Establishing method for rat central type superior mesenteric vein thrombosis model

Abstract

The invention relates to an establishing method for a rat central type superior mesenteric vein thrombosis model. The model is established by establishing an acute central type superior mesenteric vein thrombosis model, ligaturing the trunk root of a superior mesenteric vein at a time through a 7-0 no-damage nylon suture at the position 0.2 cm below the joint of the free superior mesenteric vein and a splenic vein, and conducting abdomen closing. A sub-acute central type superior mesenteric vein thrombosis model is established by placing a metal stick with the diameter of 1 mm and a superior mesenteric vein in parallel at the position 0.2 cm below the joint of the trunk of the free superior mesenteric vein and the splenic vein, ligaturing the superior mesenteric vein and the metal stick together through a 7-0 no-damage nylon suture, retreating the metal stick after ligation so that opened calibers of all rats can be kept basically consistent, and conducting abdomen closing. Food and water are freely fed in the next day. Abdomen opening is conducted again 48 hours later, the superior mesenteric vein is completely ligatured, and abdomen closing is conducted. Food and water are freely fed in the next day after the second operation. The rat central type superior mesenteric vein thrombosis model is suitable for superior mesenteric vein thrombosis intestinal pathology and pathophysiology research.

Description

technical field

[0001] The invention relates to a method for making an animal thrombosis model, in particular to a method for establishing a rat central superior mesenteric vein thrombosis model. Background technique

[0002] There are many ways to make animal thrombus models, but they basically follow the classic principle of thrombus formation proposed by Virchow, that is, changes in the vessel wall, changes in blood properties, and changes in the state of blood flow. At present, the medical field has focused on blocking venous return, causing blood stasis to induce thrombus formation, supplemented by injecting thrombus-inducing agents or (and) mechanically damaging vascular endothelial cells, and established some relatively reliable and easy animal models. The specific methods are: 1. vein ligation (blocking) method; 2. mechanical injury to vascular intima; 3. electric current injury to vascular intima; 4. venous foreign body method; Thrombosis induced by thrombosis, etc...

Examples

Embodiment Construction

[0027] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but these embodiments do not constitute any limitation to the present invention.

[0028] The method for setting up the rat central type superior mesenteric vein thrombosis model provided by the present embodiment is carried out according to the following steps:

[0029] 1. Instruments and equipment:

[0030] (l) OPTON binocular surgical microscope (OPTON53, Germany);

[0031] (2) Microsurgical instruments;

[0032] (3) General surgical instruments;

[0033] (4) 7-0 atraumatic nylon suture.

[0034] 2. Establishment of central superior mesenteric vein thrombosis model:

[0035] (1) Selection of experimental animals:

[0036] Select healthy adult male SD rats (provided by Beijing Huafukang Biotechnology Co., Ltd.), weighing 250g-300g, and raise them in a ventilated, clean environment at 20°C-25°C for one week, free to eat and drink;

[0037] (2) Take...

technical field

[0001] The invention relates to a method for making an animal thrombosis model, in particular to a method for establishing a rat central superior mesenteric vein thrombosis model. Background technique

[0002] There are many ways to make animal thrombus models, but they basically follow the classic principle of thrombus formation proposed by Virchow, that is, changes in the vessel wall, changes in blood properties, and changes in the state of blood flow. At present, the medical field has focused on blocking venous return, causing blood stasis to induce thrombus formation, supplemented by injecting thrombus-inducing agents or (and) mechanically damaging vascular endothelial cells, and established some relatively reliable and easy animal models. The specific methods are: 1. vein ligation (blocking) method; 2. mechanical injury to vascular intima; 3. electric current injury to vascular intima; 4. venous foreign body method; Thrombosis induced by thrombosis, etc...

Examples

Embodiment Construction

[0027] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but these embodiments do not constitute any limitation to the present invention.

[0028] The method for setting up the rat central type superior mesenteric vein thrombosis model provided by the present embodiment is carried out according to the following steps:

[0029] 1. Instruments and equipment:

[0030] (l) OPTON binocular surgical microscope (OPTON53, Germany);

[0031] (2) Microsurgical instruments;

[0032] (3) General surgical instruments;

[0033] (4) 7-0 atraumatic nylon suture.

[0034] 2. Establishment of central superior mesenteric vein thrombosis model:

[0035] (1) Selection of experimental animals:

[0036] Select healthy adult male SD rats (provided by Beijing Huafukang Biotechnology Co., Ltd.), weighing 250g-300g, and raise them in a ventilated, clean environment at 20°C-25°C for one week, free to eat and drink;

[0037] (2) Take...

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