Albino mutant strain white brain fungus of auricularia polytricha and artificial cultivation method therefor

Abstract

The present application discloses an albino mutant strain white brain fungus of Auricularia polytricha and an artificial cultivation method therefor. The albino mutant strain white brain fungus of Auricularia polytricha is Auricularia cornea HZSW-Y7, and is deposited in the China General Microbiological Culture Collection Center on 12 June 2023, with the deposit number CGMCC NO. 40663. The present application provides a new albino mutant strain HZSW-Y7 of Auricularia polytricha, and achieves artificial cultivation of the edible fungus, enriching its market variety supply, and achieving high economic benefits and social benefits.

Description

A white variant strain of Auricularia auricularia auricularia and its artificial cultivation method Technical Field

[0001] The embodiments of the present invention relate to the technical field of edible fungus cultivation, and in particular to a white variant strain of Auricularia auricularia auricularia and an artificial cultivation method thereof. Background Art

[0002] At present, wood ear cultivation is mainly based on sawdust, which causes great damage to forest resources. With the protection of forest resources in my country, the resources of wood ear cultivation substrate are limited, so there is an urgent need to solve the problem of alternative raw materials for wood ear cultivation.

[0003] The fruiting bodies of Auricularia auricula cultivation usually have 2-3 waves, and the picking process of the fruiting bodies in each wave requires a lot of labor, which increases the cost of Auricularia auricula cultivation.

[0004] Summary of the Invention

[0005] To this end, an embodiment of the present invention provides a white variant strain of Auricularia auricularia auricularia and an artificial cultivation method thereof.

[0006] In order to achieve the above objectives, the embodiments of the present invention provide the following technical solutions:

[0007] According to the first aspect of an embodiment of the present invention, the present invention provides a white variant strain of Auricularia cornea, wherein the white variant strain of Auricularia cornea is Auricularia cornea HZSW-Y7, which was deposited in the China General Microorganism Culture Collection Center on June 12, 2023, with a deposit number of CGMCC NO.40663.

[0008] According to a second aspect of the embodiments of the present invention, the present invention provides an artificial cultivation method of the white variant strain of Auricularia auricularia auriculariae, comprising the following steps:

[0009] (1) inoculating the wild Auricularia auricularia fruiting body into a PDA culture medium for a first culture, and then transferring the inoculated fruiting body into a stock culture medium for a second culture to obtain a mother culture;

[0010] (2) inoculating the original strain into a liquid culture medium for cultivation to obtain a shake flask liquid strain;

[0011] (3) inoculating the shake flask liquid strain into a fermentation tank filled with fermentation medium for culturing to obtain a fermentation liquid strain;

[0012] (4) The fermented liquid bacteria are inoculated into the fungus bag containing the cultivation material, and then the fungus bag is cultured, opened, placed on a shelf or hung in a bag, and the germination and earing management are carried out.

[0013] Furthermore, in step (1), the conditions for the first culture are: temperature 25°C, time 15 days; the formula of the original culture medium is: 200g potatoes, 20g glucose, 18g agar powder, 1000ml water; the conditions for the second culture are: temperature 26°C, time 14-16 days.

[0014] Furthermore, in step (2), the formula of the liquid culture medium is: 14g glucose, 4g soy flour, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate, and 1000ml water; the culture conditions are: temperature 24-26°C, rotation speed 160 rpm, and time 7 days.

[0015] Furthermore, in step (3), the formula of the fermentation medium is: 0.6% brown sugar, 0.9% glucose, 0.45% soy flour, 0.07% peptone, 0.1% potassium, 0.06% magnesium, 0.02% defoaming agent, and the balance is water;

[0016] The culture conditions are as follows: adjusting the air intake, maintaining the tank pressure at 0.02-0.03 MPa, controlling the ventilation speed at 5-15 m / s, the temperature at 24-26° C., and the culture time at 6-7 days.

[0017] Furthermore, in step (4), the preparation method of the planting material is as follows: weigh the following raw materials in percentage by weight: 15% poplar sawdust, 75% corn cobs, 6% wheat bran, 2% soybean powder, 1% lime, and 1% gypsum; add each raw material into a stirring pot and dry mix for 7 to 10 minutes, add appropriate amount of water and wet mix for 20 to 25 minutes, and bag the raw material when the pH value reaches 8 to 9 and the moisture content reaches 57% to 59%.

[0018] The present invention conducts extensive research on the cultivation material of the brain white fungus HZSW-Y7. Compared with other wood-decaying fungus cultivation formulas, such as 78% sawdust, 17% wheat bran, 1% soybean meal, 2% corn flour, 1% lime, and 1% gypsum, the cultivation material using corn cobs as the main raw materials is more conducive to rapid mushroom fruiting, suitable for factory production, and improves turnover rate. In addition, the price of corn cobs is lower than that of sawdust, which is conducive to reducing production costs.

[0019] Furthermore, the temperature program for culturing the bacterial bag is: 26-28°C for 1-7 days, 23-24°C for 8-20 days, 24-25°C for 20-35 days, carbon dioxide concentration of 800-1500 ppm, and culturing in dark conditions.

[0020] Furthermore, the size of the mushroom bag is 160mm*370mm, and the opening is a circular opening with a diameter of 1.5 centimeters. 20 microholes are pierced within the circular range to form the circular opening. There are 16 openings on each mushroom bag. After the opening, the mushroom bag is cultured for 3 to 5 days to allow the mycelium to recover.

[0021] Furthermore, the germination management is carried out under dark conditions, including: increasing the indoor humidity between 70% and 80%, maintaining the temperature at 20-25° C., and the carbon dioxide concentration at 800-1300 ppm, and primordia appear after 7-10 days.

[0022] Furthermore, the ear management is carried out in dark conditions, including:

[0023] Management from primordium to young mushroom stage: After the primordium appears, maintain the temperature at 20-25°C, the carbon dioxide concentration at 1000-1500ppm, and the humidity at 80%-90%. After 5-7 days, the primordium will grow and form young mushrooms.

[0024] Management from young mushrooms to mature stage: After the young mushrooms are formed, maintain the temperature at 20-25℃, the carbon dioxide concentration at 1200-2000ppm, and the humidity at 80%-95%. The mushrooms will mature in 10-15 days and can be harvested when they reach a diameter of 5-8cm.

[0025] Conventional Auricularia auricularia strains are generally grown at a temperature of 20-25°C, a carbon dioxide concentration of less than 800ppm, and a light intensity of 300-1000LX from the primordium generation stage to the young mushroom (ear) stage. From the young mushroom (ear) stage to the mature stage, they are grown at a temperature of 20-25°C, a carbon dioxide concentration of less than 800ppm, a light intensity of 300-1000LX, and a humidity of 85%-99%. The present inventors have discovered that if ear-growing management is performed under the above conventional conditions, deformed mushrooms will appear, and horn-like tissue will develop on the surface of the fruiting body. Therefore, the present inventors have discovered that by increasing the carbon dioxide concentration and performing ear-growing management in the absence of light, brain-like fruiting bodies can be cultivated.

[0026] The embodiments of the present invention have the following advantages:

[0027] The invention provides a new white variant strain HZSW-Y7 of Auricularia auriculariae, realizes the artificial cultivation of the edible fungus, enriches the variety supply of the edible fungus in the market, and has high economic and social benefits.

[0028] The cultivation substrate of the white variant strain of Auricularia auricularia auriculariae is mainly corn cobs, and agricultural waste can be used to cultivate the brain white fungus, thereby realizing the utilization of agricultural waste resources and solving the problem of damage to deep forest wood resources.

[0029] The white variant strain of Auricularia auricularia auriculariae is produced in one wave, and the fruiting bodies are large, small in number, and high in yield, thereby realizing mechanized picking and greatly saving labor costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the embodiments or the description of the prior art. Obviously, the drawings described below are merely exemplary, and those skilled in the art can derive other implementation drawings based on the provided drawings without inventive effort.

[0031] FIG1 is a production picture of the white variant strain of Auricularia auricularia auricularia provided by the present invention. DETAILED DESCRIPTION

[0032] The following describes the implementation of the present invention using specific embodiments. Those skilled in the art will readily understand the other advantages and benefits of the present invention from the disclosure herein. Obviously, the embodiments described are only a portion of the present invention, not all of it. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are intended to fall within the scope of protection of the present invention.

[0033] Example 1

[0034] Acquisition and identification of a white variant strain of Auricularia auriculariae HZSW-Y7

[0035] (1) The wild Auricularia auricularia collected from Changbai Mountain area of ​​Jilin Province was isolated and domesticated to obtain the strain HZSW-Y7.

[0036] (2) Strain identification

[0037] 1. Morphological characteristics

[0038] Mycelium: The mycelium is white, uniform and consistent, growing flat on the culture medium, with neat edges of the colony.

[0039] Fruiting body: brain-shaped, pure white and shiny on the ridge, the color in the grooves is slightly darker than on the spine, diameter 8-15cm, thickness 5-10cm.

[0040] 2. Biological characteristics

[0041] Biological characteristics include the optimal carbon source, the optimal nitrogen source, the optimal culture temperature and the optimal culture pH. The test method for these four characteristics is to inoculate the strain into culture dishes with different carbon source culture media, different nitrogen source culture media, basal culture media and different pH culture media, observe the growth rate of mycelium and compare the growth of mycelium to determine its biological characteristics. The growth is determined by comparing the density and uniformity of the mycelium, and the number of different "+"s is usually used to indicate the strength of the mycelium growth.

[0042] 2.1 Screening of the optimal carbon source

[0043] The optimal carbon source was screened by comparing the growth rate and growth potential of the strains on different carbon source media.

[0044] The basic formula of the experiment is: 200g potatoes, 20g carbon source, 3g peptone, and 20g agar.

[0045] According to Table 1, the strain grew fastest in the glucose carbon source medium and the mycelium grew strongly, indicating that glucose was the most suitable carbon source.

[0046] Table 1 Carbon source screening test results

[0047] 2.2 Screening of the optimal nitrogen source

[0048] The optimal nitrogen source was screened by comparing the growth rate and growth potential of the strains on different nitrogen source media.

[0049] The basic formula of the experiment is: 200g potatoes, 20g glucose, 3g nitrogen source, and 20g agar.

[0050] According to Table 2, the strain grew fastest and had the strongest mycelial growth in the nitrogen source medium of peptone, indicating that peptone was the optimal nitrogen source.

[0051] Table 2 Nitrogen source screening test results

[0052] 2.3 Optimal culture temperature screening

[0053] The optimal culture temperature was screened by comparing the growth rate and growth potential of the strain at different temperatures, and the temperatures were set at 22°C, 24°C, 26°C, and 28°C.

[0054] The experimental formula is: 200g potatoes, 20g glucose, 3g peptone, and 20g agar.

[0055] According to Table 3, the strain grew fastest at 28°C, but its growth was poor. The second fastest growing temperature was 26°C, where its mycelium grew strongly. Combining these two factors, 26°C was determined to be the optimal culture temperature.

[0056] Table 3 Temperature screening test results

[0057] 2.4 Screening of optimal culture pH

[0058] The optimal culture medium pH was screened by comparing the growth rate and growth potential of the strain at different culture medium pH, and the culture medium pH was adjusted to pH 5.0, pH 5.5, pH 6.0, and pH 6.5 using 0.1 mol / L NaOH and HCl.

[0059] The experimental formula is: 200g potatoes, 20g glucose, 3g peptone, and 20g agar.

[0060] According to Table 4, the strain grew fastest and had the strongest mycelial growth in the medium with pH 6.0, indicating that pH 6.0 was the optimal pH.

[0061] Table 4 pH screening test results

[0062] Based on the above test results, it can be determined that the optimal carbon source for this strain is glucose, the optimal nitrogen source is peptone, the optimal culture temperature is 26°C, and the optimal culture pH is pH 6.0.

[0063] 3. Genetic characteristics

[0064] ITS sequencing identification

[0065] ITS sequencing was commissioned to Sangon Biotech (Shanghai) Co., Ltd. (hereinafter referred to as "Sangon") and included the following process:

[0066] Extraction of test strain DNA: Follow the instructions of the Kangwei Century Plant Genomic DNA Extraction Kit. Store the obtained test strain DNA at -20°C for later use.

[0067] PCR Amplification and System Establishment: The fungal ITS sequence universal primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS4 (5'-TCCTCCGCTTATTGATATGC-3') were used to amplify the sample. The PCR reaction system (25 μL) consisted of 2.5 μL of 10× Buffer, 2.5 μL of MgCl2, 0.6 μL of dNTPs, 1 μL of primer ITS1, 1 μL of primer ITS4, 5 μL of DNA template, 0.25 μL of Taq DNA polymerase, and 14.65 μL of ddH2O. PCR amplification conditions included 35 cycles of pre-denaturation at 94°C for 5 min, denaturation at 94°C for 30 s, annealing at 60°C for 30 s, extension at 72°C for 30 s, and post-extension at 72°C for 10 min. The mixture was stored at 4°C. The PCR product was detected by 1% agarose gel electrophoresis, and the qualified ITS sequence was sent to the Biotechnology Research Institute for sequencing. The ITS sequence of the test strain was shown in SEQ ID NO: 1, which was a 523bp DNA fragment. Sequence comparison in NCBI showed that the ITS sequence of the test strain had the highest similarity with the ITS sequence of Auricularia auricularia. The species relationship was identified, and it was found that the test strain belonged to the Auricularia auricularia group.

[0068] 4. Nutritional Content

[0069] This strain contains 7.3% crude protein, 2.7% crude fat, 6.56% polysaccharides, 6.22% unsaturated fatty acids, and 35.1% edible dietary fiber; it is rich in trace elements such as Se, Mn, Cu, Fe, Zn, and macroelements such as Ca, P, K, and Mg; and contains eight essential amino acids for the human body: isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine.

[0070] [Corrected on 23.02.2024 according to Rule 91] The strain was deposited and the deposited name is: White variant of hairy fungus (Auricularia cornea) HZSW-Y7, the depositor is: China General Microbiological Culture Collection Management Center, the deposit address is: No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, the deposit date is: June 12, 2023, the deposit number is CGMCC NO.40663, the biological classification name is horny fungus, and the Latin name is Auricularia cornea.

[0071] Example 2

[0072] Artificial cultivation method of white variant strain of Auricularia auricularia auricularia

[0073] 1. Preparation of mother culture: The mother culture is a strain obtained by tissue separation and culture of fruiting bodies collected directly from the wild.

[0074] Here are the steps:

[0075] (1) Dry the white variant fruiting bodies of Auricularia auricularia auricularia and set aside for later use.

[0076] (2) Prepare PDA test tube culture medium, scissors, tweezers, alcohol lamp, sterile culture dish, and 75% alcohol.

[0077] (3) In a clean bench, pour 10 ml of 75% alcohol into each of two sterile culture dishes, cut the fruiting bodies into small pieces of 2-3 mm in size, place the small pieces of tissue in the first culture dish and disinfect them for 1 min, use sterile tweezers to remove the small pieces of tissue from the first culture dish and place them in the second culture dish and disinfect them for another 1 min, use sterile tweezers to remove the disinfected small pieces of tissue and inoculate them into the PDA test tube culture medium.

[0078] (4) Place the inoculated PDA culture medium in an incubator at 25°C for 15 days, and remove bacteria contaminated by other bacteria during the incubation process.

[0079] (5) Recipe: 200g potatoes, 20g glucose, 18g agar powder, 1000ml water. Preparation method: Wash and peel the potatoes, cut them into slices of uniform thickness and put them into a pot. Add appropriate amount of water and boil the potato slices until they are easy to break. Filter the filtrate with gauze to obtain the filtrate. Dissolve the glucose and agar powder in cold water and add them to the filtrate. Continue heating and stirring until the culture medium just boils. Add 15ml of culture medium into each test tube, then seal it with cotton. Use a small high-pressure sterilizer to sterilize at 121℃ for 20-25min. After sterilization, when the test tube temperature drops to 60-70℃, place the inclined surface. The length of the culture medium inclined surface should be two-thirds of the test tube. The culture medium should be cooled and set aside.

[0080] (6) Place the cooled test tube culture medium and inoculation tools in a clean clean bench, turn on the UV lamp for sterilization for 30 minutes, and turn off the UV lamp 10 minutes after sterilization to prepare for inoculation. Wipe and disinfect your hands, inoculation tools, and mother culture test tubes with alcohol cotton, and sterilize the inoculation hook with the flame of an alcohol lamp. After the inoculation hook cools, start inoculation. Use the inoculation hook to divide the HZSW-Y7 mother culture in step (4) into 3mm*3mm bacterial blocks, pick the bacterial blocks and inoculate them into the newly prepared test tube culture medium in step (5), seal the mouth with cotton, and place it in a 26℃ incubator for 14-16 days to obtain the mother culture. During the incubation process, observe and remove the bacterial strains with poor growth every day, and retain the bacterial strains with strong mycelium growth and vigorous vitality.

[0081] 2. Preparation of liquid strains in shake flasks

[0082] Formula: 14g glucose, 4g soy powder, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate, 1000ml water.

[0083] Preparation method: First dissolve the soybean powder with hot water. After boiling the water, pour the dissolved soybean powder and other raw materials into the pot. Stop heating when the liquid boils. Put 600ml of liquid in a 1000ml triangular bottle, add 1 drop of defoaming agent, seal the bottle and sterilize it at 121℃ for 40 minutes. After cooling to room temperature, inoculate an appropriate amount of mother culture according to the sterile operation method, and then put it in a shaker and culture it at a temperature of 24-26℃ and a speed of 160 rpm for 7 days.

[0084] 3. Preparation of fermentation liquid strains

[0085] The culture medium comprises the following components: 0.6% brown sugar, 0.9% glucose, 0.45% soybean powder, 0.07% peptone, 0.1% potassium, 0.06% magnesium, 0.02% defoaming agent, and the balance is water.

[0086] Preparation method:

[0087] (1) Ingredients

[0088] For an 800L fermenter, weigh the ingredients according to the recipe. Pre-soak and stir all ingredients, except the defoamer, to ensure they are free of lumps. Add water to the fermenter, ensuring it contains 60% of the total liquid volume. Add the defoamer first, then the other ingredients, and top up the volume to 650L. Once all ingredients have been added, seal the tank.

[0089] (2) Sterilization

[0090] Heat the fermenter to 121-123°C and sterilize it at a pressure of 0.12-0.15 MPa for 60-90 minutes. After sterilization, lower the fermenter to 24-26°C and prepare for inoculation.

[0091] (3) Inoculation and culture

[0092] Under calm wind conditions, inoculate the shake flask liquid culture into the fermentation tank according to the aseptic operation method, adjust the air intake, maintain the tank pressure at 0.02-0.03MPa, control the ventilation speed at 5-15m / s, and the culture temperature at 24-26℃. It can be used after 6-7 days of culture.

[0093] 4. Making mushroom bags

[0094] Formula: 15% poplar sawdust, 75% corn cobs, 6% wheat bran, 2% soybean powder, 1% lime, and 1% gypsum.

[0095] (1) Ingredients, mixing and bagging

[0096] Weigh the ingredients according to the recipe. Pre-wet the corncobs one day in advance. Add the ingredients to a mixing pot and dry mix for 7-10 minutes. Add an appropriate amount of water and wet mix for 20-25 minutes. Once the pH reaches 8-9 and the moisture content reaches 57%-59%, bagging is possible. Use 160mm x 370mm heat-resistant polyethylene bags. Each bag should weigh between 13-1.4kg.

[0097] (2) Sterilization and cooling of bacteria bags

[0098] Place the bacteria bag filled with culture medium into the sterilizer for sterilization. Set the sterilization temperature to 105℃ for 30 minutes, 115℃ for 30 minutes, 121℃ for 90 minutes, and let it sit for 30 minutes. After the sterilization process is completed, open the cabinet door when the pressure of the sterilizer drops to 0. When the temperature in the sterilizer drops to 80℃, move the bacteria bag out of the sterilizer and place it in the cooling room for cooling. When the temperature of the bacteria bag drops to 28℃, prepare for inoculation.

[0099] (3) Inoculation of bacterial bags

[0100] In a clean inoculation room, use an automatic liquid inoculation machine to inoculate, with each package of fermentation liquid bacteria 25-30ml, and after inoculation, move it into the culture room for cultivation.

[0101] 5. Culture of bacterial bags

[0102] Before moving the bacteria bags into the incubation room, clean and disinfect the room before incubating. The incubation temperature for the bags is: 26-28°C for days 1-7, 23-24°C for days 8-20, and 24-25°C for days 20-35. Keep the carbon dioxide concentration between 800 and 1500 ppm. Incubate in the dark.

[0103] 6. Opening of mushroom bag

[0104] After the bags are incubated, they are moved to the fruiting room for opening. Opening methods include straight opening (2-3 cm), cross opening (2 cm x 2 cm), and piercing 10-30 microscopic holes within a 1.5 cm circle. Each bag is opened 5-20 times. After opening, the bags are incubated for 3-5 days to allow the mycelium to recover. Comparing different opening methods, the circular opening method (20 microscopic holes within a 1.5 cm diameter circle) yields the best fruiting results.

[0105] The results of different opening methods and fruiting body performance are shown in Table 5.

[0106] Table 5 Different hole opening methods and fruiting body performance results (each fungus bag has 10 holes)

[0107] The yield statistics of different opening numbers of 20 small holes pierced within a 1.5 cm circle are shown in Table 6.

[0108] Table 6 Output statistics of different opening numbers

[0109] The results showed that the average single bag yield and bioconversion rate were the best when the number of openings of the bag was 16.

[0110] 7. Put mushroom bags on shelves or hang bags

[0111] Fruiting method 1: Place the mushroom bags on the fruiting rack for single or double-sided fruiting, with a horizontal spacing of 2-10cm and a vertical spacing of 2-10cm.

[0112] The second fruiting method: hang the mushroom bags on the fruiting rack, open the sides of the bags to fruit the mushrooms, 5-8 bags in a bunch, and each bunch is 10-20cm apart.

[0113] 8. Germination management

[0114] Then increase the indoor humidity to between 70%-80%, maintain the temperature at 20-25℃, and control the carbon dioxide concentration between 800-1300ppm. Primordia will appear after 7-10 days.

[0115] 9. Ear management

[0116] Management from primordium to young mushroom stage: After the primordium appears, maintain the temperature at 20-25°C, CO2 at 1000-1500ppm, and humidity at 80%-90%. After 5-7 days, the primordium will grow and form young mushrooms.

[0117] Management from young mushrooms to mature mushrooms: After the young mushrooms are formed, maintain a temperature of 20-25°C, a carbon dioxide concentration of 1200-2000ppm, and a humidity of 80%-95%. They can be harvested after 10-15 days when the mushrooms reach maturity and a diameter of 5-8cm.

[0118] Each bag was pierced with 16 holes, using a circular opening pattern (20 small holes within a 1.5 cm circle). Five batches of cultivation were conducted using hanging bags. The weight and specifications of the fruiting bodies from different batches are shown in Table 7. The results demonstrate that the cultivation method provided by the present invention provides stable production performance and produces high-quality fruiting bodies.

[0119] Table 7 Statistical results of fruiting body weight and specifications of different batches of cultivation

[0120] Although the present invention has been described in detail above using general descriptions and specific embodiments, it will be apparent to those skilled in the art that modifications and improvements may be made thereto. Therefore, such modifications and improvements, without departing from the spirit of the present invention, are intended to be within the scope of protection claimed herein.

Claims

1. A white variant strain of Auricularia auricularia auricularia, characterized in that: The white variant strain of Auricularia cornea is Auricularia cornea HZSW-Y7, which was deposited in the China General Microbiological Culture Collection Center on June 12, 2023, with the deposit number CGMCC NO.40663.

2. The artificial cultivation method of the white variant strain of Auricularia auriculariae according to claim 1, characterized in that: The steps include: (1) inoculating the wild Auricularia auricularia fruiting body into a PDA medium for a first culture, and then transferring it to an original seed medium for a second culture to obtain a mother seed; (2) inoculating the original strain into a liquid culture medium for cultivation to obtain a shake flask liquid strain; (3) inoculating the liquid strain in the shake flask into a fermentation tank filled with fermentation medium for culturing to obtain a fermentation liquid strain; (4) Inoculate the fermented liquid bacteria into the fungus bag containing the culture medium, and then carry out the fungus bag cultivation, fungus bag opening, fungus bag placement or bag hanging, germination management, and earing management.

3. The artificial cultivation method of the white variant strain of Auricularia auriculariae according to claim 2, characterized in that: In step (1), The conditions of the first culture are: temperature 25°C, time 15 days; The formula of the original seed culture medium is: 200g potato, 20g glucose, 18g agar powder, 1000ml water; The conditions of the second culture are: temperature 26° C., time 14-16 days.

4. The artificial cultivation method of the white variant strain of Auricularia auriculariae according to claim 2, characterized in that: In step (2), The formula of the liquid culture medium is: 14g glucose, 4g soybean powder, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate, and 1000ml water; The culture conditions are: temperature 24-26° C., rotation speed 160 rpm, and time 7 days.

5. The artificial cultivation method of the white variant strain of Auricularia auricularia auriculariae according to claim 2, characterized in that: In step (3), The formula of the fermentation medium is: 0.6% brown sugar, 0.9% glucose, 0.45% soybean powder, 0.07% peptone, 0.1% potassium, 0.06% magnesium, 0.02% defoaming agent, and the balance is water; The culture conditions are as follows: adjusting the air intake, maintaining the tank pressure at 0.02-0.03 MPa, controlling the ventilation speed at 5-15 m / s, the temperature at 24-26° C., and the time for 6-7 days.

6. The artificial cultivation method of the white variant strain of Auricularia auricularia auriculariae according to claim 2, characterized in that: In step (4), the preparation method of the cultivation material is as follows: Weigh the following raw materials in percentage by weight: 15% poplar sawdust, 75% corn cobs, 6% wheat bran, 2% soybean powder, 1% lime, and 1% gypsum; Add all the raw materials into a stirring pot and dry-mix for 7-10 minutes. Add appropriate amount of water and wet-mix for 20-25 minutes. When the pH value of the raw materials reaches pH 8-9 and the water content reaches 57%-59%, they can be bagged.

7. The artificial cultivation method of the white variant strain of Auricularia auriculariae according to claim 6, characterized in that: The temperature program for culturing the bacterial bag is: 26-28° C. for 1-7 days, 23-24° C. for 8-20 days, 24-25° C. for 20-35 days, carbon dioxide concentration of 800-1500 ppm, and culturing under dark conditions.

8. The artificial cultivation method of the Auricularia auriculariae white variant strain Brain White Fungus according to claim 6, characterized in that: The size of the mushroom bag is 160mm*370mm, and the opening is a circular opening with a diameter of 1.5 centimeters. 20 micro holes are pierced within the circular range to form the circular opening. There are 16 openings on each mushroom bag. After the opening, the mushroom bag is cultured for 3 to 5 days to allow the mycelium to recover.

9. The artificial cultivation method of the white variant strain of Auricularia auriculariae according to claim 6, characterized in that: The germination management is carried out under dark conditions and includes: Increase the indoor humidity to between 70% and 80%, maintain the temperature at 20 to 25°C, and the carbon dioxide concentration at 800 to 1300 ppm. Primordia will appear after 7 to 10 days.

10. The artificial cultivation method of the white variant strain of Auricularia auriculariae according to claim 6, characterized in that: The ear management is carried out under dark conditions and includes: Management from primordium to young mushroom stage: After the primordium appears, keep the temperature at 20-25℃, the carbon dioxide concentration at 1000-1500ppm, and the humidity at 80%-90%. After 5-7 days, the primordium will grow and form young mushrooms. Management from young mushrooms to mature stage: After the young mushrooms are formed, maintain the temperature at 20-25℃, the carbon dioxide concentration at 1200-2000ppm, and the humidity at 80%-95%. The mushrooms will mature in 10-15 days and can be harvested when the diameter reaches 5-8cm.

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