Complex protein glutaminase
By introducing a thrombin recognition site into the pro-sequence of protein glutaminase, a complex form is created that maintains or enhances thermal stability and oxidation resistance, addressing the challenges of recombinant expression systems.
WO2025239456A1PCT designated stage Publication Date: 2025-11-20AMANO ENZYME INC
Patent Information
- Application Number
- PCT/JP2025/017938
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2025-02-06
- Filing Date
- 2025-05-16
- Publication Date
- 2025-11-20
Smart Images
Figure JPOXMLDOC01-APPB-M000006 
Figure JPOXMLDOC01-APPB-M000007 
Figure JPOXMLDOC01-APPB-T000001
Abstract
A purpose of the present invention is to provide a recombinant expression technique for producing a protein glutaminase in which decreases in thermostability are suppressed. A complex protein glutaminase according to the present invention is a thrombin cleavage product of a mutant propolypeptide comprising a propolypeptide sequence which includes a pro-sequence and a mature sequence of protein glutaminase, wherein a thrombin recognition sequence is introduced into a C-terminal region of the pro-sequence. The complex protein glutaminase includes: (A) a pro-sequence peptide having an N-terminal cleavage fragment of the thrombin recognition sequence at the C-terminal thereof; and (B) a mature protein glutaminase having a C-terminal cleavage fragment of the thrombin recognition sequence at an N-terminal thereof. Said complex protein glutaminase has an improved thermal denaturation temperature compared to non-complex protein glutaminases.
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Citation Information
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