Pressing and sealing apparatus for POCT kit, and molecular diagnostic device and operation method

Abstract

The present invention relates to the technical field of medical instruments, and relates to the technical field of small amplification devices, especially portable amplification devices. Disclosed are a pressing and sealing apparatus for a POCT kit, and a molecular diagnostic device and operation method. The pressing and sealing apparatus for a POCT kit comprises a movable bracket, wherein a transmission assembly can drive the movable bracket to enable a sealing portion to seal a reaction chamber of the POCT kit when the movable bracket moves in a first direction; and when the transmission assembly drives the movable bracket to move in a second direction, a pressing portion comes into contact with the reaction chamber of the POCT kit to enable the reaction chamber of the POCT kit to tightly fit with a heat source. In the molecular diagnostic device including the pressing and sealing apparatus, a valve control portion and a clamping device share a second drive motor, a hot-melt bonding portion of the sealing portion and a detection and acquisition unit of the pressing portion share a first drive motor, a gas source drive part and a clamping apparatus cooperate with each other to drive liquid to flow through chambers of the kit, thereby achieving high integration, few drive parts, reliable operation, high detection accuracy, and small contamination.

Description

A compression sealing device for a POCT kit, a molecular diagnostic device and an operating method TECHNICAL FIELD

[0001] The present application relates to the technical field of small-scale amplification devices, particularly portable amplification devices that cooperate with POCT kits, and specifically relates to a compression sealing device for a POCT kit, a molecular diagnostic device and an operating method. BACKGROUND

[0002] In vitro diagnostic technology can separate and take out samples such as blood, body fluid, tissue fluid, etc. from a living body, and use in vitro detection reagents, kits, calibrators, quality control materials, etc. to test and verify the samples. The sub-application scenarios that have been developed include biochemical diagnosis, immunodiagnosis, molecular diagnosis, microbiological diagnosis, blood and body fluid diagnosis, and POCT, etc. From the application scenario, POCT has formed a special sub-type device with an increasing proportion in the method classification. Molecular POCT takes genetic material such as DNA or RNA sequence segments of a living body as the analysis object, and can realize timely and accurate detection of low-abundance targets in the early stage of pathogen (virus, bacteria, microorganism, etc.) invasion through amplification enrichment technology. The polymerase chain reaction (PCR) in molecular diagnosis has provided great convenience for medical and biological research since its invention in the 20th century by expanding the recognition and replication of genetic material such as DNA. It is now widely used in genetic disease detection, genetic fingerprinting, infectious disease diagnosis, gene cloning, parentage testing and DNA computing. With the rise of the PCR instrument, an automatic device that can realize genetic material amplification detection, especially the POCT diagnostic device closer to the patient, can be used for diagnosis in a doctor's office, hospital emergency room, workplace, pharmacy or even home, thereby realizing rapid diagnosis of pathogens. Designing a molecular POCT diagnostic device is the development direction of future precision medicine.

[0003] With the increasing prevalence of various diseases, especially large-scale epidemics, more and more companies are dedicated to developing miniaturized, highly integrated diagnostic devices. Some well-known American companies, such as IDNOW and Cue Health, have designed products based on isothermal amplification technology. However, in recent years, the primer and probe design for isothermal amplification technology has proven challenging, and the detection sensitivity of these products is insufficient. In contrast, GeneXpert and FilmArray, based on cyclic heating and cooling technology, have seen faster growth in installations in the past two years, demonstrating the stability and high sensitivity of thermal cycling amplification technology. From a design perspective, these two products are relatively large, and in actual use, they are often deployed in multi-device configurations, which limits their application in primary healthcare settings. Both patents with publication number CN116328860A and US11679387B2 disclose an integrated rotary valve scheme, which limits the rotary valve to be driven to two different valve positions to connect different chambers and flow channels, thereby driving the fluid to flow in different chambers to complete the integrated design scheme of extraction and detection. This type of equipment requires high precision in the machining of the rotary valve and in its assembly, resulting in high costs and significant manufacturing challenges. Patent EP4190890A1 discloses an integrated testing consumable that uses a sealed film to encapsulate a reagent kit containing various processing reagents, ensuring unobstructed airflow through the chambers after the sealed film is punctured, and using a stopper rod to drive the liquid movement to different chambers. Patent EP3741839B1 discloses a solution that uses two sets of gas sources alternately driven at different positions, allowing the amplified solution to alternately move between two different target temperature zones for amplification. A fluorescence detection unit is placed in the flow channel between the two temperature zones to obtain the amplification results. This solution places extremely high demands on the sealing of the consumables and the driving mechanism. High precision is required. Patents US11904314B2 and US11904315B2 disclose solutions that address the need for sealing the amplification solution during the amplification process, leading to a wax valve solution. This solution melts wax valves in different chambers at high temperatures, connecting them and allowing the melted wax to enter the amplification reaction chamber along with the amplification solution, thus covering the surface of the reaction solution. Patent CN117417819A replaces the widely used fluorescence detection with an electrode detection solution. While this may reduce manufacturing costs, the system struggles to achieve multi-target detection. Furthermore, with increasing detection requirements, this solution is extremely difficult to meet the demands for obtaining quantitative results. Although various POCT devices have been designed in the prior art, these devices are typically not highly integrated, have complex system structures, and require high precision in the manufacturing and assembly of core components. Therefore, targeted optimization of integrated detection devices is essential.

[0004] In a specific scenario, the POCT kit includes a substrate, a thin film covering the front and back of the substrate, wherein the substrate is provided with a sample addition hole, a flow channel, and a plurality of parallel PCR holes, and the hole wall of the PCR hole, the front film and the back film constitute a reaction cavity. When the POCT kit is placed in the nucleic acid detector, the reaction cavity (usually an amplification reaction cavity) in the POCT kit needs to be heated, lighted, detected, etc. Therefore, it is necessary to ensure that the reaction area of the POCT kit and the heating surface of the heating element are in close contact to achieve efficient heat transfer. However, due to the flexible thin film of the PCR reaction cavity front and back film, the amount of fluid entering the reaction cavity is not uniform, and the difference in surface flatness of the reaction cavity makes the heat not evenly transmitted to each reaction cavity, affecting the final reaction efficiency. In addition, in order to ensure that the fluid does not leak, the sealing effect of the equipment is also very important. The Chinese utility model with publication number CN218561421U discloses an automatic pressing device of a microfluidic chip and a nucleic acid detector, which comprises a chip bin and an automatic pressing device. With the insertion of the microfluidic chip, the convex part of the microfluidic chip and the trigger end of the pressing mechanism are in close contact, so that the pressing mechanism rotates around the rotating shaft, and the spring of the pressing mechanism is pressed to the heating surface of the heating element, so that the heating surface can be tightly attached to the heating area of the microfluidic chip. The pressing device can rely on the rotation of the rotating shaft to press the microfluidic chip with the spring pressing surface. However, this structure has only one function, which can only realize the pressing of the POCT kit, and it is difficult to realize the sealing of the reaction cavity of the POCT kit. At the same time, the pressure of the spring is limited, and there is a situation that part of the reaction cavity is not evenly heated due to the uneven surface. The Chinese utility model with publication number CN219951034U discloses a chip locking mechanism for molecular diagnostic equipment, which comprises a bottom frame, a moving side frame and a fixed side frame, and a moving side link, a fixed side link and a cross link. The moving side link is hinged to the moving side frame, the fixed side link is hinged to the fixed side frame, and the cross link is hinged to the moving side link and the fixed side link, and moves up and down through the cross link to press and release the chip. However, the device has a complex structure, only one function, high manufacturing cost, and poor stability of the locking mechanism.

[0005] The detection system also has a greater impact on the POCT type diagnostic device. Chinese invention CN117025379B authorizes a RAPID constant temperature amplification nucleic acid detection device and detection method. In this scheme, the sample in the sampling tube is added and processed in the processing cavity, and then the processed amplification system liquid is heated to perform amplification. Finally, the amplified liquid is guided into the chromatographic type colloidal gold detection part. This design saves the fluorescence quantitative module and cannot realize quantitative detection, and the detection precision and sensitivity are relatively low. The stability of the constant temperature amplification primer probe design is more complex and lower. Chinese invention CN117286017A discloses a multi-channel nucleic acid amplification analysis device. The transmitting end and the receiving end are arranged in a 90° cross type at the bottom and side of the consumable receiving hole. This device is practical for small pure amplification devices, but it is difficult to use for integrated extraction and amplification devices. US20230416811A1 discloses a device for applying thermal energy to a container and detecting emission signals from the container. In this scheme, the optical fiber is inserted from the bottom of the consumable receiving part, and the inserted optical fiber is exposed to the bottom area of the receiving part. The heat cycle heating part is arranged on the side of the receiving part. Changing the position of the heating part can make the optical fiber closer to the consumables inside the consumable receiving part. This scheme is also difficult to apply to small amplification devices. US6369893B1 authorizes a flat amplification consumable corresponding optical detection scheme, which configures a flat surface as a thermal cycle contact surface, so that the liquid in the consumable has a higher heat transfer surface. The thickness of the flat amplification consumable is configured with two separately arranged transmitting ends and receiving ends, and can be configured as a multi-channel configuration to achieve multiple detection effects. This scheme has specific requirements for the form of consumables. PCT application WO2023248185A1 authorizes a compact detection system. In this scheme, the transmitting light end and the receiving light end are independently arranged on the same side of the detected chamber, and the transmitting light is emitted through the optical fiber. The two transmitting light ends can share one receiving end. The common receiving end optical fiber is connected to a specific part to form a compact detection system. Chinese invention CN110967324A discloses an optical detection device of a multi-channel real-time fluorescence detector. In this scheme, each transmitting optical fiber and receiving optical fiber is arranged at a predetermined angle, and a microlens array is arranged at the receiving end to improve detection efficiency. This scheme mainly optimizes the layout of the transmitting end and the receiving end to achieve the highest efficient detection target.

[0006] In order to ensure that the POCT kit used by the small amplification device can accurately perform amplification, it is urgent to design a low-cost solution with simple sealing structure and effective and reliable corresponding configuration structure. Similar problems also include: most kits are difficult to perform multiple detection of multiple targets, the devices capable of performing single-person multiple target detection on the market are very large in size, and the processing cost is high and the processing precision requirement is also high, and a kit with low cost, simple processing and low precision requirement and capable of performing multiple detection needs to be designed; the POCT kit needs more flow control valves and a more complex rotary control method, and there are many related theoretical researches, although there are some mature products, but the low-cost implementation depends on the improvement of the processing technology maturity of the rotary disc, and the electric immersion as a new driving scheme has very high research value, but it is very difficult to realize low-cost detection in the short term or even in the medium term, and a low-cost, effective and reliable driving force design is needed. SUMMARY

[0007] The purpose of the present application is to provide a compression sealing device for a POCT kit, a molecular diagnostic device and an operating method, and to provide a compression sealing device for a POCT kit, which includes a moving support, a transmission assembly capable of driving the moving support, so that when the moving support moves in a first direction, the sealing part seals the reaction cavity of the POCT kit; when the transmission assembly drives the moving support to move in a second direction, the compression part contacts the reaction cavity of the POCT kit, so that the reaction cavity of the POCT kit is tightly attached to the heat source.

[0008] In addition, the molecular diagnostic device comprising the compression sealing device shares a second driving motor with the clamping device, the hot melt joint part of the sealing part shares a first driving motor with the detection and collection unit, the liquid in the chambers of the kit is driven to flow by the cooperation of the distribution driving part and the clamping device, which has high integration, few driving parts, reliable operation, high detection accuracy and low pollution.

[0009] The technical scheme adopted by the present application is as follows:

[0010] The first aspect of the present application discloses a compression sealing device for a POCT kit, which comprises a fixed clamping part, a compression part, a sealing part and a transmission assembly, wherein,

[0011] The fixed clamping part comprises a kit receiving site and a moving support, the kit receiving site can clamp the POCT kit, the transmission assembly can drive the moving support to move in a first direction or a second direction, so that the moving support drives the compression part and the sealing part to alternately contact or move away from the POCT kit, the sealing part seals the reaction cavity of the POCT kit when it contacts the POCT kit, and the compression part makes the reaction cavity of the POCT kit tightly attached to the heat source when it contacts the POCT kit.

[0012] Further, the transmission assembly is movably connected with the pressing part and the sealing part respectively, the pressing part is movably connected with the moving support, and the sealing part is movably connected with the moving support.

[0013] Further, the fixed clamping part further comprises a fixed table, a first guide rod and a second guide rod, the fixed table is above the moving support, the fixed table is connected with the clamping part, the first guide rod and the second guide rod both penetrate through the fixed table and are movably connected with the fixed table, one end of the first guide rod close to the POCT kit is connected with the sealing part, one end of the second guide rod close to the POCT kit is connected with the pressing part, when the moving support moves along the first direction, the sealing part can move along the first guide rod to approach the POCT kit, and the pressing part can move along the second guide rod to move away from the POCT kit, when the moving support moves along the second direction, the sealing part can move along the first guide rod to move away from the POCT kit, and the pressing part can move along the second guide rod to approach the POCT kit.

[0014] Further, the fixed clamping part further comprises a mounting plate, a first rotating rod, a second rotating rod, a first connecting rod and a second connecting rod, the mounting plate is fixed on the transmission assembly, the mounting plate is provided with columns, the columns are distributed on both sides of the moving support, the first connecting rod penetrates through the first connecting holes on the columns on both sides of the moving support, the second connecting rod penetrates through the second connecting holes on the columns on both sides of the moving support, the moving support is provided with a pressing part movable hole and a sealing part movable hole, the second rotating rod penetrates through the pressing part movable hole, and the first rotating rod penetrates through the sealing part movable hole, both sides of the moving support are symmetrical in structure, on the first side of the moving support, the first connecting rod is movably connected with the first rotating rod, the first rotating rod is movably connected with the sealing part, the second connecting rod is movably connected with the second rotating rod, and the second rotating rod is movably connected with the pressing part, when the transmission assembly drives the moving support to move, the first rotating rod drives the first connecting rod to move, the sealing part moves away from or approaches the POCT kit, the second rotating rod drives the second connecting rod to move, and the pressing part approaches or moves away from the POCT kit.

[0015] Further, the first connecting rod and the first rotating rod are hinged to form a first connecting part, the first rotating rod and the sealing part are hinged to form a second connecting part, the included angle between the first connecting part and the second connecting part in the clockwise direction ranges from 85° to 180°, the second connecting rod and the second rotating rod are hinged to form a third connecting part, the second connecting rod and the pressing part are hinged to form a fourth connecting part, and the angle between the third connecting part and the fourth connecting part in the counterclockwise direction ranges from 85° to 180°.

[0016] In a second aspect, the present application further discloses a molecular diagnostic device comprising the compact sealing device for POCT kit disclosed in the first aspect, wherein the POCT kit comprises a buffer cavity for receiving an external liquid, a system cavity for dispensing the liquid, an amplification cavity for performing detection, and a liquid distribution driving part for controlling the air pressure in the amplification cavity, the buffer cavity and the amplification cavity are in fluid communication with the system cavity, the system cavity is in communication with the external environment through a back gas port, a flow control valve is arranged between the buffer cavity and the amplification cavity to control the on-off relationship, and the liquid distribution driving part can be elastically deformed under the action of an external force and restored without the action of the external force.

[0017] The device body comprises a plurality of kit receiving parts for receiving POCT kits and performing amplification detection, each kit receiving part comprises a kit receiving position, a clamping device for adjusting the pressure of the system cavity to control the on-off relationship between the system cavity and the amplification cavity, a valve control part for controlling the on-off relationship of the flow control valve, and a liquid distribution driving part for communicating and controlling the air pressure in the buffer cavity; the valve control part and the clamping device are movably connected to the device body, the valve control part can be moved to the position of the flow control valve corresponding to the kit receiving position and can drive the flow control valve to open, and can be moved away from the kit receiving position to close the flow control valve, the clamping device can be moved to the position of the liquid distribution driving part corresponding to the kit receiving position and can press the liquid distribution driving part to increase the air pressure in the amplification cavity, and can be moved away from the kit receiving position to reduce the air pressure in the amplification cavity; at least part of the valve control part and the clamping device coincide when moving.

[0018] Further, the clamping device can press the liquid distribution driving part under the action of the second driving part, and the valve control part is connected to and follows the clamping unit through a transmission block.

[0019] Further, the clamping device comprises a pair of clamping units, which are oppositely arranged on both sides of the kit receiving position, and can be driven by the second driving part to approach each other and press the liquid distribution driving part from both sides in the thickness direction of the POCT kit; the second driving part comprises a second driving motor and a transmission screw, the transmission screw is provided with a right-handed thread segment and a left-handed thread segment, and the pair of clamping units are respectively threadedly connected to the right-handed thread segment and the left-handed thread segment of the transmission screw.

[0020] Further, the kit receiving part further comprises a thermal cycling part for amplifying the POCT kit, and a detection part for detection, and the detection collection units of the thermal cycling part and the detection part are respectively arranged on the opposite sides of the kit receiving position.

[0021] Further, the air source driving part comprises an air source driving member and an air source connecting pipe, the buffer cavity is communicated with the air source connecting pipe through an air inlet; the kit receiving part is provided with a receiving opening for receiving the POCT kit, a closable receiving cover is arranged at the receiving opening, an air source interface is arranged on the receiving cover, one side of the air source interface is in communication with the air source connecting pipe, and the other side is matched with the air inlet of the POCT kit in the kit receiving position, and a rubber pad for sealing and pressing the air inlet is arranged on the side of the air source interface matched with the air inlet.

[0022] Further, the sealing part further comprises a hot melt joint part for destructively closing the amplification cavity, the device body is provided with a device air inlet at the front end and a device air outlet at the rear end, the thermal cycle part is arranged on one side close to the device air outlet, and the hot melt joint part and the detection and collection unit are arranged on one side close to the device air inlet.

[0023] Further, the detection module, and the transceiving detection module are further included, wherein the sealing part comprises a hot melt joint part, the hot melt joint part seals the amplification cavity of the POCT kit when the hot melt joint part is in contact with the POCT kit, the pressing part is further connected to the detection module, the detection module is provided with a detection optical fiber, and the detection module is in contact with the POCT kit, so that the reaction cavity of the POCT kit is tightly attached between the first end of the detection optical fiber and the device heat source, the second end of the detection optical fiber is connected to the transceiving detection module, the transceiving detection module comprises a multi-channel transceiving unit capable of being driven to move, and the multi-channel transceiving unit scans the second end of the detection optical fiber to obtain real-time fluorescence results in the reaction cavity.

[0024] Further, the detection module comprises a plurality of protruding detection units, the detection unit further comprises a detection hole penetrating through a columnar protruding structure, and the detection hole is configured to be connected to the detection optical fiber.

[0025] Further, the fiber optic restraint part is further included, the fiber optic restraint part comprises a limiting plate fixedly connected to the detection module, and a clamping plate matched with the limiting plate, a flexible interlayer is arranged between the limiting plate and the clamping plate, and the detection optical fiber is arranged in the flexible interlayer.

[0026] Further, the limiting plate is in a bent configuration, the bent configuration comprises a connecting part fixedly connected to the detection module, and a limiting part matched with the clamping plate, the middle section of the limiting part and the middle section of the connecting part are bent at an angle in the range of 55° to 85°, and the material of the flexible interlayer is sponge, and the density of the sponge is 20 to 28 kg / m 3 .

[0027] Further, the transceiving detection module comprises a transceiving substrate, a transceiving driving mechanism, a transceiving driving board and a multi-channel transceiving unit, the transceiving substrate is provided with a fiber fixing hole, the second end of the detection fiber is connected to the fiber fixing hole, the transceiving driving mechanism is arranged on one side of the transceiving substrate in the thickness direction, the transceiving driving mechanism comprises a transceiving driving motor, the transceiving driving motor is connected with a transceiving driving wheel, a transceiving transmission wheel is arranged at a preset interval from the transceiving driving wheel, a transceiving transmission belt is arranged around the transceiving driving wheel and the transceiving transmission wheel, one side of the transceiving transmission belt is fixedly connected with a transceiving transmission block, the transceiving transmission block is also embeddedly connected to a transceiving slide rail fixedly arranged on the transceiving substrate, the transceiving transmission block is also connected with the transceiving driving board and the multi-channel transceiving unit, and the transceiving driving motor can drive the multi-channel transceiving unit to slide, so that the multi-channel transceiving unit can move through and scan the fiber fixing hole.

[0028] Further, it also comprises a thermal cycle part, which comprises a local heat dissipation device including a base, a heat conduction component, and a heat dissipation component, wherein one side of the base is fixed with a heat source, and the other side of the base is connected with the heat conduction component.

[0029] The heat conduction component comprises a heat transfer base and a heat pipe heat exchanger, the heat transfer base is connected with the base for heat transfer, and the heat pipe heat exchanger comprises a group of pipe groups or multiple groups of pipe groups arranged at intervals, each group of pipe groups comprises a heat conduction part, a first heat dissipation part and a second heat dissipation part, the heat conduction part is embedded in the heat transfer base, the first heat dissipation part penetrates through the heat dissipation fin group along the length direction of the heat dissipation fin group and is connected with the first end of the heat conduction part, and the second heat dissipation part penetrates through the heat dissipation fin group along the length direction of the heat dissipation fin group and is connected with the second end of the heat conduction part; the penetrating direction of the first heat dissipation part in the heat dissipation fin group is different from the penetrating direction of the second heat dissipation part in the heat dissipation fin group; and the heat dissipation component comprises a fin heat dissipation unit and a fan.

[0030] Further, the first end of the bracket is fixed with the heat dissipation fin group, the second end of the bracket is fixed with the fan, a plurality of heat dissipation fins are arranged along the length direction of the heat dissipation fin group, the heat pipe heat exchanger penetrates through the plurality of heat dissipation fins, and the fan is located in a straight line position with the heat dissipation fin group and the heat source, so that the fan can directly dissipate heat for the heat dissipation fin group and the heat source.

[0031] In a third aspect, the application also discloses an operating method of a molecular diagnostic device, which uses the molecular diagnostic device disclosed in the second aspect and comprises the following steps:

[0032] A pre-operation step: placing a POCT reagent box in a reagent box receiving position of the molecular diagnostic device;

[0033] Driving preparation step: the driving clamping device moves to the POCT reagent kit until the clamping device presses the dispensing driving part, the dispensing driving part produces elastic deformation, the gas pressure in the amplification cavity increases, the liquid is limited to flow from the system cavity into the amplification cavity, the valve control part is close to and acts on the flow control valve, the flow control valve is opened, the buffer cavity is communicated with the system cavity, and at least part of the clamping device and the valve control part coincide during movement;

[0034] Gas source driving step: the gas source driving part is started, the gas pressure in the buffer cavity is changed, the liquid flows between the buffer cavity and the system cavity, and after the liquid is fully mixed, the liquid is stored in the system cavity;

[0035] Dispensing driving step: the clamping device is driven to release the dispensing driving part, the dispensing driving part gradually recovers to produce negative pressure in the amplification cavity, and the liquid in the system cavity is driven to flow into the amplification cavity under the action of the negative pressure until the filling of the amplification cavity is completed.

[0036] Further, it further comprises an amplification detection step: the thermal cycle part is started to apply constant temperature or variable temperature to the amplification cavity, so that the liquid in the amplification cavity produces amplification reaction, and the detection part drives the amplification system liquid in the amplification to perform fluorescence detection to obtain amplification detection results.

[0037] As described above, due to the adoption of the above technical scheme, the beneficial effects of the present application are:

[0038] 1. The compression sealing device for the POCT reagent kit provided by the present application, through the cooperative action of the moving bracket, the first rotating rod, the second rotating rod, the first connecting rod, the second connecting rod, the compression part and the sealing part, the device integrates sealing and compression, can not only realize the sealing function of the POCT reagent kit reaction cavity, but also can realize the full compression function of the reaction cavity, and avoids the uneven heating condition in amplification. In addition, by arranging the first connecting rod and the second connecting rod capable of adjusting the included angle, the compression part and the sealing part can obtain a large enough extrusion force in the horizontal direction, and a mutually independent boss is designed on the compression head to increase the contact area of the compression head and the reaction cavity, thereby avoiding the occurrence of the uneven heating condition of the reaction cavity caused by the difference in the flatness of the reaction cavity surface and the insufficient extrusion force, improving the sealing performance of the PCR reaction cavity, and strengthening the close contact between the PCR reaction cavity and the heating surface, thereby improving the reaction efficiency of the reaction cavity of the POCT reagent kit and greatly improving the work efficiency.

[0039] 2. The compression sealing device for the POCT reagent kit provided by the present application, by arranging the first rotating rod, the second rotating rod, the first connecting rod, the second connecting rod, the compression part and the sealing part, the device integrates the sealing and compression functions, has a simple structure, and can automatically seal and compress the POCT reagent kit, is simple to operate, saves time and effort, has good compatibility, has low manufacturing cost, short production cycle, high safety factor, and wide application prospect.

[0040] 3. The application provides a compression sealing device for a POCT kit, wherein the valve control part shares a second driving motor with the clamping device, the hot melt bonding part of the sealing part shares a first driving motor with the detection and collection unit, the air source driving part and the clamping device drive the liquid to flow in each chamber of the POCT kit in cooperation, and the compression sealing device has high integration, few driving parts, reliable operation, high detection accuracy and small pollution.

[0041] 4. The clamping device of the application increases the air pressure in the amplification cavity when the compression and separation driving part is compressed, avoids the liquid in the system cavity from entering the amplification cavity, and when the compression and separation driving part is loosened, the deformation of the compression and separation driving unit recovers to reduce the air pressure in the amplification cavity to form a suction effect, so that the liquid in the system cavity can be sucked into the amplification cavity.

[0042] 5. The valve control part of the application can follow the clamping device to realize the connection between the buffer cavity and the system cavity when the amplification cavity and the system cavity are closed, and the connection between the amplification cavity and the system cavity when the buffer cavity and the system cavity are closed.

[0043] 6. The hot melt bonding part of the application can hot melt and destructively close the amplification cavity, reliably ensure the pressure in the amplification cavity, and form a low-pressure leakage or even no-pressure leakage state similar to a pressure cooker, so that the amplification system liquid can avoid generating bubbles in high and low temperature cycle amplification, and the destructive closing operation also makes multiple amplification cavities in an independent closed state, and almost no steam and mutual interference problems occur.

[0044] 7. The clamping device of the application comprises a pair of clamping units arranged in pairs and acts on the separation driving part in a two-side compression manner, compared with the single-side compression scheme, the two-side compression makes the film of the separation driving part have a smaller deformation amount when being compressed, and the pressure adjustment range is larger.

[0045] 8. The fluorescence detection module of the present application receives the POCT kit through the kit receiving position, and the heat transfer connection of the heat cycle part is made on one side of the POCT kit in the thickness direction, and the other side can abut against the detection module and the hot melt combination part, when the composite driving mechanism of the hot melt combination part and the detection module drives the moving support to move in the first direction or the second direction, the hot melt combination part and the detection module are alternately moved horizontally to the position of the reaction cavity, so that the hot melt combination part can seal the fluid channel connected with the reaction cavity in the POCT kit, and form a mutually independent and sealed reaction cavity, when the composite driving mechanism drives the moving support to move in the opposite direction, the detection module moves to the reaction cavity of the POCT kit and applies horizontal pressure, so that the reaction cavity of the POCT kit is closely attached to the heat source, avoiding the uneven heating of the reaction cavity caused by the difference in surface flatness and insufficient extrusion force, improving the sealing performance of the PCR reaction cavity, and improving the efficiency of the PCR reaction by closely contacting the PCR reaction cavity with the heating surface.

[0046] 9. The fluorescence detection module provided by the present application changes the way of fixing and installing the detection optical fiber, and the first end of the detection optical fiber is arranged on the detection module which can approach or move away from the reaction cavity of the POCT kit, so that the detection optical fiber can follow the detection module to perform amplification fluorescence detection, and the detection module can press the reaction cavity to perform efficient detection, the second end of the detection optical fiber is connected to the transceiving detection module, and the multi-channel transceiving unit can be scanned to perform multi-target detection, the light fiber restraint part is arranged, the limiting plate is arranged in a bending structure and bent at a specific angle, the detection module is fixedly connected to the limiting plate, the clamping plate matched with the limiting plate is arranged, the flexible clamping layer is arranged between the limiting plate and the clamping plate, and the detection optical fiber is arranged in the flexible clamping layer, so that even if the detection module moves, the light path and transmission efficiency of the detection optical fiber will not be affected, the limiting plate with the bending angle can maximize the change of the direction of the detection optical fiber, and the reliability of use is ensured without damaging the optical fiber, the detection optical fiber is fixed in the flexible clamping layer, especially in the multi-layer sponge clamping layer, so that the detection optical fiber can be reliably fixed without the risk of extrusion deformation, the reliable fixation of the detection optical fiber is realized, the second end of the detection optical fiber is inserted into the optical fiber fixing hole arranged in a line on the transceiving substrate, and the multi-channel transceiving unit is driven by the transceiving driving mechanism to move and traverse each optical fiber fixing hole, so that efficient and rapid detection is realized, and the closed multi-channel transceiving unit can shield the interference of external stray light, so that the detection result is accurate and reliable.

[0047] 10. The method for operating the molecular diagnostic device provided by the application is suitable for the molecular diagnostic device described above, can perform amplification detection operation using the POCT kit, and can drive the valve control part and the clamping device synchronously in the overlapping time period in the amplification, so that the overall operation control is simple and efficient, the air source driving part and the liquid distribution driving part can serve as the power source in different stages such as system liquid transfer and amplification liquid distribution, so that the differentiated requirements for the transfer liquid amount and the transfer precision can be met synchronously. BRIEF DESCRIPTION OF DRAWINGS

[0048] Fig. 1 is a schematic diagram of the overall structure of the compression sealing device for the POCT kit provided by the application;

[0049] Fig. 2 is another schematic diagram of the structure of the compression sealing device for the POCT kit provided by the application;

[0050] Fig. 3 is a schematic diagram of the side view structure of the compression sealing device for the POCT kit provided by the application cooperating with Fig. 2;

[0051] Fig. 4 is an exploded view of the structure of the compression sealing device for the POCT kit provided by the application;

[0052] Fig. 5 is a schematic diagram of the structure of the transmission assembly in the compression sealing device for the POCT kit provided by the application;

[0053] Fig. 6 is a schematic diagram of the structure of the compression sealing device for the POCT kit provided by the application before operation;

[0054] Fig. 7 is a schematic diagram of the structure of the compression sealing device for the POCT kit provided by the application in the compression part;

[0055] Fig. 8 is a schematic diagram of the state of the compression sealing device for the POCT kit provided by the application when performing sealing work;

[0056] Fig. 9 is a schematic diagram of the state of the compression sealing device for the POCT kit provided by the application when performing compression work;

[0057] Fig. 10 is a structure diagram of the sample tube which can be used in cooperation with the POCT kit provided by the application;

[0058] Fig. 11 is a structure diagram of the POCT kit provided by the application;

[0059] Fig. 12 is a structure diagram of the molecular diagnostic device cooperating with the POCT kit;

[0060] Fig. 13 is a state diagram of the POCT kit put into the molecular diagnostic device provided by the application;

[0061] Fig. 14 is a functional component distribution diagram of the POCT kit put into the device provided by the application;

[0062] Figure 15 is a functional component distribution diagram of the POCT kit after being put into the device from another perspective;

[0063] Figure 16 is a layout diagram of a high-throughput detection scheme provided by the present application in a two-parallel configuration;

[0064] Figure 17 is a side sectional view of the POCT kit provided by the present application;

[0065] Figure 18 is a side sectional view of the valve control part and the clamping device of the present application being synchronously driven to act on the integrated detection consumable;

[0066] Figure 19 is a structural schematic diagram of the valve control part and the clamping device of the present application sharing a second driving motor;

[0067] Figure 20 is a schematic diagram of the valve control part and the clamping device of the present application acting on the POCT kit;

[0068] Figure 21 is a structural schematic diagram of the air source driving member being connected to the buffer cavity in the open state of the flow control valve of the present application;

[0069] Figure 22 is a structural diagram of the layout of the air source driving member and the driving motor of the present application;

[0070] Figure 23 is a schematic diagram of the state of the liquid being driven by the air source driving member to flow between the system cavity and the buffer cavity in the open state of the flow control valve of the present application;

[0071] Figure 24 is a diagram of the state of the liquid being stored in the system cavity of the POCT kit of the present application;

[0072] Figure 25 is a diagram of the state of the liquid in the system cavity of the POCT kit of the present application being driven by the negative pressure of the liquid distribution driving part to be distributed to the amplification cavity;

[0073] Figure 26 is a diagram of the state of the first driving motor driving the hot melt bonding part to close the amplification cavity of the present application;

[0074] Figure 27 is a diagram of the state of the hot melt bonding part moving away from the POCT kit after the completion of the fusion of the present application;

[0075] Figure 28 is a sectional view of the detection part compressing the amplification cavity and cooperating with the thermal cycling part to complete the amplification detection of the present application;

[0076] Figure 29 is a structural schematic diagram of the transceiving detection unit of the present application;

[0077] Figure 30 is a schematic diagram of the connection structure of the optical fiber restraining part and the detection module in the fluorescence detection module provided by the present application;

[0078] Figure 31 is an exploded schematic diagram of the optical fiber restraining part in the fluorescence detection module provided by the present application;

[0079] Figure 32 is a schematic diagram of the structure of the transceiving detection module in the fluorescence detection module provided by the present application;

[0080] Figure 33 is a schematic diagram of the driving of the multi-channel transceiving unit in the transceiving detection module of Figure 32 to traverse the fixed hole end of the detection optical fiber;

[0081] Figure 34 is a schematic diagram of the structure of the cyclic amplification detection module provided by the present application;

[0082] Figure 35 is a schematic diagram of the structure of the thermal cycle unit provided by the present application;

[0083] Figure 36 is an exploded view of the thermal cycle unit corresponding to Figure 35;

[0084] Figure 37 is a schematic diagram of the structure of the thermal cycle unit from one perspective;

[0085] Figure 38 is a schematic diagram of the structure of the thermal cycle unit from another perspective.

[0086] Marked in the figure: 10-POCT kit, 11-probe module, 110-probe unit, 100-card mounting part, 1000-sample tube, 101-mounting plate, 1010-box cover, 1011-stem, 1012-guide rail slider, 102-fixing table, 103-first guide rod, 104-second guide rod, 105-limiting ring, 106-photoelectric sensor, 107-moving support, 1071-pressing part movable hole, 1072-sealing part movable hole, 108-first rotating rod, 109-second rotating rod, 110-first connecting rod, 1101-first connecting part, 111-air inlet, 112-gas return port, 1102-second connecting part, 12-buffer cavity, 120-second connecting rod, 1201-third connecting part, 1202-fourth connecting part, 123-flow control valve, 13-system cavity, 14-amplification cavity, 15-liquid separation driving part, 1610-bonding auxiliary part, 17-first flow channel, 18-second flow channel; 2-device body, 200-pressing part, 2000-middle base plate, 201-reagent kit receiving position, 2020-control part, 203-device air inlet, 2019-pressing head seat, 2029-pressing head, 2001-first reagent kit receiving part, 2002-second reagent kit receiving part, 2021-boss, 2022-fiber hole, 21-valve control part, 211-magnet, 22-alternating transmission mechanism, 23-thermal cycle part, 2321-bracket, 231-radiation fan, 232-finned heat dissipation unit, 2320-radiation fin group, 233-heating part, 2331-heat-conducting mounting plate, 2332-heat-conducting base, 234-heat pipe heat exchanger, 24-clamping part, 241-second driving motor, 242-transmission screw rod, 243-transmission guide rail, 25-air source driving piece, 26-detection and collection unit, 261-contact part, 2611-detection optical fiber, 28-multichannel transceiver end, 280-fiber fixing hole, 29-fiber restraint part, 291-limiting plate, 292-flexible interlayer, 293-clamping plate; 30-receiving cover, 300-sealing part, 301-hot melt bonding part, 3010-buckle, 31-air source interface, 32-box cover containing part; 41-second fiber restraint part, 401-first driving motor, 402-base, 403-nut, 404-mounting seat, 405-screw rod. DETAILED DESCRIPTION

[0087] The application will be described in further detail below with reference to the drawings.

[0088] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application will be further described in detail below with reference to the drawings and examples. It should be understood that the specific examples described herein are only used to explain the present application and do not limit the present application.

[0089] The foregoing and other technical contents, features and effects of the present application can be clearly presented in the following detailed description of specific embodiments with reference to the accompanying drawings. Through the description of the specific embodiments, the technical means and effects taken by the present application to achieve the predetermined purposes can be understood more deeply and specifically. However, the accompanying drawings are provided for reference and illustration only, and are not intended to limit the technical solutions of the present application.

[0090] It should be noted that, in this paper, relational terms such as first and second are used merely to distinguish one entity or operation from another, and do not necessarily require or imply that there is any such actual relationship or order between these entities or operations. Moreover, the terms "include", "contain" or any other variants are intended to cover non-exclusive inclusion, so that the article or device including a series of elements not only includes those elements, but also includes other elements not explicitly listed. Without more limitations, the element defined by the statement "including a" does not exclude the presence of other identical elements in the article or device including the element.

[0091] Embodiment 1

[0092] Please refer to FIG. 1 and FIG. 2, FIG. 1 is the overall structure schematic diagram of the compression sealing device for POCT reagent kit provided by the present application, and FIG. 2 is the partial structure schematic diagram of the compression sealing device for POCT reagent kit provided by the present application. The compression sealing device for POCT reagent kit comprises a fixed clamping part, a compression part 200, a sealing part 300 and a transmission assembly.

[0093] The fixed clamping part comprises a clamping part 100 and a moving support 107, the clamping part 100 contains a reagent box receiving site 201 capable of clamping a POCT reagent box, and a transmission assembly capable of driving the moving support 107 to move in a first direction or a second direction, so that the moving support 107 drives the pressing part 200 and the sealing part 300 to alternately contact or move away from the POCT reagent box. In one case, the sealing part 300 contains a hot melt bonding part 301, so that when the hot melt bonding part 301 contacts the POCT reagent box, the hot melt seals the reaction cavity of the POCT reagent box, and when the pressing part 200 contacts the POCT reagent box, the reaction cavity of the POCT reagent box is tightly attached to the heat source. For example, when the transmission assembly drives the moving support 107 to move in the first direction, the moving support 107 drives the pressing part 200 to move away from the POCT reagent box, and at the same time, the moving support 107 drives the sealing part 300 to move close to and contact the POCT reagent box. When the transmission assembly drives the moving support 107 to move in the second direction, the moving support 107 drives the pressing part 200 to move close to and contact the POCT reagent box, and at the same time, the moving support 107 drives the sealing part 300 to move away from the POCT reagent box. Since the sealing part 300 and the pressing part 200 are time staggered to move close to or away from the POCT reagent box, when the sealing part 300 contacts the POCT reagent box, the hot melt or welding seals the reaction cavity of the POCT reagent box, avoiding the influence of the pressing part 200, and when the pressing part 200 contacts the POCT reagent box, the reaction cavity of the POCT reagent box is tightly attached to the heat source, also avoiding the influence of the sealing part 300.

[0094] Further, the transmission assembly is movably connected with the pressing part 200 and the sealing part 300 respectively, the pressing part 200 is movably connected with the moving support 107, and the sealing part 300 is movably connected with the moving support 107.

[0095] Preferably, the transmission assembly is a member with a hinged connection, or the transmission assembly is a cam structure, or the transmission assembly is a crank and connecting rod structure, etc.

[0096] In the embodiment, the fixed clamping part comprises a clamping part 100 and a moving support 107, the mounting plate 101 is hingedly connected with the moving support 107, the reagent box receiving position of the clamping part 100 can be inserted to receive and clamp the POCT reagent box. The pressing part 200 is hingedly connected with the moving support 107, specifically, the pressing part 200 is arranged opposite to the POCT reagent box and is hingedly connected with the moving support 107. The sealing part 300 is hingedly connected with the moving support 107, specifically, the sealing part 300 is arranged opposite to the POCT reagent box and is hingedly connected with the moving support 107. The transmission assembly can drive the moving support 107 to move in the first direction or the second direction. When the transmission assembly drives the moving support 107 to move in the first direction, the pressing part 200 moves away from the POCT reagent box, the sealing part 300 moves to the position of the reaction cavity of the POCT reagent box, and the sealing part 300 heat-fuses or welds to seal the reaction cavity of the POCT reagent box; when the transmission assembly drives the moving support 107 to move in the second direction, the sealing part 300 moves away from the POCT reagent box, the pressing part 200 moves to the position of the reaction cavity of the POCT reagent box, and the pressing part 200 contacts and presses the reaction cavity of the POCT reagent box, so that the reaction cavity of the POCT reagent box is tightly attached to the heat source. Since the sealing part 300 can weld or heat-fuse to seal the fluid channel connected with the reaction cavity in the POCT reagent box, it is convenient to form mutually independent and sealed reaction cavities. The pressing and sealing device for the POCT reagent box provided in the embodiment can drive the sealing part 300 and the pressing part 200 to move to the position of the reaction cavity of the POCT reagent box in turn when the transmission assembly drives the moving support 107 to move in the first direction or the second direction, so that the sealing part 300 can weld or heat-fuse to seal the fluid channel connected with the reaction cavity in the POCT reagent box, forming mutually independent and sealed reaction cavities. When the transmission assembly drives the moving support 107 to move in the opposite direction, the pressing part 200 moves to the reaction cavity of the POCT reagent box and applies horizontal pressure, so that the reaction cavity of the POCT reagent box is tightly attached to the heat source, avoiding the situation that the reaction cavity is unevenly heated due to the difference in surface flatness and insufficient extrusion force. By improving the sealing property of the PCR reaction cavity and the close contact between the PCR reaction cavity and the heating surface, the efficiency of the PCR reaction is improved.

[0097] Preferably, the fixed clamping part further comprises a mounting plate 101 connected with the clamping part 100, so that the clamping part 100 is more stable in structure. In the embodiment, the mounting plate 101 is a plate material with geometric shape and strength, which can bear various structural components on the compression sealing device for the POCT kit. The clamping part 100 is fixedly installed on the left and right sides of the mounting plate 101 by screws, and the clamping part 100 has a kit receiving position for accommodating the POCT kit, which is configured in a clamping groove type to facilitate loading of the POCT kit. The sealing part 300 and the compression part 200 are both located on the first side of the POCT kit loaded in the kit receiving position, and the sealing part 300 and the compression part 200 are arranged in parallel and spaced apart in up and down directions, and the heat source is located on the second side of the POCT kit. Specifically, the sealing part 300 and the compression part 200 are located on the left side of the POCT kit, and the heat source is located on the right side of the POCT kit. When the moving bracket 107 moves relative to the mounting plate 101 in a vertically upward direction, i.e. the first direction is the vertically upward direction, the sealing part 300 moves to the right to the reaction chamber position of the POCT kit to seal the reaction chamber of the POCT kit, and the compression part 200 moves to the left away from the reaction chamber position of the POCT kit; when the moving bracket 107 moves relative to the mounting plate 101 in a vertically downward direction, i.e. the second direction is the vertically downward direction, the compression part 200 moves to the right to the reaction chamber position of the POCT kit, and the sealing part 300 moves to the left away from the reaction chamber position of the POCT kit. In other embodiments of the present application, the first direction is the vertically downward direction, and the second direction is the vertically upward direction.

[0098] Please refer to FIG. 3, which is a side view of the compression sealing device for the POCT kit according to the present application. Optionally, the mounting plate 101 is further provided with a guide rail and a guide rail sliding block 1012, the guide rail is in sliding connection with the guide rail sliding block 1012, and the guide rail sliding block 1012 is connected with the moving bracket 107. When the transmission assembly drives the moving bracket 107 to move, the moving bracket 107 moves along the length direction of the guide rail.

[0099] As shown in FIG. 3 and FIG. 4, the fixed clamping part further comprises a fixed platform 102, a first guide rod 103 and a second guide rod 104, the fixed platform 102 is above the moving bracket 107, the fixed platform 102 is connected with the clamping part 100, the first guide rod 103 and the second guide rod 104 both penetrate through the fixed platform 102 and are movably connected with the fixed platform 102, one end of the first guide rod 103 close to the reagent box receiving position is connected with the sealing part 300, one end of the second guide rod 104 close to the reagent box receiving position is connected with the pressing part 200, when the moving bracket 107 moves along the first direction, the sealing part 300 can move along the length direction of the first guide rod 103 to approach the POCT reagent box, the pressing part 200 can move along the second guide rod 104 to move away from the POCT reagent box, when the moving bracket 107 moves along the second direction, the sealing part 300 can move along the first guide rod 103 to move away from the POCT reagent box, the pressing part 200 can move along the second guide rod 104 to approach the POCT reagent box. In this embodiment, the fixed platform 102, the first guide rod 103 and the second guide rod 104 form a guide assembly of the fixed clamping part, the fixed platform 102 is transversely and fixedly installed on the inner side of the clamping part 100, the first guide rod 103 and the second guide rod 104 are arranged in parallel above and below the fixed platform 102, the first guide rod 103 and the second guide rod 104 both penetrate through the fixed platform 102 and are slidably connected with the fixed platform 102.

[0100] Further, one end of the first guide rod 103 away from the reagent box receiving position is provided with a limiting ring 105, and one end of the second guide rod 104 away from the reagent box receiving position is also provided with a limiting ring 105. During the horizontal rotation of the pressing part 200 and the sealing part 300, the limiting ring 105 can prevent the first guide rod 103 and the second guide rod 104 from falling off from the fixed platform 102. In this embodiment, the limiting ring 105 is clamped near the end of the first guide rod 103 away from the reagent box receiving position, and the limiting ring 105 is clamped near the end of the second guide rod 104 away from the reagent box receiving position.

[0101] Further, the mounting plate 101 is provided with a photoelectric sensor 106, preferably, the photoelectric sensor 106 is arranged on the top of the mounting plate 101, the photoelectric sensor 106 can monitor the moving height of the moving bracket 107, so as to control the distance of the horizontal rotation of the pressing part 200 and the sealing part 300 along the guide assembly. In this embodiment, the photoelectric sensor 106 is arranged on the top of the mounting plate 101, and the total stroke of the sealing part 300 and the pressing part 200 in the horizontal direction is 6mm.

[0102] Please continue to refer to Figure 2, and refer to Figure 5 and Figure 6, Figure 5 is the structure schematic diagram of the fixed clamping part and the transmission assembly in the compression sealing device for the POCT kit provided by the application, and Figure 6 is the structure schematic diagram of the compression sealing device before working provided by the application. The fixed clamping part further comprises a first rotating rod 108, a second rotating rod 109, a first connecting rod 110 and a second connecting rod 120. The mounting plate 101 is fixed on the transmission assembly, and a stand 1011 is arranged on the mounting plate 101. The stand 1011 is distributed on both sides of the moving bracket 107. The first connecting rod 110 penetrates the first connecting hole on the stand 1011 on both sides of the moving bracket 107. The second connecting rod 120 penetrates the second connecting hole on the stand 1011 on both sides of the moving bracket 107. The moving bracket 107 is provided with a compression part movable hole 1071 and a sealing part movable hole 1072. The second rotating rod 109 penetrates the compression part movable hole 1071, and the first rotating rod 108 penetrates the sealing part movable hole 1072. The structures on both sides of the moving bracket 107 are symmetrical. On the first side of the moving bracket 107, the first connecting rod 110 is movably connected with the first rotating rod 108, the first rotating rod 108 is movably connected with the sealing part 300, the second connecting rod 120 is movably connected with the second rotating rod 109, and the second rotating rod 109 is movably connected with the compression part 200. When the transmission assembly drives the moving bracket 107 to move, the first rotating rod 108 drives the first connecting rod 110 to move, the sealing part 300 moves away from or approaches the POCT kit, the second rotating rod 109 drives the second connecting rod 120 to move, and the compression part 200 approaches or moves away from the POCT kit. The movable connection mode can be hinged connection, and the movable connection mode can also be rotary connection or other connection modes.

[0103] Preferably, the first connecting rod 110 is hinged with the first rotating rod 108 to form a first connecting part 1101, the first rotating rod 108 is hinged with the sealing part 300 to form a second connecting part 1102, and the included angle range between the first connecting part 1101 and the second connecting part 1102 in the clockwise direction is [85°-180°). The angle range can enable the sealing part 300 to apply a large compression force to the POCT kit, so as to realize the sealing function of the POCT kit reaction cavity when the sealing part 300 moves to the POCT kit. The second connecting rod 120 is hinged with the second rotating rod 109 to form a third connecting part 1201, and the second connecting rod 120 is hinged with the compression part 200 to form a fourth connecting part 1202. The included angle range between the third connecting part 1201 and the fourth connecting part 1202 in the counterclockwise direction is [85°-180°). The angle range can enable the compression part 200 to apply a large compression force to the POCT kit, so as to realize the full compression function of the reaction cavity when the compression part 200 moves to the POCT kit reaction cavity, and avoid the situation of uneven heating.

[0104] In the embodiment, the number of the first connecting rods 110 and the second connecting rods 120 is both 2, the first connecting rods 110 are rotatably installed on both sides of the first rotating rod 108, and the second connecting rods 120 are rotatably installed on both sides of the second rotating rod 109. Specifically, the first connecting holes and the second connecting holes are arranged in parallel in the vertical direction on the upright column 1011. One of the first connecting rods 110 and one of the second connecting rods 120 are respectively inserted into the first connecting hole and the second connecting hole on the upright column 1011 of the mounting plate 101 and are rotatably installed thereon, and are respectively hinged to the first rotating rod 108 and the second rotating rod 109. The other first connecting rod 110 and the other second connecting rod 120 are respectively rotatably installed on the sealing part 300 and the pressing part 200, and are respectively hinged to the first rotating rod 108 and the second rotating rod 109, so that the first connecting rod 110 is hinged to the first rotating rod 108 to form a first connecting part 1101, the first rotating rod 108 is hinged to the sealing part 300 to form a second connecting part 1102, the second connecting rod 120 is hinged to the second rotating rod 109 to form a third connecting part 1201, and the second connecting rod 120 is hinged to the pressing part 200 to form a fourth connecting part 1202. In order to obtain a larger pressing force, the included angle between the first connecting part 1101 and the second connecting part 1102 in the clockwise direction is in the range of [85°-180°), and the included angle between the third connecting part 1201 and the fourth connecting part 1202 in the counterclockwise direction is in the range of [85°-180°). For example, when the power source of the transmission assembly, hereinafter the driving motor of the transmission assembly is marked as the first driving motor 401, the output thrust of the first driving motor 401 is 75N, and the included angle between the third connecting part 1201 and the fourth connecting part 1202 in the counterclockwise direction is 179°, at this time, the maximum extrusion force of the pressing part 200 is 600N. Specifically, when the first driving motor 401 rotates in the forward direction, the moving bracket 107 moves upward, the second rotating rod 109 drives the second connecting rod 120 to move upward, as the included angle between the third connecting part 1201 and the fourth connecting part 1202 in the counterclockwise direction gradually decreases, the pressing part 200 moves horizontally to the left along the guide assembly, as the included angle between the first connecting part 1101 and the second connecting part 1102 in the clockwise direction gradually increases to the optimal angle, i.e., the included angle between the first connecting part 1101 and the second connecting part 1102 in the clockwise direction approaches 180°, and then the sealing part 300 moves horizontally to the right along the guide assembly, when the first driving motor 401 reverses rotation, the above movement is just the opposite.

[0105] Please continue to refer to Figure 5, the power source of the transmission assembly in the compression sealing device is the first drive motor 401, the assembly also includes a base 402, a nut 403, a mounting seat 404 and a lead screw 405. Specifically, the base 402 is located above the first drive motor 401 and connected with the motor, the base 402 is fixedly connected with the mounting plate 101, the lead screw 405 is in transmission connection with the first drive motor 401, the nut 403 is in transmission connection with the lead screw 405, the mounting seat 404 is located above the nut 403, the mounting seat 404 is connected with the moving bracket 107, when the first drive motor 401 is in transmission with the lead screw 405, the nut 403 drives the mounting seat 404 and the moving bracket 107 to move along the length of the lead screw 405 to the first direction or the second direction. In this embodiment, the first drive motor 401 is fixedly installed at the bottom end of the base 402, the motor is opposite to the lower side of the fixed table 102, the base 402 is fixed on the mounting plate 101 by screws, the base 402 can be used to fix the motor, the nut 403 is installed on the lead screw 405 of the motor, the upper end surface of the nut 403 is fixedly installed with the moving bracket 107 through the mounting seat 404, the moving bracket 107 and the mounting seat 404 can move up and down along the lead screw 405 on the motor with the nut 403, in addition, when the mounting plate is provided with a guide rail and a guide rail sliding block 1012, the length direction of the guide rail is consistent with the length direction of the lead screw 405.

[0106] Further, the sealing part 300 is provided with a hot melt bonding part 301 to facilitate sealing of the reaction cavity, one side of the sealing part 300 provided with the hot melt bonding part 301 is close to the reaction cavity of the POCT reagent box, and the sealing part 300 is hinged with the moving bracket 107. In this embodiment, the compression part 200 and the sealing part 300 are arranged in parallel, the sealing part 300 includes the hot melt bonding part 301, which is a metal device with certain heat conduction performance, the hot melt bonding part 301 can be hot melt or welded to destructively block the fluid channel connected with the reaction cavity in the POCT reagent box, so as to realize the sealing effect of the reaction cavity.

[0107] Please refer to Figure 7, the compression part 200 includes a pressure head seat 2019 and a pressure head 2029 connected with each other, the pressure head seat 2019 is hinged with the moving bracket 107, the pressure head seat 2019 is connected with the second guide rod 104, the pressure head 2029 is provided with a boss 2021 on the side close to the reagent box receiving position, when the compression part 200 abuts against the first side of the reaction cavity of the POCT reagent box, the boss 2021 is tightly combined with the reaction cavity of the POCT reagent box. Since the compression part 200 can extrude the POCT reagent box, the reaction cavity in the POCT reagent box can be in full contact with the heating element, so as to improve the efficiency of the amplification reaction. In order to reduce the uneven heating caused by the difference in the surface flatness of the reaction cavity in the POCT reagent box, the pressure head 2029 is detachably installed on the pressure head seat 2019 by screws, so that the reaction cavity can be in full contact with the heating element.

[0108] Further, the center of the boss 2021 is provided with a fiber hole 2022. That is to say, the surface of the pressure head 2029 is provided with the boss 2021 which is independent of each other and has the fiber hole 2022 for inserting the optical fiber, and the fiber hole for detecting the optical fiber forms the optical fiber detection hole.

[0109] The working principle of the present application is described as follows:

[0110] First step, set the compression and sealing device to zero position;

[0111] Before installing the POCT kit, the mobile support 107 is controlled to be at a preset height by the photoelectric sensor 106, at this time, the compression part 200 and the sealing part 300 are basically flush, at this time, the compression and sealing device is in zero position, as shown in FIG. 6.

[0112] Second step, seal the fluid channel of the reaction chamber of the POCT kit;

[0113] The first driving motor 401 works, when rotating in the forward direction, the mobile support 107 follows the nut 403 to move upward along the lead screw 405, since the first rotating rod 108 is hingedly connected with the first connecting rod 110, and the second rotating rod 109 is hingedly connected with the second connecting rod 120, at this time, the first connecting part 1101 forms an angle close to 180° with the second connecting part 1102 in the clockwise direction, similarly, the third connecting part 1201 forms an angle gradually decreasing to the minimum value 85° with the fourth connecting part 1202 in the counterclockwise direction, under the traction of the second rotating rod 109, the compression part 200 moves horizontally to the left along the second guide rod 104 in the guide assembly, at this time, the sealing part 300 moves horizontally to the right along the first guide rod 103 in the guide assembly under the traction of the first rotating rod 108, as shown in FIG. 8, the heated hot melt joint part 301 contacts the POCT kit, realizing the sealing effect on the fluid channel of the PCR reaction chamber.

[0114] Third step, realize the close contact between the reaction chamber of the POCT kit and the heating surface of the heating element;

[0115] The first driving motor 401 rotates reversely, the moving support 107 moves downwards along the screw nut 403 following the screw rod 405, at this time, the first connecting part 1101 gradually reduces the included angle formed with the second connecting part 1102 in the clockwise direction to 85 degrees, similarly, the third connecting part 1201 gradually increases the angle formed with the fourth connecting part 1202 in the counterclockwise direction until close to the maximum value 180 degrees, under the traction of the second rotating rod 109, as shown in Figure 9, at this time, the sealing part 300 moves horizontally to the left along the first guide rod 103 in the guide assembly under the traction of the first rotating rod 108, the compression part 200 moves horizontally to the right along the second guide rod 104 in the guide assembly, the compression part 200 is in close contact with the reaction cavity in the POCT reagent box, and the close contact between the reaction cavity in the POCT reagent box and the heating surface of the heating element is realized.

[0116] The compression and sealing device for the POCT reagent box provided in the embodiment is integrated with sealing and compression through the cooperative action of the moving support, the first rotating rod, the second rotating rod, the first connecting rod, the second connecting rod, the compression part and the sealing part, the device not only can realize the sealing function of the reaction cavity of the POCT reagent box, but also can realize the sufficient compression function of the reaction cavity, and the uneven heating condition is avoided. In addition, through the first connecting rod and the second connecting rod capable of adjusting the included angle, the compression part and the sealing part can obtain a large enough extrusion force in the horizontal direction, and the bosses independent of each other are designed on the compression head, the area of the compression head in contact with the reaction cavity is increased, and thus the uneven heating condition of the reaction cavity caused by the difference in the flatness of the reaction cavity surface and the insufficient extrusion force is avoided, the sealing property of the PCR reaction cavity is improved and the close contact between the PCR reaction cavity and the heating surface is strengthened by applying the device to the molecular diagnostic equipment, and thus the reaction efficiency of the reaction cavity of the POCT reagent box is improved, and the work efficiency is greatly improved.

[0117] In addition, the compression and sealing device for the POCT reagent box provided in the embodiment is integrated with sealing and compression through the first rotating rod, the second rotating rod, the first connecting rod, the second connecting rod, the compression part and the sealing part, the device has the double functions of sealing and compression, the structure is simple, the sealing and compression work of the POCT reagent box is convenient for the staff, the operation is simple, time and labor are saved, the compatibility is good, the manufacturing cost is low, the production cycle is short, the safety factor is high, and the device has a broad application prospect.

[0118] Embodiment two

[0119] The application further provides a method for sealing and compression by using the compression and sealing device for the POCT reagent box in the embodiment one.

[0120] The compression sealing device for the POCT kit comprises a fixed clamping part, a compression part 200, a sealing part 300 and a transmission assembly. Specifically, the fixed clamping part comprises a clamping part 100 capable of clamping the POCT kit and a moving support 107, and the transmission assembly is capable of driving the moving support 107 to move in a first direction or a second direction, so that the moving support 107 drives the compression part 200 and the sealing part 300 to alternately contact or move away from the POCT kit, the sealing part 300 seals the reaction chamber of the POCT kit when it contacts the POCT kit, and the compression part 200 makes the reaction chamber of the POCT kit tightly adhere to the heat source when it contacts the POCT kit. For example, when the transmission assembly drives the moving support 107 to move in the first direction, the moving support 107 drives the compression part 200 to move away from the POCT kit, while the moving support 107 drives the sealing part 300 to approach and contact the POCT kit, and when the transmission assembly drives the moving support 107 to move in the second direction, the moving support 107 drives the compression part 200 to approach and contact the POCT kit, while the moving support 107 drives the sealing part 300 to move away from the POCT kit. Since the sealing part 300 and the compression part 200 alternately approach or move away from the POCT kit in time sequence, the sealing part 300 seals the reaction chamber of the POCT kit when it contacts the POCT kit, avoiding the influence of the compression part 200, and the compression part 200 makes the reaction chamber of the POCT kit tightly adhere to the heat source when it contacts the POCT kit, also avoiding the influence of the sealing part 300.

[0121] The sealing and compression method comprises the following steps:

[0122] S10, control the moving support 107 to be at a preset height, so that the compression part 200 and the sealing part 300 are flush;

[0123] S20, install the POCT kit at the clamping part 100, and drive the moving support 107 to move in the first direction by the transmission assembly, so that the moving support 107 drives the sealing part 300 to approach and contact the POCT kit, and at the same time, the moving support 107 drives the compression part 200 to move away from the POCT kit;

[0124] S30, control the sealing part 300 to abut against one side of the POCT kit, and realize the sealing of the reaction chamber of the POCT kit;

[0125] S40, drive the moving support 107 to move in the second direction by the transmission assembly, so that the moving support 107 drives the compression part 200 to approach and compress the reaction chamber of the POCT kit, and at the same time, the moving support 107 drives the sealing part 300 to move away from the POCT kit;

[0126] S50, the pressing part 200 controls the reaction chamber of the POCT kit to be tightly contacted with the heat source on the other side and heat transfer is performed, so that the polymerase chain reaction in the reaction chamber of the POCT kit is completed.

[0127] The method for sealing and pressing provided by the pressing and sealing device for the POCT kit can realize the sealing and sufficient pressing of the reaction chamber of the POCT kit, avoid uneven heating, and enable the pressing part and the sealing part to obtain a large enough extrusion force in the horizontal direction, thereby avoiding the uneven heating of the reaction chamber caused by the difference in the flatness of the reaction chamber surface and the insufficient extrusion force, improving the sealing of the PCR reaction chamber and the close contact between the PCR reaction chamber and the heating surface, and further improving the reaction efficiency of the reaction chamber of the POCT kit, greatly improving the work efficiency, and being simple in operation, time-saving and labor-saving, low in use cost, short in production cycle and high in safety factor.

[0128] Embodiment three

[0129] The amplification device also comprises all the functional components of the pressing and sealing device for the POCT kit in the embodiment one.

[0130] The pressing and sealing device for the POCT kit in the amplification device also comprises any other components mentioned in the embodiment one, which will not be described here. The amplification device comprises the pressing and sealing device for the POCT kit, integrates sealing and pressing, can realize the sealing function of the reaction chamber of the POCT kit, and can realize the sufficient pressing function of the reaction chamber, avoid uneven heating, meet the sealing and uniform heating requirements of the PCR reaction chamber, and greatly improve the work efficiency of the device.

[0131] In one case, the molecular diagnostic device can also comprise a schematic diagram of a sample tube 1000 as shown in FIG. 10, which can contain a sample preservation liquid, so that the collected sample liquid can be long-term preserved. The sample can be a nasal swab, a throat swab, or other types of swabs. In other cases, the sample can be saliva, blood, tissue fluid, or the like. Of course, the sample tube 1000 can also contain a lysis liquid, so that the sample can be fully lysed by manual action and directly added to the POCT kit. Of course, in a special case, the sample tube 1000 can not be contained and the sample can be directly added to the POCT kit 10.

[0132] The POCT kit 10 provided by the present application has the structure as shown in FIG. 11. The POCT kit 10 can be adapted to the molecular diagnostic equipment of the present embodiment, which is also called small molecular diagnostic POCT device. The POCT kit 10 contains different chambers. In actual operation, in order to smoothly drive the liquid to be accurately transferred between different chambers without the risk of leakage, the POCT kit 10 is vertically inserted into the molecular diagnostic equipment, so that different components in the molecular diagnostic equipment can act on the POCT kit 10 to make the liquid therein flow in different chambers according to the preset path. A sample inlet is arranged in the upper area of the POCT kit 10 in the vertical direction. In order to ensure the stability and sealing operation of the reagents inside the POCT kit 10, the sample inlet is detachably connected with a kit cover 1010. The two can be connected in a detachable manner such as buckling or threading. In order to meet the needs of convenience, low cost and other needs of production and processing, the POCT kit 10 is provided with a buffer chamber 12 and a system chamber 13. The two are connected through a transfer flow channel. At least one position of the transfer flow channel is provided with a flow control valve 123, so as to change the opening degree of the flow control valve 123 to change the connection relationship between the buffer chamber 12 and the system chamber 13. In order to ensure that the buffer chamber 12 and the system chamber 13 have a large volume and can basically transfer the liquid therein without residue, the two are formed in a substantially same structure, both having an upper part of the same cross-section cylindrical container part and a bottom part of the tapered flow guide part which is gradually reduced from top to bottom. One end of the transfer flow channel is connected to the flow guide part at the bottom of the buffer chamber 12, and the other end is connected to the flow guide part at the bottom of the system chamber 13. The flow guide part at the bottom of the system chamber 13 is also connected with a distribution flow channel. The connection port of the distribution flow channel corresponding to the system chamber 13 is arranged in a spaced manner with the connection port of the transfer flow channel corresponding to the system chamber 13.

[0133] A plurality of amplification cavities 14 are arranged in the middle region of the POCT kit 10, and the specific number can be set according to the requirements. In order to ensure the balance of flow resistance, the amplification cavities 14 are optimally arranged in an even number. The amplification cavities 14 are optimally arranged in a shape with varying cross-sectional areas, including droplet type or similar droplet type, egg type, ellipsoid, similar cone or other revolution body, so that the liquid filling process can be fully developed, the turbulence is smaller, the probability of air gap is smaller, and the filling degree in the amplification cavity 14 is higher. The filling degree can reach 95%-100%, the subsequent amplification is more reliable, and the detection accuracy is higher. A liquid distribution driving part 15 is arranged at the lower part of the POCT kit 10. In order to ensure that the liquid distribution driving part 15 can uniformly drive the liquid filling in the plurality of amplification cavities 14, the liquid distribution driving part 15 can include a plurality of liquid distribution driving units corresponding to the number of amplification cavities 14. In the embodiment, the amplification cavities 14 and the liquid distribution driving units in the liquid distribution driving part 15 are arranged as hollow cavity structures formed on the consumable substrate of the POCT kit 10, and then the two opposite surfaces in the thickness direction are attached with the deformable and recoverable film. The deformable and recoverable film can be made of propylene, polyethylene, polystyrene, cyclic olefin copolymer (COC), polyester film, polyacetate, etc. It can also be made of cortical material and rubber material. The deformable and recoverable film can also be a metal film that meets the requirements. Of course, the deformable and recoverable film can also be a composite film composed of multiple materials. The film has an optimal light transmittance of 80%-95%. In order to balance the requirements of film strength, light transmittance and thermal conductivity, the film with an optimal thickness of 0.02mm-0.12mm is selected. In the closed POCT kit 10, one side of the amplification cavity 14 can directly contact and transfer heat with the thermal cycle part through the film. Therefore, the film with the optimal thickness can ensure the highest heat transfer efficiency. The liquid distribution driving cavity and the attached film form a liquid distribution driving unit, and the clamping device can press at least part of the film corresponding to the liquid distribution driving unit. The film at the clamping position is deformed. After the clamping device is released, the deformed film forms a suction effect during the deformation recovery process, so that the liquid to be distributed in the system cavity 13 is driven to fill the amplification cavity 14. Therefore, the deformable and recoverable film itself has sufficient strength and deformation recovery ability, so that the design of the entire POCT kit 10 is simpler, and the problem of affecting the detection quality due to the influence of the light transmittance of the thick film is avoided. In the embodiment, a plurality of hollow combination auxiliary parts 1610 are arranged between the amplification cavities 14 and the liquid distribution driving part 15.

[0134] Figure 12 is a schematic diagram of the structure of the molecular diagnostic equipment of the present application, and Figure 13 is a schematic diagram of the structure of the molecular diagnostic equipment receiving the POCT kit 10; the molecular diagnostic equipment comprises a device body 2, which is provided with two POCT kit receiving portions, here marked as a first kit receiving portion 2001 and a second kit receiving portion 2002, and the structures of the two receiving portions are consistent, both of which are provided with the clamping portion in Example One and are arranged with kit receiving positions to receive the POCT kit 10. A control portion 2020 is arranged at the front of the molecular diagnostic equipment, the control portion 2020 contains an interface capable of interacting with the user, so that the molecular diagnostic equipment can be independently operated by the user. A device air inlet 2030 is arranged below the control portion 2020. In this embodiment, the POCT kit 10 is in a closed state, so the device air inlet 2030 can provide a flowing heat dissipation air source for each module in the molecular diagnostic equipment. Here, the first kit receiving portion 2001 and the second kit receiving portion 2002 both contain a receiving cover 30 matched therewith, the receiving cover 30 can be connected with the device body 2 by buckling, and the receiving cover 30 is further provided with a cap accommodating portion 32 and an air source interface 31. Here, the air source driving member is in communication with the air source interface 31 through an air source connecting pipe. The cap accommodating portion 32 is used to accommodate the cap 1010 on the POCT kit 10 when the receiving cover 30 is buckled to the device body 2. The air source interface 31 can be in fluid communication with the air source driving member 25. Here, the side of the air source interface 31 matched with the POCT kit 10 is further provided with a rubber pad. When the receiving cover 30 is buckled to the device body 2 through the buckle 3010, the air source interface 31 can seal and press the air inlet 111 of the POCT kit 10 through the rubber pad. The air inlet 111 is in communication with the buffer cavity 12, so that the air source driving member 25 can be in fluid communication with the POCT kit 10. The system cavity 13 is in communication with the external environment through the air return port 112. The system cavity 13 can inhale or exhaust air through the air return port 112. When the liquid is transferred in the system cavity 13, the airflow balance in the system cavity 13 is maintained. In this embodiment, the first kit receiving portion 2001 and the second kit receiving portion 2002 can be independently operated. Two POCT kits 10 can be received at the same time to perform efficient simultaneous detection. Alternatively, two POCT kits 10 can be received respectively. While one POCT kit 10 is performing detection, another POCT kit 10 can be added to the device body 2. For example, while the first kit receiving portion 2001 is performing detection, the second kit receiving portion 2002 can receive a POCT kit 10 as it arrives, so as to adapt to the scene requirement of detection as it arrives.

[0135] Fig. 14 is a state diagram of the present application receiving a single POCT reagent kit 10 in the kit receiving part, and Fig. 15 is a state diagram of the present application receiving a single POCT reagent kit 10 from another perspective; here the first kit receiving part 2001 and the second kit receiving part 2002 are both configured with a kit receiving position 201 that can independently receive a POCT reagent kit 10, and after the POCT reagent kit 10 is correctly placed in one of the first kit receiving part 2001 and the second kit receiving part 2002, the POCT reagent kit 10 can be clamped in the kit receiving position 201; here in order to ensure that the POCT reagent kit 10 can be reliably clamped, the kit receiving position 201 includes two opposing vertical clamping grooves, and in this embodiment the kit receiving position 201 is configured with a thermal cycling part 23 on one side along the thickness direction of the POCT reagent kit 10, and a detection and collection unit 26 and a hot melt bonding part 301 on the other side; here the hot melt bonding part 301 is a functional component included in the sealing part 300 in Embodiment 1; the thermal cycling part 23 includes a fan 231, a fin heat dissipation unit 232, a heating part 233, and a heat pipe heat exchanger 234; the fan 231 is matched with the fin heat dissipation unit 232; the heating part 233 is matched with the consumable receiving position 201, and when the consumable receiving position 201 receives the POCT reagent kit 10, the heating surface of the heating part 233 can contact the amplification cavity 14 of the POCT reagent kit 10, achieving efficient heat transfer and heating of the liquid to be amplified in the amplification cavity 14, and more specifically, the heating surface of the heating part 233 can directly contact and act on the corresponding film of the amplification cavity 14;The heat pipe heat exchanger 234 is arranged between the fin heat dissipation unit 232 and the heating part 233, the cold end of the heat pipe heat exchanger 234 is arranged in the fin heat dissipation unit 232, and the hot end is embedded and connected in the heating part 233. The detection and collection unit 26 and the hot melt bonding part 301 are arranged on the other side of the POCT reagent box 10 in the thickness direction. The detection and collection unit 26 can be included in the pressing part 200 in Embodiment One, so that the first driving motor 401 can be used to drive the detection and collection unit 26 and the hot melt bonding part 301 alternately in time sequence, and the hot melt bonding part 301 and the detection and collection unit 26 are movably connected to the equipment body 2 through the corresponding driving part. The first driving motor 401 can drive the alternate transmission mechanism 22 to drive the hot melt bonding part 301 and the detection and collection unit 26 away from the POCT reagent box 10, or drive one of the hot melt bonding part 301 and the detection and collection unit 26 to approach and act on the POCT reagent box 10, as shown in Figures 5 and 6. The alternate transmission mechanism 22 is arranged in the fixed clamping part, including the first rotating rod 108, the second rotating rod 109, the first connecting rod 110 and the second connecting rod 120, the moving bracket 107, the vertical column 1011, the mounting plate 11, the first connecting part 1101, the second connecting part 1102, the third connecting part 1201 and the fourth connecting part 1202. The second rotating rod 109 is connected to the detection and collection unit 26 through the fourth connecting part 1202. One side of the fourth connecting part 1202 is hinged to the second rotating rod 109, and the other side is hinged to the detection and collection unit 26. When the first driving motor 401 drives the moving bracket 107 to move upward along the vertical direction, as shown in Figure 8, the first rotating rod 108 and the second rotating rod 109 move upward with the moving bracket 107. The first rotating rod 108 moves horizontally along the pressing part movable hole 1071, so that the third connecting part 1201 and the fourth connecting part 1202 form an upward bending angle, thereby driving the detection and collection unit 26 to retreat away from the reagent box receiving position 201 along the horizontal direction. At the same time, the second rotating rod 109 moves horizontally along the sealing part movable hole 1072, so that the first connecting part 1101 and the second connecting part 1102 form a horizontal straight line structure, thereby driving the hot melt bonding part 301 to stretch out along the horizontal direction towards the reagent box receiving position 201.When the first driving motor 401 drives the moving bracket 107 to move downward along the vertical direction, as shown in FIG. 6, the first rotating rod 108 and the second rotating rod 109 move downward along with the moving bracket 107, the first rotating rod 108 moves horizontally along the pressing part movable hole 1071, so that the third connecting part 1201 and the fourth connecting part 1202 form an upwardly bent folding angle, and the detection and collection unit 26 is driven to extend horizontally toward the consumable receiving position 201, and at the same time, the second rotating rod 109 moves horizontally along the sealing part movable hole 1072, so that the first connecting part 1101 and the second connecting part 1102 form a downwardly bent folding angle, and the hot melt combination part 301 is driven to retreat horizontally away from the reagent box receiving position 201, at this time, the detection and collection unit 26 and the hot melt combination part 301 are both spaced from the reagent box receiving position 201, as the first driving motor 401 continues to drive the moving bracket 107 to move downward along the vertical direction, as shown in FIG. 9, a horizontal straight line structure is formed between the third connecting part 1201 and the fourth connecting part 1202, and the detection and collection unit 26 is driven to be pressed in the POCT reagent box 10 in the reagent box receiving position 201, and at the same time, the second rotating rod 109 moves horizontally along the sealing part movable hole 1072, so that the first connecting part 1101 and the second connecting part 1102 form a downwardly bent folding angle, and the hot melt combination part 301 is driven to retreat horizontally away from the reagent box receiving position 201, the process is similar to the driving process of the pressing part 200 and the sealing part 300 in the pressing and sealing device, but the timing of the operation of the molecular diagnostic equipment is described in detail.

[0136] In the actual configuration, the detection and collection unit 26 and the hot melt combination part 301 are configured closer to the equipment air inlet 2030 at the front end of the molecular diagnostic equipment, and the thermal cycling part 23 is configured at the rear end of the molecular diagnostic equipment close to the equipment air outlet, so that the air input into the molecular diagnostic equipment through the equipment air inlet 2030 can flow through the second driving motor 241 and the hot melt combination part 301 in time, and the waste heat in the molecular diagnostic equipment can be effectively taken out of the molecular diagnostic equipment through the action of the fan 231.

[0137] FIG. 16 is a state diagram of two reagent box receiving positions 201 receiving two POCT reagent boxes 10; in this configuration, the first reagent box receiving part 2001 and the second reagent box receiving part 2002 are both configured with POCT reagent boxes 10, in this embodiment, two POCT reagent boxes 10 can be clamped in the corresponding reagent box receiving position 201, the first reagent box receiving part 2001 and the second reagent box receiving part 2002 both contain independent thermal cycling parts 23, detection and collection units 26 and hot melt combination parts 301, so that the POCT reagent boxes 10 in the two reagent box receiving positions 201 can be detected simultaneously or separately.

[0138] Figure 17 is a side view of the POCT kit 10 of the present application, and Figure 18 is a side view of the POCT kit 10 with the valve control part 21 and the clamping device 24 being synchronously driven. In this embodiment, a transfer flow channel is arranged near the top of the POCT kit 10, which can communicate with the buffer chamber 12 and the system chamber 13, and a flow control valve 123 is arranged at the transfer flow channel, which can open or close the transfer flow channel. A liquid distribution driving part 15 is arranged at the bottom of the POCT kit 10. The flow control valve 123 is a magnetic valve. When the valve control part 21 is not close to the flow control valve 123, the flow control valve 123 is in a normally closed state, which closes the transfer flow channel between the buffer chamber 12 and the system chamber 13. When the magnet 211 on the valve control part 21 is close to the flow control valve 123, the flow control valve 123 can be unsealed from the transfer flow channel between the buffer chamber 12 and the system chamber 13 under the magnetic attraction of the magnet 211, so that the transfer flow channel is opened. Thus, the gas source driving part 25 can be connected to the buffer chamber 12 and change the air pressure in the buffer chamber 12. When the air pressure in the buffer chamber 12 increases, the liquid can flow from the buffer chamber 12 to the system chamber 13 through the opened transfer flow channel. When the air pressure in the buffer chamber 12 decreases, the liquid can flow from the system chamber 13 to the buffer chamber 12 through the opened transfer flow channel. The clamping device 24 can exert a clamping force on the liquid distribution driving part 15, so that the pressure in the amplification chamber 14, which is in fluid communication with the liquid distribution driving part 15, increases, which can limit the liquid from flowing from the system chamber 13 to the amplification chamber 14, and also ensures the reliability of the liquid flow driven by the gas source driving part 25, effectively reducing the risk of contamination.

[0139] Fig. 19 is a schematic diagram of the valve control part 21 and the clamping device 24 sharing the second driving motor 241, and Fig. 20 is a schematic diagram of the valve control part 21 and the clamping device 24 acting on the POCT reagent kit 10. During the driving of the gas source driving member 25 to transfer the liquid between the buffer cavity 12 and the system cavity 13, it is necessary to limit the flow of the liquid from the system cavity 13 to the amplification cavity 14. Since the amplification cavity 14 is in fluid communication with the liquid separation driving part 15, the liquid separation driving part 15 can be adjusted to make the amplification cavity 14 in a positive pressure state, thereby forming a resistance to limit the flow of the liquid between the amplification cavity 14 and the system cavity 13. In order to realize the reliability of the system operation, the valve control part 21 and the clamping device 24 share the second driving motor 241 in the embodiment, the integration of the whole system is higher, the control difficulty is low, and the communication relationship between the buffer cavity 12 and the system cavity 13, and the amplification cavity 14 and the system cavity 13 can be synchronously adjusted. In order to ensure that the clamping device 24 can be driven with high precision, the output of the second driving motor 241 is connected to the transmission screw rod 242 through a shaft coupling. The transmission screw rod 242 is a double-thread transmission screw rod including a right-handed thread segment and a left-handed thread segment. In order to ensure that the clamping device 24 can more reliably apply a pressing force to the liquid separation driving part 15, the clamping device 24 includes a pair of clamping units arranged oppositely and threadedly fitted to different direction thread segments of the transmission screw rod 242. When the second driving motor 241 rotates, the pair of clamping units can relatively approach, thereby pressing the liquid separation driving part 15 from both sides in the thickness direction of the POCT reagent kit 10. Compared with the single-side pressing scheme, the two-side pressing makes the film of the liquid separation driving part 15 have a smaller deformation amount when being pressed, and the pressure adjustment range is larger. In order to ensure that each amplification cavity 14 has substantially the same driving pressure, the liquid separation driving part 15 includes a plurality of liquid separation driving units, and correspondingly, the clamping unit includes a connecting part and a chuck corresponding to the liquid separation driving units one by one. One end of the connecting part is threadedly connected to the transmission screw rod 242, the other end is connected to the chuck, and the chuck is convex to the connecting part. The valve control part 21 is connected to and follows the corresponding side clamping unit through a transmission block. When the second driving motor 241 drives the clamping device 24 to clamp the liquid separation driving part 15, the valve control part 21 synchronously approaches the flow control valve 123, so that the flow control valve 123 is opened, and the communication between the buffer cavity 12 and the system cavity 13 and the closure between the amplification cavity 14 and the system cavity 13 are synchronously performed. When the second driving motor 241 drives the clamping device 24 to release the liquid separation driving part 15, the valve control part 21 synchronously moves away from the flow control valve 123, so that the flow control valve 123 is closed, and the closure between the buffer cavity 12 and the system cavity 13 and the communication between the amplification cavity 14 and the system cavity 13 are synchronously performed. In order to ensure the smooth and reliable characteristics of the transmission operation, a transmission guide rail 243 parallel to the length direction of the transmission screw rod 242 is further provided. The connecting part is movably connected to the transmission guide rail 243, and the transmission guide rail 243 provides a support force to the connecting part.

[0140] Figure 21 is a schematic diagram of the structure of the air source driving element 25 connected to the buffer cavity 12 in the open state of the flow control valve 123; the flow control valve 123 is in the open state driven by the common second driving motor 241, and the air source driving element 25 can be connected to the buffer cavity 12 of the POCT reagent kit 10 through the air source interface 31 on the buckled receiving cover 30, where the air source driving element 25 can be a plunger pump type driving element, which changes the air pressure state in the buffer cavity 12 through the suction action of the plunger, and then makes the liquid transfer between the buffer cavity 12 and the system cavity 13, at the same time, due to the compression of the clamping device 24 to the distribution driving part 15, the liquid in the system cavity 13 can be limited to enter the amplification cavity 14, of course, the air source driving element 25 can output driving to make the liquid flow several times between the buffer cavity 12 and the system cavity 13 to realize the full mixing of various reagents and samples.

[0141] Figure 22 is a layout structure diagram of the air source driving element 25 and the second driving motor 241; in order to ensure the rationality of the system configuration, a middle substrate 2000 is configured in the molecular diagnostic equipment, the reagent kit receiving position 201 is arranged on the upper part of the middle substrate 2000, and the thermal cycle part 23, the detection and collection unit 26 and the hot melt combination part 301 are distributed on both sides thereof, the lower part of the middle substrate 2000 is arranged with two air source driving elements 25, two second driving motors 241 and two first driving motors 401, so that the centralized arrangement of the motion driving unit can minimize the noise during operation, and also make the maintenance of the molecular diagnostic equipment simpler, in one case, the two air source driving elements 25 are arranged in the middle and the two second driving motors 241 are arranged on both sides of the two air source driving elements 25, so that the risk of resonance during the operation of the whole machine is smaller.

[0142] Figure 23 is a schematic diagram of the state of the air source driving element 25 driving the liquid to flow between the system cavity 13 and the buffer cavity 12 in the open state of the flow control valve; in this embodiment, the reagent in the system cavity 13 is configured in a freeze-dried state, so that the liquid is driven to flow several times between the buffer cavity 12 and the system cavity 13, which can fully dissolve the freeze-dried reagent to form a system liquid with higher sensitivity.

[0143] Figure 24 is a state diagram of the liquid in the system cavity 13, and Figure 25 is a state diagram of the liquid in the system cavity 13 being driven by the liquid distribution drive 15 to the amplification cavity 14; the mixed liquid, i.e. the system liquid, can be driven by the air source drive 25 to be stored in the system cavity 13, at this time the second drive motor 241 can output rotary drive to make the two opposing clamping units of the clamping device 24 move away from each other, and the valve control part 21 can gradually move away from the POCT reagent box 10, the film of the liquid distribution drive 15 can gradually deform to restore the negative pressure in the amplification cavity 14, the system liquid in the system cavity 13 can be driven to flow into the amplification cavity 14, and mix with the reagent in the amplification cavity 14 to form an amplification liquid, in this step, the valve control part 21 in the away state can release the magnetic attraction to the flow control valve 123 to seal the transfer flow channel between the buffer cavity 12 and the system cavity 13, so that the POCT reagent box 10 can realize timely valve sealing and maximize the reduction of the risk of pollution.

[0144] Figure 26 is a state diagram of the first drive motor 401 driving the hot melt bonding part 301 to close the amplification cavity 14; before the combination, the POCT reagent kit structure, the amplification cavity 14 can be in fluid communication with the corresponding distribution drive unit through the first flow channel 17 corresponding to the amplification cavity 14, and in fluid communication with the distribution flow channel through the second flow channel 18 corresponding to the amplification cavity 14; here the first drive motor 401 can drive the moving bracket 107 to move upwards, and then drive the hot melt bonding part 301 to approach and contact the POCT reagent kit 10 through the alternate transmission mechanism 22; here the hot melt bonding part 301 can output a contact temperature of 180℃-240℃, so that the film is hot melt connected with the reagent kit substrate to damage and close the first flow channel 17 and the second flow channel 18, so that the amplification cavity 14 is in a sealed state; here, if the temperature is too low, it will cause the seal to be not tight, and then lead to the risk of leakage, and if the temperature is too high, it may cause the film to heat shrink too much, or even partially carbonize, and then cause the problem of hot melt failure; in order to improve the reliability and success rate of the combination, the reagent kit substrate is also provided with a combination auxiliary part 1610, and the first flow channel 17 or the second flow channel 18 is arranged between the two combination auxiliary parts 1610, so that the first flow channel 17 and the second flow channel 18 connected corresponding to each amplification cavity 14 are separated; the combination auxiliary part 1610 can receive hot melt deformation, so that the stress and strain in the melting process are released in time, and the reliability of the hot melt combination is ensured. Figure 27 is a state diagram of the hot melt bonding part 301 away from the POCT reagent kit 10 after the combination is completed; the first drive motor 401 can drive the moving bracket 107 to move downwards, drive the detection and collection unit 26 to approach and contact the POCT reagent kit 10, and the hot melt bonding part 301 away from the POCT reagent kit 10 is not affected, and the waste heat of the hot melt bonding part 301 can be quickly discharged; in order to ensure the reliability of the closure, the hot melt bonding part 301 includes a configuration part with a gradually reduced cross section on the side close to the POCT reagent kit 10, such as a triangle and a cone, so that the hot melt bonding part 301 can quickly and reliably close the amplification cavity 14 in a smaller operation area.Figure 28 is a cross-sectional view of the detection and collection unit 26 compressing the amplification cavity 14 and cooperating with the thermal cycle part 23 to complete the amplification detection; the first drive motor 401 can drive the detection and collection unit 26 to contact the POCT reagent kit 10, the thermal cycle part 23 and the detection and collection unit 26 are arranged on both sides of the POCT reagent kit 10 in the thickness direction, the detection and collection unit 26 includes a protruding contact part 261, which can be compared to the protruding structure independently arranged on the pressure head in Embodiment One, the contact part 261 is configured as a spherical or spherical-like contact head, which can contact and compress the amplification cavity 14 of the POCT reagent kit 10 from one side in the thickness direction, so that the thermal cycle part 23 on the other side can more closely contact the cover film of the amplification cavity 14, improve the heat transfer conditions, and also make the central detection hole located at the center of the contact part 261 more closely fit the amplification cavity 14, reduce the adverse effects such as dispersion, the detection and collection unit 26 and the transceiving detection unit are connected by the optical fiber 2611 to reduce the light transmission loss and avoid the risk of interference such as crosstalk, in combination with Figure 29, the transceiving detection unit includes a plurality of emission units connected by the optical fiber 2611, a plurality of receiving units connected by the optical fiber 2611, and a multi-channel transceiving end 28, one end of the emission unit and the receiving unit is connected to the detection and collection unit 26, and the other end is connected to the corresponding optical fiber fixing hole 280 on the transceiving connection plate, a plurality of optical fiber fixing holes 280 are arranged in a linear interval along the length direction of the transceiving connection plate, the multi-channel transceiving end 28 can move back and forth along the length direction of the transceiving connection plate under the drive of the transceiving drive motor, and then the multi-channel transceiving end 28 can move and scan the plurality of through optical fiber fixing holes 280 arranged in a linear interval, to complete the emission and reception of light waves to obtain the amplification result in the amplification cavity 14. The transceiving drive motor can drive the multi-channel transceiving end 28 to move through belt wheel transmission, chain wheel transmission and other ways, and the transceiving drive motor drives the multi-channel transceiving end 28 to move and execute the scanning operation in the way of belt wheel transmission as shown in Figure 29.

[0145] The fluorescence detection module will be described in detail below in combination with the corresponding drawings, please refer to Figure 30 and Figure 31, Figure 30 is a schematic diagram of the connection structure of the optical fiber restraint part and the detection module in the fluorescence detection module provided by the present application, and Figure 31 is an exploded schematic diagram of the optical fiber restraint part in the fluorescence detection module provided by the present application, the fluorescence detection module further includes an optical fiber restraint part 29, the optical fiber restraint part 29 includes a limiting plate 291 fixedly connected with the compression part 200, a clamping plate 293 cooperating with the limiting plate 291, a flexible interlayer 292 arranged between the limiting plate 291 and the clamping plate 293, and the detection optical fiber 2611 is arranged in the flexible interlayer 292.

[0146] Preferably, the limiting plate 291 is in a bent configuration, which includes a connecting part fixed with the pressing part 200, and a limiting part matched with the clamping plate 293, that is, the horizontal connecting part connected with the pressing part 200 and the folded limiting part shown in FIG. 31, the middle section of the limiting part is bent at an angle range of 55°-85° with the middle section of the connecting part, in other words, the angle range of the middle section of the limiting part with the horizontal plane is 55°-85°, so that the detection optical fiber 2611 arranged in the optical fiber constraint part 29 does not have excessive mutation bending, the reliability of the fixed optical fiber is higher, and reliable fixation and constraint of the detection optical fiber can be realized, and even if the pressing part 200 moves, the light path and transmission efficiency of the detection optical fiber will not be affected, the limiting plate 291 with the set bending angle can maximize the change of the detection optical fiber path, and will not damage the optical fiber to ensure the reliability of use.

[0147] Preferably, the flexible interlayer 292 is two layers and is made of sponge, and the density of the sponge is 20-28 kg / m 3 The detection optical fiber is arranged in the flexible interlayer made of sponge, and is most preferably arranged between the two flexible interlayers 292, so that the detection optical fiber can be reliably fixed while basically having no risk of extrusion deformation damage.

[0148] The pressing part 200 provided by the embodiment includes six detection units, and the six detection units are arranged on the convex head structure of the pressing part 200. Specifically, each detection unit can include a spherical contact part, and a through hole is arranged at the center of the contact part to insert and fix the first end of the detection optical fiber 2611. The pressing part 200 is fixedly connected with the optical fiber constraint part 29, and the six detection optical fibers 2611 pass through the optical fiber constraint part 29. The detection optical fiber 2611 is at least partially fixed in the optical fiber constraint part 29, so that one end of the detection optical fiber 2611 can follow the movement of the pressing part 200. The optical fiber constraint part 29 can reliably clamp the detection optical fiber 2611, so that the movement of the detection module will not cause the detection optical fiber to bend and deform, and will not cause extrusion deformation.

[0149] Please refer to FIG. 32 and FIG. 33, FIG. 32 is a structure diagram of the transceiving detection module in the fluorescence detection module provided by the application, and FIG. 33 is a diagram of the transceiving detection module in FIG. 32, in which the multi-channel transceiving unit is driven to traverse and scan the fixed hole end of the detection optical fiber. The transceiving detection module comprises a transceiving substrate, a transceiving driving mechanism and a multi-channel transceiving unit 28. Specifically, the transceiving substrate is provided with optical fiber fixing holes 280, and the second end of the detection optical fiber 2611 is connected to the optical fiber fixing holes 280 on the transceiving substrate. In this embodiment, the transceiving substrate is provided with a plurality of through optical fiber fixing holes 280 arranged in a one-dimensional array, and the second end of the detection optical fiber 2611 is inserted into the optical fiber fixing holes 280. Adjacent optical fiber fixing holes 280 are arranged at a preset interval, and the interval distance can be 1.2-5 times the diameter of the optical fiber fixing hole 280. This is because too small a hole interval will cause the risk of detection light crosstalk and the hole strength will be weak, and too large a hole interval will result in reduced detection efficiency and control accuracy. The transceiving driving mechanism is arranged on one side of the transceiving substrate in the thickness direction, and the transceiving driving mechanism comprises a transceiving driving motor 151, the output of the transceiving driving motor 151 is connected to a transceiving driving wheel 152, a transceiving transmission wheel 154 is arranged at a preset interval from the transceiving driving wheel 152, and a transceiving transmission belt 153 is wound therebetween. One side of the transceiving transmission belt 153 is fixedly connected to a transceiving transmission block 156, and the transceiving transmission block 156 is also embeddedly connected to a transceiving sliding rail 155 fixedly arranged on the transceiving substrate. Optionally, the transceiving detection module further comprises a transceiving driving plate 157. The transceiving transmission block 156 is further connected to the transceiving driving plate 157 and the multi-channel transceiving unit 28, so that the transceiving driving motor 151 can drive the multi-channel transceiving unit 28 to slide, and then the multi-channel transceiving unit 28 can move to traverse and scan the plurality of through optical fiber fixing holes 280 arranged in a one-dimensional array, to complete the operations of emitting light waves and receiving light waves to obtain amplification results. Here, the multi-channel transceiving unit 28 scans the second end of the detection optical fiber to obtain the fluorescence results in the reaction cavity. The multi-channel transceiving unit 28 can comprise one or more basic units of any number of emission units and reception units spliced together, such as a combination of FAM, VIC, ROX and other detection channels, to achieve efficient multi-channel combination. Of course, in some special cases, the transceiving driving motor can also drive the multi-channel transceiving unit 28 to move through a chain wheel transmission mechanism or a screw sliding block transmission mechanism (instead of a transceiving belt wheel transmission mechanism) or other transceiving transmission mechanisms. In the fluorescence detection module provided in this embodiment, the detection optical fiber is optically connected to the detection module 11 and the transceiving detection module, so that the various functional modules of the entire fluorescence detection module can be separately arranged without interference in a limited space.

[0150] The fluorescence detection module provided in the embodiment receives the POCT kit through the kit receiving position, and the one side of the POCT kit in the thickness direction is in thermal connection with the thermal cycle part, and the other side can abut against the pressing part 200 and the hot melt combination part 301. When the composite driving mechanism drives the moving support 107 to move in the first direction or the second direction, the hot melt combination part 301 and the pressing part 200 are alternately moved horizontally to the position of the reaction cavity, so that the hot melt combination part 301 can heat and melt the fluid channel connected with the reaction cavity in the POCT kit to form a mutually independent and sealed reaction cavity. When the composite driving mechanism drives the moving support to move in the opposite direction, the pressing part 200 moves to the reaction cavity of the POCT kit and applies horizontal pressure, so that the reaction cavity is closely attached to the heat source, avoiding the uneven heating of the reaction cavity caused by the difference in surface flatness and insufficient extrusion force, and improving the efficiency of the PCR reaction. In addition, the fluorescence detection module in the pressing part 200 changes the fixing and installation mode of the detection optical fiber. The first end of the detection optical fiber is arranged in the pressing part 200 which can approach or move away from the reaction cavity of the kit. The detection optical fiber follows the pressing part 200 to perform amplification detection, and can perform efficient detection when the pressing part 200 presses the reaction cavity. The second end of the detection optical fiber is connected to the transceiving detection module, and can be scanned and detected by the movable multi-channel transceiving unit 28 to perform multi-target detection. By setting the optical fiber restraint part, the limiting plate is arranged in a bending structure and bent at a specific angle, the detection module is fixedly connected to the limiting plate, the clamping plate matched with the limiting plate is arranged, the flexible clamping layer is arranged between the limiting plate and the clamping plate, and the detection optical fiber is arranged in the flexible clamping layer. Even if the detection module moves, it will not affect the light path and transmission efficiency of the detection optical fiber. The limiting plate with a bending angle can maximize the change of the direction of the detection optical fiber, and will not damage the optical fiber to ensure the reliability of use. By fixing the detection optical fiber in the flexible clamping layer, especially between the multi-layer sponge clamping layer, the detection optical fiber can be reliably fixed without the risk of extrusion deformation damage, realizing reliable fixation and constraint of the detection optical fiber. The second end of the detection optical fiber is inserted into the optical fiber fixing hole arranged in a line on the transceiving substrate, and the multi-channel transceiving unit is driven by the transceiving driving mechanism to move and traverse each optical fiber fixing hole, realizing efficient and rapid detection. The closed multi-channel transceiving unit can shield the interference of external stray light, so that the detection result is accurate and reliable.

[0151] In order to ensure that the molecular diagnostic equipment provided with multiple cyclic amplification detection modules is more compact and reasonable, the second optical fiber restraint part 41 is further arranged in the molecular diagnostic equipment, which can restrain the detection optical fibers 2611 of different cyclic amplification detection modules. The constituent elements of the second optical fiber restraint part 41 are the same as those of the optical fiber restraint part 26 on the compression part 200. The second optical fiber restraint part 41 includes a limiting plate fixedly connected to the equipment and a clamping plate matched with the limiting plate. A flexible clamping layer is arranged between the limiting plate and the clamping plate. The detection optical fibers 1102 passing through one or more optical fiber fixing holes 280 are arranged in the flexible clamping layer. The second optical fiber restraint part 41 can restrain the detection optical fibers of different cyclic amplification detection modules (combined with the position of the structural member shown in FIG. 16).

[0152] The thermal cycle part provided by the present application is described in detail below in combination with the drawings. Please refer to FIG. 35 and FIG. 36. FIG. 35 is a schematic structural diagram of the thermal cycle part for the molecular diagnostic equipment provided by the present embodiment, and FIG. 36 is an exploded structural diagram of the thermal cycle part. The thermal cycle part for the molecular diagnostic equipment includes a base 2330, a heat conduction assembly, and a heat dissipation assembly.

[0153] Specifically, one side of the base 2330 is fixed with a heat source to form a heating part 233 capable of contacting and heating the POCT reagent kit, and the other side of the base 2330 is connected with a heat conduction assembly. The heat conduction assembly includes a heat transfer base 2332 connected with the base 2330 for heat transfer and a heat pipe heat exchanger 234 (herein, the heat pipe heat exchanger is taken as a heat pipe heat exchanger, which utilizes the evaporation and condensation of the cold end and the hot end to contain latent heat for efficient heat transfer), and the heat pipe heat exchanger 234 includes a group of pipe groups or multiple groups of spaced pipe groups, each group of pipe groups including a heat conduction part, a first heat dissipation part and a second heat dissipation part, the heat conduction part being embedded in the heat transfer base 2332, the first heat dissipation part penetrating through the heat dissipation fin group 2320 along the length direction of the heat dissipation fin group 2320 in the heat dissipation assembly to connect the first end of the heat conduction part, the second heat dissipation part penetrating through the heat dissipation fin group 2320 along the length direction of the heat dissipation fin group 2320 in the heat dissipation assembly to connect the second end of the heat conduction part, the penetrating direction of the first heat dissipation part in the heat dissipation fin group 2320 being different from the penetrating direction of the second heat dissipation part in the heat dissipation fin group 2320, or in other words, the main direction of the first heat dissipation part is different from the main direction of the second heat dissipation part. The heat dissipation assembly includes a fin heat dissipation unit 232 and a fan 231, the first end of the support 2321 in the fin heat dissipation unit 232 is fixed with the heat dissipation fin group 2320, the second end of the support 2321 is fixed with the fan 231, multiple heat dissipation fins are arranged along the length direction of the heat dissipation fin group 2320, each fin can be arranged in a substantially equal interval, or can be arranged in a horn-shaped non-equal interval structure, the heat pipe heat exchanger 234 penetrates through the multiple heat dissipation fins, and the fan 231, the heat dissipation fin group 2320 and the heat source are located on the same straight line, so that the fan 231 can directly dissipate heat for the heat dissipation fin group 2320 and the heat source. The heat cycle part for the molecular diagnostic equipment provided in the embodiment connects the heat source and the heat dissipation fin group through the heat conduction assembly, and arranges multiple heat pipe heat exchangers 234 including a heat conduction part and two heat dissipation parts, which not only increases the heat exchange area and improves the heat transfer efficiency, but also improves the utilization rate of the fan and the uniformity of heat dissipation of the heat source. The structural design of the heat pipe heat exchanger 234 fully utilizes the heat dissipation effect of the internal area of the heat dissipation fin group, and the position and direction design of the fan 231, the heat dissipation fin group 2320 and the heat source located on the same straight line makes the heat dissipation wind direction be on the best designed passage with higher freedom.

[0154] Further, in order to reduce the processing cost under the demand of basic heat transfer efficiency, the base 2330 is made of aluminum alloy material, and the heat transfer base 2332 and the heat pipe heat exchanger 234 are made of copper material, which improves the heat transfer efficiency while reducing the manufacturing cost of the heat cycle part.

[0155] In the embodiment, the base 2330 is a cuboid plate made of metal material, one side of the base 2330 is tightly fixed with a heating part 233 by screws, the heating part 233 is used to provide the required heat for PCR reaction, and the other side of the base 2330 is tightly fixed with a heat conduction assembly, the heat conduction assembly connects the base 2330 with a heat dissipation assembly, the heat conduction assembly can transfer the waste heat on the heat source to the heat dissipation assembly, thereby improving the heat exchange efficiency, so as to maintain the required temperature of the PCR instrument.

[0156] Preferably, the heat conduction assembly further comprises a heat conduction mounting plate 2331, the heat conduction mounting plate 2331 tightly connects the heat transfer base 2332 with the base 2330. In the embodiment, the heat transfer base 2332 is a cuboid metal plate with a certain heat capacity, the length, width and thickness of the heat transfer base 2332 are 60mm×34mm×5.5mm respectively. The heat transfer base 2332 is tightly fixed with the base 2330 through the heat conduction mounting plate 2331; specifically, the first side of the heat transfer base 2332 is adjacent to the base 2330, the heat conduction mounting plate 2331 is arranged on the second side opposite to the first side of the heat transfer base 2332 (in order to facilitate the display of each component, the heat conduction mounting plate 2331 is not shown on the side wall of the heat transfer base 2332 in FIG. 36), the heat conduction mounting plate 2331 tightly fixes the heat transfer base 2332 and the base 2330 together, the heat pipe heat exchanger 234 is connected between the heat transfer base 2332 and the heat dissipation fin group 2320, specifically, the heat dissipation fin group 2320 is provided with mounting holes capable of accommodating the heat pipe heat exchanger 234 at the interval positions of the heat dissipation fins, and the heat pipe heat exchanger is fixedly installed inside the heat dissipation fins by welding.

[0157] Further, each pipe group included in the heat pipe heat exchanger 234 comprises a heat conduction part and two heat dissipation parts, the heat conduction part is a first horizontal pipe penetrating through and embedded in the heat transfer base 2332, the first heat dissipation part comprises a first elbow pipe and a second horizontal pipe connected with each other, the second horizontal pipe penetrates through the heat dissipation fins along the length direction of the heat dissipation fin group 2320 and is connected with the first end of the first elbow pipe, the second end of the first elbow pipe is connected with the first end of the first horizontal pipe, the second heat dissipation part comprises a second elbow pipe and a third horizontal pipe connected with each other, the third horizontal pipe penetrates through the heat dissipation fins along the length direction of the heat dissipation fin group 2320 and is connected with the first end of the second elbow pipe, the second end of the second elbow pipe is connected with the second end of the first horizontal pipe, so that the heat conduction part and the two heat dissipation parts are integrally formed to form an integrated pipe group. In addition, the heat dissipation fin group 2320 is arranged opposite to the heat source, preferably, the heat dissipation fin group 2320 is arranged between the fan 231 and the heat conduction assembly.

[0158] Preferably, the second horizontal pipe and the third horizontal pipe are arranged in the heat dissipation fins in a penetrating manner along the length direction of the heat dissipation fin group 2320. Due to the different positions of the second horizontal pipe and the third horizontal pipe penetrating the heat dissipation fins, the contact area between the pipe group and the heat dissipation fins in the heat dissipation fin group 2320 is increased, and the heat dissipation efficiency of the internal area of the heat dissipation fin group 2320 is improved.

[0159] Preferably, when the pipe group is multiple, the adjacent pipe groups are arranged in a spaced manner. The heat exchange area is increased to improve the heat energy efficiency obtained by the heat pipe heat exchanger 234 from the heat transfer base 2332, and the heat exchange rate between the heat pipe heat exchanger 234 and the heat dissipation fin group 2320. In the embodiment, two pipe groups are arranged in a spaced manner. The gap between the heat dissipation fins in the heat dissipation fin group 2320 is opposite to the heat source. The heat cycle part provided in the embodiment is arranged to embed the heat conduction part in the heat transfer base 2332 along the length direction of the heat transfer base 2332. The contact area between the heat pipe heat exchanger 234 and the heat transfer base 2332 is increased, the heat energy efficiency obtained by the heat pipe heat exchanger 234 from the heat transfer base 2332 is improved, and at the same time, the two heat dissipation parts penetrate the heat dissipation fins along the length direction of the heat dissipation fin group 2320, the contact area between the heat pipe heat exchanger 234 and the heat dissipation fins in the heat dissipation fin group 2320 is increased, and the heat dissipation area inside the heat dissipation fin group 2320 is fully utilized.

[0160] Further, as shown in FIG. 37, the heat conduction part, the first heat dissipation part and the second heat dissipation part form a hollow structure, so that there is a gap between the base 2330 and the heat dissipation fin group 2320, so as to facilitate the installation of other structural parts of the PCR device in the gap between the base 2330 and the heat dissipation fin group 2320. The heat cycle part provided in the embodiment improves the compactness of the molecular diagnostic equipment structure, so that the space around the heat source is released, the installation of other components is facilitated or the movement of other components is facilitated, the utilization rate of fan air volume is improved, and the heat dissipation needs of the miniaturized and integrated device are met. In the embodiment, one horizontal pipe of the heat pipe heat exchanger 234 is embedded in the heat transfer base 2332 and extends out from the two side walls of the heat transfer base 2332 respectively. In order to increase the heat exchange area, the first horizontal pipe embedded in the heat transfer base 2332 is connected to the second horizontal pipe and the third horizontal pipe through the elbow pipe at both ends respectively, and the second horizontal pipe and the third horizontal pipe penetrate the heat dissipation fin group 2320 in a spaced manner. In addition, the shape of the heat pipe heat exchanger 234 can also be linear or U-shaped, and the specific bending direction can be set according to the actual design channel, so as to improve the design freedom of the device. In other embodiments, the horizontal pipe and the elbow pipe can also be other numbers.

[0161] Preferably, the heat pipe heat exchanger 234 is filled with inert gas medium or heat conductive fluid. The heat pipe heat exchanger 234 can be filled with gas or heat conductive liquid, such as water or alcohol, to further enhance the heat transfer efficiency.

[0162] Further, the length direction of each heat dissipation fin is perpendicular to the length direction of the heat dissipation fin group 2320, the outlet of the fan 231 is opposite to the gap between adjacent heat dissipation fins in the heat dissipation fin group 2320, and the fan 231 can directly dissipate the heat energy collected in the gap between the heat dissipation fins after blowing, thereby improving the heat dissipation efficiency.

[0163] Further, the center of the bracket 2321 is hollow, so that the airflow generated by the fan 231 can directly enter the gap between adjacent heat dissipation fins. Specifically, in order to enhance the heat dissipation efficiency, the outlet of the fan 231 is opposite to the gap between the heat dissipation fins in the heat dissipation fin group 2320. Since the center of the bracket 2321 is hollow, the airflow generated by the fan 231 directly contacts the heat dissipation fin group 2320 through the hollow structure, so that the heat dissipation effect between the fan 231 and the heat dissipation fin group 2320 and the heat pipe heat exchanger 234 is good.

[0164] In the embodiment, the size of the fan is 60mmx60mm, and the power is 12W. As shown in FIG. 38, the fan 231 is fixedly installed on the outside of the bracket 2321 by screws, and a plurality of heat dissipation fins are vertically fixed on the inside of the bracket 2321 opposite to the base 2330. There is a gap between the base 2330 and the heat dissipation fin group 2320, so as to facilitate the installation of additional structural members of the PCR instrument around the heat source 101, improve the structural compactness of the PCR instrument device, and facilitate the utilization of the fan air volume. The local heat dissipation device for the miniaturized PCR instrument provided in the embodiment can achieve an average cooling rate of 8℃ / s.

[0165] Embodiment Four

[0166] The operation method of the molecular diagnostic equipment cooperating with the embodiment three comprises the following steps:

[0167] Pre-operation step: placing the POCT kit in the kit receiving position 201 of the molecular diagnostic equipment;

[0168] Drive preparation step: driving the clamping device 24 to move towards the POCT kit until the clamping device compresses the dispensing driving part 15, the dispensing driving part 15 generates elastic deformation, the air pressure in the amplification cavity increases, the liquid flow from the system cavity 13 to the amplification cavity 14 is limited, the valve control part 21 is close to and acts on the flow control valve 123, the flow control valve 123 is opened, the buffer cavity 12 and the system cavity 13 are communicated, and the valve control part 21 and the clamping device 24 at least partially overlap during movement;

[0169] Air source driving step: the air source driving part 25 is started, the air pressure in the buffer cavity 12 is changed, the liquid is circulated between the buffer cavity 12 and the system cavity 13, and after the liquid is fully mixed, the liquid is stored in the system cavity 13;

[0170] Liquid separation driving step: the clamping device 24 is driven to release the liquid separation driving part 15, the liquid separation driving part 15 is gradually deformed to restore the negative pressure in the amplification cavity 14, and the liquid in the system cavity 13 is driven to flow into the amplification cavity 14 under the action of the negative pressure until the filling of the amplification cavity 14 is completed.

[0171] Further comprising an amplification cavity sealing step: driving the hot melt bonding part 301 to contact the POCT reagent kit, so that the filled amplification cavity 14 is sealed by hot melt bonding;

[0172] Amplification detection step: driving the pressing part 200 to move towards the amplification cavity 14 of the POCT reagent kit until the pressing part 200 drives the detection and collection unit 26 to contact and press the amplification cavity 14, starts the thermal cycle part 23 to apply constant temperature or variable temperature to the amplification cavity 14, makes the liquid in the amplification cavity produce amplification reaction, drives the detection part to detect the amplification system liquid in the amplification, and obtains the amplification detection result.

[0173] Among them, the thermal cycle part 23 applies variable temperature thermal cycle to the amplification cavity 14, the amplification cavity 14 is in close contact with the heat source, and one side of the heat source Peltier element is used for temperature rising / lowering operation of the amplification cavity 14;

[0174] The temperature rising / lowering operation comprises a high temperature denaturation sub-step: in the temperature rising stage, the heat source starts to transfer heat to the amplification cavity 14, so that the temperature of the amplification cavity 14 can be raised and maintained at 90-97℃ for a short time, so as to realize high temperature denaturation of the to-be-tested amplification reaction liquid;

[0175] Low temperature annealing sub-step: in the low temperature annealing stage, the heat source needs to be cooled to 45-55℃, the heat source working mode is changed to a cooling stage assisted by refrigeration, in order to facilitate rapid cooling, the fan is started at this time, the heat generated by the lower surface of the heat source is rapidly diffused and conducted to the heat pipe radiator through the heat transfer base, the heat transfer medium in the heat pipe radiator absorbs the heat and directly transfers the heat to the heat dissipation fin group, and forced convection heat dissipation is carried out under the action of the fan, because the heat source, the heat transfer base, the heat dissipation fin group and the fan are in the same direction, and there is a certain gap distance between the heat dissipation fin group and the heat transfer base, so that the airflow generated by the fan can be used to a greater extent, and the heat exchange efficiency is improved.

[0176] Extension sub-step: compared with the annealing low temperature, the extension needs 72℃, at this time the heat source continues to heat and work to raise the temperature, so that the amplification reaction liquid in the amplification cavity 14 is heated and maintained at the required temperature condition for a short time, and all amplification reactions are completed.

[0177] Of course, the above describes the three-step amplification reaction, and in actual use, a two-step method can also be used to perform thermal cycle amplification, and the overall mechanism is similar, which will not be repeated here.

[0178] The principles and implementation manners of the present application are described by using specific examples in the present application, and the above examples are only used to help understand the method of the present application and its core idea. It should be pointed out that, for ordinary skilled in the art, without departing from the principles of the present application, the present application can be improved and modified in several ways, and these improvements and modifications also fall within the protection scope of the claims of the present application.

[0179] In the description of the present application, it should be pointed out that the orientations or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like are based on the orientations or positional relationships shown in the drawings, or the orientations or positional relationships in which the product of the present application is usually placed, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0180] In the description of the present application, it should be pointed out that, unless otherwise explicitly specified and limited, the terms "arrangement", "installation", "connection", "connection" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication inside two elements. For ordinary skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

Claims

1. A pinch seal device for a POCT cartridge, characterized in that, The fixed clamping part comprises a fixed clamping part, a pressing part, a sealing part and a transmission assembly, wherein, The fixed clamping part comprises a reagent box receiving site and a moving support, the reagent box receiving site can clamp the POCT reagent box, the transmission assembly can drive the moving support to move in the first direction or the second direction, so that the moving support drives the pressing part and the sealing part to alternately contact or move away from the POCT reagent box, the sealing part seals the reaction chamber of the POCT reagent box when it contacts the POCT reagent box, and the pressing part makes the reaction chamber of the POCT reagent box closely contact with the heat source when it contacts the POCT reagent box.

2. The pinch seal device for a POCT cartridge of claim 1, wherein, The transmission assembly is respectively connected with the pressing part and the sealing part, the pressing part is movably connected with the moving support, and the sealing part is movably connected with the moving support.

3. The pinch seal device for a POCT cartridge of claim 1, wherein, The fixed clamping part further comprises a fixed table, a first guide rod and a second guide rod, the fixed table is located above the moving support, the fixed table is connected with the clamping part, the first guide rod and the second guide rod both penetrate through the fixed table and are movably connected with the fixed table, one end of the first guide rod close to the POCT reagent box is connected with the sealing part, one end of the second guide rod close to the POCT reagent box is connected with the pressing part, when the moving support moves in the first direction, the sealing part can move along the first guide rod to approach the POCT reagent box, and the pressing part can move along the second guide rod to move away from the POCT reagent box, when the moving support moves in the second direction, the sealing part can move along the first guide rod to move away from the POCT reagent box, and the pressing part can move along the second guide rod to approach the POCT reagent box.

4. The pinch seal device for a POCT cartridge of claim 3, wherein, The fixed clamping part further comprises a mounting plate, a first rotating rod, a second rotating rod, a first connecting rod and a second connecting rod, the mounting plate is fixed on the transmission assembly, the mounting plate is provided with a stand column, the stand column is distributed on both sides of the moving support, the first connecting rod penetrates through the first connecting holes on the stand columns on both sides of the moving support, the second connecting rod penetrates through the second connecting holes on the stand columns on both sides of the moving support, the moving support is provided with a pressing part movable hole and a sealing part movable hole, the second rotating rod penetrates through the pressing part movable hole, and the first rotating rod penetrates through the sealing part movable hole, both sides of the moving support are symmetrical, on the first side of the moving support, the first connecting rod is movably connected with the first rotating rod, the first rotating rod is movably connected with the sealing part, the second connecting rod is movably connected with the second rotating rod, and the second rotating rod is movably connected with the pressing part, when the transmission assembly drives the moving support to move, the first rotating rod drives the first connecting rod to move, the sealing part moves away from or approaches the POCT reagent box, the second rotating rod drives the second connecting rod to move, and the pressing part approaches or moves away from the POCT reagent box.

5. The pinch seal device for a POCT cartridge of claim 4, wherein, The first connecting rod is hinged with the first rotating rod to form a first connecting part, the first rotating rod is hinged with the sealing part to form a second connecting part, the first connecting part and the second connecting part form an included angle in the range of [85°-180°) in the clockwise direction, the second connecting rod is hinged with the second rotating rod to form a third connecting part, the second connecting rod is hinged with the pressing part to form a fourth connecting part, and the third connecting part and the fourth connecting part form an angle in the range of [85°-180°) in the counterclockwise direction.

6. A molecular diagnostic device, characterized by The compact sealing device for the POCT kit comprises a buffer cavity for receiving an external liquid, a system cavity for dispensing the liquid, an amplification cavity for performing detection, and a liquid distribution driving part for controlling the air pressure in the amplification cavity, the buffer cavity and the amplification cavity are in fluid communication with the system cavity, the system cavity is in communication with the external environment through a gas return port, a flow control valve is arranged between the buffer cavity and the amplification cavity to control the on-off relationship, and the liquid distribution driving part can be elastically deformed under the action of an external force and restored without the action of the external force. The device body comprises a plurality of reagent kit receiving parts for receiving POCT kits and performing amplification detection, each reagent kit receiving part comprises a reagent kit receiving position, a clamping device for adjusting the pressure of the system cavity to control the on-off relationship between the system cavity and the amplification cavity, a valve control part for controlling the on-off relationship of the flow control valve, and a liquid distribution driving part for communicating and controlling the air pressure in the buffer cavity; the valve control part and the clamping device are movably connected to the device body, the valve control part can be moved to the position of the flow control valve corresponding to the reagent kit receiving position and can drive the flow control valve to open, and the valve control part can be moved away from the reagent kit receiving position to close the flow control valve; the clamping device can be moved to the position of the liquid distribution driving part corresponding to the reagent kit receiving position and can press the liquid distribution driving part to increase the air pressure in the amplification cavity, and the clamping device can be moved away from the reagent kit receiving position to reduce the air pressure in the amplification cavity; the valve control part and the clamping device at least partially overlap when moving.

7. The molecular diagnostic device of claim 6, wherein, The clamping device can press the liquid distribution driving part under the action of the second driving part, and the valve control part is connected to the clamping unit through a transmission block and follows the movement of the clamping unit.

8. The molecular diagnostic device of claim 7, wherein, The clamping device comprises a pair of clamping units arranged opposite to each other on both sides of the reagent kit receiving position, and the pair of clamping units can be driven by the second driving part to approach each other and press the liquid distribution driving part from both sides in the thickness direction of the POCT kit; the second driving part comprises a second driving motor and a transmission screw, the transmission screw is provided with a right-hand thread segment and a left-hand thread segment, and the pair of clamping units are threadedly connected to the right-hand thread segment and the left-hand thread segment of the transmission screw, respectively.

9. The molecular diagnostic device of claim 6, wherein, The reagent kit receiving part further comprises a thermal cycling part for amplifying the POCT kit and a detection part for detection, and the detection collection units of the thermal cycling part and the detection part are arranged on opposite sides of the reagent kit receiving position.

10. The molecular diagnostic device of claim 6, wherein, The air source driving part comprises an air source driving member and an air source connecting pipe, and the buffer cavity is communicated with the air source connecting pipe through an air inlet; the kit receiving part is provided with a receiving opening for receiving the POCT kit, and a closable receiving cover is arranged at the receiving opening; the receiving cover is provided with an air source interface, one side of the air source interface is in communication with the air source connecting pipe, and the other side is matched with the air inlet of the POCT kit in the kit receiving position; and the side of the air source interface matched with the air inlet is provided with a rubber pad for sealing and pressing the air inlet.

11. The molecular diagnostic device of claim 6, wherein, The sealing part further comprises a hot melt joint part for destructive sealing of the amplification cavity, the device body is provided with a device air inlet at the front end and a device air outlet at the rear end, the thermal cycle part is arranged on one side close to the device air outlet, and the hot melt joint part and the detection and collection unit are arranged on one side close to the device air inlet.

12. The molecular diagnostic device of claim 6, wherein, Further comprising a detection module and a transceiving detection module, wherein the sealing part comprises a hot melt joint part, the hot melt joint part seals the amplification cavity of the POCT kit when in contact with the POCT kit, the pressing part is further connected to the detection module, the detection module is provided with a detection optical fiber, and the detection module is in contact with the POCT kit, so that the reaction cavity of the POCT kit is tightly fitted between the first end of the detection optical fiber and the device heat source, the second end of the detection optical fiber is connected to the transceiving detection module, the transceiving detection module comprises a multi-channel transceiving unit capable of being driven to move, and the multi-channel transceiving unit scans the second end of the detection optical fiber to obtain real-time fluorescence results in the reaction cavity.

13. The molecular diagnostic device of claim 12, wherein, The detection module comprises a plurality of protruding detection units, the detection unit further comprises a detection hole penetrating through the columnar protruding structure, and the detection hole is configured to connect the detection optical fiber.

14. The molecular diagnostic device of claim 12, wherein, Further comprising a fiber constraint part, the fiber constraint part comprises a limiting plate fixedly connected with the detection module, a clamping plate matched with the limiting plate, and a flexible interlayer arranged between the limiting plate and the clamping plate, and the detection optical fiber is arranged in the flexible interlayer.

15. The molecular diagnostic device of claim 14, wherein, The limiting plate is of a bending configuration, which comprises a connecting part fixed with the detection module, a limiting part cooperatively connected with the clamping plate, and a middle cross section of the limiting part and a middle cross section of the connecting part are bent at an angle ranging from 55° to 85°, and the material of the flexible clamping layer is sponge with a density ranging from 20 to 28 kg / m 3 .

16. The molecular diagnostic device of claim 12, wherein, The transceiving detection module comprises a transceiving substrate, a transceiving driving mechanism, a transceiving driving plate, and a multi-channel transceiving unit, the transceiving substrate is provided with a fiber fixing hole, the second end of the detection optical fiber is connected to the fiber fixing hole, one side of the transceiving substrate in the thickness direction is provided with the transceiving driving mechanism, the transceiving driving mechanism comprises a transceiving driving motor, the transceiving driving motor is connected with a transceiving driving wheel, a transceiving transmission wheel is arranged at a preset interval from the transceiving driving wheel, a transceiving transmission belt is wound between the transceiving driving wheel and the transceiving transmission wheel, one side of the transceiving transmission belt is fixedly connected with a transceiving transmission block, the transceiving transmission block is also embeddedly connected to a transceiving slide rail fixedly arranged on the transceiving substrate, and the transceiving transmission block is further connected with the transceiving driving plate and the multi-channel transceiving unit; the transceiving driving motor can drive the multi-channel transceiving unit to slide, so that the multi-channel transceiving unit can move and scan the fiber fixing hole.

17. The molecular diagnostic device of claim 6, wherein, Further comprising a thermal cycle part, the thermal cycle part comprises a base, a heat conduction component, and a heat dissipation component, one side of the base is fixed with a heat source, and the other side of the base is connected with the heat conduction component; The heat conducting assembly comprises a heat conducting base and a heat pipe heat exchanger, the heat conducting base is connected with the base for heat transfer, the heat pipe heat exchanger comprises a group of pipe groups or multiple groups of spaced pipe groups, each group of pipe groups comprises a heat conducting part, a first heat radiating part and a second heat radiating part, the heat conducting part is embedded in the heat conducting base, the first heat radiating part penetrates through the heat radiating fin group along the length direction of the heat radiating fin group and is connected with the first end of the heat conducting part, the second heat radiating part penetrates through the heat radiating fin group along the length direction of the heat radiating fin group and is connected with the second end of the heat conducting part; the penetrating direction of the first heat radiating part in the heat radiating fin group is different from the penetrating direction of the second heat radiating part in the heat radiating fin group; the heat radiating assembly comprises a fin heat radiating unit and a fan.

18. The molecular diagnostic device of claim 17, wherein, The first end of the support is fixed with the heat radiating fin group, the second end of the support is fixed with the fan, multiple heat radiating fins are arranged along the length direction of the heat radiating fin group, the heat pipe heat exchanger penetrates through the multiple heat radiating fins, the fan is located in the same straight line position with the heat radiating fin group and the heat source, so that the fan can directly radiate heat for the heat radiating fin group and the heat source.

19. A method of operating a molecular diagnostic apparatus using the molecular diagnostic apparatus according to claim 6, characterized by, The method comprises the following steps: The pre-operation step is that the POCT reagent box is placed in the reagent box receiving position of the molecular diagnostic equipment; The driving preparation step is that the clamping device is driven to move to the POCT reagent box until the clamping device compresses the separation driving part, the separation driving part produces elastic deformation, the air pressure in the amplification cavity is increased, the liquid is limited to flow from the system cavity into the amplification cavity, the valve control part is close to and acts on the flow control valve, the flow control valve is opened, the buffer cavity is communicated with the system cavity, and at least part of the time periods of the movement of the valve control part and the clamping device overlap; The gas source driving step is that the gas source driving part is started to change the air pressure in the buffer cavity, the liquid is circulated between the buffer cavity and the system cavity, after the liquid is fully mixed, the liquid is stored in the system cavity; The separation driving step is that the clamping device is driven to release the separation driving part, the separation driving part gradually recovers to produce negative pressure in the amplification cavity, the liquid in the system cavity is driven to flow into the amplification cavity under the action of the negative pressure, and the filling of the amplification cavity is completed.

20. The method of operating a molecular diagnostic apparatus of claim 19, wherein, The amplification detection step is that the thermal cycle part is started to apply constant temperature or variable temperature to the amplification cavity, the liquid in the amplification cavity produces an amplification reaction, the detection part is started to detect the amplification system liquid in the amplification, and an amplification detection result is obtained.

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