Bacillus strains having pesticidal activity and use thereof
Novel Bacillus strains and seaweed extracts enhance the control of plant pathogens by providing synergistic pesticidal activity against nematodes, fungi, and insects, addressing the limitations of chemical pesticides.
Patent Information
- Authority / Receiving Office
- AU · AU
- Patent Type
- Applications
- Current Assignee / Owner
- ADAMA MAKHTESHIM LTD
- Filing Date
- 2023-12-21
- Publication Date
- 2026-07-09
AI Technical Summary
There is a need for safe and effective biocontrol agents to protect plants from plant pests such as nematodes, fungi, and insects, as chemical pesticides pose environmental and health hazards and lead to pest resistance.
Utilizing novel Bacillus velezensis and Bacillus subtillis strains, or their functional homologs, with specific 16S-rRNA sequences, to inhibit or kill plant pathogens, optionally combined with seaweed extracts for enhanced pesticidal activity.
The bacterial strains demonstrate additive or synergistic effects in controlling plant pathogens, increasing fungicidal, nematocidal, and insecticidal efficacy by up to 300% when combined, and can be formulated into agricultural compositions for various applications.
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Abstract
Description
FIELD OF THE INVENTION The present invention is in the field of biocontrol of plant pathogens by bacteria. Particularly, the present invention discloses Bacillus strains and combinations of Bacillus species effective as pesticides, particularly in killing or inhibiting the development of nematodes and / or fungi and / or insects and / or in protecting plants from deleterious effects of nematodes, fungi and / or insects, and use thereof, optionally with a seaweed extract and / or at least one pesticide for reducing deleterious effects of said pests on plants. BACKGROUND OF THE INVENTION Plants are exposed to multiple biotic and abiotic stress conditions along their entire growth period. In the production of crop plants, damages caused by biotic stresses, which may be further enhanced under conditions of abiotic stress, pose a major problem and significantly affect the crop yield and profitability. Pests including nematodes, fungi and insects are a major factor in the loss of agricultural crops worldwide. Nematodes (order Tylenchida), include many plant-parasitic nematodes that are active, flexible, elongate organisms living on moist surfaces or in liquid environments, including films of water within soil and moist tissues within other organisms. There are numerous plant-parasitic nematode species, including various cyst nematodes (e.g., Heterodera sp.), root knot nematodes (e.g., Meloidogyne sp.), lesion nematodes (e.g., Pratylenchus sp.), reniform nematodes (Rotylenchulus reniformis) dagger nematodes (e.g., Xiphinema sp.) and stem and bulb nematodes (e.g., Ditylenchus sp.), among others. Nematode species grow through a series of lifecycle stages and molts. Typically, there are five stages and four molts: egg stage; first juvenile stage - JI; first molt - Ml; second juvenile stage, sometimes hatch from egg- J2; M2; J3; M3; J4; M4; and adult-A. Juvenile ("J") stages are also sometimes referred to as larval ("L") stages. Nematode parasites of plants can inhabit all parts of plants, including roots, developing flower buds, leaves, and stems. Plant parasites are classified on the basis of their feeding habits into the broad categories of migratory ectoparasites, migratory endoparasites, and sedentary endoparasites. Sedentary endoparasites, which include the root knot nematodes (Meloidogyne) and cyst nematodes (Globodera and Heteroderci) induce feeding sites ("syncytia") and establish long-term infections within roots that are often very damaging to crops. Insects destroy economically valuable plants (including crops, trees and ornamental plants) by feeding on the plants either while in larval stage or as adults. Plant insect pests are of a variety of forms, belonging to different orders including Lepidoptera, Coleopterci, Hemiptera and more. Fungal and Oomycetous pathogens can cause significant damage to a wide variety of commercially important plant varieties including crops and ornamental plants. Such pathogens can kill plants, reduce yield, reduce plant strength (e.g., decrease resistance to lodging), cause symptoms of mineral deficiency, and predispose plants to infection by other pathogens. Most widely used are chemical pesticides, at a cost of millions of dollars every year in the US only. In recent years, awareness of the hazardous effect of chemical pesticides on the environment and on human and animal health brought regulators to ban or limit the use of some of the more hazardous pesticides that were traditionally employed in plant fields. In addition, emerging pest resistance issues stimulated the research for and development of alternative systems and compounds for controlling the damage caused by plant pests. Using microbial biocontrol agents (microbials), such as bacteria, or one or more products thereof, to combat the damages caused by plant pests is a developing, new approach. These biocontrol agents are applied to the plant or to a part thereof (e.g., to the seed), or to the plant habitat (e.g., to the soil, in furrow or by broadcasting) to control the pathogen. Microbials are good candidates to replace hazardous pesticides. One of the most commonly used insecticidal bacteria today are Bacillus species, since it was discovered that Bacillus thuringiensis (Bt) strains show a high toxicity against specific insects. Plant growth-promoting rhizobacteria (PGPR) and root-colonizing bacteria that enhance plant growth, have been also suggested as biocontrol agents against multiple plant pathogens. Bacillus was the major genus initiating a nematode mortality percentage greater than the other genera examined bacteria (Xiang, N., Lawrence, K. S., Kloepper, J. W., Donald, P. A., Mclnroy, J. A. and Lawrence, G. W. 2017. Biological control of Meloidogyne incognita by spore-forming plant growth-promoting rhizobacteria on cotton. Plant Disease 101:774-784). There is a continuous need for, and it would be highly advantageous to have, biocontrol agents, including alternative bacterial agents, to be used against plant pesticides. SUMMARY OF THE INVENTION The present invention answers the need for safe biocontrol agents that can protect plants, particularly crop plants, from plant pests including nematodes, fungi and certain insects. The present invention provides novel Bacillus bacterial strains having pesticidal activity, particularly against plant nematodes and fungi, and also against certain plant pathogenic insects. The strains of the invention may also be used in combination, having at least additive and preferably, a synergistic activity. The present invention further provides preparations of the bacterial strains, lysates, extracts, compositions comprising same and uses thereof. In certain aspects the present invention provides methods for conferring or enhancing the resistance of plants towards nematodes, fungi and / or insects, comprising contacting the plants, or the plants’ immediate surroundings, with the bacterial strain, combinations of bacterial strains and / or preparations thereof. According to certain aspects, the present invention provides an isolated bacterial strain or a functional homolog thereof, wherein the isolated bacterial strain is selected from the group consisting of: (a) a Bacillus velezensis strain ORN14, the strain being selected from the group consisting of: i. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO: 1; ii. a strain deposited under ATCC Accession Number PTA-127479; and a combination thereof; and (b) a Bacillus subtillis strain ORN160, the strain being selected from the group consisting of: i. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:2; ii. a strain deposited under ATCC Accession Number PTA-127478; and a combination thereof. It is to be explicitly understood that the species of a bacterial strain of the invention as described herein is classified to the closest known species according to said species characterization as is known in the art. According to certain embodiments, the functional homolog of strain 0RN14 comprises a 16S-rRNA sequence at least 97% identical to SEQ ID NO:1. According to some embodiments, the functional homolog of strain 0RN14 comprises a 16S-rRNA sequence at least 97.5%, at least 98%, at least 98.5%, at least 99%, or at least 99.5% identical to SEQ ID NO: 1. According to certain embodiments, the functional homolog of strain ORN160 comprises a 16S-rRNA sequence at least 97% identical to SEQ ID NO:2. According to some embodiments, the functional homolog of strain ORN160 comprises a 16S-rRNA sequence at least 97.5%, at least 98%, at least 98.5%, at least 99%, or at least 99.5% identical to SEQ ID NO:2. According to certain embodiments, each of strain 0RN14 and ORN160 is further characterized by at least one strain-specific genomic marker sequence. According to certain embodiments, each of the Bacillus velezensis strain 0RN14, the Bacillus subtillis strain ORN160, functional homolog thereof and bacterium derived therefrom is characterized by a capability to inhibit the growth and / or the development and / or the activity of, or to kill, at least one plant nematode, and or plant pathogenic fungus, and / or insect plant pest, thereby protecting the plant from, or reducing the impact of the harmful effects of, the at least one pest. The inhibitory activity or killing of the pest can be exerted directly by the bacterial strain, or be mediated by the plant, part thereof or the plant habitat affected by the bacterial strain. According to some particular embodiments, the Bacillus velezensis strain ORN14, functional homolog thereof and bacterium derived therefrom is characterized by a capability to inhibit the growth and / or the development and / or the activity of, or to kill, at least one plant pathogenic fungus, thereby protecting the plant from, or reducing the impact of the harmful effects of, the at least one plant pathogenic fungus. According to additional particular embodiments, the Bacillus velezensis strain 0RN14, functional homolog thereof and bacterium derived therefrom is characterized by a capability to inhibit the growth and / or the development and / or the activity of, or to kill, at least one plant pathogenic nematode, thereby protecting the plant from, or reducing the impact of the harmful effects of, the at least one plant pathogenic nematode. The inhibitory activity or killing of the pest can be exerted directly by the bacterial strain, or be mediated by the plant, part thereof or the plant habitat affected by the bacterial strain. According to some particular embodiments, the Bacillus subtillis strain ORN160, functional homolog thereof and bacterium derived therefrom is characterized by a capability to inhibit the growth and / or the development and / or the activity of, or to kill, at least one plant pathogenic nematode, thereby protecting the plant from, or reducing the impact of the harmful effects of, the at least one plant pathogenic nematode. The inhibitory activity or killing of the pest can be exerted directly by the bacterial strain, or be mediated by the plant, part thereof or the plant habitat affected by the bacterial strain. It is to be explicitly understood that the present invention encompasses a bacterium of the bacterial strains or the functional homolog strains thereof, as well as a bacterium derivable from bacterial strains or from the functional homolog strains thereof. According to yet additional certain aspects, the present invention provides a microbial combination comprising (i) Bacillus velezensis strain 0RN14 and / or functional homolog thereof; and (ii) Bacillus subtillis strain ORN160 and / or functional homolog thereof, wherein the combination has an enhanced capability to inhibit the growth and / or the development and / or the activity of, or to kill, at least one plant nematode; and / or plant pathogenic fungus; and / or insect plant pest compared to the capability of each strain alone. According to certain embodiments, the combination has an additive capability to inhibit the growth and / or the development and / or the activity of, or to kill, at least one plant nematode; and / or plant pathogenic fungus; and / or insect plant pest compared to the capability of each strain alone. According to certain additional embodiments, the combination has a synergistic capability to inhibit the growth and / or the development and / or the activity of, or to kill, at least one plant nematode; and / or plant pathogenic fungus; and / or insect plant pest compared to the capability of each strain alone. According to certain embodiments, the combination comprises the Bacillus velezensis strain 0RN14 and the Bacillus subtillis strain ORN160 at a ratio of from about 10:1 to about 1:10. According to certain embodiments, the combination comprises the Bacillus velezensis strain 0RN14 and the Bacillus subtillis strain ORN160 at a ratio of 1:1. The microbial strains are as described hereinabove. According to another aspect, the present invention provides a bacterial preparation comprising a plurality of bacteria of at least one bacterial strain selected from the group consisting of Bacillus velezensis strain ORN14, Bacillus subtillis strain ORN160, functional homolog thereof and a combination of same, wherein the bacterial preparation is characterized by a pesticidal activity against at least one plant nematode, and / or plant pathogenic fungus, and / or insect plant pest. According to some embodiments, the bacterial preparation is characterized by a nematocidal activity. According to further embodiments, the bacterial preparation is characterized by a fungicidal activity. According to additional embodiments, the bacterial preparation is characterized by an insecticidal activity. According to some particular embodiments, the bacterial preparation of the present invention is characterized by both fungicidal and nematocidal activity. According to certain embodiments, the bacterial preparation comprises a plurality of bacteria of Bacillus velezensis strain ORN14 and / or a functional homolog thereof. According to certain embodiments, the bacterial preparation comprises a plurality of bacteria of Bacillus subtillis strain ORN160 and / or a functional homolog thereof. According to certain currently exemplary embodiments, the bacterial preparation comprises a combination of a plurality of bacteria of Bacillus velezensis strain ORN 14 and / or a functional homolog thereof and a plurality of bacteria of Bacillus subtillis strain ORN160 and / or a functional homolog thereof. According to certain embodiments, the combined preparation is characterized by a synergistic pesticide activity against at least one plant pathogenic nematode pest compared to the activity of a preparation of each bacterial strain or functional homolog thereof alone. According to certain embodiments, the combined preparation is characterized by a synergistic pesticide activity against at least one plant pathogenic fungi, insect, and / or nematode. According to some particular embodiments, the combined preparation is characterized by a synergistic fungicidal and nematocidal activity. In some embodiments, ORN14 and ORN160 strains are more effective against fungal infection, insect, and / or nematode than when the ORN14 and the ORN160 each is applied alone. In some embodiments, ORN14 is more effective against fungal infection, insect, and / or nematode when applied in combination with ORN160 than when the same amount of ORN14 is applied not in combination with the same amount of the ORN160. In some particular embodiments, ORN14 is more effective against fungal infection when applied in combination with ORN160 than when the same amount of ORN14 is applied not in combination with the same amount of the ORN160. In further particular embodiments, ORN14 is more effective against nematode infection when applied in combination with ORN160 than when the same amount of ORN14 is applied not in combination with the same amount of the ORN160. In some embodiments, ORN160 is more effective against fungal infection, insect, and / or nematode when applied in combination with ORN14 than when the same amount of ORN160 is applied not in combination with the same amount of the ORN14. In some particular embodiments, ORN160 is more effective against nematode infection when applied in combination with ORN14 than when the same amount of ORN160 is applied not in combination with the same amount of the ORN14. In further particular embodiments, ORN160 is more effective against fungal infection when applied in combination with ORN14 than when the same amount of ORN160 is applied not in combination with the same amount of the ORN14. In some embodiments, ORN14 improves the fungicidal, nematocidal and / or insecticidal efficacy of the ORN160 compared to when the same amount of ORN160 is applied not in combination with the ORN14. In some embodiments, fungicidal, nematocidal and / or insecticidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, 50%, 100%, 200% or 300% compared to when the same amount of ORN160 is applied alone. In some particular embodiments, fungicidal, nematocidal and / or insecticidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of ORN160 is applied alone. Each possibility represents a separate embodiment. In some embodiments, fungicidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, 50%, 100%, 200% or 300% compared to when the same amount of ORN160 is applied alone. In some particular embodiments, fungicidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of ORN160 is applied alone. Each possibility represents a separate embodiment. In some embodiments, nematocidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, 50%, 100%, 200% or 300% compared to when the same amount of ORN160 is applied alone. In some particular embodiments, nematocidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of ORN160 is applied alone. Each possibility represents a separate embodiment. In some embodiments, ORN160 improves the fungicidal, nematocidal and / or insecticidal efficacy of the ORN14 compared to when the same amount of ORN14 is applied not in combination with the ORN160. In some embodiments, fungicidal, nematocidal and / or insecticidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, 50%, 100%, 200%, or 300% compared to when the same amount of ORN14 is applied alone. In some particular embodiments, fungicidal, nematocidal and / or insecticidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of ORN14 is applied alone. Each possibility represents a separate embodiment. In some embodiments, fungicidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, 50%, 100%, 200% or 300% compared to when the same amount of ORN14 is applied alone. In some particular embodiments, fungicidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of ORN14 is applied alone. Each possibility represents a separate embodiment. In some embodiments, nematocidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, 50%, 100%, 200%, or 300% compared to when the same amount of ORN14 is applied alone. In some particular embodiments, nematocidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of ORN14 is applied alone. Each possibility represents a separate embodiment. In some embodiments, fungicidal, nematocidal and / or insecticidal efficacy is measured at least 7 days after treatment. In some embodiments, fungicidal, nematocidal and / or insecticidal efficacy is measured at least 14 days after treatment. In some embodiments, fungicidal, nematocidal and / or insecticidal efficacy is measured at least 21 days after treatment. In some embodiments, fungicidal, nematocidal and / or insecticidal efficacy is measured at least 28 days after treatment. In some embodiments, a substantially similar level of fungicidal, nematocidal and / or insecticidal efficacy is achieved by using a lesser amount of the ORN14 and / or the ORN160 strains compared to when the ORN14 and the ORN160 each is applied alone. In some embodiments, the combination comprises the combination of ORN14, ORN 160 and one or more additional active agents, wherein the combination is more effective against fungal infection, insect, and / or nematode compared to the effect of each of the ORN 14, ORN 160 and the one or more additional active agents when applied alone at the same amount. According to certain embodiments, the active agent is an additional pesticidal agent. According to certain exemplary embodiments, the additional pesticidal agent is a chemical pesticide. According to some non-limiting exemplary embodiments, the chemical pesticide may include at least one of fluensulfone, fluazinam, chlorantraniliprole, and polyphenol. According to further exemplary embodiments, the additional pesticidal agent is a biological pesticide. According to some particular embodiments the additional biological pesticide may include at least one of plant extract, algae extract, and a combination thereof. According to certain embodiments, the bacterial preparation comprises a culture medium. Culture media suitable for the growth of Bacillus strains, and in particular of Bacillus velezensis and Bacillus subtillis according to the teachings of the present invention are known to a person skilled in the Art. According to certain embodiments, the plurality of bacteria of the at least one bacterial strain or functional homolog thereof is present in the preparation at a concentration which exceeds that found in nature. According to certain embodiments, the bacterial preparation comprises viable cells. According to some embodiments, the viable cells are in a dormant form (endospore). According to additional or alternative embodiments, the bacterial preparation comprises non-viable cells. According to certain additional aspects, the present invention provides a lysate of at least one cell of Bacillus velezensis strain ORN14 and / or a functional homolog thereof; a lysate of at least one cell of Bacillus subtillis strain ORN160 and / or a functional homolog thereof; or a lysate of a combination thereof. According to some embodiments, the lysate comprises a whole cell lysate of the bacterial cells. According to some embodiments, the lysate comprises a soluble fraction of the bacterial cells. According to some embodiments, the lysate comprises inclusion bodies of a bacterial preparation. According to certain further aspects, the present invention provides a cell extract of Bacillus velezensis strain ORN 14 and / or a functional homolog thereof; a cell extract of Bacillus subtillis strain ORN160 and / or a functional homolog thereof; or a cell extract of a combination thereof. According to yet certain additional aspects, the present invention provides a whole cell broth collected from fermentation of at least one cell of Bacillus velezensis strain ORN14 and / or a functional homolog thereof; of at least one cell of Bacillus subtillis strain ORN 160 and / or a functional homolog thereof; or from a fermentation of a combination of at least one cell of Bacillus velezensis strain ORN 14 and / or a functional homolog thereof and of at least one cell of Bacillus subtillis strain ORN160 and / or a functional homolog thereof. Without wishing to be bound by any specific theory or mechanism of action, the fungicidal, nematocidal and / or insecticidal activity of the broth is attributed to secondary metabolites produced by the Bacillus strains of the invention. According to certain embodiments, the lysate, extract or broth is obtained from a plurality of cells of each of the bacterial strain or the combination thereof. The present invention now discloses that combining the strains of the invention, each alone or a combination thereof with a seaweed extract, particularly extract of brown algae, results in a significantly improved pesticidal activity compared to the activity of the strains without the seaweed extract. In some embodiments, the improved activity is synergistic. Thus, according to yet additional aspect, the present invention provides a combination of (a) a plurality of bacteria of at least one bacterial strain selected from the group consisting of Bacillus velezensis strain 0RN14 and / or a functional homolog thereof and Bacillus subtillis strain ORN160 and / or a functional homolog thereof; and (b) a seaweed extract. According to certain embodiments, the weight ratio between the seaweed extract and the plurality of bacteria as described in (a) is between 10:1 to 1:100. According to some particular embodiments, the weight ratio is from about 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, or 1:1 to about 1:2, 1:5, 1:10, 1:15, 1:20, 1:30, or 1:50. According to further specific embodiments the weight ratio is from about 3:1, 2:1, or 1:1, to about 1:2, 1:3, 1:4 or 1:5. Each range or sub-range represents a separate embodiment of the invention. According to some embodiments the weight ratio between the seaweed extract and the fermentation products of the bacterial strains as describe in (a) is between 10:1 to 1:100. According to some particular embodiments, the molar ratio is from about 9:1,8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, or 1:1 to about 1:2, 1:5, 1:10, 1:15, 1:20, or 1:50. According to further specific embodiments the weight ratio is from about 3:1, 2:1, or 1:1, to about 1:2, 1:3, 1:4 or 1:5. Each range or sub-range represents a separate embodiment of the invention. According to further specific embodiments the weight ratio is 1:1. According to some embodiments, the combination of the seaweed extract with the plurality of bacteria as described in (a), or with the products thereof, shows a synergistic effect. According to certain embodiments, the combination is characterized by a synergistic pesticide activity against at least one plant pathogenic pest compared to the activity the plurality of bacteria as described in (a) and / or of the seaweed extract alone. In some embodiments, the seaweed extract improves the pesticidal efficacy of the plurality of bacteria as described in (a) compared to when the same amount of plurality of bacteria as described in (a) is applied not in combination with the seaweed extract. In some embodiments, pesticidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, 50% 100%, 200%, or 300% compared to when the same amount of plurality of bacteria as described in (a) is applied alone. According to some particular embodiments, pesticidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of plurality of bacteria as described in (a) is applied alone. Each possibility represents a separate embodiment of the invention. In some embodiments, plurality of bacteria as described in (a) improves the pesticidal efficacy of the seaweed extract compared to when the same amount of the seaweed extract is applied not in combination with the plurality of bacteria as described in (a). In some embodiments, pesticidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, 50%, 100%, 200%, or 300% compared to when the same amount of the seaweed extract is applied alone. According to some particular embodiments, pesticidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of seaweed extract is applied alone. Each possibility represents a separate embodiment of the invention. According to certain embodiments, the seaweed is of a brown alga of a family selected from the group consisting of Fucaceae, Laminariaceae, Durvillaeaceae and Lessoniaceae. According to certain exemplary embodiments, the Fucaceae seaweed is Ascophyllum nodosum. According to certain exemplary embodiments, the Laminariaceae seaweed is selected from the group consisting of Laminaria hyperborean', Laminaria japonica', Laminaria digitata, Macrocystis pyrifera and Laminaria maxima. Each possibility represents a separate embodiment of the present invention. According to certain exemplary embodiments, the Durvillaeaceae seaweed is a Durvillaea species. According to further certain exemplary embodiments, the Lessoniaceae seaweed is a Lessonia species. The bacterial strains and homologs thereof are as described hereinabove. According to certain embodiments, the seaweed extract is water-soluble algae concentrate obtained by alkaline hydrolysis using potassium hydroxide. According to some particular embodiments, an additional active agent, as described hereinbelow, is added to the combination of the seaweed extract with the plurality of bacteria as described in (a), or with the products thereof. According to some particular embodiments, the additional active agent is polyphenol. According to further certain aspects, the present invention provides an agricultural composition comprising a plurality of bacteria of at least one bacterial strain selected from the group consisting of Bacillus velezensis strain ORN14 and / or a functional homolog thereof; Bacillus subtillis strain ORN160 and / or a functional homolog thereof; and a combination thereof; a preparation of same, a lysate, a broth or an extract thereof. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the agricultural composition further comprises at least one seaweed extract. According to certain currently exemplary embodiments, the agricultural composition is a pesticidal composition further comprising an agriculturally acceptable diluent(s) or carrier(s). According to certain embodiments, the agricultural / pesticidal composition further comprises at least one additional active agent selected from the group consisting of a fertilizer, an acaricide, a fungicide, an additional insecticide, an additional nematicide, a pesticide, a plant growth regulator, a rodenticide, a nutrient and any combination thereof. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the at least one additional active agent is a synthetic agent. According to certain currently exemplary embodiments, the agricultural composition is nematocidal composition comprising at least one additional nematicide. According to certain embodiments, the additional nematicide is selected from the group consisting of fluensulfone, abamectin, thiodicarb, benfuracarb, fluopiram, garlic extract, carbosulfan, caduzafos, terbufos, Pochonia, Pasteuria, Paecilomyces and Azadiractine. Each possibility represents a separate embodiment of the present invention. According to certain exemplary embodiments, the nematicide is fluensulfone. According to some embodiments, fluensulfone is present in the agricultural composition at a w / w concentration of between about 10% to about 50%, including each value within the specified range. According to some particular embodiments, fluensulfone is present in the agricultural composition at a w / w concentration of between about 15% to about 45%, about 15% to about 35%, about 25% to about 45%, about 20% to about 45%, or about 25% to about 38%, including each value within the specified ranges. Each possibility represents a separate embodiment of the invention. According to some embodiments, the amount of fluensulfone in the applied agricultural composition is between 250 to 5,000 g a.i. / ha. According to some particular embodiments the amount of fluensulfone is between 250-2,000, between 250-1,000, between 500-2,000, between 500-1,000, between 1,000-5,000, between 1,000-4,000, or between 1,500-3,000. According to some specific embodiments the amount of fluensulfone in the agricultural composition is between 500 to 3000 g(ai) / ha, including each value within the specified range. Each possibility represents a separate embodiment of the invention. According to additional certain currently exemplary embodiments, the agricultural composition is fungicidal composition comprising at least one additional fungicide. According to certain embodiments, the additional fungicide is selected from the group consisting of copper fungicides (including acypetacs-copper, Bordeaux mixture, Burgundy mixture, Cheshunt mixture, copper acetate, basic copper carbonate, copper hydroxide, copper naphthenate, copper oleate, copper oxychloride, copper silicate, copper sulfate, basic copper sulfate, copper zinc chromate, cufraneb, cuprobam, cuprous oxide, mancopper, oxine-copper, saisentong, thiodiazole-copper); morpholin group (dimethomorph, fenpropidin, fenpropimorph); fosetyl-Al; DMI group (tetraconazole, tebuconazole, cyproconazole, difenoconazole; flutriafol); propineb; Q0I (Strobilurin) group (picoxystrobin, pyraclostrobin, azoxystrobin); Benzimidazol group (captan, carbendazim, tiophanat methyl, thiabendazol); succinate dehydrogenase inhibitor (SDHI) group; fluazinam; thira;, mancozeb; chlorotalonyl; potassium phosphite; mefenoxam; benlaxyl; matalaxyl; and folpet. Each possibility represents a separate embodiment of the present invention. According to some specific embodiments, the fungicide is fluazinam. According to some embodiments, fluazinam is present in the agricultural composition at a w / w concentration of between about 10% to about 50%, including each value within the specified range. According to some particular embodiments, fluazinam is present in the agricultural composition at a w / w concentration of between about 15% to about 45%, about 15% to about 35%, about 25% to about 45%, about 20% to about 45%, or about 25% to about 38%, including each value within the specified ranges. Each possibility represents a separate embodiment of the invention. According to some embodiments, the amount of fluazinam in the agricultural composition, when the agricultural composition is intended for foliar spray or in furrow application, is between 50 to 1,000 g a.i. / ha, including each value within the specified range. According to some particular embodiments the amount of fluazinam is between 50-500, between 50-200, between 100-1,000, between 100-750, between 100-500, or between 250-1,000 g a.i. / ha. Each possibility represents a separate embodiment of the invention. According to some specific embodiments the amount of fluazinam in the agricultural composition is between 250 and 500 g a.i. / ha, including each value within the specified range. According to some specific embodiments, the amount of fluazinam in the agricultural composition is selected from the group consisting of: about 250 g a.i. / ha, about 375 g a.i. / ha, and about 500 g a.i. / ha. Each possibility represents a separate embodiment. According to some embodiments, the amount of fluazinam in the agricultural composition, when the agricultural composition is intended for seed treatment, is between 1 to 100 g a.i. / Kg of seeds. According to some particular embodiments the amount of fluazinam is between 1-50, between 1-20, between 2-50, between 2-20, between 5-75, or between 5-50 g a.i. / lOO Kg of seeds. Each possibility represents a separate embodiment of the invention. According to some specific embodiments the amount of fluazinam in the agricultural composition is between 5 to 30 g a.i. / lOO Kg of seeds, including each value within the specified range. According to yet further certain currently exemplary embodiments, the agricultural composition is insecticidal composition comprising at least one additional insecticide. According to certain embodiments, the additional insecticide is selected from the group consisting of fipronil, chlorantraniliprole, metaflumizone, bifenthrin, imidacloprid, thiametoxam, clothinidin, thiodicarb, thiacloprid, terbuphos, caduzaphos, ethiprole, dinotefuran, polyphenol, and benfuracarb. Each possibility represents a separate embodiment of the present invention. According to some particular embodiments, the additional insecticide is polyphenol. According to some particular embodiments, the additional insecticide is chlorantraniliprole. According to some embodiments, chlorantraniliprole is present in the agricultural composition at a w / w concentration of between about 10% to about 50%, including each value within the specified range. According to some particular embodiments, chlorantraniliprole is present in the agricultural composition at a w / w concentration of between about 15% to about 45%, about 15% to about 35%, about 25% to about 45 %, about 20% to about 45 %, or about 25 % to about 38%, including each value within the specified ranges. Each possibility represents a separate embodiment of the invention. According to some embodiments, the amount of chlorantraniliprole in the agricultural composition, when the agricultural composition is intended for seed treatment, is between 10 to 500 g(ai) / Kg of seeds. According to some particular embodiments the amount of chlorantraniliprole is between 10-100, between 10-50, between 25-500, between 25-250, between 50-500, or between 100-500 g(ai) / 100 Kg of seeds. Each possibility represents a separate embodiment of the invention. According to some specific embodiments the amount of chlorantraniliprole in the agricultural composition is between 30 to 250 g(ai) / 100 Kg of seeds, including each value within the specified range. The agricultural composition can be formulated in any form suitable for applying the composition to a plant or a part thereof or to the plant habitat as is known in the art. According to certain embodiments, the agricultural composition is formulated in a form selected from the group consisting of a liquid, a solid, a semi-solid, a gel, a pressurized and a pressurizable form. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the formulation is in a form selected from the group consisting of wettable powders (WP), emulsion concentrates (EC), microemulsion concentrates (MEC), water-soluble powders (SP), water-soluble concentrates (SL), suspoemulsion (SE), oil dispersions (OD), concentrated emulsions (BW) such as oil-in-water and water-in-oil emulsions, sprayable solutions or emulsions, micro-emulsions (ME), capsule suspensions (CS), suspension concentrates (SC), dusts (DP), oil-miscible solutions (OL), seed-dressing products, granules (GR) in the form of microgranules, spray granules, coated granules and absorption granules, granules for soil application or broadcasting, water-soluble granules (SG), water-dispersible granules (WDG), ULV formulations, microcapsules, and waxes. Each possibility represents a separate embodiment of the present invention. These individual formulation types are known in the art. According to certain embodiments, the formulation is substantially stable for at least 30 days at a temperature range of from about 4°C to about 37°C. According to certain exemplary embodiments, the formulation is substantially stable at a temperature range of from about 20°C to 25°C for more than 30 days. According to certain additional exemplary embodiments, the formulation is substantially stable at a temperature range of from about 20°C to 25°C for at least 6 months, at least 12 months, at least 18 months or about 24 months when in a form of SC, WDG or WP. According to certain embodiments, the formulation further comprises at least one additive selected from the group consisting of a stabilizer, a tackifier, a preservative, a carrier, a surfactant, and a combination thereof. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the carrier is a plant seed. According to these embodiments, the present invention provides a pesticidal composition comprising at least one plant seed and at least one bacterial strain selected from the group consisting of Bacillus velezensis strain ORN14 and / or a functional homolog thereof; Bacillus subtillis strain ORN160 and / or a functional homolog thereof; and a combination thereof, a preparation of same, a lysate, a broth or an extract thereof. Each possibility represents a separate embodiment of the present invention. According to some embodiments, the pesticidal composition further comprises an extract of at least one seaweed. The seaweed extract is as described hereinabove. According to certain currently exemplary embodiments, the pesticidal composition is in a form of seed coating. According to these embodiments, the seed coating formulation further comprises at least one agent selected from the group consisting of a binding agent and a wetting agent. According to certain exemplary embodiments, the binding agent is carboxymethyl cellulose (CMC). According to yet additional certain aspects, the present invention provides a seed coated with the pesticidal composition comprising at least one bacterial strain, a lysate, an extract or a broth thereof, or a combination thereof with at least one active pesticidal agent, as described herein. According to some embodiments, the pesticidal composition further comprises an extract of at least one seaweed. According to yet further aspects, the present invention provides a combination of (a) a plurality of bacteria of at least one bacterial strain selected from the group consisting of Bacillus velezensis strain 0RN14 and / or a functional homolog thereof and Bacillus subtillis strain ORN160 and / or a functional homolog thereof; and (b) at least one additional active agent selected from a nematicide, an insecticide and a fungicide. The nematicide, insecticide and fungicide are as described hereinabove. According to some particular embodiments, the amount of the known additional active agent, in the combination, is lower than the amount of active agent known to be used / effective (herein "sub effective dose"), while the pesticidal activity achieved by the combination is at least equal to the activity of the combination when the known / effective dose is used. The known / effective dose is defined as the range of doses as set out in the regulatory-approved and published product labels of manufacturers of these pesticides, suitable for use under agricultural field conditions. The sub effective dose may be between 10% to 75% of the known / effective dose, including each value within the specified range. According to certain embodiments, one or more component of the combination and / or of a composition comprising same is provided separately. According to certain additional or alternative embodiments, the combination is provided in a single composition. According to certain additional or alternative embodiments, the combination is a tank-mix. According to certain additional or alternative embodiments, the combination is synergistic. According to some embodiments, the combination of the plurality of bacteria as described in (a) and the at least one additional active agent as described in (b) is more effective in inhibiting the growth and / or the development and / or the activity of, or in killing, at least one plant nematode and / or plant pathogenic fungus and / or insect plant pest compared to the effect of the least one additional active agent when applied alone. According to some embodiments, the combination of the plurality of bacteria as described in (a) and the at least one additional active agent as described in (b) is more effective in inhibiting the growth and / or the development and / or the activity of, or in killing, at least one plant nematode and / or plant pathogenic fungus and / or insect plant pest compared to each of the bacteria and at the least one additional active agent applied alone. According to some embodiments, the combination of the plurality of bacteria and of the at least one agent provides higher efficacy compared to the efficacy of each of the plurality of bacteria and at least one agent when each applied alone, when the amount of each component in the combination is the same or lower compared to the amount of said component amount when applied alone. According to some embodiments, the combination of the plurality of bacteria and of the at least one agent provides same efficacy compared to the efficacy of each of the plurality of bacteria and at least one agent when each applied alone, when the amount of each component in the combination is lower compared to the amount of said component amount when applied alone. According to some embodiments, the nematocidal and / or fungicidal and / or insecticidal efficacy of the combination is at least 5%, 10%, 20%, or 30% higher compared to the efficacy of the at least one additional active agent when applied alone. According to further certain embodiments, the nematocidal and / or fungicidal and / or insecticidal efficacy of the combination is at least 40%, 50%, 100%, 200% or 300% compared to the efficacy of the at least one additional active agent when applied alone. According to certain exemplary embodiments, the amount of the at least one additional active agent in the combination is the same amount of said at least one additional active agent when applied alone. According to certain embodiments, the additional fungicide in the combination is fluazinam. According to certain embodiments, the weight ratio between fluazinam and the plurality of bacteria as described in (a) is between 20:1 to 1:100. According to some particular embodiments, the weight ratio is from about 15: 1, 12:1, 10:1 9:1, 8:1,7:1, 6:1, 5:1, 4:1, 3:1, 2:1, or 1:1 to about 1:2, 1:5, 1:10, 1:15, 1:20, 1:30, or 1:50. According to further specific embodiments the weight ratio is from about 3:1, 2:1, or 1:1, to about 1:2, 1:3, 1:4 or 1:5. Each range or sub-range represents a separate embodiment of the invention. According to further particular embodiments the weight ratio between fluazinam and the plurality of bacteria as described in (a) is about 1:1. According to some embodiments the weight ratio between fluazinam and the fermentation products of the bacterial strains as describe in (a) is between 20:1 to 1:100. According to some particular embodiments, the molar ratio is from about 15:1,12:1,10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, or 1:1 to about 1:2, 1:3, 1:5, 1:10, 1:15, 1:20, 1:30, or 1:50. According to further specific embodiments the weight ratio is from about 3:1, 2:1, or 1:1, to about 1:2, 1:3, 1:4 or 1:5. Each range or sub-range represents a separate embodiment of the invention. According to some embodiments, the combination with fluazinam provides both nematocidal and fungicidal effect. According to some embodiments, the combination of ORN14 with fluazinam provides both nematocidal and fungicidal effect. According to some embodiments, the combination of ORN160 with fluazinam provides nematocidal and fungicidal effect. According to some embodiments, the combination of ORN14, ORN160 and fluazinam provides nematocidal and fungicidal effect. According to certain embodiments, the additional nematicide in the combination is fluensulfone. According to certain embodiments, the weight ratio between fluensulfone and the plurality of bacteria as described in (a) is between 500:1 to 1:10. According to some particular embodiments, the weight ratio is from about 200:1, 100:1, 90:1, 80:1, 70:1, 60:1, 50:1, 40:1, 30:1, 20:1, 10:1, or 5:1 to about 1:2, 1:3, 1:4, 1:5, or 1:10. According to further specific embodiments the weight ratio is about 80:1, 70:1, 60:1, 50:1,40:1, 30:1,20:1, or 10:1. Each range or sub-range represents a separate embodiment of the invention. According to some embodiments the weight ratio between fluensulfone and the fermentation products of the bacterial strains as describe in (a) is between 500:1 to 1:10. According to some particular embodiments, the weight ratio is from about 200:1, 100:1, 90:1, 80:1, 70:1, 60:1, 50:1, 40:1, 30:1, 20:1, 10:1, or 5:1 to about 1:2, 1:3, 1:4, 1:5, or 1:10. According to further specific embodiments the weight ratio is about 80:1, 70:1, 60:1, 50:1, 40:1, 30:1, 20:1, or 10:1. Each range or sub-range represents a separate 21 embodiment of the invention. According to some embodiments, the combination with fluensulfone provides nematocidal or both nematocidal and fungicidal effect. According to some embodiments, the combination of ORN14 with fluensulfone provides both nematocidal and fungicidal effect. According to some embodiments, the combination of ORN160 with fluensulfone provides nematocidal effect. According to some embodiments, the combination of ORN14, ORN160 and fluensulfone provides nematocidal and fungicidal effect. According to some particular embodiments, the additional insecticide in the combination is chlorantraniliprole. According to certain embodiments, the weight ratio between chlorantraniliprole and the plurality of bacteria as described in (a) is between 30:1 to 1:10. According to some particular embodiments, the weight ratio is from about 20:1, 15:1, 12:1, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, or 1:1 to about 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, or 1:9. According to further specific embodiments the weight ratio is from about 8:1, 5:1, 4:1, 3:1, 2:1 or 1:1, to about 1:2, 1:3, 1:5. Each range or sub-range represents a separate embodiment of the invention. According to some embodiments the weight ratio between chlorantraniliprole and the fermentation products of the bacterial strains as describe in (a) is between 30:1 to 1:10. According to some particular embodiments, the weight ratio is from about 20:1, 15:1, 12:1, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1,4:1, 3:1, 2:1, or 1:1 to about 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, or 1:9. According to further specific embodiments the weight ratio is from about 8:1, 5:1, 4:1, 3:1, 2:1 or 1:1, to about 1:2, 1:3, 1:5. Each range or sub-range represents a separate embodiment of the invention. According to some embodiments, the combination with chlorantraniliprole provides at least one of an insecticidal effect, nematocidal effect, fungicidal effect, or combination thereof. According to some embodiments, the combination of ORN14 with chlorantraniliprole provides both nematocidal and insecticidal effect. According to some embodiments, the combination of ORN160 with chlorantraniliprole provides insecticidal and nematocidal effect. According to some embodiments, the combination of ORN160 with ORN14 and chlorantraniliprole provides at least one of an insecticidal, fungicidal, nematocidal effect, or a combination thereof. According to particular embodiments, the combination of fluazinam with the plurality of bacteria as described in (a), or with the products thereof, shows at least an additive effect, and preferably a synergistic effect. According to certain embodiments, the combination is characterized by a enhanced fungicide activity against at least one plant pathogenic fungi compared to the activity the plurality of bacteria as described in (a) and / or of fluazinam alone. According to some embodiments, the plurality of bacteria as described in (a) is ORN14, and / or a functional homolog thereof. In some embodiments, fluazinam improves the fungicidal efficacy of the plurality of bacteria as described in (a) compared to when the same amount of plurality of bacteria as described in (a) is applied not in combination with fluazinam. In some embodiments, fungicidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, 50% or 100% 200% or 300% compared to when the same amount of plurality of bacteria as described in (a) is applied alone. In some particular embodiments, fungicidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of plurality of bacteria as described in (a) is applied alone. Each possibility represents a separate embodiment of the invention. According to some embodiments, the plurality of bacteria as described in (a) is ORN 14, and / or a functional homolog thereof. In some embodiments, plurality of bacteria as described in (a) improves the fungicidal efficacy of fluazinam compared to when the same amount of fluazinam is applied not in combination with the plurality of bacteria as described in (a). In some embodiments, fungicidal efficacy is increased by at least 5%, 10%, 20%, 30%, 50%, 100%, 200% or 300% compared to when the same amount of fluazinam is applied alone. In some particular embodiments, fungicidal efficacy is increased by at least 5%, 10%, 20%, 30% or 50% compared to when the same amount of fluazinam is applied alone. Each possibility represents a separate embodiment of the invention. According to some embodiments, the plurality of bacteria as described in (a) is ORN14, and / or a functional homolog thereof. According to particular embodiments, the combination of fluensulfone with the plurality of bacteria as described in (a), or with the products thereof, shows a synergistic effect. According to certain embodiments, the combination is characterized by an enhanced nematicide activity against at least one plant pathogenic nematode compared to the activity the plurality of bacteria as described in (a) and / or of fluensulfone alone. In some embodiments, fluensulfone improves the nematocidal efficacy of the plurality of bacteria as described in (a) compared to when the same amount of plurality of bacteria as described in (a) is applied not in combination with fluensulfone. In some embodiments, nematocidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, 50%, 100%, 200% or 300% compared to when the same amount of plurality of bacteria as described in (a) is applied alone. In some particular embodiments, nematocidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of plurality of bacteria as described in (a) is applied alone. Each possibility represents a separate embodiment of the invention. According to some embodiments, the plurality of bacteria as described in (a) is ORN14, and / or a functional homolog thereof. In some embodiments, plurality of bacteria as described in (a) improves the nematocidal efficacy of fluensulfone compared to when the same amount of fluensulfone is applied not in combination with the plurality of bacteria as described in (a). In some embodiments, nematocidal efficacy is increased by at least 5%, 10%, 20%, 30%, 50%, 100%, 200% or 300% compared to when the same amount of fluensulfone is applied alone. In some particular embodiments, nematocidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of fluensulfone is applied alone. Each possibility represents a separate embodiment of the invention. According to some embodiments, the plurality of bacteria as described in (a) is ORN14, and / or a functional homolog thereof. According to particular embodiments, the combination of chlorantraniliprole with the plurality of bacteria as described in (a), or with the products thereof, shows at least an additive effect, and optionally a synergistic effect. According to certain embodiments, the combination is characterized by a enhanced insecticide, fungicide, and / or nematicide activity against at least one plant pathogenic pest compared to the activity of the plurality of bacteria as described in (a) and / or of chlorantraniliprole alone. In some embodiments, chlorantraniliprole improves the insecticidal efficacy of the plurality of bacteria as described in (a) compared to when the same amount of plurality of bacteria as described in (a) is applied not in combination with chlorantraniliprole. In some embodiments, insecticidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, 50%, 100%, 200% or 300% compared to when the same amount of plurality of bacteria as described in (a) is applied alone. In some particular embodiments, insecticidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of plurality of bacteria as described in (a) is applied alone. Each possibility represents a separate embodiment of the invention. In some embodiments, plurality of bacteria as described in (a) improves the insecticidal efficacy of chlorantraniliprole compared to when the same amount of chlorantraniliprole is applied not in combination with the plurality of bacteria as described in (a). In some embodiments, insecticidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, 50%, 100%, 200% or 300% compared to when the same amount of chlorantraniliprole is applied alone. In some particular embodiments, insecticidal efficacy is increased by at least 5%, 10%, 20%, 30%, 40%, or 50% compared to when the same amount of chlorantraniliprole is applied alone. Each possibility represents a separate embodiment of the invention. According to certain aspects, the present invention provides a container adapted for a watering system of a plant field, comprising the pesticidal composition comprising at least one bacterial strain, a lysate, an extract or a broth thereof, optionally further comprising at least one seaweed extract and / or at least one additional active agent as described herein. According to certain exemplary embodiments, the watering system is a drip irrigation system. According to certain aspects, the present invention provides a kit comprising (i) a pesticidal composition comprising at least one bacterial strain, a lysate, an extract or a broth thereof, optionally further comprising at least one seaweed extract and / or at least one additional active agent as described herein; optionally (ii) a delivery system for applying the pesticidal composition to a plant or a part thereof or to the plant growth medium and (iii) instructions for using the agricultural composition. According to certain embodiments, the instructions for using the pesticidal composition comprise instructions for the amounts and frequency of applying the pesticidal composition as to reduce or prevent damage caused to the plant by a nematode and / or an insect pest. According to further certain aspects, the present invention provides a method for inhibiting or preventing the growth and / or the development of at least one nematode or insect pest present in or on a plant or a part thereof or a plant habitat, comprising contacting the plant or part thereof or plant habitat with at least one bacterial strain selected from the group consisting of Bacillus velezensis strain 0RN14 and / or a functional homolog thereof; Bacillus subtillis strain ORN160 and / or a functional homolog thereof; and a combination thereof, a preparation of same, a lysate, a broth or an extract obtained therefrom or a composition comprising same. Each possibility represents a separate embodiment of the present invention. According to further certain aspects, the present invention provides a method for inhibiting or preventing the growth and / or the development of at least one nematode and / or insect pest and / or fungi, comprising applying at least one bacterial strain selected from the group consisting of Bacillus velezensis strain 0RN14 and / or a functional homolog thereof; Bacillus subtillis strain ORN160 and / or a functional homolog thereof; and a combination thereof, a preparation of same, a lysate, a broth or an extract obtained therefrom or a composition comprising same to a locus where the growth and / or the development of the nematode and / or the insect pest and / or the fungi is to be inhibited or prevented so as to thereby inhibit or prevent the growth and / or the development of at least one nematode and / or the insect pest and / or the fungi. According to certain embodiments, the locus is turf, golf lawns, home gardens, lawns, and ornamentals. According to yet further certain aspects, the present invention provides a method for protecting a plant or a part thereof from a disease or deleterious symptoms associates with the growth and / or the development of at least one nematode or insect pest in or on the plant or a part thereof or the plant habitat, comprising contacting said plant or part thereof or plant habitat with at least one bacterial strain selected from the group consisting of Bacillus velezensis strain 0RN14 and / or a functional homolog thereof; Bacillus subtillis strain ORN160 and / or a functional homolog thereof; and a combination thereof, a preparation of same, a lysate, a broth or an extract obtained therefrom or a composition comprising same. Each possibility represents a separate embodiment of the present invention. According to certain aspects, the present invention provides a method for enhancing plant growth through biotic and / or abiotic factors in the soil, comprising contacting the plant or part thereof or plant habitat with at least one bacterial strain selected from the group consisting of Bacillus velezensis strain 0RN14 and / or a functional homolog thereof; Bacillus subtillis strain ORN160 and / or a functional homolog thereof; and a combination thereof, a preparation of same, a lysate, a broth or an extract obtained therefrom or a composition comprising same. Each possibility represents a separate embodiment of the present invention. According to to certain aspects, the present invention provides a method for enhancing the root system development which leads to a better water and nutrients extraction from the soil and therefore overall plant health, the method comprising contacting the plant or part thereof or plant habitat with at least one bacterial strain selected from the group consisting of Bacillus velezensis strain 0RN14 and / or a functional homolog thereof; Bacillus subtillis strain ORN160 and / or a functional homolog thereof; and a combination thereof, a preparation of same, a lysate, a broth or an extract obtained therefrom or a composition comprising same. Each possibility represents a separate embodiment of the present invention. Without wishing to be bound by any specific theory or mechanism of action, the fungicidal, nematocidal and / or insecticidal activity of the Bacillus strains of the invention is attributed to secondary metabolites produced by said strains during the establishment of said strain populations in or on the plant or the plant habitat. According to certain embodiments, each of the methods further comprises contacting the plant or part thereof or plant habitat with at least one seaweed extract. According to certain embodiments, the seaweed extract is applied separately. According to these embodiments, the at least one bacterial strain and the seaweed extract can be contacted with the plant or part thereof or the plant habitat concomitantly or sequentially. According to yet additional embodiments, the seaweed extract is applied within the same composition comprising the at least one bacterial strain. According to certain embodiments, the plant or part thereof comprises the at least one nematode and / or fungus and / or insect pest in or on at least one cell or tissue of said plant or part thereof. According to some embodiments, the plant or part thereof comprising the at least one nematode and / or fungus and / or insect pest is affected with symptoms associated with the presence of said at least one nematode and / or insect pest. According to certain currently exemplary embodiments, the method comprises applying a combination of Bacillus velezensis strain ORN14 and / or a functional homolog thereof; Bacillus subtillis strain ORN160 and / or a functional homolog thereof, a lysate, a broth or an extract obtained therefrom or a composition comprising same, thereby obtaining an enhanced (synergistic) inhibitory / protecting effect compared to the inhibitory / protecting effect obtained by applying each strain alone. According to certain embodiment, each of the bacterial strains, microbial preparation, lysate, extract or a whole broth obtained therefrom or a composition comprising same is applied separately. According to certain embodiments, the strains are applied concurrently or sequentially. According to yet alternative embodiments, the combination of bacterial strains, preparation, lysate, broth or an extract obtained therefrom is applied within a single composition. According to yet alternative embodiments, the combination of bacterial strains, preparation, lysate, broth or an extract obtained therefrom each is applied within a separate composition. According to yet further embodiments, the method further comprises contacting the plant, part thereof or plant habitat with at least one additional fungicide, nematicide and / or insecticide. According to certain embodiments, the at least one additional fungicide, nematicide and / or insecticide is applied together with the at least one bacterial strain within the same pesticidal composition. According to certain alternative embodiments, each of the at least one bacterial strain and the at least one additional fungicide, nematicide and / or insecticide is applied separately. According to these embodiments, the application can be contemporaneously or sequentially. In some embodiments, the at least one fungicide is fluazinam. In some embodiments, the at least one nematicide is fluensulfone. In some embodiments, the at least one insecticide is chlorantraniliprole. According to certain embodiments, the plant part is selected from the group consisting of a seed, a root, a shoot, a leaf, a branch, a flower, a fruit and any combination thereof. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the plant is a crop plant. The methods of the present invention may be used with any crop plants, including but not limited to beet, cereals, com, cotton, environmental crops, fruits & nuts, grapes / vines, oilseed-rape / canola, plantation, potatoes, rice, soybeans, vegetables & flowers and other crops. In some embodiments the methods of the present invention may be used with any crop plant, including but not limited to monocotyledons such as sugar cane, cereals, rice, maize (com), and / or; or dicotyledon crop such as beets (such as sugar beet or fodder beet); fmits (such as pomes, stone fmits, or soft fruits, for example apples, pears, plums, peaches, almonds, cherries, strawberries, raspberries, orblackberries); leguminous plants (such as beans, lentils, peas, or soybeans); oil plants (such as rape, mustard, poppy, olives, sunflowers, coconut, castor oil plants, cocoa beans, or groundnuts); cucumber plants (such as marrows, cucumbers or melons); fiber plants (such as cotton, flax, hemp, or jute); citms fmits (such as oranges, lemons, grapefruit, or mandarins); vegetables (such as spinach, lettuce, cabbages, carrots, tomatoes, potatoes, onions, garlics, cucurbits, or paprika); lauraceae (such as avocados, cinnamon, or camphor); tobacco; nuts; coffee; tea; vines; hops; durian; bananas; natural mbber plants; and ornamentals (such as flowers, shmbs, broad-leaved trees, or evergreens, for example conifers). According to certain exemplary embodiments, the crop plants are sugar cane plants. According to yet additional exemplary embodiments, the crop plant is com (maize) plant. According to yet further exemplary embodiments, the crop plant is soybean. According to yet further exemplary embodiments, the crop plant is coffee. According to yet further exemplary embodiments, the crop plant is cotton. According to yet further exemplary embodiments, the crop plant is a fruit. According to yet further exemplary embodiments, the crop plant is a vegetable. Any method as is known in the art for contacting bacterial strains with a plant, a part thereof or the plant habitat can be used according to the teachings of the present invention. Typically, the plant or part thereof is contacted with a composition comprising the bacterial strains, functional homologs thereof or preparations of same. According to some embodiments, the plant can be contacted directly with the composition. According to certain embodiments, the method comprises applying the pesticidal composition of the invention by foliage spraying. According to some embodiments, the composition may be applied to the plant habitat. According to certain embodiments, the plant habitat is a growth medium, which can be a solid or a liquid growth medium. According to certain exemplary embodiments, the solid growth medium is soil. According to these embodiments, the pesticidal composition of the invention is applied to the soil by a method selected from the group consisting of in furrow application, broadcast spray pre-planting incorporation, broadcast spraying on the soil surface, drip irrigation and any combination thereof. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the composition is formulated in a liquid form. According to certain exemplary embodiments, the liquid composition is a SC. According to these embodiments, the plant or part thereof may be contacted with the composition by a method selected from the group consisting of infdtration, immersion / dipping, incubation, spraying (including in furrow spraying), and any combination thereof. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the composition is formulated in a solid form. According to certain exemplary embodiments, the solid for is a dried form, including, but not limited to wettable powder (WP) and water dispersible granules (WDG). According to these embodiments the plant or part thereof may be contacted with the dry composition by a method selected from the group consisting of incubation, dusting, and any combination thereof. Each possibility represents a separate embodiment of the present invention. According to certain exemplary embodiments, the plant part is a seed. According to certain embodiments, the at least one bacterial strain or a composition comprising same according to the teachings of the invention is applied to the seed via seed coating or via administration to the seed habitat. According to some embodiments the seed habitat is soil, and administration is via in furrow application, seed treatment, broadcast spray preplanting incorporation, broadcast spraying on the soil surface, and / or drip irrigation. According to certain additional or alternative exemplary embodiments, the plant part is a leaf. According to some embodiments, the at least one bacterial strain is applied to the leaf by spraying a bacterial preparation or a composition comprising same according to the teachings of the present invention. According to certain embodiments the at least one bacterial strain or a composition comprising same is applied before planting. According to certain embodiments the at least one bacterial strain or a composition comprising same is applied at the time of planting. According to certain embodiments, the nematode is of a genus selected from the group consisting of Heterodera Meloidogyne, Pratylenchus, Xiphinema and Ditylenchus, Helicotylenchus. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the fungus is of a genus selected from the group consisting of Fusarium, Rhizoctonia, Sclerothinia, Macrophomina, Colletotrichum, Phomopsis, Aspergillus, Penicillium, and Cercopora. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the insect pest is of an order selected from the group consisting of Lepidoptera, Coleoptera, Hemiptera. It is to be understood that any combination of each of the aspects and the embodiments disclosed herein is explicitly encompassed within the disclosure of the present invention. Further embodiments and the full scope of applicability of the present invention will become apparent from the detailed description given hereinafter. However, it should be understood that the detailed description and specific examples, while indicating preferred embodiments of the invention, are given by way of illustration only, since various changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description. BRIEF DESCRIPTION OF THE FIGURES FIG. 1 shows the morphology of 0RN14 and ORN160. FIG. 2 shows the phylogenomic tree of 0RN14. FIG. 3 shows the phylogenomic tree of ORN160. DETAILED DESCRIPTION OF THE INVENTION The present invention provides isolated novel Bacillus strains, particularly biologically pure isolated novel strains of Bacillus velezensis and Bacillus subtillis having significant activity against a variety of plant pests, including nematodes, fungi and insects. Particularly, the present invention discloses that a combination of the novel Bacillus strains shows a synergistic activity in controlling the deleterious effects of nematodes and / or plant pathogenic fungi and / or insects on a plant growth, health and typically yield compared to the activity of each of the strains alone. The isolated bacterial strains or their combination can be used in various forms (preparations comprising a plurality of bacterial cells, lysates, extracts, whole cell broth and the like, optionally with an extract of at least one seaweed) as biocontrol agents protecting the plant from the nematode and / or plant pathogeny fungi and / or insect pests, by killing or at least inhibiting the development and / or activity of the pest, or otherwise protecting the plant from a deleterious effect of these pathogens. Definitions The terms “comprise”, “comprising”, “includes”, “including", “having” and their conjugates mean “including but not limited to”. The term “consisting of’ means “including and limited to”. The term “consisting essentially of’ means that the composition, method or structure may include additional ingredients, steps and / or parts, but only if the additional ingredients, steps and / or parts do not materially alter the basic and novel characteristics of the claimed composition, method or structure. As used herein, the singular form “a”, “an” and “the” include plural references unless the context clearly dictates otherwise. For example, the term "a compound" or "at least one compound" may include a plurality of compounds, including mixtures thereof. Throughout this application, various embodiments of this invention may be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible sub-ranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed sub-ranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers (whole or fractional numbers) within that range, for example, 1, 3, 6, 1.5, 3.7, and the like. This applies regardless of the breadth of the range. As used herein, the term “method” refers to manners, means, techniques and procedures for accomplishing a given task, including, but not limited to, those manners, means, techniques and procedures either known to, or readily developed from known manners, means, techniques and procedures by practitioners of the agricultural, chemical, pharmacological, biological, biochemical and medical arts, particularly of the agricultural and chemical arts. When reference is made to particular sequence, such reference is to be understood to also encompass sequences that substantially correspond to its complementary sequence, and as including minor sequence variations, resulting from, e.g., sequencing errors, cloning errors, or other alterations resulting in base substitution, base deletion or base addition, provided that the frequency of such variations is less than 1 in 50 nucleotides, alternatively, less than 1 in 100 nucleotides, alternatively, less than 1 in 200 nucleotides, alternatively, less than 1 in 500 nucleotides, alternatively, less than 1 in 1000 nucleotides, alternatively, less than 1 in 5,000 nucleotides, alternatively, less than 1 in 10,000 nucleotides. As used herein, the terms “sequence identity” or “identity” or grammatical equivalents, in the context of two nucleic acid or polypeptide sequences, includes reference to the residues in the two sequences which are the same when aligned. When percentage of sequence identity is used in reference to proteins, it is recognized that residue positions which are not identical often differ by conservative amino acid substitutions, where amino acid residues are substituted for other amino acid residues with similar chemical properties (e.g., charge or hydrophobicity), and therefore do not change the functional properties of the molecule. Where sequences differ in conservative substitutions, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution. Sequences which differ by such conservative substitutions are considered to have “sequence similarity” or “similarity”. Means for making this adjustment are well-known to those of skill in the art. Typically this involves scoring a conservative substitution as a partial rather than a full mismatch, thereby increasing the percentage sequence identity. Thus, for example, where an identical amino acid is given a score of 1 and a non-conservative substitution is given a score of zero, a conservative substitution is given a score between zero and 1. The scoring of conservative substitutions is calculated, e.g., according to the algorithm of Henikoff S and Henikoff JG (Amino acid substitution matrices from protein blocks. Proc. Natl. Acad. Sci. U.S.A. 1992, 89(22): 10915-9). Identity can be determined using any homology comparison software, including for example, the BlastN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters. According to some embodiments of the invention, the identity is a global identity, i.e., an identity over the entire amino acid or nucleic acid sequences of the invention and not over portions thereof. According to some embodiments, the identity is partial identity, i.e., an identity over certain percentages of the length of the amino acid or nucleic acid sequences as described herein. As used herein, the term "query coverage” refers to a percentage that describes how much of the query sequence is included in the region aligned to the target sequence. As used herein, the terms “marker”, “genomic marker” and “sub-genomic sequence” are used herein interchangeably and refer to a DNA (deoxyribonucleic acid) sequence present within the genome of a microbial strain. According to certain exemplary embodiments, identity of a genomic marker sequence is defined as at least 90% query coverage with at least 95% identity, such as further described herein. The terms “microbial strain(s)” and “bacterial strain(s)” are used herein interchangeably and refer to the bacterial strains of the invention as defined herein. The microbial strains of the present invention are deposited with the American Type Culture Collection (ATCC). Bacterial strains of Accession Numbers PTA-127478 and PTA-127479 (ORN160 and ORN14, respectively), were deposited at American Type Culture Collection, Patent Depository, 10801 University Boulevard, Manassas, Virginia 20110-2209, USA, on October 16, 2023. The terms “functional homolog”, “functionally homologous”, “variant” and grammatical equivalents are used herein interchangeably and refer to a modification (i.e., mutant, at least one mutation) of the bacterial Bacillus strains of the invention resulting in a strain that is endowed with substantially the same ensemble of biological activities, particularly the capability to kill or inhibit the development and / or activity of at least one type of nematode and / or insect pets, or the ability to protect plants from a deleterious effect of the at least pest, (+ / - 10%, 20%, 40%, 50%, or 60% when tested under the same conditions) as that of the Bacillus strain of the invention and can be classified to the same species or strain based on known methods of species / strain classifications and as described herein. The modification can be man-made or evolutionary, e.g., during propagation with or without selection. As used herein, the unit “g a.i.” refers to the weight, measured in grams, of an active ingredient, e.g., a pesticidal agent. As used herein, the unit “ha” refers to a hectare, i.e., 10,000 m2. Thus, the unit “g a.i. / ha”, as is known in the art of agriculture, refers to the weight of the active agent used per hectare of treated land. The terms "synergy", "synergistic effect" and "synergistic activity" are used herein interchangeably and refer to an interaction or cooperation of compounds giving rise to a whole (combination of the compounds) that is more than a simple sum of its parts (the components). According to certain embodiments, the synergistic effect can be quantified according to Colby's formula (i.e. (E)=X+Y-(X*Y / 100); Colby, R. S., 1967. Weeds, 15, 20-22). Thus, by "synergistic" is intended a component which, by virtue of its presence, increases the desired effect by more than an additive amount. Additionally or alternatively to the actual synergistic action with respect to pesticidal activity, the combinations according to the invention also can have surprising advantageous properties which can be described, in a wider sense, as synergistic activity. Examples of such advantageous properties that may be mentioned are: advantageous behavior during formulation and / or upon application, for example upon grinding, sieving, emulsifying, dissolving or dispensing; increased storage stability; improved stability to light; more advantageous degradability; improved toxicological and / or ecotoxicological behavior; or any other advantages familiar to a person skilled in the art. As used herein, the term “tank mix” means one or more of the components of the combination, mixture or composition of the present invention are mixed in a spray tank at the time of spray application or prior to spray application. In certain cases (particularly in aerial application by an airplane, but also by tractor) it is common to use a pre-mixer before loading in the spray tank (inside the airplane or on the tractor). The mixture resulting from use of the pre-mixer is referred to herein as “pre-mix”. As used herein, the term “combination” means an assemblage of active agents / components for application either by simultaneous or contemporaneous application. As used herein, the term “simultaneous” when used in connection with application of active agents / components means that the actives are applied in an admixture, for example, a tank mix. For simultaneous application, the combination may be the admixture or separate containers each containing an active that are combined prior to application. As used herein, the term “contemporaneous” when used in connection with application of actives means that an individual active is applied separately from another active or premixture at the same time or at times sufficiently close together that an activity that is additive or more than additive or synergistic relative to the activity of either agrochemical alone at the same dose is achieved. According to some embodiments, a combination according to the present invention, optionally with one or more customary formulation auxiliaries, is provided in the form of a tank-mix composition. According to further embodiments, a combination according to the present invention, optionally with one or more customary formulation auxiliaries, is provided in the form of a pre-mix composition. Some non-limiting examples of formulation types for pre-mix compositions are GR, WP, WDG, SG, SL, EC, BW, ME, SC, CS, OD, and SE. According so some embodiments the pre-mix compositions are particularly suited for seed treatment application. Some non-limiting examples of seed-dressing products for pre-mix compositions are wettable powders for seed treatment slurry (WS), solution for seed treatment (LS), emulsions for seed treatment (ES), suspension concentrate for seed treatment (FS), WDG, and CS. Some non-limiting examples of formulation types suitable for tank-mix compositions are solutions, dilute emulsions, suspensions, or a mixture thereof, and dusts. The methods of application of the formulation, such as foliar, drench, seed treatment, spraying (such as in furrow spray and soil spray), atomizing, dusting, scattering, coating, pouring, and the like, are chosen in accordance with the intended objectives and the prevailing circumstances. According to some embodiments, the tank-mix compositions are prepared by diluting with a solvent (for example, water) the one or more pre-mix compositions containing different pesticides, and optionally further auxiliaries. Whereas commercial products will preferably be formulated as concentrates (e.g., pre-mix composition (formulation)), the end user will normally employ dilute formulations (e.g., tank mix composition). However, tank mix compositions may be provided commercially, and pre-mix compositions may be used by an end user. The terms “isolated” and “biologically pure” with reference the Bacillus strains of the invention relates to bacterium or bacteria of the same species at least partially separated from the natural environment e.g., from the microbial strain habitat or from one or more constituents thereof, cellular or otherwise, with which it may be associated if found in nature. According to certain embodiments, bacterial strains can be isolated from the plant (the strain being part of the plant microbiome), from the plant habitat (including the plant rhizoplane and rhizosphere), or from the insect (the insect microbiome). The bacterial strains of the invention were isolated from the soil at the Applicant (ADAMA) Brazil site located at Latitude: -23.2765 Longitude: -51.1137. As used herein, the term “rhizoplane” refers to the external surface of roots together with closely adhering soil particles and debris. As used herein, the term “rhizosphere” refers to the region of soil in the vicinity of plant roots, in which the soil chemistry and microbiology is influenced by the plant root growth, respiration, and nutrient exchange. As used herein, the term “phyllosphere” refers to the total above-ground portions of a plant, which are a habitat for microorganisms. The phyllosphere can be further subdivided into the caulosphere (stems), phylloplane (leaves), anthosphere (flowers), and carposphere (fruit). The terms “whole cell broth” and “whole cell culture” are used herein interchangeably and refer to a liquid culture containing both cells and a liquid medium. In particular embodiments, the terms relate to liquid medium comprising at least one bacterial strain of the invention and fermentation products thereof. According to certain aspects, the present invention provides an isolated bacterial strain or a functional homolog thereof, wherein the isolated bacterial strain is selected from the group consisting of Bacillus velezensis strain 0RN14; and Bacillus subtillis strain ORN160. According to certain embodiments, Bacillus velezensis strain 0RN14 is selected from the group consisting of (a) a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO: 1; (b) a strain deposited under ATCC Accession Number PTA-127479; and a combination thereof. According to certain embodiments, Bacillus velezensis strain 0RN14 is selected from the group consisting of (a) a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:3; (b) a strain deposited under ATCC Accession Number PTA-127479; and a combination thereof. According to certain embodiments, Bacillus subtillis strain ORN160 is selected from the group consisting of (a) a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:2; (b) a strain deposited under ATCC Accession Number PTA-127478; and a combination thereof. According to certain embodiments, Bacillus subtillis strain ORN160 is selected from the group consisting of (a) a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:4; (b) a strain deposited under ATCC Accession Number PTA-127478; and a combination thereof. According to certain aspects, the present invention provides a microbial combination comprising (i) Bacillus velezensis strain ORN14 and / or functional homolog thereof and (ii) Bacillus subtillis strain ORN160 and / or functional homolog thereof, wherein the combination has an enhanced capability to inhibit the growth and / or the development and / or the activity of, or to kill, at least one plant nematode and / or insect pest compared to the capability of each strain alone. According to certain embodiments, the combination shows synergistic activity. According to certain embodiments, the combination comprises the Bacillus velezensis strain ORN14 and the Bacillus subtillis strain ORN160 at a ratio of from about 10:1 to about 1:10, or a ratio of from about 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, or 2:1 to about 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, or 1:2. According to certain exemplary embodiments, the combination comprises the Bacillus velezensis strain ORN14 and the Bacillus subtillis strain ORN160 at a ratio of 1:1. It is to be explicitly understood that the present invention encompasses a plurality of the at least one bacterial strains or functional homologs of the invention, complete cultures comprising a growth medium and at least one of the bacterial strains or functional homologs of the invention, as well as growth medium obtained after removal of the bacterial strains. According to certain aspects, the present invention provides a whole cell broth collected from fermentation of at least one cell of Bacillus velezensis strain ORN 14 and / or a functional homolog thereof; of at least one cell of Bacillus subtillis strain ORN 160 and / or a functional homolog thereof; or from a fermentation of a combination of at least one cell of Bacillus velezensis strain ORN 14 and / or a functional homolog thereof and of at least one cell of Bacillus subtillis strain ORN160 and / or a functional homolog thereof. According to the certain embodiments, the cell broth is of a plurality of bacteria of a single strain. According to some embodiments, the cell broth is of a plurality of strains. According to a further aspect, the present invention provides a lysate of at least one bacterial strain or of a functional homolog thereof according to the teachings of the present invention. According to the certain embodiments, the lysate is of a single strain. According to some embodiments, the lysate is of a plurality of strains. According to certain embodiments, the lysate comprises a whole cell lysate of the bacteria. According to certain embodiments, the lysate comprises a soluble fraction of the bacterial cells. According to a further aspect, the present invention provides a cell extract of at least one bacterial strain or a functional homolog thereof according to the teachings of the present invention. According to some embodiments, the cell extract is of a plurality of strains. According to some embodiments, the bacterial preparation, culture, cell broth, lysate and / or extract are included in an agricultural composition. According to some embodiments the agricultural composition further comprises agriculturally acceptable carriers and / or diluents. According to additional embodiments, the composition further comprises an agriculturally effective amount of at least one additional active agent selected from the group comprising a fertilizer, an acaricide, a fungicide, an insecticide, a nematicide, a plant growth regulator, a rodenticide, and a nutrient. According to some embodiments of the present invention, a plurality of bacteria of the strains of the present invention and / or functional homologs thereof, each alone or a combination thereof, are combined with an extract of at least one seaweed of a family selected from the group consisting of Fucaceae, Laminariaceae, Durvillaeaceae and Lessoniaceae. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the seaweed extract is water-soluble algae concentrate obtained by alkaline hydrolysis using potassium hydroxide. According to certain exemplary embodiments, the Fucaceae seaweed is Acophyllus nodosum. According to certain exemplary embodiments, the Laminariaceae seaweed is selected from the group consisting of Laminaria hyperborean', Laminaria japonica', Laminaria digitata, Macrocystis pyrifera and Laminaria maxima. Each possibility represents a separate embodiment of the present invention. According to certain exemplary embodiments, the Durvillaeaceae seaweed is ^DurviHaea species. According to further certain exemplary embodiments, the Lessoniaceae seaweed is a Lessonia species. The bacterial strains and homologs thereof are as described hereinabove. According to certain exemplary embodiments, the seaweed extract is an extract of Acophyllus nodosum. According to further exemplary embodiments, the extract of Acophyllus nodosum forms part of commercial product ExpertGrow (ADAMA, Brazil) .According to some embodiments of the present invention, a plurality of bacteria of the strains of the present invention and / or functional homologs thereof, each alone or a combination thereof, are combined with at least one additional active agent. According to certain embodiments, the active agent is a pesticidal agent. According to certain exemplary embodiments, the pesticidal agent is a chemical pesticide. According to further additional aspects, the present invention provides a combination of a plurality of isolated Bacillus velezensis strain 0RN14 and / or a functional homolog thereof and isolated Bacillus subtillis strain ORN160 and / or a functional homolog thereof and at least one additional active agent. According to certain embodiments, the active agent is a pesticidal agent. According to certain exemplary embodiments, the pesticidal agent is a chemical pesticide. A variety of chemical pesticides may be added to the compositions of the present invention. Exemplary chemical pesticides include acylalanines, butyrolactones, oxazolidinones, hydroxy-(2-amino-) pyrimidines, isothiazolones, isoxazoles, carboxylic acids, benzimidazoles, thiophanates, N-phenyl carbamates, toluamides, ethylamino-thiazole-carboxamide, phenylureas, pyridinylmethyl-benzamides, aminocyanoacrylates, benzophenone, benzoylpyridine, pyrazole-5-carboxamides, pyrimidinamines, quinazoline, N-methoxy-(phenyl-ethyl)-pyrazole-carboxamides, furan-carboxamides, oxathiin-carboxamides, phenyl-benzamides, phenyl-oxo-ethyl thiophene amide, pyrazole-4-carboxamides, N-cyclopropyl-N-benzyl-pyrazole-carboxamides, pyridinecarboxamides, pyridinyl-ethyl-benzamides, thiazole-carboxamides, pyrazinecarboxamides, benzyl-carbamates, dihydro-dioxazines, imidazolinones, methoxyacetamide, methoxy-acrylates, methoxy-carbamates, oxazolidine-diones, oximino-acetamides, oximino-acetates, tetrazolinones, cyano-imidazole, sulfamoyl-triazole, picolinamides, dinitrophenyl crotonates, 2,6-dinitro-anilines, tri-phenyl tin compounds, thiophene-carboxamides, triazolo-pyrimidylamine, anilino-pyrimidines, enopyranuronic acid, hexopyranosyls, glucopyranosyls, tetracycline, aryloxy quinoline, quinazolinone, phenylpyrroles, dicarboximides, dithiolanes, phosphoro-thiolates, aromatic hydrocarbons, 1,2,4-thiadiazoles, carbamates, piperidinyl-thiazole-isoxazolines, imidazoles, piperazines, pyridines, pyrimidines, triazoles, triazolinthiones, morpholines, piperidines, spiroketal-amines, amino-pyrazolinone, hydroxyanilides, allylamines, thiocarbamates, peptidyl pyrimidine nucleoside, cinnamic acid amides, mandelic acid amides, valinamide carbamates, isobenzo-furanone, pyrrolo-quinolinone, triazolobenzo- thiazole, carboxamide, cyclopropane-carboxamide, propionamide, trifluoroethylcarbamate, benzothiadiazole, thiadiazole-carboxamide, ethyl phosphonates, cyanoacetamide-oxime, phthalamic acids, benzotriazines, benzene-sulfonamides, pyridazinones, phenyl-acetamide, guanidines, cyano-methylene-thiazolidines, pyrimidinone-hydrazones, 4-quinolyl-acetates, tetrazolyloximes, dithiocarbamates, phthalimides, chloronitriles, sulfamides, bis-guanidines, triazines, quinones, quinoxalines, maleimide and phthalonitriles, as well as copper fungicides (acypetacs-copper, Bordeaux mixture, Burgundy mixture, Cheshunt mixture, copper acetate, basic copper carbonate, copper hydroxide, copper naphthenate, copper oleate, copper oxychloride, copper silicate, copper sulfate, basic copper sulfate, copper zinc chromate, cufraneb, cuprobam, cuprous oxide, mancopper, oxine-copper, saisentong, thiodiazolecopper) and other fungicides, including dimethomorph, fosetyl-Al, tetraconazole, azoxystrobin, propineb, pyraclostrobin, potassium phosphite, mefenoxam, and folpet. According to an aspect of the invention, there is provided a bacterial preparation comprising a plurality of bacteria of at least one bacterial strain selected from the group consisting of Bacillus velezensis strain ORN14, Bacillus subtillis strain ORN160, functional homolog thereof and a combination of same, wherein the bacterial preparation is characterized by a pesticidal activity against at least one plant nematode, and / or plant pathogenic fungus, and / or insect plant pest. According to certain embodiments, the plurality of bacteria of the at least one bacterial strain or functional homolog thereof is present in the preparation at a concentration which is enriched over that (exceeds that) found in nature. In nature, the bacterial strain is typically part of the plant microbiome, consisting of more than thousands of microbial species, whether in the phyllosphere, including endophytes, rhizoplane, and rhizosphere, or in any other plant compartment. The natural habitat of the bacterial strain can also be the insect or mite pests (insect / mite microbiome). According to some embodiments of the invention, the preparation comprises less than 50, 20, 10, 9, 8, 7, 6, 5, or 4 microbial species, e.g., bacteria and fungi, particularly bacteria other than bacterial strains of the invention. According to certain embodiments, the microbial preparations comprise 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 microbial species. Each possibility represents a separate embodiment of the present invention. According to certain exemplary embodiments, the microbial preparation comprises a single microbial species, i.e., a bacterial strain according to the teachings of the present invention. According to certain exemplary embodiments, the preparation comprises a single strain of the present invention, wherein said preparation is devoid of other microbial species. According to certain further exemplary embodiments, the preparation comprises a plurality of strains of the present invention, wherein said preparation is devoid of other microbial species. According to certain embodiments, the preparation comprises the bacterial strain of the invention at a level of purity of at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 85%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95% or more, say 100% pure. As used herein, the term “pure” or “purity” refers to the percentage of the bacterial strain of the invention out of the total number of microorganisms in the preparation. According to certain exemplary embodiments, the preparation comprises the bacterial strain of the invention at a level of purity of at least about 99%, at least about 99.1%, at least about 99.2%, at least about 99.3%, at least about 99.4%, at least about 99.5%, at least about 99.6%, at least about 99.7%, at least about 99.8%, at least about 99.9%, at least about 99.95%, at least about 99.99%, at least about 99.995%, at least about 99.999% or more, say 100% pure. As used herein, the term “enriched” refers to 2-10xl06-fold enrichment over that found in nature in an isolate of microbiota obtained from a plant phyllosphere and / or rhizosphere comprising a strain of the invention or a functional homolog of same. According to certain embodiments, the bacterial preparation comprises viable bacterial cells (capable of replicating). According to some embodiments, the bacterial strain comprises sporulating bacteria. According to some embodiments, the viable bacterial cells are in a dormant state. According to some embodiments, the isolated bacterial strain is in a sporulated form. According to some embodiments, the bacterial preparations of the invention comprise non-viable forms of the bacterial strains of the invention. According to certain embodiments, the non-viable form comprises non-sporulating bacteria. According to some embodiments, the non-viable form comprises dried bacterial strains. A “spore” or “spores” refers to microbial structures that are generally viable, more resistant to environmental influences such as heat and bactericidal agents than other forms of the same microbial species, and typically capable of germination and out-growth. Bacteria that are “capable of forming spores” are those bacteria comprising the genes and other necessary abilities to produce spores under suitable environmental conditions. According to further certain aspects, the present invention provides an agricultural composition comprising a plurality of bacteria of at least one isolated bacterial strain selected from the group consisting of Bacillus velezensis strain 0RN14 and / or a functional homolog thereof; Bacillus subtillis strain ORN160 and / or a functional homolog thereof; and a combination thereof, a preparation of same, a lysate, a broth or an extract thereof. Each possibility represents a separate embodiment of the present invention. According to some aspects of the invention there is provided a formulation comprising the bacterial preparation, or the agricultural composition, optionally comprising the preparation, as described herein. Any carrier suitable for agricultural use can form part of the agricultural compositions and / or formulations of the present invention. The carrier may be any one or more of a number of carriers that confer a variety of properties, including increased stability, wettability, dispersability, etc. Wetting agents such as natural or synthetic surfactants, which can be nonionic or ionic surfactants, or a combination thereof can be included in a composition of the invention. Water-in-oil emulsions can also be used to formulate a composition that includes at least one isolated microorganism of the present invention (see, for example, U.S. Patent No. 7,485,451). Suitable formulations that may be prepared include wettable powders, granules, gels, agar strips or pellets, and the like, microencapsulated particles, and the like, liquids such as aqueous flowables, aqueous suspensions, water-in-oil emulsions, etc., as detailed hereinabove. The formulation may include grain or legume products (e.g., ground grain or beans, broth or flour derived from grain or beans), starch, sugar, or oil. The carrier may be an agricultural carrier. In certain preferred embodiments, the carrier is a seed, and the composition may be applied or coated onto the seed or allowed to saturate the seed. According to some embodiments, the agricultural carrier may be soil or plant growth medium. Other agricultural carriers that may be used include water, plant-based oils, humectants, or combinations thereof. Alternatively, the agricultural carrier may be a solid, such as diatomaceous earth, loam, silica, alginate, clay, bentonite, vermiculite, seed cases, other plant and animal products, or combinations, including granules, pellets, or suspensions. Mixtures of any of the aforementioned ingredients are also contemplated as carriers, such as but not limited to, pesta (flour and kaolin clay), agar or flour-based pellets in loam, sand, clay, etc. Formulations may include food sources for the cultured organisms, such as barley, rice, or other biological materials such as seed, plant parts, sugar cane bagasse, hulls or stalks from grain processing, ground plant material ("yard waste") or wood from building site refuse, sawdust or small fibers from recycling of paper, fabric, or wood. Other suitable formulations will be known to those skilled in the art. In the liquid form, e.g., solutions or suspensions, the microbial strain may be mixed or suspended in water or in aqueous solutions. Suitable liquid diluents or carriers include water, aqueous solutions, petroleum distillates, or other liquid carriers. Solid compositions can be prepared by dispersing the microbial strain in and on an appropriately divided solid carrier, such as peat, wheat, bran, vermiculite, clay, talc, bentonite, diatomaceous earth, fuller's earth, pasteurized soil, and the like. When such formulations are used as wettable powders, biologically compatible dispersing agents such as non-ionic, anionic, amphoteric, or cationic dispersing and emulsifying agents can be used. According to some embodiments the at least one bacterial strain of the invention may be applied in a delayed-release composition. According to some embodiments the at least one bacterial strain of the invention may be applied in a sustained release composition. According to some embodiments the bacterial strain may be applied in a dehydrated form, and the release thereof may be initiated upon contact with water, or at a predetermined lag time therefrom. Each possibility is a separate embodiment. Any fertilizer as is known in the art can be added to the compositions / formulations of the present invention, as long as the fertilizer does not interfere with the bacterial growth and activity. According to certain embodiments, the fertilizer is selected from the group consisting of chemical or biological fertilizer. The amount of the at least one chemical or biological fertilizer employed can vary depending on the final formulation as well as the size of the plant and / or seed to be treated. The formulation as used herein can also refer to a customary formulation in an effective amount to be applied either to the soil (e.g., in-furrow), to a portion of the plant (e.g., drench) or on the seed before planting (e.g., seed coating or dressing). Customary formulations include, for example, solutions, emulsifiable concentrates, wettable powders, suspension concentrates, soluble powders, granules, suspension-emulsion concentrates, natural and synthetic materials impregnated with active compounds, and very fine controlled release capsules in polymeric substances, as detailed above. In certain embodiments of the present invention, the microbial strains are formulated in powders that are available in either a ready-to-use formulation or are otherwise mixed together at the time of use. In either embodiment, the powder may be admixed with the soil prior to or at the time of planting. Depending on the final formulation, one or more suitable additives can also be introduced to the compositions of the present invention. Adhesives such as carboxymethylcellulose and natural and synthetic polymers in the form of powders, granules or latexes, such as gum arabic, chitin, polyvinyl alcohol and polyvinyl acetate, as well as natural phospholipids, such as cephalins and lecithins, and synthetic phospholipids, can be added to the compositions / formulation of the present invention. According to certain embodiments, the bacterial strains are formulated in a single, stable solution, or emulsion, or suspension. For solutions, the chemical compounds are typically dissolved in solvents before the microbial strain is added. Suitable liquid solvents include petroleum-based aromatics, such as xylene, toluene or alkylnaphthalenes, aliphatic hydrocarbons, such as cyclohexane or paraffins, for example petroleum fractions, mineral and vegetable oils, alcohols, such as butanol or glycol as well as their ethers and esters, ketones, such as methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, strongly polar solvents, such as dimethylformamide and dimethyl sulphoxide. For emulsion or suspension, the liquid medium is water. In some embodiments, the chemical agent and the microbial strain are suspended in separate liquids and mixed at the time of application. In some particular embodiments of suspension, the chemical agent and the microbial strain are combined in a ready-to-use formulation that exhibits a reasonably long shelf-life. In use, the liquid can be sprayed or can be applied to the plant foliage as an atomized spray or in-furrow at the time of planting the crop. The liquid composition can be introduced in an effective amount on the seed (i.e., seed coating or dressing) or to the soil (in-furrow) before germination of the seed or directly to the soil in contact with the roots by utilizing a variety of techniques known in the art including, but not limited to, drip irrigation, sprinklers, soil injection or soil drenching. Each possibility represents a separate embodiment of the invention. Optionally, stabilizers and buffers can be added, including alkaline and alkaline earth metal salts and organic acids, such as citric acid and ascorbic acid, inorganic acids, such as hydrochloric acid or sulfuric acid. According to some preferable embodiments, the amount of the bacterial strain or functional homolog within the agricultural composition / formulation is sufficient to interact, colonize and / or localize in a cultivated plant treated with same. One of ordinary skill in the art would know how to calculate the concentration of the microbial strain or functional homolog. According to certain embodiments, the bacterial strain(s) is about 2% w / w to about 95% w / w of the entire formulation / agricultural composition. According to further embodiments, the bacterial strains(s) employed in the compositions is about 5% w / w to about 90% w / w, about 5% to about 15%, about 5% to about 10%, about 10% to about 60%, about 10% to about 40%, about 10% to about 25%, about 15% to about 35%, about 20% to about 50%, about 20% to about 35%, about 25% to about 45%, about 60% to about 90%, about 60% to about 75%, about 70% to about 90%, or about 80% to about 90%, by weight of the entire formulation / composition, including each value within the specified ranges. Each possibility represents a separate embodiment of the invention. According to certain embodiments, the preparation / composition provided herein is formulated to provide stability for the bacterial strain or functional homolog. Optionally, a shelf-stable formulation is in a dry form, e.g., a powder formulation, or a lyophilized formulation. According to certain embodiments, the formulation is substantially stable at temperatures between about -20°C and about 50°C for at least about 1, 2, 3, 4, 5, or 6 days, or 1, 2, 3 or 4 weeks, or 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12 months, or one or more years. In other embodiments, the formulation is substantially stable at temperatures between about 4°C and about 37°C for at least about 5, 10, 15, 20, 25, 30 or for about 180 days and more. According to certain exemplary embodiments, the formulation is substantially stable at temperatures between about 4°C and about 37°C for at least about 30 days. According to further exemplary embodiments, the formulation is substantially stable at a temperature range of from about 20°C to 25°C for more than 30 days. According to certain additional exemplary embodiments, the formulation is substantially stable at a temperature range of from about 20°C to 25°C for at least 6 months, at least 12 months, at least 18 months or about 24 months when in a form of SC, WDG or WP. According to certain exemplary embodiments, the microbial strain or functional homolog may be shelf-stable, wherein at least 0.01% of the CFU or spores are viable after storage in desiccated form (i.e., moisture content of 30% or less) for 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more than 10 weeks at 4°C or at room temperature. According to yet additional certain aspects, the present invention provides a seed coated with the pesticidal composition comprising at least one bacterial strain, a lysate, an extract, or a broth thereof, or a combination thereof with at least one active pesticidal agent, as described herein. According to certain aspects, the present invention provides a container adapted for a watering system of a plant field, comprising the pesticidal composition comprising at least one bacterial strain, a lysate, an extract, or a broth thereof, or a combination thereof with at least one active pesticidal agent, as described herein. According to certain aspects, the present invention provides a kit comprising (i) a pesticidal composition comprising at least one bacterial strain, a lysate, an extract, or a broth thereof, or a combination thereof with at least one active pesticidal agent, as described herein; optionally (ii) a delivery system for applying the pesticidal composition to a plant or a part thereof or to the plant growth habitat and (iii) instructions for using the agricultural composition. As used herein, the phrase “CFUs” or “Colony Forming Units” refers to the number of microbial cells in a defined sample (e.g., milliliter of liquid, square centimeter of surface, one seed of grain, etc.) that form colonies and are thereafter numbered, on a semisolid bacteriological growth medium. According to certain embodiments, the concentration in the composition e.g., preparation, formulation, coated seed etc. is 102 CFU to 109 CFU / seed or 102 CFU-109 CFU / gr powder or 102 CFU-109CFU / ml. According to some embodiments, the bacterial strain is applied to seeds at a concentration range of from about 102 CFU / seed to about 107 CFU / seed. According to some certain embodiments, the preparation is in a form selected from the group consisting of a liquid culture, a still (plate or non-shaking liquid) culture, whole culture stored stock of cells (particularly glycerol stocks), agar strip, stored agar plug in glycerol / water, freeze dried stock, and dried stocks such as lyophilizate dried onto filter paper or grain seed. As used herein, the term “culture” refers to a fluid, pellet, scraping, dried sample, lyophilizate or a support, container, or medium such as a plate, paper, filter, matrix, straw, pipette or pipette tip, fiber, needle, gel, swab, tube, vial, particle, etc. that contains the deposited strain or the functional homolog thereof in an amount that exceeds that found in nature, as described hereinabove. In the present invention, an “isolated culture” of a microbial strain is a culture fluid or a scraping, pellet, dried preparation, lyophilizate, or a support, container, or medium that contains the deposited strain or the functional homolog thereof, in the absence of other microorganisms. According to further certain aspects, the present invention provides a method for inhibiting or preventing the growth and / or the development of at least one nematode and / or plant pathogenic fungi and / or insect pest present in or on a plant or a part thereof or a plant habitat, comprising contacting the plant or part thereof or plant habitat with at least one bacterial strain selected from the group consisting of Bacillus velezensis strain ORN14 and / or a functional homolog thereof; Bacillus subtillis strain ORN160 and / or a functional homolog thereof; and a combination thereof, a preparation of same, a lysate, a broth or an extract obtained therefrom or a composition comprising same. Each possibility represents a separate embodiment of the present invention. According to yet further certain aspects, the present invention provides a method for protecting a plant or a part thereof from a disease or deleterious symptoms associates with the growth and / or the development of at least one nematode and / or plant pathogenic fungi and / or insect pest in or on the plant or a part thereof or the plant habitat, comprising contacting said plant or part thereof or plant habitat with at least one bacterial strain selected from the group consisting of Bacillus velezensis strain 0RN14 and / or a functional homolog thereof; Bacillus subtillis strain ORN160 and / or a functional homolog thereof; and a combination thereof, a preparation of same, a lysate, a broth or an extract obtained therefrom or a composition comprising same. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the plant part is selected from the group consisting of a seed, a root, a shoot, a leaf, a branch, a flower, a fruit and any combination thereof. Each possibility represents a separate embodiment of the present invention. Any method as is known in the art can be used to contact the plant or part thereof with the at least one bacterial strain or functional homolog thereof of the present invention. According to certain embodiments, the plant or part thereof is contacted with a preparation / agricultural composition / formulation comprising the at least one bacterial strain or functional homolog thereof by a method selected from the group consisting of, but not limited to, infiltration, immersion, dipping, incubation, spraying, coating, dusting, and any combination thereof. According to other embodiments, the plant or part thereof is contacted with the at least one bacterial strain or functional homolog thereof through the plant rhizosphere. According to these embodiments, the preparation / agricultural composition is applied to the plant rhizosphere. According to certain exemplary embodiments, the plant part is a seed and contacting is affected by seed coating. According to some embodiments, the seeds are pre-sized before coating, and / or are dried and transferred to a sizing machine for sizing after coating. According to certain exemplary embodiments, the plant part is a leaf and contacting is performed by spraying or dusting. According to certain exemplary embodiments, the plant part is a root and contacting is performed by dipping or immersing. According to certain exemplary embodiments, the plant part is a seed or a root, and contacting is performed by applying the bacterial strain or functional homolog thereof, preparations, extract, lysate, whole cell broth or a composition comprising same, to the seed or root growth medium. According to some embodiments, the composition may be applied to the plant habitat. According to certain embodiments, the plant habitat is a growth medium, which can be a solid or a liquid growth medium. According to certain exemplary embodiments, the solid growth medium is soil. According to these embodiments, the pesticidal composition of the invention is applied to the soil by a method selected from the group consisting of in furrow application, broadcast spray pre-planting incorporation, broadcast spraying on the soil surface, drip irrigation, soil injection, soil drenching, and any combination thereof. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the plant or part thereof is surface sterilized prior to contacting with the bacterial preparation / composition / formulation, particularly for research applications. Any means for applying the bacterial strains and / or functional homologs thereof, preparations, lysates, extracts, whole cell broth and / or compositions comprising same, as such means are known in the art, can be used with the teachings of the present invention. According to some embodiments, wherein the application is to the aerial part of a plant, application may be mediated by beneficial insects, for example bees, exposed to the bacterial strains and / or their formulations. According to some embodiments, the bacterial strains and / or formulations comprising same are at least partially coating the beneficial insects. Application to the plant aerial part may also be mediated by moving vehicles (cars or agricultural vehicles), flying devices (e.g., drones), and the like. According to some embodiments, a single dose of the bacterial strains and / or formulations comprising same of the present invention is applied to the plant or part thereof for inhibiting or preventing the growth and / or the development of a plant pest. According to some further embodiments, a plurality of doses of the bacterial strains and / or formulations comprising same are applied to the plant or part thereof, according to need, e.g., higher or more frequent delivery of doses in correlation to the severity of the pest infection or threat thereof. According to some further embodiments, a plurality of doses of the bacterial strains and / or formulations comprising same are applied to the plant or part thereof at particular set intervals. For example, doses of the bacterial strains and / or formulations comprising same may be applied every 1 to 30 days, e.g., every 1, 2, 3, 4, 5, 7, 10, 15, 20 or 30 days, or any other value within the specified range; may be applied every 1 to 6 weeks, e.g., every 1, 2, 3, 4, 5 or 6 weeks; and / or may be applied every 1 to 12 months, e.g., every 1, 2, 3, 4, 6, 9, or 12 months, or any other value within the specified range. Each possibility represents a separate embodiment of the invention. According to some particular embodiments, a dose of the bacterial strains and / or formulations comprising same is applied to the plant or part thereof every week, every 10 days, every 2 weeks, or every month. Each possibility represents a separate embodiment of the invention. Application of a dose at particular set intervals is usually confined to a limited time period, e.g., for 30 days, for 3 months, and the like. For example, according to some embodiments, a dose of the bacterial strains and / or formulations comprising same is applied every 10 days for a period of 30 days. According to another exemplary embodiment, a dose of the bacterial strains and / or formulations comprising same is applied every two weeks for a period of 3 months. According to some further embodiments, when a combination comprising the bacterial strains and / or formulations comprising same and at least one additional active agent, as described above, is applied to a plant or part thereof, doses of the respective active components of the combination are applied concurrently, i.e., in the same composition, or at the same time, or on the same day. According to some further embodiments, doses of the active components of a combination according to the present invention may be applied separately, e.g., at different times of day or on different days. According to some further embodiments, a dose of a first active component of the combination may be applied to the plant or part thereof following a particular lag-time from the application of a dose of a second active component. Some non-limiting examples a of lag-time include about 6 hours, about 12 hours, about 1 day, about 2 days, about 3 days, or more. Each possibility represents a separate embodiment of the invention. According to some further embodiments, the number of doses of each active component which is applied to the plant or part thereof may differ. For example, according to some embodiments, 3 doses of the first active component of the combination are applied, e.g., at intervals of 10 days between doses, and 2 doses of the second active component are applied, e.g., every 15 days. According to another exemplary embodiment, 2 doses of the second active component are applied every 10 days, between the applications of the 3 doses of the first component. According to another exemplary embodiment, 2 doses of the second active component are applied every 10 days together with the first and second, or second and third, applications of the 3 doses of the first component. These embodiments are non-limiting examples, and any other number of doses, and / or respective intervals between the applications of the doses of bacterial strains and / or formulations comprising same, combinations comprising same, or components thereof, are contemplated and disclosed herein. In some embodiments, the bacterial preparation, compositions and / or formulations comprising same can be contacted with the plant or part thereof, or the plant habitat, for example the plant seed and / or plant aerial parts, and successful colonization can be confirmed by detecting the presence of the microbial strain within the plant. For example, after applying the composition / formulation / preparation to the seeds, high titers of the microbial strain can be detected in the roots and shoots of the plants that germinate from the seeds. In addition, significant quantities of the microbial strain may be detected in the rhizosphere of the plants. Therefore, in some embodiments, the microbial strain is applied in an amount effective to colonize the plant. The microbial strain may be colonized on the surface of the plant or within the plant tissues as an endophyte. In some embodiments, colonization of the plant can be detected, for example, by detecting the presence of the microbial strain inside the plant. This can be accomplished by measuring the viability of the microbial strain after surface sterilization of the plant portion: microbial strain colonization results in an internal localization of the microbe, rendering it resistant to conditions of surface sterilization. According to some embodiments, the microbial strain is applied in an amount effective to colonize the plant rhizosphere. The presence and quantity of the microbial strain can also be established using other means known in the art, for example, immunofluorescence microscopy using microbe-specific antibodies, or fluorescence of in situ hybridization. Alternatively, specific nucleic acid probes recognizing conserved sequences from the colonized bacterial strain can be employed to amplify a region, for example by quantitative PCR, and correlated to CFUs by means of a standard curve. According to certain exemplary embodiments, the bacterial strain can be detectable within a target tissue of the mature cultivated plant selected from a fruit, a seed, a leaf, or a root, a portion thereof and a combination thereof. It is to be explicitly understood that the bacterial strain or the functional homolog can colonize a plant part distinct from the plant part with which said bacterial strain or functional homolog was contacted. According to certain embodiments, the bacterial strain or functional homolog thereof interacting with the plant is present in the plant habitat, particularly in the rhizosphere (soil around root). According to certain embodiments, the bacterial strain or functional homolog thereof interacting with the plant is present on or inside a plant tissue, including, but not limited to, the rhizoplane (root surface), root endosphere (inside the root), stem endosphere (inside the stem), leaf endosphere (inside the leaf), phyllosphere (on the shoot, stem and leaf surface), seed surface and seed endosphere (inside the seed). Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the bacterial strain contacted with the plant, part thereof or the plant rhizosphere is not detected in said plant, plant part thereof and rhizosphere prior to application of said bacterial strain. According to other embodiments, the bacterial strain is naturally present in the plant, part thereof, and / or plant rhizosphere. In any case, the amount of the bacterial strain in the plant, part thereof or plant rhizosphere is higher after contacting with the strain compared to its amount prior to contacting (application) .As used herein and in the claims section below, the phrases “inhibiting the activity and / or growth and / or development, and / or in killing” and “having pesticidal activity” are used herein interchangeably and refer to an effective amount of the bacterial strain(s) and / or homolog(s) thereof with or without additional agents according to the teachings of the invention which is capable of killing or inhibiting the development of at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or 100% of a population of the nematode and / or fungus and / or insect as compared to the population of a nematode and / or fungus and / or insect of the same species not exposed to / being in contact with / consuming the effective amount of the bacterial strain(s) and / or homolog(s) thereof or combination of same with additional agent(s) when growing under the same (e.g., identical) conditions; and / or when compared to the initial population of the nematode and / or fungus and / or insect prior to being exposed to / contacted with / fed with the agents of some embodiments of the invention. Methods of qualifying pesticidal activity are known in the art and are further described and exemplified hereinbelow. In addition, IC50 values can be determined to qualify effective concentration of the agent resulting in inhibiting growth and development of at least 50% of the pest population. According to certain embodiments, the phrases “inhibitory activity” and / or “inhibiting the development of a pest”, which are interchangeably used herein, refer to an activity which results in reducing the size and / or mass (e.g., stunting) of the pest (e.g., nematode) as compared to the size and / or mass of a pest of the same species in the absence of the effective amount of the agent under the same (e.g., identical) growth conditions; and / or when compared to the size and / or mass of the pest prior to being contacted with the bacterial strains of the invention. The inhibitory activity can be direct, i.e., resulting from consumption by the pest, or indirect, i.e., resulting from application to the plant of the bacterial strain(s), extract, lysate, whole cell broth and the like according to the teachings of the invention. According to certain embodiments, a strain of the invention is examined for its capability to inhibit the activity and / or development of, or to kill, a pest, by assessing the symptoms associated with the presence of the pest in or on a plant, a part thereof or its habitat in a control plant compared to a plant treated with the bacterial strain. The control plant is typically, but not necessarily, of the same species as the treated plant. According to some embodiments, the control plant is of the same species and has the same genetic background as the treated plant. The capability can be manifested as an increase of 0.1%, 0.2%, 0.3%, 0.5%, 0.75%, 1%, 1.5%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 15%, 17%, 20%, 25%, 30%, 35%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, or more in 55 health, growth, multiplication, fertility, vigor, strength, or yield, as compared to a control plant. The capability can be a decrease of 0.1%, 0.2%, 0.3%, 0.5%, 0.75%, 1%, 1.5%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 15%, 17%, 20%, 25%, 30%, 35%, 40%, 50%, 60%, 70%, 80%, 90%, or 100% in the symptoms associated with the presence of the insect or mite. According to certain exemplary embodiments, the treated plant and the control plant are grown under the same conditions. Symptoms associated with the presence of a pest in or on a plant or its habitat are known to a person skilled in the art. Typically, a score scale is set for a certain symptom associated with the presence of the pest based on symptom description and / or severity. Optionally, a specific score scale is set for a combination of certain symptoms and a plant species. The use of score scale simplifies the comparison of symptoms in plants subjected to various treatments. According to certain embodiments, each of the isolated strains may be additionally or alternatively characterized by at least one strain-specific genomic marker. According to certain exemplary embodiments, identity of a genomic marker sequence is defined as at least 90% query coverage with at least 95% identity. According to certain specific embodiments, a functional homolog of the bacterial strains of the invention may be further or alternatively identified by comprising at least one genomic marker having at least about 96%, at least about 97%, at least about 98%, at least about 99%, at least about 99.5%, at least about 99.9% or more homology, or identity, to a genomic marker of a strain of the present invention, over at least 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, or at least about 97% query coverage. Each possibility represents a separate embodiment of the present invention. According to certain additional or alternative embodiments, the genomic nucleic acid sequences of the bacterial strain and the functional homolog thereof comprises at least one shared marker. According to certain embodiments, the microbial strain of the present invention or the functional homolog thereof comprises at least two genomic markers, at least three genomic markers, at least four genomic markers, or at least five genomic markers. Each possibility represents a separate embodiment of the present invention. According to additional or alternative embodiments, the strain of the invention and the functional homolog thereof are characterized by substantially the same (+ / - about 10%, 20%, 30%, 40%, 50%, 60% when tested under the same conditions) biochemical profiling (e.g., biochemical fingerprinting) using for example, the GEN III redox chemistry (BIOLOG Inc. 21124 Cabot Blvd. Hayward CA, USA), which can analyze both Gram-negative and Gram-positive bacteria, fortheir ability to metabolize all major classes of biochemicals, in addition to determining other important physiological properties such as pH, salt, and lactic acid tolerance. Further details of biochemical profiling can be obtained in “Modem Phenotypic Microbial Identification”, Bochner B.R., Encyclopedia of Rapid Microbiological Methods, 2006, v.2, Ch. 3, pp. 55-73. Genomic data can be obtained by methods which are well known in the art, e.g., DNA sequencing, bioinformatics, electrophoresis, enzyme-based mismatch detection assay and a hybridization assay; including, e.g., PCR, RT-PCR, Rnase protection, in-situ hybridization, primer extension, Southern blot, Northern Blot and dot blot analysis and the like. The following examples are presented in order to more fully illustrate some embodiments of the invention. They should, in no way be constmed, however, as limiting the broad scope of the invention. One skilled in the art can readily devise many variations and modifications of the principles disclosed herein without departing from the scope of the invention. EXAMPLES Example 1: ORN14 and ORN160 morphology Figure 1 and Table 1 below show the growth and appearance of the novel strains of the invention. Table 1: ORN14 and ORN160 morphology Strain Colony Appearance Colony Size Colony Color Colony Edge ORN14 Dry, with well-defined center Medium Cream Regular ORN160 Dry, without defined center, with Medium Cream Regular very marked border between colonies when same meet Example 2: Assembly of the genome and phylogenomic study of ORN 14 and ORN 160 • Reading quality and cutting parameters: FastQC • Filtering: trimmomatic • Reassembly: Hybrid IDBA software and SPADES • Mounting Comparison: SPADES • Alignment and generation of scaffolds: webserver CONTIGuator - ORN 14: Bacillus velezensis NKG-1 reference genome. - ORN 160: Bacillus subtilis BS 16045 reference genome. • Gap filling: Manual with reading mapping using Bowtie 2 software and filling of gaps using CLC Genomics Workbench 12 GUI. • Genome start: Comparison with the reference strain and considering DnaA as the first gene. • Circular genome: BRIG software (BLAST Ring Image Generator). • Cluster prediction: antiSMASH 6.0.1 webserver. • Similarity: OAT (OrthoANI) = the ANI (Average Nucleotide Identity) and dDDH (digital DNA-DNA hybridization). Heatmap and supplementary graphics: The orthoANI matrix data and information generated by the orthoANI software itself were exported and used to make trees and heatmap. The graphics were generated using the ggplot2 package of the R software. 2,1 Genomic and phylogenomic characterization of ORN 14 Figure 2 shows its Phylogenomic Tree. Table 2 presents conserved regions within ORN 14 genome. Table 2: Conserved regions within 0RN14 genome Region Type From To Most similar known cluster Similarity Region 1 ihiopepiide G?. LAP £$ 230.513 310.240 WS£? 331071 388,153 surfactin fit NRTtipopeptiSs 82¾ ifeysn 3 s RKS-iiketf §18.820 969,064 Oulirosin A? fati&osin 3 Gt Sacchantie 1% Region® I sergeneS' 1.045.698 1,062.861 Region 5 tran$A~PKS Gt UMSOO 1,481,235 oiacroiactin H fit PQlykgsidQ !!!M 1 Regions transA>FKS Et,. T3PKS Gt. NRPS Gt 1,(187,438 1,?8?,8?9 bacifeena Ef PGlyWide + MRP lllll Region? NRPS fit. InansAT-FKS Gf. betaiactone & 1.856.282 1,933.65? fengydn Of NRP lli® Regions? I ietgeoe & 2,016.604 2,033,5?? Region S T3PKS t? 2JCO.S52 2,145,552 Region IQ transAT-PKS & 2.W52 2.370,744 d«n fit Polyket^ NPP Illis Region 1i NRPS Gt. RiPFMike G? 2.030.665 3,041,456 biscllibaain & 100% Region 52 1 other fit 3,567,838 3,869,356 badfysin at ■ffli Based on the above analyses, ORN 14 has been identified as Bacillus velezensis. 22 Genomic and phvlogenomic characterization of ORN160 5 Figure 3 shows its Phylogenomic Tree. Table 3 presents conserved regions within ORN 160 genome. Table 3: Conserved regions within ORN160 genome terpen G? sa-sAtas S' {Wte a?: TWS fit. W$ fit s'.. er Regi^?: TsRKSt? srp§ sr fit MS' tTs.S’i i.500,265 GW28 N’x / N tStaj 3S®T 2.953249 3,392.1 33 ?.2?i.90g OSTS 5.333.491 S SOS? Jis'-.O 3393.844 yss..o SOS'® O® 8359® ^st simiiar know Tester Oisniispty iiii aaassensgee?:-: Res5? ? nesUsTSs A Ct IsRS iiiii SsTaere St Illi® fengycsi i? tsR:’’ 1111® jeceWt'S S' iii®i SyTissinA 1? RTF SSsSSTT iiiM SacTssi S' OS's iii® Based on the above analyses, ORN 160 has been identified as Bacillus subtillis. Example 3: Efficiency of ORN 14 and ORN 160 in the control of Meloidogyne javanica in soybean To test the pesticidal efficacy of the bacterial strains according to the present invention, nematode-infected seeds were treated with the bacterial strains according to the invention, and nematode load in the emerged plant roots was examined. Soybean seeds (cv. M6410IPRO) were pre-treated with Shelter® (fipronil 250 g / 1, FS formulation type, 200 mL / 100 kg seeds) and Kilate® (carbendazim 150+captan 350 g / 1, FS formulation type, 250 mL / 100 kg seeds), so as to prevent plant-disease or other plantafflictions which were not related to the experiment. The seeds were inoculated with the nitrogen-fixing bacteria Bradyrhizobium japonicum, and were then divided into 4 groups (including a negative control and a positive control), each group treated with a respective formulation including at least one active ingredient (A.I.) as shown in Table 4 below (herein "biologically treated seeds"): Table 4: Biological treatment of the seed groups No. Product Formulation Active Ingredient (AI.) ml or g / lOOKg seed Application 1. Negative Control - - - - 2. Positive Control 200 + 200 g / 1 (WG formulation type) B. sub tills + B. licheniformis 150 Seed Treatment 3. ORN 14 333 g / 1 (SC formulation type) B. velezensis 200 Seed Treatment 4. ORN 160 333 g / 1 (SC formulation type) B. sub tills 200 Seed Treatment A plurality of polystyrene pots containing 0.5 L of an autoclaved mixture of soil and sand (at a ratio of 2:1) were provided, and a suspension, containing a biologically treated soybean seed (from one of the groups above) and approximately 2,000 eggs and second-stage juveniles (J2) of Meloidogyne javanica, was placed within a hole in the soil of each respective pot, about 2.5 cm deep. Fifty-five days after inoculation, the plants were removed from the pots, and the shoots were separated from the roots. For evaluation of nematode presence, the roots were washed and weighed. The roots were then submitted to a nematode extraction process according to the method of Boneti and Ferraz (Boneti, J.I.S. and Ferraz, S, (1981) Brazilian Plant Pathologhy 6, 553). The samples obtained from the extraction process were examined in a perfusion chamber, under a light microscope, to evaluate the amount of Meloidogyne javanica (Mj) present in the roots. This was divided by root mass, obtaining the nematode parameter per gram of root. The Reproduction Factor (RF), representing the final population divided by the initial population of the nematodes, was also calculated. The data obtained was submitted to analysis of variance and the averages were compared by Scott Knott algorithm (P value<0.05). The results are presented in Table 5 below. Compact letter display (CLD) illustrates statistically significant differences between the mean values, and the percentage of total nematode reduction relative to the control group is denoted "%RRT". Table 5: Nematode growth in seed groups Treatments Mj total %RRT by Mj total Mj / g of root %RRT by Mj / g of root RF Negative Control 101,953 a - 27,624 a - 50.97 a Positive Control 42,813 b 58.01 5,495 c 80.11 21.38b ORN 14 35,901 b 64.79 4,723 c 82.90 17.96 b ORN 160 47,558 b 53.35 6,400 c 76.83 23.80 b As is shown in the table above, treatment with each of ORN 14 and ORN 160 strains surprisingly produced a reduction of more than 50% in the total number of M. javanica compared to the untreated control group, ORN 14 obtaining a 64.79% reduction. The effect was even more substantial when measured by the number of nematodes per gram of root, achieving a reduction of more than 75%. Example 4: Efficiency of combinations of ORN 14 with additional active agent in the control of Sclerotinia sclerotiorum in soybean To test the pesticidal efficacy of the bacterial strains according to the present invention in combination with an additional active agent, plants were treated with the bacterial strains of the invention, alone and in combination with an exemplary active agent, and emergence of mold was subsequently examined. Soybean plants (cv. M6410 IPRO) were divided into 8 groups, including negative and positive control groups, a groups treated with bacterial strains according to the present invention, a group treated with a known fungicidal active agent, and a group treated with a combination of strains according to the present invention and the additional active agent. Each plant group contained at least 80 plants and spanned an area of at least 15 m2, the areas located in randomized blocks. The treatment formulations included the respective at least one active ingredient (A.I), mixed within an adjuvant derived from soybean oil and containing 765 g / 1 of soybean oil methyl ester. The formulations were applied to the plants at intervals of 10 days, i.e., at soybean growth stage RI (denoted stage “B”), 10 days after RI (denoted stage “C”), and in some cases also at soybean growth stage V3 (denoted stage “A”), 10 days prior to RI. All regionally recommended plant treatments were performed regularly during the experiment (e.g., for prevention of diseases such as rust and CFD), apart from treatments or products that have a recommendation to control infection by Sclerotinia sclerotiorum (herein referred to as “white mold”). The active ingredients and dosages of the respective formulations are shown in Table 6 below: Table 6: Combination treatment of soybean plants Group No. Product Formulation Active ingredient (AI.) A.I. dose (g) / ha App. stage 1 Negative Control - - - - - 2 Additional A.I. 500 g / 1 SC Fluazinam 375 BC 3 ORN 14 333.33 g / 1 SC Bacillus velezensis 166 ABC 4 ORN 14 333.33 g / 1 SC Bacillus velezensis 333 ABC 5 ORN 14 333.33 g / 1 SC Bacillus velezensis 666 ABC 6 Positive biological control 13.68 g / 1 SC Bacillus subtilis 41.04 ABC 7 ORN 14 Additional A.I. 333+375 g / 1 SC Bacillus velezensis + Fluazinam 333 + 375 BC 8 ORN 14 Additional A.I. 333.33 g / 1 SC 333+375 g / 1 SC Bacillus velezensis, Bacillus velezensis + Fluazinam 333, 333 + 375 A BC Following application of the respective treatments set out above, the fungicidal effectivity was evaluated. Unexpectedly, the bacterial strain ORN14 significantly reduced the incidence of white mold in the plant population at all application dosages, displaying an efficiency of over 25% at the high dosage thereof, and surpassing the efficiency of the positive biological control at both medium and high application dosages (groups 4 and 5 in Table 6, respectively). Moreover, when applied in combination with the additional (chemical) active ingredient, 0RN14 achieved an increase of about 10% in the efficiency of white mold reduction relative to the negative control, reaching an efficiency of almost 80%. Example 5: Efficiency of combinations of ORN 14 and ORN 160 with seaweed extract in the control of pests in soybean To test the pesticidal efficacy of the bacterial strains according to the present invention in combination with seaweed extract, plants are treated as described in Example 3 or in Example 4 above, with the bacterial strains of the invention combined with seaweed extract, and the nematode load and emergence of mold are subsequently examined. The foregoing description of the specific embodiments will so fully reveal the general nature of the invention that others can, by applying current knowledge, readily modify and / or adapt for various applications such specific embodiments without undue 63 experimentation and without departing from the generic concept, and, therefore, such adaptations and modifications should and are intended to be comprehended within the meaning and range of equivalents of the disclosed embodiments. It is to be understood that the phraseology or terminology employed herein is for the purpose of description and 5 not of limitation. The means, materials, and steps for carrying out various disclosed functions may take a variety of alternative forms without departing from the invention.
Claims
1. An isolated bacterial strain or a functional homolog thereof, wherein the isolated bacterial strain being selected from the group consisting of:a) a Bacillus velezensis strain 0RN14, the strain being selected from the group consisting of:(i) a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO: 1;(ii) a strain deposited under ATCC Accession Number PTA-127479; anda combination thereof; andb) a Bacillus subtillis strain ORN160, the strain being selected from(i) a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:2;(ii) a strain deposited under ATCC Accession Number PTA-127478;and a combination thereof.
2. The isolated strain of claim 1, wherein the functional homolog of strain 0RN14 comprises a 16S-rRNA sequence at least 97% identical to SEQ ID NO: 1.
3. The isolated strain of any one of claims 1-2, wherein the functional homolog of strain ORN160 comprises a 16S-rRNA sequence at least 97% identical to SEQ ID NO:2.
4. The isolated strain of any one of claims 1-3, wherein each of the strains 0RN14 and ORN160 is further characterized by at least one strain-specific genomic marker sequence.
5. The isolated strain of any one of claims 1-4, wherein said strain is characterized by a capability to inhibit the growth and / or the development and / or the activity of, or to kill, at least one plant nematode and / or plant pathogenic fungus and / or insect pest, thereby protecting the plant from, or reducing the impact of the harmful effects of, the at least one nematode and / or fungus and / or insect pest.
6. A microbial combination comprising (i) Bacillus velezensis strain ORN14 and / or functional homolog thereof and (ii) Bacillus subtillis strain ORN160 and / or functional homolog thereof according to any one of claims 1-4, wherein the combination has an enhanced capability to inhibit the growth and / or the development and / or the activity of, or to kill, at least one plant nematode and / or plant pathogenic fungus and / or insect pest compared to the capability of each strain alone.
7. The microbial combination of claim 6, wherein the enhanced capability is a synergistic capability.
8. The microbial combination of any one of claims 6-7, wherein said combination comprises the Bacillus velezensis strain ORN14 and the Bacillus subtillis strain ORN160 at a ratio of from about 10:1 to about 1:10.
9. The microbial combination of any one of claims 6-8, wherein said combination comprises the Bacillus velezensis strain ORN 14 and the Bacillus subtillis strain ORN160 ataratio of 1:1.
10. A bacterial preparation comprising a plurality of bacteria of at least one isolated bacterial strain or functional homolog thereof of any one of claims 1-5.
11. The bacterial preparation of claim 10, wherein said preparation comprises a combination of plurality of Bacillus velezensis strain ORN 14 and / or a functional homolog thereof and a plurality of Bacillus subtillis strain ORN160 or a functional homolog thereof, and wherein said preparation has an enhanced pesticidal activity against at least one plant nematode and / or plant pathogenic fungus and / or insect pest compared to a preparation comprising a plurality of each strain alone.
12. The bacterial preparation of any one of claims 10-11, wherein the plurality of bacteria of the at least one bacterial strain or functional homolog thereof is present in said preparation at a concentration which exceeds that found in nature.
13. The bacterial preparation of any one of claims 10-12, wherein the pluralityof bacteria is in a form selected from the group consisting of viable cells, non-viable cells and a combination thereof.
14. A combination of a bacterial preparation comprising a plurality of bacteriaof at least one isolated bacterial strain or functional homolog thereof of any one of claims 1-5 and at least one additional pesticidal active agent.
15. The combination of claim 14, wherein the pesticidal active agent is a chemical pesticide.
16. The combination of any one of claims 14-15, wherein the combination has an enhanced pesticidal activity against at least one plant nematode and / or plant pathogenic fungus and / or insect pest compared to the pesticidal activity of each of the plurality of bacteria and at the least one pesticidal active agent applied alone.
17. A lysate comprising at least one cell or a plurality of cells of the isolated bacterial strain or homolog thereof of any one of claims 1-5 or of the microbial combination of any one of claims 6-9.
18. The lysate of claim 17, said lysate being selected from the group consistingof whole cell lysate and lysate comprising soluble fraction of the at least one cell or the plurality of cells.
19. An extract of at least one bacterial cell of the at least one isolated bacterial strain or functional homolog thereof of any one of claims 1-5 or of a plurality of cells of the microbial combination of any one of claims 6-9.
20. A whole cell broth collected from fermentation of at least one bacterial cell of the at least one isolated bacterial strain or functional homolog thereof of any one of claims 1-5 or of a plurality of cells of the microbial combination of any one of claims 6-9.
21. An agricultural composition comprising at least one isolated bacterial strain or functional homolog thereof of any one of claims 1-5, a microbial combination of any one of claims 6-9, a bacterial preparation of any one of claims 10-13 or a combination comprising same according to claims 14-16, a lysate of any one of claims 17-18, an extract of claim 19 or a whole cell broth of claim 20, further comprising at least one agriculturally acceptable diluent or carrier.
22. The agricultural composition of claim 21, wherein said composition further comprises an extract of at least one seaweed.
23. The agricultural composition of claim 22, wherein the seaweed is of a brown alga of a family selected from the group consisting of Fucaceae, Laminariaceae, Durvillaeaceae and Lessoniaceae.
24. The agricultural composition of claim 23, wherein the seaweed is selected from the group consisting of Ascophyllum nodosum, Laminaria hyperborean', Laminaria japonica', Laminaria digitata, Macrocystis pyrifera, Laminaria maxima, Durvillaea species and Lessonia species.
25. The agricultural composition of claim 24, wherein the seaweed is Acophyllus nodosum.
26. The agricultural composition of any one of claims 21-25, wherein said composition is a pesticidal composition.
27. The agricultural composition of any one of claims 21-26, wherein said composition further comprises at least one additional active agent selected from the group consisting of a fertilizer, an acaricide, a fungicide, an additional insecticide, an additional nematicide, a pesticide, a plant growth regulator, a rodenticide, a nutrient and any combination thereof.
28. The agricultural composition of claim 27, wherein the additional nematicide is selected from the group consisting of fluensulfone, abamectin, thiodicarb, benfuracarb, fluazinam, fluopiram, garlic extract, carbosulfan, caduzafos, terbufos, Pochonia, Pasteuria, Paecilomyces and Azadiractine.
29. The agricultural composition of claim 27, wherein the additional fungicide is selected from the group consisting of: copper fungicides selected from the group consisting of acypetacs-copper, Bordeaux mixture, Burgundy mixture, Cheshunt mixture, copper acetate, basic copper carbonate, copper hydroxide, copper naphthenate, copper oleate, copper oxychloride, copper silicate, copper sulfate, basic copper sulfate, copper zinc chromate, cufraneb, cuprobam, cuprous oxide, mancopper, oxine-copper, copper salt of bismerthiazol (saisentong), and thiodiazole-copper; morpholin fungicide selected from the group consisting ofdimethomorph, fenpropidin, and fenpropimorph; fosetyl-Al; DMI fungicide selected from the group consisting of tetraconazole tebuconazole, cyproconazole, difenoconazole, and flutriafol; propineb; Q0I (Strobilurin) fungicide selected from the group consisting of picoxystrobin, pyraclostrobin, and azoxystrobin; Benzimidazol fungicide selected from the group consisting of captan, carbendazim, tiophanat methyl, and thiabendazol; succinate dehydrogenase inhibitor (SDHI) fungicide; fluazinam; thiram; mancozeb; chlorotalony; potassium phosphite; mefenoxam; benlaxyl; matalaxyl; and folpet.
30. The agricultural composition of claim 27, wherein the additional insecticide is selected from the group consisting of fipronil, chlorantraniliprole, polyphenol, metaflumizone, bifenthrin, imidacloprid, thiametoxam, clothinidin, thiodicarb, thiacloprid, terbuphos, caduzaphos, ethiprole, dinotefuran, and benfuracarb.
31. The agricultural composition of any one of claims 21-30, wherein said agricultural composition is formulated in a form selected from the group consisting of a liquid, a solid, a semi-solid, a gel, a pressurized and a pressurizable form.
32. The agricultural composition of claim 31, wherein the formulation is in a form selected from the group consisting of wettable powders (WP), emulsion concentrates (EC), microemulsion concentrates (MEC), water-soluble powders (SP), water-soluble concentrates (SL), suspoemulsion (SE), oil dispersions (OD), concentrated emulsions (BW), sprayable solutions or emulsions, capsule suspensions (CS), suspension concentrates (SC), suspension concentrates, dusts (DP), oil-miscible solutions (OL), seed-dressing products, granules (GR), water-dispersible granules (WDG), ULV formulations, microcapsules, and waxes.
33. The agricultural composition of any one of claims 21-32, wherein said composition further comprises at least one of stabilizer, a tackifier, a preservative, a carrier, a surfactant, and a combination thereof.
34. The agricultural composition of claim 33, wherein the carrier is a plant seed.
35. The agricultural composition of claim 34, said composition is in a form of seed coating.
36. A plant seed coated with the agricultural composition of claim 35.
37. A kit comprising (i) an agricultural composition of any one of claims 2135; optionally (ii) a delivery system for applying the agricultural composition to a plant or a part thereof or to the plant growth habitat and (iii) instructions for using the agricultural composition.
38. A method for inhibiting or preventing the growth and / or the development of at least one nematode and / or plant pathogenic fungus and / or insect pest present in and / or on a plant or a part thereof or a plant habitat, comprising contacting the plant or part thereof or plant habitat with at least one isolated bacterial strain or functional homolog thereof of any one of claims 1-5, a microbial combination of any one of claims 6-9, a bacterial preparation of any one of claims 10-13, a lysate of any one of claims 17-18, an extract of claim 19, a whole cell broth of claim 20, or an agricultural composition of any one of claims 21-35.
39. A method for protecting a plant or a part thereof from a disease or deleterious symptoms associates with the growth and / or the development of at least one nematode and / or plant pathogenic fungus and / or insect pest in and / or on the plant or a part thereof or the plant habitat, comprising contacting said plant or part thereof or plant habitat with at least one isolated bacterial strain or functional homolog thereof of any one of claims 1-5, a microbial combination of any one of claims 6-9, a bacterial preparation of any one of claims 10-13, a lysate of any one of claims 17-18, an extract of claim 19 or a whole cell broth of claim 20, or an agricultural composition of any one of claims 21-35.
40. The method of any one of claims 38-39, wherein said method further comprises contacting said plant or part thereof or plant habitat with an extract of at least one seaweed.
41. The method of claim 40, wherein the seaweed is of a brown alga of a family selected from the group consisting of Fucaceae, Laminariaceae,Durvillaeaceae and Lessoniaceae.
42. The method of claim 40, wherein the seaweed is selected from the group consisting of Acophyllus nodosum, Laminaria hyperborean', Laminaria japonica', Laminaria digitata, Macrocystis pyrifera, Laminaria maxima, Durvillaea species and Lessonia species.
43. The method of any one of claims 38-42, wherein each of the bacterial strains, microbial preparation, lysate, extract or a whole broth obtained therefrom or a composition comprising same and the extract of at least one seaweed is applied separately.
44. The method of claim 43, wherein each of the bacterial strains, microbial preparation, lysate, extract or a whole broth obtained therefrom or a composition comprising same and the extract of at least one seaweed is applied contemporaneously or sequentially.
45. The method of any one of claims 38-42, wherein a combination of the bacterial strains, microbial preparation, lysate, extract or a whole broth obtained therefrom and the extract of at least one seaweed is applied within a single composition.
46. The method of any one of claims 38-45, wherein said method further comprises contacting the plant, part thereof or plant habitat with at least one additional nematicide and / or fungicide and / or pesticide.
47. The method of claim 46, wherein the at least one additional nematicide and / or fungicide and / or pesticide is applied together with the at least one bacterial strain, microbial preparation, lysate, extract or a whole broth obtained therefrom within the same pesticidal composition.
48. The method of claim 47, wherein each of the at least one bacterial strain, microbial preparation, lysate, extract or a whole broth obtained therefrom or a composition comprising same and the at least one additional nematicide and / or pesticide is applied separately.
49. The method of claim 48, wherein the at least one additional nematicide and / or fungicide and / or pesticide is applied contemporaneously orsubsequentially to the application of the at least one bacterial strain, microbial preparation, lysate, extract or a whole broth obtained therefrom or a composition comprising same.
50. The method of any one of claims 38-49, wherein the plant part is selected from the group consisting of a seed, a root, a shoot, a leaf, a branch, a flower, a fruit and any combination thereof.
51. The method of any one of claims 38-50, wherein the plant or part thereof is contacted with the bacterial strain, bacterial preparation, lysate, extract, whole cell broth or a composition comprising same by a method selected from the group consisting of infiltration, immersion, dipping, incubation, spraying, coating, dusting and any combination thereof.
52. The method of any one of claims 38-51, wherein the plant or part thereof is contacted with the bacterial preparation, lysate, extract, whole cell broth or agricultural composition comprising same indirectly, via administration of said bacterial preparation, lysate, extract, whole cell broth or agricultural composition comprising same to the plant habitat and / or growth medium.
53. The method according to claim 52, wherein the plant part is a seed and the bacterial preparation, lysate, extract, whole cell broth or agricultural composition comprising same is applied via seed coating.
54. The method of any one of claims 38-53, wherein the nematode is of a genus selected from the group consisting of Heterodera Meloidogyne, Pratylenchus, Helicotylenchus Xiphinema and Ditylenchus.
55. The method of any one of claims 38-53, wherein the insect pest is of an order selected from the group consisting of Lepidoptera, Coleopterci, Hemiptera.
56. The method of any one of claims 38-53, wherein the fungus is of a genus selected from the group consisting of Fusarium, Rhizoctonia, Sclerothinia, Macrophomina, Colletotrichum, Phomopsis, Aspergillus, Penicillium, and Cercopora.