Aquatic animal feed mixed with microorganisms rich in polyhydroxyalkanoates.

Abstract

OCR07AP The aquaculture feed mixed with polyhydroxyalkanoates-rich microorganisms consists of two steps: (a) The production of polyhydroxyalkanoate-rich microorganisms is achieved by culturing Enterobacters p. in a medium. A liquid containing a reducing sugar concentration of 120 grams per liter was used to cultivate microorganisms at a temperature of 37 degrees Celsius. Shaking at 150 revolutions per minute for 72 hours, microorganisms were harvested by centrifugation at that speed. 10,000 revolutions per minute for 15 minutes will yield Enterobacter microbial cells rich in polyhydroxyalkalines. (a) Production of aquaculture feed mixed with the following ingredients: fish meal 42% By weight, soybean meal 25%, by weight, fine bran 4%, and corn 26%. 2% fish oil by weight and Enterobacter microbial cells enriched with polyhydroxyalka. 0.5% by weight of Noate is formed into pellets and air-dried for one day to obtain aquatic animal feed mixed with microorganisms. Rich in polyhydroxyalkanoates, suitable for use in aquaculture.

Claims

OCR09WP31 / 01 / 25691. Aquatic animal feed mixed with polyhydroxyalkanoate-rich microorganisms involves two steps: a. The production of polyhydroxyalkanoate-rich microorganisms is achieved by culturing Enterobacters p. in a liquid medium with a reducing sugar concentration of 120 g / L at 37°C, shaking at 150 rpm for 72 hours. The microorganisms are harvested by centrifugation at 10,000 rpm for 15 minutes, yielding Enterobacter p. cells. a. Production of aquaculture feed: A mixture of various ingredients is prepared as follows: fish meal 42% by weight, soybean meal 25% by weight, fine rice bran 4% by weight, corn 26% by weight, fish oil 2% by weight, and Enterobacter microbial cells rich in polyhydroxyalkanoates 0.5% by weight. The mixture is formed into pellets and air-dried for one day to obtain aquaculture feed mixed with microorganisms rich in polyhydroxyalkanoates for feeding aquatic animals.