Compositions of extracellular vesicles and method using the same

Abstract

The present disclosure provides a composition comprising primed extracellular vesicles and a biologically or pharmaceutically acceptable carrier, wherein the primed extracellular vesicles comprise an upregulated miRNA, and a fold change of the upregulated miRNA of the primed extracellular vesicles compared to miRNA of naturally occurring extracellular vesicles is greater than 1. The present disclosure further provides a method for preventing, treating, or ameliorating a bone defect or a dental defect comprising administering the composition of the present disclosure to a subject in need thereof. The present disclosure also provides a method for producing the composition. The method comprises cultivating a stem cell in a culture medium containing a Polygonum multiflorum Thunb extract and at least one selected from the group consisting of baicalin and bone morphogenetic protein 6 to obtain a cell culture.