Systems and compositions for use in treating presbyopia

Abstract

The present disclosure provides aqueous compositions comprising aceclidine that are stable at room temperature for prolonged periods of time. The present disclosure also provides systems comprising a container, such as a single use container, and an ophthalmological composition, such as comprising aceclidine. Also provided are methods of using such systems, containers, and compositions, such as in the treatment of ocular disorders (e.g., presbyopia).

Description

SYSTEMS AND COMPOSITIONS FOR USE IN TREATING PRESBYOPIACROSS REFERENCE TO RELATED APPLICATIONS[1] This application is a continuation-in-part of U.S. Application No. 18 / 939,325, filed November 6, 2024, and U.S. Application No. 18 / 606,372, filed on March 15, 2024, and claims priority to and the benefit of U.S. Application No. 18 / 939,325, filed November 6, 2024, U.S. Application No. 18 / 606,372, filed on March 15, 2024, and U.S. Provisional Application Nos. 63 / 565,668, filed on March 15, 2024, 63 / 573,626, filed on April 03, 2024, 63 / 660,858, filed on June 17, 2024, 63 / 565,672, filed on March 15, 2024, 63 / 573,630, filed on April 03, 2024, 63 / 660,853, filed on June 17, 2024, 63 / 565,674, filed on March 15, 2024, 63 / 573,632, filed on April 03, 2024, 63 / 660,848, filed on June 17, 2024, 63 / 565,679, filed on March 15, 2024, 63 / 573,636, filed on April 03, 2024, 63 / 660,969, filed on June 17, 2024, 63 / 565,687, filed on March 15, 2024, 63 / 573,640, filed on April 03, 2024, 63 / 660,962, filed on June 17, 2024, 63 / 565,692, filed on March 15, 2024, 63 / 573,641, filed on April 03, 2024, and 63 / 660,959, filed on June 17, 2024, each of which is hereby incorporated by reference herein in its entirety.BACKGROUND[2] Presbyopia is a type of refractive error that causes close objects to gradually appear out of focus as the eye ages. Presbyopia affects about 26% of the world's population, and treatment options include: corrective eyeglasses or contact lenses, refractive surgery, and lens implants. Most adults will experience presbyopia at some point in their lives, such as after reaching 40 years old.[3] In addition, according to the American Optometric Association (AO A), our eyes were not designed to use computers and digital screens especially for long periods of time. The eye focusing and coordination requirements can make the eyes work too hard. In some instances, prolonged screen usage can result in eye-related symptoms including eyestrain, headaches, dry eyes, and blurred vision. The level of discomfort appears to increase with the amount of electronic device use. Uncorrected vision problems can increase the severity of symptoms.[4] Additionally, contact lens discomfort can eventually lead to discontinuation of contact lens wear. It has been reported that the discontinuation, or dropout, of contact lenses ranges from 15% to 23% of wearers.SUMMARY[5] Disclosed herein, in some aspects, is a system comprising a single-patient-use container and an ophthalmological composition, wherein: the ophthalmological composition comprises aceclidine or a salt thereof; and the single-patient-use container comprises an enclosed chamber,the ophthalmological composition being configured within the enclosed chamber, the singlepatient-use container being configured to be irreversibly opened, and the single-patient-use container being configured, when open, to dispense a first drop and a second drop of the ophthalmological composition through an opening.[6] In some embodiments, the single-patient-use container is a vial or an ampoule. In some embodiments, the single-patient-use container comprises a container body, a container neck, and a container head, the container body and the container head having a larger cross-sectional dimension than the container neck, the enclosed chamber extending from the container body into the container neck, and the single-patient-use container being configured to be opened at the container neck. In some embodiments, prior to opening, the enclosed chamber is sterile. In some embodiments, the container is transparent. In some embodiments, the container comprises polyethylene. In some embodiments, the container comprises a low-density polyethylene (LDPE). In some embodiments, the system comprises about 0.2 m to about 2 mL of the ophthalmological composition. In some embodiments, the system comprises about 0.2 mL to about 1 mL of the ophthalmological composition. In some embodiments, the system comprises about 0.5 mL of the ophthalmological composition. In some embodiments, the ophthalmological composition comprises aceclidine or a salt thereof in a concentration of about 1 wt. % to about 2 wt. %. In some embodiments, the ophthalmological composition comprises aceclidine or a salt thereof in a concentration of about 1.75 wt. %. In some embodiments, the ophthalmological composition further comprises brimonidine. In some embodiments, the ophthalmological composition comprises brimonidine or a salt thereof in a concentration of about 0.05 wt. % to about 0.15 wt. %. In some embodiments, the composition comprises brimonidine or a salt thereof in a concentration of about 0.08 wt. %. In some embodiments, the ophthalmological composition comprises aceclidine hydrochloride in a concentration of about 1.75 wt. % and brimonidine tartrate in a concentration of about 0.08 wt. %. In some embodiments, the system comprises a package and at least 3 identical containers and at least 3 identical ophthalmological compositions[7] Disclosed herein, in some aspects, is a method for treating presbyopia in an individual (e.g., in need thereof), the method comprising: opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition, wherein the second drop is administered about 2 minutes after the first drop is administered to the first eye, wherein the ophthalmological composition comprises aceclidine or a salt thereof.[8] In some embodiments, the method further comprises administering a third drop and a fourth drop of the ophthalmological composition to a second eye of the individual, wherein the fourth drop is administered to the second eye about 2 minutes after the third drop is administeredto the second eye. In some embodiments, the third drop is administered to the second eye prior to the second drop being administered to the first eye. In some embodiments, the method further comprises opening a second single-patient-use container on a second day and subsequently administering to the first eye of the individual a second container first drop and a second container second drop of a second container ophthalmological composition, wherein the second container ophthalmological composition comprises aceclidine or a salt thereof, the second container second drop is administered to the first eye of the individual 2 minutes after the second container first drop is administered to the first eye, and the first and second days are different days. In some embodiments, the ophthalmological composition is an ophthalmological composition of any one of the preceding embodiments. In some embodiments, the second container ophthalmological composition is an ophthalmological composition of any one of the preceding embodiments. In some embodiments, the ophthalmological composition and the second container ophthalmological composition are identical.[9] Disclosed herein, in some aspects, is a method for treating presbyopia and improving distance vision in an individual (e.g., in need thereof), the method comprising: opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition, wherein the second drop is administered about 2 minutes after the first drop is administered to the first eye, wherein the ophthalmological composition comprises aceclidine or a salt thereof.

[0010] In some embodiments, the method further comprising administering to a second eye of the individual a third drop and a fourth drop of the ophthalmological composition, wherein the fourth drop is administered to the second eye about 2 minutes after the third drop is administered to the second eye. In some embodiments, the third drop is administered to the second eye prior to the second drop being administered to the first eye. In some embodiments, the method further comprising opening a second single-patient-use container on a second day and subsequently administering to the first eye of the individual a second container first drop and a second container second drop of a second container ophthalmological composition, wherein the second container ophthalmological composition comprises aceclidine or a salt thereof, the second container second drop is administered to the first eye of the individual 2 minutes after the second container first drop is administered to the first eye, and the first and second days are different days. In some embodiments, the ophthalmological composition is the ophthalmological composition of any one of claims 1-17. In some embodiments, the second container ophthalmological composition is the ophthalmological composition of any one of claims 1-17. In some embodiments, the ophthalmological composition and the second container ophthalmological composition are identical. In some embodiments, the individual has moderate presbyopia. In some embodiments,the individual has advanced presbyopia. In some embodiments, the individual has an improvement in near vision (e.g., visual acuity at 40 cm) of at least 4 lines. In some embodiments, the individual has an improvement in near vision (e g., visual acuity at 40 cm) of at least 5 lines. In some embodiments, the individual has an improvement in near vision (e.g., visual acuity at 40 cm) of at least 6 lines. In some embodiments, the individual has an improvement in distance vision (e g., visual acuity at 4 m) of at least 1 line. In some embodiments, the individual has an improvement in distance vision (e.g., visual acuity at 4 m) of at least 3 letters. In some embodiments, the individual is at least 60 years old. In some embodiments, the individual is 61-65 years old. In some embodiments, the individual is 66-70 years old.

[0011] Provided in certain embodiments herein are systems, compositions, and methods, such as for treating ocular disorders, such as presbyopia. In some embodiments, provided herein is a method of improving vision. In some embodiments, provided herein is a method of improving near vision. In some embodiments, provided herein is a method of improving distance vision. In some embodiments, provided herein is a method of treating presbyopia. In some embodiments, a composition provided herein comprises a composition comprising a miotic (e.g., aceclidine or a salt thereof). In some embodiments, a system provided herein comprises such a composition (e g., and a vessel containing the composition). In some embodiments, a method provided herein comprises administering such a composition, such as to an eye (e.g., surface) of an individual.

[0012] Provided in some embodiments herein is a method of treating presbyopia in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0013] Provided in some embodiments herein is a method of treating presbyopia with a concomitant improvement in myopia in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of treating presbyopia with a concomitant improvement in myopia in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0014] Provided in some embodiments herein is a method of treating presbyopia with a concomitant improvement in distance vision in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of treating presbyopia with a concomitant improvement in distance vision in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0015] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising administering to a first eye of the individual a first drop of an ophthalmic composition comprising aceclidine or a salt thereof. In some embodiments, the method comprisesadministering to the first eye a second drop of the ophthalmic composition. In specific embodiments, the second drop is administered to the first eye about 2 minutes after the first drop is administered to the first eye. In some embodiments, the individual previously had ocular surgery in the first eye. In specific embodiments, the ocular surgery is laser assisted in situ keratomileusis (LASIK) surgery or photorefractive keratectomy (PRK) surgery.

[0016] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmic composition to an eye of the individual. In specific embodiments, the ophthalmic composition comprising a miotic (e.g., pupil selective miotic, such as relative to ciliary muscle). In some embodiments, the eye of the individual having previously undergone ocular surgery.

[0017] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmic composition to an eye of the individual. In specific embodiments, the ophthalmic composition comprising aceclidine or a salt thereof. In some embodiments, the eye of the individual having previously undergone ocular surgery.

[0018] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising administering a first drop of an ophthalmic composition (such as described herein) to an eye of the individual. In some embodiments, the method comprises subsequently administering a second drop of the ophthalmic composition to the eye of the individual. In some embodiments, the ophthalmic composition comprises aceclidine or a salt thereof. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0019] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method consisting essentially of administering a first drop of an ophthalmic composition (such as described herein) to an eye of the individual. In some embodiments, the method comprises subsequently administering a second drop of the ophthalmic composition to the eye of the individual. In some embodiments, the ophthalmic composition comprises aceclidine or a salt thereof. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0020] Provided in some embodiments herein is a method of treating presbyopia and improving distance vision in an individual, the method comprising administering a first drop of an ophthalmic composition (such as described herein) to an eye of the individual and subsequently administering a second drop of the ophthalmic composition to the eye of the individual. In some embodiments, the ophthalmic composition comprises aceclidine or a salt thereof. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0021] Provided in some embodiments herein is a method of providing a pupil size of an eye of an individual to less than 2 millimeters (mm) in the eye of the individual (e.g., for at least 9 hours), the method comprising administering an ophthalmic composition comprising a miotic agent (such as described herein) to an eye of the individual.

[0022] Provided in some embodiments herein is a method for treating presbyopia, the method comprising instilling one drop of an ophthalmic composition comprising aceclidine or a salt thereof in each eye followed by a second drop in each eye about two (2) minutes later. In some embodiments this provides an effect of at least 10 hours (e.g., an improvement in near vision, distance vision, and / or visual acuity, as disclosed herein).

[0023] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising administering to a first eye of the individual a first drop of an ophthalmic composition comprising aceclidine or a salt thereof. In some embodiments, the method comprises administering to the first eye a second drop of the ophthalmic composition (such as described herein). In some embodiments, the second drop is administered to the first eye about 2 minutes after the first drop is administered to the first eye.

[0024] Provided in some embodiments herein is a method of treating presbyopia in a first eye of an individual, the method consisting essentially of administering to a first eye of the individual a first drop of an (e.g., a sterile, preservative-free) ophthalmic composition comprising aceclidine or a salt thereof in a concentration of about 1 wt. % to about 2 wt. %. In some embodiments, the method comprises administering to the first eye a second drop of the ophthalmic composition (such as described herein). In some embodiments, the second drop is administered to the first eye about 2 minutes after the first drop is administered to the first eye.

[0025] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmic composition (such as described herein) to an eye of the individual. In some embodiments, the composition comprises aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, the composition has a room temperature when administered to the eye of the individual.

[0026] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmic composition (such as described herein) to an eye of the individual. In some embodiments, the composition comprises aceclidine and has a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, the composition has been stored at room temperature for up to 6 months prior to administration. In some embodiments, the ophthalmic composition remains ophthalmically acceptable and suitable for treating presbyopia when administered to the eye for at least 6 months at room temperature.

[0027] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising storing an ophthalmic composition (such as described herein) at a temperature of room temperature for up to 14 days (e.g., up to 3 months) In some embodiments, the method comprises administering the ophthalmic composition to an eye of the individual. In some embodiments, the composition comprises aceclidine and has a pH of about 4.5 to about 5.5, optionally of about 5 0 to about 5.5 In some embodiments, the composition has been stored at room temperature for up to 14 days (e.g., up to 3 months) prior to administration of the ophthalmic composition to the eye. In some embodiments, the ophthalmic composition remains ophthalmically acceptable and suitable for treating presbyopia when administered to the eye for at least 14 days (e.g., at least 3 months) at room temperature.

[0028] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmic composition (such as described herein) to an eye of the individual. In some embodiments, the composition comprises aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, the composition has been stored at 2 degrees Celsius to 8 degrees Celsius up to 12-18 months before being stored at room temperature and having been stored at room temperature for up to 6 months prior to administration. In some embodiments, the ophthalmic composition remains ophthalmically acceptable and suitable for treating presbyopia when administered to the eye for at least 12 months at 2 degrees Celsius to 8 degrees Celsius and 6 months at room temperature.

[0029] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmic composition (such as described herein) to an eye of the individual. In some embodiments, the composition comprises aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, the composition has been stored at 2 degrees Celsius to 8 degrees Celsius up to 12-18 months before being stored at room temperature and having been stored at room temperature for up to 14 days (e.g., at least 3 months) prior to administration. In some embodiments, the ophthalmic composition remains ophthalmically acceptable and suitable for treating presbyopia when administered to the eye for at least 12 months at 2 degrees Celsius to 8 degrees Celsius and 14 days (e.g., at least 3 months) at room temperature.

[0030] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmic composition (such as described herein) to an eye of the individual. In some embodiments, the composition comprises aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, the composition has been stored at greater than 25 degrees Celsius and up to 40 degrees Celsius for up to 8 days prior to administration. In some embodiments, the ophthalmic composition remainsophthalmically acceptable and suitable for treating presbyopia when administered to the eye for at least 8 days at 40 degrees Celsius. In some instances, the ability to store a composition for a few days of the 40 degrees Celsius can provide a substantial commercial benefit, such as allowing the composition to be shipped using lower cost and / or more conventional shipping methods, including shipping to pharmacies or directly to end-users.

[0031] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising storing an ophthalmic composition (such as described herein) at a temperature of about 2 °C to about 8 °C. In some embodiments, the ophthalmic composition comprises aceclidine and having a pH of 4.5 to 5.5, optionally of about 5.0 to about 5.5. In some embodiments, prior to storing at a temperature of about 2 °C to about 8 °C, the ophthalmic composition comprises an initial concentration of the aceclidine, wherein the initial concentration of aceclidine in the ophthalmic composition is about 1.35 wt. % to about 1.75 wt. %, based on the free base concentration of aceclidine in the ophthalmic composition. In some embodiments, the method comprises after storing the ophthalmic composition at a temperature of about 2 °C to about 8 °C, storing the ophthalmic composition at room temperature. In some embodiments, the ophthalmic composition comprising at least 90% of the initial concentration of aceclidine for at least 12 months at 2 degrees Celsius to 8 degrees Celsius and 14 days (e.g., at least 3 months) at room temperature. In some embodiments, the method comprises administering the ophthalmic composition to an eye of the individual.

[0032] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising storing an ophthalmic composition (such as described herein) at a temperature of about 2 °C to about 8 °C. In some embodiments, the ophthalmic composition comprising aceclidine and having a pH of 4.5 to 5.5, optionally of about 5.0 to about 5.5. In some embodiments, the method comprises after storing the ophthalmic composition at a temperature of about 2 °C to about 8 °C, storing the ophthalmic composition at room temperature for up to 14 days (e.g., 3 months). In some embodiments, the method comprises administering the ophthalmic composition to an eye of the individual, wherein prior to storing at a temperature of about 2 °C to about 8 °C, the ophthalmic composition comprises an initial concentration of the aceclidine, the initial concentration of aceclidine in the ophthalmic composition being about 1.35 wt. % to about 1.75 wt. %, based on the free base concentration of aceclidine in the ophthalmic composition. In some embodiments, the ophthalmic composition comprising at least 90% of the initial concentration of aceclidine for at least 12 months at 2 degrees Celsius to 8 degrees Celsius and 14 days (e.g., at least 3 months) at room temperature. In some embodiments, the ophthalmic composition is preservative-free (free of antimicrobial preservative). In some embodiments, the ophthalmic composition comprises the aceclidine in solution.

[0033] Provided in some embodiments herein is a system comprising a single-patient-use container (such as described herein) and an ophthalmic composition (such as described herein). In some embodiments, the ophthalmic composition comprises aceclidine or a salt thereof In some embodiments, the single-patient-use container comprises an enclosed chamber, the ophthalmic composition being configured within the enclosed chamber. In some embodiments, the singlepatient-use container is configured to be irreversibly opened, and single-patient-use container being configured, when open, to dispense a first drop and a second drop of the ophthalmic composition through an opening (e.g., formed when the container is irreversibly opened).

[0034] Provided in some embodiments herein is a method for treating presbyopia in an individual, the method comprising opening a first single-patient-use container (such as described herein) on a first day. In some embodiments, the method comprises subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmic composition (such as described herein) to the first eye of the individual. In some embodiments, the second drop is administered to the first eye 2 minutes after the first drop is administered to the first eye. In some embodiments, the ophthalmic composition comprises aceclidine or a salt thereof.

[0035] Provided in some embodiments herein is a method for improving distance vision in an individual, the method comprising opening a first single-patient-use container (such as described herein) on a first day and subsequently administering to a first eye of the individual a first drop of an ophthalmic composition (such as described herein) to the first eye of the individual. In some embodiments, the method comprises subsequently administering to the first eye of the individual a second drop of an ophthalmic composition to the first eye of the individual. In some embodiments, the second drop is administered 2 minutes after the first drop is administered to the first eye. In some embodiments, the ophthalmic composition comprises aceclidine or a salt thereof.

[0036] Provided in some embodiments herein is a system comprising a single-use container (such as described herein) and about 0.5 mb of a (e.g., sterile) ophthalmic composition (such as described herein). In some embodiments, the ophthalmic composition comprises aceclidine or a salt thereof in a concentration of about 1 wt. % to about 2 wt. %. In some embodiments, the singleuse container comprises an enclosed chamber, the ophthalmic composition being configured within the enclosed chamber. In some embodiments, the single-use container is configured to be irreversibly opened, and single-use container being configured, when open, to dispense a first drop and a second drop of the ophthalmic composition through an opening (e.g., formed when the container is irreversibly opened). In some embodiments, the ophthalmic composition does not include an antimicrobial preservative.

[0037] Provided in some embodiments herein is a method of treating presbyopia in an individual wearing a contact lens, the method comprising administering one or more drop of an ophthalmiccomposition comprising aceclidine to an eye of the individual. In some embodiments, the method comprises removing the contact lens from the eye before administering the ophthalmic composition to the eye. In some embodiments, the method comprises reinserting the contact lens onto the eye about 10 minutes or more after administering the ophthalmic composition.

[0038] Provided in some embodiments herein is a method of reducing a corrective lens wear time in an individual, the method comprising administering a composition comprising a miotic (such as described herein) to an eye of the individual. In some embodiments, the miotic is pupil selective (e.g., such as relative to ciliary muscle).

[0039] Provided in some embodiments herein is a method of treating presbyopia in an individual wearing a contact lens, the method comprising administering to an eye of the individual a first drop and a second drop of a (e.g., sterile) ophthalmic composition (such as described herein). In some embodiments, the second drop is administered to the eye about 2 minutes after the first drop. In some embodiments, the (e.g., sterile, preservative-free) ophthalmic composition comprises aceclidine in a concentration of about 1 wt. % to about 2 wt. %. In some embodiments, the method comprises removing the contact lens from the eye before administering the first drop of the ophthalmic composition to the eye. In some embodiments, the method comprises reinserting the contact lens onto the eye about 10 minutes or more after administering the second drop of the ophthalmic composition (such as described herein) to the eye. In some embodiments, the ophthalmic composition does not include an antimicrobial preservative.

[0040] Provided in some embodiments herein is a method of increasing comfortable screen time in an individual, the method comprising administering an ophthalmic composition comprising a miotic (such as described herein) to an eye of the individual. In some embodiments, the ophthalmic composition is a pupil selective miotic composition, relative to ciliary muscle. In some embodiments, the individual has presbyopia. In some embodiments, the individual has myopia.

[0041] In some embodiments, a method provided herein comprises (e.g., topically) administering a first drop of an ophthalmological composition to an eye (e g., an ocular surface thereof) of an individual and subsequently administering a second drop of the ophthalmological composition to the eye (e.g., an ocular surface thereof) of the individual. In some embodiments, a composition provided herein comprises a composition comprising a miotic (e.g., aceclidine or a salt thereof). In some embodiments, the first and second drop are administered about 2 minutes apart.

[0042] In certain instances, providing a first drop and a second drop of a composition provided herein to an eye of an individual provides improved efficacy (e.g., duration) of an effect provided by the composition, such as increased duration of an improvement in visual acuity at 40 cm (e g., relative to administration of a single drop of the composition). In some instances, such an enhanced effect can be provided when the drops are administered as little 5 minutes apart, or less.In certain instances, such an enhanced effect can be unexpectedly provided when the drops are administered as little as about 2 minutes apart, or less. In some instances, such benefits provided when the drops are administered so close together is advantageous because it can improve patient compliance with the protocol. For example, in some instances, patients that have to wait a longer time between drops may become distracted or bored and forget or otherwise fail to administer a second drop, resulting in sub-optimal results.

[0043] In some embodiments, provided herein is a method of treating presbyopia in an individual (e.g., in need thereof), the method comprising (e.g., topically) administering a first drop of an ophthalmological composition to an eye of the individual and subsequently administering a second drop of the ophthalmological composition to the eye of the individual. In specific embodiments, the ophthalmological composition comprises aceclidine or a salt thereof. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0044] In certain embodiments, provided herein is a method of treating presbyopia in an individual (e.g., in need thereof), the method consisting essentially of (e.g., topically) administering a first drop of an ophthalmological composition to an eye of the individual and subsequently administering a second drop of the ophthalmological composition to the eye of the individual. In specific embodiments, the ophthalmological composition comprises aceclidine or a salt thereof. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0045] In certain embodiments, provided herein is a method of treating presbyopia in an individual (e.g., in need thereof), the method comprising (e.g., topically) administering a first drop of an ophthalmological composition to an eye of the individual and subsequently administering a second drop of the ophthalmological composition to the eye of the individual. In specific embodiments, the ophthalmological composition comprises aceclidine or a salt thereof. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0046] Provided in certain embodiments herein, is a method for treating presbyopia and improving distance vision in an individual (e.g., in need thereof), the method comprising opening a first single-patient-use container on a first day and subsequently administering to a first eye ofthe individual a first drop and a second drop of an ophthalmological composition provided herein (e.g., comprising a miotic, such as aceclidine or a salt thereof) to the first eye of the individual. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0047] In some embodiments, the ophthalmological composition comprises aceclidine or a salt thereof in a concentration of about 1 wt. % to about 2 wt. %. In specific embodiments, the ophthalmological composition comprises aceclidine or a salt thereof in a concentration of about 1.75 wt. % (e g., as determined based on the concentration of the aceclidine salt used in the formulation).

[0048] In some embodiments, the ophthalmological composition comprises aceclidine hydrochloride in a concentration of about 1.75 wt. %. In some embodiments, the ophthalmological composition comprises aceclidine in a concentration of about 1.44 wt. % based on the free base concentration of aceclidine.

[0049] In certain embodiments, following administration of the ophthalmological composition to the eye, the individual has 3-lines or more improvement in the eye (e g., at 40 cm, such as determined using BCDVA). In some embodiments, following administration of the ophthalmological composition to the eye, the individual has 3-lines or more improvement in the eye within 0.5 hours of administering the second drop to the eye. In certain embodiments, following administration of the ophthalmological composition to the eye, the individual has 3- lines or more improvement for at least 8 hours in the eye. In specific embodiments, following administration of the ophthalmological composition to the eye, the individual has 3-lines or more improvement for at least 10 hours in the eye. In some embodiments, the method (and / or near vision improvement) is achieved without loss of 1 line or more (e.g., at 4 m).

[0050] In some embodiments, provided herein is a method of providing a pupil size of an eye of an individual to less than 2 millimeters (mm) in the eye of the individual for at least 9 hours, the method comprising administering an ophthalmological composition comprising a miotic agent (e.g., aceclidine) to an eye of the individual.

[0051] In some embodiments, a method provided herein comprises an individual administering (e.g., instilling) the first drop of a composition provided herein in each eye followed by the second drop in each eye two minutes later.

[0052] In certain embodiments, provided herein is a method for treating presbyopia, the method comprising instill one drop of an ophthalmological composition comprises aceclidine or a salt thereof in each eye followed by a second drop in each eye. In specific embodiments, the seconddrop is administered in each eye about two (2) minutes later (after the first drop in each eye). In some embodiments, a method provided herein provides an effect for up to at least 10 hours (e g., an improvement in near vision, distance vision, and / or visual acuity, as disclosed herein).

[0053] In some embodiments, provided herein is a method wherein a second ophthalmological composition is used in (administered to) the eye. In certain embodiments, the ophthalmological composition or the second ophthalmological composition is administered at least 5 minutes after the last dose of the other of the ophthalmological composition or the second ophthalmological composition, which was administered first.

[0054] In some embodiments, an ophthalmological composition provided herein does not comprise a cycloplegic agent. In some embodiments, an ophthalmological composition provided herein does not comprise tropicamide.

[0055] In some embodiments, an ophthalmological composition provided herein is (substantially) preservative-free (e.g., less than 0.2 wt. %, less than 0.1 wt. %, or 0 wt. %). In specific embodiments, the composition does not comprise a preservative.

[0056] In certain embodiments, no more than a first drop and a second drop of the ophthalmological composition is administered to an eye (e.g., in a day).

[0057] In some embodiments, an ophthalmological composition provided herein is administered to the eye (e.g., two drops, such as once daily) for at least 6 weeks. In some embodiments, an ophthalmological composition provided herein is administered to the eye (e g., two drops, such as once daily) for at least 24 weeks.

[0058] In certain embodiments, an individual receiving the drops according to a method provided herein wears a contact lens, such as in the eye to which the drop(s) are administered. In some embodiments, the individual removes the contact lens before administering the ophthalmological composition to the eye. In certain embodiments, the individual inserts or reinserts the contact lens onto the eye at least about 10 minutes (e g., at least about 15 minutes) after administering the second drop of the ophthalmological composition.

[0059] In certain embodiments, an ophthalmological composition provided herein is a pupil selective miotic composition.

[0060] In some embodiments, a composition provided herein comprises a miotic that is a pupil selective miotic. In specific embodiments, the pupil selective miotic is aceclidine or an ophthalmically acceptable salt thereof.

[0061] In some embodiments, provided herein is a method of reducing a corrective lens (e g., bifocal or contact lens) wear time in an individual, the method comprising administering a composition comprising a miotic to an eye of the individual. In some embodiments, provided herein is a method of reducing a corrective lens (e.g., corrective glasses, such as bifocal, or contactlens) wear time in an individual, the method comprising administering a composition comprising a miotic to an eye of the individual.

[0062] In some embodiments, a lens is a bifocal glasses, progressive glasses, or a contact lens

[0063] In certain embodiments, an individual treated according to a method provided herein is less dependent on wearing bifocals (e.g., glasses, progressive glasses, reading glasses), and / or contacts, such as during the workday. In certain embodiments, an individual treated according to a method provided herein is less dependent on wearing correctives glasses (e.g., bifocal glasses, progressive glasses, reading glasses), and / or contacts, such as during the workday.

[0064] In certain embodiments, an individual treated according to a method provided herein is, following treatment, less dependent on or no longer requires wearing a bifocal glasses, progressive glasses, reading glasses, or a contact lens to correct near vision. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +0.75. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +1.5. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +2.0. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +2.5. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +3.0. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +3.5.

[0065] In certain embodiments, an individual treated according to a method provided herein has moderate presbyopia. In certain embodiments, an individual treated according to a method provided herein has advanced presbyopia.

[0066] In certain embodiments, an individual treated according to a method provided herein has an improvement in near vision (e.g., at 40 cm) of at least 4 lines. In certain embodiments, the individual has an improvement in near vision (e.g., at 40 cm) of at least 5 lines. In certain embodiments, the individual has an improvement in near vision (e.g., at 40 cm) of at least 6 lines.

[0067] In certain embodiments, an individual treated according to a method provided herein has an improvement in distance vision (e.g., at 4 m) of at least 1 line.

[0068] In certain embodiments, an individual treated according to a method provided herein has an improvement in distance vision (e.g., at 4 m) of at least 3 letters.

[0069] In certain embodiments, an individual treated according to a method provided herein is at least 60 years old. In certain embodiments, the individual is 61-65 years old. In certain embodiments, the individual is 66-70 years old.

[0070] Provided in some embodiments herein are aceclidine compositions, such as aqueous aceclidine compositions having a pH of about 6 or less (e.g., about 4.5 or about 5.5), that have acceptable aceclidine stability profiles at room temperature (or higher temperatures) for extended periods of time. Additionally, in some embodiments, compositions described herein, such as aqueous aceclidine compositions described herein, have aceclidine stability profiles that are acceptable for manufacture, shipment, and / or (e g., long-term and end-user) storage of aceclidine at room temperature (e.g., up to 25 degrees Celsius) (or higher temperatures). In certain instances, targeted pH of aceclidine compositions provided herein allowed for long term room temperature storage of compositions described herein, irrespective of whether or not other components were varied. For example, figures and examples provided herein demonstrate substantially similar storage stability for compositions, irrespective of presence or lack thereof of a preservative and / or irrespective of buffer concentration. In some instances, aceclidine stability of aceclidine compositions described herein, surprisingly, did not improve by changing certain parameters of the compositions, such as addition of a preservative and different buffer concentrations.

[0071] Provided herein are (e.g., aqueous) compositions that are stable at room temperature, retaining high (purity) levels (e.g., 90% or more, 95% or more, or even 98% or more) of aceclidine in the composition (e.g., compared to a baseline or an initial amount of aceclidine in the composition) at room temperature for extended periods of time, such as for up to 3 months. In some embodiments, provided herein are methods involving storing of a composition of up to 3 months or 6 months or the like. In certain embodiments, storage of a composition of up to x months, indicates that the composition is suitable for storage under the indicated conditions for any time period up to x months (i.e., inclusive of all time points up to x months in the absence of use or disposal of the composition). In other words, storage of a composition for “up to” x months means that the composition is suitable for use for the entire duration of x months. For example, in some embodiments, when a composition is stored for up to x months according to a composition or method provided herein, the composition is suitable for subsequent use (e.g., having at least 90% initial aceclidine) at any point up to x months. In some instances, compositions described herein having a pH of about 6 or less, or less than 6 (e.g., a pH of about 4.5 to about 5.5 or about 5.0 to about 5.5) have substantially better aceclidine stability profiles than compositions described herein having a pH of greater than 6, or 6 or more. In some instances, a delineation exists between the stability of aceclidine compositions described herein having a pH of 6 or less (e.g., pH of about 4.5 to about 5.5 or about 5.0 to about 5.5) and equivalent compositions having a pH of 6 or more. In some instances, even changing other aceclidine composition parameters, such as buffer concentration or adding a preservative, has little or no effect on the stability of aceclidine in the compositions described herein, such as compositions having a pH of less than 6.

[0072] In some embodiments, compositions provided herein retain high (purity) levels (e.g., 90% or more, 95% or more, or even 98% or more) of aceclidine in the composition (e.g., compared to a baseline or an initial amount of aceclidine in the composition) at even higher temperatures (e g., 40 degrees Celsius (°C)) for extended periods of time, such as for up to 2 months. In some embodiments, compositions provided herein retain high (purity) levels (e.g., 90% or more, 95% or more, or even 98% or more) of aceclidine in the composition (e g , compared to a baseline or an initial amount of aceclidine in the composition) during manufacture, shipment, and / or storage of the composition, such as at temperatures ranging from 0 °C to room temperature, or higher temperatures.

[0073] In some embodiments, a composition provided herein comprises aceclidine or a salt thereof and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, a composition provided herein comprises aceclidine or a salt thereof, a viscosity agent, and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, a composition provided herein comprises aceclidine or a salt thereof, a (nonionic) surfactant, and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, a composition provided herein comprises aceclidine or a salt thereof, a (nonionic) surfactant, a viscosity agent, and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5.

[0074] In some embodiments, a composition described herein has a room temperature when administered to the eye of an individual described herein. In some embodiments, a composition described herein has been stored at room temperature for up to 6 months prior to administration to an individual described herein. In some embodiments, a composition described herein has been stored up to 18 months before being stored at room temperature for up to 6 months prior to administration to an individual described herein.

[0075] In certain embodiments, provided herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmologically acceptable composition to an eye of the individual. In specific embodiments, the composition comprises aceclidine and has a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, the composition has a room temperature (e.g., up to 25 degrees Celsius) when administered to the eye of the individual. In specific embodiments, a composition has a room temperature above 8 degrees Celsius and up to 25 degrees Celsius.

[0076] In some embodiments, provided herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmologically acceptable composition to an eye of the individual. In certain embodiments, the composition comprises aceclidine and has a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In specific embodiments, thecomposition having been stored at room temperature (e.g., up to 25 degrees Celsius). In specific embodiments, the composition has been stored at room temperature for up to 3 months prior to administration In some embodiments, the composition has been stored for up to 6 months prior to administration. In some embodiments, the composition has been stored at room temperature for 1 month prior to administration. In specific embodiments, the composition has been stored at room temperature for 2 months prior to administration In specific embodiments, the composition has been stored at room temperature for 3 months prior to administration. In specific embodiments, a composition has a room temperature above 8 degrees Celsius and up to 25 degrees Celsius.

[0077] In certain embodiments, provided herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmologically acceptable composition to an eye of the individual. In some embodiments, the composition comprises aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In certain embodiments, the composition has been stored at 2 degrees Celsius to 8 degrees Celsius up to 12-18 months (e g., 6-18 month, or 12-18 months). In specific embodiments, the composition has been stored at 2 degrees Celsius to 8 degrees Celsius before being stored at room temperature. In specific embodiments, the composition has been stored at room temperature for up to 3 months prior to administration. In some embodiments, the composition has been stored for up to 6 months prior to administration. In some embodiments, the composition has been stored at room temperature for 1 month prior to administration. In specific embodiments, the composition has been stored at room temperature for 2 months prior to administration. In specific embodiments, the composition has been stored at room temperature for 3 months prior to administration. In specific embodiments, a composition has a room temperature above 8 degrees Celsius and up to 25 degrees Celsius.

[0078] In certain embodiments, provided herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmologically acceptable composition to an eye of the individual. In specific embodiments, the composition comprises aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, the composition has been stored at greater than 25 degrees Celsius and up to 40 degrees Celsius for up to 8 days (e.g., 1-8 days) prior to administration.

[0079] In certain embodiments, a composition provided herein has a pH of about 4.5 to about 5. In some embodiments, a composition provided herein has a pH of about 5 to about 5.5.

[0080] In some embodiments, the composition has an initial amount of aceclidine (e.g., 100%), and wherein at least 90% of the initial amount of aceclidine is present in the composition when administering the composition to the eye of the individual (e g., after the storage protocols provided herein).

[0081] In some embodiments, prior to administration, the composition has been stored for up to 6 months. In certain embodiments, prior to administration, the composition has been stored at a temperature of about 0 degrees Celsius to about 10 degrees Celsius. In specific embodiments, prior to administration, the composition has been stored at a temperature of about 2 degrees Celsius to about 8 degrees Celsius.

[0082] In some embodiments, prior to administration, the composition has been stored at a temperature up to about 40 degrees Celsius.

[0083] In certain embodiments, a composition provided herein comprises an aqueous medium.

[0084] In certain embodiments, a composition provided herein comprises a viscosity agent. In specific embodiments, the composition comprises a concentration of the viscosity agent of about 0.5 wt. % to about 5 wt. %. In more specific embodiments, the composition comprises a concentration of the viscosity agent of about 1 wt. % to about 1.5 wt. %.

[0085] In certain embodiments, a composition provided herein comprises a nonionic surfactant. In some embodiments, the composition comprises a concentration of the nonionic surfactant of about 2 wt. % to about 10 wt. %. In specific embodiments, the composition comprises a concentration of the nonionic surfactant of about 3 wt. % to about 5 wt. %.

[0086] In certain embodiments, a composition provided herein comprises a concentration of the aceclidine of about 0.2 wt. % to about 4 wt. %.

[0087] In certain embodiments, a composition provided herein comprises a concentration of the buffer of about 0.06 wt. % to about 0.1 wt. %.

[0088] In certain embodiments, a composition provided herein does not comprise a preservative.

[0089] In certain embodiments, a composition provided herein has been stored at a temperature from about 0 degrees Celsius to about 10 degrees Celsius, such as for up to 24 months. In certain embodiments, a composition provided herein has been stored at a temperature from about 2 degrees Celsius to about 8 degrees Celsius, such as for up to 24 months.

[0090] In certain embodiments, a composition provided herein has been stored at a temperature from about 0 degrees Celsius to about 10 degrees Celsius, such as for up to 12-18 months. In certain embodiments, a composition provided herein has been stored at a temperature from about 2 degrees Celsius to about 8 degrees Celsius, such as for up to 12-18 months.

[0091] In certain embodiments, a composition provided herein has been stored up to 24 months (e.g., at 2 degrees Celsius to 8 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 90 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 24 months (e.g., at 2 degrees Celsius to 8 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 95 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior tosuch storage) of aceclidine after storage for 24 months (e.g., at 2 degrees Celsius to 8 degrees Celsius).

[0092] In certain embodiments, a composition provided herein has been stored up to 18 months (e.g., at 2 degrees Celsius to 8 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 90 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 18 months (e g., at 2 degrees Celsius to 8 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 95 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 18 months (e g., at 2 degrees Celsius to 8 degrees Celsius).

[0093] In certain embodiments, a composition provided herein has been stored up to 12 months (e.g., at 2 degrees Celsius to 8 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 90 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 12 months (e.g., at 2 degrees Celsius to 8 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 95 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 12 months (e.g., at 2 degrees Celsius to 8 degrees Celsius).

[0094] In certain embodiments, a composition provided herein has been stored up to 6 months (e.g., at room temperature, such as up to 25 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 90 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 6 months (e g., at room temperature, such as up to 25 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 95 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 6 months (e.g., at room temperature, such as up to 25 degrees Celsius).

[0095] In certain embodiments, a composition provided herein has been stored up to 3 months (e.g., at room temperature, such as up to 25 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 90 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 3 months (e.g., at room temperature, such as up to 25 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 95 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 3 months (e.g., at room temperature, such as up to 25 degrees Celsius).

[0096] In certain embodiments, a composition provided herein has been stored up to 2 weeks (e.g., at room temperature, such as up to 25 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 90 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 2 weeks (e.g., at room temperature, such as up to 25 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 95 wt. % of the initial amount (e g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 2 weeks (e.g., at room temperature, such as up to 25 degrees Celsius).

[0097] Provided in some embodiments herein is a method of storing an ophthalmologically acceptable composition, the method comprising storing the composition for up to 6 months at room temperature, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5, wherein following the up to 6 months (e.g., 2-6 months) of storage at room temperature, the composition comprises at least 90% of the amount of aceclidine as prior to the up to 6 months (e.g., 1-6 months) of storage at room temperature.

[0098] Provided in some embodiments herein is a method of storing an ophthalmologically acceptable composition, the method comprising storing the composition for up to 12-18 months followed by storing the composition for up to 6 months (e.g., 2-6 months) at room temperature, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5, wherein following the up to 6 months of storage at room temperature, the composition comprises at least 90% of the amount of aceclidine as prior to the up to 6 months (e.g., 2-6 months) of storage at room temperature.

[0099] Provided in some embodiments herein is a method of storing an ophthalmologically acceptable composition, the method comprising storing the composition for up to 6 months at room temperature, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5, wherein following the up to 3 months (e.g., 2-3 months) of storage at room temperature, the composition comprises at least 90% of the amount of aceclidine as prior to the up to 3 months (e.g., 2-3 months) of storage at room temperature.

[0100] Provided in some embodiments herein is a method of storing an ophthalmologically acceptable composition, the method comprising storing the composition for up to 12-18 months followed by storing the composition for up to 3 months (e.g., 2-3 months) at room temperature, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5, wherein following the up to 6 months of storage at room temperature, the composition comprises at least 90% of the amount of aceclidine as prior to the up to 3 months (e.g., 2-3 months) of storage at room temperature.

[0101] In some embodiments, a method provided herein comprises storing the composition at a temperature from about 0 degrees Celsius to about 10 degrees Celsius (e.g., 2-8C), such as for up to 12-18 months (e g., for 12-18 months). In some embodiments, a method provided herein comprises storing the composition for up to 18 months (e.g., 12-18 months).

[0102] In some embodiments, a method provided herein comprises storing the composition for up to 12 months (e.g., 6-12 months).

[0103] In some embodiments, a method provided herein comprises storing the composition for up to 6 months (e.g., 3-6 months).

[0104] Also provided herein is an ophthalmological composition comprising a general miotic agent for use in a method described herein of providing a pupil eye size of an individual to less than 2 millimeters (mm) in the eye of the individual for at least 9 hours. Also provided herein is a miotic for use in a method described herein of reducing corrective lens wear time in an individual. Also provided herein is a miotic for use in a method described herein of increasing comfortable screen time in an individual. In some cases, the miotic is pupil selective, relative to the ciliary muscle. Also provided herein is an ophthalmological composition for use in a method described herein of treating presbyopia in an individual. In some cases, the ophthalmological composition comprises a miotic. In some cases, the miotic is pupil selective, relative to the ciliary muscle. In some cases, the ophthalmological composition comprises aceclidine or a salt thereof. The methods may comprise any step as described herein.

[0105] Provided in certain embodiments herein is a composition for use in treating (e.g., in a method of treating) presbyopia in an individual, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5, and the composition having a room temperature when administered to an eye of the individual.

[0106] Provided in certain embodiments herein is a composition for use in treating (e.g., in a method of treating) presbyopia in an individual, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5, and having been stored at room temperature for up to 6 months prior to administering to an eye of the individual.

[0107] Provided in certain embodiments herein is a composition for use in treating (e.g., in a method of treating) presbyopia in an individual, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5, and having been stored up to 12-18 months before being stored at room temperature, and having been stored at room temperature for up to 6 months prior to administering to an eye of the individual.

[0108] Provided in certain embodiments herein is a composition for use in treating (e.g., in a method of treating) presbyopia in an individual, the composition comprising aceclidine and a pHof about 4.5 to about 5.5, optionally of about 5.0 to about 5.5, and having been stored at room temperature for up to 3 months (e.g., 2-3 months) prior to administering to an eye of the individual.

[0109] Provided in certain embodiments herein is a composition for use in treating (e g , in a method of treating) presbyopia in an individual, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5, and having been stored up to 12-18 months (e g., for 12-18 months) before being stored at room temperature, and having been stored at room temperature for up to 3 months (e g., 2-3 months) prior to administering to an eye of the individual.[HO] In some embodiments, the individual is or has been selected from a group of individuals who have previously had ocular surgery in a first eye, a second eye, or both eyes. In some embodiments the ocular surgery is laser assisted in situ keratomileusis (LASIK) surgery or photorefractive keratectomy (PRK) surgery. In some embodiments, the individual is 61-65 years old. In some embodiments, the individual is 66-70 years old. In some embodiments, the individual is selected from a group of individuals who wear contact lenses.[Hl] In some embodiments, the ophthalmological composition comprises aceclidine or a salt thereof in a concentration of about 1 wt. % to about 2 wt. %, optionally about 1.2 wt. % to about 1.8 wt. %. In some embodiments, the ophthalmological composition comprises aceclidine in a free base concentration of about 1.35 wt.% to about 1.75 wt. %, optionally about 1.44 wt. %. In some cases, the ophthalmological composition comprises about 1.7 wt. % to about 1.8 wt. % aceclidine hydrochloride. In some embodiments, the ophthalmological composition is preservative-free. The ophthalmological composition may comprise any component as described herein. One or more ophthalmological compositions may be administered to the eye. The one or more ophthalmological compositions may the same or different ophthalmological compositions. In some cases, no more than a first drop and a second drop of the ophthalmological composition is administered to the eye in a day.

[0112] In some cases, the ophthalmological composition has a room temperature when administered to the eye of the individual. Prior to administration, the ophthalmological composition may have been stored in any way described herein.

[0113] In some cases, the composition provides 3 -lines or more improvement in the eye of the individual within 0.5 hours of administration to the eye. In some cases, the composition provides 3 -lines or more improvement in the eye of the individual for at least 8 hours following administration to the eye.

[0114] In some embodiments, provided herein is a composition having a pH of about 4.5 to about 5. In some embodiments, provided herein is a composition having a pH of about 5 to about 5.5.[H5] In some embodiments, provided herein is a composition that has an initial amount of aceclidine (e.g., 100%), and wherein at least 90% of the initial amount of aceclidine being present in the composition upon administration of the composition to the eye of the individual.

[0116] In some embodiments, prior to administration, a composition provided herein has been stored for up to 6 months. In some embodiments, prior to administration, a composition provided herein has been stored for up to 3 months (e g., 2-3 months), such as at room temperature.

[0117] In some embodiments, prior to administration, a composition provided herein has been stored at a temperature of about 0 degrees Celsius to about 10 degrees Celsius. In some embodiments, prior to administration, a composition provided herein has been stored at a temperature of about 2 degrees Celsius to about 8 degrees Celsius. In specific embodiments, the composition has been stored at such a temperature for up to 18 months (e.g., for 12-18 months).

[0118] In some embodiments, prior to administration, a composition provided herein has been stored at a temperature up to about 40 degrees Celsius.

[0119] In some embodiments, a composition provided herein comprises an aqueous medium.

[0120] In some embodiments, a composition provided herein comprises a viscosity agent. In specific embodiments, the composition comprises a concentration of the viscosity agent of about 0.5 wt. % to about 5 wt. %. In more specific embodiments, the composition comprises a concentration of the viscosity agent of about 1 wt. % to about 1.5 wt. %.

[0121] In some embodiments, a composition provided herein comprises a nonionic surfactant. In specific embodiments, the composition comprises a concentration of the nonionic surfactant of about 2 wt. % to about 10 wt. %. In still more specific embodiments, the composition comprises a concentration of the nonionic surfactant of about 3 wt. % to about 5 wt. %.

[0122] In some embodiments, a composition provided herein comprises a concentration of the aceclidine of about 0.2 wt. % to about 4 wt. %.

[0123] In some embodiments, a composition provided herein comprises a concentration of the buffer of about 0.06 wt. % to about 0.1 wt. %.

[0124] In some embodiments, a composition provided herein does not comprise a preservative.

[0125] In some embodiments, a composition provided herein has been stored at a temperature of about 0 degrees Celsius to about 10 degrees Celsius for up to 12-18 months. In some embodiments, a composition provided herein has been stored up to 18 months (e.g., for 12-18 months). In some embodiments, the composition has been stored up to 12 months. In certain embodiments, the composition has been stored up to 6 months.

[0126] Provided in certain embodiments herein is a method of treating presbyopia in an individual, the method comprising administering the ophthalmologically acceptable composition to an eye of the individual, the composition comprising: aceclidine or a salt thereof at aconcentration of about 1 weight percent (wt. %) to about 2 wt. %; and a pH of 4.5 to 5.5, optionally of about 5.0 to about 5.5, the composition having been stored at room temperature, up to 25 degrees Celsius, for up to 3 months (e g., for 2-3 months) prior to administration.

[0127] Provided in some embodiments therein is a method of treating presbyopia in an individual, the method comprising (i) storing an ophthalmologically acceptable composition, and (ii) administering the ophthalmologically acceptable composition to an eye of the individual, the composition comprising: aceclidine or a salt thereof at a concentration of about 1 weight percent (wt. %) to about 2 wt. %; and a pH of 4.5 to 5.5, optionally of about 5.0 to about 5.5, the composition having been stored at room temperature, up to 25 degrees Celsius, for up to 3 months (e.g., 2-3 months) prior to administration.

[0128] In some embodiments, the composition has been stored at room temperature for up to 6 months prior to administration.

[0129] In certain embodiments, the composition has been stored at room temperature for 1 month prior to administration. In some embodiments, the composition has been stored at room temperature for 2 months prior to administration. In some embodiments, the composition has been stored at room temperature for 3 months prior to administration.

[0130] In some embodiments, a composition provided herein has a pH of about 5 to 5.5.

[0131] In certain embodiments, a composition provided herein has an initial amount of aceclidine and at least 90% of the initial amount of aceclidine is present in the composition when the composition is administered to the eye of the individual.

[0132] In some embodiments, prior to administration, a composition provided herein has been stored at a temperature of 2 degrees Celsius to 8 degrees Celsius. In certain embodiments, prior to administration, the composition has been stored at a temperature of 2 degrees Celsius to 8 degrees Celsius for 12 months. In certain embodiments, prior to administration, the composition has been stored at a temperature of 2 degrees Celsius to 8 degrees Celsius for 18 months.

[0133] In certain embodiments, prior to administration, the composition has been stored at a temperature of greater than 25 degrees Celsius and up to 40 degrees Celsius. In certain embodiments, prior to administration, the composition has been stored at a temperature of greater than 25 degrees Celsius and up to 40 degrees Celsius for up to 8 days (e.g., for 1-8 days).

[0134] In some embodiments, a composition provided herein comprises a viscosity agent.

[0135] In some embodiments, a composition provided herein comprises a nonionic surfactant.

[0136] Provided in certain embodiments herein, is a method for treating presbyopia in an individual (e.g., in need thereof), the method comprising opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition provided herein (e.g., comprising a miotic,such as aceclidine or a salt thereof) to the first eye of the individual. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye. In some embodiments, the ophthalmological composition is as described herein, such as having been stored as described herein.

[0137] Provided in certain embodiments herein, is a method for treating presbyopia and improving distance vision in an individual (e.g., in need thereof), the method comprising opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition provided herein (e.g., comprising a miotic, such as aceclidine or a salt thereof) to the first eye of the individual. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye. In some embodiments, the ophthalmological composition is as described herein, such as having been stored as described herein.

[0138] In certain embodiments, an individual treated according to a method provided herein has moderate presbyopia. In certain embodiments, an individual treated according to a method provided herein has advanced presbyopia.

[0139] In certain embodiments, an individual treated according to a method provided herein has an improvement in near vision (e.g., at 40 cm) of at least 4 lines. In certain embodiments, the individual has an improvement in near vision (e.g., at 40 cm) of at least 5 lines. In certain embodiments, the individual has an improvement in near vision (e.g., at 40 cm) of at least 6 lines.

[0140] In certain embodiments, an individual treated according to a method provided herein has an improvement in distance vision (e.g., at 4 m) of at least 1 line.

[0141] In certain embodiments, an individual treated according to a method provided herein has an improvement in distance vision (e.g., at 4 m) of at least 3 letters.

[0142] In certain embodiments, an individual treated according to a method provided herein is at least 60 years old. In certain embodiments, the individual is 61-65 years old. In certain embodiments, the individual is 66-70 years old

[0143] Provided in certain embodiments herein are systems, compositions, and methods, such as for treating ocular disorders, such as presbyopia. In some embodiments, provided herein is a method of improving vision. In some embodiments, provided herein is a method of improving near vision. In some embodiments, provided herein is a method of improving distance vision. In some embodiments, provided herein is a method of treating presbyopia. In some embodiments, acomposition provided herein comprises a composition comprising a miotic (e.g., aceclidine or a salt thereof).

[0144] In some embodiments, a system provided herein comprises such a composition (e.g., and a vessel containing the composition). In some embodiments, a method provided herein comprises administering such a composition, such as to an eye (e.g., surface) of an individual.

[0145] Provided in some embodiments herein is a method of treating presbyopia in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmological composition to an eye (e g., an ocular surface thereof) of the individual.

[0146] In some embodiments, a method provided herein comprises (e.g., topically) administering a first drop of an ophthalmological composition to an eye (e g., an ocular surface thereof) of an individual and subsequently administering a second drop of the ophthalmological composition to the eye (e.g., an ocular surface thereof) of the individual. In some embodiments, a composition provided herein comprises a composition comprising a miotic (e.g., aceclidine or a salt thereof).

[0147] In certain embodiments, provided herein is a system comprising a single use container and a (e.g., ophthalmological) composition comprising aceclidine or a salt thereof. In some instances, systems provided herein facilitate the use of a dissolved aceclidine that has good stability, including at extended storage times (e.g., including under refrigerated and room temperature storage conditions).

[0148] Aceclidine, which is an approved therapy for glaucoma, provides meaningful therapeutic effects in presbyopic individuals. For example, administering certain aceclidine compositions to the eyes of presbyopic individuals provides beneficial effects, such as improvements to nearsighted vision without affecting baseline distance vision. However, aceclidine is susceptible to degradation, particularly when in solution, so formulating aceclidine in aqueous environments, such as in the form of an eye drop, is challenging. To avoid aceclidine degradation and problems associated with such degradation (e.g., decreased efficacy), aceclidine has typically been stored in a two-bottle system comprising lyophilized aceclidine, which is reconstituted before topical installation to the eye. Certain cold-chain storage protocols have been found to substantially improve the stability profiles of certain aceclidine compositions, such as at temperatures suitable for refrigeration. However, maintaining aceclidine stability at room temperature for prolonged periods of time, such as for more than a few months, has remained a challenge. In addition, requiring end users to store aceclidine compositions at cold temperatures can be a challenge, leading to storage at temperatures not suitable for retaining efficacy for the intended uses of aceclidine products. As such, the instability of aceclidine in aqueous environments poses a challenge for preparing, shipping, and storing aqueous aceclidine compositions.

[0149] Provided in some embodiments herein are compositions and systems, that have acceptable aceclidine stability profiles at room temperature (or higher temperatures) for extended periods of time, even when filled and loaded into a container under non-inert conditions, such as under an oxygen containing gas, e.g., air. Additionally, in some embodiments, compositions described herein and systems provided herein comprising such compositions, such as aqueous aceclidine compositions described herein, have aceclidine stability profiles that are acceptable for manufacture, shipment, and / or (e.g., long-term and end-user) storage of aceclidine at room temperature (e.g., up to 25 degrees Celsius) (or higher temperatures). In certain instances, systems and compositions provided herein allowed for long term room temperature storage of compositions described herein, irrespective of whether or not other components were varied.

[0150] Provided herein are (e.g., aqueous) compositions (e.g., in a system provided herein) that are stable at room temperature, retaining high (purity) levels (e.g., 90% or more, 95% or more, or even 98% or more) of aceclidine in the composition (e.g., compared to a baseline or an initial amount of aceclidine in the composition) at room temperature for extended periods of time, such as for up to 3 months. In some embodiments, provided herein are methods involving storing of a composition for up to 3 months or 6 months or the like. In certain embodiments, storage of a composition for up to x months (e.g., 3 months), indicates that the composition is suitable for storage under the indicated conditions for any time period up to x months (e g., 3 months). For example, in some embodiments, when a composition is stored for up to x months according to a composition or method provided herein, the composition is suitable for subsequent use (e g., having at least 90% initial aceclidine) at any point up to x months.

[0151] Provided herein are (e.g., aqueous) compositions (e.g., in a system provided herein) that are stable (e.g., under specified conditions, such as at room temperature). In some embodiments, stable compositions retain high (purity) levels (e.g., 90% or more, 95% or more, or even 98% or more) of aceclidine in the composition (e.g., compared to a baseline or an initial amount of aceclidine in the composition) (e.g., under specified conditions, such as at room temperature). In some embodiments, composition provided herein are stable (e.g., under specified conditions, such as at room temperature), for extended periods of time, such as for up to 3 months or more. In some embodiments, a stable composition that is stable for up to a specified duration of time (x), the stable composition is stable for any amount of time, up to the specified duration of time, inclusive of the terminal time point. For example, a stable composition that is stable for up to 3 months is stable for 1 week, 1 month, 2 months, and 3 months, provided that it has not already been used prior to the specified time point. In other words, in some instances (e.g., wherein the stability is inclusive of the terminal time point), if a stable composition that is stable for up to 3 months is used after 3 months, it was stable for the entirety of the 3 months. However, if that samecomposition was used at the 2 months, it was stable for the entirety of the 2 months and would have been stable for the entirety of the 3 months, if it had not been used at the 2 months. As used herein, any disclosure of a composition having a stability of up to a specified time point, includes a disclosure of the composition having a stability of up to a specified time point, inclusive of that time point (e.g., assuming that it had not been previously used).

[0152] In some embodiments, compositions provided herein retain high (purity) levels (e g., 90% or more, 95% or more, or even 98% or more) of aceclidine in the composition (e.g., compared to a baseline or an initial amount of aceclidine in the composition) at even higher temperatures (e g., 40 degrees Celsius (°C)) for extended periods of time, such as for up to 2 months In some embodiments, compositions provided herein retain high (purity) levels (e.g., 90% or more, 95% or more, or even 98% or more) of aceclidine in the composition (e.g., compared to a baseline or an initial amount of aceclidine in the composition) during manufacture, shipment, and / or storage of the composition, such as at temperatures ranging from 0 °C to room temperature, or higher temperatures.

[0153] In some embodiments, provided herein is a system comprising a single-patient-use container and an ophthalmological composition. In specific embodiments, the ophthalmological composition comprises aceclidine or a salt thereof. In some embodiments, the single-patient-use container comprises an enclosed chamber. In specific embodiments, the ophthalmological composition is configured within the enclosed chamber of the single patient-use-container. In some embodiments, the single-patient-use container is configured to be irreversibly opened, and single-patient-use container being configured, when open, to dispense a first drop and a second drop of the ophthalmological composition through an opening (e.g., an opening formed upon irreversibly opening of the container).

[0154] In some embodiments, a single-patient-use container provided herein is a vial or an ampoule. In some embodiments, single-patient-use container is a vial (e.g., plastic vial).

[0155] In certain embodiments, the single-patient-use container comprises a container body, a container neck, and a container head, the container body and the container head having a larger cross-sectional dimension than the container neck, the enclosed chamber extending from the container body into the container neck, and the single-patient-use container being configured to be opened at the container neck.

[0156] In some embodiments, a single-patient-use container provided herein (e.g., prior to opening) is sterile. In specific embodiments, the enclosed chamber of the container is sterile.

[0157] In some embodiments, a single-patient-use container provided herein is transparent.

[0158] In certain embodiments, a single-patient-use container provided herein comprises polyethylene. In specific embodiments, the container comprises a low-density polyethylene (LDPE).

[0159] In some embodiments, a system provided herein comprises about 0.2 mL to about 2 mb of the ophthalmological composition. In specific embodiments, the system comprises about 0.2 mL to about 1 mL of the ophthalmological composition. In more specific embodiments, the system comprises about 0.5 mL of the ophthalmological composition.

[0160] In certain embodiments, an ophthalmological composition provided herein comprises aceclidine or a salt thereof in a concentration of about 1 wt. % to about 2 wt %. In specific embodiments, the ophthalmological composition comprises aceclidine or a salt thereof in a concentration of about 1.75 wt. % (e.g., based on the weight of a salt form of aceclidine formulated into the composition).

[0161] In some embodiments, the ophthalmological composition comprises aceclidine hydrochloride in a concentration of about 1.75 wt. %. In some embodiments, the ophthalmological composition comprises aceclidine in a concentration of about 1.44 wt. % based on the free base concentration of aceclidine.

[0162] In some embodiments, a system provided herein comprises a package and at least 3 identical containers and at least 3 identical ophthalmological compositions.

[0163] Provided in certain embodiments herein, is a method for treating presbyopia in an individual (e.g., in need thereof), the method comprising opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition provided herein (e.g., comprising a miotic, such as aceclidine or a salt thereof) to the first eye of the individual. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0164] Provided in certain embodiments herein, is a method for treating presbyopia and improving distance vision in an individual (e.g., in need thereof), the method comprising opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition provided herein (e.g., comprising a miotic, such as aceclidine or a salt thereof) to the first eye of the individual. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0165] In some embodiments, a method provided herein comprises administering to a second eye of the individual a third drop and a fourth drop of an ophthalmological composition to the second eye of the individual In some embodiments, the fourth drop is administered to the eye of the individual about 5 minutes or less after the third drop is administered to the eye. In some embodiments, the fourth drop is administered to the eye of the individual about 2 minutes after the third drop is administered to the eye. In some embodiments, third drop is administered to the second eye prior to the second drop being administered to the first eye.

[0166] In certain embodiments, provided herein is a method comprising opening a second singlepatient-use container on a second day and subsequently administering to the first eye of the individual a second container first drop and a second container second drop of a second container ophthalmological composition. In some embodiments, the second container ophthalmological composition provided herein (e.g., comprising a miotic, such as aceclidine or a salt thereof). In specific embodiments, the first and second days are different days. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0167] In specific embodiments, the ophthalmological composition and the second container ophthalmological composition are identical.

[0168] In some embodiments, any system provided herein comprises a container provided herein, with a composition provided herein and a gas within the chamber thereof. In certain embodiments, the gas within the chamber need not be an inert gas. In some instances, a composition of a system provided herein is stable (e.g., good stability of aceclidine, such as provided herein), including at room temperature, even without the need for an inert gas.

[0169] In some embodiments, a system provided herein comprises a container provided herein, with a composition provided herein and an oxygen containing gas within the chamber thereof. In specific embodiments, the oxygen containing gas is air (e.g., sterile air).

[0170] In certain instances, previously unreported extended effect of two drop doses achievable with a limited amount of time between drops allows for the use a (e.g., sterile and preservative free) composition with a single use container. In some instances, longer delay between drops may require multiple containers for a single dose because long duration between drop administration may be problematic with an aceclidine solution, particularly one that is preservative free.

[0171] In some instances, compositions described herein having a pH of about 6 or less, or less than 6 (e.g., a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5) have substantially better aceclidine stability profiles than compositions described herein having a pH of greater than 6, or 6 or more. In some instances, a delineation exists between the stability of aceclidinecompositions described herein having a pH of 6 or less (e.g., pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5) and equivalent compositions having a pH of 6 or more. In some instances, even changing other aceclidine composition parameters, such as buffer concentration or adding a preservative, has little or no effect on the stability of aceclidine in the compositions described herein, such as compositions having a pH of less than 6.

[0172] In some embodiments, a composition provided herein comprises aceclidine or a salt thereof and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, a composition provided herein comprises aceclidine or a salt thereof, a viscosity agent, and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, a composition provided herein comprises aceclidine or a salt thereof, a (nonionic) surfactant, and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5. In some embodiments, a composition provided herein comprises aceclidine or a salt thereof, a (nonionic) surfactant, a viscosity agent, and a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5.

[0173] In certain embodiments, an individual treated according to a method provided herein has moderate presbyopia. In certain embodiments, an individual treated according to a method provided herein has advanced presbyopia.

[0174] In certain embodiments, an individual treated according to a method provided herein has an improvement in near vision (e.g., at 40 cm) of at least 4 lines. In certain embodiments, the individual has an improvement in near vision (e.g., at 40 cm) of at least 5 lines. In certain embodiments, the individual has an improvement in near vision (e.g., at 40 cm) of at least 6 lines.

[0175] In certain embodiments, an individual treated according to a method provided herein has an improvement in distance vision (e.g., at 4 m) of at least 1 line.

[0176] In certain embodiments, an individual treated according to a method provided herein has an improvement in distance vision (e.g., at 4 m) of at least 3 letters.

[0177] In certain embodiments, an individual treated according to a method provided herein is at least 60 years old. In certain embodiments, the individual is 61-65 years old. In certain embodiments, the individual is 66-70 years old.

[0178] In some embodiments, a method provided herein comprises (e.g., topically) administering a first drop of an ophthalmological composition to an eye (e g., an ocular surface thereof) of an individual and subsequently administering a second drop of the ophthalmological composition to the eye (e.g., an ocular surface thereof) of the individual. In some embodiments, a composition provided herein comprises a composition comprising a miotic (e.g., aceclidine or a salt thereof).

[0179] Provided in certain embodiments herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmological composition to an eye ofthe individual. In specific embodiments, the ophthalmological composition comprises a miotic. In still more specific embodiments, the treated eye of the individual previously underwent ocular surgery

[0180] Provided in certain embodiments herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmological composition to an eye of the individual, the ophthalmological composition comprising aceclidine or a salt thereof.

[0181] Provided in certain embodiments herein, is a method for treating presbyopia and improving distance vision in an individual (e.g., in need thereof), the method comprising opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition provided herein (e.g., comprising a miotic, such as aceclidine or a salt thereof) to the first eye of the individual. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0182] In some embodiments, the treated eye of the individual previously underwent ocular surgery. In some embodiments, an individual treated with a method provided herein previously underwent post-refractive surgery. In some embodiments, an individual treated with a method provided herein previously underwent laser assisted in situ keratomileusis (LASIK) surgery. In some embodiments, an individual treated with a method provided herein previously underwent photorefractive keratectomy (PRK) surgery. In some embodiments, the individual is a pseudophakia individual.

[0183] In certain embodiments, the ophthalmological composition is a pupil selective miotic composition. In some embodiments, the miotic is a pupil selective miotic. In certain embodiments, the pupil selective miotic is aceclidine or an ophthalmically acceptable salt thereof.

[0184] In some embodiments, prior to surgery the individual wore a corrective contact lens or corrective glasses (e.g., bifocals), and after surgery and administration of the ophthalmological composition, the individual does not need to (or does not) wear the corrective contact lens or corrective glasses (e.g., bifocals).

[0185] In some embodiments, following administration of the ophthalmological composition to the eye, the individual has 3-lines or more improvement in the eye (e.g., at 40 cm, by BCDVA). In certain embodiments, following administration of the ophthalmological composition to the eye, the individual has 3-lines or more improvement in the eye within 0.5 hours of administering the second drop to the eye (e.g., at 40 cm, by BCDVA). In some embodiments, following administration of the ophthalmological composition to the eye, the individual has 3-lines or moreimprovement for at least 8 hours in the eye (e.g., at 40 cm, by BCDVA). In specific embodiments, following administration of the ophthalmological composition to the eye, the individual has 3- lines or more improvement for at least 10 hours in the eye (e.g., at 40 cm, by BCDVA).

[0186] In some embodiments, following administration of the ophthalmological composition to the eye, the pupil size of the eye is about 1.5mm to about 2 mm. In specific embodiments, following administration of the ophthalmological composition to the eye, the pupil size of the eye is about 1.5mm to about 2 mm within 0.5 hours of administering the second drop to the eye. In certain embodiments, following administration of the ophthalmological composition to the eye, the pupil size of the eye is about 1.5mm to about 2 mm for at least 8 hours. In specific embodiments, following administration of the ophthalmological composition to the eye, the pupil size of the eye is about 1.5mm to about 2 mm for about 10 hours or more. In some embodiments, following administration of the ophthalmological composition to the eye, the pupil size of the eye is about 1.5mm to about 2 mm for at least 8 hours and no more than about 2.1 mm for at least 10 hours.

[0187] In certain embodiments, the reduction in pupil size is maintained in low light conditions.

[0188] In some embodiments, the reduction in pupil size is maintained for an entire workday (e g., over a period of about 8 hours).

[0189] In certain embodiments, a miotic provided herein is aceclidine or a salt thereof. In some embodiments, an ophthalmological composition provided herein comprises a miotic, such as aceclidine or a salt thereof, at a concentration of about 1.2 wt. % to about 1.8 wt. %. In specific embodiments, the ophthalmological composition comprises aceclidine in a free base concentration of about 1.4 wt. % to about 1.5 wt. %. In some embodiments, the ophthalmological composition is formulated with about 1.7 wt. % to about 1.8 wt. % aceclidine hydrochloride.

[0190] In some embodiments, an ophthalmological composition provided herein does not comprise a cycloplegic. In some embodiments, an ophthalmological composition provided herein does not comprise tropicamide.

[0191] In certain embodiments, an ophthalmological composition is (substantially) preservative- free (e.g., less than 0.2 wt. %, less than 0.1 wt. %, or 0 wt. %). In specific embodiments, the composition does not comprise a preservative.

[0192] In some embodiments, an ophthalmological composition provided herein is clear and colorless.

[0193] In some embodiments, an ophthalmological composition provided herein ophthalmological composition is sterile.

[0194] In some embodiments, an ophthalmological composition provided herein is aqueous.

[0195] In some embodiments, an ophthalmological composition provided herein comprises a thickening agent. In specific embodiments, the thickening agent is hydroxypropyl methylcellulose. In some embodiments, the thickening agent is present in the ophthalmological composition in a concentration of about 1 wt. % to about 1.5 wt. % (e.g., about 1.25 wt. %).

[0196] In some embodiments, an ophthalmological composition provided herein comprises a tonicity agent. In specific embodiments, the tonicity agent is mannitol. In some embodiments, a tonicity agent is present in the ophthalmological composition in a concentration of about 2 wt. % to about 3 wt. % (e.g., about 2.5 wt. %).

[0197] In some embodiments, an ophthalmological composition provided herein comprises a lubricant. In specific embodiments, the lubricant is polysorbate 80. In some embodiments, the lubricant is present in the ophthalmological composition in a concentration of about 3 wt. % to about 5 wt. % (e.g., about 4 wt. %).

[0198] In some embodiments, an ophthalmological composition provided herein comprises a chelating agent. In specific embodiments, the chelating agent is edetate (e.g., formulated with edetate disodium dihydrate). In some embodiments, the chelating agent is present or formulated into the ophthalmological composition in a concentration of about 0.05 wt. % to about 0.2 wt. % (e.g., formulated with edetate disodium dihydrate at a concentration of about 0.1 wt. %).

[0199] In some embodiments, an ophthalmological composition provided herein comprises a buffering agent. In specific embodiments, the buffering agent is citrate (e.g., formulated with sodium citrate). In some embodiments, the buffering agent is present or formulated into the ophthalmological composition in a concentration of about 0.05 wt. % to about 0.15 wt. % (e g., formulated with sodium citrate at a concentration of about 0.08 wt. %).

[0200] In some embodiments, an ophthalmological composition provided herein has a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5.

[0201] In some embodiments, an ophthalmological composition provided herein is stored at room temperature (e g., up to 25 °C) (e.g., up to 3 months or up to 6 months) prior to administration. In some embodiments, an ophthalmological composition provided herein is stored at 2 °C to 8 °C (e.g., up to 12 months or up to 18 months) prior to administration. In certain embodiments, an ophthalmological composition provided herein is stored at 2 °C to 8 °C up to 12 months prior to administration. In certain embodiments, an ophthalmological composition provided herein is stored at 2 °C to 8 °C up to 18 months prior to administration. In certain embodiments, an ophthalmological composition provided herein is stored at 2 °C to 8 °C up to 24 months prior to administration.

[0202] In certain embodiments, an ophthalmological composition provided herein comprises at least 95 wt. % of the aceclidine present in the composition prior to storage after the compositionis stored at 2 °C to 8 °C up to 12 months. In certain embodiments, an ophthalmological composition provided herein comprises at least 95 wt. % of the aceclidine present in the composition prior to storage after the composition is stored at 2 °C to 8 °C up to 18 months. In certain embodiments, an ophthalmological composition provided herein comprises at least 95 wt. % of the aceclidine present in the composition prior to storage after the composition is stored at 2 °C to 8 °C up to 24 months.

[0203] In some embodiments, an ophthalmological composition provided herein is stored at a temperature up to 40 °C (e.g., up to 8 days) prior to administration.

[0204] In some embodiments, an ophthalmological composition provided herein is an eye drop (e.g., slightly viscous ophthalmic solution).

[0205] In certain embodiments, two drops of the ophthalmological composition are administered to the eye (e.g., wherein the two drops are administered about two minutes apart).

[0206] In certain embodiments, an individual treated according to a method provided herein is, following treatment, less dependent on or no longer requires wearing a bifocal glasses, progressive glasses, reading glasses, or a contact lens to correct near vision. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +0.75. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +1.5. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +2.0. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +2.5. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +3.0. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +3.5.

[0207] In certain embodiments, an individual treated according to a method provided herein has moderate presbyopia. In certain embodiments, an individual treated according to a method provided herein has advanced presbyopia.

[0208] In certain embodiments, an individual treated according to a method provided herein has an improvement in near vision (e.g., at 40 cm) of at least 4 lines. In certain embodiments, the individual has an improvement in near vision (e.g., at 40 cm) of at least 5 lines. In certain embodiments, the individual has an improvement in near vision (e.g., at 40 cm) of at least 6 lines.

[0209] In certain embodiments, an individual treated according to a method provided herein has an improvement in distance vision (e.g., at 4 m) of at least 1 line.

[0210] In certain embodiments, an individual treated according to a method provided herein has an improvement in distance vision (e.g., at 4 m) of at least 3 letters.[2H] In certain embodiments, an individual treated according to a method provided herein is at least 60 years old. In certain embodiments, the individual is 61-65 years old. In certain embodiments, the individual is 66-70 years old.

[0212] Provided in certain embodiments herein are systems, compositions, and methods, such as for improving ocular function, such as reducing contact lens wear time and / or treating presbyopia. In specific embodiments, provided herein are systems, compositions, and methods for reducing contact lens wear time.

[0213] In some embodiments, provided herein is a method of treating presbyopia in an individual wearing a contact lens. In certain embodiments, the method comprises administering (e g., topically) one or more drop of an ophthalmological composition comprising aceclidine to an eye of the individual described herein. In specific embodiments, the method comprises removing the contact lens from the eye before administering the ophthalmological composition to the eye. In more specific embodiments, the method comprises reinserting the contact lens onto the eye (e g., about 10 minutes or more) after administering (e.g., dosing) the ophthalmological composition.

[0214] Provided in certain embodiments herein are systems, compositions, and methods, such as for treating ocular disorders, such as presbyopia. In some embodiments, provided herein is a method of improving vision. In some embodiments, provided herein is a method of improving near vision. In some embodiments, provided herein is a method of improving distance vision. In some embodiments, provided herein is a method of treating presbyopia. In some embodiments, a composition provided herein comprises a composition comprising a miotic (e.g., aceclidine or a salt thereof). In some embodiments, a system provided herein comprises such a composition (e g., and a vessel containing the composition). In some embodiments, a method provided herein comprises administering such a composition, such as to an eye (e.g., surface) of an individual.

[0215] Provided in some embodiments herein is a method of treating presbyopia in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0216] Provided in some embodiments herein is a method of treating presbyopia with a concomitant improvement in myopia in an individual (e g., in need thereof). In certain embodiments provided herein is a method of treating presbyopia with a concomitant improvement in myopia in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0217] Provided in some embodiments herein is a method of treating presbyopia with a concomitant improvement in distance vision in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of treating presbyopia with a concomitant improvementin distance vision in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0218] In some embodiments, a method provided herein comprises (e.g., topically) administering a first drop of an ophthalmological composition to an eye (e.g., an ocular surface thereof) of an individual and subsequently administering a second drop of the ophthalmological composition to the eye (e g., an ocular surface thereof) of the individual. In some embodiments, a composition provided herein comprises a composition comprising a miotic (e.g., aceclidine or a salt thereof).

[0219] Provided in certain embodiments herein, is a method of treating presbyopia in an individual wearing a contact lens, the method comprising: administering (e.g., topically) one or more drop of an ophthalmological composition comprising aceclidine to an eye of the individual; removing the contact lens from the eye before administering the ophthalmological composition to the eye; and reinserting the contact lens onto the eye about 10 minutes or more after administering (e.g., dosing) the ophthalmological composition.

[0220] Provided in certain embodiments herein, is a method for treating presbyopia in an individual (e.g., in need thereof), the method comprising opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition provided herein (e.g., comprising a miotic, such as aceclidine or a salt thereof) to the first eye of the individual. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0221] Provided in certain embodiments herein, is a method for treating presbyopia and improving distance vision in an individual (e.g., in need thereof), the method comprising opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition provided herein (e.g., comprising a miotic, such as aceclidine or a salt thereof) to the first eye of the individual. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0222] In some embodiments, provided herein is a method of reducing a corrective lens (e.g., bifocal or contact lens) wear time in an individual, the method comprising administering a composition comprising a miotic to an eye of the individual. In some embodiments, provided herein is a method of reducing a corrective lens (e.g., corrective glasses, such as bifocal, or contactlens) wear time in an individual, the method comprising administering a composition comprising a miotic to an eye of the individual.

[0223] In some embodiments, a lens is a bifocal glasses, progressive glasses, or a contact lens

[0224] In certain embodiments, an individual treated according to a method provided herein is less dependent on wearing bifocals (e.g., glasses, progressive glasses, reading glasses), and / or contacts, such as during the workday. In certain embodiments, an individual treated according to a method provided herein is less dependent on wearing correctives glasses (e.g., bifocal glasses, progressive glasses, reading glasses), and / or contacts, such as during the workday.

[0225] In some embodiments, an individual treated according to a method provided herein no longer requires wearing a bifocal glasses, progressive glasses, reading glasses, or a contact lens (e.g., to correct vision). In certain embodiments, an individual treated according to a method provided herein prior to administration regularly wears corrective lens or at least half a day (e g., during a workday, such as an 8 hour workday) and after administration no longer wears (or needs to wear) a corrective lens during the day. In some embodiments, the individual prior to administration regularly wears a multi-powered corrective lens or at least half a day (e.g., during a workday, such as an 8 hour workday) and after administration no longer wears (or needs to wear) a multi-powered corrective lens during the day (e.g., prior to administration wears a bifocal or progressive glasses and after administration wears a single powered lens such as for treating myopia or astigmatism).

[0226] In some embodiments, an individual treated according to a method provided herein is a post-refractive surgery individual and wherein prior to administration and refractive surgery regularly wore a multi -powered corrective lens or at least half a day (e.g., during a workday, such as an 8 hour workday) and after administration and refractive surgery no longer wears (or needs to wear) a corrective lens during the day (e.g., prior to administration wears a bifocal or progressive glasses and after administration no longer wears any corrective lens).

[0227] In some embodiments, an ophthalmological composition provided herein and / or administered according to a method provided herein is a pupil selective miotic composition. In some embodiments, the miotic is a pupil selective miotic. In specific embodiments, the pupil selective miotic is aceclidine or an ophthalmically acceptable salt thereof.

[0228] In certain embodiments, following administration of the ophthalmological composition according to a method provided herein to the eye, the individual has 3 -lines or more improvement in the eye (e.g., at 40 cm, by BCDVA). In some embodiments, following administration of the ophthalmological composition to the eye, the individual has 3 -lines or more improvement in the eye within 0.5 hours of administering the second drop to the eye. In certain embodiments, following administration of the ophthalmological composition to the eye, the individual has 3-lines or more improvement for at least 8 hours in the eye. In specific embodiments, following administration of the ophthalmological composition to the eye, the individual has 3 -lines or more improvement for at least 10 hours in the eye.

[0229] In some embodiments, following administration of the ophthalmological composition to the eye according to a method provided herein, the pupil size of the eye is about 1.5mm to about 2 mm. In specific embodiments, following administration of the ophthalmological composition to the eye, the pupil size of the eye is about 1.5mm to about 2 mm within 0.5 hours of administering the second drop to the eye. In some embodiments, following administration of the ophthalmological composition to the eye, the pupil size of the eye is about 1.5mm to about 2 mm for at least 8 hours. In specific embodiments, following administration of the ophthalmological composition to the eye, the pupil size of the eye is about 1.5mm to about 2 mm for at least 10 hours. In some embodiments, following administration of the ophthalmological composition to the eye, the pupil size of the eye is about 1.5mm to about 2 mm for at least 8 hours and no more than about 2.1 mm for at least 10 hours. In some embodiments, the reduction in pupil size is maintained in low light conditions.

[0230] In certain embodiments, a composition provided herein comprises a miotic. In specific embodiments, the miotic is aceclidine or a salt thereof. In still more specific embodiments, aceclidine or salt thereof is present in the ophthalmological composition at a concentration of about 1.2 wt. % to about 1.8 wt. %. In some embodiments, the aceclidine is present in the ophthalmological composition at a free base concentration of about 1.4 wt. % to about 1.5 wt. %. In some embodiments, the ophthalmological composition is formulated with about 1.7 wt. % to about 1.8 wt. % aceclidine hydrochloride.

[0231] In certain embodiments, an ophthalmological composition provided herein does not comprise a cycloplegic. In certain embodiments, an ophthalmological composition provided herein does not comprise tropicamide.

[0232] In certain embodiments, an ophthalmological composition provided herein is preservative- free.

[0233] In certain embodiments, an ophthalmological composition provided herein is clear and colorless.

[0234] In certain embodiments, an ophthalmological composition provided herein is sterile.

[0235] In certain embodiments, an ophthalmological composition provided herein is aqueous.

[0236] In certain embodiments, an ophthalmological composition provided herein comprises a thickening agent. In specific embodiments, the thickening agent is hydroxypropyl methylcellulose. In some embodiments, the thickening agent is present in the ophthalmological composition in a concentration of about 1 wt. % to about 1.5 wt. % (e.g., about 1.25 wt. %).

[0237] In certain embodiments, an ophthalmological composition provided herein comprises a tonicity agent. In specific embodiments, the tonicity agent is mannitol. In more specific embodiments, the tonicity agent is present in the ophthalmological composition in a concentration of about 2 wt. % to about 3 wt. % (e.g., about 2.5 wt. %).

[0238] In certain embodiments, an ophthalmological composition provided herein comprises a lubricant. In specific embodiments, the lubricant is polysorbate 80. In still more specific embodiments, the lubricant is present in the ophthalmological composition in a concentration of about 3 wt. % to about 5 wt. % (e.g., about 4 wt. %).

[0239] In certain embodiments, an ophthalmological composition provided herein comprises a chelating agent. In specific embodiments, the chelating agent is edetate (e.g., formulated with edetate disodium dihydrate). In more specific embodiments, the chelating agent is present or formulated into the ophthalmological composition in a concentration of about 0.05 wt. % to about 0.2 wt. % (e.g., formulated with edetate disodium dihydrate at a concentration of about 0.1 wt. %).

[0240] In certain embodiments, an ophthalmological composition provided herein comprises a buffering agent. In specific embodiments, the buffering agent is citrate (e.g., formulated with sodium citrate). In more specific embodiments, the buffering agent is present or formulated into the ophthalmological composition in a concentration of about 0.05 wt. % to about 0.15 wt. % (e.g., formulated with sodium citrate at a concentration of about 0.08 wt. %).

[0241] In certain embodiments, an ophthalmological composition provided herein has a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5.

[0242] In certain embodiments, an ophthalmological composition provided herein is stored at room temperature (e.g., up to 25 °C) (e.g., up to 3 months, such as for 2-3 months, or up to 6 months) prior to administration.

[0243] In certain embodiments, an ophthalmological composition provided herein is stored at 2 °C to 8 °C (e.g., up to 12 months or up to 18 months, such as for 12-18 months) prior to administration. In certain embodiments, an ophthalmological composition provided herein is stored at 2 °C to 8 °C up to 12 months prior to administration. In certain embodiments, an ophthalmological composition provided herein is stored at 2 °C to 8 °C up to 18 months prior to administration. In certain embodiments, an ophthalmological composition provided herein is stored at 2 °C to 8 °C up to 24 months prior to administration.

[0244] In certain embodiments, an ophthalmological composition provided herein comprises at least 95 wt. % of the aceclidine present in the composition prior to storage after the composition is stored at 2 °C to 8 °C up to 12 months. In certain embodiments, an ophthalmological composition provided herein comprises at least 95 wt. % of the aceclidine present in thecomposition prior to storage after the composition is stored at 2 °C to 8 °C up to 18 months. In certain embodiments, an ophthalmological composition provided herein comprises at least 95 wt. % of the aceclidine present in the composition prior to storage after the composition is stored at 2 °C to 8 °C up to 24 months.

[0245] In certain embodiments, an ophthalmological composition provided herein is stored at a temperature up to 40 °C (e.g., up to 8 days) prior to administration.

[0246] In certain embodiments, an ophthalmological composition provided herein is an eye drop (e.g., slightly viscous ophthalmic solution). In certain embodiments, an ophthalmological composition provided herein, wherein two drops of the ophthalmological composition are administered to the eye (e.g., wherein the two drops are administered about two minutes apart).

[0247] In certain embodiments, an individual treated according to a method provided herein is, following treatment, less dependent on or no longer requires wearing a bifocal glasses, progressive glasses, reading glasses, or a contact lens to correct near vision. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +0.75. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +1.5. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +2.0. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +2.5. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +3.0. In certain embodiments, the bifocal glasses, progressive glasses, reading glasses, or contact lens corrects near vision by at least +3.5.

[0248] In certain embodiments, an individual treated according to a method provided herein has moderate presbyopia. In certain embodiments, an individual treated according to a method provided herein has advanced presbyopia.

[0249] In certain embodiments, an individual treated according to a method provided herein has an improvement in near vision (e.g., at 40 cm) of at least 4 lines. In certain embodiments, the individual has an improvement in near vision (e.g., at 40 cm) of at least 5 lines. In certain embodiments, the individual has an improvement in near vision (e.g., at 40 cm) of at least 6 lines.

[0250] In certain embodiments, an individual treated according to a method provided herein has an improvement in distance vision (e.g., at 4 m) of at least 1 line.

[0251] In certain embodiments, an individual treated according to a method provided herein has an improvement in distance vision (e.g., at 4 m) of at least 3 letters.

[0252] In certain embodiments, an individual treated according to a method provided herein is at least 60 years old. In certain embodiments, the individual is 61-65 years old. In certain embodiments, the individual is 66-70 years old.

[0253] Provided in certain embodiments herein are systems, compositions, and methods, such as for improving ocular function, such as increasing comfortable screen time and / or treating presbyopia. In specific embodiments, provided herein are systems, compositions, and methods for increasing comfortable screen time.

[0254] Provided in certain embodiments herein are systems, compositions, and methods, such as for treating ocular disorders, such as presbyopia. In some embodiments, provided herein is a method of improving vision. In some embodiments, provided herein is a method of improving near vision. In some embodiments, provided herein is a method of improving distance vision. In some embodiments, provided herein is a method of treating presbyopia. In some embodiments, a composition provided herein comprises a composition comprising a miotic (e.g., aceclidine or a salt thereof). In some embodiments, a system provided herein comprises such a composition (e g., and a vessel containing the composition). In some embodiments, a method provided herein comprises administering such a composition, such as to an eye (e.g., surface) of an individual.

[0255] Provided in some embodiments herein is a method of treating presbyopia in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0256] In some embodiments, a method provided herein comprises (e.g., topically) administering a first drop of an ophthalmological composition to an eye (e g., an ocular surface thereof) of an individual and subsequently administering a second drop of the ophthalmological composition to the eye (e.g., an ocular surface thereof) of the individual. In some embodiments, a composition provided herein comprises a composition comprising a miotic (e.g., aceclidine or a salt thereof).

[0257] In some embodiments, provided herein is a method of increasing comfortable screen time in an individual, the method comprising administering an ophthalmological composition comprising a miotic to an eye of the individual. In some embodiments, increasing comfortable screen time comprises increasing the duration of usage of the computer screen before onset of discomfort (e g., discomfort associated with the eye, such as eye blurriness, eye strain, eye pain, eye irritation, eye associate migraine or headache, computer vision syndrome, or a combination thereof). In certain embodiments, comfortable screen time in the individual lasts for a duration of at least 4 (e.g., consecutive) hours (e.g., 5 hours or more, 6 hours or more, 7 hours or more, or 8 hours or more). In some embodiments, comfortable screen time in the individual lasts for a duration of at least 1 hours (e.g., 2 hours or more, 3 hours or more, 4 hours or more, or 6 hours ormore) more than otherwise would have been achieved in the absence of administration of the ophthalmological composition to the eye. In some embodiments, comfortable screen time in the individual is improved for an entire workday (e g., over a period of about 8 hours) or longer, such as compared to a period of time before administering the ophthalmological composition to the eye. In certain embodiments, increasing comfortable screen time comprises increasing comfortable use of time in a smart phone, computer, electronic tablet, or a combination thereof. In some embodiments, increasing comfortable screen time comprises increasing the duration of usage of the computer screen before onset of eye blurriness, eye strain, eye pain, eye irritation, eye associate migraine or headache, computer vision syndrome, or a combination thereof.

[0258] In some embodiments, an ophthalmic composition provided herein is a pupil selective miotic composition.

[0259] In some embodiments, an ophthalmic composition provided herein comprises a miotic. In specific embodiments, the miotic is a pupil selective miotic. In some embodiments, the pupil selective miotic is aceclidine or an ophthalmically acceptable salt thereof.

[0260] In certain embodiments, following administration of an ophthalmological composition provided herein to an eye, the individual has 3 -lines or more improvement in the eye (e.g., at 40 cm, by BCDVA). In some embodiments, following administration of an ophthalmological composition to the eye, the individual has 3 -lines or more improvement in the eye within 0.5 hours of administering the second drop to the eye. In certain embodiments, following administration of the ophthalmological composition to the eye, the individual has 3-lines or more improvement for at least 8 hours in the eye. In some embodiments, following administration of an ophthalmological composition to the eye, the individual has 3-lines or more improvement for at least 10 hours in the eye.

[0261] In some embodiments, following administration of an ophthalmological composition provided herein to an eye, the pupil size of the eye is about 1.5mm to about 2 mm. In some embodiments, following administration of an ophthalmological composition to an eye, the pupil size of the eye is about 1.5mm to about 2 mm within 0.5 hours of administering the second drop to an eye. In certain embodiments, following administration of an ophthalmological composition to an eye, the pupil size of the eye is about 1.5mm to about 2 mm for at least 8 hours. In some embodiments, an ophthalmological composition to the eye, the pupil size of the eye is about 1.5mm to about 2 mm for at least 10 hours. In certain embodiments, following administration of an ophthalmological composition to an eye, the pupil size of the eye is about 1.5mm to about 2 mm for at least 8 hours and no more than about 2.1 mm for at least 10 hours. In some embodiments, reduction in pupil size is maintained in low light conditions.

[0262] In certain embodiments, a composition provided herein comprises a miotic. In specific embodiments, the miotic is aceclidine or a salt thereof and is present in the ophthalmological composition at a concentration of about 1.2 wt. % to about 1.8 wt. %. In some embodiments, aceclidine is present in the ophthalmological composition at a free base concentration of about 1.4 wt. % to about 1.5 wt. %. In some embodiments, an ophthalmological composition provided herein is formulated with about 1.7 wt % to about 1 8 wt. % aceclidine hydrochloride

[0263] In some embodiments, an ophthalmic composition provided herein does not comprise cycloplegic. In some embodiments, an ophthalmic composition provided herein does not comprise tropicamide.

[0264] In some embodiments, an ophthalmic composition provided herein is preservative-free (free of an antimicrobial preservative).

[0265] In some embodiments, an ophthalmic composition provided herein is clear and colorless.

[0266] In some embodiments, an ophthalmic composition provided herein is sterile.

[0267] In some embodiments, an ophthalmic composition provided herein is aqueous.

[0268] In some embodiments, an ophthalmic composition provided herein comprises a thickening agent. In specific embodiments, the thickening agent is hydroxypropyl methylcellulose. In some embodiments, the thickening agent is present in the ophthalmological composition in a concentration of about 1 wt. % to about 1.5 wt. % (e.g., about 1.25 wt. %).

[0269] In some embodiments, an ophthalmic composition provided herein comprises a tonicity agent. In specific embodiments, the tonicity agent is mannitol. In some embodiments, the tonicity agent is present in the ophthalmological composition in a concentration of about 2 wt. % to about 3 wt. % (e.g., about 2.5 wt. %).

[0270] In some embodiments, an ophthalmic composition provided herein comprises a lubricant. In specific embodiments, the lubricant is polysorbate 80. In some embodiments, the lubricant is present in the ophthalmological composition in a concentration of about 3 wt. % to about 5 wt. % (e.g., about 4 wt. %).

[0271] In some embodiments, an ophthalmic composition provided herein comprises a chelating agent. In specific embodiments, the chelating agent is edetate (e g., formulated with edetate disodium dihydrate). In some embodiments, the chelating agent is present or formulated into the ophthalmological composition in a concentration of about 0.05 wt. % to about 0.2 wt. % (e.g., formulated with edetate disodium dihydrate at a concentration of about 0.1 wt. %).

[0272] In some embodiments, an ophthalmic composition provided herein comprises a buffering agent. In specific embodiments, the buffering agent is citrate (e.g., formulated with sodium citrate). In some embodiments, the buffering agent is present or formulated into theophthalmological composition in a concentration of about 0.05 wt. % to about 0.15 wt. % (e.g., formulated with sodium citrate at a concentration of about 0.08 wt. %).

[0273] In some embodiments, an ophthalmic composition provided herein has a pH of about 4.5 to about 5.5, optionally of about 5.0 to about 5.5.

[0274] In some embodiments, an ophthalmic composition provided herein is stored at room temperature (e g., up to 25 °C) (e.g., up to 3 months, such as 2-3 months, or up to 6 months) prior to administration.

[0275] In some embodiments, an ophthalmic composition provided herein is stored at 2 °C to 8 °C (e.g., up to 12 months or up to 18 months, such as 12-18 months) prior to administration. In certain embodiments, an ophthalmological composition provided herein is stored at 2 °C to 8 °C up to 12 months prior to administration. In certain embodiments, an ophthalmological composition provided herein is stored at 2 °C to 8 °C up to 18 months prior to administration. In certain embodiments, an ophthalmological composition provided herein is stored at 2 °C to 8 °C up to 24 months prior to administration.

[0276] In certain embodiments, an ophthalmological composition provided herein comprises at least 95 wt. % of the aceclidine present in the composition prior to storage after the composition is stored at 2 °C to 8 °C up to 12 months. In certain embodiments, an ophthalmological composition provided herein comprises at least 95 wt. % of the aceclidine present in the composition prior to storage after the composition is stored at 2 °C to 8 °C up to 18 months. In certain embodiments, an ophthalmological composition provided herein comprises at least 95 wt. % of the aceclidine present in the composition prior to storage after the composition is stored at 2 °C to 8 °C up to 24 months.

[0277] In some embodiments, an ophthalmic composition provided herein is stored at a temperature up to 40 °C (e.g., up to 8 days, such as 1-8 days) prior to administration.

[0278] In some embodiments, an ophthalmic composition provided herein is an eye drop (e.g., slightly viscous ophthalmic solution).

[0279] In some embodiments, two drops of the ophthalmological composition are administered to the eye (e.g., wherein the two drops are administered about two minutes apart), such as in any method provided herein.

[0280] Provided in certain embodiments herein, is a method for treating presbyopia in an individual (e.g., in need thereof), the method comprising opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition provided herein (e.g., comprising a miotic, such as aceclidine or a salt thereof) to the first eye of the individual. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first dropis administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0281] Provided in certain embodiments herein, is a method for treating presbyopia and improving distance vision in an individual (e.g., in need thereof), the method comprising opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition provided herein (e.g., comprising a miotic, such as aceclidine or a salt thereof) to the first eye of the individual. In some embodiments, the second drop is administered to the eye of the individual about 5 minutes or less after the first drop is administered to the eye. In some embodiments, the second drop is administered to the eye of the individual about 2 minutes after the first drop is administered to the eye.

[0282] In certain embodiments, an individual treated according to a method provided herein has moderate presbyopia. In certain embodiments, an individual treated according to a method provided herein has advanced presbyopia.

[0283] In certain embodiments, an individual treated according to a method provided herein has an improvement in near vision (e.g., at 40 cm) of at least 4 lines. In certain embodiments, the individual has an improvement in near vision (e.g., at 40 cm) of at least 5 lines. In certain embodiments, the individual has an improvement in near vision (e.g., at 40 cm) of at least 6 lines.

[0284] In certain embodiments, an individual treated according to a method provided herein has an improvement in distance vision (e.g., at 4 m) of at least 1 line.

[0285] In certain embodiments, an individual treated according to a method provided herein has an improvement in distance vision (e.g., at 4 m) of at least 3 letters.

[0286] In certain embodiments, an individual treated according to a method provided herein is at least 60 years old. In certain embodiments, the individual is 61-65 years old. In certain embodiments, the individual is 66-70 years old.

[0287] Also provided herein is the use of a buffer to maintain stability of aceclidine in an ophthalmological composition. The ophthalmological composition may comprise any component as described herein. In some embodiments, the ophthalmological composition is preservative-free.

[0288] In some embodiments the buffer is citrate. In some embodiments, the buffer is present in the ophthalmological composition in a concentration of about 0.05 wt. % to about 0.15 wt.%, optionally about 0.06 wt. % to about 0.1 wt. %. In some embodiments, the buffer is sodium citrate. In some embodiments, the sodium citrate buffer is present in the ophthalmological composition in a concentration of about 0.08 wt. %.

[0289] In some embodiments the buffer provides a pH in the ophthalmological composition of about 4.5 to about 5.5, optionally about 5.0 to 5.5.

[0290] In some embodiments, stability of aceclidine is maintained at a temperature of about 2 °C to about 8 °C. In some embodiments, the ophthalmological composition has at least 90%, or at least 95% of the initial amount of aceclidine after storage for 24 months at a temperature of about 2 °C to about 8 °C. In some embodiments, stability of aceclidine is maintained at room temperature. In some embodiments, the ophthalmological composition has at least 90%, at least 95%, or at least 98% initial aceclidine at any point after up to 3 months, or up to 6 months of storage at room temperature. In some embodiments, stability of aceclidine is maintained at 40 °C. In some embodiments, the ophthalmological composition has at least 90%, at least 95%, or at least 98% initial aceclidine at any point after up to 2 months of storage at 40 °C.

[0291] Additional aspects and advantages of the present disclosure will become readily apparent to those skilled in this art from the following detailed description, wherein only illustrative embodiments of the present disclosure are shown and described. As will be realized, the present disclosure is capable of other and different embodiments, and its several details are capable of modifications in various obvious respects, all without departing from the disclosure. Accordingly, the drawings and description are to be regarded as illustrative in nature, and not as restrictive.BRIEF DESCRIPTION OF THE DRAWINGS

[0292] The novel features of the disclosure are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present disclosure will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the disclosure are utilized, and the accompanying drawings (also “Figure” and “FIG.” herein) of which:

[0293] FIG. 1A illustrates the extended stability at temperatures up to 40 °C for an exemplary aceclidine composition described herein comprising a pH of 5.0.

[0294] FIG. IB illustrates the good stability at 0 degrees Celsius (°C) to 40 °C for an exemplary aceclidine composition described herein comprising a pH of 5.5.

[0295] FIG. 1C illustrates the good stability at temperatures up to 40 °C for an exemplary aceclidine composition described herein comprising a pH of 6.0.

[0296] FIG. ID illustrates the stability at temperatures up to 40 °C for an exemplary aceclidine composition described herein comprising a pH of 6.5.

[0297] FIG. 2A illustrates the extended stability at temperatures up to 40 °C for an exemplary aceclidine composition described herein comprising a pH of 5.0.

[0298] FIG. 2B illustrates the extended stability at temperatures up to 40 °C for an exemplary aceclidine composition described herein comprising a pH of 5.5.

[0299] FIG. 2C illustrates the good stability at temperatures up to 40 °C for an exemplary aceclidine composition described herein comprising a pH of 6.0.

[0300] FIG. 2D illustrates the stability at temperatures up to 40 °C for an exemplary aceclidine composition described herein comprising a pH of 6.5.

[0301] FIG. 3A illustrates the extended stability at temperatures up to 40 °C for an exemplary aceclidine composition described herein comprising a pH of 5.0.

[0302] FIG. 3B illustrates the extended stability at temperatures up to 40 °C for an exemplary aceclidine composition described herein comprising a pH of 5.5.

[0303] FIG. 3C illustrates the good stability at temperatures up to 40 °C for an exemplary aceclidine composition described herein comprising a pH of 6.0.

[0304] FIG. 3D illustrates the stability at temperatures up to 40 °C for an exemplary aceclidine composition described herein comprising a pH of 6.5.

[0305] FIG. 4A illustrates the extended stability at temperatures up to 40 °C for an exemplary aceclidine composition described herein comprising a preservative.

[0306] FIG. 4B illustrates the extended stability at temperatures up to 40 °C for an exemplary preservative-free aceclidine composition described herein.

[0307] FIG. 4C, FIG. 4D, and FIG. 4E illustrate excellent stability in such aqueous aceclidine compositions (solutions) in a substantially viscosity agent (HPMC) independent manner.

[0308] FIG. 5 illustrates rapid onset and extended duration of near vision improvement in individuals being treated with exemplary systems and methods described herein.

[0309] FIG. 6 illustrates near universal response of near vision improvement in individuals being treated with exemplary systems and methods described herein.

[0310] FIG. 7 illustrates distance vision improvement in individuals being treated with exemplary systems and methods described herein.

[0311] FIG.8 illustrates consistent near vision improvement over 4 weeks in individuals being treated with exemplary systems and methods described herein.

[0312] FIG. 9 illustrates near vision improvement in individuals with moderate and advanced presbyopia.

[0313] FIG. 10 illustrates distant vision improvement of at least 1 line.

[0314] FIG. 11 illustrates near vision improvement of at least 4 lines and up to 8 lines in individuals with presbyopia.

[0315] FIG. 12 illustrates a pair of exemplary single use containers that when taken together with a composition provided herein forms an exemplary system provided herein.DETAILED DESCRIPTION

[0316] Whenever the term “at least,” “greater than,” or “greater than or equal to” precedes the first numerical value in a series of two or more numerical values, the term “at least,” “greater than” or “greater than or equal to” applies to each of the numerical values in that series of numerical values. For example, greater than or equal to 1, 2, or 3 is equivalent to greater than or equal to 1, greater than or equal to 2, or greater than or equal to 3.

[0317] Whenever the term “up to,” “no more than,” “less than,” or “less than or equal to” precedes the first numerical value in a series of two or more numerical values, the term “no more than,” “less than,” or “less than or equal to” applies to each of the numerical values in that series of numerical values. For example, less than or equal to 3, 2, or 1 is equivalent to less than or equal to 3, less than or equal to 2, or less than or equal to 1.

[0318] In some instances, a value is “about” a recited value if the value is within ±10% of the recited value. For example, if it is stated, that “the temperature is about 20 “Celsius” a value may be “about” 20 °C if it is within ±10% of 20 °C, or from 18 °C to 22 °C. In more specific instances, a value is “about” a recited value if the value is within ±5% of the recited value. In some instances, a value is “about” a recited value if the value is within ± one significant figure of the recited value. For example, if it is stated, that a concentration is about 2.0 wt. %, the concentration may be about 2.0 wt. % if it is within ± one significant figure of 2.0 wt. %, or from 1.9 wt. % to about 2.1 wt. %. It is also understood that any disclosure provided herein to “about” a number, also includes disclosure of that number itself (i.e., without “about”).

[0319] The term “comprise” and variations thereof, such as, “comprises” and “comprising” are to be construed in an open, inclusive sense, that is, as “including, but not limited to.” It is also to be understood that disclosure of “comprising” is also intended to include disclosures of “consisting essentially of’ and “consisting of’ the same recited elements. Generally, “consisting essentially of’ is to construed at partially open, being directed to those elements set forth and to those other elements that do not materially affect the basic and novel characteristics of the invention described. Generally, “consisting of’ means that the claim is directed only to the specified elements.

[0320] As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the content clearly dictates otherwise. It should also be noted that the term “or” is generally employed in its sense including “and / or” unless the content clearly dictates otherwise.

[0321] The term “optional” or “optionally” denotes that a subsequently described event or circumstance can but need not occur, and that the description includes instances where the event or circumstance occurs and instances in which it does not.

[0322] The term “amount of’ a component as used herein refers to “volume of’ and / or “mass of’ of the particular component.

[0323] As used herein, the term “weight percent” or “wt. %” refers to weight-weight percent (% w / w) or weight-volume percent (% w / v). In some instances, compositions provided herein comprise or are said to comprise various compounds and salts. In certain instances, compositions provided herein comprise a solvent, such as an aqueous medium. In some instances, compositions provided herein may be described as comprising a salt. In certain instances, the salt of a composition (e.g., aqueous composition) provided herein may be partially or completely dissolved in the composition. However, it is to be understood that irrespective of the form, a composition provided herein that is formulated with a salt comprises that salt, irrespective of whether or not that salt is partially or wholly dissolved in that composition. In some instances, for example, an aqueous composition provided herein that is said to be comprising an aceclidine hydrochloride in an amount of about 1.75 wt. % is understood to comprise aceclidine hydrochloride if (a) the composition was formulated using aceclidine hydrochloride in the appropriate amount (about 1.75 wt. %), or (b) the composition comprises an amount of aceclidine free base (or protonated cation) and a sufficient amount of chloride in the composition that would correspond to aceclidine hydrochloride in an amount of about 1.75 wt. %.

[0324] Provided herein is a composition comprising an ophthalmological drug described herein (e.g., aceclidine) and having a pH of 6 or less, such as a pH of about 4 to about 6. In some embodiments, the composition has a pH of about 4.5 to about 6. In some embodiments, the composition has a pH of about 4.5 to about 5.5. In some embodiments, the composition has a pH of about 5.0 to about 5.5. In some embodiments, the composition has a pH of about 5 to about 6. In some embodiments, the composition has a pH of about 5.0 to about 5.5. In some embodiments, the composition has a pH of about 5.5 to about 6. In some embodiments, the composition has a pH of about 5. In some embodiments, the composition has a pH of about 5.5. In some embodiments, the composition further comprises a buffer (e.g., an acetate buffer or a citrate buffer), such as at a concentration of about 0.01% to about 1% (e.g., about 0.05% to about 0.2%). In some embodiments, the composition is suitable for ophthalmic administration (e.g., ophthalmically acceptable or ophthalmologically acceptable). In some embodiments, an ophthalmological drug described herein is aceclidine or a salt thereof.

[0325] Provided in certain embodiments herein are systems, compositions, and methods, such as for improving ocular function, such as reducing corrective lens wear time and / or treating presbyopia. In specific embodiments, provided herein are systems, compositions, and methods for reducing corrective lens wear time.

[0326] Provided in certain embodiments herein are systems, compositions, and methods, such as for treating ocular disorders, such as presbyopia. In some embodiments, an individual described herein does not have or has not been diagnosed with glaucoma. In some embodiments, an individual described herein has presbyopia and does not have or has not been diagnosed with glaucoma.

[0327] In some embodiments, provided herein is a method of treating short distance vision

[0328] In some aspects, provided herein is a method of treating presbyopia in an individual using any composition of the disclosure. In some embodiments, the method of treating presbyopia comprises administering the composition to the eye of an individual.

[0329] Provided in some embodiments herein is a method of treating presbyopia in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmological composition to an eye(e.g., an ocular surface thereof) of the individual.

[0330] Provided in some embodiments herein is a method of treating presbyopia in an individual (e.g., in need thereof), wherein the individual wears a corrective lens (e g., a contact lens, reading glasses, or progressive glasses). In certain embodiments provided herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual. In some embodiments, the individual wears reading glasses having a power of at least +0.75. In some embodiments, the individual wears reading glasses having a power of at least +1.0. In some embodiments, the individual wears reading glasses having a power of at least +1.5. In some embodiments, the individual wears reading glasses having a power of at least +2.0. In some embodiments, the individual wears reading glasses having a power of at least +2.5. In some embodiments, the individual wears reading glasses having a power of at least +3.0. In some embodiments, the individual wears reading glasses having a power of at least +3.5. In certain embodiments, the wears a progressive lens with a near vision correction of any reading glasses described herein.

[0331] Provided in some embodiments herein is a method of reducing corrective lens usage in an individual (e.g., in need thereof), wherein the individual wears a corrective lens (e.g., a contact lens, reading glasses, or progressive glasses). In certain embodiments provided herein is a method of treating presbyopia and / or reducing corrective lens usage in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual. In some embodiments, the individual wears reading glasses having a power of at least +0.75. In some embodiments, the individual wears reading glasses having a power of at least +1.0. In some embodiments, the individual wears reading glasses having a powerof at least +1.5. In some embodiments, the individual wears reading glasses having a power of at least +2.0. In some embodiments, the individual wears reading glasses having a power of at least +2.5 In some embodiments, the individual wears reading glasses having a power of at least +3.0. In some embodiments, the individual wears reading glasses having a power of at least +3.5. In certain embodiments, the individual wears a progressive lens with a near vision correction of any reading glasses described herein.

[0332] Provided in some embodiments herein is a method of treating presbyopia with a concomitant improvement in myopia in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of treating presbyopia with a concomitant improvement in myopia in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0333] Provided in some embodiments herein is a method of treating presbyopia with a concomitant improvement in distance vision in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of treating presbyopia with a concomitant improvement in distance vision in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0334] In some aspects, provided herein is a method of treating an eye condition in an individual using any composition of the disclosure. In some embodiments, an eye condition comprises presbyopia, myopia, myopic blur, irregular astigmatism, or a refractive error of the eye. In some embodiments, an eye condition does not comprise glaucoma. In some embodiments, provided herein is a method of improving short distance vision. In some embodiments, the method comprises administering the composition to the eye of an individual, where the composition is at a room temperature.

[0335] In some embodiments, increasing comfortable screen time comprises increasing the duration of usage of the computer screen. In specific embodiments, increasing comfortable screen time comprises increasing the duration of usage of the computer screen before onset of discomfort (e.g., discomfort associated with the eye). In more specific embodiments, discomfort associated with the eye is eye blurriness, eye strain, eye pain, eye irritation, eye associate migraine or headache, computer vision syndrome, or a combination thereof.

[0336] In some embodiments, comfortable screen time in the individual lasts for a duration of about 2 (e.g., continuous) hours or more. In some embodiments, comfortable screen time in the individual lasts for a duration of about 3 (e.g., continuous) hours or more. In some embodiments, comfortable screen time in the individual lasts for a duration of about 4 (e g., continuous) hours or more. In some embodiments, comfortable screen time in the individual lasts for a duration of about 5 (e.g., continuous) hours or more. In some embodiments, comfortable screen time in theindividual lasts for a duration of about 6 (e.g., continuous) hours or more. In some embodiments, comfortable screen time in the individual lasts for a duration of about 7 (e g., continuous) hours or more. In some embodiments, comfortable screen time in the individual lasts for a duration of about 8 (e.g., continuous) hours or more.

[0337] In some embodiments, comfortable screen time in the individual lasts for a duration of about 30 (e g., continuous) minutes or more than otherwise would have been achieved in the absence of administration of an ophthalmological composition provided herein to the eye. In some embodiments, comfortable screen time in the individual lasts for a duration of about 1 (e g., continuous) hours or more than otherwise would have been achieved in the absence of administration of an ophthalmological composition provided herein to the eye. In some embodiments, comfortable screen time in the individual lasts for a duration of about 2 (e g., continuous) hours or more than otherwise would have been achieved in the absence of administration of an ophthalmological composition provided herein to the eye. In some embodiments, comfortable screen time in the individual lasts for a duration of about 3 (e.g., continuous) hours or more than otherwise would have been achieved in the absence of administration of an ophthalmological composition provided herein to the eye. In some embodiments, comfortable screen time in the individual lasts for a duration of about 4 (e.g., continuous) hours or more than otherwise would have been achieved in the absence of administration of an ophthalmological composition provided herein to the eye. In some embodiments, comfortable screen time in the individual lasts for a duration of about 5 (e.g., continuous) hours or more than otherwise would have been achieved in the absence of administration of an ophthalmological composition provided herein to the eye. In some embodiments, comfortable screen time in the individual lasts for a duration of about 6 (e.g., continuous) hours or more than otherwise would have been achieved in the absence of administration of an ophthalmological composition provided herein to the eye. In some embodiments, comfortable screen time in the individual lasts for a duration of about 7 (e.g., continuous) hours or more than otherwise would have been achieved in the absence of administration of an ophthalmological composition provided herein to the eye.

[0338] In some embodiments, comfortable screen time in an individual described herein is improved, such as to last for an entire workday (e.g., over a period of about 8 hours) or longer.

[0339] In some embodiments, comfortable screen time in an individual described herein is improved compared to a period of time before administering an ophthalmological composition provided herein to the eye.

[0340] In some embodiments, increasing comfortable screen time comprises increasing comfortable use of time of any suitable electronic device (e.g., with a screen). In specificembodiments, increasing comfortable screen time comprises increasing comfortable use of time of a smart phone, computer, electronic tablet, or a combination thereof.

[0341] In some embodiments, a method provided herein comprises (e g., topically) administering a first drop of an ophthalmological composition to an eye (e.g., an ocular surface thereof) of an individual and subsequently administering a second drop of the ophthalmological composition to the eye (e g., an ocular surface thereof) of the individual.

[0342] In some embodiments, a first drop and a second drop of an ophthalmological composition (e.g., provided herein) is administered to an eye of an individual within less than 5 minutes of each other. In specific embodiments, the first drop and the second drop are administered within 3 minutes of each other. In more specific embodiments, the second drop is administered about 1 to about 3 minutes after the first drop. In still more specific embodiments, the second drop is administered about 2 minutes after the first drop. In yet more specific embodiments, the second drop is administered 2 minutes after the first drop. In some embodiments, a drop is administered in a first eye and a second eye immediately (e.g., within 15 seconds) after the drop is administered to the first eye and about 2 minutes later, another drop is administered in a first eye and a second eye immediately (e.g., within 15 seconds) after the drop is administered to the first eye. In some instances, it is surprising that extended duration of the effect of the method can be achieved by administering 2 drops within 5 minutes of each other, including as little as about 2 minutes of each other. In certain instances, administration of 2 drops within as little as about 2 minutes can improve patient compliance with the administration protocol (e.g., of 2 drops administration) and / or decrease potential for contamination of an open composition between administration of the 2 drops for a longer period.

[0343] Provided in some embodiments herein is a method of treating presbyopia in an individual (e.g., in need thereof). In some embodiments, a method provided herein consists essentially of (e.g., topically) administering (e.g., instilling) a first drop of an ophthalmological composition to an eye (e.g., an ocular surface thereof) of an individual and subsequently administering a second drop of the ophthalmological composition to the eye (e.g., an ocular surface thereof) of the individual, wherein the second drop is administered within about 2 minutes of the first drop. In specific embodiments, the second drop is administered about 2 minutes after the first drop.

[0344] Provided in some embodiments herein is a method of treating presbyopia in an individual (e.g., in need thereof), the method comprising (e.g., topically) administering a dose of an ophthalmological composition to an eye (e.g., an ocular surface thereof) of an individual. In certain embodiments, the dose of the ophthalmological composition comprises a first drop of the ophthalmological composition and subsequently administering a second drop of the ophthalmological composition. In some embodiments, the first drop and the second drop areadministered at any suitable frequency, such as a frequency described herein (e.g., a second drop being administered 2 minutes after the first drop).

[0345] In some embodiments provided herein is a method for treating presbyopia, the method comprising administering a dose once daily. In some embodiments provided herein is a method for treating presbyopia, the method consisting essentially of administering a dose once daily. In some embodiments, the once daily dose is administered 5 days a week (or any suitable number of days a week), such as during the work week.

[0346] In some embodiments provided herein is a method for treating presbyopia, the method comprising administering a dose on a first day and a second day. In some embodiments, the first day dose comprises administering a first drop and a second drop on the first day. In some embodiments, the second day dose comprises administering a third drop and a fourth drop on the second day.

[0347] In some embodiments provided herein is a method for treating presbyopia, the method comprising administering a first dose (e.g., a first drop and a second drop is administered, such as about 2 minutes apart) to a first eye of an individual and a second dose (e g., a third drop and a fourth drop is administered, such as about 2 minutes apart) to a second eye of an individual. In certain embodiments, (i) a first dose is administered to a first eye on a first day, (ii) a second dose is administered to a second eye on the first day, (iii) a third dose is administered to the first eye on a second day, and (iv) a fourth dose is administered to the second eye on the second day.

[0348] In some embodiments provided herein is a system comprising a (e.g., single-use) container and an ophthalmological composition. In certain embodiments, provided herein is a method of using any system or composition provided herein, such as improving vision (e g., improving near vision, or improving distance vision). In certain embodiments, provided herein is a method of using any system or composition provided herein, such as in the treatment of presbyopia and / or reducing corrective lens wear time. In some embodiments, the method comprises administering a composition provided herein. In certain embodiments, provided herein is a method comprising opening a container of a system provided herein and dispensing a composition provided herein.

[0349] Provided in some embodiments herein is a method of improving vision (e.g., improving near vision, or improving distance vision) in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of improving vision (e.g., improving near vision, or improving distance vision) in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0350] Provided in some embodiments herein is a method of improving near vision in an individual (e.g., in need thereof). In certain embodiments provided herein is a method ofimproving near vision in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0351] Provided in some embodiments herein is a method of improving distance vision in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of improving distance vision in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0352] Provided in some embodiments herein is a method of improving near vision and improving distance vision in an individual (e.g., in need thereof). In certain embodiments provided herein is a method of improving near vision and improving distance vision in an individual, the method comprising administering an ophthalmological composition to an eye (e.g., an ocular surface thereof) of the individual.

[0353] In some embodiments, a single-use container (also referred interchangeably herein as a single-patient-use container) comprises an enclosed chamber. In specific embodiments, any ophthalmological composition provided herein is configured within the enclosed chamber (of a single use container described herein).

[0354] In certain embodiments, the single-use container is configured to be opened in any suitable manner. In specific embodiments, the single-use container is configured to be irreversibly opened (e.g., wherein when opened, the chamber of the single-use container is no longer enclosed, instead having an opening having fluid connectivity to the exterior of the chamber). In some embodiments, a single-use container provided herein is configured when open (or after being opened) to dispense (e g., from the chamber of the single-use container to the exterior of the chamber or container) at least one drop of any composition, such as a composition provided herein.

[0355] In some embodiments, a method provided herein comprises opening a single-use container, such as provided herein, administering a first drop and a second drop (e.g., about 2 minutes apart) (e.g., a first dose) of an ophthalmological composition from the single-use container to a first eye of an individual. In specific embodiments, the method comprises administering a third drop and a fourth drop (e.g., about 2 minutes apart) (e.g., a second dose) of the ophthalmological composition from the single-use container to a second eye of an individual. In certain embodiments, such administration can occur on multiple days, a first day and a second day, or other suitable frequency, such as daily or every workday or the like.

[0356] In some embodiments, a container provided or used herein comprises a container body, a container neck, and a container head (e.g., wherein the head and body are connected at the neck). In specific embodiments, the container body and the container head having a larger cross-sectional dimension than the container neck. In some embodiments, an enclosed chamber extends from the container body into the container neck (e.g., enclosed within the container, such as before thecontainer is opened). In some embodiments, the container is configured to be opened in any suitable manner. In specific embodiments, the container is configured to be opened at the container neck (e.g., by separating the head from the body, such as by unscrewing a threaded portion or by breaking the container at the container neck). In some embodiments, the container neck is configured for ease of breaking, such as through use of scoring on the neck or through use of a thinner material in the neck (e.g., relative to the body and / or head).

[0357] FIG. 12 illustrates a pair of exemplary single use containers 100 that when taken together with a composition provided herein forms an exemplary system provided herein. As demonstrated in FIG 12, a container provided herein may comprise a head 101, a neck 102, and a body 103. As illustrated the neck 102 may be narrower than the head 101 and / or body 103. In some instances, such a configuration facilitates opening the vial at the neck 102, such as by breaking the head 101 from the body 103. In some instances, an enclosed chamber 104 resides within the body 103 and may extend into the neck 102 and head 101, such that when the head 101 is separated from the body 103, such as at the neck 102, an opening or orifice is formed, from which one or more drop of a composition contained within the enclosed chamber 104 may be dispensed.

[0358] In certain embodiments, the container of a system or method described herein is an ampoule or vial, such as made from or comprising any suitable material. In some embodiments, the ampoule is a plastic ampoule or vial (e.g., wherein the ampoule or vial comprises plastic, or is substantially, such as at least 95%, comprised of plastic). In other embodiments, the ampoule or vial is a glass ampoule or vial (e.g., wherein the ampoule or vial comprises glass, or is substantially, such as at least 95%, comprised of glass).

[0359] In some embodiments, a container provided herein is sterile, such as sterile within the chamber. In certain embodiments, the chamber of the container if sterile prior to opening of an enclosed chamber of the container. In specific embodiments, the container is an ampoule or vial and the ampoule or vial is sterile (e.g., before and / or after filling the ampoule or vial with a composition provided herein).

[0360] In certain embodiments, the container comprises or is comprised of any suitable material. In specific embodiments, the container comprises (or is comprised of) a plastic. In some embodiments, the container comprises (or is comprised of) polyethylene. In specific embodiments, the container or container body or container body / neck consists essentially of polyethylene. In some embodiments, the container comprises (or is comprised of) low-density polyethylene (LDPE). In specific embodiments, the container or container body or container body / neck consists essentially of low-density polyethylene (LDPE).

[0361] In some embodiments, the container is transparent (e.g., the material from which the container is made is transparent). In certain embodiments, the container is sufficiently transparentto see the composition within, such as the amount of composition within and / or the color of the composition within.

[0362] In some embodiments, a container provided herein is configured to hold about 0.05 mL to about 5 mL of a composition within an enclosed chamber thereof (e.g., prior to opening). In specific embodiments, the container provided herein is configured to hold about 0.1 mL to about 1 mL of a composition within an enclosed chamber thereof (e g , prior to opening). In some embodiments, the container provided herein is configured to hold about 0.2 mL to about 2 mL of a composition within an enclosed chamber thereof (e.g., prior to opening). In specific embodiments, the container provided herein is configured to hold about 0.2 mL to about 1 mL of a composition within an enclosed chamber thereof (e.g., prior to opening). In more specific embodiments the container provided herein is configured to hold about 0.5 mL of a composition within an enclosed chamber thereof (e.g., prior to opening).

[0363] In certain embodiments, any container provided herein is configured to dispense a drop from an opening thereof (e.g., an opening created after opening or irreversibly opening the container). In specific embodiments, the container is configured to dispense a drop having a volume of about 0.01 mL to about 0.06 mL. In more specific embodiments, the container is configured to dispense a drop having a volume of about 0.02 mL to about 0.05 mL. In still more specific embodiments, the container is configured to dispense a drop having a volume of about 0.03 mL to about 0.04 mL.

[0364] In certain embodiments, provided herein is a system comprising a single use container (also referred to herein as a single-patient-use container) and a (e.g., ophthalmological) composition comprising aceclidine or a salt thereof. In some instances, systems provided herein facilitate the use of a dissolved aceclidine that has good stability, including at extended storage times (e.g., including under refrigerated and room temperature storage conditions).

[0365] In some embodiments, systems provided herein comprise a composition providing good stability, such as during storage (e.g., at room temperature and / or cold storage). In some instances, stability of a composition such as described herein is provided by using a system described herein. In certain instances, good stability of a composition provided herein is achieved in a system described herein even in the absence of an inert gas filling in the headspace (e.g., the void of the chamber not filled by a composition described herein) in the chamber of a container described herein.

[0366] In some embodiments, a system provided herein comprises a composition contained within a chamber of a container described herein. In certain embodiments, the remaining space with the chamber (the headspace) is filled with the gas. In some embodiments, the headspace does not need to be filled with an inert gas. In some embodiments, the headspace is not filled with an inert gas.In certain embodiments, the headspace is filled with a gas comprising oxygen, such as an air. In some embodiments, such a system surprisingly provides good long time storage stability such as described herein even in the absence of an inert gas overlay (e.g., in the headspace).

[0367] In certain embodiments, no more than 2 drops (e.g., a first drop and a second drop) of an ophthalmological composition are administered to an eye of an individual in a single day. In certain embodiments, no more than 4 drops of an ophthalmological composition are administered to an individual in a single day (e.g., no more than 2 drops to a first eye and 2 drops to a second eye). In certain instances, limiting dose to no more than 2 drops in an eye in a day provides good therapeutic effect while also reducing potential for adverse effect that can be caused by alternative product that may use or require more frequent daily dosing.

[0368] In some embodiments, a method provided herein provides a therapeutic effect, such as in treating presbyopia, increasing comfortable screen time, and / or reducing corrective lens wear time. In some embodiments, a therapeutic effect provided by a method provided herein includes improving near vision, targeted pupil size, or a combination thereof. In certain instances, a therapeutic effect provided by a method described herein extends for a desirable duration such as for 8 hours or more (e.g., 10 hours or more). In some instances, a therapeutic effect provided by a method described herein is achieved without substantial loss of distance vision, dimming, or a combination thereof.

[0369] In some embodiments, a method provided herein provides a therapeutic effect, such as in treating presbyopia. In some embodiments, a method provided herein provides a therapeutic effect, such as in improving near-vision. In some embodiments, a therapeutic effect provided by a method provided herein includes improving near vision, targeted pupil size, or a combination thereof. In certain instances, a therapeutic effect provided by a method described herein extends for a duration of 10 hours or more. In some instances, a therapeutic effect provided by a method described herein is achieved without substantial loss of distance vision, dimming, or a combination thereof.

[0370] In some embodiments, a method provided herein provides a therapeutic effect, such as in treating presbyopia. In some embodiments, a method provided herein provides a therapeutic effect, such as in improving near-vision. In some embodiments, a therapeutic effect provided by a method provided herein includes improving near vision, targeted pupil size, or a combination thereof. In certain instances, a therapeutic effect is provided within 30 minutes following administration of a composition according to a method described herein (e g., extends for a duration of 10 hours or more). In some instances, a therapeutic effect provided by a method described herein is achieved without substantial loss of distance vision, dimming, or a combination thereof.

[0371] In some embodiments, a pupil size of an eye of an individual following administration of an ophthalmological composition described herein (e.g., according to a method provided herein) to the eye is about 1.5 mm to about 2.0 mm. In some embodiments, a pupil size of about 1.5 mm to about 2.0 mm is achieved within 30 minutes of administration of the dose (e.g., 2 drops about 2 minutes apart) to the eye. In some embodiments, a pupil size of about 1.5 mm to about 2.0 mm is maintained (e g., after being achieved) for at least 8 hours following administration of the dose (e.g., 2 drops about 2 minutes apart) to the eye. In some embodiments, a pupil size of about 1.5 mm to about 2.0 mm is maintained (e.g., after being achieved) for at least 9 hours following administration of the dose (e g., 2 drops about 2 minutes apart) to the eye In some embodiments, a pupil size of about 1.5 mm to about 2.0 mm is maintained (e.g., after being achieved) for at least 10 hours following administration of the dose (e.g., 2 drops about 2 minutes apart) to the eye. In some embodiments, a pupil size of no more than 2.1 mm is maintained (e.g., after being achieved) for at least 10 hours following administration of the dose (e.g., 2 drops about 2 minutes apart) to the eye. In some embodiments, a pupil size of no more than 2.35 mm is maintained (e.g., after being achieved) for at least 10 hours following administration of the dose (e.g., 2 drops about 2 minutes apart) to the eye. In some embodiments, an effect of a method provided herein is maintained for sufficient amount of time, such as an entire workday (e.g., over a period of about 8 hours or at least about 8 hours).

[0372] In some embodiments, the reduction in pupil size is maintained in low light conditions. In certain embodiments, the reduction in pupil size is maintained indoor, such as in an office. In some instances, benefits of a composition provided herein being administered to the eye can be maintained even in conditions such as low light in an office, etc. where pupil dilation would normally be expected to occur. In some embodiments, low light condition is less than about 2000 candela per square meter (cd / m2). In some embodiments, low light condition is less than about 200 cd / m2. In some embodiments, low light condition is less than about 20 cd / m2.

[0373] In some embodiments, near vision of an eye of an individual following administration of an ophthalmological composition described herein (e.g., according to a method provided herein) to the eye is improved by at least 2 lines. In some embodiments, the near vision is improved by 3 lines or more in the eye.

[0374] In some embodiments, near vision improvement (e.g., at least 2 lines) is achieved within 30 minutes of administration of the dose (e.g., 2 drops about 2 minutes apart) to the eye. In some embodiments, near vision improvement (e.g., at least 3 lines) is achieved within 30 minutes of administration of the dose (e.g., 2 drops about 2 minutes apart) to the eye. In some embodiments, near vision improvement (e.g., at least 2 lines) is achieved within 60 minutes of administration of the dose (e.g., 2 drops about 2 minutes apart) to the eye. In some embodiments, near visionimprovement (e.g., at least 3 lines) is maintained (e.g., after being achieved) for at least 8 hours following administration of the dose (e g., 2 drops about 2 minutes apart) to the eye. In some embodiments, near vision improvement (e g., at least 2 lines) is maintained (e g., after being achieved) for at least 10 hours following administration of the dose (e.g., 2 drops about 2 minutes apart) to the eye. In some embodiments, near vision improvement (e.g., at least 3 lines) is maintained (e.g., after being achieved) for at least 9 hours following administration of the dose (e.g., 2 drops about 2 minutes apart) to the eye. In some embodiments, near vision improvement (e.g., at least 3 lines) is maintained (e.g., after being achieved) for at least 10 hours following administration of the dose (e.g., 2 drops about 2 minutes apart) to the eye.

[0375] In some embodiments, a method of treating presbyopia provided herein is a method of treating moderate presbyopia (e.g., the method comprising administrating a composition provided herein, such as using any method provided herein). In some instances, an individual with moderate presbyopia has 20 / 50 to 20 / 80 vision at 40 cm. In some embodiments, the method of treating moderate presbyopia in an individual provides at least 3-line improvement in near vision (e.g., at 40 cm) in the individual. In some embodiments, the method of treating moderate presbyopia in an individual provides at least 4-line improvement in near vision (e.g., at 40 cm) in the individual.

[0376] In some embodiments, a method of treating presbyopia provided herein is a method of treating advanced presbyopia (e.g., the method comprising administrating a composition provided herein, such as using any method provided herein). In some instances, an individual with advanced presbyopia has > 20 / 80 vision (i.e., worse than 20 / 80 vision) at 40 cm. In some embodiments, the method of treating advanced presbyopia in an individual provides at least 4-line improvement in near vision (e.g., at 40 cm) in the individual. In some embodiments, the method of treating advanced presbyopia in an individual provides at least 5-line improvement in near vision (e.g., at 40 cm) in the individual. In some embodiments, the method of treating advanced presbyopia in an individual provides at least 6-line improvement in near vision (e.g., at 40 cm) in the individual.

[0377] In some embodiments, a method of treating presbyopia provided herein (e.g., a method of treating moderate or advanced presbyopia) in an individual provides a vision of 20 / 32 or better in the individual (e.g., at 40 cm). FIG. 9 illustrates near vision improvement in individuals with moderate and advanced presbyopia.

[0378] In some embodiments, a method of treating presbyopia provided herein is a method of treating presbyopia in an individual needing greater than a 3 -line improvement in near vision (e.g., at 40 cm). In some embodiments, a method of treating presbyopia provided herein is a method of treating presbyopia in an individual needing greater than a 4-line improvement in near vision (e g., at 40 cm). In some embodiments, a method of treating presbyopia provided herein is a method of treating presbyopia in an individual needing greater than a 5-line improvement in near vision (e g.,at 40 cm). In some embodiments, a method of treating presbyopia provided herein is a method of treating presbyopia in an individual needing greater than a 6-line improvement in near vision (e g., at 40 cm).

[0379] In some embodiments, the method of treating presbyopia in an individual provides at least 3-line improvement in near vision (e.g., at 40 cm) in the individual. In some embodiments, the method of treating presbyopia in an individual provides at least 4-line improvement in near vision (e.g., at 40 cm) in the individual. In some embodiments, the method of treating presbyopia in an individual provides at least 5-line improvement in near vision (e.g., at 40 cm) in the individual. In some embodiments, the method of treating presbyopia in an individual provides at least 6-line improvement in near vision (e.g., at 40 cm) in the individual.

[0380] FIG. 11 illustrates near vision improvement of at least 4 lines and up to 8 lines in individuals with presbyopia.

[0381] In some instances, near vision improvement is determined in any suitable manner. In specific instances, near vision improvement is determined by (e.g., high contrast) best corrected distance visual acuity (BCDVA) at 40 cm, such as High Contrast BCDVA. In some instances, BCDVA protocols are understood by the skilled artisan, such as provided in DIAMOND BCdVA & Refraction Guideline Version 1.0_Final_01 / 12 / 2016. In some embodiments, lines or letters of improvement for near vision describes an improvement in visual acuity, such as is determined by a best corrected distance visual acuity (BCDVA) test at 40 centimeters.

[0382] In some embodiments, no loss of 1 line or more (e.g., greater than or equal to 5 letters) of distance vision is achieved following administration of an ophthalmological composition described herein (e.g., according to a method provided herein, such as 2 drops about 2 minutes apart) to the eye (e.g., within 8 or 10 hours).

[0383] In some embodiments, a method provided herein provides improvement of at least 2 letters of distance vision (e.g., following administration of an ophthalmological composition described herein (e.g., according to a method provided herein, such as 2 drops about 2 minutes apart) to the eye (e.g., for 8 or 10 hours)). In some embodiments, a method provided herein provides improvement of 2-4 letters of distance vision (e.g., following administration of an ophthalmological composition described herein (e.g., according to a method provided herein, such as 2 drops about 2 minutes apart) to the eye (e.g., for 8 or 10 hours)). In some embodiments, a method provided herein provides improvement of at least 3-4 letters of distance vision (e g., following administration of an ophthalmological composition described herein (e.g., according to a method provided herein, such as 2 drops about 2 minutes apart) to the eye (e.g., for 8 or 10 hours)). In some embodiments, a method provided herein provides improvement of at least 1 line of distance vision (e.g., following administration of an ophthalmological composition describedherein (e.g., according to a method provided herein, such as 2 drops about 2 minutes apart) to the eye (e.g., for 8 or 10 hours)). In some embodiments, the distant vision is improved for at least 8 hours In some embodiments, the distant vision is improved for at least 10 hours. In some embodiments, the distant vision is improved within 30 minutes.

[0384] In some instances, distance vision loss or improvement is determined in any suitable manner In specific instances, distance vision loss is or improvement determined by best corrected distance visual acuity (BCDVA) at 4 meters. In some embodiments, lines or letters of improvement for distance vision describes an improvement in visual acuity, such as is determined by a best corrected distance visual acuity (BCDVA) test at 4 meters.

[0385] In some embodiments, the ophthalmological composition can be administered to the eye for any suitable or desirable duration. In certain embodiments, dosing according to a method provided herein can last at least 6 weeks. In certain embodiments, dosing according to a method provided herein can last at least 24 weeks.

[0386] In certain embodiments, an ophthalmological composition provided herein (e.g., in any system described herein) comprises a miotic agent (e.g., aceclidine) or a salt thereof. In some embodiments, the miotic is a pupil selective miotic, such as aceclidine or a salt thereof. In certain embodiments, an ophthalmological composition provided herein (e.g., in any system described herein) comprises aceclidine or a salt thereof. In specific embodiments, the ophthalmological composition comprises (or is formulated with) aceclidine or a salt thereof in a concentration of about 1 wt. % to about 2 wt. %. In specific embodiments, the ophthalmological composition is formulated with an aceclidine salt, such as aceclidine hydrochloride, in an amount of about 1.6 wt. % to about 1.8 wt. % (e.g., about 1.75 wt. %). In certain embodiments, the ophthalmological composition comprises aceclidine in an amount of about 1.4 wt. % to about 1.5 wt. %, such as about 1.44 wt. % to about 1.46 wt. % (e.g., based on free base concentration of aceclidine).

[0387] In some embodiments, an ophthalmological composition provided herein is a pupil selective miotic composition (e.g., comprising a pupil selective miotic such as aceclidine or a salt thereof). In some embodiments, a pupil selective miotic composition or a pupil selective miotic selectively (but not necessarily exclusively) constricts the pupil relative to the ciliary muscle. In some instances, selectivity includes greater activity on the pupil relative to the ciliary such as at least 1.2x, at least 1.5x, at least 2x, at least 3x, at least 5x, at least lOx, at least 20x, or the like (e.g., as determined by the concentration required to produce 50% of a maximum ciliary response (longitudinal or circular) divided by a concentration required to reduce 50% maximum iris sphincter response).

[0388] In some instances, a selective miotic reduces risk of adverse effects. In certain instances, this may allow more efficacy of a longer therapies, particularly in individuals who have previously had ocular surgeries such as an ocular surgery described herein

[0389] Provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmological composition to an eye of the individual, and the eye of the individual having previously undergone ocular surgery.

[0390] Also, provided in some embodiments herein is a method of treating presbyopia in an individual, the method comprising administering aceclidine or a salt thereof to an eye of the individual, and the eye of the individual having previously undergone ocular surgery.

[0391] In some embodiments, an individual treated by any method provided herein has previously undergone ocular surgery and that ocular surgery is a post-refractive surgery (e.g., in an eye treated according to a method provided herein).

[0392] In some embodiments, an individual treated by any method provided herein has previously undergone ocular surgery and that ocular surgery is a laser assisted in situ keratomileusis (LASIK) surgery (e.g., in an eye treated according to a method provided herein).

[0393] In some embodiments, an individual treated by any method provided herein has previously undergone ocular surgery and that ocular surgery is a photorefractive keratectomy (PRK) surgery (e.g., in an eye treated according to a method provided herein).

[0394] In some embodiments, an individual treated by any method provided herein is a pseudophakia individual.

[0395] In some embodiments, an individual treated by any method provided herein has previously undergone ocular surgery and that ocular surgery is a post cataract-intraocular lens (IOL) surgery (e g., in an eye treated according to a method provided herein).

[0396] In some embodiments, an individual treated by any method provided herein has previously undergone ocular surgery and that ocular surgery is a post-installation of fake lens surgery (e.g., in an eye treated according to a method provided herein).

[0397] In certain instances, an individual treated according to a method provided herein prior to surgery wore a corrective contact lens or corrective glasses (e.g., bifocals), and after surgery and administration of the ophthalmological composition, the individual does not need to (or does not) wear the corrective contact lens or corrective glasses (e g., bifocals).

[0398] In certain instances, an individual treated according to a method provided herein prior to surgery wore a corrective contact lens or corrective glasses (e.g., bifocals), and after surgery and administration of the ophthalmological composition, the individual has less of a need to wear (or can wear a lower power of) the corrective contact lens or corrective glasses (e.g., bifocals).

[0399] In some embodiments, provided herein is a method of treating presbyopia in an individual wearing a corrective lens (e.g., contact lens). In some embodiments, provided herein is a method of treating presbyopia in an individual wearing a contact lens. In certain embodiments, the method comprises administering (e.g., topically) one or more drop of an ophthalmic composition comprising aceclidine to an eye (e.g., an ocular surface thereof) of the individual described herein. In specific embodiments, the method comprises removing the contact lens from the eye before administration of an ophthalmological composition provided herein to the eye. In more specific embodiments, the method comprises reinserting the contact lens onto the eye (e.g., about 10 minutes or more) after administration of the ophthalmological composition

[0400] In some embodiments, any ophthalmological composition provided herein comprises an alpha-2 agonist (e.g., brimonidine) or a salt thereof. In some embodiments, any ophthalmological composition provided herein comprises brimonidine or a salt thereof. In some embodiments, the ophthalmological composition comprises (or is formulated with) brimonidine or a salt thereof in a concentration of about 0.05 wt. % to about 0.15 wt. %. In some embodiments, the ophthalmological composition comprises (or is formulated with) brimonidine or a salt thereof in a concentration of 0.07 wt. % to about 0.15 wt. %. In specific embodiments, the ophthalmological composition comprises (or is formulated with) brimonidine or a salt thereof in a concentration of 0.07 wt. % to about 0.1 wt. %. In more specific embodiments, the ophthalmological composition comprises (or is formulated with) brimonidine or a salt thereof in a concentration of about 0.08 wt. %. In some embodiments, the ophthalmological composition is formulated with a brimonidine salt, such as brimonidine tartrate, in an amount of 0.7 wt. % to about 0.1 wt. % (e.g., about 0.08 wt. %). In certain embodiments, the ophthalmological composition comprises brimonidine in an amount of about 0.4 wt. % to about 1 wt. %, such as about 0.5 wt. % to about 0.6 wt. % (e g., based on free base concentration of brimonidine).

[0401] In some embodiments, a composition provided herein comprises aceclidine or a salt thereof and brimonidine or a salt thereof. In some embodiments, a composition provided herein comprises aceclidine hydrochloride in a concentration of about 1.6 wt. % to about 1.8 wt. % and brimonidine tartrate in a concentration of 0.07 wt. % to about 0.15 wt. %. In specific embodiments, a composition provided herein comprises aceclidine hydrochloride in a concentration of about 1.75 wt. % and brimonidine tartrate in a concentration of about 0.08 wt. %.

[0402] In some embodiments, a composition provided herein comprises aceclidine in an amount of about 1.4 wt. % to about 1.5 wt. %, such as about 1.44 wt. % to about 1.46 wt. % (e.g., based on free base concentration of aceclidine) and brimonidine in an amount of about 0.4 wt. % to about 1 wt. %, such as about 0.5 wt. % to about 0.6 wt. % (e.g., based on free base concentration of brimonidine).

[0403] In some embodiments, an agent (e.g., an active agent such as aceclidine and / or brimonidine) provided herein may comprise one or more of any suitable isotope, such as a hydrogen isotope (e.g., deuterium) and / or a carbon isotope.

[0404] In certain embodiments, a composition provided herein has a pH of 6 or less, such as a pH of about 4 to about 6. In some embodiments, the composition has a pH of about 4.5 to about 6. In some embodiments, the composition has a pH of about 4.5 to about 5.5. In some embodiments, the composition has a pH of about 5.0 to about 5.5. In some instances, use of a single use container, such as provided herein, in combination with a pH described herein allows the use of aceclidine in an aqueous composition that has good stability, including room temperature (e g., up to 25 °C) stability.

[0405] Provided in certain embodiments herein is an aqueous ophthalmic composition comprising an aceclidine or a salt thereof and a pH of about 4 to about 6 (e g., about 4.5 to about 5.5). In specific embodiments, the composition comprises a buffer. FIGs. 1A-1D, FIGs. 2A-2D, FIGs. 3A-3D, illustrate stability (% aceclidine relative to initial aceclidine) of an exemplary aqueous aceclidine composition (solution) provided herein when stored at refrigerated (e.g., 2 °C to 8 °C), room temperature (e.g., up to 25 °C), and elevated (up to 40 °C). Specifically, FIG. 1A, FIG. 2A, and FIG. 3A, demonstrate excellent stability for compositions having a pH of 5, in a largely buffer independent manner. Excellent stability is demonstrated at buffer concentrations of 0.06%, 0.08%, and 0.1% (FIG. 1A, FIG. 2A, and FIG. 3A, respectively). All three formulations demonstrate > 90% stability for aqueous aceclidine compositions (solutions) when stored for at least 6 months at room temperature and at least 18 months when refrigerated. All three formulations also demonstrate > 90% stability when stored for at least 1 month at elevated temperature (40 °C).

[0406] Similarly, FIG. IB, FIG. 2B, and FIG. 3B, demonstrate excellent stability for compositions having a pH of 5.5, in a largely buffer independent manner. Excellent stability is demonstrated at buffer concentrations of 0.06%, 0.08%, and 0.1% (FIG. IB, FIG. 2B, and FIG. 3B, respectively). All three formulations demonstrate > 90% stability for aqueous aceclidine compositions (solutions) when stored for at least 6 months at room temperature and at least 18 months when refrigerated. All three formulations also demonstrate > 90% stability when stored for at least 1 month at elevated temperature (40 °C).

[0407] FIG. 1C, FIG. 2C, and FIG. 3C, demonstrate good stability (slightly less than at pH of 5 or 5.5) for compositions having a pH of 6, in a largely buffer independent manner. Good stability is demonstrated at buffer concentrations of 0.06%, 0.08%, and 0.1% (FIG. 1C, FIG. 2C, and FIG. 3C, respectively). All three formulations demonstrate > 90% stability for aqueous aceclidine compositions (solutions) when stored for at least 6 months at room temperature and at least 18months when refrigerated. All three formulations also demonstrate > 90% stability when stored for at least 1 month at elevated temperature (40 °C).

[0408] FIG. ID, FIG. 2D, and FIG. 3D, demonstrate reduced stability (relative to pH of 5, 5.5, or 6) for compositions having a pH of 6.5, in a largely buffer independent manner. Stability is demonstrated at buffer concentrations of 0.06%, 0.08%, and 0.1% (FIG. ID, FIG. 2D, and FIG. 3D, respectively). All three formulations demonstrate approximately 90% stability or less for aqueous aceclidine compositions (solutions) when stored for 6 months at room temperature. All three formulations also demonstrate < 90% stability when stored for at least 1 month at elevated temperature (40 °C).

[0409] Moreover, FIG. 4A and FIG. 4B demonstrate excellent stability in such aqueous aceclidine compositions (solutions) in a substantially preservative independent manner. Similarly, FIG. 4C, FIG. 4D, and FIG. 4E demonstrate excellent stability in such aqueous aceclidine compositions (solutions) in a substantially viscosity agent (HPMC) independent manner. Surprisingly, varying certain components, such as buffer concentration or adding a preservative, was not observed to substantially no effect on aceclidine stability of aceclidine compositions described herein.

[0410] In some instances, compositions provided herein (e.g., comprising aceclidine and a pH of 4.5 to 6) have stability profiles that are acceptable for manufacture, shipment, and / or (e.g., longterm) storage of aceclidine.[4H] In some embodiments, composition comprises or is formulated with salt (e.g., NaCl), an acid (e.g., HC1), and / or a base (e.g., NaOH), such as to adjust osmolarity or pH.

[0412] In some embodiments, any suitable salt can be used in an agent (e.g., aceclidine and brimonidine) provided herein. In some embodiments, the salt is a hydrochloride, hydrobromide, sulfate, acetate, phosphate or diphosphate, chloride, bromide, maleate, citrate, mesylate, nitrate, tartrate, or gluconate.

[0413] In some embodiments, methods described herein provide stability (e.g., chemical stability or physical stability) of the ophthalmological drug in the composition. In some embodiments, the compositions described herein provide stability (e.g., chemical stability or physical stability) of the ophthalmological drug in the composition. In some embodiments, the systems described herein provide stability (e.g., chemical stability or physical stability) of the ophthalmological drug in the composition.

[0414] In some embodiments, chemical stability comprises having at least 80% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 3 months. In some embodiments, chemical stability comprises having at least 80% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 6 months. In some embodiments, chemical stabilitycomprises having at least 80% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 9 months. In some embodiments, chemical stability comprises having at least 80% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 12 months. In some embodiments, chemical stability comprises having at least 80% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 18 months.

[0415] In some embodiments, chemical stability comprises having at least 90% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 3 months. In some embodiments, chemical stability comprises having at least 90% of the ophthalmological drug (e g., aceclidine) remaining in a composition after 6 months. In some embodiments, chemical stability comprises having at least 90% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 9 months. In some embodiments, chemical stability comprises having at least 90% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 12 months. In some embodiments, chemical stability comprises having at least 90% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 18 months.

[0416] In some embodiments, chemical stability comprises having at least 95% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 3 months. In some embodiments, chemical stability comprises having at least 95% of the ophthalmological drug (e g., aceclidine) remaining in a composition after 6 months. In some embodiments, chemical stability comprises having at least 95% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 9 months. In some embodiments, chemical stability comprises having at least 95% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 12 months. In some embodiments, chemical stability comprises having at least 95% of the ophthalmological drug (e.g., aceclidine) remaining in a composition after 18 months.

[0417] In some embodiments, chemical stability comprises having at least 80% of the ophthalmological drug (e.g., aceclidine) present in the composition at the time of administration relative to the initial amount of the ophthalmological drug. In some embodiments, chemical stability comprises having at least 85% of the ophthalmological drug (e.g., aceclidine) present in the composition at the time of administration relative to the initial amount of the ophthalmological drug. In some embodiments, chemical stability comprises having at least 90% of the ophthalmological drug (e.g., aceclidine) present in the composition at the time of administration relative to the initial amount of the ophthalmological drug. In some embodiments, chemical stability comprises having at least 94% of the ophthalmological drug (e.g., aceclidine) present in the composition at the time of administration relative to the initial amount of the ophthalmological drug. In some embodiments, chemical stability comprises having at least 96% of the ophthalmological drug (e.g., aceclidine) present in the composition at the time of administrationrelative to the initial amount of the ophthalmological drug. In some embodiments, chemical stability comprises having at least 97% of the ophthalmological drug (e.g., aceclidine) present in the composition at the time of administration relative to the initial amount of the ophthalmological drug. In some embodiments, chemical stability comprises having at least 98% of the ophthalmological drug (e.g., aceclidine) present in the composition at the time of administration relative to the initial amount of the ophthalmological drug. In some embodiments, chemical stability comprises having at least 99% of the ophthalmological drug (e.g., aceclidine) present in the composition at the time of administration relative to the initial amount of the ophthalmological drug.

[0418] In some embodiments, an initial amount of the ophthalmological drug is the amount of the ophthalmological drug originally present in the composition (e.g., at time of manufacture or at time of preparation). In some embodiments, an initial amount of the ophthalmological drug is the amount of the ophthalmological drug at the time of manufacture of the composition. In some embodiments, an initial amount of the ophthalmological drug is the amount of the ophthalmological drug at the time of preparing the composition. In some embodiments, an initial amount of the ophthalmological drug is the amount of the ophthalmological drug in the composition prior to storing the composition.

[0419] In some instances, a system provided herein allows for the room temperature (e.g., up to 25 °C) storage (e.g., by an end user) for (e.g., up to) 3 months. In specific instances, a system provided herein allows for the room temperature (e.g., up to 25 °C) storage (e.g., by an end user) for (e.g., up to) 6 months.

[0420] In some embodiments, a system provided herein allows for refrigerated storage (e.g., at 2 °C to 8 °C) for at least 8 months. In some embodiments, a system provided herein allows for refrigerated storage (e.g., at 2 °C to 8 °C) for at least 10 months. In some embodiments, a system provided herein allows for refrigerated storage (e.g., at 2 °C to 8 °C) for at least 11 months. In some embodiments, a system provided herein allows for refrigerated storage (e.g., at 2 °C to 8 °C) for at least 12 months. In some embodiments, a system provided herein allows for refrigerated storage (e.g., at 2 °C to 8 °C) for at least 14 months. In some embodiments, a system provided herein allows for refrigerated storage (e.g., at 2 °C to 8 °C) for at least 16 months. In some embodiments, a system provided herein allows for refrigerated storage (e.g., at 2 °C to 8 °C) for at least 18 months. In some embodiments, a system provided herein allows for refrigerated storage (e.g., at 2 °C to 8 °C) for at least 20 months. In some embodiments, a system provided herein allows for refrigerated storage (e.g., at 2 °C to 8 °C) for at least 22 months. In some embodiments, a system provided herein allows for refrigerated storage (e.g., at 2 °C to 8 °C) for at least 24 months. For example, the system can facilitate retention of at least 90%, at least 95%, at least96%, at least 97%, at least 98%, or at least 99% of the initial amount of aceclidine (e.g., upon initial formulation or immediately prior to storage) following the period of storage.

[0421] In some embodiments, a system provided herein allows for room temperature storage as disclosed herein for at least 2 months. In some embodiments, a system provided herein allows for room temperature storage as disclosed herein for at least 3 months. In some embodiments, a system provided herein allows for room temperature storage as disclosed herein for at least 4 months. In some embodiments, a system provided herein allows for room temperature storage as disclosed herein for at least 5 months. In some embodiments, a system provided herein allows for room temperature storage as disclosed herein for at least 6 months. For example, the system can facilitate retention of at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the initial amount of aceclidine (e.g., upon initial formulation or immediately prior to storage) following the period of storage.

[0422] In some embodiments, a system provided herein allows for storage at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 5 days. In some embodiments, a system provided herein allows for storage at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 6 days. In some embodiments, a system provided herein allows for storage at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 7 days. In some embodiments, a system provided herein allows for storage at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 8 days. In some embodiments, a system provided herein allows for storage at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 9 days. In some embodiments, a system provided herein allows for storage at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 10 days. For example, the system can facilitate retention of at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the initial amount of aceclidine (e.g., upon initial formulation or immediately prior to storage) following the period of storage.

[0423] In some embodiments, a system provided herein allows for storage at (i) refrigerated temperature (e.g., at 2 °C to 8 °C), (ii) room temperature as disclosed herein (e.g., up to 25°C, 15- 25°C, 20-25°C, or 23-25°C), and (iii) elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C). For example, the system can facilitate retention of at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the initial amount of aceclidine (e.g., upon initial formulation or immediately prior to storage) following the combined periods of storage at refrigerated temperature, room temperature, and elevated temperature.

[0424] In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 1 month, and (iii) elevated temperature for at least 8 days. In some embodiments, the system provided herein allows forstorage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 2 months, and (iii) elevated temperature for at least 8 days. In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 4 months, and (iii) elevated temperature for at least 8 days. In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 6 months, and (iii) elevated temperature for at least 8 days.

[0425] In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 1 month, and (iii) elevated temperature for at least 4 days. In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 2 months, and (iii) elevated temperature for at least 4 days. In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 4 months, and (iii) elevated temperature for at least 4 days. In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 6 months, and (iii) elevated temperature for at least 4 days.

[0426] In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 1 month, and (iii) elevated temperature for at least 8 days. In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 2 months, and (iii) elevated temperature for at least 8 days. In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 4 months, and (iii) elevated temperature for at least 8 days. In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 6 months, and (iii) elevated temperature for at least 8 days.

[0427] In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 1 month, and (iii) elevated temperature for at least 4 days. In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 2 months, and (iii) elevated temperature for at least 4 days. In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 4 months, and (iii) elevated temperature for at least 4 days. In some embodiments, the system provided herein allows for storage at (i) refrigerated temperature for atleast 18 months, (ii) room temperature for at least 6 months, and (iii) elevated temperature for at least 4 days.

[0428] In certain embodiments, a method provided herein uses a composition that has been stored at room temperature prior to use (e.g., administration to the eye of an individual for treating presbyopia). In some embodiments, the method comprises storing the composition at room temperature (e g., up to 25 °C) for up to 6 months In some instances, such a recitation means that the composition is stored for any time of less than 6 months. In other instances, such a recitation means that the composition is stored for any point of time up to 6 months and that the composition is suitable for use for any length of time up to 6 months of storage at room temperature (e g., up to 25°C). In some embodiments, the method comprises storing the composition at room temperature (e g., up to 25 °C) for up to 3 months. In some instances, such a recitation means that the composition is stored for any time of less than 3 months. In other instances, such a recitation means that the composition is stored for any point of time up to 3 months and that the composition is suitable for use for any length of time up to 3 months of storage at room temperature (e.g., up to 25°C). In some instances, a composition suitable for use is a composition that retains at least 90% (or other suitable amount provided herein) of the initial amount of aceclidine in the composition.

[0429] In certain embodiments, a method provided herein uses a composition that has been stored at 2 °C to 8 °C prior to use (e.g., administration to the eye of an individual for treating presbyopia). In some embodiments, the method comprises storing the composition at 2 °C to 8 °C for up to 12- 18 months (e.g., up to 12 months or up to 18 months). In some instances, such a recitation means that the composition is stored for any time of less than 12-18 months. In other instances, such a recitation means that the composition is stored for any point of time up to 12-18 months and that the composition is suitable for use for any length of time up to 12-18 months of storage at 2 °C to 8 °C. In some embodiments, the method comprises storing the composition at 2 °C to 8 °C for up to 12-18 months. In some instances, such a recitation means that the composition is stored for any time of less than 12-18 months. In other instances, such a recitation means that the composition is stored for any point of time up to 12-18 months and that the composition is suitable for use for any length of time up to 12-18 months of storage at room temperature 2 °C to 8 °C.

[0430] In some instances, a composition suitable for use is a composition that retains at least 95% (or other suitable amount provided herein) of the initial amount of aceclidine in the composition.

[0431] In some instances, end users (individual) may use a composition provided herein and store the composition at room temperature following cold chain storage by manufacturer and / or pharmacist. As such, in some instances, storage of a composition provided herein retains at least 95% of initial amount of aceclidine after storage at 2 °C to 8 °C and at least 90% of initial amount(e.g., initial amount being the amount present in the composition before cold and room temperature storage) of aceclidine after room temperature storage and upon use by the individual (end user).

[0432] In certain embodiments, a method provided herein uses a composition that has been stored at a temperature of above 25 °C, up to 40 °C, prior to use (e.g., administration to the eye of an individual for treating presbyopia). In some embodiments, the method comprises storing the composition at temperature of above 25 °C, up to 40 °C, for up to 1-2 months (e.g., up to 8 days). In some instances, such a recitation means that the composition is stored for any time of less or equal to than 1-2 months (e.g., less than or equal to 8 days). In other instances, such a recitation means that the composition is stored for any point of time up to 1-2 months (e.g., up to 8 days) and that the composition is suitable for use for any length of time up to 1-2 months (e.g., up to 8 days) of storage at temperature of above 25 °C, up to 40 °C.

[0433] In some instances, during shipping, for example, a composition provided herein is subject to temperatures above room temperature. In certain instances, it is advantageous for a composition provided herein to be stable for at least short periods of time at elevated temperature. In some instances, storage of a composition provided herein at a temperature of above 25 °C, up to 40 °C retains at least 90% (e.g., at least 95% or other amount described herein) aceclidine relative to an initial amount (e g., prior to storage at a temperature of above 25 °C, up to 40 °C).

[0434] In some embodiments, a composition described herein comprises at least 90% (e g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of at least a room temperature (e.g., about 15 °C to about 30°C, about 20 °C to about 25 °C) for at least about two months. In some embodiments, a composition described herein comprises at least 95% (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e.g., aceclidine) after being stored at a temperature of at least a room temperature for at least about two months. In some embodiments, a composition described herein comprises 95% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of at least a room temperature for at least about two months. In some embodiments, a composition described herein comprises 96% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of at least a room temperature for at least about two months. In some embodiments, a composition described herein comprises 97% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of at least a room temperature for at least about two months. In some embodiments, a composition described herein comprises 98% or more (e.g., compared to a baseline measurement) of anophthalmological drug described herein (e.g., aceclidine) after being stored at a temperature of at least a room temperature for at least about two months. In some embodiments, a composition described herein comprises 99% or more (e g , compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of at least a room temperature for at least about two months.

[0435] In some embodiments, a composition described herein comprises at least 85% (e g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of about 40 °C for about one month or more or two months or more. In some embodiments, a composition described herein comprises at least 90% (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of about 40 °C for about one month or more or two months or more. In some embodiments, a composition described herein comprises at least 95% (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of about 40 °C for about one month or more or two months or more. In some embodiments, a composition described herein comprises 85% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of about 40 °C for about one month or more or two months or more. In some embodiments, a composition described herein comprises 90% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of about 40 °C for about one month or more or two months or more. In some embodiments, a composition described herein comprises 95% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of about 40 °C for about one month or more or two months or more.

[0436] In some embodiments, a composition described herein comprises at least 90% (e g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of at least a room temperature (e.g., about 15 °C to about 30 °C, about 20 °C to about 25 °C) for about one month or more, two months or more, about three months or more, about four months or more, about five months or more, or about six months or more. In some embodiments, a composition described herein comprises at least 95% (e g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of at least a room temperature for about one month or more, about two months or more, about three months or more, about four months or more, about five months or more, or about six months or more. In some embodiments, a composition described herein comprises 95% or more (e.g., compared to a baseline measurement) of anophthalmological drug described herein (e.g., aceclidine) after being stored at a temperature of at least a room temperature for about one month or more, about two months or more, about three months or more, about four months or more, about five months or more, or about six months or more. In some embodiments, a composition described herein comprises 96% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e.g., aceclidine) after being stored at a temperature of at least a room temperature for about one month or more, about two months or more, about three months or more, about four months or more, about five months or more, or about six months or more. In some embodiments, a composition described herein comprises 97% or more (e g , compared to a baseline measurement) of an ophthalmological drug described herein (e.g., aceclidine) after being stored at a temperature of at least a room temperature for, about one month or more, about two months or more, about three months or more, about four months or more, about five months or more, or about six months or more. In some embodiments, a composition described herein comprises 98% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of at least a room temperature for about one month or more, about two months or more, about three months or more, about four months or more, about five months or more, or about six months or more. In some embodiments, a composition described herein comprises 99% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of at least a room temperature for about one month or more, about two months or more, about three months or more, about four months or more, about five months or more, or about six months or more.

[0437] In some embodiments, a composition described herein comprises at least 90% (e g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of at least 0 °C for at least 1 month. In some embodiments, a composition described herein comprises at least 95% (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e.g., aceclidine) after being stored at a temperature of at least 0 °C for at least 1 month. In some embodiments, a composition described herein comprises 95% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e g., aceclidine) after being stored at a temperature of at least 0 °C for at least 1 month. In some embodiments, a composition described herein comprises 96% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e.g., aceclidine) after being stored at a temperature of at least 0 °C for at least 1 month. In some embodiments, a composition described herein comprises 97% or more (e g., compared to a baseline measurement) of an ophthalmological drug described herein (e.g., aceclidine) after beingstored at a temperature of at least 0 °C for at least 1 month. In some embodiments, a composition described herein comprises 98% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e.g., aceclidine) after being stored at a temperature of at least 0 °C for at least 1 month. In some embodiments, a composition described herein comprises 99% or more (e.g., compared to a baseline measurement) of an ophthalmological drug described herein (e.g., aceclidine) after being stored at a temperature of at least 0 °C for at least 1 month

[0438] In some embodiments, storage temperature and / or storage time, as described elsewhere herein, affect the chemical stability of an ophthalmological drug in a composition.

[0439] In some embodiments, chemical stability of the ophthalmological drug in a composition is affected at a certain pH of the composition. In some embodiments, chemical stability of the ophthalmological drug in a composition is greater in a composition having a lower pH, such as relative to an otherwise similar composition having a higher pH. In some embodiments, a composition comprising aceclidine having a pH of 5 is more stable after storing relative to an otherwise similar composition having a pH of 6, where stability is amount of aceclidine present in the composition at the end of a storing period relative to onset of storing. In some embodiments, a composition comprising aceclidine having a pH of 5 is more stable after storing at 5 degrees Celsius for 18 months relative to an otherwise similar composition having a pH of 6, where stability is amount of aceclidine present in the composition at the end of a storing period relative to onset of storing. In some embodiments, a composition comprising aceclidine having a pH of 5 is more stable after storing at 25 degrees Celsius for 6 months relative to an otherwise similar composition having a pH of 6, where stability is amount of aceclidine present in the composition at the end of a storing period relative to onset of storing.

[0440] In some embodiments, a composition having a pH of about 4.5 to about 6 has a greater stability of the ophthalmological drug relative to an otherwise similar composition having a pH of more than about 6. In some embodiments, a composition having a pH of about 4.5 to about 5.5 has a greater stability of the ophthalmological drug relative to an otherwise similar composition having a pH of more than about 6. In some embodiments, a composition having a pH of about 5 to about 6 has a greater stability of the ophthalmological drug relative to an otherwise similar composition having a pH of more than about 6. In some embodiments, a composition comprising the ophthalmological drug has a stability of the ophthalmological drug greater than 90% at a pH between about 4.5 and 6 (e.g., pH about 5 to about 6 or pH about 4.5 to about 5.5) after storing for up to 6 months at a room temperature. In some embodiments, a composition comprising the ophthalmological drug has a stability of the ophthalmological drug less than 90% at a pH between above 6 (e.g., pH above 6.5) after storing for up to 6 months at a room temperature.

[0441] Aceclidine compositions described herein, such as aceclidine compositions having a pH of less than 6 (e g., about 5.0 or about 5.5), can have improved aceclidine stability profiles at room temperature (or higher temperatures) for an extended period of time, such as for two months or more, three months or more, four months or more, five months or more, or six months or more, such as compared to aceclidine compositions having a pH of 6 or more. Moreover, in some embodiments, surprisingly, changing certain parameters, such as buffer concentration or adding a preservative, has little to no effect on aceclidine stability in compositions described herein stored under certain conditions.

[0442] Moreover, while in some embodiments aceclidine compositions disclosed herein (e g., having a pH of 5.0 and 5.5) have similar stability profiles at 5 °C and room temperature over the same periods of time, aceclidine compositions having a buffer concentration (e.g., 0.06%) in some embodiments provide further improvements in aceclidine stability profile at higher temperatures, such as at about 40 °C.

[0443] Data provided herein demonstrates, in some embodiments, that compositions described herein (e.g., comprising aceclidine and a pH of 5 or 5.5), such as aqueous aceclidine compositions described herein, have aceclidine stability profiles that are acceptable for manufacture, shipment, and / or (e.g., long-term) storage of aceclidine at room temperature (or higher temperatures).

[0444] Furthermore, data provided herein demonstrates, in some embodiments, that aqueous compositions comprising aceclidine described herein can be made (and shipped) and stored (e g., at room temperature or higher) with little to no degradation of aceclidine.

[0445] In some aspects, provided herein is a method of treating presbyopia in an individual, the method comprising administering an ophthalmologically acceptable composition to an eye of the individual, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, and the composition having a room temperature when administered to the eye of the individual.

[0446] In some embodiments, the method of treating presbyopia in an individual comprises administering an ophthalmologically acceptable composition to an eye of the individual, the composition comprising aceclidine having a pH of about 4.5 to about 6 and the composition having a room temperature when administered to the eye of the individual.

[0447] In some aspects, provided herein is a composition for use in treating presbyopia in an individual, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, and the composition having a room temperature when administered to an eye of the individual.

[0448] In some aspect, provided herein is a method of treating presbyopia in an individual, the method comprising administering to the individual an ophthalmologically acceptable composition to an eye of the individual, the composition comprising aceclidine and a pH of about 4.5 to about5.5, and having been stored at room temperature, such as for up to 6 months prior to administration of the composition to the eye of the individual.

[0449] In some as aspects, provided herein is a composition for use in treating presbyopia in an individual, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, and having been stored at room temperature for up to 6 months prior to administering to an eye of the individual.

[0450] In some embodiments, a composition for use in treating presbyopia in an individual comprises aceclidine and having a pH of about 4.5 to about 6 and having been stored at room temperature for up to 6 months prior to administering to an eye of the individual.

[0451] In some embodiments, a composition for use in treating presbyopia in an individual comprises aceclidine and having a pH of about 5 to about 6 and having been stored at room temperature for up to 6 months prior to administering to an eye of the individual.

[0452] In some aspects, provided herein is a method of treating presbyopia in an individual, the method comprising administering to the individual an ophthalmologically acceptable composition to an eye of the individual, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, and having been stored up to 12-18 months before being stored at room temperature, and having been stored at room temperature for up to 6 months prior to administration.

[0453] In some aspects, provided herein is a composition for use in treating presbyopia in an individual, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, and having been stored up to 12-18 months before being stored at room temperature, and having been stored at room temperature for up to 6 months prior to administering to an eye of the individual.

[0454] In some aspects, provided herein is a method of storing a composition described herein. In some embodiments, a composition described herein is stored in a container as described elsewhere herein.

[0455] In some embodiments, the method of storing comprises storing the composition for up to 18 months, up to 17 months, up to 16 months, up to 15 months, up to 14 months, up to 13 months, up to 12 months, up to 11 months, up to 10 months, up to 9 months, up to 8 months, up to 7 months, up to 6 months, up to 5 months, up to 4 months, up to 3 months, up to 2 months, or up to 1 month. In some embodiments, the method of storing comprises storing the composition for up to 12 months. In some embodiments, the method of storing comprises storing the composition for up to 9 months. In some embodiments, the method of storing comprises storing the composition for up to 6 months. In some embodiments, the method of storing comprises storing the composition for up to 4 months. In some embodiments, the method of storing comprises storing the composition for up to 2 months. In some embodiments, the method of storing comprises storing the composition for up to 1 month.

[0456] In some embodiments, the method of storing comprises storing the composition at a refrigerated temperature (e.g., 0 °C to 10°C or 2 °C to 8°C) for up to 18 months, up to 17 months, up to 16 months, up to 15 months, up to 14 months, up to 13 months, up to 12 months, up to 11 months, up to 10 months, up to 9 months, up to 8 months, up to 7 months, up to 6 months, up to 5 months, up to 4 months, up to 3 months, up to 2 months, or up to 1 month. In some embodiments, the method of storing comprises storing the composition at a refrigerated temperature for up to 12 months. In some embodiments, the method of storing comprises storing the composition at a refrigerated temperature for up to 9 months. In some embodiments, the method of storing comprises storing the composition at a refrigerated temperature for up to 6 months In some embodiments, the method of storing comprises storing the composition at a refrigerated temperature for up to 4 months. In some embodiments, the method of storing comprises storing the composition at a refrigerated temperature for up to 2 months. In some embodiments, the method of storing comprises storing the composition at a refrigerated temperature for up to 1 month.

[0457] In some embodiments, the method of storing comprises storing the composition at a room temperature (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for up to 18 months, up to 17 months, up to 16 months, up to 15 months, up to 14 months, up to 13 months, up to 12 months, up to 11 months, up to 10 months, up to 9 months, up to 8 months, up to 7 months, up to 6 months, up to 5 months, up to 4 months, up to 3 months, up to 2 months, or up to 1 month. In some embodiments, the method of storing comprises storing the composition at room temperature for up to 12 months. In some embodiments, the method of storing comprises storing the composition at room temperature for up to 9 months. In some embodiments, the method of storing comprises storing the composition at room temperature for up to 6 months. In some embodiments, the method of storing comprises storing the composition at room temperature for up to 4 months. In some embodiments, the method of storing comprises storing the composition at room temperature for up to 2 months. In some embodiments, the method of storing comprises storing the composition at room temperature for up to 1 month.

[0458] In some embodiments, the method of storing (e.g., overall, or cold storing) comprises storing the composition for at least 18 months (e.g., at least 17 months, at least 16 months, at least 15 months, at least 14 months, at least 13 months, at least 12 months, at least 11 months, at least 10 months, at least 9 months, at least 8 months, at least 7 months, at least 6 months, at least 5 months, at least 4 months, at least 3 months, at least 2 months, or at least 1 month). In some embodiments, the method of storing comprises storing the composition for at least 12 months. In some embodiments, the method of storing comprises storing the composition for at least 9 months. In some embodiments, the method of storing comprises storing the composition for at least 6months. In some embodiments, the method of storing comprises storing the composition for at least 4 months. In some embodiments, the method of storing comprises storing the composition for at least 2 months. In some embodiments, the method of storing comprises storing the composition for at least 1 month. In some embodiments, the method of storing comprises storing the composition for at least 7 weeks. In some embodiments, the method of storing comprises storing the composition for at least 6 weeks.

[0459] In some aspects, provided herein is a method of storing an ophthalmologically acceptable composition, the method comprising storing the composition for up to 6 months at room temperature, the composition comprising aceclidine and a pH of about 4 5 to about 5.5, wherein following the up to 6 months of storage at room temperature, the composition comprises at least 90% of the amount of aceclidine as prior to the up to 6 months of storage at room temperature.

[0460] In some aspects, provided herein is a method of storing an ophthalmologically acceptable composition, the method comprising storing the composition for up to 12-18 months followed by storing the composition for up to 6 months at room temperature, the composition comprising aceclidine and a pH of about 4.5 to about 5.5, wherein following the up to 6 months of storage at room temperature, the composition comprises at least 90% of the amount of aceclidine as prior to the up to 6 months of storage at room temperature.

[0461] In some embodiments, the method comprises storing the composition at refrigerated temperature for up to 12 to 18 months. For example, such a method could comprise storing the composition at refrigerated temperature for 0 to 12 months, for 0 to 18 months, for 12 months, for 18 months, at least 12 months, or at least 18 months. In some embodiments, the method comprises storing the composition at refrigerated temperature for up to 18 months. In some embodiments, the method comprises storing the composition at refrigerated temperature for up to 15 months. In some embodiments, the method comprises storing the composition at refrigerated temperature for up to 12 months. In some embodiments, the method comprises storing the composition at refrigerated temperature for up to 9 months. In some embodiments, the method comprises storing the composition at refrigerated temperature for up to 6 months. In some embodiments, the method comprises storing the composition at refrigerated temperature for up to 3 months.

[0462] In some embodiments, refrigerated temperature is about 0 degrees Celsius (°C) to about 10°C. In some embodiments, refrigerated temperature is about 2 degrees Celsius (°C) to about 8°C.

[0463] In some embodiments, the method of storing comprises or allows for storing the composition at an elevated temperature for a period of time. In some embodiments, an elevated temperature is from about 30°C to about 40°C. In some embodiments, an elevated temperature isfrom about 35°C to about 40°C. For example, during shipping, a vehicle or container that the composition is situated in can reach temperatures up to 40°C for a period of time. In another example, while situated in a warehouse or a stockroom, the composition can reach temperatures up to 40°C for a period of time. In some embodiments, the method of storing comprises storing the composition at a temperature up to about 40°C (e.g., about 30°C to about 40°C) for up to 1 month. In some embodiments, the method of storing comprises storing the composition at a temperature up to about 40°C (e.g., about 30°C to about 40°C) for up to 4 weeks. In some embodiments, the method of storing comprises storing the composition at a temperature up to about 40°C (e g., about 30°C to about 40°C) for up to 3 weeks. In some embodiments, the method of storing comprises storing the composition at a temperature up to about 40°C (e.g., about 30°C to about 40°C) for up to 2 weeks. In some embodiments, the method of storing comprises storing the composition at a temperature up to about 40°C (e.g., about 30°C to about 40°C) for up to 1 week. In some embodiments, the method of storing comprises storing the composition at a temperature up to about 40°C (e.g., about 30°C to about 40°C) for up to 10 days. In some embodiments, the method of storing comprises storing the composition at a temperature up to about 40°C (e.g., about 30°C to about 40°C) for up to 8 days. In some embodiments, the method of storing comprises storing the composition at a temperature up to about 40°C (e.g., about 30°C to about 40°C) for up to 7 days. In some embodiments, the method of storing comprises storing the composition at a temperature up to about 40°C (e.g., about 30°C to about 40°C) for up to 5 days.

[0464] In some embodiments, the method of storing comprises storing the composition at a refrigerated temperature (e.g., about 0°C to about 10°C) for a period of time, followed by storing the composition at a room temperature (e.g., about 15°C to about 30°C) for a period time.

[0465] In some embodiments, the method of storing comprises storing the composition at a refrigerated temperature (e.g., about 0°C to about 10°C) for a period of time, followed by storing the composition at an elevated temperature (e.g., about 30°C to about 40°C) for a period of time, followed by storing the composition at a room temperature (e.g., about 15°C to about 30°C) for a period time.

[0466] In some embodiments, the method of storing comprises storing the composition at an elevated temperature (e g., about 30°C to about 40°C) for a period of time, followed by storing the composition at a refrigerated temperature (e.g., about 0°C to about 10°C) for a period of time, followed by storing the composition at a room temperature (e g., about 15°C to about 30°C) for a period time.

[0467] In some embodiments, the composition may reach a room temperature while transitioning from a refrigerated temperature to an elevated temperature. In some embodiments,the composition may reach a room temperature while transitioning from an elevated temperature to a refrigerated temperature.

[0468] In some embodiments, the method of treating presbyopia in an individual comprises administering to the individual an ophthalmologically acceptable composition to an eye of the individual, the composition comprising aceclidine and having a pH of about 4.5 to about 6 and having been stored up to 12-18 months before being stored at room temperature, and having been stored at room temperature for up to 6 months prior to administration.

[0469] In some embodiments, a composition provided herein comprises a buffer, such as any suitable buffer (e g., a buffer suitable for ophthalmic use). In certain embodiments, the buffer is a citrate, such as sodium citrate, or an acetate, such as sodium acetate. In another embodiment, the buffer is a phosphate, Tris, or borate buffer. In certain embodiments, a composition provided herein comprises a buffer, such as in any suitable concentration (e.g., a concentration suitable for providing a pH provided herein).

[0470] In some embodiments, a composition provided herein comprises a buffer. In some embodiments, a composition described herein comprises any suitable buffer, such as a buffer that provides stability of a pH or pH range of the composition. In some embodiments, a buffer resists changes in the pH or pH range of the composition through neutralizing small amounts of changes in acidic or basic components of the composition.

[0471] In some embodiments, a buffer described herein is an acetate buffer, citrate buffer, phosphate buffer, borate buffers, or a combination thereof. In some embodiments, a buffer described herein is a citrate buffer.

[0472] In some embodiments, a composition described herein has a pH of less than about 7. In some embodiments, a composition described herein has a pH of 6 or less. In some embodiments, a composition described herein has a pH of about 4 to about 6. In some embodiments, a composition described herein has a pH of about 4.5 to about 6. In some embodiments, a composition described herein has a pH of about 5 to about 6. In some embodiments, a composition described herein has a pH of about 5.5 to about 6. In some embodiments, a composition described herein has a pH of about 4.5 to about 5.5. In some embodiments, a composition described herein has a pH of about 5 to about 5.5. In some embodiments, a composition described herein has a pH of about 5. In some embodiments, a composition described herein has a pH of about 5.5.

[0473] In some embodiments, a composition described herein has a pH of about 5 to about 6 and a (e.g., sodium citrate) buffer concentration of about 0.06 wt. % to about 0.1 wt. %. In some embodiments, a composition described herein has a pH of about 5 and a sodium citrate buffer concentration of about 0.06 wt. %. In some embodiments, a composition described herein has a pH of about 5 and a sodium citrate buffer concentration of about 0.08 wt. %. In someembodiments, a composition described herein has a pH of about 5 and a sodium citrate buffer concentration of about 0.1 wt. %. In some embodiments, a composition described herein has a pH of about 5.5 and a sodium citrate buffer concentration of about 0.06 wt %. In some embodiments, a composition described herein has a pH of about 5.5 and a sodium citrate buffer concentration of about 0.08 wt. %. In some embodiments, a composition described herein has a pH of about 5.5 and a sodium citrate buffer concentration of about 0.1 wt. %. In some embodiments, a composition described herein has a pH of about 6 and a sodium citrate buffer concentration of about 0.06 wt. %. In some embodiments, a composition described herein has a pH of about 6 and a sodium citrate buffer concentration of about 0 08 wt. %. In some embodiments, a composition described herein has a pH of about 6 and a sodium citrate buffer concentration of about 0.1 wt. %.

[0474] In some embodiments, an ophthalmological drug in a composition as described herein is stable irrespective to the concentration of the buffer in the composition. In some embodiments, a composition comprising an ophthalmological drug and a first concentration of a buffer has a stability of at least about 90% after storing, and an otherwise similar composition comprising a second (different) concentration of the buffer also has a stability of at least about 90% after identical storing conditions. For example, a composition comprising aceclidine and a buffer at a concentration of 0.1 wt. %, 0.08 wt. %, or 0.06 wt. % has a stability (of aceclidine) of at least 90% at a temperature of about 25 degrees Celsius or 5 degrees Celsius for up to 6 to 18 months. Specifically, for example, a composition comprising aceclidine and a buffer at a concentration of 0.1 wt. % has a stability of aceclidine of at least 90% after storing at room temperature (e.g., 25 degrees Celsius) for 6 months, and an otherwise similar composition comprising a buffer at a concentration of 0.08 wt. % has a stability of about 90% or more after storing at room temperature for 6 months, and likewise, an otherwise similar composition comprising a buffer at a concentration of 0.06 wt. % has a stability of at least 90% after storing at room temperature for 6 months. Specifically, for example, a composition comprising aceclidine and a buffer at a concentration of 0.1 wt. % has a stability of aceclidine of at least 90% after storing at a refrigerated temperature (e.g., 5 degrees Celsius) for 18 months, and an otherwise similar composition comprising a buffer at a concentration of 0.08 wt. % has a stability of at least 90% after storing at refrigerated temperature for 18 months, and likewise, an otherwise similar composition comprising a buffer at a concentration of 0.06 wt. % has a stability of at least 90% after storing at refrigerated temperature for 18 months.

[0475] In some embodiments, a greater chemical stability of the ophthalmological drug extends the length of time a composition can be stored at a room temperature. In some embodiments, greater chemical stability of the ophthalmological drug in a composition as described hereinextends the length of time a composition can be stored at a room temperature 2-fold, or greater, relative to the ophthalmological drug alone in aqueous solution.

[0476] In some embodiments, the ophthalmological drug in any (aqueous) composition described herein is chemically stable for a longer period of time relative to the ophthalmological drug in water or a saline solution alone. In some embodiments, the ophthalmological drug in any (aqueous) composition described herein is chemically stable after storing at room temperature for a longer period of time relative to the ophthalmological drug in water or a saline solution alone.

[0477] In certain embodiments, a composition provided herein comprises citrate or a salt thereof(e g., sodium citrate) or acetate or a salt thereof (e g., sodium acetate) in a concentration of about 0.01% to about 1% (e g., about 0.05% to about 0.2%). In specific embodiments, a composition provided herein comprises citrate or a salt thereof (e.g., sodium citrate) in a concentration of about 0.01% to about 0.1% (e.g., about 0.05% to about 0.08%). In some embodiments, a buffering agent is present or formulated into the ophthalmological composition provided herein is in a concentration of about 0.05 wt. % to about 0.15 wt. % (e.g., formulated with sodium citrate at a concentration of about 0.08 wt. %).

[0478] In some embodiments, a composition described herein comprises an ophthalmological drug. In some embodiments, the ophthalmological drug is aceclidine or a salt thereof.

[0479] In some embodiments, a composition described herein comprises an ophthalmological drug and a viscosity agent.

[0480] In some embodiments, a composition described herein comprises an ophthalmological drug and a surfactant.

[0481] In some embodiments, a composition described herein comprises an ophthalmological drug and a buffer.

[0482] In some embodiments, a composition described herein comprises an ophthalmological drug and tonicity agent.

[0483] In some embodiments, a composition described herein comprises an ophthalmological drug and an antioxidant.

[0484] In some embodiments, a composition described herein comprises an ophthalmological drug, a viscosity agent, and a surfactant.

[0485] In some embodiments, a composition described herein comprises an ophthalmological drug, a viscosity agent, a surfactant, and a buffer.

[0486] In some embodiments, a composition described herein comprises an ophthalmological drug, a viscosity agent, a surfactant, and a tonicity agent.

[0487] In some embodiments, a composition described herein comprises an ophthalmological drug, a viscosity agent, a surfactant, a buffer, and a tonicity agent.

[0488] In some embodiments, a composition described herein comprises an ophthalmological drug, a viscosity agent, a surfactant, a buffer, a tonicity agent, and an antioxidant.

[0489] In some embodiments, a composition provided herein comprises a viscosity agent, such as any suitable viscosity agent. In some instances, a viscosity agent (e.g., a viscosity enhancing agent or thickening agent, which terms are used interchangeably herein) enhances permeation of an ophthalmological drug described herein into an eye (e.g., cornea) of an individual described herein. In some instances, a viscosity agent (e.g., a viscosity enhancing agent) increases the residence time of an ophthalmological drug described herein on an eye of an individual described herein. In some embodiments, an ophthalmological composition provided herein is an eye drop (e.g., slightly viscous ophthalmic solution).

[0490] In some embodiments, the composition further comprises a viscosity agent described herein (e g., a cellulose derivative), such as at a concentration described herein. In some embodiments, a viscosity agent provided herein is guar gum, hydroxypropyl-guar (“hp-guar”), xanthan gum, alginate, chitosan, gelrite, hyaluronic acid, dextran, Carbopol® (polyacrylic acid or carbomer) including Carbopol® 900 series including Carbopol® 940 (carbomer 940), Carbopol® 910 (carbomer 910) and Carbopol® 934 (carbomer 934), cellulose derivatives such as high molecular weight carboxymethyl cellulose (“CMC”), methylcellulose, methyl cellulose 4000, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, hydroxyl propyl methyl cellulose 2906, carboxypropylmethyl cellulose, hydroxypropylethyl cellulose, and hydroxyethyl cellulose, polyethylene glycol, polyvinyl alcohol, polyvinyl chloride, polyvinyl pyrrolidone, gellan, carrageenan, alginic acid, carboxyvinyl polymer or combinations thereof. In certain embodiments, a viscosity agent is a cellulose derivative. In some embodiments, a viscosity agent is a high molecular weight carboxymethyl cellulose (CMC). In some embodiments, a viscosity agent is methylcellulose, methyl cellulose 4000, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, hydroxyl propyl methyl cellulose 2906, carboxypropylmethyl cellulose, hydroxy propyl ethyl cellulose, hydroxyethyl cellulose, or a combination thereof. In some embodiments, a viscosity agent is hydroxypropylmethyl cellulose (HPMC).

[0491] In some embodiments, a composition described herein comprises any suitable concentration of a viscosity agent. In some embodiments, a concentration of a viscosity agent for a composition described herein is 5 wt. %, or less. In some embodiments, the concentration of a viscosity agent in a composition described herein is about 0.01 wt. % to about 5 wt. %. In some embodiments, the concentration of a viscosity agent in a composition described herein is about 0.1 wt. % to about 4 wt. %. In some embodiments, the concentration of a viscosity agent in a composition described herein is about 0.5 wt. % to about 2 wt. %. In some embodiments, theconcentration of a viscosity agent in a composition described herein is about 1 wt. % to about 2 wt. %. In some embodiments, the concentration of a viscosity agent in a composition described herein is about 1 wt % to about 1.5 wt. % (e.g., about 1.25 wt. %).

[0492] In some embodiments, the composition provided herein, such as enclosed in a chamber of a container (e.g., of a system) provided herein, has any suitable viscosity. In certain embodiments, the composition has relatively high viscosity at low shear (e.g., 0 or 1 per second shear rate), such as to inhibit degradation of active ingredients and / or to improve residence time on an ocular surface upon installation.

[0493] In some embodiments, the composition provided herein, such as enclosed in a chamber of a container (e g., of a system) provided herein, has a viscosity at low shear (e.g., 0 or 1 per second shear rate) of at least 50 centipoise (cP). In specific embodiments, the viscosity of low shear is about 100 to about 1,000 cP. In more specific embodiments, the viscosity of low shear is about 200 to about 500 cP. In still more specific embodiments, the viscosity of low shear is about 300 to about 400 cP. In certain embodiments, any viscosity agent, such as a viscosity agent provided herein, is included in the composition in an amount sufficient to provide a desired viscosity (e g., at low shear), such as a viscosity described herein.

[0494] In some embodiments, the composition provided herein, such as enclosed in a chamber of a container (e.g., of a system) provided herein, has a viscosity of at least 500 centipoise (cP). In specific embodiments, the viscosity is about 500 to about 1,500 cP. In more specific embodiments, the viscosity is about 600 to about 1,200 cP. In still more specific embodiments, the viscosity is about 750 to about 1000 cP. In some embodiments, the viscosity is about 800 to about 900 cP. In some instances, the viscosity is measured at 20 degrees Celsius ± 0.1 degrees Celsius, such as at 12 rpm. In some instances, protocols for determining viscosity are set forth in the examples provided herein.

[0495] In some embodiments, the composition further comprises a surfactant (e.g., a nonionic surfactant), such as at a concentration described herein. In some embodiments, the surfactant is alpha cyclodextrin, beta cyclodextrin, or gamma cyclodextrin. In some embodiments, a beta cyclodextrin is 2-hydroxypropyl beta-cyclodextrin (“HPpCD”). In some embodiments, a beta cyclodextrin is sulfobutyl ether derivative of P-cyclodextrin (Captisol®). In some embodiments, a surfactant is a polyoxyl alkyl. In some embodiments, a surfactant is polyoxyl 40 stearate or polyoxyl 35 castor oil. In some embodiments, a surfactant is a poloxamer. In some embodiments, a surfactant is poloxamer 108 or poloxamer 407. In some embodiments, a surfactant is a polysorbate. In some embodiments, a surfactant is polysorbate 80 or Brij® 35 (Brij is a registered trademark of Uniqema Americas LLC). In some embodiments, a surfactant is polysorbate 80. In some embodiments, a surfactant is a polysorbate, a tyloxapol, a poloxamer, a cyclodextrin, vitaminE TPGS, a polyoxyl castor oil, a polyoxyl stearate, polyethylene glycol, a polyoxyethylene glycol alkyl ether or 2-[[10,13-dimethyl-17-(6-methylheptan-2-yl)-2,3,4,7,8,9,l l,12,14,15,16,17- dodecahydro-lH-cyclopenta[a]phenanthren-3-yl]oxy]ethanol. In some embodiments, a surfactant is Pol oxamer 80, Pol oxamer 188, Poloxamer 407, Polysorbate 20, Polysorbate 80, ionically charged (e.g., anionic) beta - cyclodextrins with or without a butyrated salt (Captisol®) 2- hydroxypropyl beta cyclodextrin (“HPpCD”), alpha cyclodextrins, gamma cyclodextrins, Polyoxyl 35 castor oil, and Polyoxyl 40 hydrogenated castor oil or combinations thereof.

[0496] In some embodiments, a composition described herein comprises any suitable concentration of a surfactant. In some embodiments, a composition described herein comprises a concentration of surfactant at or above the critical micellar concentration for the composition. In some embodiments, the concentration of a surfactant in a composition described herein is about 15 wt. %, or less. In some embodiments, the concentration of a surfactant in a composition described herein is about 0.1 wt. % to about 15 wt. %. In some embodiments, the concentration of a surfactant in a composition described herein is about 1 wt. % to about 10 wt. %. In some embodiments, the concentration of a surfactant in a composition described herein is about 1 wt. % to about 10 wt. %. In some embodiments, the concentration of a surfactant in a composition described herein is about 1 wt. % to about 5 wt. %. In some embodiments, the concentration of a surfactant in a composition described herein is about 2 wt. % to about 5 wt. %. In some embodiments, the concentration of a surfactant in a composition described herein is about 3 wt. % to about 5 wt. %.

[0497] In some instances, a surfactant provided herein (e.g., polysorbate 80) functions in a composition described herein as a lubricant when administered to an ocular surface such as to relieve burning, irritation, or discomfort in the eye / ocular surface.

[0498] In some embodiments, an ophthalmological composition provided herein comprises a lubricant. In some embodiments, the lubricant is a surfactant provided herein. In some embodiments, the concentration of a lubricant provided herein is about 3 wt. % to about 5 wt. % (e.g., about 4 wt. %).

[0499] In some embodiments, an ophthalmological composition provided herein comprises a chelating agent. In some embodiments, the chelating agent is edetate (e.g., formulated with edetate disodium dihydrate). In some embodiments, the concentration of a chelating agent provided herein is about 0.05 wt. % to about 0.2 wt. % (e.g., formulated with edetate disodium dihydrate at a concentration of about 0.1 wt. %).

[0500] In some embodiments, a composition described herein comprises atonicity agent. In some embodiments, composition described herein comprises any suitable tonicity agent. In some instances, a tonicity agent reduces irritation on the eye of an individual when applied to the eye.In some instances, a tonicity agent reduces irritation at the application site (e.g., eye) by preventing or reducing osmotic shock. Osmotic shock may occur at the application site upon a sudden change in salt concentration. In some embodiments, a composition described herein comprising atonicity agent reduces or prevents osmotic shock at the application site relative to an otherwise similar composition not comprising a tonicity agent.

[0501] In some embodiments, a composition provided herein comprises any suitable tonicity agent. In specific embodiments, the tonicity agent is a polyol or salt. In some embodiments, a tonicity agent is a polyol. In some embodiments, a polyol is two or more hydroxyl groups. In some embodiments, a polyol is glycerin, pentaerythritol, ethylene glycol, sucrose, dextrose, glucose, mannitol, glycerol, erythritol, lactitol, xylitol, sorbitol, isosorbide, propylene glycol, maltitol, threitol, arabitol, ribitol, or a combination thereof. In some embodiments, a tonicity agent is mannitol. In some embodiments, a tonicity agent is glycerin. In some embodiments, a tonicity agent is dextrose. In some embodiments, a tonicity agent is a salt. In some embodiments, a tonicity agent is sodium chloride or potassium chloride. In some embodiments, a composition described herein comprises any suitable concentration of a tonicity agent. In some embodiments, the concentration of a tonicity agent in a composition described herein is about 5 wt. %, or less. In some embodiments, the concentration of a tonicity agent in a composition described herein is about 4 wt. %, or less. In some embodiments, the concentration of a tonicity agent in a composition described herein is about 0.01 wt. % to about 4 wt. %. In some embodiments, the concentration of a tonicity agent in a composition described herein is about 1 wt. % to about 4 wt. %. In some embodiments, the concentration of a tonicity agent in a composition described herein is about 2 wt. % to about 3 wt. % (e.g., about 2.5 wt. %).

[0502] In some embodiments, a composition provided herein is preservative-free. In some embodiments, a composition provided herein is substantially preservative-free. In some embodiments, the composition has no detectable amount of a preservative or contains less than about 0.5%, less than about 0.4%, less than about 0.3%, less than about 0.2%, less than about 0.1%, less than about 0.01%, or less than about 0.001% of a preservative. In certain embodiments, a composition provided herein is free of benzalkonium chloride (BAK). In some embodiments, a composition is free of or substantially free of benzalkonium chloride (BAK), sorbic acid, sodium ascorbate, sodium bisulfate, sodium metabisulfite, n-acetyl cysteine, thimerosal, phenylmercuric acetate, phenylmercuric nitrate, perborate, benzyl alcohol, cetrimonium, sodium perborate, oxy chloro complex, SofZia, polyquatemium-1, chlorobutanol, polyhexamethylene biguanide, or a combination thereof.

[0503] In some embodiments, an ophthalmological drug in a composition as described herein is stable irrespective to the presence of a preservative in the composition. In some embodiments, acomposition comprising an ophthalmological drug and a preservative has a stability of at least about 90% after storing, and an otherwise similar composition comprising no preservative also has a stability of at least about 90% after identical storing conditions For example, a composition comprising aceclidine and benzalkonium chloride (BAK) has a stability of about 98% after 18 months when stored at 5 degrees Celsius, and an otherwise similar composition comprising no BAK also has a stability of about 98% after identical storing conditions (e.g., after 18 months when stored at 5 degrees Celsius).

[0504] Compositions comprising aceclidine with a preservative (e.g., BAK) and without BAK can have identical or substantially identical stability of aceclidine under similar storing conditions as shown herein. For example, when stored at 25 degrees Celsius, the compositions comprise 96% of the initial concentration of aceclidine after 6 months, with or without the presence of the preservative (e.g., BAK). For example, when stored at 5 degrees Celsius, the compositions comprise 98% of the initial concentration of aceclidine after 18 months, with or without the presence of the preservative (e.g., BAK).

[0505] In some embodiments, a composition described herein comprises any suitable medium for the ophthalmological drug. In some embodiments, a medium of a composition described herein is suitable for application to the eye of an individual. In some embodiments, the medium of the composition comprises water. In some embodiments, the composition is aqueous. In some aspects, the compositions described herein are disposed in any suitable container (e.g., drop bottle).

[0506] In certain embodiments, the system and / or container and / or composition is sterile. In some instances, use of a single use container, such as described herein, allows for the use of a preservative free composition. In addition, efficacious use of a two-drop dose, wherein the two drops are administered in a short time period (e.g., only 2 minutes versus 5 minutes or more) allows for the use of a single container that may be used to administer two drops to a single eye or four drops to two eyes of an individual. In some instances, if longer periods of time were used, there could be a higher chance of spillage, which may be result in insufficient volume of composition for appropriate dosing. It is surprising that use of two drop administration within 2 minutes facilitates long term efficacious use (e.g., relative to a single drop dose) and it is surprising that such a short time period may facilitate extended duration of efficacy (e.g., and that a longer delay, such as 5 minutes or more is not required).

[0507] In some embodiments, a composition provided herein is clear and colorless. In some instances, it is surprising that a composition comprising the components such as described herein is clear and colorless. In certain embodiments, such characteristics are important for commercial use so that end users can readily determine whether or not a composition may be suitable for use. free.

[0508] In some embodiments, provided herein is a method for treating presbyopia in an individual (e.g., in need thereof). In specific embodiments, the method comprises administering to the individual (e g., at least one eye thereof) a (e g , ophthalmological) composition, such as any composition described herein. In some embodiments, the method comprises providing a system or container provided herein, such as wherein the method comprises opening a container (e.g., of a system) provided herein.

[0509] In certain embodiments, a method provided herein comprises opening a (e.g., single use) container, such as a container provided herein. In specific embodiments, opening the container provides an opening in the container In some embodiments, the opening provides access to an enclosure of the container. In some embodiments, a composition provided herein is configured within the container.

[0510] In some embodiments, a method provided herein comprises administering to an (e.g., eye of the) individual a drop of a composition from the container (e.g., wherein the composition is configured within the container, such as an enclosure thereof), such as through an opening of the container. In certain embodiments, the method further comprises administering to (e.g., an eye of the) individual a second drop of a composition from the container. In specific embodiments, the second drop is administered 30 seconds to 4 minutes (or less than 5 minutes) after the first drop. In more specific embodiments, the second drop is administered 1 to 3 minutes after the first drop. In still more specific embodiments, the second drop is administered about 2 minutes after the first drop.

[0511] In certain embodiments, a method provided herein comprising administering a first and a second drop of a composition (e.g., from a container provided herein) to a first eye of an individual and a third and a fourth drop of a composition (e.g., from a container provided herein) to a second eye of an individual. In specific embodiments, the first, second, third, and fourth drops are all administered from a single container. In some embodiments, the second drop is administered 30 seconds to 4 minutes (or less than 5 minutes) after the first drop. In more specific embodiments, the second drop is administered 1 to 3 minutes after the first drop. In still more specific embodiments, the second drop is administered about 2 minutes after the first drop. In certain embodiments, the fourth drop is administered 30 seconds to 4 minutes (or less than 5 minutes) after the third drop. In more specific embodiments, the fourth drop is administered 1 to 3 minutes after the third drop. In still more specific embodiments, the fourth drop is administered about 2 minutes after the third drop. In certain embodiments, the first and third drop are administered prior to the second and third drop. In some embodiments, the third drop is administered immediately (within 30 seconds) following administration of the first drop. In certain embodiments, the fourthdrop is administered immediately (within 30 seconds) following administration of the second drop.

[0512] In some embodiments, additional compositions are similarly administered on subsequent days. For example, in certain embodiments, a second system is provided, with a second container being opened and drops administered to the eye(s) of the individual on a second day. In some embodiments, a third system is provided, with a third container being opened and drops administered to the eye(s) of the individual on a third day. In certain embodiments, additional containers are similarly used. In some embodiments, a kit is provided comprising two or more such containers and packaging material enclosing the two or more containers prior to use. In certain embodiments, three or more containers are provided in the kit. In specific embodiments, five containers are provided in the kit (e.g., to facilitate business weekday use of the compositions / sy stems).

[0513] In some embodiments, provided herein is a kit comprising at least two similar or identical systems and a package (e.g., housing the two or more systems). In some embodiments, the two similar or identical systems each comprise a separate container and separate but identical compositions, such as wherein the composition is (or the compositions are) any composition described herein. In certain instances, the containers of the kit are optionally connected to one another prior to use, such as in a manner whereby a container can be detached from the other containers prior to use (e.g., prior to opening and dispensing a composition therefrom).

[0514] In certain embodiments, a method provided comprises storing a composition (e g., the system and container enclosing the composition) at room temperature (e.g., up to 25 °C) prior to use. In some embodiments, a method provided herein comprising using a composition (e.g., the system and container enclosing the composition) that has been stored at room temperature (e g., up to 25 °C) prior to use. In certain embodiments, the composition is or has been stored for (e.g., up to) 3 months at room temperature (e.g., up to 25 °C). In certain embodiments, the composition is or has been stored for (e.g., up to) 6 months at room temperature (e.g., up to 25 °C). In some instances, a composition comprises at least 90% of the initial amount (e.g., upon initial formulation or immediately prior to room temperature storage) of aceclidine in the composition upon administration (e.g., after storing at room temperature as described herein). In specific instances, room temperature is a temperature above refrigerated temperature (e.g., about 8 °C). In some embodiments, room temperature is up to 25 °C. In some embodiments, room temperature is about 8 °C to about 25 °C, about 10 °C to about 25 °C, about 15 °C to about 25 °C, about 20 °C to about 25 °C, about 23 °C to about 25 °C, about 8 °C to about 27 °C, about 10 °C to about 27 °C, about 15 °C to about 27 °C, about 20 °C to about 27 °C, or about 23 °C to about 27 °C. In some embodiments, room temperature is ambient temperature. In some embodiments, roomtemperature is from about 15 °C to about 30°C. In some embodiments, room temperature is from about 15°C to about 25°C. In some embodiments, room temperature is from about 15°C to about 20°C. In some embodiments, room temperature is from about 20°C to about 30°C. In some embodiments, room temperature is from about 25°C to about 30°C. In some embodiments, room temperature is about 25°C.

[0515] In certain embodiments, a method provided comprises storing a composition (e g., the system and container enclosing the composition) at refrigerated temperature (e.g., about 2 °C to about 8 °C) prior to use. In some embodiments, a method provided herein comprising using a composition (e g., the system and container enclosing the composition) that has been stored at refrigerated temperature (e.g., about 2 °C to about 8 °C) prior to use. In certain embodiments, the composition is or has been stored for (e g., up to) 12 months at refrigerated temperature (e g., about 2 °C to about 8 °C) prior to use. In certain embodiments, the composition is or has been stored for (e.g., up to) 18 months at refrigerated temperature (e.g., about 2 °C to about 8 °C) prior to use. In some instances, a composition comprises at least 95% of the initial amount (e.g., upon initial formulation or immediately prior to room temperature storage) of aceclidine in the composition upon administration or after storing at refrigerated temperature, such as described herein. In some instances, a composition comprises at least 90% of the initial amount (e.g., upon initial formulation) of aceclidine in the composition upon administration (e.g., after storing at refrigerated and room temperature, such as described herein).

[0516] In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for up to 6 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for up to 8 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for up to 10 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for up to 11 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for up to 12 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for up to 14 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for up to 16 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for up to 18 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for up to 20 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for upto 22 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for up to 24 months. After storage, the composition can retain at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the initial amount of aceclidine (e.g., that was present upon initial formulation or immediately prior to storage).

[0517] In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for at least 6 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for at least 8 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for at least 10 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e g., at 2 °C to 8 °C), for at least 11 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for at least 12 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for at least 14 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e g., at 2 °C to 8 °C), for at least 16 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for at least 18 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for at least 20 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for at least 22 months. In some embodiments, a method provided herein comprises storing a composition at refrigerated temperature, (e.g., at 2 °C to 8 °C), for at least 24 months. After storage, the composition can retain at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the initial amount of aceclidine (e.g., that was present upon initial formulation or immediately prior to storage).

[0518] In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for up to 1 week. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for up to 2 weeks. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for up to 3 weeks. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20- 25°C, or 23-25°C) for up to 1 month. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for up to 2 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for up to 3 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for up to 4 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for up to 5 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for up to 6 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20- 25°C, or 23-25°C) for up to 8 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23- 25°C) for up to 10 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for up to 12 months. After storage, the composition can retain at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the initial amount of aceclidine (e g., that was present upon initial formulation or immediately prior to storage).

[0519] In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for at least 1 week. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for at least 2 weeks. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for at least 3 weeks. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20- 25°C, or 23-25°C) for at least 1 month. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23- 25°C) for at least 2 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for at least 3 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for at least 4 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for at least 5 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for at least 6 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C) for at least 8 months. In some embodiments, a method comprisesstoring a composition at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20- 25°C, or 23-25°C) for at least 10 months. In some embodiments, a method comprises storing a composition at room temperature as disclosed herein (e g., up to 25°C, 15-25°C, 20-25°C, or 23- 25°C) for at least 12 months. After storage, the composition can retain at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the initial amount of aceclidine (e g., that was present upon initial formulation or immediately prior to storage).

[0520] In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for up to 3 days. In some embodiments, a method comprises storing a composition at elevated temperature (e g , about 40 °C, about 37 °C, or about 37-40 °C) for up to 5 days. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for up to 6 days. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for up to 7 days. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for up to 8 days. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for up to 9 days. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for up to 10 days. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for up to 2 weeks. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for up 4 weeks. After storage, the composition can retain at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the initial amount of aceclidine (e g., that was present upon initial formulation or immediately prior to storage).

[0521] In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 3 days. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 5 days. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 6 days. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 7 days. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 8 days. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 9 days. In some embodiments, a method comprises storing a composition at elevatedtemperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 10 days. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 2 weeks. In some embodiments, a method comprises storing a composition at elevated temperature (e.g., about 40 °C, about 37 °C, or about 37-40 °C) for at least 3 weeks. After storage, the composition can retain at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the initial amount of aceclidine (e.g., that was present upon initial formulation or immediately prior to storage).

[0522] In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 1 month, and optionally (iii) elevated temperature for up to 8 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 2 months, and optionally (iii) elevated temperature for up to 8 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 4 months, and optionally (iii) elevated temperature for up to 8 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 6 months, and optionally (iii) elevated temperature for up to 8 days.

[0523] In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 1 month, and optionally (iii) elevated temperature for up to 4 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 2 months, and optionally (iii) elevated temperature for up to 4 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 4 months, and optionally (iii) elevated temperature for up to 4 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 6 months, and optionally (iii) elevated temperature for up to 4 days.

[0524] In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 1 month, and optionally (iii) elevated temperature for up to 8 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 2 months, and optionally (iii) elevated temperature for up to 8 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 4 months, and optionally (iii) elevated temperature for up to 8 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 6 months, and optionally (iii) elevated temperature for up to 8 days.

[0525] In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 1 month, and optionally (iii) elevated temperature for up to 4 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 2 months, and optionally (iii) elevated temperature for up to 4 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 4 months, and optionally (iii) elevated temperature for up to 4 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 6 months, and optionally (iii) elevated temperature for up to 4 days.

[0526] In some embodiments, a method comprises storing a composition: (i) at refrigerated temperature (e g., at 2 °C to 8 °C), (ii) at room temperature as disclosed herein (e.g., up to 25°C, 15-25°C, 20-25°C, or 23-25°C), and optionally (iii) at elevated temperature (e g., about 40 °C, about 37 °C, or about 37-40 °C). After the combined periods of storage at refrigerated temperature, room temperature, and optionally elevated temperature, the composition can retain at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the initial amount of aceclidine (e.g., that was present upon initial formulation or immediately prior to storage).

[0527] In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 1 month, and optionally (iii) elevated temperature for at least 8 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 2 months, and optionally (iii) elevated temperature for at least 8 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 4 months, and optionally (iii) elevated temperature for at least 8 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 6 months, and optionally (iii) elevated temperature for at least 8 days.

[0528] In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 1 month, and optionally (iii) elevated temperature for at least 4 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 2 months, and optionally (iii) elevated temperature for at least 4 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months, (ii) room temperature for at least 4 months, and optionally (iii) elevated temperature for at least 4 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 12 months,(ii) room temperature for at least 6 months, and optionally (iii) elevated temperature for at least 4 days.

[0529] In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 1 month, and optionally (iii) elevated temperature for at least 8 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 2 months, and optionally (iii) elevated temperature for at least 8 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 4 months, and optionally (iii) elevated temperature for at least 8 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 6 months, and optionally (iii) elevated temperature for at least 8 days.

[0530] In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 1 month, and optionally (iii) elevated temperature for at least 4 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 2 months, and optionally (iii) elevated temperature for at least 4 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 4 months, and optionally (iii) elevated temperature for at least 4 days. In some embodiments, the method comprises storage at (i) refrigerated temperature for at least 18 months, (ii) room temperature for at least 6 months, and optionally (iii) elevated temperature for at least 4 days.

[0531] In certain embodiments, a composition provided herein has been stored at a temperature from about 0 degrees Celsius to about 10 degrees Celsius, such as for up to 24 months. In certain embodiments, a composition provided herein has been stored at a temperature from about 2 degrees Celsius to about 8 degrees Celsius, such as for up to 24 months.

[0532] In certain embodiments, a composition provided herein has been stored at a temperature from about 0 degrees Celsius to about 10 degrees Celsius, such as for up to 12-18 months. In certain embodiments, a composition provided herein has been stored at a temperature from about 2 degrees Celsius to about 8 degrees Celsius, such as for up to 12-18 months.

[0533] In certain embodiments, a composition provided herein has been stored up to 24 months (e.g., at 2 degrees Celsius to 8 degrees Celsius). In certain embodiments, a composition provided herein comprises at least 90 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 24 months (e.g., at 2 degrees Celsius to 8 degrees Celsius). In certain embodiments, a composition provided herein comprisesat least 95 wt. % of the initial amount (e.g., the amount of aceclidine in the composition prior to such storage) of aceclidine after storage for 24 months (e.g., at 2 degrees Celsius to 8 degrees Celsius).

[0534] In certain embodiments, a composition provided herein has been stored u...

Claims

CLAIMSWhat is claimed is:

1. A system comprising a single-patient-use container and an ophthalmological composition, wherein:(a) the ophthalmological composition comprises aceclidine or a salt thereof; and(b) the single-patient-use container comprises an enclosed chamber, the ophthalmological composition being configured within the enclosed chamber, the single-patient-use container being configured to be irreversibly opened, and the single-patient-use container being configured, when open, to dispense a first drop and a second drop of the ophthalmological composition through an opening.

2. The system of claim 1, wherein the single-patient-use container is a vial or an ampoule.

3. The system of claim 1, wherein the single-patient-use container comprises a container body, a container neck, and a container head, the container body and the container head having a larger cross-sectional dimension than the container neck, the enclosed chamber extending from the container body into the container neck, and the single-patient-use container being configured to be opened at the container neck.

4. The system of any one of the preceding claims, wherein prior to opening, the enclosed chamber is sterile.

5. The system of any one of the preceding claims, wherein the container is transparent.

6. The system of any one of the preceding claims, wherein the container comprises polyethylene.

7. The system of any one of the preceding claims, wherein the container comprises a low- density polyethylene (LDPE).

8. The system of any one of the preceding claims, wherein the system comprises about 0.2 mL to about 2 mL of the ophthalmological composition.

9. The system of any one of the preceding claims, wherein the system comprises about 0.2 mL to about 1 mL of the ophthalmological composition.

10. The system of any one of the preceding claims, wherein the system comprises about 0.5 mL of the ophthalmological composition.

11. The system of any one of the preceding claims, wherein the ophthalmological composition comprises aceclidine or a salt thereof in a concentration of about 1 wt. % to about 2 wt. %.

12. The system of any one of the preceding claims, wherein the ophthalmological composition comprises aceclidine or a salt thereof in a concentration of about 1.75 wt. %.

13. The system of any one of the preceding claims, wherein the ophthalmological composition further comprises brimonidine.

14. The system of any one of the preceding claims, wherein the ophthalmological composition comprises brimonidine or a salt thereof in a concentration of about 0.05 wt. % to about 0.15 wt. %.

15. The system of any one of the preceding claims, wherein the composition comprises brimonidine or a salt thereof in a concentration of about 0.08 wt. %.

16. The system of any one of the preceding claims, wherein the ophthalmological composition comprises aceclidine hydrochloride in a concentration of about 1.75 wt. % and brimonidine tartrate in a concentration of about 0.08 wt. %.

17. The system of any one of the preceding claims, wherein the system comprises a package and at least 3 identical containers and at least 3 identical ophthalmological compositions.

18. A method for treating presbyopia in an individual (e.g., in need thereof), the method comprising: opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition, wherein the second drop is administered about 2 minutes after the first drop is administered to the first eye, wherein the ophthalmological composition comprises aceclidine or a salt thereof.

19. The method of claim 18, further comprising administering a third drop and a fourth drop of the ophthalmological composition to a second eye of the individual, wherein the fourth drop is administered to the second eye about 2 minutes after the third drop is administered to the second eye.

20. The method of claim 19, wherein the third drop is administered to the second eye prior to the second drop being administered to the first eye.

21. The method of any one of claims 18-20, further comprising opening a second single-patient-use container on a second day and subsequently administering to the first eye of the individual a second container first drop and a second container second drop of a second container ophthalmological composition, wherein the second container ophthalmological composition comprises aceclidine or a salt thereof, thesecond container second drop is administered to the first eye of the individual 2 minutes after the second container first drop is administered to the first eye, and the first and second days are different days.

22. The method of any one of claims 18-21, wherein the ophthalmological composition is an ophthalmological composition of any one of claims 1-17.

23. The method of any one of claims 21-22, wherein the second container ophthalmological composition is an ophthalmological composition of any one of claims 1-17.

24. The method of any one of claims 21-23, wherein the ophthalmological composition and the second container ophthalmological composition are identical.

25. A method for treating presbyopia and improving distance vision in an individual (e.g., in need thereof), the method comprising: opening a first single-patient-use container on a first day and subsequently administering to a first eye of the individual a first drop and a second drop of an ophthalmological composition, wherein the second drop is administered about 2 minutes after the first drop is administered to the first eye, wherein the ophthalmological composition comprises aceclidine or a salt thereof.

26. The method of claim 25, further comprising administering to a second eye of the individual a third drop and a fourth drop of the ophthalmological composition, wherein the fourth drop is administered to the second eye about 2 minutes after the third drop is administered to the second eye.

27. The method of claim 26, wherein the third drop is administered to the second eye prior to the second drop being administered to the first eye28. The method of any one of claims 25-27, further comprising opening a second single-patient-use container on a second day and subsequently administering to the first eye of the individual a second container first drop and a second container second drop of a second container ophthalmological composition, wherein the second container ophthalmological composition comprises aceclidine or a salt thereof, the second container second drop is administered to the first eye of the individual 2 minutes after the second container first drop is administered to the first eye, and the first and second days are different days.

29. The method of any one of claims 25-28, wherein the ophthalmological composition is the ophthalmological composition of any one of claims 1-17.

30. The method of any one of claims 28-29, wherein the second container ophthalmological composition is the ophthalmological composition of any one of claims 1-17.

31. The method of any one of claims 28-30, wherein the ophthalmological composition and the second container ophthalmological composition are identical.

32. The method of any one of the preceding claims, wherein the individual has moderate presbyopia.

33. The method of any one of the preceding claims, wherein the individual has advanced presbyopia.

34. The method of any one of the preceding claims, wherein the individual has an improvement in near vision (e.g., visual acuity at 40 cm) of at least 4 lines.

35. The method of any one of the preceding claims, wherein the individual has an improvement in near vision (e.g., visual acuity at 40 cm) of at least 5 lines.

36. The method of any one of the preceding claims, wherein the individual has an improvement in near vision (e.g., visual acuity at 40 cm) of at least 6 lines.

37. The method of any one of the preceding claims, wherein the individual has an improvement in distance vision (e g., visual acuity at 4 m) of at least 1 line.

38. The method of any one of the preceding claims, wherein the individual has an improvement in distance vision (e.g., visual acuity at 4 m) of at least 3 letters.

39. The method of any one of the preceding claims, wherein the individual is at least 60 years old.

40. The method of any one of the preceding claims, wherein the individual is 61-65 years old.

41. The method of any one of the preceding claims, wherein the individual is 66-70 years old.

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