A feeding device for synthesizing dNTPs molecular diagnostic raw materials

Abstract

The utility model belongs to dNTPs production technical field, concretely relates to a kind of feeding device of dNTPs molecular diagnostic raw material synthesis.The device includes base, and base top is fixed with reaction box, and reaction box top is equipped with sealing cover, and sealing cover top right side is equipped with feeding mechanism, and reaction box right side is installed with the discharge pipe of first valve.Feeding mechanism contains the feeding hopper of intercommunication sealing cover, and installation rod is inserted in feeding hopper, and there are two rubber sealing pads with spacing on the outside of installation rod, and the side of bottom sealing pad is equipped with inclined plane.The device maintains the pressure required for reaction by the sealing structure of sealing cover and reaction box, realizes the addition of raw material or catalyst using the double-sealing-pad design of feeding mechanism without destroying the internal pressure, solves the problem of pressure change caused by feeding in a sealed and pressurized environment, and meets the use requirements of dNTPs molecular diagnostic raw material synthesis.

Description

Technical Field

[0001] This utility model relates to the field of dNTP production technology, specifically to a feeding device for the synthesis of dNTP molecular diagnostic raw materials. Background Technology

[0002] dNTPs (including dTTPs) are the core raw materials for DNA synthesis and repair, and are widely used in molecular diagnostic technologies such as PCR amplification, DNA sequencing and in vitro mutagenesis. During their synthesis, the reaction often needs to be carried out in a sealed and pressurized environment to ensure efficiency and product purity (≥99%).

[0003] Traditional feeding methods, when adding raw materials or catalysts during a sealed and pressurized reaction, can easily lead to sudden pressure changes in the reaction system, disrupting the stability of the reaction environment and affecting the synthesis efficiency and product quality of dNTPs, thus failing to meet the precision production requirements of molecular diagnostic raw materials. Therefore, a device that can stably feed materials under a sealed and pressurized environment is proposed. Utility Model Content

[0004] The present invention aims to solve the problems mentioned in the background art by providing a feeding device for the synthesis of dNTPs molecular diagnostic raw materials.

[0005] The specific technical solution is as follows:

[0006] A feeding device for the synthesis of dNTPs molecular diagnostic raw materials includes a base, a reaction chamber fixedly installed on the top of the base, a sealing cover provided on the top of the reaction chamber, a feeding mechanism provided on the right side of the top of the sealing cover, a discharge pipe fixedly installed on the right side of the reaction chamber, and a first valve fixedly installed inside the discharge pipe.

[0007] The feeding mechanism includes a feeding hopper connected to the inside of the sealing cover. An installation rod is inserted inside the feeding hopper. Two rubber sealing gaskets are fixedly installed on the outside of the installation rod. One side of the bottom rubber sealing gasket has an inclined surface, and there is a gap between the two rubber sealing gaskets.

[0008] In a preferred embodiment of this utility model, an mounting plate is fixedly installed on the top of the feeding hopper, and an electric push rod is fixedly installed on the top of the mounting plate. The output end of the electric push rod passes through the mounting plate and is fixedly connected to the top of the mounting rod.

[0009] As a preferred embodiment of this utility model, a drive motor is fixedly installed on the top of the sealing cover, and the output end of the drive motor extends into the interior of the reaction chamber and is fixedly installed with a stirring rod.

[0010] As a preferred embodiment of this utility model, an electrical connector is fixedly installed on the top of the sealing cover and on the left side of the drive motor, a heating rod is fixedly installed on the bottom of the electrical connector, the heating rod extends into the interior of the reaction chamber, and a temperature sensor is fixedly installed on the bottom of the electrical connector, and the temperature sensor penetrates the sealing cover and extends to the bottom of the sealing cover.

[0011] As a preferred embodiment of this utility model, a pressure tube is connected to the top of the sealing cover and the front side of the electrical connector, and a second valve is fixedly installed inside the pressure tube.

[0012] As a preferred embodiment of this utility model, connecting blocks are fixedly installed on both sides of the sealing cover and the reaction chamber, and bolts are inserted inside the connecting blocks.

[0013] As a preferred embodiment of this utility model, the top of the reaction chamber is provided with a receiving groove, a rubber sealing ring is provided inside the receiving groove, and a sealing energy ring is fixedly installed at the bottom of the sealing cover. The sealing energy ring penetrates into the receiving groove and contacts the top of the rubber sealing ring.

[0014] This utility model has the following beneficial effects:

[0015] 1. Achieve stable feeding in a sealed environment

[0016] The double rubber sealing gasket design of the feeding mechanism, combined with the precise drive of the electric push rod, can maintain pressure stability in the sealed and pressurized environment of dNTP synthesis by the sealing gasket adhering to the feeding hopper, and can also complete the feeding by utilizing the gap. This solves the problem of sudden pressure change caused by traditional feeding and ensures the continuity of the dNTP synthesis reaction.

[0017] 2. Improve reaction efficiency and product purity

[0018] The mixing action of the stirring rod ensures that dNMP and raw materials such as triphosphates come into full contact, reducing uneven local reactions;

[0019] The coordinated control of the heating rod and temperature sensor ensures that the reaction always takes place at a suitable temperature;

[0020] The pressure regulation function of the pressure tube is adapted to the pressure requirements of dNTP synthesis. The three together improve reaction efficiency and help the product purity reach molecular diagnostic grade standards.

[0021] 3. Ease of operation and adaptability

[0022] The device uses an electric push rod to automatically control the feeding, and the bolted connection makes it easy to disassemble and clean the sealing cover. It can be flexibly adapted to the synthesis requirements of different dNTPs (such as dTTP), improving the controllability and practicality of the production process. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the feeding device for synthesizing dNTPs molecular diagnostic raw materials according to an embodiment of the present invention;

[0024] Figure 2 A schematic diagram of the reaction chamber structure of the feeding device for synthesizing dNTPs molecular diagnostic raw materials provided in this embodiment of the present invention;

[0025] Figure 3 A bottom view of the sealing cap structure of the feeding device for synthesizing dNTPs molecular diagnostic raw materials provided in this embodiment of the utility model;

[0026] Figure 4 A schematic diagram of the drive motor and stirring rod structure of the feeding device for synthesizing dNTPs molecular diagnostic raw materials provided in this embodiment of the utility model;

[0027] Figure 5 This is a schematic diagram of the feeding mechanism of the feeding device for synthesizing dNTPs molecular diagnostic raw materials provided in this embodiment of the utility model.

[0028] In the attached diagram: 1. Base; 2. Reaction chamber; 3. Feeding mechanism; 301. Feeding hopper; 302. Mounting plate; 303. Electric push rod; 304. Mounting rod; 305. Rubber sealing gasket; 306. Inclined surface; 4. Sealing cover; 5. Bolt; 6. Connecting block; 7. Discharge pipe; 8. First valve; 9. Drive motor; 10. Electrical connector; 11. Pressurization pipe; 12. Second valve; 13. Receiving groove; 14. Heating rod; 15. Sealing ring; 16. Stirring rod. Detailed Implementation

[0029] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments.

[0030] The accompanying drawings are for illustrative purposes only and are schematic diagrams, not actual images. They should not be construed as limiting the scope of this patent. To better illustrate the embodiments of this utility model, some components in the drawings may be omitted, enlarged, or reduced, and do not represent the actual dimensions of the product. It is understandable to those skilled in the art that some well-known structures and their descriptions may be omitted in the drawings.

[0031] In the accompanying drawings of this utility model, the same or similar reference numerals correspond to the same or similar components. In the description of this utility model, it should be understood that if terms such as "upper," "lower," "left," "right," "inner," and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, they are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms used to describe positional relationships in the drawings are only for illustrative purposes and should not be construed as limiting this patent. For those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.

[0032] In the description of this utility model, unless otherwise explicitly specified and limited, the term "connection" or similar designation indicating the connection relationship between components should be interpreted broadly. For example, it can refer to a fixed connection, a detachable connection, or an integral part; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0033] Example

[0034] The feeding device for the synthesis of dNTPs molecular diagnostic raw materials provided in this embodiment, such as... Figures 1-5 As shown, the system includes a base 1, a reaction chamber 2 fixedly mounted on the top of the base 1, a sealing cover 4 on the top of the reaction chamber 2, a feeding mechanism 3 on the right side of the top of the sealing cover 4, a discharge pipe 7 fixedly mounted on the right side of the reaction chamber 2, and a first valve 8 fixedly mounted inside the discharge pipe 7. The reaction chamber 2 is used to hold raw materials for subsequent reactions. The sealing cover 4 is used to seal the top of the reaction chamber 2 to meet the pressure requirements during subsequent reactions. The discharge pipe 7 and the first valve 8 are used to discharge the reaction products. The feeding mechanism 3 is used to add raw materials into the interior of the reaction chamber 2. At the same time, when the internal pressure is greater than the external pressure during subsequent reactions, the required catalyst or raw materials are added into the interior of the reaction chamber 2.

[0035] The feeding mechanism 3 includes a feeding hopper 301 connected to the inside of the sealing cover 4. An installation rod 304 is inserted inside the feeding hopper 301. Two rubber sealing gaskets 305 are fixedly installed on the outside of the installation rod 304. One side of the bottom rubber sealing gasket 305 has an inclined surface 306. There is a gap between the two rubber sealing gaskets 305. The feeding hopper 301 is used to add raw materials or catalysts into the reaction chamber 2. The installation rod 304 is used to install the rubber sealing gaskets 305. The rubber sealing gaskets 305 are used to seal the feeding hopper 301 to prevent the high pressure environment inside the reaction chamber 2 from leaking out and causing the internal sub-pressure to decrease, which would affect the reaction. The gap between the two rubber sealing gaskets 305 facilitates the containment of raw materials or catalysts. Thus, the raw materials or catalysts are added into the reaction chamber 2 at the same time as the rubber sealing gaskets 305 slide inside the feeding hopper 301 to achieve a seal.

[0036] An installation plate 302 is fixedly installed on the top of the feeding hopper 301. An electric push rod 303 is fixedly installed on the top of the installation plate 302. The output end of the electric push rod 303 passes through the installation plate 302 and is fixedly connected to the top of the installation rod 304. The electric push rod 303 is used to push the installation rod 304 to drive the rubber sealing gasket 305 to rise, thereby realizing the addition of raw materials or catalysts.

[0037] A drive motor 9 is fixedly installed on the top of the sealing cover 4. The output end of the drive motor 9 extends into the interior of the reaction chamber 2 and a stirring rod 16 is fixedly installed thereon. The drive motor 9 and the stirring rod 16 are used to stir the raw materials inside the reaction chamber 2 to accelerate the reaction.

[0038] An electrical connector 10 is fixedly installed on the top of the sealing cover 4 and to the left of the drive motor 9. A heating rod 14 is fixedly installed at the bottom of the electrical connector 10, extending into the interior of the reaction chamber 2. A temperature sensor is fixedly installed at the bottom of the electrical connector 10, and the temperature sensor passes through the sealing cover 4 and extends to the bottom of the sealing cover 4. The electrical connector 10 enables the connection between the heating rod 14 and the external power cord. The heating rod 14 is used to heat the raw materials inside the reaction chamber 2 to promote the subsequent reaction. The temperature sensor is used to monitor the temperature inside the reaction chamber 2.

[0039] A pressure pipe 11 is connected to the top of the sealing cap 4 and to the front of the electrical connector 10. A second valve 12 is fixedly installed inside the pressure pipe 11. The pressure pipe 11 is designed to inject gas into the interior to increase the internal pressure and thus promote the subsequent reaction.

[0040] Both sides of the sealing cover 4 and the reaction chamber 2 are fixedly installed with connecting blocks 6. Bolts 5 are inserted inside the connecting blocks 6. The bolts 5 and the connecting blocks 6 are used to fix the sealing cover 4 relative to the reaction chamber 2. At the same time, the sealing cover 4 can be removed to facilitate the cleaning of the inside of the reaction chamber 2 by removing the bolts 5.

[0041] The top of the reaction chamber 2 is provided with a receiving groove 13, and a rubber sealing ring is provided inside the receiving groove 13. A sealing energy ring 15 is fixedly installed at the bottom of the sealing cover 4. The sealing energy ring 15 extends through the inside of the receiving groove 13 and contacts the top of the rubber sealing ring. The receiving groove 13, the rubber gasket and the sealing energy ring 15 are provided to increase the airtightness between the reaction chamber 2 and the sealing cover 4, and promote the subsequent internal high pressure environment to provide the necessary environment for the reaction.

[0042] Working principle

[0043] This device achieves stable maintenance of the sealed environment and precise feeding during the synthesis of dNTPs through the coordinated action of multiple components. The specific principle is as follows:

[0044] 1. Sealing and pressurization foundation

[0045] The reaction chamber 2 is used to hold the raw materials for dNTP synthesis, and its top is sealed by the sealing cover 4. The sealing ring 15 at the bottom of the sealing cover 4 is embedded in the receiving groove 13 at the top of the reaction chamber 2 and cooperates with the rubber sealing ring in the groove to ensure the airtightness of the reaction chamber 2 (meeting the sealing requirements for dNTP synthesis).

[0046] The pressurization pipe 11 can introduce inert gas (which does not react with the raw materials) into the reaction chamber 2 through the second valve 12 to adjust the internal pressure to meet the pressurization requirements of dNTPs synthesis.

[0047] 2. Precision feeding mechanism

[0048] The feeding mechanism 3 is the core component, with its feeding hopper 301 connected to the inside of the sealing cover 4. The two rubber sealing gaskets 305 on the outside of the mounting rod 304 can slide inside the feeding hopper 301.

[0049] In the initial state, the rubber sealing gasket 305 is tightly attached to the inner wall of the feeding hopper 301, blocking the connection between the reaction chamber 2 and the outside world and maintaining the internal pressure;

[0050] When feeding is required, the electric push rod 303 drives the mounting rod 304 to move the rubber sealing gasket 305. The raw materials / catalyst are temporarily stored in the gap between the two sealing gaskets. The inclined surface 306 of the bottom sealing gasket guides the material into the reaction chamber 2, realizing feeding without pressure loss in a sealed environment (avoiding affecting the pressure stability of dNTP synthesis).

[0051] 3. Reaction condition control

[0052] The drive motor 9 drives the stirring rod 16 to rotate, so that dNMP, triphosphate and metal ions in the reaction chamber 2 are fully mixed (improving reaction efficiency);

[0053] The heating rod 14 is powered by the electrical connector 10 and, together with the temperature sensor, controls the temperature inside the reaction chamber 2 in real time (to meet the temperature requirements for dNTPs synthesis).

[0054] How to use

[0055] The specific steps for synthesizing dNTPs are as follows:

[0056] 1. Initial raw material addition

[0057] Start the electric push rod 303, drive the mounting rod 304 to raise the rubber sealing gasket 305, so that the feeding hopper 301 is connected to the reaction box 2;

[0058] Initial raw materials such as dNMP and triphosphate are added to the feeding hopper 301, and the raw materials enter the reaction chamber 2 through the feeding hopper 301.

[0059] The electric push rod 303 is activated in reverse to reset the rubber sealing gasket 305 and seal the feeding hopper 301. The connecting block 6 between the sealing cover 4 and the reaction chamber 2 is tightened by bolt 5 to ensure a complete seal.

[0060] 2. Add catalyst / raw material midway

[0061] When the synthesis of dNTPs requires the addition of catalysts or raw materials such as magnesium ions, the material is injected into the feeding hopper 301 and the material is temporarily stored between the two rubber sealing gaskets 305.

[0062] The electric push rod 303 is activated to push the mounting rod 304 down, and the inclined surface 306 of the bottom sealing gasket guides the material to slide into the reaction chamber 2. After completion, the sealing gasket is reset to maintain pressure.

[0063] 3. Adjustment of reaction conditions

[0064] The heating rod 14 is activated by the electrical connector 10, and the temperature inside the reaction chamber 2 is adjusted to the range required for dNTPs synthesis by the temperature sensor.

[0065] Open the second valve 12 and introduce inert gas through the pressurization pipe 11 to adjust the pressure inside the reaction chamber 2 to the set value;

[0066] Start the drive motor 9 to make the stirring rod 16 rotate and stir the material, so as to promote the full reaction.

[0067] 4. Product discharge and equipment cleaning

[0068] After the reaction is complete, open the second valve 12 to release the high pressure in the reaction chamber 2 through the pressurization pipe 11. After the internal and external pressures are balanced, open the first valve 8 of the discharge pipe 7 to discharge the dNTPs product.

[0069] Remove bolt 5 and take off the sealing cover 4. Clean the reaction chamber 2 and stirring rod 16 and other components for future use.

[0070] The above are merely preferred embodiments of the present utility model and are not intended to limit the implementation methods and protection scope of the present utility model. Those skilled in the art should realize that any equivalent substitutions and obvious changes made based on the description and illustrations of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A feeding device for the synthesis of molecular diagnostic raw materials of dNTPs, characterized by, Includes a base (1), a reaction chamber (2) is fixedly installed on the top of the base (1), a sealing cover (4) is provided on the top of the reaction chamber (2), a feeding mechanism (3) is provided on the right side of the top of the sealing cover (4), a discharge pipe (7) is fixedly installed on the right side of the reaction chamber (2), and a first valve (8) is fixedly installed inside the discharge pipe (7). The feeding mechanism (3) includes a feeding hopper (301) connected to the inside of the sealing cover (4). An installation rod (304) is inserted inside the feeding hopper (301). Two rubber sealing gaskets (305) are fixedly installed on the outside of the installation rod (304). A slope (306) is opened on one side of the bottom rubber sealing gasket (305). There is a gap between the two rubber sealing gaskets (305).

2. The feeding device for synthesizing dNTPs molecular diagnostic raw materials according to claim 1, characterized in that, An installation plate (302) is fixedly installed on the top of the feeding hopper (301), and an electric push rod (303) is fixedly installed on the top of the installation plate (302). The output end of the electric push rod (303) passes through the installation plate (302) and is fixedly connected to the top of the installation rod (304).

3. The feeding device for synthesizing dNTPs molecular diagnostic raw materials according to claim 1, characterized in that, A drive motor (9) is fixedly installed on the top of the sealing cover (4), and the output end of the drive motor (9) extends into the interior of the reaction chamber (2) and is fixedly installed with a stirring rod (16).

4. The feeding device for synthesizing dNTPs molecular diagnostic raw materials according to claim 1, characterized in that, An electrical connector (10) is fixedly installed on the top of the sealing cover (4) and on the left side of the drive motor (9). A heating rod (14) is fixedly installed on the bottom of the electrical connector (10). The heating rod (14) extends into the interior of the reaction chamber (2). A temperature sensor is fixedly installed on the bottom of the electrical connector (10), and the temperature sensor passes through the sealing cover (4) and extends to the bottom of the sealing cover (4).

5. The feeding device for synthesizing dNTPs molecular diagnostic raw materials according to claim 4, characterized in that, A pressure pipe (11) is connected to the top of the sealing cap (4) and to the front side of the electrical connector (10), and a second valve (12) is fixedly installed inside the pressure pipe (11).

6. The feeding device for synthesizing dNTPs molecular diagnostic raw materials according to claim 5, characterized in that, Both sides of the sealing cover (4) and the reaction chamber (2) are fixedly installed with connecting blocks (6), and bolts (5) are inserted inside the connecting blocks (6).

7. The feeding device for synthesizing dNTPs molecular diagnostic raw materials according to any one of claims 1-6, characterized in that, The top of the reaction chamber (2) is provided with a receiving groove (13), and a rubber sealing ring is provided inside the receiving groove (13). A sealing energy ring (15) is fixedly installed at the bottom of the sealing cover (4). The sealing energy ring (15) penetrates into the inside of the receiving groove (13) and contacts the top of the rubber sealing ring.

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