Method for preparing repair composition and use thereof

Abstract

The present invention relates to a repair composition, a facial mask essence and a preparation method therefor. The repair composition comprises the following components in percentage by weight: 10.0%-30.0% of an animal umbilical cord extract, 20.0%-50.0% of β-1.3 glucan and 20.0%-70.0% of a Portulacae herba extract. The repair composition of the present invention is used in cosmetics, and has the effects of significantly reducing wrinkle formation and delaying skin aging, thereby further achieving the effect of maintaining beauty and youth. The repair composition does not cause users to have an allergic reaction and is safe to use.

Description

Preparation method and application of repair composition Technical Field

[0001] The present invention relates to the field of cosmetics, in particular to an application of a repairing composition in a facial mask essence and a preparation method thereof. Background Art

[0002] During adolescence, the skin of a person is fair, delicate, rosy, and elastic. However, with age, the skin begins to age naturally. Furthermore, factors such as mental stress, nutrition, endocrine function, medications, and ultraviolet radiation accelerate skin aging, manifesting as a sallow, rough complexion, loss of elasticity, skin atrophy, wrinkles, and hyperpigmentation. Skin aging is essentially a decline in the proliferation and metabolic capacity of skin cells. In recent years, the use of animal umbilical cord extract in cosmetics has gained increasing attention. Stem cells are early, undifferentiated cells with the ability to replicate and differentiate into various functional cells. During the process of development and adaptation, the human body retains a portion of these undifferentiated, primitive cells, also known as stem cells, to compensate for the loss of the ability to divide again due to advanced differentiation and the eventual aging and death of normal cells. In their article "Technological Innovation in the Skincare Market," Wang Tianzhuang et al. note the promising potential of stem cell ingredients in skin protection, hydration, and repair. Among the many types of stem cells, adult stem cells, particularly mesenchymal stem cells and skin stem cells, are most relevant to beauty, skincare, and health care. Technical issues

[0003] The purpose of the present invention is to provide an application of a repair composition in a facial mask essence and a preparation method thereof, so as to fill the gap in the prior art. Technical Solutions

[0004] A repair composition comprises the following components by weight: 10.0-30.0% of animal umbilical cord extract, 20.0-50.0% of beta-1.3 glucan, and 20.0-70.0% of purslane extract.

[0005] The preparation method of the animal umbilical cord extract is to culture porcine umbilical cord mesenchymal stem cells in a constant temperature and humidity incubator at 35-40°C and 3%-7% CO2 for 2-4 weeks, collect the cell culture supernatant, and then centrifuge and mix the supernatant, filter, and obtain the filtrate.

[0006] The preparation method of the purslane extract comprises the following steps:

[0007] (1) Clean the purslane, dry it, and crush it for later use;

[0008] (2) Mix purslane and corn alcohol in a weight ratio of 1:3~5, stir thoroughly, soak for 40~50 hours, stir thoroughly, and then filter with gauze to obtain the filtrate;

[0009] (3) The filtrate from step (2) was distilled in a vacuum pot at a temperature of 55-60°C and a pressure of -0.10-0.05 MPa. When 5%-15% of the filtrate in the pot remained, heating was stopped, the filtrate was discharged, 1% of pure water was added, the mixture was stirred evenly, allowed to stand, and then filtered with gauze to obtain the filtrate.

[0010] (4) Wash the ion exchange resin, then rinse it with HCL solution, and then rinse it with NaOH solution. The amount of acid and alkali used each time is about 1 to 3 times the volume of the ion exchange resin, and it is passed dynamically downstream. Between changing the acid and alkali, it is necessary to use a large amount of pure water to wash the acid and alkali to make it deform. Finally, soak it in ethanol for another 1 to 3 hours. Soak the pretreated resin in deionized water for 10 to 15 hours, and then rinse it with deionized water until there is no odor, and then set aside;

[0011] (5) Install the column. Fix the resin column vertically, plug a ball of absorbent cotton into the bottom of the column, and then fill the column with 1,3-butanediol (40% to 60% of the column volume). Then fill the resin from step (4) into the column. The height of the resin should be 2 / 3 to 3 / 4 of the column. After the column is installed, plug a ball of absorbent cotton into the top and set aside.

[0012] (6) Decolorization: drain the filtrate from step (3) into the column described in step (5), and filter and decolorize at a flow rate of 1 to 3 mL per minute to obtain a light yellow purslane extract;

[0013] (7) The purslane extract of step (6), 1.3-butanediol, and alcohol are mixed in a weight ratio of 1:3-5:0.5-1.5, and then placed in a vacuum pot for distillation at a temperature of 55-60°C and a vacuum degree of -0.10-0.08MPa to obtain the product.

[0014] The preparation method of the purslane extract further comprises the following steps: (1) cleaning the purslane, drying it, and crushing it for later use;

[0015] (2) Mix purslane and corn alcohol in a weight ratio of 1:4, stir thoroughly, soak for 48 hours, stir thoroughly, and then filter with gauze to obtain the filtrate;

[0016] (3) The filtrate from step (2) was distilled in a vacuum pot at a temperature of 56°C and a pressure of -0.08 MPa. When 10% of the filtrate remained in the pot, heating was stopped, the filtrate was discharged, 1% of pure water was added, the mixture was stirred evenly, allowed to stand, and then filtered with gauze to obtain the filtrate.

[0017] (4) Wash the ion exchange resin, then rinse it with HCL solution, and then rinse it with NaOH solution. The amount of acid and alkali used each time is about 2 times the volume of the ion exchange resin, and it is passed dynamically downstream. Between changing the acid and alkali, it is necessary to use a large amount of pure water to wash the acid and alkali to make it deform. Finally, soak it in ethanol for another 2 hours. Soak the pretreated resin in deionized water for 12 hours, and then rinse it with deionized water until there is no odor, and then set aside;

[0018] (5) Install the column. Fix the resin column vertically, plug a ball of absorbent cotton into the bottom of the column, and then fill the column with 1,3-butanediol (50% of the column volume). Then fill the resin from step 4) into the column. The height of the resin should be 2 / 3 of the column. After the column is installed, plug a ball of absorbent cotton into the top and set aside.

[0019] (6) Decolorization: drain the filtrate from step (3) into the column described in step (5), filter and decolorize at a flow rate of 2 mL per minute to obtain a light yellow purslane extract;

[0020] (7) The purslane extract of step (6), 1.3-butanediol, and alcohol were mixed in a weight ratio of 1:4:1, and then placed in a vacuum pot for distillation at a temperature of 56°C and a vacuum degree of -0.09 MPa to obtain the product.

[0021] The preparation method of the repair composition comprises the following steps: uniformly mixing animal umbilical cord extract, beta-1.3 glucan and purslane extract to obtain the repair composition.

[0022] The facial mask essence prepared from the repair composition comprises the following components, by weight: 1.0% to 10.0% of the repair composition, 87.5% to 98.3% of an aqueous phase component, and 0.2% to 0.5% of triethanolamine. The aqueous phase of the facial mask essence comprises the following components, by weight: 0.05% to 0.2% of disodium EDTA, 0.05% to 0.5% of allantoin, 1.0% to 6.0% of glycerol, 1.0% to 6.0% of 1,3-butylene glycol, 0.2% to 0.5% of carbomer, and 86.8% to 97.7% of water. Beneficial effects

[0023] The present invention has the following beneficial effects: The animal umbilical cord extract in the repair composition is rich in multiple cell production factors: EGF (epidermal growth factor), b-FGF (fibroblast growth factor), KGF (keratinocyte growth factor), and SCF (stem cell growth factor). These four cell growth factors, when combined, activate multiple signaling proteins within skin cells, promoting skin cell proliferation and repairing damaged skin. β-1.3 glucan effectively helps renew and repair stressed skin, effectively reducing wrinkles, improving skin comfort and moisturizing properties, and promoting scar healing and regeneration. Portulaca oleracea extract has anti-allergic, anti-inflammatory, and analgesic properties. It also has comprehensive properties such as preventing dry and aging skin in winter, increasing skin comfort, and scavenging free radicals, improving skin comfort. When used in cosmetics, the composition significantly reduces wrinkle formation, delays skin aging, and achieves beautifying and nourishing effects, while preventing allergic reactions and ensuring safety. Furthermore, the animal umbilical cord extract prepared by the present invention is derived from human mesenchymal umbilical cord extract and is rich in multiple cell biological factors, exhibiting high bioactivity and concentration, resulting in significant skin effects. The purslane extract prepared by the present invention is also rich in flavonoids, polysaccharides and various unsaturated fatty acids. The use of unique ion exchange resin can protect flavonoids, polysaccharides and various unsaturated fatty acids to the greatest extent, and the anti-allergic and skin care effects are more significant. Best Mode for Carrying Out the Invention

[0024] The technical solutions and advantages of the present invention are further described in detail below with reference to specific embodiments.

[0025] Example 1

[0026] The repair composition of this embodiment includes the following components by weight percentage: 30.0% umbilical cord extract, 30.0% β-1.3 glucan, and 40.0% purslane extract. The β-1.3 glucan was purchased from Sichuan Hetai Xinguang Biotechnology Co., Ltd. under the trade name of Cellex. The preparation method of the repair composition is as follows. The umbilical cord extract is obtained by culturing pig umbilical cord mesenchymal stem cells in a constant temperature and humidity incubator at 37°C and 5% CO2 for 2 to 4 weeks, collecting the cell culture supernatant, and then centrifuging the supernatant in a centrifuge at 2000 rpm for 10 minutes, and then filtering with a 0.22um filter membrane. The filtrate is taken to obtain an umbilical cord extract rich in stem cell growth factor system.

[0027] The preparation of purslane extract comprises the following steps:

[0028] (1) Wash the dried purslane three times until it is clean, then put it into a blast drying oven to dry, and use a Chinese medicine grinder to grind it for later use;

[0029] (2) Mix the crushed purslane and 95% corn alcohol in a rubber bucket at a weight ratio of 1:4, stir thoroughly, and soak for 48 hours, stirring thoroughly 3-5 times in the middle, and then filter with 400 mesh gauze to obtain the filtrate;

[0030] (3) The filtrate from step 2 was distilled in a vacuum pot at 56°C and a pressure of -0.08 mPa. When about 10% of the filtrate remained in the pot, heating was stopped, the filtrate was released, 1% of pure water was added, stirred evenly, and allowed to stand for 24 hours. The filtrate was then filtered through 400-mesh gauze to obtain the filtrate.

[0031] (4) Wash the ion exchange resin purchased on the market thoroughly with deionized water until the water is clear, then rinse it 2-3 times with 4% HCL solution, and then rinse it 2-3 times with 4% NaOH solution. The amount of acid and alkali used each time is about twice the volume of the ion exchange resin, and it is passed through dynamically downstream. Between acid and alkali changes, it is necessary to use a large amount of pure water to wash the acid and alkali to deform it, and finally soak it in 95% ethanol for another 2 hours. Soak the pretreated resin in deionized water for 12 hours, and then rinse it with deionized water until there is no odor, and set aside;

[0032] (5) Install the column. Fix the resin column vertically, insert a ball of absorbent cotton into the bottom of the column, then fill the column with 50% of the column volume of 1,3-butanediol, and then fill the column with resin. The height of the resin is about 2 / 3 of the column. After the column is installed, insert a ball of absorbent cotton into the top and set aside.

[0033] (6) Decolorization: Drain the filtrate from step 3 into the column slowly to avoid impacting the resin under the cotton ball, and then filter and decolorize at a flow rate of 2 ml per minute. During the decolorization process, the filtrate filtered out previously is collected again, and the filtrate in the column must be kept submerged above the resin. When the filtrate is almost submerged above the top cotton ball, pour the previous filtrate back into the column and filter again. When the filtrate surface is almost submerged above the cotton ball, the first filtration and decolorization is completed. A light yellow purslane extract is obtained;

[0034] (7) The purslane extract from step 6, 1.3-butylene glycol, and 95% alcohol are mixed in a weight ratio of 1:4:1, and then distilled in a vacuum pot at a temperature of 56°C and a vacuum degree of -0.09 mPa to distill out the alcohol. When almost no alcohol flows out of the distillation pot, the solution in the pot is taken out, which is the purslane extract.

[0035] The umbilical cord extract, β-1.3 glucan and purslane extract are mixed evenly to obtain the product.

[0036] The facial mask essence containing the umbilical cord extract composition comprises the following components by weight percentage: 10.0% of a repair composition, 87.5% of an aqueous phase component, 0.5% of triethanolamine, and 2.0% of an auxiliary component.

[0037] The aqueous phase component comprises the following components by weight percentage: 0.05% disodium edetate, 0.5% allantoin, 6.0% glycerol, 6.0% 1,3-butylene glycol, 0.5% carbomer, and 86.95% water. The auxiliary components are flavors and preservatives.

[0038] The facial mask essence containing the animal umbilical cord extract composition described in this embodiment is prepared by the following steps:

[0039] (1) Add phase B to the emulsifier, heat to 75°C, keep warm to 85°C, homogenize for 3-5 minutes, and stir until completely dissolved;

[0040] (2) When the temperature drops to 50°C, add Phase C into the emulsifier and stir until uniform;

[0041] (3) When the temperature drops to 35°C, add phase A and phase D into the emulsifying pot and stir until uniform to obtain this product.

[0042] The test results of the performance indicators of the repair composition are as follows.

[0043] Appearance: Colorless, transparent, viscous liquid: Qualified. Determination method: Use visual inspection.

[0044] pH value: 5.0-7.0: Pass. Determination method: Dilute the prepared facial mask essence with water in a ratio of 1:10, then test with a pH meter at room temperature. Once the value stabilizes, the reading is the pH value of the material.

[0045] Viscosity: 10,000-25,000 mPa.s: Pass. Test method: Place 200g of mask essence in a tall beaker and test with a viscometer.

[0046] Heat Stability Test (48°C x 48h): Passed. Test Method: Place approximately 30g of mask essence in a transparent bottle in a constant temperature oven at 48°C for 48 hours. Take out the bottle every 24 hours and compare it to the bottle at room temperature for consistency, color, and other indicators. If all indicators are consistent with those at room temperature, the mask passes the heat resistance test.

[0047] Cold Stability Test (-15°C x 48h): Passed. Test Method: Place approximately 30g of mask essence in a transparent bottle and place in a -15°C refrigerator for 48 hours. Take the bottle out every 24 hours, return it to room temperature, and compare it with a sample kept at room temperature. Check consistency, color, and other indicators for consistency. If all indicators are consistent with those at room temperature, the mask passes the cold resistance test. Industrial Applicability

[0048] The repair composition of the present invention is used in cosmetics, has the effects of significantly reducing wrinkle formation, delaying skin aging, and thus achieving beauty and skin care, and does not cause allergic reactions in users, and is safe to use.

Claims

1. A repair composition, comprising the following components by weight percentage: 10.0-30.0% of animal umbilical cord extract, 20.0-50.0% of β-1.3 glucan, and 20.0-70.0% of purslane extract.

2. The repair composition according to claim 1, characterized in that Calculated by weight percentage, the invention comprises the following components: 20.0-30.0% of animal umbilical cord extract, 30.0-40.0% of beta-1.3 glucan and 40.0-50.0% of purslane extract.

3. The repair composition according to claim 2, characterized in that Calculated by weight percentage, the invention comprises the following components: 30.0% of animal umbilical cord extract, 30.0% of beta-1.3 glucan and 40.0% of purslane extract.

4. The repair composition according to any one of claims 1 to 3, characterized in that The preparation method of the animal umbilical cord extract is to culture pig umbilical cord mesenchymal stem cells in a constant temperature and humidity incubator at 35-40° C. and 3%-7% CO2 for 2-4 weeks, collect the cell culture supernatant, and then centrifuge and mix the supernatant, filter, and obtain the filtrate.

5. The repair composition according to any one of claims 1 to 3, characterized in that The preparation method of the purslane extract comprises the following steps: (1) Wash the purslane, dry it, and crush it for later use; (2) Mix purslane and corn alcohol in a weight ratio of 1:3-5, stir thoroughly, soak for 40-50 hours, stir thoroughly, and then filter with gauze to obtain the filtrate; (3) The filtrate of step (2) is distilled in a vacuum pot at a temperature of 55 to 60°C and a pressure of -0.10 to -0.05 MPa. When 5% to 15% of the filtrate in the pot is left, heating is stopped, the filtrate is discharged, 1% of pure water is added, stirred evenly, allowed to stand, and then filtered with gauze to obtain the filtrate; (4) Wash the ion exchange resin, then rinse it with HCL solution, and then rinse it with NaOH solution. The amount of acid and alkali used each time is about 1 to 3 times the volume of the ion exchange resin, and it is passed dynamically downstream. Between changing the acid and alkali, a large amount of pure water is needed to wash the acid and alkali to make it deform. Finally, soak it in ethanol for another 1 to 3 hours. Soak the pretreated resin in deionized water for 10 to 15 hours, and then rinse it with deionized water until there is no odor, and set it aside; (5) Column loading: fix the resin column vertically, insert a ball of absorbent cotton into the bottom of the column, and then fill the column with 1,3-butanediol (40% to 60% of the column volume). Then, pour the resin from step (4) into the column. The height of the resin should be 2 / 3 to 3 / 4 of the column. After the column is loaded, insert a ball of absorbent cotton into the top and set aside. (6) Decolorization, draining the filtrate from step (3) into the column described in step (5), filtering and decolorizing at a flow rate of 1 to 3 mL per minute to obtain a light yellow purslane extract; (7) The purslane extract of step (6), 1.3-butanediol and alcohol are mixed in a weight ratio of 1:3-5:0.5-1.5, and then distilled in a vacuum pot at a temperature of 55-60°C and a vacuum degree of -0.10-0.08MPa to obtain the product.

6. The repair composition according to claim 5, characterized in that The preparation method of the purslane extract comprises the following steps: (1) Wash the purslane, dry it, and crush it for later use; (2) Mix purslane and corn alcohol in a weight ratio of 1:4, stir well, soak for 48 hours, stir well, and then filter with gauze to collect the filtrate; (3) The filtrate from step (2) was distilled in a vacuum pot at a temperature of 56°C and a pressure of -0.08 MPa. When 10% of the filtrate remained in the pot, heating was stopped, the filtrate was discharged, 1% of pure water was added, stirred evenly, allowed to stand, and then filtered with gauze to obtain the filtrate; (4) Wash the ion exchange resin, then rinse it with HCL solution, and then rinse it with NaOH solution. The amount of acid and alkali used each time is about twice the volume of the ion exchange resin, and it is passed dynamically downstream. Between changing the acid and alkali, a large amount of pure water is needed to wash the acid and alkali to make it deform. Finally, soak it in ethanol for another 2 hours. Soak the pretreated resin in deionized water for 12 hours, and then rinse it with deionized water until there is no odor, and set it aside; (5) Install the column. Fix the resin column vertically, insert a ball of absorbent cotton into the bottom of the column, and then fill the column with 1,3-butanediol (50% of the column volume). Then, fill the resin from step 4) into the column. The height of the resin should be 2 / 3 of the column. After installing the column, insert a ball of absorbent cotton into the top and set aside. (6) Decolorization: drain the filtrate from step (3) into the column described in step (5), filter and decolorize at a flow rate of 2 mL per minute to obtain a light yellow purslane extract; (7) The purslane extract of step (6), 1.3-butylene glycol and alcohol are mixed in a weight ratio of 1:4:1, and then distilled in a vacuum pot at a temperature of 56°C and a vacuum degree of -0.09 MPa to obtain the product.

7. The method for preparing the repair composition according to any one of claims 1 to 6, characterized in that: The method comprises the following steps: uniformly mixing the animal umbilical cord extract according to claim 4, β-1.3 glucan and the purslane extract according to claim 5 to obtain the product.

8. Use of the repair composition according to any one of claims 1 to 6 in the preparation of a cosmetic facial mask.

9. A facial mask essence of a repairing composition, characterized in that: Calculated by weight percentage, the invention comprises the following components: 1.0% to 10.0% of the repair composition according to claims 1 to 6, 87.5% to 98.3% of the aqueous phase component, and 0.2% to 0.5% of triethanolamine. The facial mask essence according to claim 9, characterized in that The water phase component comprises the following components by weight percentage: 0.05% to 0.2% disodium edetate, 0.05% to 0.5% allantoin, 1.0% to 6.0% glycerol, 1.0% to 6.0% 1,3-butylene glycol, 0.2% to 0.5% carbomer, and 86.8% to 97.7% water.