to a micro-PCR reaction auxiliary device. Background Technology
[0002] PCR (Polymerase Chain Reaction) is a technique used to amplify specific DNA fragments in vitro. With the development of PCR technology, new techniques such as fluorescent PCR, digital PCR, and microfluidic PCR are constantly being developed. These techniques play an increasingly important role in applications such as infectious disease diagnosis, genetic disease diagnosis, and tumor diagnosis.
[0003] Currently, ordinary PCR reaction tubes typically have a capacity of 0.1 ml to 0.5 ml, are tubular in shape, and usually have a conical bottom. Their shape perfectly matches the heating module in the PCR instrument. Temperature changes in the heating module of the PCR instrument are conducted to the reaction system in the PCR reaction tube through the PCR reaction tube, triggering the PCR reaction. Since the ordinary reaction system usually requires 20-50 μL, it takes a certain amount of time for the reaction system to reach equilibrium when the temperature changes, resulting in prolonged reaction time and poor reaction uniformity.
[0004] Based on this, the present invention designs a micro-PCR reaction auxiliary device to solve the above problems. Utility Model Content
[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a micro-PCR reaction auxiliary device.
[0006] To achieve the above objectives, this utility model provides the following technical solution: a micro-PCR reaction auxiliary device, comprising a conical core, partitions, connecting rods, a ring frame, a threaded connector, a screw structure, a pressing part, a fixing plate, a contact rod, and a PCR reaction tube. The conical core is a hollow tubular structure with a conical bottom. Four partitions are fixedly connected to the outer side of the conical core. The upper and lower edges of the partitions are chamfered. Four connecting rods are fixedly connected to the upper end of the conical core. The top ends of the four connecting rods are fixedly connected to the ring frame. A threaded connector is fixedly connected to the center of the ring frame. A screw structure is spirally connected inside the threaded connector.
[0007] As a preferred embodiment of this utility model, the lower end of the screw structure is movably connected to the pressing part, the pressing part is slidably connected to four connecting rods, a fixing plate is provided directly below the pressing part, the fixing plate is fixedly connected to the four connecting rods, and four abutting rods are movably connected inside the fixing plate.
[0008] As a preferred embodiment of this utility model, the screw structure includes a rod body, a rotating rod, and a limiting block. The rod body is helically connected to a threaded connection seat, the rotating rod is fixedly connected to the top end of the rod body, and the limiting block is fixedly connected to the bottom end of the rod body.
[0009] As a preferred technical solution of this utility model, the pressing part includes a movable sliding plate and a trapezoidal block. The movable sliding plate has grooves around its perimeter that match the connecting rod. A trapezoidal block is provided at the center of the lower end of the movable sliding plate. The trapezoidal block is in the shape of a flat-topped pyramid.
[0010] As a preferred embodiment of this utility model, the limiting block at the lower end of the screw structure is movably connected to the movable sliding plate of the pressing part.
[0011] As a preferred technical solution of this utility model, the fixing plate includes a plate body, a trapezoidal groove and a guide groove. The plate body is fixedly connected to the connecting rod. The trapezoidal groove is provided at the center of the plate body. Through grooves are provided around the plate body, and guide grooves are symmetrically provided at the points where the through grooves and the trapezoidal grooves connect.
[0012] As a preferred embodiment of this utility model, guide blocks are symmetrically arranged on both sides of the abutment rod, the abutment rod is movably disposed in the through groove opened on the plate, and the guide blocks on the abutment rod are slidably connected to the guide groove.
[0013] As a preferred embodiment of this utility model, the partitions around the conical tube core are in contact with the inner side of the PCR reaction tube, and the four abutting rods around the fixing plate are fixed in contact with the inside of the PCR reaction tube.
[0014] Compared with the prior art, the beneficial effects of this utility model are:
[0015] 1. When in use, this device, in conjunction with a PCR reaction tube, distributes the liquid in the PCR reaction system near the tube wall, increasing the conduction efficiency with the PCR instrument's thermal module, thus improving the reaction speed. At the same time, it avoids the process of internal heat conduction in the liquid, improving the uniformity of the reaction system and increasing the PCR reaction efficiency. The overall volume of the reaction system can be reduced from 20-50 μL to 1-5 μL, greatly saving the amount of reaction reagents and the sample volume requirements. Furthermore, this device is compatible with common PCR reaction tubes available on the market. Using this device requires no additional instruments or consumables and will not increase additional costs.
[0016] 2. When using this utility model, after placing the entire device into the PCR reaction tube, the partition on the outside of the conical tube core comes into contact with the inside of the conical tube core. Then, a tool is used to turn the rotating rod to rotate the rod body. By turning the rod body downward, the pressing part is pushed down, causing the trapezoidal block to be pressed into the trapezoidal groove. This squeezes and limits the four contact rods, making the four contact rods contact and fix them against the inner wall of the PCR reaction tube. This gives the device good stability inside the PCR reaction tube and makes it less likely to move. Attached Figure Description
[0017] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:
[0018] Figure 1 This is a schematic diagram of the overall front view of the present invention;
[0019] Figure 2 This is a schematic cross-sectional view of the overall structure of this utility model;
[0020] Figure 3 This is a partially enlarged cross-sectional view of the present invention.
[0021] Figure 4 This is a schematic diagram of the overall structure of the lower pressing part of this utility model;
[0022] Figure 5 This is a cross-sectional view of the fixing plate of this utility model;
[0023] Figure 6 This is a schematic diagram of the overall installation and use structure of this utility model.
[0024] In the diagram: 1. Conical core; 2. Partition plate; 3. Connecting rod; 4. Ring frame; 5. Threaded connector; 6. Screw structure; 601. Rod body; 602. Rotary rod; 603. Limiting block; 7. Pressing part; 701. Moving slide plate; 702. Trapezoidal block; 8. Fixing plate; 801. Plate body; 802. Trapezoidal groove; 803. Guide groove; 9. Abutment rod; 901. Guide block; 10. PCR reaction tube. Detailed Implementation
[0025] The following will refer to the appendix in the embodiments of this utility model. Figure 1-6 The technical solutions in the embodiments of this utility model are clearly and completely described herein. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.
[0026] Example
[0027] Please see Figure 1-6 The present invention provides the following technical solution: a micro-PCR reaction auxiliary device, comprising a conical core 1, a partition 2, a connecting rod 3, a ring frame 4, a threaded connecting seat 5, a screw structure 6, a pressing part 7, a fixing plate 8, a contact rod 9, and a PCR reaction tube 10. The conical core 1 is a hollow tubular structure with a conical bottom. Four partitions 2 are fixedly connected to the outside of the conical core 1. The upper and lower edges of the partitions 2 are chamfered. Four connecting rods 3 are fixedly connected to the upper end of the conical core 1. The top ends of the four connecting rods 3 are fixedly connected to the ring frame 4. A threaded connecting seat 5 is fixedly connected to the center of the ring frame 4. A screw structure 6 is spirally connected inside the threaded connecting seat 5.
[0028] The lower end of the screw structure 6 is movably connected to the pressing part 7. The pressing part 7 is slidably connected to the four connecting rods 3. A fixing plate 8 is provided directly below the pressing part 7. The fixing plate 8 is fixedly connected to the four connecting rods 3. Four abutting rods 9 are movably connected inside the fixing plate 8.
[0029] Through the above structural design, the device can restrict the liquid distribution in the reaction system to the vicinity of the tube wall during use, thereby improving the uniformity of the reaction system, increasing the efficiency of PCR reaction, and greatly saving the amount of reaction reagents and the requirements for sample quantity.
[0030] The screw structure 6 includes a rod body 601, a rotating rod 602, and a limiting block 603. The rod body 601 is screwed to the threaded connection seat 5. The rotating rod 602 is fixedly connected to the top end of the rod body 601, and the limiting block 603 is fixedly connected to the bottom end of the rod body 601.
[0031] The pressing part 7 includes a movable slide plate 701 and a trapezoidal block 702. The movable slide plate 701 has grooves around its perimeter that match the connecting rod 3. The trapezoidal block 702 is located at the center of the lower end of the movable slide plate 701. The trapezoidal block 702 is a flat-topped pyramid.
[0032] The limiting block 603 at the lower end of the screw structure 6 is movably connected to the movable slide plate 701 of the pressing part 7.
[0033] The fixing plate 8 includes a plate body 801, a trapezoidal groove 802 and a guide groove 803. The plate body 801 is fixedly connected to the connecting rod 3. The trapezoidal groove 802 is provided at the center of the plate body 801. Through grooves are provided around the plate body 801, and guide grooves 803 are symmetrically provided at the points where the through grooves and the trapezoidal groove 802 connect.
[0034] Guide blocks 901 are symmetrically arranged on both sides of the abutment rod 9. The abutment rod 9 is movably arranged in the through groove opened on the plate 801, and the guide blocks 901 on the abutment rod 9 are slidably connected to the guide groove 803.
[0035] Through the movable connection structure between the fixing plate 8 and the abutment rod 9, the four abutment rods 9 are squeezed during the downward movement of the pressing part 7, so that the four abutment rods 9 are fixed against the inner wall of the PCR reaction tube 10, thereby improving the stability during use.
[0036] The partitions 2 around the conical tube core 1 are in contact with the inside of the PCR reaction tube 10, and the four abutting rods 9 around the fixing plate 8 are in contact with and fixed to the inside of the PCR reaction tube 10.
[0037] After the entire device is placed into the PCR reaction tube 10, the partitions 2 around the conical core 1 and the abutment rods 9 around the fixing plate 8 are in contact with the inner wall of the PCR reaction tube 10.
[0038] The working principle and usage process of this utility model are as follows: In specific use, the device can first be placed into an empty PCR reaction tube 10, so that a cavity is formed between the inner wall of the tube and the reaction tube. Then, the prepared PCR reaction system is added into the reaction cavity. After closing the cap, it is placed in a PCR instrument for reaction. Alternatively, the prepared PCR reaction system can be added into an empty PCR reaction tube 10 first, and then the device can be placed in it, controlling the PCR reaction system to the tube wall. After closing the cap, it is placed in a PCR instrument for reaction. By using a tool to turn the rotating rod 602 to drive the rod body 601 to rotate, the downward rotation of the rod body 601 causes the trapezoidal block 702 to be pressed into the trapezoidal groove 802. At the same time, the four abutting rods 9 are squeezed outward, so that the abutting rods 9 are in contact with and fixed to the inner wall of the PCR reaction tube 10, thus fixing the entire device and preventing the device from moving in the PCR reaction tube 10. This device can be adapted to a single PCR reaction tube, as well as multi-tube or multi-well plate and other PCR consumables.
[0039] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.
Claims
1. A micro-PCR reaction auxiliary device, characterized in that: The device includes a conical core (1), a partition (2), a connecting rod (3), a ring frame (4), a threaded connector (5), a screw structure (6), a pressing part (7), a fixing plate (8), a contact rod (9), and a PCR reaction tube (10). The conical core (1) is a hollow tubular structure with a conical bottom. Four partitions (2) are fixedly connected to the outside of the conical core (1). The upper and lower edges of the partitions (2) are chamfered. Four connecting rods (3) are fixedly connected to the upper end of the conical core (1). The top ends of the four connecting rods (3) are fixedly connected to the ring frame (4). A threaded connector (5) is fixedly connected to the center of the ring frame (4). A screw structure (6) is spirally connected inside the threaded connector (5).
2. The micro-PCR reaction auxiliary device according to claim 1, characterized in that: The lower end of the screw structure (6) is movably connected to the pressing part (7), the pressing part (7) is slidably connected to four connecting rods (3), a fixing plate (8) is provided directly below the pressing part (7), the fixing plate (8) is fixedly connected to the four connecting rods (3), and four abutting rods (9) are movably connected inside the fixing plate (8).
3. The micro-PCR reaction auxiliary device according to claim 1, characterized in that: The screw structure (6) includes a rod body (601), a rotating rod (602), and a limiting block (603). The rod body (601) is screwed to the threaded connection seat (5). The rotating rod (602) is fixedly connected to the top of the rod body (601), and the limiting block (603) is fixedly connected to the bottom of the rod body (601).
4. The micro-PCR reaction auxiliary device according to claim 1, characterized in that: The pressing part (7) includes a movable slide plate (701) and a trapezoidal block (702). The movable slide plate (701) has a groove around its perimeter that matches the connecting rod (3). A trapezoidal block (702) is provided at the center of the lower end of the movable slide plate (701). The trapezoidal block (702) is in the shape of a flat-topped pyramid.
5. The micro-PCR reaction auxiliary device according to claim 1, characterized in that: The limiting block (603) at the lower end of the screw structure (6) is movably connected to the movable slide plate (701) of the pressing part (7).
6. The micro-PCR reaction auxiliary device according to claim 1, characterized in that: The fixing plate (8) includes a plate body (801), a trapezoidal groove (802) and a guide groove (803). The plate body (801) is fixedly connected to the connecting rod (3). The trapezoidal groove (802) is provided at the center of the plate body (801). Through grooves are provided around the plate body (801), and guide grooves (803) are symmetrically provided at the connection points between the through grooves and the trapezoidal groove (802).
7. The micro-PCR reaction auxiliary device according to claim 1, characterized in that: Guide blocks (901) are symmetrically arranged on both sides of the abutment rod (9). The abutment rod (9) is movably arranged in the through groove opened on the plate (801). The guide blocks (901) on the abutment rod (9) are slidably connected to the guide groove (803).
8. The micro-PCR reaction auxiliary device according to claim 1, characterized in that: The partition (2) around the conical core (1) contacts the inside of the PCR reaction tube (10), and the four abutting rods (9) around the fixing plate (8) abut against the inside of the PCR reaction tube (10) for fixation.