Hydrogenophilus bacterium mutant having improved crotonyl-coa production capability
A genetically modified Hydrogenophilus bacterium efficiently produces crotyl alcohol, butadiene, and butanol from carbon dioxide, addressing greenhouse gas emissions and chemical production inefficiencies.
WO2025220580A1 Publication Date: 2025-10-23UTILIZATION OF CARBON DIOXIDE INST CO LTD +1
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Patent Information
- Application Number
- PCT/JP2025/014249
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-04-14
- Filing Date
- 2025-04-09
- Publication Date
- 2025-10-23
AI Technical Summary
Technical Problem
Current methods for producing chemicals like butadiene and butanol from petroleum are inefficient and contribute to greenhouse gas emissions, while using biomass for chemical production disrupts food and feed supplies and causes environmental destruction.
Method used
A mutant Hydrogenophilus bacterium with disrupted genes for enzymes that catalyze side reactions in the crotonyl-CoA pathway, allowing for high-efficiency production of crotyl alcohol, butadiene, and butanol using carbon dioxide as a carbon source.
Benefits of technology
The mutant bacterium addresses global warming by efficiently converting carbon dioxide into valuable chemicals and biofuels, meeting the demand for chemical products while reducing reliance on petroleum.
✦ Generated by Eureka AI based on patent content.
Abstract
Provided is a Hydrogenophilus bacterium mutant in which at least one of the below-mentioned enzyme genes (a) to (d) is disrupted, wherein the enzyme genes are located on the chromosome of a Hydrogenophilus bacterium and catalyze any one of reactions in the pathway for generating 3-hydroxybutyryl-CoA from crotonyl-CoA. The mutant is improved in terms of crotonyl-CoA production capability and can therefore be used for the efficient production of useful substances generated via crotonyl-CoA. (a) A gene that encodes a polypeptide having a 3-hydroxybutyryl-CoA dehydratase activity; (b) a gene that encodes a polypeptide having an enoyl-CoA hydratase activity; (c) a gene that encodes a polypeptide having a 3-hydroxyacyl-CoA dehydrogenase activity; and (d) a gene that encodes a polypeptide having a methylglutaconyl-CoA hydratase activity.
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