Antimicrobial composition applicable to powder formulation

Abstract

The present invention relates to an antimicrobial composition applicable to a powder formulation, the antimicrobial composition containing a combination of caprylyl glycol, ethylhexylglycerin, and p-anisic acid, or a combination of 1,3-propanediol, methylpropanediol, and p-anisic acid. Due to containing a combination of specific components, when the antibacterial composition is applied to a powder formulation, the antibacterial composition exhibits appropriate antiseptic properties even at a low content, and the powder formulation has excellent stability because mold mycelium or moisture condensation is prevented from occurring even in a high humidity environment.

Description

Antibacterial composition applicable to powder formulations

[0001] It relates to an antibacterial composition applicable to powder formulations.

[0002] Powder formulations are considered a cosmetic product with a low risk of microbial growth because they do not contain water. However, if a tableted product is distributed or stored in a high-temperature, humid environment, it can present a surface for microbial growth, potentially leading to microbial contamination such as mold.

[0003] However, due to the nature of cosmetic preservatives that effectively exhibit antibacterial activity in water, the antibacterial raw materials applicable to powders are very limited, and very high concentrations must be used when used alone.

[0004] Therefore, it is necessary to discover preservative raw materials that can be applied to powder formulations that can effectively suppress the growth of microorganisms such as bacteria, yeast, and mold in various environments.

[0005] One aspect is to provide an antimicrobial composition applicable to a powder formulation, comprising caprylyl glycol, ethylhexylglycerin, and p-anisic acid.

[0006] Another aspect is to provide an antimicrobial composition applicable to a powder formulation, comprising 1,3-propanediol, methylpropanediol, and p-anisic acid.

[0007] Another aspect is to provide a cosmetic composition comprising an antimicrobial composition applicable to the above powder formulation.

[0008] Another aspect is to provide a composition for external application to the skin comprising an antibacterial composition applicable to the above powder formulation.

[0009] Another aspect is to provide a pharmaceutical composition comprising an antimicrobial composition applicable to the above powder formulation.

[0010] Another aspect provides the use of a combination of caprylyl glycol, ethylhexylglycerin, and p-anisic acid for preparing an antimicrobial composition applicable to a powder formulation.

[0011] Another aspect provides the use of a combination of 1,3-propanediol, methylpropanediol, and p-anisic acid for preparing an antimicrobial composition applicable to a powder formulation.

[0012] One aspect provides an antimicrobial composition applicable to a powder formulation, comprising caprylyl glycol, ethylhexylglycerin, and p-anisic acid.

[0013] The above antibacterial composition comprises a combination of caprylyl glycol, ethylhexylglycerin, and p-anisic acid.

[0014] The weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anisic acid may be 1 to 32: 1 to 16: 1 to 32, 1 to 2: 1 to 8: 1 to 32, 1 to 2: 2 to 4: 1, 1 to 8: 1: 1 to 32, 1 to 32: 1: 1 to 8, 1 to 8: 1 to 16: 1, 1 to 7: 1 to 5: 1 to 3, 4 to 6: 4 to 6: 2 to 4, 4 to 6: 4 to 6: 3, or 5: 5: 3. The weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anisic acid may be any range or any value selected from the above ranges. If the above weight ratio is outside the above range, the powder formulation may exhibit unsuitable preservative properties, or mold hyphae or moisture may form in the powder formulation, thereby reducing the stability of the formulation.

[0015] When the weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anisic acid is 1 to 2:1 to 8:1 to 32, an antibacterial synergistic effect against Escherichia coli (E. coli) can be exhibited. When the weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anisic acid is 1 to 2:2 to 4:1, an antibacterial synergistic effect against P. aeruginosa can be exhibited. When the weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anisic acid is 1 to 8:1:1 to 32, an antibacterial synergistic effect against Staphylococcus aureus (S. aureus) can be exhibited. When the weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anisic acid is 1 to 32: 1: 1 to 8, an antibacterial synergistic effect can be exhibited against yeast (C. albicans). When the weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anisic acid is 1 to 8: 1 to 16: 1, an antibacterial synergistic effect can be exhibited against mold (A. brasiliensis).

[0016] In one specific example, the caprylyl glycol is present in an amount of 0.004 to 5 wt%, 0.004 to 3 wt%, 0.004 to 1 wt%, 0.004 to 0.7 wt%, 0.004 to 0.6 wt%, 0.04 to 5 wt%, 0.04 to 3 wt%, 0.04 to 1 wt%, 0.04 to 0.7 wt%, 0.04 to 0.6 wt%, 0.1 to 5 wt%, 0.1 to 3 wt%, 0.1 to 1 wt%, 0.1 to 0.7 wt%, 0.1 to 0.6 wt%, 0.1 to 0.5 wt%, 0.1 to 0.4 wt%, 0.2 to 0.7 wt%, 0.2 to 0.6 %, 0.2 to 0.5 wt%, 0.2 to 0.4 wt%, 0.3 to 0.7 wt%, 0.3 to 0.6 wt%, 0.3 to 0.5 wt%, 0.3 to 0.4 wt%, 0.4 to 0.7 wt%, or 0.4 to 0.6 wt%. If the content of caprylyl glycol is outside the above range, problems such as microbial contamination due to weakening of antibacterial synergy between preservative ingredients or skin irritation due to excessive preservative content may occur.

[0017] In one specific example, based on the total weight of the composition, the ethylhexylglycerin is present in an amount of 0.004 to 5 wt%, 0.004 to 3 wt%, 0.004 to 1 wt%, 0.004 to 0.7 wt%, 0.004 to 0.6 wt%, 0.04 to 5 wt%, 0.04 to 3 wt%, 0.04 to 1 wt%, 0.04 to 0.7 wt%, 0.04 to 0.6 wt%, 0.1 to 5 wt%, 0.1 to 3 wt%, 0.1 to 1 wt%, 0.1 to 0.7 wt%, 0.1 to 0.6 wt%, 0.1 to 0.5 wt%, 0.1 to 0.4 wt%, 0.2 to 0.7 wt%, 0.2 to 0.6 %, 0.2 to 0.5 wt%, 0.2 to 0.4 wt%, 0.3 to 0.7 wt%, 0.3 to 0.6 wt%, 0.3 to 0.5 wt%, 0.3 to 0.4 wt%, 0.4 to 0.7 wt%, or 0.4 to 0.6 wt%. If the content of ethylhexylglycerin is outside the above range, problems such as microbial contamination due to weakening of antibacterial synergy between preservative ingredients or skin irritation due to excessive preservative content may occur.

[0018] In one specific example, the p-anisic acid may be included in an amount of 0.02 to 0.5 wt%, 0.02 to 0.4 wt%, 0.02 to 0.3 wt%, 0.1 to 0.5 wt%, 0.1 to 0.4 wt%, 0.1 to 0.3 wt%, 0.2 to 0.5 wt%, 0.2 to 0.4 wt%, or 0.2 to 0.3 wt% based on the total weight of the composition. If the content of p-anisic acid is outside the above range, problems such as microbial contamination due to weakened antibacterial synergy between preservative ingredients, skin irritation due to excessive preservative content, or weakened formulation stability due to precipitation of raw materials may occur.

[0019] When the above antibacterial composition is applied to a composition in a powder formulation (e.g., a cosmetic composition), the content of each of caprylyl glycol, ethylhexylglycerin, and p-anisic acid may be the content relative to the total weight of the composition in the powder formulation.

[0020] Another aspect provides an antimicrobial composition applicable to a powder formulation, comprising 1,3-propanediol, methylpropanediol, and p-Anisic Acid.

[0021] The above antibacterial composition comprises a combination of 1,3-propanediol, methylpropanediol, and p-anisic acid.

[0022] The weight ratio of the above 1,3-propanediol, methylpropanediol, and p-anisic acid may be 1 to 512:1 to 512:1, 2 to 128:1 to 32:1, 8 to 32:32 to 64:1, 1 to 128:1 to 128:1, 4 to 512:4 to 512:1, 8 to 256:4 to 256:1, 5 to 10:5 to 10:1 to 3, 4 to 6:9 to 11:2 to 4, 4 to 6:9 to 11:3, or 5:10:3. The weight ratio of the above 1,3-propanediol, methylpropanediol, and p-anisic acid may be any range or value selected from the above range. If the weight ratio is outside the above range, the powder formulation may exhibit unsuitable preservative properties, or mold hyphae or moisture condensation may occur in the powder formulation, thereby reducing the stability of the formulation.

[0023] When the weight ratio of the 1,3-propanediol, methylpropanediol, and p-anisic acid is 2 to 128:1 to 32:1, an antibacterial synergistic effect can be exhibited against Escherichia coli. When the weight ratio of the 1,3-propanediol, methylpropanediol, and p-anisic acid is 8 to 32:32 to 64:1, an antibacterial synergistic effect can be exhibited against P. aeruginosa. When the weight ratio of the 1,3-propanediol, methylpropanediol, and p-anisic acid is 1 to 128:1 to 128:1, an antibacterial synergistic effect can be exhibited against Staphylococcus aureus. When the weight ratio of the above 1,3-propanediol, methylpropanediol, and p-anisic acid is 4 to 512:4 to 512:1, an antibacterial synergistic effect against yeast (C. albicans) can be exhibited. When the weight ratio of the above 1,3-propanediol, methylpropanediol, and p-anisic acid is 8 to 256:4 to 256:1, an antibacterial synergistic effect against mold (A. brasiliensis) can be exhibited.

[0024] In one specific example, based on the total weight of the composition, the 1,3-propanediol is present in an amount of 0.25 to 32 wt%, 0.25 to 16 wt%, 0.25 to 8 wt%, 0.25 to 4 wt%, 0.25 to 2 wt%, 0.25 to 1 wt%, 0.25 to 0.8 wt%, 0.25 to 0.5 wt%, 0.3 to 32 wt%, 0.3 to 16 wt%, 0.3 to 8 wt%, 0.3 to 4 wt%, 0.3 to 2 wt%, 0.3 to 1 wt%, 0.3 to 0.8 wt%, 0.3 to 0.5 wt%, 0.35 to 32 wt%, 0.35 to 16 wt%, 0.35 to 8 wt%, It may be included at 0.35 to 4 wt%, 0.35 to 2 wt%, 0.35 to 1 wt%, 0.35 to 0.8 wt%, or 0.35 to 0.5 wt%. If the content of 1,3-propanediol is outside the above range, problems such as microbial contamination due to weakened antibacterial synergy between preservative ingredients or skin irritation due to excessive preservative content may occur.

[0025] In one specific embodiment, the methylpropanediol is present in an amount of 0.25 to 32 wt%, 0.25 to 16 wt%, 0.25 to 8 wt%, 0.25 to 4 wt%, 0.25 to 2 wt%, 0.25 to 1.5 wt%, 0.25 to 1 wt%, 0.5 to 32 wt%, 0.5 to 16 wt%, 0.5 to 8 wt%, 0.5 to 4 wt%, 0.5 to 2 wt%, 0.5 to 1.5 wt%, 0.5 to 1 wt%, 0.7 to 32 wt%, 0.7 to 16 wt%, 0.7 to 8 wt%, 0.7 to 4 wt%, 0.7 to 2 wt%, 0.7 to 1.5 wt%, or It may be included in an amount of 0.7 to 1 wt%. If the content of methylpropanediol exceeds the above range, problems such as microbial contamination due to weakened antibacterial synergy between preservative ingredients or skin irritation due to excessive preservative content may occur.

[0026] In one specific example, the p-anisic acid may be included in an amount of 0.02 to 0.5 wt%, 0.02 to 0.4 wt%, 0.02 to 0.3 wt%, 0.1 to 0.5 wt%, 0.1 to 0.4 wt%, 0.1 to 0.3 wt%, 0.2 to 0.5 wt%, 0.2 to 0.4 wt%, or 0.2 to 0.3 wt% based on the total weight of the composition. If the content of p-anisic acid is outside the above range, problems such as microbial contamination due to weakened antibacterial synergy between preservative ingredients, skin irritation due to excessive preservative content, or weakened formulation stability due to precipitation of raw materials may occur.

[0027] When the above antibacterial composition is applied to a composition in a powder formulation (e.g., a cosmetic composition), the content of each of the 1,3-propanediol, methylpropanediol, and p-anisic acid may be the content relative to the total weight of the composition in the powder formulation.

[0028] The antimicrobial composition according to the above aspects is applicable to powder formulations. The term "applicable to powder formulations" may mean that it can be used as a preservative in the final product of the powder formulation. Unlike general cosmetic preservatives that are used for the purpose of applying to cosmetics containing water, the antimicrobial composition according to the above aspects is applicable to powder formulations that do not contain water. The antimicrobial composition according to the above aspects comprises a combination of three specific ingredients, thereby exhibiting preservative properties suitable for powder formulations even at low contents. In addition, a powder formulation product to which the antimicrobial composition according to the above aspects is applied does not develop mold hyphae or moisture condensation even when distributed or stored in a high-temperature and high-humidity environment.

[0029] As used herein, the term “antimicrobial composition” means a composition that kills or inhibits the growth of microorganisms (e.g., bacteria, yeast, mold, etc.), and may be used interchangeably with the term “preservative composition” or “antiseptic composition.”

[0030] The antibacterial composition according to the above aspects can exhibit antibacterial activity against one or more selected from bacteria, yeast, and mold.

[0031] The above bacteria may include, but are not limited to, one or more species selected from Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus.

[0032] The yeast may include, but is not limited to, Candida albicans.

[0033] The above mold may include, but is not limited to, Aspergillus brasiliensis.

[0034] A powder formulation product to which an antimicrobial composition according to the above aspects is applied does not cause mold growth or moisture condensation even when distributed or stored in a high-temperature and humid environment. The high-temperature and humid environment may refer to, for example, a condition of a relative humidity of 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, or 90% or more and a temperature of 25°C or more, 30°C or more, 32°C or more, 35°C or more, or 25°C to 40°C. In one embodiment, it was confirmed that when a cosmetic composition to which an antimicrobial composition according to the above aspects is applied in a powder formulation was incubated at a temperature of 25°C (room temperature) or 32°C (high temperature) for 28 days at a relative humidity of 95%, mold growth and moisture condensation did not occur.

[0035] Another aspect provides a cosmetic composition comprising an antibacterial composition applicable to a powder formulation according to the above aspect.

[0036] The above cosmetic composition is not particularly limited to a specific formulation, and the formulation may be appropriately selected depending on the intended purpose. In one specific example, the cosmetic composition may be in a powder formulation.

[0037] The above cosmetic composition may additionally include ingredients commonly used in cosmetic compositions, functional additives, etc., and may include conventional auxiliary agents such as antioxidants, stabilizers, vitamins, pigments, fragrances, etc., and carriers.

[0038] Another aspect provides a composition for external application to the skin comprising an antibacterial composition applicable to a powder formulation according to the above aspect.

[0039] In one specific example, the external skin composition may be in powder form.

[0040] The above skin external preparation may be appropriately mixed with ingredients commonly used in skin external preparations such as cosmetics, medicines, and quasi-drugs, such as powder ingredients, ultraviolet absorbers, whitening agents, antioxidants, fragrances, coloring agents, various skin nutrients, metal ion sequestrants, sugars, or combinations thereof, as needed.

[0041] Another aspect provides a pharmaceutical composition comprising an antimicrobial composition applicable to a powder formulation according to the above aspect.

[0042] The above antibacterial composition can be included as an active ingredient of a pharmaceutical composition having antibacterial activity, or used as an auxiliary agent of an active ingredient having a pharmaceutical use.

[0043] In one specific embodiment, the pharmaceutical composition may be in powder form.

[0044] Another aspect provides the use of a combination of caprylyl glycol, ethylhexylglycerin, and p-anisic acid for preparing an antimicrobial composition applicable to a powder formulation.

[0045] Another aspect provides the use of a combination of 1,3-propanediol, methylpropanediol, and p-anisic acid for preparing an antimicrobial composition applicable to a powder formulation.

[0046] Duplicate content is omitted in consideration of the complexity of this specification, and terms not otherwise defined herein have the meanings commonly used in the technical field to which the present invention belongs.

[0047] An antibacterial composition according to one aspect comprises a combination of caprylyl glycol, ethylhexylglycerin, and p-anisic acid; or a combination of 1,3-propanediol, methylpropanediol, and p-anisic acid, thereby exhibiting suitable preservative properties even at low contents when applied to a powder formulation, and exhibiting excellent stability as no mold hyphae or moisture condensation occurs in the powder formulation in a high humidity environment. Therefore, the antibacterial composition can be used as a preservative in powder formulation cosmetics, external skin preparations, etc.

[0048] Figure 1 is a photograph showing the results of incubating a powder formulation (Sample No. 3-2) containing a combination of caprylyl glycol, ethylhexylglycerin, and p-anisic acid as antibacterial raw materials in a high humidity environment.

[0049] Figure 2 is a photograph showing the results of incubating a powder formulation (Sample No. 4-3) containing a combination of 1,3-propanediol and methylpropanediol as antibacterial raw materials in a high humidity environment.

[0050] Figure 3 is a photograph showing the results of incubating a powder formulation (Sample No. 4-4) containing a combination of 1,3-propanediol and methylpropanediol as antibacterial raw materials in a high humidity environment.

[0051] Figure 4 is a photograph showing the results of incubating a powder formulation (sample No. 4-5) containing a combination of 1,3-propanediol and methylpropanediol as antibacterial raw materials in a high humidity environment.

[0052] Figure 5 is a photograph showing the results of incubating a powder formulation (Sample No. 5-2) containing a combination of 1,3-propanediol, methylpropanediol, and p-anisic acid as antibacterial raw materials in a high humidity environment.

[0053] The following examples are provided for more detailed description. However, these examples are provided solely to illustrate one or more specific examples, and the scope of the present invention is not limited to these examples.

[0054] Example 1: Confirmation of antibacterial synergy effect

[0055] The three-dimensional antimicrobial synergy of the combinations of caprylyl glycol, ethylhexylglycerin, and p-anisic acid and 1,3-propanediol, methylpropanediol, and p-anisic acid was confirmed. The specific antimicrobial synergy test method is as follows. The concentration of the raw materials was based on the minimum inhibitory concentration (MIC) of each raw material.

[0056] When testing for bacteria, dilute with Tryptic Soy Agar (TSA), and when testing for fungi, dilute with Sabouraud Dextrose Agar (SDA) to the desired concentration. To confirm the minimum inhibitory concentration of the raw material, add 100 ㎕ of the bacterial solution with the adjusted number of bacteria to a 96-well plate. Add 100 ㎕ of the prepared raw material to the first row of the plate containing 100 ㎕ of the bacterial solution, mix the bacterial solution and the raw material well, and then add 100 ㎕ to the second row so that the raw material is diluted by 1 / 2. After diluting the raw material by 1 / 2 in this manner, discard the 100 ㎕ mixed solution remaining in the last row. After plate culture, check the turbidity of the medium to determine visually whether the bacteria have grown. The lowest concentration at which no bacteria grew, as determined by turbidity, is defined as the minimum inhibitory concentration (MIC) for the raw material. The minimum inhibitory concentrations (weight %) of the raw materials for each strain measured in this test are shown in Table 1.

[0057] For the purpose of confirming the antibacterial synergy effect, raw materials A (caprylyl glycol or 1,3-propanediol), B (ethylhexylglycerin or methylpropanediol), and C (p-anisic acid) are prepared to 8 times the minimum inhibitory concentration. By diluting the prepared raw materials by 1 / 2 in steps using tryptic soy medium, raw materials A and B are prepared from 8 times the minimum inhibitory concentration to 1 / 8. Raw material C is prepared from 8 times the minimum inhibitory concentration to 1 time.

[0058] Dispense 25 ㎕ of type A raw material into the wells of the X-axis row of a 96-well plate in increasing concentrations (1 / 8 of the minimum inhibitory concentration) to high concentrations (8 times the minimum inhibitory concentration). Dispense 25 ㎕ of type B raw material into the wells of the Y-axis column in increasing concentrations (1 / 8 of the minimum inhibitory concentration) to high concentrations (8 times the minimum inhibitory concentration). Dispense 25 ㎕ of type C raw material into the wells of the Z-axis plate of a 96-well plate in increasing concentrations (minimum inhibitory concentration) to high concentrations (8 times the minimum inhibitory concentration). Dispense 25 ㎕ of each bacterial solution with the same number of bacteria into all wells containing the raw material and culture.

[0059] A 96-well plate containing E. coli, P. aeruginosa, and S. aureus strains and diluted raw materials is cultured in a 32°C incubator for 24 hours, and a 96-well plate containing C. albicans and A. brasiliensis strains and diluted raw materials is cultured in a 25°C incubator for 48 hours. Then, the mixing ratio (weight ratio) of the minimum concentration at which bacterial growth is not observed with the naked eye is confirmed.

[0060] To confirm the most efficient mixing concentration results, the fractional inhibiting concentration (FIC) and FIC index are calculated by substituting them into the following mathematical formula.

[0061] [Mathematical Formula 1]

[0062] FIC of substance A, B, or C = (MIC of raw material A, B, or C in the mixture) / (MIC when raw material A, B, or C is used alone)

[0063] [Equation 2]

[0064] FIC index = FIC of substance A + FIC of substance B + FIC of substance C

[0065] If the range where the fungus does not proliferate is FIC index ≤ 0.5, it is considered to have a synergistic effect; if 0.5 < FIC index ≤ 1, it is considered to have a partial synergistic effect; if 1 < FIC index ≤ 4, it is considered to have an indifferent effect; and if FIC index > 4, it is considered to have an antagonistic effect.

[0066] In this test, caprylyl glycol was used at 0.004 to 0.5 wt%, ethylhexylglycerin at 0.004 to 1 wt%, 1,3-propanediol and methylpropanediol at 0.25 to 32 wt%, and p-anisic acid at 0.02 to 0.5 wt% based on the minimum inhibitory concentration content of each raw material. The results of the antibacterial synergy test of the combination of caprylyl glycol, ethylhexylglycerin, and p-anisic acid and the combination of 1,3-propanediol, methylpropanediol, and p-anisic acid are shown in Table 2 below as the weight ratios showing the synergy between the raw materials.

[0067] <Abbreviation>

[0068] CG: Caprylyl glycol

[0069] EHG: Ethylhexylglycerin

[0070] p-AA: p-Anisic acid

[0071] 1,3-PD: 1,3-Propanediol

[0072] MPD: Methylpropanediol

[0073]

[0074]

[0075]

[0076]

[0077]

[0078] As shown in Table 2 above, the results of the antibacterial synergy test against bacteria showed that the combination of caprylyl glycol, ethylhexylglycerin, and p-anisic acid exhibited antibacterial synergy against Escherichia coli (E. coli) at a weight ratio of 1 to 2:1 to 8:1 to 32, antibacterial synergy against Pseudomonas aeruginosa at a weight ratio of 1 to 2:2 to 4:1, and antibacterial synergy against Staphylococcus aureus (S. aureus) at a weight ratio of 1 to 8:1:1 to 32. It exhibited antibacterial synergy against yeast (C. albicans) at a weight ratio of 1 to 32:1:1 to 8. It exhibited antibacterial synergy against mold (A. brasiliensis) at a weight ratio of 1 to 8:1 to 16:1 to 16. The combination of 1,3-propanediol, methylpropanediol and p-anisic acid exhibited antibacterial synergy against bacteria at a weight ratio of 2 to 128:1 to 32:1, against Pseudomonas aeruginosa at a weight ratio of 8 to 32:32 to 64:1, and against Staphylococcus aureus at a weight ratio of 1 to 128:1 to 128:1. Against yeast, it exhibited antibacterial synergy at a weight ratio of 4 to 512:4 to 512:1. For fungi, antibacterial synergy was shown at a weight ratio of 8 to 256:4 to 256:1.

[0079] Example 2: Buoyancy test

[0080] A preservative test was performed on a powder formulation cosmetic composition containing an antibacterial composition.

[0081] First, a cosmetic composition in powder form containing the antibacterial ingredients (wt%) shown in Table 3 below was prepared. The cosmetic composition in powder form was prepared by a general method of crushing a powder phase to prepare a powder phase, placing the powder phase in a Henschel mixer, spraying and mixing the oil phase into the Henschel mixer, and then compression molding. Specifically, 70 to 80 wt% of the powder base material and 5 to 10 wt% of the pigment were placed in a mixer and mixed at 1200 to 1500 rpm for 1 to 2 minutes. Thereafter, 10 to 20 wt% of the oil binder was mixed by heating to 65 to 85°C, and then sprayed directly onto the mixed powder phase while stirring at 200 to 400 rpm, and then mixed. The powder base is in the form of a powder and comprises at least one selected from among talc, titanium dioxide, mica, silica, nylon powder, aluminum starch octenylsuccinate, magnesium myristate, magnesium stearate, lauroyl lysine, triethoxycaprylylsilane, and p-anisic acid. The binder is in the form of an oil and comprises at least one selected from among caprylic / capric triglyceride, triethylhexanoin, polyglyceryl-2 triisostearate, hydrogenated castor oil isostearate, caprylyl glycol, ethylhexylglycerin, propanediol, and methylpropanediol. The pigment comprises at least one selected from among yellow iron oxide, red iron oxide, black iron oxide, sulfide selenide, ferrocyanide, carmine, and manganese violet.

[0082] Next, a cosmetic preservative test was performed on the cosmetic composition of the powder formulation manufactured above. Specifically, the test was performed on Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, and Staphylococcus aureus ATCC 6538 among bacteria; Candida albicans ATCC 10231 among yeasts; and Aspergillus brasiliensis ATCC 16404 among molds. The bacterial (Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, and Staphylococcus aureus ATCC 6538) inoculum was incubated with 0.85% NaCl until the number of each inoculum was 10 8 CFU / ml was adjusted and mixed, and the yeast (Candida albicans ATCC 10231) and mold (Aspergillus brasiliensis, ATCC 16404) were each mixed at a count of 10 7 It was adjusted to be CFU / ml. 1% bacterial, yeast, and mold solutions were added to 20 g of the prepared powder formulation, mixed, and cultured at 25℃ for 4 weeks. The number of bacteria remaining in the 1st, 2nd, and 4th weeks was measured and converted to a log value to confirm the rate of bacterial reduction.

[0083] The results of the preservative test are shown in Table 3 below.

[0084] <Abbreviation>

[0085] CG: Caprylyl glycol

[0086] EHG: Ethylhexylglycerin

[0087] p-AA: p-Anisic acid

[0088] 1,3-PD: 1,3-Propanediol

[0089] MPD: Methylpropanediol

[0090]

[0091] As shown in Table 3 above, p-anisic acid alone (Samples No. 1-1 to 1-3) or a combination of caprylyl glycol and ethylhexylglycerin (Samples No. 2-1 to 2-3) exhibited inadequate preservative properties in powder formulations. A combination of 1,3-propanediol and methylpropanediol (Samples No. 4-1 and 4-2) exhibited adequate preservative properties in powder formulations, but as can be seen in Example 3 below, mold mycelia grew in the powder formulations or moisture condensation occurred in high humidity environments, resulting in poor stability.

[0092] In contrast, the combination of caprylyl glycol, ethylhexylglycerin, and p-anisic acid (Sample No. 3-1) or the combination of 1,3-propanediol, methylpropanediol, and p-anisic acid (Sample No. 5-1) exhibited suitable preservative properties in powder formulations even at low contents.

[0093] Example 3: Mold Test and Stability Evaluation

[0094] A fungal test and stability evaluation were performed on a powder formulation cosmetic composition containing an antibacterial composition.

[0095] First, a cosmetic composition in powder form containing the antibacterial ingredients (weight %) shown in Table 4 below was prepared. The method for preparing the cosmetic composition in powder form was the same as in Example 2.

[0096] Next, a mold test and stability evaluation were performed on the cosmetic composition of the powder formulation manufactured above. Specifically, 10 6Inoculate 3 μL of the fungal solution adjusted to CFU / mL onto one or more sites. After incubation for 28 days under the incubation conditions shown in Table 3 below, the presence of fungal hyphae and moisture condensation was checked.

[0097] The occurrence of fungal hyphae was visually confirmed, and the results were indicated as follows according to the degree of occurrence: -: No hyphae at the inoculation site, +: No hyphae visible, but spores spread around the inoculation site, ++: A small amount of hyphae is visible around the inoculation site and spores spread, +++: A large amount of hyphae is confirmed around the inoculation site.

[0098] The moisture condensation phenomenon was confirmed with the naked eye, and if moisture condensation occurred, it was judged as failing in the stability category.

[0099]

[0100] As shown in Table 4 and FIGS. 1 to 5, the combination of 1,3-propanediol and methylpropanediol (Samples No. 4-3 to 4-5) exhibited suitable preservative properties in powder formulations, but had low safety and low stability due to occurrence of mold mycelia or moisture condensation in a high-humidity environment. On the other hand, the combination of caprylyl glycol, ethylhexylglycerin, and p-anisic acid (Sample No. 3-2) or the combination of 1,3-propanediol, methylpropanediol, and p-anisic acid (Sample No. 5-2) did not cause mold mycelia or moisture condensation in the powder formulations in a high-humidity environment, and thus it was confirmed that they had excellent safety and excellent stability.

Claims

1. An antibacterial composition applicable to a powder formulation, comprising caprylyl glycol, ethylhexylglycerin, and p-anisic acid.

2. An antibacterial composition applicable to a powder formulation, wherein the weight ratio of caprylyl glycol, ethylhexylglycerin, and p-anisic acid in claim 1 is 1 to 32:1 to 16:1 to 32.

3. In claim 1, with respect to the total weight of the composition, The above caprylyl glycol is contained in an amount of 0.004 to 5 wt%, The above ethylhexylglycerin is contained in an amount of 0.004 to 5 wt%, and An antibacterial composition applicable to a powder formulation, wherein the above p-anisic acid is contained in an amount of 0.02 to 0.5 wt%. An antimicrobial composition applicable to a powder formulation, comprising 4.1,3-propanediol, methylpropanediol, and p-anisic acid.

5. An antibacterial composition applicable to a powder formulation, wherein the weight ratio of 1,3-propanediol, methylpropanediol, and p-anisic acid in claim 4 is 1 to 512:1 to 512:

1.

6. In claim 4, with respect to the total weight of the composition, The above 1,3-propanediol is contained in an amount of 0.25 to 32 wt%, The above methylpropanediol is contained in an amount of 0.25 to 32 wt%, and An antibacterial composition applicable to a powder formulation, wherein the above p-anisic acid is contained in an amount of 0.02 to 0.5 wt%.

7. An antimicrobial composition applicable to a powder formulation, which exhibits antimicrobial activity against at least one selected from bacteria, yeast, and mold according to claim 1 or 4.

8. In claim 7, The above bacteria include at least one selected from Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, The above yeast includes Candida albicans, An antimicrobial composition applicable to a powder formulation, wherein the mold comprises Aspergillus brasiliensis.

9. A cosmetic composition comprising an antibacterial composition applicable to the powder formulation of claim 1 or 4.

10. A composition for external use in the skin comprising an antibacterial composition applicable to the powder formulation of claim 1 or 4.

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