Compositions and methods for detecting gastrointestinal pathogens
Specific oligonucleotide sets and multiplex assays allow for efficient and sensitive detection of gastrointestinal pathogens, improving diagnostic accuracy and patient care for bacterial gastroenteritis.
JP2025116130AActive Publication Date: 2025-08-07GEN PROBE INC
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Patent Information
- Application Number
- JP2025089744
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2021-07-27
- Filing Date
- 2025-05-29
- Publication Date
- 2025-08-07
- Estimated Expiration
- 2042-07-26
AI Technical Summary
Technical Problem
There is a need for efficient and sensitive detection of gastrointestinal pathogens such as Salmonella, Shigella, Campylobacter, and Shigatoxigenic Escherichia coli (STEC) in biological specimens to provide diagnostic and prognostic information for patients with bacterial gastroenteritis.
Method used
The use of specific oligonucleotide sets for amplification, including Salmonella-specific, C. jejuni-specific, C. coli-specific, Shigella-specific, and STEC-specific oligomers, along with multiplex assays and fluorescent detection probes, to identify the presence of these pathogens in samples.
Benefits of technology
Enables rapid and accurate detection of multiple gastrointestinal pathogens, enhancing diagnostic capabilities and patient management for bacterial gastroenteritis.
✦ Generated by Eureka AI based on patent content.
Abstract
To provide compositions and methods for detecting gastrointestinal pathogens.SOLUTION: Compositions, methods, kits, and uses are provided for detecting the presence of Salmonella, Shigella, Campylobacter, and Shigatoxigenic Eschelichia coli (STEC) in samples. In some embodiments, the compositions, methods, kits, and uses comprise one or more oligonucleotides or the use of one or more oligonucleotides. In some embodiments, the method is carried out as a multiplex assay with separate primers / probes for C.jejuni and C.coli. Some of the oligonucleotides contain inosine and / or contain 5-methylcytosine substituted nucleotides. Described is the use of cyclodextrin and / or Polysorbate 20 in the amplification reactions.SELECTED DRAWING: None
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