Methods for delivering cargo to cells

Photoresponsive organic particles effectively deliver cargo into cells by forming vapor bubbles, addressing safety and efficiency issues of inorganic methods, achieving high yield and low cytotoxicity in challenging cell types.

JP7877346B2Active Publication Date: 2026-06-22TRINCE BV
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Patent Information

Authority / Receiving Office
JP Β· JP
Patent Type
Patents
Current Assignee / Owner
TRINCE BV
Filing Date
2022-03-11
Publication Date
2026-06-22

AI Technical Summary

Technical Problem

Existing methods for delivering macromolecules into cells, such as electroporation and nanoparticle-sensitized photoporation, face challenges including high cytotoxicity, genotoxicity, and limited delivery efficiency, particularly in primary cells, and the use of inorganic nanoparticles raises safety concerns.

Method used

A method using photoresponsive organic particles, such as polymer-, protein-, or lipid-based particles, is employed to deliver cargo like mRNA into cells by forming vapor bubbles upon irradiation with electromagnetic radiation, ensuring high delivery efficiency and biocompatibility.

Benefits of technology

The method achieves low cytotoxicity, high cell viability, and efficient cargo delivery, particularly in difficult-to-transfect cells like human T cells, with improved yield and safety compared to conventional methods.

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Abstract

The present invention relates to an in vitro or ex vivo method for delivering a cargo into a cell, comprising the steps of contacting a cell with one or more light-responsive organic particles and a cargo, wherein the cargo is not bound to the one or more light-responsive organic particles, and the organic particles are selected from the group consisting of polymer-based particles, protein-based particles, lipid-based particles, and combinations thereof, thereby obtaining a mixture of cells, cargo, and one or more light-responsive organic particles, and irradiating the mixture of cells, cargo, and one or more light-responsive organic particles with electromagnetic radiation, thereby causing permeabilization of the barrier of the cell and delivering the cargo into the cell.The present invention further relates to one or more light-responsive organic particles as defined herein, and the cargo for use in an in vivo method of delivering the cargo to a cell of a subject.
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