Method for preparing corona virus-like particles and vaccine comprising the same

Abstract

The present invention provides a method for preparing coronavirus-like particles and a vaccine comprising the same. The method comprises: constructing a recombinant baculovirus carrying a coronavirus spike protein gene; transducing mosquito cells with the recombinant baculovirus; and culturing transduced cells in serum-free medium to produce coronavirus-like particles, wherein the resulting coronavirus-like particles contain coronavirus spike protein. Furthermore, the transduced cells are preferably C6 / 36 cells, and the coronavirus spike protein gene may be from SARS-CoV, MERS-CoV, or SARS-CoV-2. Additionally, the present invention provides a vaccine containing the coronavirus-like particles prepared by the aforementioned method, which can be administered via intranasal or intramuscular routes.