Method for preparing a flow cell

Abstract

In one example of a method for fabricating a flow cell, a functional material is deposited on a resin layer containing recesses separated by gap regions. The resin layer contains an ultraviolet (UV) light-blocking additive. The recesses lie on a first portion of the resin layer having a first thickness, and the gap regions lie on a second portion of the resin layer having a second thickness greater than the first thickness. The functional material readily interacts with the resin layer when exposed to UV light. A predetermined amount of UV light is irradiated through the resin layer, thereby exposing the functional material in the recesses to UV light and causing it to adhere to the resin layer within the recesses. The functional material above the gap regions is prevented from being exposed to UV light by the second portion of the resin.

Claims

1. It is a method, The method involves depositing a functional material on a resin layer that includes recesses separated by gap regions, The aforementioned resin layer contains an ultraviolet (UV) light blocking additive. The recess is located on a first portion of the resin layer having a first thickness, and the gap region is located on a second portion of the resin layer having a second thickness greater than the first thickness. The aforementioned functional material readily interacts with the resin layer when exposed to UV light, and is deposited in such a manner. A method comprising irradiating the resin layer with a first predetermined amount of UV light, thereby exposing the first functional material in the recess to the first predetermined amount of UV light and adhering to the resin layer in the recess, and preventing the first functional material above the gap region from being exposed to the first predetermined amount of UV light by the second resin portion.

2. The method according to claim 1, wherein the functional material is a polymer hydrogel that adheres to the resin layer in the recess when exposed to the predetermined amount of UV light.

3. The method according to claim 2, wherein the polymer hydrogel is pre-grafted with a primer set.

4. The method according to claim 2, further comprising grafting a primer set onto the polymer hydrogel.

5. The method according to claim 1, wherein the functional material is a polymer hydrogel coated on core particles and adheres to the resin layer in the recess when exposed to a predetermined amount of UV light.

6. The method according to claim 5, further comprising, after exposure to the predetermined amount of UV light, attaching a primer set to the polymer hydrogel coated on the core particles.

7. The method according to claim 1, wherein the functional material includes a primer set that adheres to the resin layer in the recess when exposed to the predetermined amount of UV light.

8. The method according to any one of claims 1 to 7, wherein the resin layer contains about 1% by weight to about 50% by weight of the UV light blocking additive based on the total weight of the resin layer.

9. It is a method, Introducing a functional material into a flow cell comprising two patterned substrates, each of which comprises a resin layer having recesses separated by gap regions. The aforementioned resin layer contains an ultraviolet (UV) light blocking additive. Each of the two patterned substrates has a recess on a first portion of the resin layer having a first thickness, and each of the two patterned substrates has a gap region on a second portion of the resin layer having a second thickness greater than the first thickness. The functional material is introduced such that it readily interacts with the resin layers of the two patterned substrates when exposed to UV light. A method comprising simultaneously irradiating a predetermined amount of UV light through the resin layer of each of the two patterned substrates, so that a portion of the functional material in the recess of the first substrate of the two patterned substrates is exposed to the predetermined amount of UV light and adheres to the resin layer in the recess of the first substrate of the two patterned substrates, and another portion of the functional material in the recess of the second substrate of the two patterned substrates is exposed to the predetermined amount of UV light and adheres to the resin layer in the recess of the second substrate of the two patterned substrates, and the functional material in contact with the gap region of each of the two patterned substrates is prevented from being exposed to the predetermined amount of UV light by the second resin portion.

10. The method according to claim 9, wherein the functional material is a polymer hydrogel that adheres to the resin layer in the recess of the first and second substrates of the two patterned substrates when exposed to a predetermined amount of UV light.

11. The method according to claim 10, wherein the polymer hydrogel is pre-grafted with a primer set.

12. The method according to claim 10, further comprising grafting a primer set onto the polymer hydrogel.

13. The method according to claim 9, wherein the functional material is a polymer hydrogel coated on core particles and adheres to the resin layer in the recesses of the first and second substrates of the two patterned substrates when exposed to a predetermined amount of UV light.

14. The method according to claim 13, further comprising, after exposure to the predetermined amount of UV light, attaching a primer set to the polymer hydrogel coated on the core particles.

15. The method according to claim 9, wherein the functional material includes a primer set that adheres to the resin layer in the recesses of the first and second substrates of the two patterned substrates when exposed to a predetermined amount of UV light.

16. The method according to any one of claims 9 to 15, wherein the resin layer of the first and second substrates of the two patterned substrates contains about 1% to about 50% by weight of the UV light blocking additive, based on the total weight of each of the resin layers.

17. It is a method, The method involves depositing a first functional material on a resin layer that includes recesses separated by interstitial regions, The aforementioned resin layer contains an ultraviolet (UV) light blocking additive. The recess is located on a first portion of the resin layer having a first thickness, and the gap region is located on a second portion of the resin layer having a second thickness greater than the first thickness. The first functional material is readily interacting with the resin layer when exposed to UV light, and is deposited in a manner that facilitates interaction with the resin layer. The method involves irradiating the resin layer with a first predetermined amount of UV light, thereby exposing the first functional material in the recess to the first predetermined amount of UV light and causing it to adhere to the resin layer in the recess, while the first functional material above the gap region is prevented from being exposed to the first predetermined amount of UV light by the second resin portion and remains not adhered to the resin layer in the gap region. Removing the first functional material from the gap region, The method involves depositing a second material on the gap region and on the first functional material within the recess, wherein the second material is deposited in such a way that it readily interacts with the resin layer when exposed to UV light and does not readily interact with the first functional material when exposed to UV light. A method comprising irradiating the second material directly with a second predetermined amount of UV light, thereby exposing the second material to the second predetermined UV light, adhering to the resin layer in the gap region, and remaining not adhering to the first functional material.

18. The method according to claim 17, wherein the second material is a passivation material.

19. The method according to claim 17, wherein the second material is a functionalized material selected from the group consisting of quenchers, antioxidants, and combinations thereof.

20. The method according to any one of claims 17 to 19, wherein the first functional material is a polymer hydrogel that adheres to the resin layer in the recess when exposed to a first predetermined amount of UV light.

21. The method according to claim 20, wherein the polymer hydrogel is pre-grafted with a primer set.

22. The method according to claim 20, further comprising grafting a primer set onto the polymer hydrogel.

23. The method according to any one of claims 17 to 19, wherein the first functional material is a polymer hydrogel coated on core particles and adheres to the resin layer in the recess when exposed to a first predetermined amount of UV light.

24. The method according to any one of claims 17 to 19, wherein the first functional material includes a primer set that adheres to the resin layer in the recess when exposed to a first predetermined amount of UV light.

25. The method according to any one of claims 17 to 24, wherein the resin layer contains about 1% by weight to about 50% by weight of the UV light blocking additive based on the total weight of the resin layer.

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