Interferon α2b mutant, fusion polypeptide comprising same, and use

By designing an affinity-weakened IFNα2b mutant fused with an anti-PD-1 antibody, the off-target toxicity and insufficient therapeutic window of IFNα2b drugs were resolved, achieving selective differentiation on PD-1 positive cells and higher tumor treatment efficacy.

WO2026138873A1 Publication Date: 2026-07-02INNOVENT BIOLOGICS (SUZHOU) CO LTD +1
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Patent Information

Application Number
PCT/CN2025/145113
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2025-07-11
Filing Date
2025-12-24
Publication Date
2026-07-02

AI Technical Summary

Technical Problem

Existing IFNα2b drugs exhibit off-target toxicity and non-specific inflammatory responses in tumor treatment, and the therapeutic window of the fusion protein TAK-573 of IFNα2b and anti-CD38 antibody needs to be improved.

Method used

By designing an affinity-weakened IFNα2b mutant and fusing it with an anti-PD-1 antibody, the binding to cell surface antigens is reduced by mutation or sequence substitution at specific sites, thereby reducing off-target toxicity and preserving activity against PD-1 positive cells.

Benefits of technology

It achieved selective differentiation between PD-1 positive and negative cells, reduced off-target toxicity, improved the therapeutic window, and demonstrated tumor-killing effects and higher tolerability in vivo.

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Abstract

An interferon α2b mutant, and a fusion polypeptide and polypeptide construct comprising same. The fusion polypeptide or polypeptide construct comprising the interferon α2b mutant has weakened affinity to an interferon receptor, reduced off-target toxicity, and a tumor killing effect.
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