Intestinal flora culture well plate structure

Abstract

This utility model discloses a well plate structure for intestinal flora culture, belonging to the field of flora culture experimental technology. It includes a culture plate and multiple culture containers. The culture plate has a placement hole that matches the shape of the bottom end of the culture container. The outer side wall of the bottom end of the culture container and the inner side wall of the placement hole are fixed by threaded engagement. The top of the culture container is provided with a detachable top cover. The center of the top cover has a puncture hole for the intestinal flora extraction syringe to enter and exit. An extension tube is connected to the puncture hole. The bottom end of the extension tube is frustoconical and the inner wall is provided with a silicone layer. The bottom of the culture container is provided with a plug that matches the opening of the bottom end of the extension tube. By utilizing the sealing fit between the top cover and the plug and the detachable design, the anaerobic environment required for intestinal flora culture is ensured, while avoiding the puncture problem caused by multiple layers of sealing.

Description

Technical Field

[0001] This utility model relates to the field of bacterial culture experimental technology, and in particular to a well plate structure for intestinal bacterial culture. Background Technology

[0002] In the field of gut microbiota research, gut microbiota culture is a crucial step. Gut microbiota refers to the bacterial community that resides in the human gut and coexists with the human body for a long time. In the specific experimental operation of gut microbiota culture, culture plates are a commonly used tool, and their performance has an important impact on the experimental results. The prior art patent with publication number CN215757347U discloses a rapid determination device for evaluating the regulatory function of gut microbiota. It includes a microbiota culture plate with at least one culture well. The culture well is open at the top and closed at the bottom. An elastic adhesive layer is pasted and covered on the upper surface of the microbiota culture plate. The lower surface of the elastic adhesive layer is sealed to the upper surface of the microbiota culture plate, so that each culture well is sealed. This solves the problems of large workload, long time consumption, poor repeatability of parallel samples, and difficulty in controlling the anaerobic environment required by gut microbiota in batch analysis. It realizes rapid in vitro fermentation of gut microbiota and high-throughput screening of samples.

[0003] The elastic adhesive layer in this patent application is made of a rubber film with a strong adhesive coating on the lower surface and high elasticity. It is used to adhere and cover the upper surface of the bacterial culture plate, thereby sealing each culture well. During the bacterial culture process, the sampler needs to pass through the elastic adhesive layer to sample the culture well. After each sampling, another layer of elastic adhesive is added to achieve resealing. However, in the operation of this patent application, as the thickness of the elastic adhesive layer increases, the difficulty of syringe puncture increases significantly. Experimenters are prone to puncturing the device due to excessive force, resulting in experimental failure and poor overall performance. Utility Model Content

[0004] This invention provides a well plate structure for intestinal flora culture to solve the aforementioned technical problems.

[0005] The present invention adopts the following technical solution: it includes a culture plate; a plurality of placement holes are arranged on the culture plate, and a culture container is disposed in each placement hole area; the bottom shape of the culture container matches the shape of the placement hole on the culture plate, and the bottom part of the culture container can be placed exactly in the placement hole of the culture plate; the outer side wall of the bottom end of the culture container is provided with threads, and the inner side wall of each placement hole on the culture plate is provided with a threaded groove that matches the thread on the outer side wall of the culture container; the interior of the culture container is provided with a chamber, and the chamber area extends out from the top of the culture container; the top of the culture container is provided with a detachable top cover that can cover the chamber area.

[0006] Preferably, the top cover has a puncture hole at its center that extends through its top and bottom ends. This puncture hole is used to allow the bacterial extraction syringe to enter the culture container chamber for bacterial inoculation or sampling. The top cover has an extension tube at the location of the puncture hole, which is the same size as the puncture hole. The extension tube extends through both ends, and the bottom end of the extension tube extends a certain distance towards the lower part of the top cover.

[0007] Preferably, the tube body of the extension tube located below the top cover gradually expands outward from top to bottom, so that the external shape of the extension tube located below the top cover forms a frustum shape, and a silicone layer is fixedly provided on the inner wall of the bottom opening of the extension tube.

[0008] Preferably, a stopper is provided at the center of the bottom of the culture container, and the bottom end of the stopper is fixedly connected to the bottom of the culture container.

[0009] Preferably, the top of the plug is designed as a spherical structure, the shape and size of which match the shape of the bottom opening of the extension tube below the top cover, and the spherical part at the top of the plug can be inserted into the bottom opening of the extension tube and form a sealing state for the bottom of the extension tube.

[0010] Preferably, a guide groove is provided on the inner wall of the top of the culture container. The upper half of the guide groove is designed with a vertical trajectory, and the lower half of the guide groove is designed with a horizontal trajectory. The bottom height of the upper vertical part of the guide groove matches the height of the top of the extension tube below the top cover that is fitted onto the spherical part of the plug tip. A slider matching the width of the guide groove is fixedly provided on the outer wall of the top cover. When the top cover is inserted into the chamber area of ​​the culture container, it can drive the slider to move within the guide groove.

[0011] Preferably, the top end of the extension tube extends a certain distance towards the upper part of the top cover, and the extension tube located above the top cover and the extension tube located below the top cover have the same shape and are designed symmetrically. That is, the tube body of the extension tube above the top cover gradually expands outward from bottom to top, so that the external shape of the extension tube located above the top cover forms an inverted frustum shape. The top cover is provided with a lever plate on both sides of the extension tube, and the lever plate is provided with a slot. The lever plate is fixedly connected to the top cover and the extension tube.

[0012] The above-mentioned technical solutions adopted in the embodiments of this utility model can achieve the following beneficial effects:

[0013] Firstly, compared to existing technologies that rely on elastic adhesive layers for sealing, and where the difficulty of puncture increases dramatically after multiple samplings due to the thickening of the adhesive layer, this invention features a detachable top cover design. During inoculation or sampling, the top cover can be directly removed or the procedure can be performed through the puncture hole, eliminating the need for repeated layering of the sealing layer. Simultaneously, the lever structure of the top cover and the cooperation between the slider and guide groove make the installation, rotation, and lifting of the top cover easier, preventing experimental failures caused by excessive force breaking the device, and significantly improving operational safety.

[0014] Secondly, the culture container and the top cover are sealed by the cooperation of the plug and the extension tube. The silicone layer at the bottom of the extension tube is in close contact with the ball at the top of the plug, which can effectively seal the gap and ensure the anaerobic environment required for culture. Attached Figure Description

[0015] The accompanying drawings, which are included to provide a further understanding of the present invention and constitute a part of this invention, illustrate exemplary embodiments of the present invention and, together with the description thereof, serve to explain the present invention and do not constitute an undue limitation thereof. In the drawings:

[0016] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0017] Figure 2 This is a partial structural schematic diagram of the present invention;

[0018] Figure 3 This is a schematic diagram showing the disassembled culture container and top cover of this utility model;

[0019] Figure 4 This is a schematic diagram of the extension tube in this utility model;

[0020] Figure 5 This is a schematic diagram of the internal structure of the culture container in this utility model;

[0021] Figure 6 This is a schematic diagram of the guide groove in this utility model.

[0022] Figure Labels

[0023] Culture plate 1, well 11

[0024] Culture container 2, plug column 21, guide groove 22,

[0025] Top cover 3, extension tube 31, silicone layer 32, slider 33, dial plate 34. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be clearly and completely described below in conjunction with specific embodiments and corresponding drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0027] The technical solutions provided by the various embodiments of this utility model are described in detail below with reference to the accompanying drawings.

[0028] This utility model provides a well plate structure for intestinal flora culture, including a culture plate 1; the culture plate 1 has a plurality of placement holes 11 arranged on it, and a culture container 2 is disposed in the area of ​​each placement hole 11; the bottom shape of the culture container 2 matches the shape of the placement hole 11 on the culture plate 1, and the bottom part of the culture container 2 can be placed exactly in the placement hole 11 of the culture plate 1; the outer side wall of the bottom end of the culture container 2 is provided with threads, and the inner side wall of each placement hole 11 on the culture plate 1 is provided with a threaded groove that matches the thread on the outer side wall of the culture container 2; the interior of the culture container 2 is provided with a chamber, and the chamber area extends out from the top of the culture container 2; the top of the culture container 2 is provided with a detachable top cover 3 that can cover the chamber area;

[0029] The culture plate 1 can be used to place and transport multiple culture containers 2. When the culture container 2 is placed in the corresponding placement hole 11 on the culture plate 1, the culture container 2 can be rotated slightly so that the thread on the outer side wall of the bottom end of the culture container 2 is screwed into the thread groove on the inner wall of the placement hole 11 and forms a threaded engagement state. This can prevent the culture container 2 from falling out of the placement hole 11 when the entire culture plate 1 is moved.

[0030] The top cover 3 of the culture container 2 is designed to be detachable. When it is necessary to inoculate or sample the microbial community into the culture container 2, the top cover 3 can be removed to expose the chamber area of ​​the culture container 2 so that the microbial community extraction syringe can be inserted for operation.

[0031] In a further preferred embodiment, a puncture hole penetrating from the top and bottom of the top cover 3 is provided at the center. This puncture hole is used for the bacterial extraction syringe to enter the chamber of the culture container 2 to realize bacterial inoculation or sampling operations. An extension tube 31 matching the size of the puncture hole is provided on the top cover 3 at the location of the puncture hole. The extension tube 31 is provided through at both ends, and the bottom end of the extension tube 31 extends a certain distance towards the lower part of the top cover 3. The tube body of the extension tube 31 located below the top cover 3 gradually expands outward from top to bottom, so that the external shape of the extension tube 31 located below the top cover 3 forms a frustum shape. A silicone layer 32 is fixedly provided on the inner wall of the bottom opening of the extension tube 31.

[0032] In a further preferred embodiment, a plug 21 is provided at the center of the bottom of the culture container 2, and the bottom end of the plug 21 is fixedly connected to the bottom of the culture container 2; the top end of the plug 21 is designed as a spherical structure, and the shape and size of the sphere matches the shape of the bottom opening of the extension tube 31 below the top cover 3, and the spherical part of the top end of the plug 21 can be inserted into the bottom opening of the extension tube 31 and form a sealing state for the bottom end of the extension tube 31; a guide groove 22 is provided on the inner wall of the top of the culture container 2, the upper half of the guide groove 22 is designed as a vertical trajectory, and the lower half of the guide groove 22 is designed as a horizontal trajectory, and the height of the bottom of the vertical part of the upper half of the guide groove 22 matches the height of the top end of the extension tube 31 below the top cover 3 fitted onto the spherical part of the top end of the plug 21;

[0033] The outer wall of the top cover 3 is fixedly provided with a slider 33 that matches the width of the guide groove 22. When the top cover 3 is inserted into the chamber area of ​​the culture container 2, the slider 33 can be driven to move within the guide groove 22.

[0034] The prior art patent with publication number CN215757347U describes a rapid assay device for evaluating the regulatory function of intestinal flora. Due to the design of an elastic adhesive layer, after multiple inoculations or extractions of the flora, the overall thickness increases due to the multiple elastic adhesive layers covering the device. Furthermore, the puncture sites formed by the flora extraction syringe are also obscured by the new elastic adhesive layers. During subsequent inoculations or extractions, it is impossible to ensure that the puncture positions of the flora extraction syringe on the multiple elastic adhesive layers remain consistent. As a result, the flora extraction syringe will re-puncture multiple elastic adhesive layers, which increases the difficulty of puncture. Therefore, the experimenter needs to increase the puncture force, but this can easily lead to the device being punctured due to excessive force, resulting in the failure of the flora culture experiment.

[0035] The detachable top cover 3 designed in this patent application can be placed horizontally into the culture container 2 from top to bottom during use. During the placement process, the top cover 3 needs to be rotated to ensure that the slider 33 on the outer side wall corresponds to the vertical part of the guide groove 22 on the inner side wall of the culture container 2. Then, the top cover 3 is pressed down to make the slider 33 be placed from the upper vertical part of the guide groove 22 from top to bottom. As the top cover 3 is pressed down, the slider 33 moves to the bending part of the guide groove 22. At this time, the bottom part of the extension tube 31 below the top cover 3 will cover the spherical part at the top of the plug 21.

[0036] As the spherical part at the top of the plug 21 is gradually inserted into the opening at the bottom of the extension tube 31, the silicone layer 32 on the inner wall of the bottom of the extension tube 31 will come into contact with and be squeezed around the spherical part at the top of the plug 21. At this time, the squeezed silicone layer 32 will fill the gap between the inner wall of the opening at the bottom of the extension tube 31 and the spherical part at the top of the plug 21, so that the spherical part at the top of the plug 21 can block the opening at the bottom of the extension tube 31, so that the bacterial community inside the culture container 2 will not be affected by external objects during the culture experiment. Then, the top cover 3 can be rotated to make the slider 33 move along the trajectory of the lower half of the guide groove 22, so that the lower half of the guide groove 22 restricts the rise and fall of the slider 33, thereby preventing the top cover 3 from coming off upwards during the covering of the culture container 2.

[0037] Secondly, during the microbial culture experiment, if it is necessary to inoculate, add, or extract microorganisms inside the culture container 2, the top cover 3 can be rotated in the opposite direction and then lifted up, so that the slider 33 moves from the horizontal area of ​​the lower half of the guide groove 22 to the vertical area of ​​the upper half. At this time, since the slider 33 on the outer wall of the top cover 3 is no longer limited by the horizontal area of ​​the lower half of the guide groove 22, the extension tube 31 below can be moved upwards towards the spherical part at the top of the plug 21 when the top cover 3 is lifted up. This allows the squeezed silicone layer 32 to detach from the spherical part at the top of the plug 21, so that a certain gap is created between the bottom end of the extension tube 31 and the plug 21. Then, the experimenter can operate the microbial extraction syringe to insert it through the puncture hole on the top cover 3 and reach the inside of the culture container 2 through the extension tube 31, thereby completing the inoculation, addition, or extraction of microorganisms.

[0038] In a further preferred embodiment, the top end of the extension tube 31 extends a certain distance towards the upper part of the top cover 3, and the extension tube 31 located above the top cover 3 and the extension tube 31 located below the top cover 3 have the same shape and are designed in a symmetrical state. That is, the tube body of the extension tube 31 above the top cover 3 gradually expands outward from bottom to top, so that the external shape of the extension tube 31 located above the top cover 3 forms an inverted frustum shape.

[0039] The top cover 3 is provided with a lever plate 34 on both sides of the extension tube 31, and the lever plate 34 is provided with a slot. The lever plate 34 is fixedly connected to the top cover 3 and the extension tube 31.

[0040] The lever plate 34 and slotted part designed above the top cover 3 are hand-operated by the experimenter to facilitate the rotation and lifting control of the entire top cover 3. The extension tube 31 designed above the top cover 3 can form a support connection between the lever plates 34 on both sides, thereby increasing the stability when the lever plates 34 are connected to the top cover 3.

[0041] Secondly, the extension of the upper extension tube 31 of the top cover 3 and the lower extension tube 31 of the top cover 3 are designed symmetrically, so that when the tip of the bacterial extraction syringe is inserted into the culture container 2, the bacterial extraction syringe can be tilted slightly so that the tip of the syringe passes between the ball part at the top of the plug 21 and the extension tube 31 to extract the bacterial community. The design of the upper extension tube 31 of the top cover 3 gradually expanding outward from bottom to top can provide more room for the bacterial extraction syringe to operate at an angle.

[0042] The above description is merely an embodiment of this utility model and is not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principle of this utility model should be included within the scope of the claims of this utility model.

Claims

1. A well plate structure for culturing intestinal flora, characterized in that, Including culture plate (1); The culture plate (1) has a plurality of placement holes (11) arranged on it, and a culture container (2) is provided in the area of ​​each placement hole (11); The bottom shape of the culture container (2) matches the shape of the placement hole (11) on the culture plate (1), and the bottom part of the culture container (2) can be placed in the placement hole (11) of the culture plate (1). The bottom outer wall of the culture container (2) is provided with threads, and the inner wall of each hole (11) on the culture plate (1) is provided with a threaded groove that matches the threads on the outer wall of the culture container (2). The interior of the culture container (2) is a chamber, and the chamber extends from the top of the culture container (2); The culture container (2) is provided with a removable top cover (3) that can cover the chamber area.

2. The intestinal flora culture plate structure according to claim 1, characterized in that, The top cover (3) has a puncture hole at its center that extends through its top and bottom ends. This puncture hole is used to allow the microbial extraction syringe to enter the chamber of the culture container (2) to perform microbial inoculation or sampling operations. The top cover (3) is provided with an extension tube (31) that matches the size of the puncture hole at the puncture hole. The extension tube (31) is provided with both ends through it, and the bottom end of the extension tube (31) extends a certain distance to the lower part of the top cover (3).

3. The intestinal flora culture plate structure according to claim 2, characterized in that, The tube body of the extension tube (31) located below the top cover (3) gradually expands outward from top to bottom, so that the external shape of the extension tube (31) located below the top cover (3) forms a frustum shape. A silicone layer (32) is fixedly provided on the inner wall of the bottom opening of the extension tube (31).

4. The intestinal flora culture plate structure according to claim 1, characterized in that, A plug (21) is provided at the center of the bottom of the culture container (2), and the bottom end of the plug (21) is fixedly connected to the bottom of the culture container (2).

5. The intestinal flora culture plate structure according to claim 4, characterized in that, The top of the plug (21) is designed as a spherical structure. The shape and size of the sphere match the shape of the bottom opening of the extension tube (31) below the top cover (3). The spherical part at the top of the plug (21) can be inserted into the bottom opening of the extension tube (31) and form a blockage at the bottom of the extension tube (31).

6. The intestinal flora culture plate structure according to claim 2, characterized in that, A guide groove (22) is provided on the inner wall of the top of the culture container (2). The upper half of the guide groove (22) is designed with a vertical trajectory, and the lower half of the guide groove (22) is designed with a horizontal trajectory. The height of the bottom of the vertical part of the upper half of the guide groove (22) matches the height of the top of the extension tube (31) below the top cover (3) fitted onto the ball part at the top of the plug (21). The outer wall of the top cover (3) is fixedly provided with a slider (33) that matches the width of the guide groove (22). When the top cover (3) is inserted into the chamber area of ​​the culture container (2), the slider (33) can be driven to move in the guide groove (22).

7. The intestinal flora culture plate structure according to claim 2, characterized in that, The top end of the extension tube (31) extends a certain distance towards the upper part of the top cover (3), and the extension tube (31) above the top cover (3) and the extension tube (31) below the top cover (3) have the same shape and are designed in a symmetrical state. That is, the tube body of the extension tube (31) above the top cover (3) gradually expands outward from bottom to top, so that the external shape of the extension tube (31) above the top cover (3) forms an inverted frustum shape. The top cover (3) is provided with a lever (34) on both sides of the extension tube (31), and the lever (34) is provided with a slot. The lever (34) is fixedly connected to the top cover (3) and the extension tube (31).

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